Polynucleotides and polypeptides in plants

ABSTRACT

The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.

RELATIONSHIP TO COPENDING APPLICATIONS

This application is a divisional application of U.S. patent applicationSer. No. 12/338,024, filed on Dec. 18, 2008 (pending), which is adivisional application of U.S. patent application Ser. No. 10/374,780,filed on Feb. 25, 2003 (now U.S. Pat. No. 7,511,190). U.S. patentapplication Ser. No. 10/374,780 is a continuation-in-part of U.S. patentapplication Ser. No. 09/934,455, filed on Aug. 22, 2001 (now abandoned).U.S. patent application Ser. No. 09/934,455 is also acontinuation-in-part of U.S. patent application Ser. No. 09/837,944,filed on Apr. 18, 2001 (now abandoned). U.S. patent application Ser. No.10/374,780 is also a continuation-in-part of U.S. patent applicationSer. No. 10/225,068, filed on Aug. 9, 2002 (now U.S. Pat. No.7,193,129), which claims the benefit of U.S. provisional patentapplication Ser. No. 60/310,847, filed on Aug. 9, 2001. U.S. patentapplication Ser. No. 10/225,068 also claims the benefit of U.S.provisional patent application Ser. No. 60/336,049, filed on Nov. 19,2001, and the benefit of U.S. provisional patent application Ser. No.60/338,692, filed on Dec. 11, 2001. U.S. patent application Ser. No.10/225,068 is also a continuation-in-part of U.S. patent applicationSer. No. 09/837,944, filed on Apr. 18, 2001 (now abandoned). U.S. patentapplication Ser. No. 10/225,068 is also a continuation-in-part of U.S.patent application Ser. No. 10/171,468, filed on Jun. 14, 2002 (nowabandoned). U.S. patent application Ser. No. 10/374,780 is also acontinuation-in-part of U.S. patent application Ser. No. 10/225,066,filed on Aug. 9, 2002 (now U.S. Pat. No. 7,238,860). U.S. patentapplication Ser. No. 10/225,066 claims the benefit of U.S. provisionalpatent application Ser. No. 60/310,847, filed on Aug. 9, 2001. U.S.patent application Ser. No. 10/225,066 also claims the benefit of U.S.provisional patent application Ser. No. 60/336,049, filed on Nov. 19,2001, and the benefit of U.S. provisional patent application Ser. No.60/338,692, filed on Dec. 11, 2001. U.S. patent application Ser. No.10/225,066 is also a continuation-in-part of U.S. patent applicationSer. No. 09/837,944, filed on Apr. 18, 2001 (now abandoned). U.S. patentapplication Ser. No. 10/225,066 is also a continuation-in-part of U.S.patent application Ser. No. 10/171,468, filed on Jun. 14, 2002 (nowabandoned). U.S. patent application Ser. No. 10/374,780 is also acontinuation-in-part of U.S. patent application Ser. No. 10/225,067,filed on Aug. 9, 2002 (now U.S. Pat. No. 7,135,616). U.S. patentapplication Ser. No. 10/225,067 claims the benefit of U.S. provisionalpatent application Ser. No. 60/310,847, filed on Aug. 9, 2001, and thebenefit of U.S. provisional patent application Ser. No. 60/336,049,filed on Nov. 19, 2001, and the benefit of U.S. provisional patentapplication Ser. No. 60/338,692, filed on Dec. 11, 2001. U.S. patentapplication Ser. No. 10/225,067 is also a continuation-in-part of U.S.patent application Ser. No. 09/837,944, filed on Apr. 18, 2001 (nowabandoned). U.S. patent application Ser. No. 10/225,067 is also acontinuation-in-part of U.S. patent application Ser. No. 10/171,468,filed on Jun. 14, 2002 (now abandoned). All of the above-referencedpatent applications are incorporated herein by reference. U.S. patentapplication Ser. No. 10/374,780 is a continuation-in-part of U.S. patentapplication Ser. No. 09/713,994, filed on Nov. 16, 2000 (now abandoned),which claims the benefit of U.S. provisional patent application Ser. No.60/166,228, filed on Nov. 17, 1999. U.S. patent application Ser. No.09/713,994 also claims the benefit of provisional patent applicationSer. No. 60/197,899, filed on Apr. 17, 2000, and the benefit ofprovisional patent application Ser. No. 60/227,439, filed on Aug. 22,2000. U.S. patent application Ser. No. 10/374,780 is also acontinuation-in-part of U.S. patent application Ser. No. 09/934,455,filed on Aug. 22, 2001 (now abandoned), which claims the benefit of U.S.provisional patent application Ser. No. 60/227,439, filed on Aug. 22,2000. U.S. patent application Ser. No. 09/934,455 is also acontinuation-in-part of U.S. patent application Ser. No. 09/713,994,filed on Nov. 16, 2000 (now abandoned).

RESEARCH COLLABORATION

The claimed invention, in the field of functional genomics and thecharacterization of plant genes for the improvement of plants, was madeby or on behalf of Mendel Biotechnology, Inc. and Monsanto Company as aresult of activities undertaken within the scope of a joint researchagreement, said agreement having been in effect on or before the datethe claimed invention was made.

TECHNICAL FIELD

This invention relates to the field of plant biology. More particularly,the present invention pertains to compositions and methods for modifyinga plant phenotypically.

BACKGROUND OF THE INVENTION

A plant's traits, such as its biochemical, developmental, or phenotypiccharacteristics, may be controlled through a number of cellularprocesses. One important way to manipulate that control is throughtranscription factors—proteins that influence the expression of aparticular gene or sets of genes. Transformed and transgenic plants thatcomprise cells having altered levels of at least one selectedtranscription factor, for example, possess advantageous or desirabletraits. Strategies for manipulating traits by altering a plant cell'stranscription factor content can therefore result in plants and cropswith new and/or improved commercially valuable properties.

Transcription factors can modulate gene expression, either increasing ordecreasing (inducing or repressing) the rate of transcription. Thismodulation results in differential levels of gene expression at variousdevelopmental stages, in different tissues and cell types, and inresponse to different exogenous (e.g., environmental) and endogenousstimuli throughout the life cycle of the organism.

Because transcription factors are key controlling elements of biologicalpathways, altering the expression levels of one or more transcriptionfactors can change entire biological pathways in an organism. Forexample, manipulation of the levels of selected transcription factorsmay result in increased expression of economically useful proteins orbiomolecules in plants or improvement in other agriculturally relevantcharacteristics. Conversely, blocked or reduced expression of atranscription factor may reduce biosynthesis of unwanted compounds orremove an undesirable trait. Therefore, manipulating transcriptionfactor levels in a plant offers tremendous potential in agriculturalbiotechnology for modifying a plant's traits. A number of theagriculturally relevant characteristics of plants, and desirable traitsthat may be imbued by gene expression are listed below.

Useful Plant Traits

Category: Abiotic Stress; Desired Trait Chilling Tolerance

The term “chilling sensitivity” has been used to describe many types ofphysiological damage produced at low, but above freezing, temperatures.Most crops of tropical origins such as soybean, rice, maize and cottonare easily damaged by chilling. Typical chilling damage includeswilting, necrosis, chlorosis or leakage of ions from cell membranes. Theunderlying mechanisms of chilling sensitivity are not completelyunderstood yet, but probably involve the level of membrane saturationand other physiological deficiencies. For example, photoinhibition ofphotosynthesis (disruption of photosynthesis due to high lightintensities) often occurs under clear atmospheric conditions subsequentto cold late summer/autumn nights. By some estimates, chilling accountsfor monetary losses in the United States (US) second only to drought andflooding. For example, chilling may lead to yield losses and lowerproduct quality through the delayed ripening of maize. Anotherconsequence of poor growth is the rather poor ground cover of maizefields in spring, often resulting in soil erosion, increased occurrenceof weeds, and reduced uptake of nutrients. A retarded uptake of mineralnitrogen could also lead to increased losses of nitrate into the groundwater.

Category: Abiotic Stress; Desired Trait: Freezing Tolerance.

Freezing is a major environmental stress that limits where crops can begrown and reduces yields considerably, depending on the weather in aparticular growing season. In addition to exceptionally stressful yearsthat cause measurable losses of billions of dollars, less extreme stressalmost certainly causes smaller yield reductions over larger areas toproduce yield reductions of similar dollar value every year. Forinstance, in the US, the 1995 early fall frosts are estimated to havecaused losses of over one billion dollars to corn and soybeans. Thespring of 1998 saw an estimated $200 M of damages to Georgia alone, inthe peach, blueberry and strawberry industries. The occasional freezesin Florida have shifted the citrus belt further south due to $100 M ormore losses. California sustained $650 M of damage in 1998 to the citruscrop due to a winter freeze. In addition, certain crops such asEucalyptus, which has the very favorable properties of rapid growth andgood wood quality for pulping, are not able to grow in the southeasternstates due to occasional freezes.

Inherent winter hardiness of the crop determines in which agriculturalareas it can survive the winter. For example, for wheat, the northerncentral portion of the US has winters that are too cold for good winterwheat crops. Approximately 20% of the US wheat crop is spring wheat,with a market value of $2 billion. Areas growing spring wheat couldbenefit by growing winter wheat that had increased winter hardiness.Assuming a 25% yield increase when growing winter wheat, this wouldcreate $500 M of increased value. Additionally, the existing winterwheat is severely stressed by freezing conditions and should haveimproved yields with increased tolerance to these stresses. An estimateof the yield benefit of these traits is 10% of the $4.4 billion winterwheat crop in the US or $444 M of yield increase, as well as bettersurvival in extreme freezing conditions that occur periodically.

Thus plants more resistant to freezing, both midwinter freezing andsudden freezes, would protect a farmers' investment, improve yield andquality, and allow some geographies to grow more profitable andproductive crops. Additionally, winter crops such as canola, wheat andbarley have 25% to 50% yield increases relative to spring plantedvarieties of the same crops. This yield increase is due to the “headstart” the fall planted crop has over the spring planted crop and itsreaching maturity earlier while the temperatures, soil moisture and lackof pathogens provide more favorable conditions.

Category: Abiotic Stress; Desired Trait: Salt Tolerance.

One in five hectares of irrigated land is damaged by salt, an importanthistorical factor in the decline of ancient agrarian societies. Thiscondition is only expected to worsen, further reducing the availabilityof arable land and crop production, since none of the top five foodcrops—wheat, corn, rice, potatoes, and soybean—can tolerate excessivesalt.

Detrimental effects of salt on plants are a consequence of both waterdeficit resulting in osmotic stress (similar to drought stress) and theeffects of excess sodium ions on critical biochemical processes. As withfreezing and drought, high saline causes water deficit; the presence ofhigh salt makes it difficult for plant roots to extract water from theirenvironment (Buchanan et al. (2000) in Biochemistry and MolecularBiology of Plants, American Society of Plant Physiologists, Rockville,Md.). Soil salinity is thus one of the more important variables thatdetermines where a plant may thrive. In many parts of the world, sizableland areas are uncultivable due to naturally high soil salinity. Tocompound the problem, salination of soils that are used for agriculturalproduction is a significant and increasing problem in regions that relyheavily on agriculture. The latter is compounded by over-utilization,over-fertilization and water shortage, typically caused by climaticchange and the demands of increasing population. Salt tolerance is ofparticular importance early in a plant's lifecycle, since evaporationfrom the soil surface causes upward water movement, and salt accumulatesin the upper soil layer where the seeds are placed. Thus, germinationnormally takes place at a salt concentration much higher than the meansalt level in the whole soil profile.

Category: Abiotic Stress; Desired Trait: Drought Tolerance.

While much of the weather that we experience is brief and short-lived,drought is a more gradual phenomenon, slowly taking hold of an area andtightening its grip with time. In severe cases, drought can last formany years, and can have devastating effects on agriculture and watersupplies. With burgeoning population and chronic shortage of availablefresh water, drought is not only the number one weather related problemin agriculture, it also ranks as one of the major natural disasters ofall time, causing not only economic damage, but also loss of humanlives. For example, losses from the US drought of 1988 exceeded $40billion, exceeding the losses caused by Hurricane Andrew in 1992, theMississippi River floods of 1993, and the San Francisco earthquake in1989. In some areas of the world, the effects of drought can be far moresevere. In the Horn of Africa the 1984-1985 drought led to a famine thatkilled 750,000 people.

Problems for plants caused by low water availability include mechanicalstresses caused by the withdrawal of cellular water. Drought also causesplants to become more susceptible to various diseases (Simpson (1981).“The Value of Physiological Knowledge of Water Stress in Plants”, InWater Stress on Plants, (Simpson, G. M., ed.), Praeger, N.Y., pp.235-265).

In addition to the many land regions of the world that are too arid formost if not all crop plants, overuse and over-utilization of availablewater is resulting in an increasing loss of agriculturally-usable land,a process which, in the extreme, results in desertification. The problemis further compounded by increasing salt accumulation in soils, asdescribed above, which adds to the loss of available water in soils.

Category: Abiotic Stress; Desired Trait: Heat Tolerance.

Germination of many crops is very sensitive to temperature. Atranscription factor that would enhance germination in hot conditionswould be useful for crops that are planted late in the season or in hotclimates.

Seedlings and mature plants that are exposed to excess heat mayexperience heat shock, which may arise in various organs, includingleaves and particularly fruit, when transpiration is insufficient toovercome heat stress. Heat also damages cellular structures, includingorganelles and cytoskeleton, and impairs membrane function (Buchanan,supra).

Heat shock may result a decrease in overall protein synthesis,accompanied by expression of heat shock proteins. Heat shock proteinsfunction as chaperones and are involved in refolding proteins denaturedby heat.

Category: Abiotic Stress; Desired Trait: Tolerance to Low Nitrogen andPhosphorus.

The ability of all plants to remove nutrients from their environment isessential to survival. Thus, identification of genes that encodepolypeptides with transcription factor activity may allow for thegeneration of transgenic plants that are better able to make use ofavailable nutrients in nutrient-poor environments.

Among the most important macronutrients for plant growth that have thelargest impact on crop yield are nitrogenous and phosphorus-containingcompounds. Nitrogen- and phosphorus-containing fertilizers are usedintensively in agriculture practices today. An increase in grain cropyields from 0.5 to 1.0 metric tons per hectare to 7 metric tons perhectare accompanied the use of commercial fixed nitrogen fertilizer inproduction farming (Vance (2001) Plant Physiol. 127: 390-397). Givencurrent practices, in order to meet food production demands in years tocome, considerable increases in the amount of nitrogen- andphosphorus-containing fertilizers will be required (Vance, supra).

Nitrogen is the most abundant element in the Earth's atmosphere yet itis one of the most limiting elements to plant growth due to its lack ofavailability in the soil. Plants obtain N from the soil from severalsources including commercial fertilizers, manure and the mineralizationof organic matter. The intensive use of N fertilizers in presentagricultural practices is problematic, the energy intensive Haber-Boschprocess makes N fertilizer and it is estimated that the US uses annuallybetween 3-5% of the nation's natural gas for this process. In additionto the expense of N fertilizer production and the depletion ofnon-renewable resources, the use of N fertilizers has led to theeutrophication of freshwater ecosystems and the contamination ofdrinking water due to the runoff of excess fertilizer into ground watersupplies.

Phosphorus is second only to N in its importance as a macronutrient forplant growth and to its impact on crop yield. Phosphorus (P) isextremely immobile and not readily available to roots in the soil and istherefore often growth limiting to plants. Inorganic phosphate (Pi) is aconstituent of several important molecules required for energy transfer,metabolic regulation and protein activation (Marschner (1995) MineralNutrition of Higher Plants, 2nd ed., Academic Press, San Diego, Calif.).Plants have evolved several strategies to help cope with P and Ndeprivation that include metabolic as well as developmental adaptations.Most, if not all, of these strategies have components that are regulatedat the level of transcription and therefore are amenable to manipulationby transcription factors. Metabolic adaptations include increasing theavailability of P and N by increasing uptake from the soil though theinduction of high affinity and low affinity transporters, and/orincreasing its mobilization in the plant. Developmental adaptationsinclude increases in primary and secondary roots, increases in root hairnumber and length, and associations with mycorrhizal fungi (Bates andLynch (1996) Plant Cell Environ. 19: 529-538; Harrison (1999) Annu. Rev.Plant Physiol. Plant Mol. Biol. 50: 361-389).

Category: Biotic Stress; Desired Trait: Disease Resistance.

Disease management is a significant expense in crop productionworldwide. According to EPA reports for 1996 and 1997, US farmers spendapproximately $6 billion on fungicides annually. Despite thisexpenditure, according to a survey conducted by the food and agricultureorganization, plant diseases still reduce worldwide crop productivity by12% and in the United States alone, economic losses due to plantpathogens amounts to 9.1 billion dollars (FAO, 1993). Data from thesereports and others demonstrate that despite the availability of chemicalcontrol only a small proportion of the losses due to disease can beprevented. Not only are fungicides and anti-bacterial treatmentsexpensive to growers, but their widespread application poses bothenvironmental and health risks. The use of plant biotechnology toengineer disease resistant crops has the potential to make a significanteconomic impact on agriculture and forestry industries in two ways:reducing the monetary and environmental expense of fungicide applicationand reducing both pre-harvest and post-harvest crop losses that occurnow despite the use of costly disease management practices.

Fungal, bacterial, oomycete, viral, and nematode diseases of plants areubiquitous and important problems, and often severely impact yield andquality of crop and other plants. A very few examples of diseases ofplants include:

Powdery mildew, caused by the fungi Erysiphe, Sphaerotheca,Phyllactinia, Microsphaera, Podosphaera, or Uncinula, in, for example,wheat, bean, cucurbit, lettuce, pea, grape, tree fruit crops, as well asroses, phlox, lilacs, grasses, and Euonymus;

Fusarium-caused diseases such as Fusarium wilt in cucurbits, Fusariumhead blight in barley and wheat, wilt and crown and root rot intomatoes;

Sudden oak death, caused by the oomycete Phytophthora ramorum; thisdisease was first detected in 1995 in California tan oaks. The diseasehas since killed more than 100,000 tan oaks, coast live oaks, blackoaks, and Shreve's oaks in coastal regions of northern California, andmore recently in southwestern Oregon (Roach (2001) National GeographicNews, Dec. 6, 2001);

Black Sigatoka, a fungal disease caused by Mycosphaerella species thatattacks banana foliage, is spreading throughout the regions of the worldthat are responsible for producing most of the world's banana crop;

Eutypa dieback, caused by Eutypa lata, affects a number of crop plants,including vine grape. Eutypa dieback delays shoot emergence, and causeschlorosis, stunting, and tattering of leaves;

Pierce's disease, caused by the bacterium Xylella fastidiosa, precludesgrowth of grapes in the southeastern United States, and threatens theprofitable wine grape industry in northern California. The bacteriumclogs the vasculature of the grapevines, resulting in foliar scorchingfollowed by slow death of the vines. There is no known treatment forPierce's disease;

Bacterial Spot caused by the bacterium Xanthomonas campestris causesserious disease problems on tomatoes and peppers. It is a significantproblem in the Florida tomato industry because it spreads rapidly,especially in warm periods where there is wind-driven rain. Under theseconditions, there are no adequate control measures;

Diseases caused by viruses of the family Geminiviridae are a growingagricultural problem worldwide. Geminiviruses have caused severe croplosses in tomato, cassava, and cotton. For instance, in the 1991-1992growing season in Florida, geminiviruses caused $140 million in damagesto the tomato crop (Moffat (1991) Science 286: 1835). Geminiviruses havethe ability to recombine between strains to rapidly produce new virulentvarieties. Therefore, there is a pressing need for broad-spectrumgeminivirus control;

The soybean cyst nematode, Heterodera glycines, causes stunting andchlorosis of soybean plants, which results in yield losses or plantdeath from severe infestation. Annual losses in the United States havebeen estimated at $1.5 billion (University of Minnesota ExtensionService).

The aforementioned pathogens represent a very small fraction of diversespecies that seriously affect plant health and yield. For a morecomplete description of numerous plant diseases, see, for example,Vidhyasekaran (1997) Fungal Pathogenesis in Plants and Crops: MolecularBiology and Host Defense Mechanisms, Marcel Dekker, Monticello, N.Y.),or Agrios (1997) Plant Pathology, Academic Press, New York, N.Y.).Plants that are able to resist disease may produce significantly higheryields and improved food quality. It is thus of considerable importanceto find genes that reduce or prevent disease.

Category: Light Response; Desired Trait: Reduced Shade Avoidance.

Shade avoidance describes the process in which plants grown in closeproximity attempt to out-compete each other by increasing stem length atthe expense of leaf, fruit and storage organ development. This is causedby the plant's response to far-red radiation reflected from leaves ofneighboring plants, which is mediated by phytochrome photoreceptors.Close proximity to other plants, as is produced in high-density cropplantings, increases the relative proportion of far-red irradiation, andtherefore induces the shade avoidance response. Shade avoidanceadversely affects biomass and yield, particularly when leaves, fruits orother storage organs constitute the desired crop (see, for example,Smith (1982) Annu. Rev. Plant Physiol. 33: 481-518; Ballare et al.(1990) Science 247: 329-332; Smith (1995) Annu. Dev. Plant Physiol. Mol.Biol., 46: 289-315; and Schmitt et al. (1995), American Naturalist, 146:937-953). Alteration of the shade avoidance response in tobacco throughalteration of phytochrome levels has been shown to produce an increasein harvest index (leaf biomass/total biomass) at high planting density,which would result in higher yield (Robson et al. (1996) NatureBiotechnol. 14: 995-998).

Category: Flowering Time Desired Trait: Altered Flowering Time andFlowering Control.

Timing of flowering has a significant impact on production ofagricultural products. For example, varieties with different floweringresponses to environmental cues are necessary to adapt crops todifferent production regions or systems. Such a range of varieties havebeen developed for many crops, including wheat, corn, soybean, andstrawberry. Improved methods for alteration of flowering time willfacilitate the development of new, geographically adapted varieties.

Breeding programs for the development of new varieties can be limited bythe seed-to-seed cycle. Thus, breeding new varieties of plants withmulti-year cycles (such as biennials, e.g. carrot, or fruit trees, suchas citrus) can be very slow. With respect to breeding programs, therewould be a significant advantage in having commercially valuable plantsthat exhibit controllable and modified periods to flowering (“floweringtimes”). For example, accelerated flowering would shorten crop and treebreeding programs.

Improved flowering control allows more than one planting and harvest ofa crop to be made within a single season. Early flowering would alsoimprove the time to harvest plants in which the flower portion of theplant constitutes the product (e.g., broccoli, cauliflower, and otheredible flowers). In addition, chemical control of flowering throughinduction or inhibition of flowering in plants could provide asignificant advantage to growers by inducing more uniform fruitproduction (e.g., in strawberry)

A sizable number of plants for which the vegetative portion of the plantforms the valuable crop tend to “bolt” dramatically (e.g., spinach,onions, lettuce), after which biomass production declines and productquality diminishes (e.g., through flowering-triggered senescence ofvegetative parts). Delay or prevention of flowering may also reduce orpreclude dissemination of pollen from transgenic plants.

Category: Growth Rate; Desired Trait: Modified Growth Rate.

For almost all commercial crops, it is desirable to use plants thatestablish more quickly, since seedlings and young plants areparticularly susceptible to stress conditions such as salinity ordisease. Since many weeds may outgrow young crops or out-compete themfor nutrients, it would also be desirable to determine means forallowing young crop plants to out compete weed species. Increasingseedling growth rate (emergence) contributes to seedling vigor andallows for crops to be planted earlier in the season with less concernfor losses due to environmental factors. Early planting helps add daysto the critical grain-filling period and increases yield.

Providing means to speed up or slow down plant growth would also bedesirable to ornamental horticulture. If such means be provided, slowgrowing plants may exhibit prolonged pollen-producing or fruitingperiod, thus improving fertilization or extending harvesting season.

Category: Growth Rate; Desired Trait: Modified Senescence and CellDeath.

Premature senescence, triggered by various plant stresses, can limitproduction of both leaf biomass and seed yield. Transcription factorgenes that suppress premature senescence or cell death in response tostresses can provide means for increasing yield. Delay of normaldevelopmental senescence could also enhance yield, particularly forthose plants for which the vegetative part of the plant represents thecommercial product (e.g., spinach, lettuce).

Although leaf senescence is thought to be an evolutionary adaptation torecycle nutrients, the ability to control senescence in an agriculturalsetting has significant value. For example, a delay in leaf senescencein some maize hybrids is associated with a significant increase inyields and a delay of a few days in the senescence of soybean plants canhave a large impact on yield. In an experimental setting, tobacco plantsengineered to inhibit leaf senescence had a longer photosyntheticlifespan, and produced a 50% increase in dry weight and seed yield (Ganand Amasino (1995) Science 270: 1986-1988). Delayed flower senescencemay generate plants that retain their blossoms longer and this may be ofpotential interest to the ornamental horticulture industry, and delayedfoliar and fruit senescence could improve post-harvest shelf-life ofproduce.

Further, programmed cell death plays a role in other plant responses,including the resistance response to disease, and some symptoms ofdiseases, for example, as caused by necrotrophic pathogens such asBotrytis cinerea and Sclerotinia sclerotiorum (Dickman et al. Proc.Natl. Acad. Sci., 98: 6957-6962). Localized senescence and/or cell deathcan be used by plants to contain the spread of harmful microorganisms. Aspecific localized cell death response, the “hypersensitive response”,is a component of race-specific disease resistance mediated by plantresistance genes. The hypersensitive response is thought to help limitpathogen growth and to initiate a signal transduction pathway that leadsto the induction of systemic plant defenses. Accelerated senescence maybe a defense against obligate pathogens, such as powdery mildew, thatrely on healthy plant tissue for nutrients. With regard to powderymildew, Botrytis cinerea and Sclerotinia sclerotiorum and otherpathogens, transcription factors that ameliorate cell death and/ordamage may reduce the significant economic losses encountered, such as,for example, Botrytis cinerea in strawberry and grape.

Category: Growth Regulator; Desired Trait: Altered Sugar Sensing

Sugars are key regulatory molecules that affect diverse processes inhigher plants including germination, growth, flowering, senescence,sugar metabolism and photosynthesis. Sucrose, for example, is the majortransport form of photosynthate and its flux through cells has beenshown to affect gene expression and alter storage compound accumulationin seeds (source-sink relationships). Glucose-specific hexose-sensinghas also been described in plants and is implicated in cell division andrepression of “famine” genes (photosynthetic or glyoxylate cycles).

Category: Morphology; Desired Trait: Altered Morphology

Trichomes are branched or unbranched epidermal outgrowths or hairstructures on a plant. Trichomes produce a variety of secondarybiochemicals such as diterpenes and waxes, the former being importantas, for example, insect pheromones, and the latter as protectantsagainst desiccation and herbivorous pests. Since diterpenes also havecommercial value as flavors, aromas, pesticides and cosmetics, andpotential value as anti-tumor agents and inflammation-mediatingsubstances, they have been both products and the target of considerableresearch. In most cases where the metabolic pathways are impossible toengineer, increasing trichome density or size on leaves may be the onlyway to increase plant productivity. Thus, it would be advantageous todiscover trichome-affecting transcription factor genes for the purposeof increasing trichome density, size, or type to produce plants that arebetter protected from insects or that yield higher amounts of secondarymetabolites.

The ability to manipulate wax composition, amount, or distribution couldmodify plant tolerance to drought and low humidity or resistance toinsects, as well as plant appearance. In particular, a possibleapplication for a transcription factor gene that reduces wax productionin sunflower seed coats would be to reduce fouling during seed oilprocessing. Antisense or co-suppression of transcription factorsinvolved in wax biosynthesis in a tissue specific manner can be used tospecifically alter wax composition, amount, or distribution in thoseplants and crops from which wax is either a valuable attribute orproduct or an undesirable constituent of plants.

Other morphological characteristics that may be desirable in plantsinclude those of an ornamental nature. These include changes in seedcolor, overall color, leaf and flower shape, leaf color, leaf size, orglossiness of leaves. Plants that produce dark leaves may have benefitsfor human health; flavonoids, for example, have been used to inhibittumor growth, prevent of bone loss, and prevention lipid oxidation inanimals and humans. Plants in which leaf size is increased would likelyprovide greater biomass, which would be particularly valuable for cropsin which the vegetative portion of the plant constitutes the product.Plants with glossy leaves generally produce greater epidermal wax,which, if it could be augmented, resulted in a pleasing appearance formany ornamentals, help prevent desiccation, and resist herbivorousinsects and disease-causing agents. Changes in plant or plant partcoloration, brought about by modifying, for example, anthocyanin levels,would provide novel morphological features.

In many instances, the seeds of a plant constitute a valuable crop.These include, for example, the seeds of many legumes, nuts and grains.The discovery of means for producing larger seed would providesignificant value by bringing about an increase in crop yield.

Plants with altered inflorescence, including, for example, largerflowers or distinctive floral configurations, may have high value in theornamental horticulture industry.

Modifications to flower structure may have advantageous or deleteriouseffects on fertility, and could be used, for example, to decreasefertility by the absence, reduction or screening of reproductivecomponents. This could be a desirable trait, as it could be exploited toprevent or minimize the escape of the pollen of genetically modifiedorganisms into the environment.

Manipulation of inflorescence branching patterns may also be used toinfluence yield and offer the potential for more effective harvestingtechniques. For example, a “self pruning” mutation of tomato results ina determinate growth pattern and facilitates mechanical harvesting(Pnueli et al. (2001) Plant Cell 13(12): 2687-2702).

Alterations of apical dominance or plant architecture could create newplant varieties. Dwarf plants may be of potential interest to theornamental horticulture industry.

Category: Seed Biochemistry; Desired Trait: Altered Seed Oil

The composition of seeds, particularly with respect to seed oil quantityand/or composition, is very important for the nutritional value andproduction of various food and feed products. Desirable improvements tooils include enhanced heat stability, improved nutritional qualitythrough, for example, reducing the number of calories in seed,increasing the number of calories in animal feeds, or altering the ratioof saturated to unsaturated lipids comprising the oils.

Category: Seed Biochemistry; Desired Trait: Altered Seed Protein

As with seed oils, seed protein content and composition is veryimportant for the nutritional value and production of various food andfeed products. Altered protein content or concentration in seeds may beused to provide nutritional benefits, and may also prolong storagecapacity, increase seed pest or disease resistance, or modifygermination rates. Altered amino acid composition of seeds, throughaltered protein composition, is also a desired objective for nutritionalimprovement.

Category: Seed Biochemistry: Desired Trait: Altered Prenyl Lipids.

Prenyl lipids, including the tocopherols, play a role in anchoringproteins in membranes or membranous organelles. Tocopherols have bothanti-oxidant and vitamin E activity. Modified tocopherol composition ofplants may thus be useful in improving membrane integrity and function,which may mitigate abiotic stresses such as heat stress. Increasing theanti-oxidant and vitamin content of plants through increased tocopherolcontent can provide useful human health benefits.

Category: Leaf Biochemistry; Desired Trait: Altered Glucosinolate Levels

Increases or decreases in specific glucosinolates or total glucosinolatecontent can be desirable depending upon the particular application. Forexample: (i) glucosinolates are undesirable components of the oilseedsused in animal feed, since they produce toxic effects; low-glucosinolatevarieties of canola have been developed to combat this problem; (ii)some glucosinolates have anti-cancer activity; thus, increasing thelevels or composition of these compounds can be of use in production ofnutraceuticals; and (iii) glucosinolates form part of a plant's naturaldefense against insects; modification of glucosinolate composition orquantity could therefore afford increased protection from herbivores.Furthermore, tissue specific promoters can be used in edible crops toensure that these compounds accumulate specifically in particulartissues, such as the epidermis, which are not taken for humanconsumption.

Category: Leaf Biochemistry; Desired Trait: Flavonoid Production.

Expression of transcription factors that increase flavonoid productionin plants, including anthocyanins and condensed tannins, may be used toalter pigment production for horticultural purposes, and possibly toincrease stress resistance. Flavonoids have antimicrobial activity andcould be used to engineer pathogen resistance. Several flavonoidcompounds have human health promoting effects such as inhibition oftumor growth, prevention of bone loss and prevention of lipid oxidation.Increased levels of condensed tannins in forage legumes would provideagronomic benefits in ruminants by preventing pasture bloat bycollapsing protein foams within the rumen. For a review on the utilitiesof flavonoids and their derivatives, see Dixon et al. (1999) TrendsPlant Sci. 4: 394-400.

The present invention relates to methods and compositions for producingtransgenic plants with modified traits, particularly traits that addressthe agricultural and food needs described in the above backgroundinformation. These traits may provide significant value in that theyallow the plant to thrive in hostile environments, where, for example,temperature, water and nutrient availability or salinity may limit orprevent growth of non-transgenic plants. The traits may also comprisedesirable morphological alterations, larger or smaller size, disease andpest resistance, alterations in flowering time, light response, andothers.

We have identified polynucleotides encoding transcription factors,developed numerous transgenic plants using these polynucleotides, andhave analyzed the plants for a variety of important traits. In so doing,we have identified important polynucleotide and polypeptide sequencesfor producing commercially valuable plants and crops as well as themethods for making them and using them. Other aspects and embodiments ofthe invention are described below and can be derived from the teachingsof this disclosure as a whole.

SUMMARY OF THE INVENTION

Transgenic plants and methods for producing transgenic plants areprovided. The transgenic plants comprise a recombinant polynucleotidehaving a polynucleotide sequence, or a sequence that is complementary tothis polynucleotide sequence, that encodes a transcription factor.

The polynucleotide sequences that encode the transcription factors arelisted in the Sequence Listing and include any of any of SEQ ID NO:2N−1, wherein N=1-229, SEQ ID NO: 459-466; 468-487; 491-500; 504;506-511; 516-520; 523-524; 527; 529; 531-533; 538-539; 541-557; 560-568;570-586; 595-596; 598-606; 610-620; 627-634; 640-664; 670-707; 714-719;722-735; 740-741; 743-779; 808-823; 825-834; 838-850; 855-864; 868-889;892-902; 908-909; 914-921; 924-925; 927-932; 935-942; 944-952; 961-965;968-986; 989-993; 995-1010; 1012-1034; 1043-1063; 1074-1080; 1091-1104;1111-1121; 1123-1128; 1134-1138; 1142-1156; 1159-1175; 1187-1190;1192-1199; 1202-1220; 1249-1253; 1258-1262; 1264-1269; 1271-1287;1292-1301; 1303-1309; 1315-1323; 1328-1337; 1340-1341; 1344-1361;1365-1377; 1379-1390; 1393-1394; 1396-1398; 1419-1432; 1434-1452;1455-1456; 1460-1465; 1468-1491; 1499; 1502; 1505-1521; 1523-1527;1529-1532; 1536-1539; 1542-1562; 1567-1571; 1573-1582; 1587-1592;1595-1620; 1625-1644; 1647-1654; 1659-1669; 1671-1673; 1675-1680;1682-1686; 1688-1700; 1706-1709; 1714-1726; 1728-1734; 1738-1742;1744-1753; 1757-1760; 1763-1764; 1766-1768; 1770-1780; 1782-1784;1786-1789; 1791-1804; 1806-1812; 1814-1837; 1847-1856; 1858-1862;1864-1873; 1876-1882; 1885-1896; 1902-1910; 1913-1916; 1921-1928;1931-1936; 1940-1941; 1944-1946, or SEQ ID NO: 2N−1, wherein N=974-1101.

The transcription factors are comprised of polypeptide sequences listedin the Sequence Listing and include any of SEQ ID NO: 2N, whereinN=1-229, SEQ ID NO: 467; 488-490; 501-503; 505; 512-515; 521-522;525-526; 528; 530; 534-537; 540; 558-559; 569; 587-594; 597; 607-609;621-626; 635-639; 665-669; 708-713; 720-721; 736-739; 742; 780-807; 824;835-837; 851-854; 865-867; 890-891; 903-907; 910-913; 922-923; 926;933-934; 943; 953-960; 966-967; 987-988; 994; 1011; 1035-1042;1064-1073; 1081-1090; 1105-1110; 1122; 1129-1133; 1139-1141; 1157-1158;1176-1186; 1191; 1200-1201; 1221-1248; 1254-1257; 1263; 1270; 1288-1291;1302; 1310-1314; 1324-1327; 1338-1339; 1342-1343; 1362-1364; 1378;1391-1392; 1395; 1399-1418; 1433; 1453-1454; 1457-1459; 1466-1467;1492-1498; 1500-1501; 1503-1504; 1522; 1528; 1533-1535; 1540-1541;1563-1566; 1572; 1583-1586; 1593-1594; 1621-1624; 1645-1646; 1655-1658;1670; 1674; 1681; 1687; 1701-1705; 1710-1713; 1727; 1735-1737; 1743;1754-1756; 1761-1762; 1765; 1769; 1781; 1785; 1790; 1805; 1813;1838-1846; 1857; 1863; 1874-1875; 1883-1884; 1897-1901; 1911-1912;1917-1920; 1929-1930; 1937-1939; 1942-1943; or SEQ ID NO: 2N, whereinN=974-1101.

The transgenic plant that comprises the recombinant polynucleotide has apolynucleotide sequence, or a sequence that is complementary to thispolynucleotide sequence, selected from any of the following:

(a) a polynucleotide sequence that encodes one of the transcriptionfactor polypeptide sequences of Paragraph 2 of this Summary; or

(b) a polynucleotide sequence that comprises one of the polynucleotidesequences of paragraph 3 of this Summary.

The transgenic plant may also comprise a polynucleotide sequence that isa variant of the sequences in (a) and (b) that encode a polypeptide andregulate transcription, including:

(c) a sequence variant of the polynucleotide sequences of (a) or (b);

(d) an allelic variant of the polynucleotide sequences of (a) or (b);

(e) a splice variant of the polynucleotide sequences of (a) or (b);

(f) an orthologous sequence of the polynucleotide sequences of (a) or(b);

(g) a paralogous sequence of the polynucleotide sequences of (a) or (b);

(h) a polynucleotide sequence encoding a polypeptide comprising aconserved domain that exhibits at least 70% sequence homology with thepolypeptide of (a), and the polypeptide comprises a conserved domain ofa transcription factor that regulates transcription; or

(i) a polynucleotide sequence that hybridizes under stringent conditionsto a polynucleotide sequence of one or more polynucleotides of (a) or(b), and the polynucleotide sequence encodes a polypeptide thatregulates transcription.

A transcription factor sequence variant is one having at least 26% aminoacid sequence similarity, or at least 40% amino acid sequence identity.A preferred transcription factor sequence variant is one having at least50% amino acid sequence identity and a more preferred transcriptionfactor sequence variant is one having at least 65% amino acid sequenceidentity to the transcription factor polypeptide sequences of paragraph3 of this Summary, and that contains at least one functional orstructural characteristic of the similar transcription factorpolypeptide sequences. Sequences having lesser degrees of identity butcomparable biological activity are considered to be equivalents.

The transcription factor polypeptides of the present invention includeat least one conserved domain, and the portions of the polynucleotidesequences encoding the conserved domain generally exhibit at least 70%sequence identity with the aforementioned preferred polynucleotidesequences. In the case of zinc finger transcription factors, the percentidentity across the conserved domain may be as low as 50%.

Various types of plants may be used to generate the transgenic plants,including soybean, wheat, corn, potato, cotton, rice, oilseed rape,sunflower, alfalfa, clover, sugarcane, turf, banana, blackberry,blueberry, strawberry, raspberry, cantaloupe, carrot, cauliflower,coffee, cucumber, eggplant, grapes, honeydew, lettuce, mango, melon,onion, papaya, peas, peppers, pineapple, pumpkin, spinach, squash, sweetcorn, tobacco, tomato, watermelon, mint and other labiates, rosaceousfruits, and vegetable brassicas.

The transgenic plant may be monocotyledonous, plant, and thepolynucleotide sequences used to transform the transgenic plant may bederived from either a monocot or a dicot plant. Alternatively, thetransgenic plant may be a dicotyledonous plant, and the polynucleotidesequences used to transform the transgenic plant may be derived fromeither a monocot or a dicot plant.

These transgenic plants will generally possess traits that are alteredas compared to a control plant, such as a wild-type or non-transformedplant (i.e., the non-transformed plant does not comprise the recombinantpolynucleotide), thus producing an phenotype that is altered whencompared to the control, wild-type or non-transformed plant. Thesetransgenic plants may also express an altered level of one or more genesassociated with a plant trait as compared to the non-transformed plant.The encoded polypeptides in these transgenic plants will generally beexpressed and regulate transcription of at least one gene; this genewill generally confer at least one altered trait, phenotype orexpression level.

Any of the polynucleotide sequences listed in the Sequence Listing,their complements, and functional variants used to transform thetransgenic plants of the present invention may further compriseregulatory elements. The regulatory elements, may comprise, for example,constitutive, inducible, or tissue-specific promoters operably linked toa polynucleotide sequence.

Presently disclosed transcription factor sequences may be used toproduce transformed plants with a variety of improved traits. An exampleof such an altered trait is enhanced tolerance to abiotic stress, suchas salt tolerance, chilling conditions, and drought conditions. Salt anddrought tolerance, both forms of osmotic stress, may be mediatedin partby increased root growth or increased root hairs relative to anon-transformed, control or wild-type plant. Tolerance to abioticstresses such as salt, chilling and drought tolerance may confer anumber of survival, quality and yield improvements, including improvedseed germination and improved seedling vigor, plant survival, as well asimproved yield, quality, and range.

Another example of an altered trait that may be conferred bytransforming plants with the presently disclosed transcription factorsequences includes altered sugar sensing. Altered sugar sensing may alsobe used to confer improved seed germination and improved seedling vigor,as well as altered flowering, senescence, sugar metabolism andphotosynthesis characteristics.

The invention also pertains to method to produce these transgenicplants.

The present invention also relates to a method of using transgenicplants transformed with the presently disclosed transcription factorsequences, their complements or their variants to grow a progeny plantby crossing the transgenic plant with either itself or another plant,selecting seed that develops as a result of the crossing; and thengrowing the progeny plant from the seed. The progeny plant willgenerally express mRNA that encodes a transcription factor: that is, aDNA-binding protein that binds to a DNA regulatory sequence andregulates gene expression, such as that of a plant trait gene. The mRNAwill generally be expressed at a level greater than a non-transformedplant; and the progeny plant is characterized by a change in a planttrait compared to the non-transformed plant.

The present invention also pertains to an expression cassette. Theexpression cassette comprises at least two elements, including:

(1) a constitutive, inducible, or tissue-specific promoter; and

(2) a recombinant polynucleotide having a polynucleotide sequence, or acomplementary polynucleotide sequence thereof, selected from the groupconsisting of a polynucleotide sequence encoding a (a) polypeptidesequence selected from the transcription factor sequences in the thirdparagraph of this Summary; or (b) a polynucleotide sequence selectedfrom the transcription factor polynucleotides of second paragraph ofthis Summary, or (c) sequence variants such as allelic or splicevariants of the polynucleotide sequences of (a) or (b), where thesequence variant encodes a polypeptide that regulates transcription. Thepolynucleotide sequence may also comprise an orthologous or paralogoussequence of the polynucleotide sequences of (a) or (b), with thesesequences encoding a polypeptide that regulates transcription, apolynucleotide sequence that encoding a polypeptide having a conserveddomain that exhibits 72% or greater sequence homology with thepolypeptide of (a), where the polypeptide comprising the conserveddomain regulates transcription, or a polynucleotide sequence thathybridizes under stringent conditions to a polynucleotide sequence ofone or more polynucleotides of (a) or (b), where the latterpolynucleotide sequence regulates transcription. In all of these cases,the recombinant polynucleotide is operably linked to the promoter of theexpression cassette.

The invention also includes a host cell that comprises the expressioncassette. The host cell may be a plant cell, such as, for example, acell of a crop plant.

The invention also concerns a method for identifying a factor that ismodulated by or interacts with a polypeptide of the third paragraph ofthis Summary. This method is conducted by: expressing the polypeptide ina plant; and then identifying at least one factor that is modulated byor interacts with the polypeptide.

The invention also pertains to a method for identifying at least onedownstream polynucleotide sequence that is subject to a regulatoryeffect of any of the polypeptides of the third paragraph of thisSummary. This method includes expressing any of the polypeptides of thethird paragraph of this Summary in a plant cell; and then identifyingresultant RNA or protein. The latter identification may be carried outwith, for example, such methods that include Northern analysis, RT-PCR,microarray gene expression assays, reporter gene expression systemssubtractive hybridization, differential display, representationaldifferential analysis, or two-dimensional gel electrophoresis of one ormore protein products.

The invention also provides a transgenic plant comprising apolynucleotide encoding a polypeptide with a conserved domain, whereinthe conserved domain comprises consecutive amino acid residuesSer-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Glu/Asp-Glu-Glu/Asp-Glu/Asp-Ala-Ala/Val-Arg-Ala/Ser-Tyr-Asp-Val/Ile-Ala/Val-Val/Ala-Xaa-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn-Phe-Lys/Argof SEQ ID NO:170, wherein Xaa is any amino acid residue. The inventionstill further provides a transgenic plant comprising a polynucleotidewherein the polynucleotide sequence is selected from the groupconsisting of SEQ ID NO: 169, 369, 1159 through 1175, 1949, and 2071. Inanother embodiment, the invention also provides a transgenic plantcomprising a polynucleotide encoding a polypeptide, wherein thepolypeptide is selected from the group consisting of SEQ ID NO: 170,370, 1176 through 1186, 1950, and 2072.

The invention also provides an expression cassette comprising apolynucleotide encoding a polypeptide with a conserved domain, whereinthe conserved domain comprises consecutive amino acid residuesSer-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Glu/Asp-Glu-Glu/Asp-Glu/Asp-Ala-Ala/Val-Arg-Ala/Ser-Tyr-Asp-Val/Ile-Ala/Val-Val/Ala-Xaa-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn-Phe-Lys/Argof SEQ ID NO:170, wherein Xaa is any amino acid residue. The inventionstill further provides an expression cassette comprising apolynucleotide sequence is selected from the group consisting of SEQ IDNO: 169, 369, 1159 through 1175, 1949, and 2071. In another embodiment,the invention also provides an expression cassette comprising apolynucleotide encoding a polypeptide, wherein the polypeptide isselected from the group consisting of SEQ ID NO: 170, 370, 1176 through1186, 1950, and 2072.

The invention also provides a method for producing a modified planthaving a polynucleotide encoding a polypeptide with a conserved domain,wherein the conserved domain comprises consecutive amino acid residuesSer-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Glu/Asp-Glu-Glu/Asp-Glu/Asp-Ala-Ala/Val-Arg-Ala/Ser-Tyr-Asp-Val/Ile-Ala/Val-Val/Ala-Xaa-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn-Phe-Lys/Argof SEQ ID NO:170, wherein Xaa is any amino acid residue. The inventionstill further provides a method for producing a modified plant having apolynucleotide, wherein the polynucleotide sequence is selected from thegroup consisting of SEQ ID NO: 169, 369, 1159 through 1175, 1949, and2071. In another embodiment, the invention also provides a method forproducing a modified plant having a polynucleotide encoding apolypeptide, wherein the polypeptide is selected from the groupconsisting of SEQ ID NO: 170, 370, 1176 through 1186, 1950, and 2072.

BRIEF DESCRIPTION OF THE SEQUENCE LISTING AND DRAWINGS

The Sequence Listing provides exemplary polynucleotide and polypeptidesequences of the invention. The traits associated with the use of thesequences are included in the Examples.

A computer-readable format (CRF) of a Sequence Listing is provided inASCII text format. The Sequence Listing is named“MBI0047-2DIV_ST25.txt”, file creation date of Feb. 6, 2012, and is6,408,566 bytes in size (6,259 kilobytes in size as measured by MSWindows). The Sequence Listing is hereby incorporated by reference intheir its entirety.

FIG. 1 shows a conservative estimate of phylogenetic relationships amongthe orders of flowering plants (modified from Angiosperm Phylogeny Group(1998) Ann. Missouri Bot. Gard. 84: 1-49). Those plants with a singlecotyledon (monocots) are a monophyletic clade nested within at least twomajor lineages of dicots; the eudicots are further divided into rosidsand asterids. Arabidopsis is a rosid eudicot classified within the orderBrassicales; rice is a member of the monocot order Poales. FIG. 1 wasadapted from Daly et al. (2001) Plant Physiol. 127: 1328-1333.

FIG. 2 shows a phylogenic dendogram depicting phylogenetic relationshipsof higher plant taxa, including clades containing tomato andArabidopsis; adapted from Ku et al. (2000) Proc. Natl. Acad. Sci. 97:9121-9126; and Chase et al. (1993) Ann. Missouri Bot. Gard. 80: 528-580.

FIGS. 3A, and 3B show an alignment of G682 (SEQ ID NO: 148) andpolynucleotide sequences that are paralogous and orthologous to G682.The alignment was produced using MACVECTOR software (Accelrys, Inc., SanDiego, Calif.).

FIGS. 4A, 4B, 4C and 4D show an alignment of G867 (SEQ ID NO: 170) andpolynucleotide sequences that are paralogous and orthologous to G867.The alignment was produced using MACVECTOR software (Accelrys, Inc.).

FIGS. 5A, 5B, 5C, 5D, 5E and 5F show an alignment of G912 (SEQ ID NO:186) and polynucleotide sequences that are paralogous and orthologous toG912. The alignment was produced using MACVECTOR software (Accelrys,Inc.).

DETAILED DESCRIPTION OF EXEMPLARY EMBODIMENTS

In an important aspect, the present invention relates to polynucleotidesand polypeptides, for example, for modifying phenotypes of plants.Throughout this disclosure, various information sources are referred toand/or are specifically incorporated. The information sources includescientific journal articles, patent documents, textbooks, and World WideWeb browser-inactive page addresses, for example. While the reference tothese information sources clearly indicates that they can be used by oneof skill in the art, each and every one of the information sources citedherein are specifically incorporated in their entirety, whether or not aspecific mention of “incorporation by reference” is noted. The contentsand teachings of each and every one of the information sources can berelied on and used to make and use embodiments of the invention.

It must be noted that as used herein and in the appended claims, thesingular forms “a,” “an,” and “the” include plural reference unless thecontext clearly dictates otherwise. Thus, for example, a reference to “aplant” includes a plurality of such plants, and a reference to “astress” is a reference to one or more stresses and equivalents thereofknown to those skilled in the art, and so forth.

The polynucleotide sequences of the invention encode polypeptides thatare members of well-known transcription factor families, including planttranscription factor families, as disclosed in Tables 4-5. Generally,the transcription factors encoded by the present sequences are involvedin cellular metabolism, cell differentiation and proliferation and theregulation of growth. Accordingly, one skilled in the art wouldrecognize that by expressing the present sequences in a plant, one maychange the expression of autologous genes or induce the expression ofintroduced genes. By affecting the expression of similar autologoussequences in a plant that have the biological activity of the presentsequences, or by introducing the present sequences into a plant, one mayalter a plant's phenotype to one with improved traits. The sequences ofthe invention may also be used to transform a plant and introducedesirable traits not found in the wild-type cultivar or strain. Plantsmay then be selected for those that produce the most desirable degree ofover- or under-expression of target genes of interest and coincidenttrait improvement.

The sequences of the present invention may be from any species,particularly plant species, in a naturally occurring form or from anysource whether natural, synthetic, semi-synthetic or recombinant. Thesequences of the invention may also include fragments of the presentamino acid sequences. In this context, a “fragment” refers to a fragmentof a polypeptide sequence which is at least 5 to about 15 amino acids inlength, most preferably at least 14 amino acids, and which retain somebiological activity of a transcription factor. Where “amino acidsequence” is recited to refer to an amino acid sequence of a naturallyoccurring protein molecule, “amino acid sequence” and like terms are notmeant to limit the amino acid sequence to the complete native amino acidsequence associated with the recited protein molecule.

As one of ordinary skill in the art recognizes, transcription factorscan be identified by the presence of a region or domain of structuralsimilarity or identity to a specific consensus sequence or the presenceof a specific consensus DNA-binding site or DNA-binding site motif (see,for example, Riechmann et al. (2000) Science 290: 2105-2110). The planttranscription factors may belong to one of the following transcriptionfactor families: the AP2 (APETALA2) domain transcription factor family(Riechmann and Meyerowitz (1998) Biol. Chem. 379: 633-646); the MYBtranscription factor family (ENBib; Martin and Paz-Ares (1997) TrendsGenet. 13: 67-73); the MADS domain transcription factor family(Riechmann and Meyerowitz (1997) Biol. Chem. 378: 1079-1101); the WRKYprotein family (Ishiguro and Nakamura (1994) Mol. Gen. Genet. 244:563-571); the ankyrin-repeat protein family (Zhang et al. (1992) PlantCell 4: 1575-1588); the zinc finger protein (Z) family (Klug and Schwabe(1995) FASEB J. 9: 597-604); Takatsuji (1998) Cell. Mol. Life. Sci.54:582-596); the homeobox (HB) protein family (Buerglin (1994) inGuidebook to the Homeobox Genes, Duboule (ed.) Oxford University Press);the CAAT-element binding proteins (Forsburg and Guarente (1989) GenesDev. 3: 1166-1178); the squamosa promoter binding proteins (SPB) (Kleinet al. (1996) Mol. Gen. Genet. 1996 250: 7-16); the NAM protein family(Souer et al. (1996) Cell 85: 159-170); the IAA/AUX proteins (Abel etal. (1995) J. Mol. Biol. 251: 533-549); the HLH/MYC protein family(Littlewood et al. (1994) Prot. Profile 1: 639-709); the DNA-bindingprotein (DBP) family (Tucker et al. (1994) EMBO J. 13: 2994-3002); thebZIP family of transcription factors (Foster et al. (1994) FASEB J. 8:192-200); the Box P-binding protein (the BPF-1) family (da Costa e Silvaet al. (1993) Plant J. 4: 125-135); the high mobility group (HMG) family(Bustin and Reeves (1996) Prog. Nucl. Acids Res. Mol. Biol. 54: 35-100);the scarecrow (SCR) family (Di Laurenzio et al. (1996) Cell 86:423-433); the GF14 family (Wu et al. (1997) Plant Physiol. 114:1421-1431); the polycomb (PCOMB) family (Goodrich et al. (1997) Nature386: 44-51); the teosinte branched (TEO) family (Luo et al. (1996)Nature 383: 794-799); the ABI3 family (Giraudat et al. (1992) Plant Cell4: 1251-1261); the triple helix (TH) family (Dehesh et al. (1990)Science 250: 1397-1399); the EIL family (Chao et al. (1997) Cell 89:1133-44); the AT-HOOK family (Reeves and Nissen (1990) J. Biol. Chem.265: 8573-8582); the S1FA family (Thou et al. (1995) Nucleic Acids Res.23: 1165-1169); the bZIPT2 family (Lu and Ferl (1995) Plant Physiol.109: 723); the YABBY family (Bowman et al. (1999) Development 126:2387-96); the PAZ family (Bohmert et al. (1998) EMBO J. 17: 170-80); afamily of miscellaneous (MISC) transcription factors including the DPBFfamily (Kim et al. (1997) Plant J. 11: 1237-1251) and the SPF1 family(Ishiguro and Nakamura (1994) Mol. Gen. Genet. 244: 563-571); the GARPfamily (Hall et al. (1998) Plant Cell 10: 925-936), the TUBBY family(Boggin et al (1999) Science 286: 2119-2125), the heat shock family (Wu(1995) Annu. Rev. Cell Dev. Biol. 11: 441-469), the ENBP family(Christiansen et al. (1996) Plant Mol. Biol. 32: 809-821), the RING-zincfamily (Jensen et al. (1998) FEBS Letters 436: 283-287), the PDBP family(Janik et al. (1989) Virology 168: 320-329), the PCF family (Cubas etal. Plant J. (1999) 18: 215-22), the SRS (SHI-related) family (Fridborget al. (1999) Plant Cell 11: 1019-1032), the CPP (cysteine-richpolycomb-like) family (Cvitanich et al. (2000) Proc. Natl. Acad. Sci.97: 8163-8168), the ARF (auxin response factor) family (Ulmasov et al.(1999) Proc. Natl. Acad. Sci. 96: 5844-5849), the SWI/SNF family(Collingwood et al. (1999) J. Mol. Endocrinol. 23: 255-275), the ACBFfamily (Seguin et al. (1997) Plant Mol. Biol. 35: 281-291), PCGL (CG-1like) family (da Costa e Silva et al. (1994) Plant Mol. Biol. 25:921-924) the ARID family (Vazquez et al. (1999) Development 126:733-742), the Jumonji family (Balciunas et al. (2000), Trends Biochem.Sci. 25: 274-276), the bZIP-NIN family (Schauser et al. (1999) Nature402: 191-195), the E2F family (Kaelin et al. (1992) Cell 70: 351-364)and the GRF-like family (Knaap et al. (2000) Plant Physiol. 122:695-704). As indicated by any part of the list above and as known in theart, transcription factors have been sometimes categorized by class,family, and sub-family according to their structural content andconsensus DNA-binding site motif, for example. Many of the classes andmany of the families and sub-families are listed here. However, theinclusion of one sub-family and not another, or the inclusion of onefamily and not another, does not mean that the invention does notencompass polynucleotides or polypeptides of a certain family orsub-family. The list provided here is merely an example of the types oftranscription factors and the knowledge available concerning theconsensus sequences and consensus DNA-binding site motifs that helpdefine them as known to those of skill in the art (each of thereferences noted above are specifically incorporated herein byreference). A transcription factor may include, but is not limited to,any polypeptide that can activate or repress transcription of a singlegene or a number of genes. This polypeptide group includes, but is notlimited to, DNA-binding proteins, DNA-binding protein binding proteins,protein kinases, protein phosphatases, protein methyltransferases,GTP-binding proteins, and receptors, and the like.

In addition to methods for modifying a plant phenotype by employing oneor more polynucleotides and polypeptides of the invention describedherein, the polynucleotides and polypeptides of the invention have avariety of additional uses. These uses include their use in therecombinant production (i.e., expression) of proteins; as regulators ofplant gene expression, as diagnostic probes for the presence ofcomplementary or partially complementary nucleic acids (including fordetection of natural coding nucleic acids); as substrates for furtherreactions, e.g., mutation reactions, PCR reactions, or the like; assubstrates for cloning e.g., including digestion or ligation reactions;and for identifying exogenous or endogenous modulators of thetranscription factors. A “polynucleotide” is a nucleic acid moleculecomprising a plurality of polymerized nucleotides, e.g., at least about15 consecutive polymerized nucleotides, optionally at least about 30consecutive nucleotides, at least about 50 consecutive nucleotides. Apolynucleotide may be a nucleic acid, oligonucleotide, nucleotide, orany fragment thereof. In many instances, a polynucleotide comprises anucleotide sequence encoding a polypeptide (or protein) or a domain orfragment thereof. Additionally, the polynucleotide may comprise apromoter, an intron, an enhancer region, a polyadenylation site, atranslation initiation site, 5′ or 3′ untranslated regions, a reportergene, a selectable marker, or the like. The polynucleotide can be singlestranded or double stranded DNA or RNA. The polynucleotide optionallycomprises modified bases or a modified backbone. The polynucleotide canbe, e.g., genomic DNA or RNA, a transcript (such as an mRNA), a cDNA, aPCR product, a cloned DNA, a synthetic DNA or RNA, or the like. Thepolynucleotide can be combined with carbohydrate, lipids, protein, orother materials to perform a particular activity such as transformationor form a useful composition such as a peptide nucleic acid (PNA). Thepolynucleotide can comprise a sequence in either sense or antisenseorientations. “Oligonucleotide” is substantially equivalent to the termsamplimer, primer, oligomer, element, target, and probe and is preferablysingle stranded.

DEFINITIONS

A “recombinant polynucleotide” is a polynucleotide that is not in itsnative state, e.g., the polynucleotide comprises a nucleotide sequencenot found in nature, or the polynucleotide is in a context other thanthat in which it is naturally found, e.g., separated from nucleotidesequences with which it typically is in proximity in nature, or adjacent(or contiguous with) nucleotide sequences with which it typically is notin proximity. For example, the sequence at issue can be cloned into avector, or otherwise recombined with one or more additional nucleicacid.

An “isolated polynucleotide” is a polynucleotide whether naturallyoccurring or recombinant, that is present outside the cell in which itis typically found in nature, whether purified or not. Optionally, anisolated polynucleotide is subject to one or more enrichment orpurification procedures, e.g., cell lysis, extraction, centrifugation,precipitation, or the like.

A “polypeptide” is an amino acid sequence comprising a plurality ofconsecutive polymerized amino acid residues e.g., at least about 15consecutive polymerized amino acid residues, optionally at least about30 consecutive polymerized amino acid residues, at least about 50consecutive polymerized amino acid residues. In many instances, apolypeptide comprises a polymerized amino acid residue sequence that isa transcription factor or a domain or portion or fragment thereof. Atranscription factor can regulate gene expression and may increase ordecrease gene expression in a plant. Additionally, the polypeptide maycomprise 1) a localization domain, 2) an activation domain, 3) arepression domain, 4) an oligomerization domain, or 5) a DNA-bindingdomain, or the like. The polypeptide optionally comprises modified aminoacid residues, naturally occurring amino acid residues not encoded by acodon, non-naturally occurring amino acid residues.

A “recombinant polypeptide” is a polypeptide produced by translation ofa recombinant polynucleotide. A “synthetic polypeptide” is a polypeptidecreated by consecutive polymerization of isolated amino acid residuesusing methods well known in the art. An “isolated polypeptide,” whethera naturally occurring or a recombinant polypeptide, is more enriched in(or out of) a cell than the polypeptide in its natural state in awild-type cell, e.g., more than about 5% enriched, more than about 10%enriched, or more than about 20%, or more than about 50%, or more,enriched, i.e., alternatively denoted: 105%, 110%, 120%, 150% or more,enriched relative to wild type standardized at 100%. Such an enrichmentis not the result of a natural response of a wild-type plant.Alternatively, or additionally, the isolated polypeptide is separatedfrom other cellular components with which it is typically associated,e.g., by any of the various protein purification methods herein.

“Identity” or “similarity” refers to sequence similarity between twopolynucleotide sequences or between two polypeptide sequences, withidentity being a more strict comparison. The phrases “percent identity”and “% identity” refer to the percentage of sequence similarity found ina comparison of two or more polynucleotide sequences or two or morepolypeptide sequences. “Sequence similarity” refers to the percentsimilarity in base pair sequence (as determined by any suitable method)between two or more polynucleotide sequences. Two or more sequences canbe anywhere from 0-100% similar, or any integer value therebetween.Identity or similarity can be determined by comparing a position in eachsequence that may be aligned for purposes of comparison. When a positionin the compared sequence is occupied by the same nucleotide base oramino acid, then the molecules are identical at that position. A degreeof similarity or identity between polynucleotide sequences is a functionof the number of identical or matching nucleotides at positions sharedby the polynucleotide sequences. A degree of identity of polypeptidesequences is a function of the number of identical amino acids atpositions shared by the polypeptide sequences. A degree of homology orsimilarity of polypeptide sequences is a function of the number of aminoacids at positions shared by the polypeptide sequences.

“Alignment” refers to a number of DNA or amino acid sequences aligned bylengthwise comparison so that components in common (i.e., nucleotidebases or amino acid residues) may be visually and readily identified.The fraction or percentage of components in common is related to thehomology or identity between the sequences. Alignments such as those ofFIG. 3, 4, or 5 may be used to identify conserved domains andrelatedness within these domains. An alignment may suitably bedetermined by means of computer programs known in the art, such asMACVECTOR software (1999) (Accelrys, Inc., San Diego, Calif.).

The terms “highly stringent” or “highly stringent condition” refer toconditions that permit hybridization of DNA strands whose sequences arehighly complementary, wherein these same conditions excludehybridization of significantly mismatched DNAs. Polynucleotide sequencescapable of hybridizing under stringent conditions with thepolynucleotides of the present invention may be, for example, variantsof the disclosed polynucleotide sequences, including allelic or splicevariants, or sequences that encode orthologs or paralogs of presentlydisclosed polypeptides. Nucleic acid hybridization methods are disclosedin detail by Kashima et al. (1985) Nature 313:402-404, and Sambrook etal. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold SpringHarbor Laboratory, Cold Spring Harbor, N.Y. (“Sambrook”); and by Haymeset al., “Nucleic Acid Hybridization: A Practical Approach”, IRL Press,Washington, D.C. (1985), which references are incorporated herein byreference.

In general, stringency is determined by the temperature, ionic strength,and concentration of denaturing agents (e.g., formamide) used in ahybridization and washing procedure (for a more detailed description ofestablishing and determining stringency, see below). The degree to whichtwo nucleic acids hybridize under various conditions of stringency iscorrelated with the extent of their similarity. Thus, similar nucleicacid sequences from a variety of sources, such as within a plant'sgenome (as in the case of paralogs) or from another plant (as in thecase of orthologs) that may perform similar functions can be isolated onthe basis of their ability to hybridize with known transcription factorsequences. Numerous variations are possible in the conditions and meansby which nucleic acid hybridization can be performed to isolatetranscription factor sequences having similarity to transcription factorsequences known in the art and are not limited to those explicitlydisclosed herein. Such an approach may be used to isolate polynucleotidesequences having various degrees of similarity with disclosedtranscription factor sequences, such as, for example, transcriptionfactors having 60% identity, or more preferably greater than about 70%identity, most preferably 72% or greater identity with disclosedtranscription factors.

The term “equivalog” describes members of a set of homologous proteinsthat are conserved with respect to function since their last commonancestor. Related proteins are grouped into equivalog families, andotherwise into protein families with other hierarchically definedhomology types. This definition is provided at the Institute for GenomicResearch (TIGR) website, www.tigr.org; “Terms associated with TIGRFAMs”.

The term “variant”, as used herein, may refer to polynucleotides orpolypeptides that differ from the presently disclosed polynucleotides orpolypeptides, respectively, in sequence from each other, and as setforth below.

With regard to polynucleotide variants, differences between presentlydisclosed polynucleotides and their variants are limited so that thenucleotide sequences of the former and the latter are closely similaroverall and, in many regions, identical. The degeneracy of the geneticcode dictates that many different variant polynucleotides can encodeidentical and/or substantially similar polypeptides in addition to thosesequences illustrated in the Sequence Listing. Due to this degeneracy,differences between presently disclosed polynucleotides and variantnucleotide sequences may be silent in any given region or over theentire length of the polypeptide (i.e., the amino acids encoded by thepolynucleotide are the same, and the variant polynucleotide sequencethus encodes the same amino acid sequence in that region or entirelength of the presently disclosed polynucleotide. Variant nucleotidesequences may encode different amino acid sequences, in which case suchnucleotide differences will result in amino acid substitutions,additions, deletions, insertions, truncations or fusions with respect tothe similar disclosed polynucleotide sequences. These variations resultin polynucleotide variants encoding polypeptides that share at least onefunctional characteristic (i.e., a presently disclosed transcriptionfactor and a variant will confer at least one of the same functions to aplant).

Within the scope of the invention is a variant of a nucleic acid listedin the Sequence Listing (except CBF polynucleotide sequences SEQ ID NOs:1955, 1957, 1959, or 2203), that is, one having a sequence that differsfrom the one of the polynucleotide sequences in the Sequence Listing, ora complementary sequence, that encodes a functionally equivalentpolypeptide (i.e., a polypeptide having some degree of equivalent orsimilar biological activity) but differs in sequence from the sequencein the Sequence Listing, due to degeneracy in the genetic code.

“Allelic variant” or “polynucleotide allelic variant” refers to any oftwo or more alternative forms of a gene occupying the same chromosomallocus. Allelic variation arises naturally through mutation, and mayresult in phenotypic polymorphism within populations. Gene mutations maybe “silent” or may encode polypeptides having altered amino acidsequences. “Allelic variant” and “polypeptide allelic variant” may alsobe used with respect to polypeptides, and in this case the terms referto a polypeptide encoded by an allelic variant of a gene.

“Splice variant” or “polynucleotide splice variant” as used hereinrefers to alternative forms of RNA transcribed from a gene. Splicevariation naturally occurs as a result of alternative sites beingspliced within a single transcribed RNA molecule or between separatelytranscribed RNA molecules, and may result in several different forms ofmRNA transcribed from the same gene. Thus, splice variants may encodepolypeptides having different amino acid sequences, which, in thepresent context, will have at least one similar function in the organism(splice variation may also give rise to distinct polypeptides havingdifferent functions). “Splice variant” or “polypeptide splice variant”may also refer to a polypeptide encoded by a splice variant of atranscribed mRNA.

As used herein, “polynucleotide variants” may also refer topolynucleotide sequences that encode paralogs and orthologs of thepresently disclosed polypeptide sequences. “Polypeptide variants” mayrefer to polypeptide sequences that are paralogs and orthologs of thepresently disclosed polypeptide sequences.

Differences between presently disclosed polypeptides and polypeptidevariants are limited so that the sequences of the former and the latterare closely similar overall and, in many regions, identical. Presentlydisclosed polypeptide sequences and similar polypeptide variants maydiffer in amino acid sequence by one or more substitutions, additions,deletions, fusions and truncations, which may be present in anycombination. These differences may produce silent changes and result ina functionally equivalent transcription factor. Thus, it will be readilyappreciated by those of skill in the art, that any of a variety ofpolynucleotide sequences is capable of encoding the transcriptionfactors and transcription factor homolog polypeptides of the invention.A polypeptide sequence variant may have “conservative” changes, whereina substituted amino acid has similar structural or chemical properties.Deliberate amino acid substitutions may thus be made on the basis ofsimilarity in polarity, charge, solubility, hydrophobicity,hydrophilicity, and/or the amphipathic nature of the residues, as longas the functional or biological activity of the transcription factor isretained. For example, negatively charged amino acids may includeaspartic acid and glutamic acid, positively charged amino acids mayinclude lysine and arginine, and amino acids with uncharged polar headgroups having similar hydrophilicity values may include leucine,isoleucine, and valine; glycine and alanine; asparagine and glutamine;serine and threonine; and phenylalanine and tyrosine. For more detail onconservative substitutions, see Table 2. More rarely, a variant may have“non-conservative” changes, e.g., replacement of a glycine with atryptophan. Similar minor variations may also include amino aciddeletions or insertions, or both. Related polypeptides may comprise, forexample, additions and/or deletions of one or more N-linked or O-linkedglycosylation sites, or an addition and/or a deletion of one or morecysteine residues. Guidance in determining which and how many amino acidresidues may be substituted, inserted or deleted without abolishingfunctional or biological activity may be found using computer programswell known in the art, for example, DNASTAR software (see U.S. Pat. No.5,840,544).

The term “plant” includes whole plants, shoot vegetativeorgans/structures (e.g., leaves, stems and tubers), roots, flowers andfloral organs/structures (e.g., bracts, sepals, petals, stamens,carpels, anthers and ovules), seed (including embryo, endosperm, andseed coat) and fruit (the mature ovary), plant tissue (e.g., vasculartissue, ground tissue, and the like) and cells (e.g., guard cells, eggcells, and the like), and progeny of same. The class of plants that canbe used in the method of the invention is generally as broad as theclass of higher and lower plants amenable to transformation techniques,including angiosperms (monocotyledonous and dicotyledonous plants),gymnosperms, ferns, horsetails, psilophytes, lycophytes, bryophytes, andmulticellular algae. (See for example, FIG. 1, adapted from Daly et al.(2001) Plant Physiol. 127: 1328-1333; FIG. 2, adapted from Ku et al.(2000) Proc. Natl. Acad. Sci. 97: 9121-9126; and see also Tudge, in TheVariety of Life, Oxford University Press, New York, N.Y. (2000) pp.547-606).

A “transgenic plant” refers to a plant that contains genetic materialnot found in a wild-type plant of the same species, variety or cultivar.The genetic material may include a transgene, an insertional mutagenesisevent (such as by transposon or T-DNA insertional mutagenesis), anactivation tagging sequence, a mutated sequence, a homologousrecombination event or a sequence modified by chimeraplasty. Typically,the foreign genetic material has been introduced into the plant by humanmanipulation, but any method can be used as one of skill in the artrecognizes.

A transgenic plant may contain an expression vector or cassette. Theexpression cassette typically comprises a polypeptide-encoding sequenceoperably linked (i.e., under regulatory control of) to appropriateinducible or constitutive regulatory sequences that allow for theexpression of polypeptide. The expression cassette can be introducedinto a plant by transformation or by breeding after transformation of aparent plant. A plant refers to a whole plant, including seedlings andmature plants, as well as to a plant part, such as seed, fruit, leaf, orroot, plant tissue, plant cells or any other plant material, e.g., aplant explant, as well as to progeny thereof, and to in vitro systemsthat mimic biochemical or cellular components or processes in a cell.

“Fragment”, with respect to a polynucleotide, refers to a clone or anypart of a polynucleotide molecule that retains a usable, functionalcharacteristic. Useful fragments include oligonucleotides andpolynucleotides that may be used in hybridization or amplificationtechnologies or in the regulation of replication, transcription ortranslation. A “polynucleotide fragment” refers to any subsequence of apolynucleotide, typically, of at least about 9 consecutive nucleotides,preferably at least about 30 nucleotides, more preferably at least about50 nucleotides, of any of the sequences provided herein. Exemplarypolynucleotide fragments are the first sixty consecutive nucleotides ofthe transcription factor polynucleotides listed in the Sequence Listing.Exemplary fragments also include fragments that comprise a region thatencodes a conserved domain of a transcription factor.

Fragments may also include subsequences of polypeptides and proteinmolecules, or a subsequence of the polypeptide. Fragments may have usesin that they may have antigenic potential. In some cases, the fragmentor domain is a subsequence of the polypeptide that performs at least onebiological function of the intact polypeptide in substantially the samemanner, or to a similar extent, as does the intact polypeptide. Forexample, a polypeptide fragment can comprise a recognizable structuralmotif or functional domain such as a DNA-binding site or domain thatbinds to a DNA promoter region, an activation domain, or a domain forprotein-protein interactions, and may initiate transcription. Fragmentscan vary in size from as few as 3 amino acids to the full length of theintact polypeptide, but are preferably at least about 30 amino acids inlength and more preferably at least about 60 amino acids in length.Exemplary polypeptide fragments are the first twenty consecutive aminoacids of a mammalian protein encoded by are the first twenty consecutiveamino acids of the transcription factor polypeptides listed in theSequence Listing.

Exemplary fragments also include fragments that comprise a conserveddomain of a transcription factor. An example of such an exemplaryfragment would include amino acid residues 59-124 of G867 (SEQ ID NO:170), as noted in Table 5.

The invention also encompasses production of DNA sequences that encodetranscription factors and transcription factor derivatives, or fragmentsthereof, entirely by synthetic chemistry. After production, thesynthetic sequence may be inserted into any of the many availableexpression vectors and cell systems using reagents well known in theart. Moreover, synthetic chemistry may be used to introduce mutationsinto a sequence encoding transcription factors or any fragment thereof.

A “conserved domain” or “conserved region” as used herein refers to aregion in heterologous polynucleotide or polypeptide sequences wherethere is a relatively high degree of sequence identity between thedistinct sequences.

With respect to polynucleotides encoding presently disclosedtranscription factors, a conserved region is preferably at least 10 basepairs (bp) in length.

A “conserved domain”, with respect to presently disclosed polypeptidesrefers to a domain within a transcription factor family that exhibits ahigher degree of sequence homology, such as at least 26% sequencesimilarity, at least 16% sequence identity, preferably at least 40%sequence identity, preferably at least 65% sequence identity includingconservative substitutions, and more preferably at least 80% sequenceidentity, and even more preferably at least 85%, or at least about 86%,or at least about 87%, or at least about 88%, or at least about 90%, orat least about 95%, or at least about 98% amino acid residue sequenceidentity of a polypeptide of consecutive amino acid residues. A fragmentor domain can be referred to as outside a conserved domain, outside aconsensus sequence, or outside a consensus DNA-binding site that isknown to exist or that exists for a particular transcription factorclass, family, or sub-family. In this case, the fragment or domain willnot include the exact amino acids of a consensus sequence or consensusDNA-binding site of a transcription factor class, family or sub-family,or the exact amino acids of a particular transcription factor consensussequence or consensus DNA-binding site. Furthermore, a particularfragment, region, or domain of a polypeptide, or a polynucleotideencoding a polypeptide, can be “outside a conserved domain” if all theamino acids of the fragment, region, or domain fall outside of a definedconserved domain(s) for a polypeptide or protein. Sequences havinglesser degrees of identity but comparable biological activity areconsidered to be equivalents.

As one of ordinary skill in the art recognizes, conserved domains oftranscription factors may be identified as regions or domains ofidentity to a specific consensus sequence (see, for example, Riechmannet al. (2000) supra). Thus, by using alignment methods well known in theart, the conserved domains of the plant transcription factors for eachof the following may be determined: the AP2 (APETALA2) domaintranscription factor family (Riechmann and Meyerowitz (1998) supra; theMYB transcription factor family (ENBib; Martin and Paz-Ares (1997)supra); the MADS domain transcription factor family (Riechmann andMeyerowitz (1997) supra); the WRKY protein family (Ishiguro and Nakamura(1994) supra); the ankyrin-repeat protein family (Zhang et al. (1992)supra); the zinc finger protein (Z) family (Klug and Schwabe (1995)supra; Takatsuji (1998) supra); the homeobox (HB) protein family(Buerglin (1994) supra); the CAAT-element binding proteins (Forsburg andGuarente (1989) supra); the squamosa promoter binding proteins (SPB)(Klein et al. (1996) supra); the NAM protein family (Souer et al. (1996)supra); the IAA/AUX proteins (Abel et al. (1995) supra); the HLH/MYCprotein family (Littlewood et al. (1994) supra); the DNA-binding protein(DBP) family (Tucker et al. (1994) supra); the bZIP family oftranscription factors (Foster et al. (1994) supra); the Box P-bindingprotein (the BPF-1) family (da Costa e Silva et al. (1993) supra); thehigh mobility group (HMG) family (Bustin and Reeves (1996) supra); thescarecrow (SCR) family (Di Laurenzio et al. (1996) supra); the GF14family (Wu et al. (1997) supra); the polycomb (PCOMB) family (Goodrichet al. (1997) supra); the teosinte branched (TEO) family (Luo et al.(1996) supra); the ABI3 family (Giraudat et al. (1992) supra); thetriple helix (TH) family (Dehesh et al. (1990) supra); the EIL family(Chao et al. (1997) Cell supra); the AT-HOOK family (Reeves and Nissen(1990 supra); the S1FA family (Zhou et al. (1995) supra); the bZIPT2family (Lu and Ferl (1995) supra); the YABBY family (Bowman et al.(1999) supra); the PAZ family (Bohmert et al. (1998) supra); a family ofmiscellaneous (MISC) transcription factors including the DPBF family(Kim et al. (1997) supra) and the SPF1 family (Ishiguro and Nakamura(1994) supra); the GARP family (Hall et al. (1998) supra), the TUBBYfamily (Boggin et al. (1999) supra), the heat shock family (Wu (1995supra), the ENBP family (Christiansen et al. (1996) supra), theRING-zinc family (Jensen et al. (1998) supra), the PDBP family (Janik etal. (1989) supra), the PCF family (Cubas et al. (1999) supra), theSRS(SHI-related) family (Fridborg et al. (1999) supra), the CPP(cysteine-rich polycomb-like) family (Cvitanich et al. (2000) supra),the ARF (auxin response factor) family (Ulmasov et al. (1999) supra),the SWI/SNF family (Collingwood et al. (1999) supra), the ACBF family(Seguin et al. (1997) supra), PCGL (CG-1 like) family (da Costa e Silvaet al. (1994) supra) the ARID family (Vazquez et al. (1999) supra), theJumonji family, (Balciunas et al. (2000) supra), the bZIP-NIN family(Schauser et al. (1999) supra), the E2F family Kaelin et al. (1992)supra) and the GRF-like family (Knaap et al (2000) supra).

The conserved domains for each of polypeptides of SEQ ID NO: 2N, whereinN=1-229, are listed in Table 5 as described in Example VII. Also, manyof the polypeptides of Table 5 have conserved domains specificallyindicated by start and stop sites. A comparison of the regions of thepolypeptides in SEQ ID NO: 2N, wherein N=1-229, or of those in Table 5,allows one of skill in the art to identify conserved domain(s) for anyof the polypeptides listed or referred to in this disclosure, includingthose in Tables 4-8.

As used herein, a “gene” is a functional unit of inheritance, and inphysical terms is a particular segment or sequence of nucleotides alonga molecule of DNA (or RNA, in the case of RNA viruses) involved inproducing a functional RNA molecule, such as one used for a structuralor regulatory role, or a polypeptide chain, such as one used for astructural or regulatory role (an example of the latter would betranscription regulation, as by a transcription factor polypeptide).Polypeptides may then be subjected to subsequent processing such assplicing and/or folding to obtain a functional polypeptide. A gene maybe isolated, partially isolated, or be found with an organism's genome.By way of example, a transcription factor gene encodes a transcriptionfactor polypeptide, which may be functional with or without additionalprocessing to function as an initiator of transcription.

Operationally, genes may be defined by the cis-trans test, a genetictest that determines whether two mutations occur in the same gene andwhich may be used to determine the limits of the genetically active unit(Rieger et al. (1976) Glossary of Genetics and Cytogenetics: Classicaland Molecular, 4th ed., Springer Verlag. Berlin). A gene generallyincludes regions preceding (“leaders”; upstream) and following(“trailers”; downstream) of the coding region. A gene may also includeintervening, non-coded sequences, referred to as “introns”, which arelocated between individual coding segments, referred to as “exons”. Mostgenes have an identifiable associated promoter region, a regulatorysequence 5′ or upstream of the transcription initiation codon. Thefunction of a gene may also be regulated by enhancers, operators, andother regulatory elements.

A “trait” refers to a physiological, morphological, biochemical, orphysical characteristic of a plant or particular plant material or cell.In some instances, this characteristic is visible to the human eye, suchas seed or plant size, or can be measured by biochemical techniques,such as detecting the protein, starch, or oil content of seed or leaves,or by observation of a metabolic or physiological process, e.g. bymeasuring uptake of carbon dioxide, or by the observation of theexpression level of a gene or genes, e.g., by employing Northernanalysis, RT-PCR, microarray gene expression assays, or reporter geneexpression systems, or by agricultural observations such as stresstolerance, yield, or pathogen tolerance. Any technique can be used tomeasure the amount of, comparative level of, or difference in anyselected chemical compound or macromolecule in the transgenic plants,however.

“Trait modification” refers to a detectable difference in acharacteristic in a plant ectopically expressing a polynucleotide orpolypeptide of the present invention relative to a plant not doing so,such as a wild-type plant. In some cases, the trait modification can beevaluated quantitatively. For example, the trait modification can entailat least about a 2% increase or decrease in an observed trait(difference), at least a 5% difference, at least about a 10% difference,at least about a 20% difference, at least about a 30%, at least about a50%, at least about a 70%, or at least about a 100%, or an even greaterdifference compared with a wild-type plant. It is known that there canbe a natural variation in the modified trait. Therefore, the traitmodification observed entails a change of the normal distribution of thetrait in the plants compared with the distribution observed in wild-typeplant.

The term “transcript profile” refers to the expression levels of a setof genes in a cell in a particular state, particularly by comparisonwith the expression levels of that same set of genes in a cell of thesame type in a reference state. For example, the transcript profile of aparticular transcription factor in a suspension cell is the expressionlevels of a set of genes in a cell overexpressing that transcriptionfactor compared with the expression levels of that same set of genes ina suspension cell that has normal levels of that transcription factor.The transcript profile can be presented as a list of those genes whoseexpression level is significantly different between the two treatments,and the difference ratios. Differences and similarities betweenexpression levels may also be evaluated and calculated using statisticaland clustering methods.

“Wild type”, as used herein, refers to a cell, tissue or plant that hasnot been genetically modified to knock out or overexpress one or more ofthe presently disclosed transcription factors. Wild-type cells, tissueor plants may be used as controls to compare levels of expression andthe extent and nature of trait modification with modified (e.g.,transgenic) cells, tissue or plants in which transcription factorexpression is altered or ectopically expressed by, for example, knockingout or overexpressing a gene.

“Ectopic expression” or “altered expression” in reference to apolynucleotide indicates that the pattern of expression in, e.g., atransgenic plant or plant tissue, is different from the expressionpattern in a wild-type plant or a reference plant of the same species.The pattern of expression may also be compared with a referenceexpression pattern in a wild-type plant of the same species. Forexample, the polynucleotide or polypeptide is expressed in a cell ortissue type other than a cell or tissue type in which the sequence isexpressed in the wild-type plant, or by expression at a time other thanat the time the sequence is expressed in the wild-type plant, or by aresponse to different inducible agents, such as hormones orenvironmental signals, or at different expression levels (either higheror lower) compared with those found in a wild-type plant. Alteredexpression may be achieved by, for example, transformation of a plantwith an expression cassette having a constitutive or inducible promoterelement associated with a transcription factor gene. The resultingexpression pattern can thus constitutive or inducible, and be stable ortransient. Altered or ectopic expression may also refer to alteredexpression patterns that are produced by lowering the levels ofexpression to below the detection level or completely abolishingexpression by, for example, knocking out a gene's expression bydisrupting expression or regulation of the gene with an insertionelement.

In reference to a polypeptide, the term “ectopic expression or alteredexpression” further may relate to altered activity levels resulting fromthe interactions of the polypeptides with exogenous or endogenousmodulators or from interactions with factors or as a result of thechemical modification of the polypeptides.

The term “overexpression” as used herein refers to a greater expressionlevel of a gene in a plant, plant cell or plant tissue, compared toexpression in a wild-type plant, cell or tissue, at any developmental ortemporal stage for the gene. Overexpression can occur when, for example,the genes encoding one or more transcription factors are under thecontrol of a strong expression signal, such as one of the promotersdescribed herein (e.g., the cauliflower mosaic virus 35S transcriptioninitiation region). Overexpression may occur throughout a plant or inspecific tissues of the plant, depending on the promoter used, asdescribed below.

Overexpression may take place in plant cells normally lacking expressionof polypeptides functionally equivalent or identical to the presenttranscription factors. Overexpression may also occur in plant cellswhere endogenous expression of the present transcription factors orfunctionally equivalent molecules normally occurs, but such normalexpression is at a lower level than in the organism or tissues of theoverexpressor. Overexpression thus results in a greater than normalproduction, or “overproduction” of the transcription factor in theplant, cell or tissue.

The term “phase change” refers to a plant's progression from embryo toadult, and, by some definitions, the transition wherein flowering plantsgain reproductive competency. It is believed that phase change occurseither after a certain number of cell divisions in the shoot apex of adeveloping plant, or when the shoot apex achieves a particular distancefrom the roots. Thus, altering the timing of phase changes may affect aplant's size, which, in turn, may affect yield and biomass.

Traits that May be Modified in Overexpressing or Knock-Out Plants

Trait modifications of particular interest include those to seed (suchas embryo or endosperm), fruit, root, flower, leaf, stem, shoot,seedling or the like, including: enhanced tolerance to environmentalconditions including freezing, chilling, heat, drought, watersaturation, radiation and ozone; improved tolerance to microbial, fungalor viral diseases; improved tolerance to pest infestations, includinginsects, nematodes, mollicutes, parasitic higher plants or the like;decreased herbicide sensitivity; improved tolerance of heavy metals orenhanced ability to take up heavy metals; improved growth under poorphotoconditions (e.g., low light and/or short day length), or changes inexpression levels of genes of interest. Other phenotype that can bemodified relate to the production of plant metabolites, such asvariations in the production of taxol, tocopherol, tocotrienol, sterols,phytosterols, vitamins, wax monomers, anti-oxidants, amino acids,lignins, cellulose, tannins, prenyllipids (such as chlorophylls andcarotenoids), glucosinolates, and terpenoids, enhanced orcompositionally altered protein or oil production (especially in seeds),or modified sugar (insoluble or soluble) and/or starch composition.Physical plant characteristics that can be modified include celldevelopment (such as the number of trichomes), fruit and seed size andnumber, yields of plant parts such as stems, leaves, inflorescences, androots, the stability of the seeds during storage, characteristics of theseed pod (e.g., susceptibility to shattering), root hair length andquantity, internode distances, or the quality of seed coat. Plant growthcharacteristics that can be modified include growth rate, germinationrate of seeds, vigor of plants and seedlings, leaf and flowersenescence, male sterility, apomixis, flowering time, flower abscission,rate of nitrogen uptake, osmotic sensitivity to soluble sugarconcentrations, biomass or transpiration characteristics, as well asplant architecture characteristics such as apical dominance, branchingpatterns, number of organs, organ identity, organ shape or size.

Transcription Factors Modify Expression of Endogenous Genes

Expression of genes that encode transcription factors that modifyexpression of endogenous genes, polynucleotides, and proteins are wellknown in the art. In addition, transgenic plants comprising isolatedpolynucleotides encoding transcription factors may also modifyexpression of endogenous genes, polynucleotides, and proteins. Examplesinclude Peng et al. (1997) Genes and Development 11: 3194-3205, and Penget al. (1999) Nature 400: 256-261. In addition, many others havedemonstrated that an Arabidopsis transcription factor expressed in anexogenous plant species elicits the same or very similar phenotypicresponse. See, for example, Fu et al. (2001) Plant Cell 13: 1791-1802;Nandi et al. (2000, Curr. Biol. 10: 215-218; Coupland (1995) Nature 377:482-483; and Weigel and Nilsson (1995) Nature 377: 482-500.

In another example, Mandel et al. (1992) Cell 71-133-143 and Suzuki etal. (2001) Plant J. 28: 409-418, teach that a transcription factorexpressed in another plant species elicits the same or very similarphenotypic response of the endogenous sequence, as often predicted inearlier studies of Arabidopsis transcription factors in Arabidopsis (seeMandel et al. (1992) supra; Suzuki et al. (2001) supra).

Other examples include Müller et al. (2001) Plant J. 28: 169-179; Kim etal. (2001) Plant J. 25: 247-259; Kyozuka and Shimamoto (2002) Plant CellPhysiol. 43: 130-135; Boss and Thomas (2002) Nature 416: 847-850; He etal. (2000) Transgenic Res. 9: 223-227; and Robson et al. (2001) Plant J.28: 619-631.

In yet another example, Gilmour et al. (1998) Plant J. 16: 433-442,teach an Arabidopsis AP2 transcription factor, CBF1 (SEQ ID NO: 1956),which, when overexpressed in transgenic plants, increases plant freezingtolerance. Jaglo et al. (2001) Plant Physiol. 127: 910-917, furtheridentified sequences in Brassica napus which encode CBF-like genes andthat transcripts for these genes accumulated rapidly in response to lowtemperature. Transcripts encoding CBF-like proteins were also found toaccumulate rapidly in response to low temperature in wheat, as well asin tomato. An alignment of the CBF proteins from Arabidopsis, B. napus,wheat, rye, and tomato revealed the presence of conserved consecutiveamino acid residues, PKK/RPAGRxKFxETRHP (SEQ ID NO: 2907) and DSAWR (SEQID NO: 2908), that bracket the AP2/EREBP DNA binding domains of theproteins and distinguish them from other members of the AP2/EREBPprotein family (See Jaglo et al. supra).

Gao et al. (2002) Plant Molec. Biol. 49: 459-471) have recentlydescribed four CBF transcription factors from Brassica napus: BNCBFs 5,7, 16 and 17. They note that the first three CBFs (GenBank AccessionNumbers AAM18958, AAM18959, and AAM18960, respectively) are very similarto Arabidopsis CBF1, whereas BNCBF17 (GenBank Accession Number AAM18961)is similar but contains two extra regions of 16 and 21 amino acids inits acidic activation domain. All four B. napus CBFs accumulate inleaves of the plants after cold-treatment, and BNCBFs 5, 7, 16accumulated after salt stress treatment. The authors concluded thatthese BNCBFs likely function in low-temperature responses in B. napus.

In a functional study of CBF genes, Hsieh et al. ((2002) Plant Physiol.129: 1086-1094) found that heterologous expression of Arabidopsis CBF1in tomato plants confers increased tolerance to chilling andconsiderable tolerance to oxidative stress, which suggested to theauthors that ectopic Arabidopsis CBF1 expression may induce severaltomato stress responsive genes to protect the plants.

Polypeptides and Polynucleotides of the Invention

The present invention provides, among other things, transcriptionfactors (TFs), and transcription factor homolog polypeptides, andisolated or recombinant polynucleotides encoding the polypeptides, ornovel sequence variant polypeptides or polynucleotides encoding novelvariants of transcription factors derived from the specific sequencesprovided here. These polypeptides and polynucleotides may be employed tomodify a plant's characteristics.

Exemplary polynucleotides encoding the polypeptides of the inventionwere identified in the Arabidopsis thaliana GenBank database usingpublicly available sequence analysis programs and parameters. Sequencesinitially identified were then further characterized to identifysequences comprising specified sequence strings corresponding tosequence motifs present in families of known transcription factors. Inaddition, further exemplary polynucleotides encoding the polypeptides ofthe invention were identified in the plant GenBank database usingpublicly available sequence analysis programs and parameters. Sequencesinitially identified were then further characterized to identifysequences comprising specified sequence strings corresponding tosequence motifs present in families of known transcription factors.Polynucleotide sequences meeting such criteria were confirmed astranscription factors.

Additional polynucleotides of the invention were identified by screeningArabidopsis thaliana and/or other plant cDNA libraries with probescorresponding to known transcription factors under low stringencyhybridization conditions. Additional sequences, including full lengthcoding sequences were subsequently recovered by the rapid amplificationof cDNA ends (RACE) procedure, using a commercially available kitaccording to the manufacturer's instructions. Where necessary, multiplerounds of RACE are performed to isolate 5′ and 3′ ends. The full-lengthcDNA was then recovered by a routine end-to-end polymerase chainreaction (PCR) using primers specific to the isolated 5′ and 3′ ends.Exemplary sequences are provided in the Sequence Listing.

The polynucleotides of the invention can be or were ectopicallyexpressed in overexpressor or knockout plants and the changes in thecharacteristic(s) or trait(s) of the plants observed. Therefore, thepolynucleotides and polypeptides can be employed to improve thecharacteristics of plants.

The polynucleotides of the invention can be or were ectopicallyexpressed in overexpressor plant cells and the changes in the expressionlevels of a number of genes, polynucleotides, and/or proteins of theplant cells observed. Therefore, the polynucleotides and polypeptidescan be employed to change expression levels of a genes, polynucleotides,and/or proteins of plants.

Producing Polypeptides

The polynucleotides of the invention include sequences that encodetranscription factors and transcription factor homolog polypeptides andsequences complementary thereto, as well as unique fragments of codingsequence, or sequence complementary thereto. Such polynucleotides canbe, e.g., DNA or RNA, e.g., mRNA, cRNA, synthetic RNA, genomic DNA, cDNAsynthetic DNA, oligonucleotides, etc. The polynucleotides are eitherdouble-stranded or single-stranded, and include either, or both sense(i.e., coding) sequences and antisense (i.e., non-coding, complementary)sequences. The polynucleotides include the coding sequence of atranscription factor, or transcription factor homolog polypeptide, inisolation, in combination with additional coding sequences (e.g., apurification tag, a localization signal, as a fusion-protein, as apre-protein, or the like), in combination with non-coding sequences(e.g., introns or inteins, regulatory elements such as promoters,enhancers, terminators, and the like), and/or in a vector or hostenvironment in which the polynucleotide encoding a transcription factoror transcription factor homolog polypeptide is an endogenous orexogenous gene.

A variety of methods exist for producing the polynucleotides of theinvention. Procedures for identifying and isolating DNA clones are wellknown to those of skill in the art, and are described in, e.g., Bergerand Kimmel, Guide to Molecular Cloning Techniques, Methods inEnzymology, vol. 152 Academic Press, Inc., San Diego, Calif. (“Berger”);Sambrook et al. (1989) Molecular Cloning—A Laboratory Manual (2nd Ed.),Vol. 1-3, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., andCurrent Protocols in Molecular Biology, Ausubel et al. eds., CurrentProtocols, a joint venture between Greene Publishing Associates, Inc.and John Wiley & Sons, Inc., (supplemented through 2000) (“Ausubel”).

Alternatively, polynucleotides of the invention, can be produced by avariety of in vitro amplification methods adapted to the presentinvention by appropriate selection of specific or degenerate primers.Examples of protocols sufficient to direct persons of skill through invitro amplification methods, including the polymerase chain reaction(PCR) the ligase chain reaction (LCR), Qbeta-replicase amplification andother RNA polymerase mediated techniques (e.g., NASBA), e.g., for theproduction of the homologous nucleic acids of the invention are found inBerger (supra), Sambrook (supra), and Ausubel (supra), as well as Mulliset al. (1987) PCR Protocols A Guide to Methods and Applications (Inniset al. eds) Academic Press Inc. San Diego, Calif. (1990) (Innis)Improved methods for cloning in vitro amplified nucleic acids aredescribed in Wallace et al. U.S. Pat. No. 5,426,039. Improved methodsfor amplifying large nucleic acids by PCR are summarized in Cheng et al.(1994) Nature 369: 684-685 and the references cited therein, in whichPCR amplicons of up to 40 kb are generated. One of skill will appreciatethat essentially any RNA can be converted into a double stranded DNAsuitable for restriction digestion, PCR expansion and sequencing usingreverse transcriptase and a polymerase. See, e.g., Ausubel, Sambrook andBerger, all supra.

Alternatively, polynucleotides and oligonucleotides of the invention canbe assembled from fragments produced by solid-phase synthesis methods.Typically, fragments of up to approximately 100 bases are individuallysynthesized and then enzymatically or chemically ligated to produce adesired sequence, e.g., a polynucleotide encoding all or part of atranscription factor. For example, chemical synthesis using thephosphoramidite method is described, e.g., by Beaucage et al. (1981)Tetrahedron Letters 22: 1859-1869; and Matthes et al. (1984) EMBO J. 3:801-805. According to such methods, oligonucleotides are synthesized,purified, annealed to their complementary strand, ligated and thenoptionally cloned into suitable vectors. And if so desired, thepolynucleotides and polypeptides of the invention can be custom orderedfrom any of a number of commercial suppliers.

Homologous Sequences

Sequences homologous, i.e., that share significant sequence identity orsimilarity, to those provided in the Sequence Listing (except CBFsequences SEQ ID NOs: 1955-1960), derived from Arabidopsis thaliana orfrom other plants of choice, are also an aspect of the invention.Homologous sequences can be derived from any plant including monocotsand dicots and in particular agriculturally important plant species,including but not limited to, crops such as soybean, wheat, corn(maize), potato, cotton, rice, rape, oilseed rape (including canola),sunflower, alfalfa, clover, sugarcane, and turf; or fruits andvegetables, such as banana, blackberry, blueberry, strawberry, andraspberry, cantaloupe, carrot, cauliflower, coffee, cucumber, eggplant,grapes, honeydew, lettuce, mango, melon, onion, papaya, peas, peppers,pineapple, pumpkin, spinach, squash, sweet corn, tobacco, tomato,tomatillo, watermelon, rosaceous fruits (such as apple, peach, pear,cherry and plum) and vegetable brassicas (such as broccoli, cabbage,cauliflower, Brussels sprouts, and kohlrabi). Other crops, includingfruits and vegetables, whose phenotype can be changed and which comprisehomologous sequences include barley; rye; millet; sorghum; currant;avocado; citrus fruits such as oranges, lemons, grapefruit andtangerines, artichoke, cherries; nuts such as the walnut and peanut;endive; leek; roots such as arrowroot, beet, cassava, turnip, radish,yam, and sweet potato; and beans. The homologous sequences may also bederived from woody species, such pine, poplar and eucalyptus, or mint orother labiates. In addition, homologous sequences may be derived fromplants that are evolutionarily-related to crop plants, but which may nothave yet been used as crop plants. Examples include deadly nightshade(Atropa belladona), related to tomato; jimson weed (Datura strommium),related to peyote; and teosinte (Zea species), related to corn (maize)

Orthologs and Paralogs

Homologous sequences as described above can comprise orthologous orparalogous sequences. Several different methods are known by those ofskill in the art for identifying and defining these functionallyhomologous sequences. Three general methods for defining orthologs andparalogs are described; an ortholog or paralog, including equivalogs,may be identified by one or more of the methods described below.

Orthologs and paralogs are evolutionarily related genes that havesimilar sequence and similar functions. Orthologs are structurallyrelated genes in different species that are derived by a speciationevent. Paralogs are structurally related genes within a single speciesthat are derived by a duplication event.

Within a single plant species, gene duplication may cause two copies ofa particular gene, giving rise to two or more genes with similarsequence and often similar function known as paralogs. A paralog istherefore a similar gene formed by duplication within the same species.Paralogs typically cluster together or in the same clade (a group ofsimilar genes) when a gene family phylogeny is analyzed using programssuch as CLUSTAL (Thompson et al. (1994) Nucleic Acids Res. 22:4673-4680; Higgins et al. (1996) Methods Enzymol. 266: 383-402). Groupsof similar genes can also be identified with pair-wise BLAST analysis(Feng and Doolittle (1987) J. Mol. Evol. 25: 351-360). For example, aclade of very similar MADS domain transcription factors from Arabidopsisall share a common function in flowering time (Ratcliffe et al. (2001)Plant Physiol. 126: 122-132), and a group of very similar AP2 domaintranscription factors from Arabidopsis are involved in tolerance ofplants to freezing (Gilmour et al. (1998) Plant J. 16: 433-442).Analysis of groups of similar genes with similar function that fallwithin one clade can yield sub-sequences that are particular to theclade. These sub-sequences, known as consensus sequences, can not onlybe used to define the sequences within each clade, but define thefunctions of these genes; genes within a clade may contain paralogoussequences, or orthologous sequences that share the same function (seealso, for example, Mount (2001), in Bioinformatics: Sequence and GenomeAnalysis, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.,page 543.)

Speciation, the production of new species from a parental species, canalso give rise to two or more genes with similar sequence and similarfunction. These genes, termed orthologs, often have an identicalfunction within their host plants and are often interchangeable betweenspecies without losing function. Because plants have common ancestors,many genes in any plant species will have a corresponding orthologousgene in another plant species. Once a phylogenic tree for a gene familyof one species has been constructed using a program such as CLUSTAL(Thompson et al. (1994) Nucleic Acids Res. 22: 4673-4680; Higgins et al.(1996) supra) potential orthologous sequences can be placed into thephylogenetic tree and their relationship to genes from the species ofinterest can be determined. Orthologous sequences can also be identifiedby a reciprocal BLAST strategy. Once an orthologous sequence has beenidentified, the function of the ortholog can be deduced from theidentified function of the reference sequence.

Transcription factor gene sequences are conserved across diverseeukaryotic species lines (Goodrich et al. (1993) Cell 75: 519-530; Linet al. (1991) Nature 353: 569-571; Sadowski et al. (1988) Nature 335:563-564). et al. Plants are no exception to this observation; diverseplant species possess transcription factors that have similar sequencesand functions.

Orthologous genes from different organisms have highly conservedfunctions, and very often essentially identical functions (Lee et al.(2002) Genome Res. 12: 493-502; Remm et al. (2001) J. Mol. Biol. 314:1041-1052). Paralogous genes, which have diverged through geneduplication, may retain similar functions of the encoded proteins. Insuch cases, paralogs can be used interchangeably with respect to certainembodiments of the instant invention (for example, transgenic expressionof a coding sequence). An example of such highly related paralogs is theCBF family, with three well-defined members in Arabidopsis and at leastone ortholog in Brassica napus (SEQ ID NOs: 1956, 1958, 1960, or 2204,respectively), all of which control pathways involved in both freezingand drought stress (Gilmour et al. (1998) Plant J. 16: 433-442; Jaglo etal. (1998) Plant Physiol. 127: 910-917).

The following references represent a small sampling of the many studiesthat demonstrate that conserved transcription factor genes from diversespecies are likely to function similarly (i.e., regulate similar targetsequences and control the same traits), and that transcription factorsmay be transformed into diverse species to confer or improve traits.

(1) The Arabidopsis NPR1 gene regulates systemic acquired resistance(SAR); over-expression of NPR1 leads to enhanced resistance inArabidopsis. When either Arabidopsis NPR1 or the rice NPR1 ortholog wasoverexpressed in rice (which, as a monocot, is diverse fromArabidopsis), challenge with the rice bacterial blight pathogenXanthomonas oryzae pv. Oryzae, the transgenic plants displayed enhancedresistance (Chern et al. (2001) Plant J. 27: 101-113). NPR1 acts throughactivation of expression of transcription factor genes, such as TGA2(Fan and Dong (2002) Plant Cell 14: 1377-1389).

(2) E2F genes are involved in transcription of plant genes forproliferating cell nuclear antigen (PCNA). Plant E2Fs share a highdegree of similarity in amino acid sequence between monocots and dicots,and are even similar to the conserved domains of the animal E2Fs. Suchconservation indicates a functional similarity between plant and animalE2Fs. E2F transcription factors that regulate meristem development actthrough common cis-elements, and regulate related (PCNA) genes (Kosugiand Ohashi, (2002) Plant J. 29: 45-59).

(3) The ABI5 gene (abscisic acid (ABA) insensitive 5) encodes a basicleucine zipper factor required for ABA response in the seed andvegetative tissues. Co-transformation experiments with ABI5 cDNAconstructs in rice protoplasts resulted in specific transactivation ofthe ABA-inducible wheat, Arabidopsis, bean, and barley promoters. Theseresults demonstrate that sequentially similar ABI5 transcription factorsare key targets of a conserved ABA signaling pathway in diverse plants.(Gampala et al. (2001) J. Biol. Chem. 277: 1689-1694).

(4) Sequences of three Arabidopsis GAMYB-like genes were obtained on thebasis of sequence similarity to GAMYB genes from barley, rice, and L.temulentum. These three Arabadopsis genes were determined to encodetranscription factors (AtMYB33, AtMYB65, and AtMYB101) and couldsubstitute for a barley GAMYB and control alpha-amylase expression(Gocal et al. (2001) Plant Physiol. 127: 1682-1693).

(5) The floral control gene LEAFY from Arabidopsis can dramaticallyaccelerate flowering in numerous dictoyledonous plants. Constitutiveexpression of Arabidopsis LEAFY also caused early flowering intransgenic rice (a monocot), with a heading date that was 26-34 daysearlier than that of wild-type plants. These observations indicate thatfloral regulatory genes from Arabidopsis are useful tools for headingdate improvement in cereal crops (He et al. (2000) Transgenic Res. 9:223-227).

(6) Bioactive gibberellins (GAs) are essential endogenous regulators ofplant growth. GA signaling tends to be conserved across the plantkingdom. GA signaling is mediated via GAL a nuclear member of the GRASfamily of plant transcription factors. Arabidopsis GAI has been shown tofunction in rice to inhibit gibberellin response pathways (Fu et al.(2001) Plant Cell 13: 1791-1802).

(7) The Arabidopsis gene SUPERMAN (SUP), encodes a putativetranscription factor that maintains the boundary between stamens andcarpels. By over-expressing Arabidopsis SUP in rice, the effect of thegene's presence on whorl boundaries was shown to be conserved. Thisdemonstrated that SUP is a conserved regulator of floral whorlboundaries and affects cell proliferation (Nandi et al. (2000) Curr.Biol. 10: 215-218).

(8) Maize, petunia and Arabidopsis myb transcription factors thatregulate flavonoid biosynthesis are very genetically similar and affectthe same trait in their native species, therefore sequence and functionof these myb transcription factors correlate with each other in thesediverse species (Borevitz et al. (2000) Plant Cell 12: 2383-2394).

(9) Wheat reduced height-1 (Rht-B1/Rht-D1) and maize dwarf-8 (d8) genesare orthologs of the Arabidopsis gibberellin insensitive (GAI) gene.Both of these genes have been used to produce dwarf grain varieties thathave improved grain yield. These genes encode proteins that resemblenuclear transcription factors and contain an SH2-like domain, indicatingthat phosphotyrosine may participate in gibberellin signaling.Transgenic rice plants containing a mutant GAI allele from Arabidopsishave been shown to produce reduced responses to gibberellin and aredwarfed, indicating that mutant GAI orthologs could be used to increaseyield in a wide range of crop species (Peng et al. (1999) Nature 400:256-261).

Transcription factors that are homologous to the listed sequences willtypically share, in at least one conserved domain, at least about 70%amino acid sequence identity, and with regard to zinc fingertranscription factors, at least about 50% amino acid sequence identity.More closely related transcription factors can share at least about 70%,or about 75% or about 80% or about 90% or about 95% or about 98% or moresequence identity with the listed sequences, or with the listedsequences but excluding or outside a known consensus sequence orconsensus DNA-binding site, or with the listed sequences excluding oneor all conserved domain. Factors that are most closely related to thelisted sequences share, e.g., at least about 85%, about 90% or about 95%or more % sequence identity to the listed sequences, or to the listedsequences but excluding or outside a known consensus sequence orconsensus DNA-binding site or outside one or all conserved domain. Atthe nucleotide level, the sequences will typically share at least about40% nucleotide sequence identity, preferably at least about 50%, about60%, about 70% or about 80% sequence identity, and more preferably about85%, about 90%, about 95% or about 97% or more sequence identity to oneor more of the listed sequences, or to a listed sequence but excludingor outside a known consensus sequence or consensus DNA-binding site, oroutside one or all conserved domain. The degeneracy of the genetic codeenables major variations in the nucleotide sequence of a polynucleotidewhile maintaining the amino acid sequence of the encoded protein.Conserved domains within a transcription factor family may exhibit ahigher degree of sequence homology, such as at least 65% amino acidsequence identity including conservative substitutions, and preferablyat least 80% sequence identity, and more preferably at least 85%, or atleast about 86%, or at least about 87%, or at least about 88%, or atleast about 90%, or at least about 95%, or at least about 98% sequenceidentity. Transcription factors that are homologous to the listedsequences should share at least 30%, or at least about 60%, or at leastabout 75%, or at least about 80%, or at least about 90%, or at leastabout 95% amino acid sequence identity over the entire length of thepolypeptide or the homolog.

Percent identity can be determined electronically, e.g., by using theMEGALIGN program (DNASTAR, Inc. Madison, Wis.). The MEGALIGN program cancreate alignments between two or more sequences according to differentmethods, for example, the clustal method. (See, for example, Higgins andSharp (1988) Gene 73: 237-244.) The clustal algorithm groups sequencesinto clusters by examining the distances between all pairs. The clustersare aligned pairwise and then in groups. Other alignment algorithms orprograms may be used, including FASTA, BLAST, or ENTREZ, FASTA andBLAST, and which may be used to calculate percent similarity. These areavailable as a part of the GCG sequence analysis package (University ofWisconsin, Madison, Wis.), and can be used with or without defaultsettings. ENTREZ is available through the National Center forBiotechnology Information. In one embodiment, the percent identity oftwo sequences can be determined by the GCG program with a gap weight of1, e.g., each amino acid gap is weighted as if it were a single aminoacid or nucleotide mismatch between the two sequences (see U.S. Pat. No.6,262,333).

Other techniques for alignment are described in Doolittle, R. F. (1996)Methods in Enzymology: Computer Methods for Macromolecular SequenceAnalysis, vol. 266, Academic Press, Orlando, Fla., USA. Preferably, analignment program that permits gaps in the sequence is utilized to alignthe sequences. The Smith-Waterman is one type of algorithm that permitsgaps in sequence alignments (see Shpaer (1997) Methods Mol. Biol. 70:173-187). Also, the GAP program using the Needleman and Wunsch alignmentmethod can be utilized to align sequences. An alternative searchstrategy uses MPSRCH software, which runs on a MASPAR computer. MPSRCHuses a Smith-Waterman algorithm to score sequences on a massivelyparallel computer. This approach improves ability to pick up distantlyrelated matches, and is especially tolerant of small gaps and nucleotidesequence errors. Nucleic acid-encoded amino acid sequences can be usedto search both protein and DNA databases.

The percentage similarity between two polypeptide sequences, e.g.,sequence A and sequence B, is calculated by dividing the length ofsequence A, minus the number of gap residues in sequence A, minus thenumber of gap residues in sequence B, into the sum of the residuematches between sequence A and sequence B, times one hundred. Gaps oflow or of no similarity between the two amino acid sequences are notincluded in determining percentage similarity. Percent identity betweenpolynucleotide sequences can also be counted or calculated by othermethods known in the art, e.g., the Jotun Hein method. (See, e.g., Hein(1990) Methods Enzymol. 183: 626-645.) Identity between sequences canalso be determined by other methods known in the art, e.g., by varyinghybridization conditions (see US Patent Application No. 20010010913).

The percent identity between two conserved domains of a transcriptionfactor DNA-binding domain consensus polypeptide sequence can be as lowas 16%, as exemplified in the case of GATA1 family of eukaryoticCys₂/Cys₂-type zinc finger transcription factors. The DNA-binding domainconsensus polypeptide sequence of the GATA1 family is CX₂CX₁₇CX₂C, whereX is any amino acid residue. (See, for example, Takatsuji, supra.) Otherexamples of such conserved consensus polypeptide sequences with lowoverall percent sequence identity are well known to those of skill inthe art.

Thus, the invention provides methods for identifying a sequence similaror paralogous or orthologous or homologous to one or morepolynucleotides as noted herein, or one or more target polypeptidesencoded by the polynucleotides, or otherwise noted herein and mayinclude linking or associating a given plant phenotype or gene functionwith a sequence. In the methods, a sequence database is provided(locally or across an internet or intranet) and a query is made againstthe sequence database using the relevant sequences herein and associatedplant phenotypes or gene functions.

In addition, one or more polynucleotide sequences or one or morepolypeptides encoded by the polynucleotide sequences may be used tosearch against a BLOCKS (Bairoch et al. (1997) Nucleic Acids Res. 25:217-221), PFAM, and other databases which contain previously identifiedand annotated motifs, sequences and gene functions. Methods that searchfor primary sequence patterns with secondary structure gap penalties(Smith et al. (1992) Protein Engineering 5: 35-51) as well as algorithmssuch as Basic Local Alignment Search Tool (BLAST; Altschul (1993) J.Mol. Evol. 36: 290-300; Altschul et al. (1990) supra), BLOCKS (Henikoffand Henikoff (1991) Nucleic Acids Res. 19: 6565-6572), Hidden MarkovModels (HMM; Eddy (1996) Curr. Opin. Str. Biol. 6: 361-365; Sonnhammeret al. (1997) Proteins 28: 405-420), and the like, can be used tomanipulate and analyze polynucleotide and polypeptide sequences encodedby polynucleotides. These databases, algorithms and other methods arewell known in the art and are described in Ausubel et al. (1997; ShortProtocols in Molecular Biology, John Wiley & Sons, New York, N.Y., unit7.7) and in Meyers (1995; Molecular Biology and Biotechnology, WileyVCH, New York, N.Y., p 856-853).

Furthermore, methods using manual alignment of sequences similar orhomologous to one or more polynucleotide sequences or one or morepolypeptides encoded by the polynucleotide sequences may be used toidentify regions of similarity and conserved domains. Such manualmethods are well-known of those of skill in the art and can include, forexample, comparisons of tertiary structure between a polypeptidesequence encoded by a polynucleotide which comprises a known functionwith a polypeptide sequence encoded by a polynucleotide sequence whichhas a function not yet determined. Such examples of tertiary structuremay comprise predicted alpha helices, beta-sheets, amphipathic helices,leucine zipper motifs, zinc finger motifs, proline-rich regions,cysteine repeat motifs, and the like.

Orthologs and paralogs of presently disclosed transcription factors maybe cloned using compositions provided by the present invention accordingto methods well known in the art. cDNAs can be cloned using mRNA from aplant cell or tissue that expresses one of the present transcriptionfactors. Appropriate mRNA sources may be identified by interrogatingNorthern blots with probes designed from the present transcriptionfactor sequences, after which a library is prepared from the mRNAobtained from a positive cell or tissue. Transcription factor-encodingcDNA is then isolated using, for example, PCR, using primers designedfrom a presently disclosed transcription factor gene sequence, or byprobing with a partial or complete cDNA or with one or more sets ofdegenerate probes based on the disclosed sequences. The cDNA library maybe used to transform plant cells. Expression of the cDNAs of interest isdetected using, for example, methods disclosed herein such asmicroarrays, Northern blots, quantitative PCR, or any other techniquefor monitoring changes in expression. Genomic clones may be isolatedusing similar techniques to those.

Identifying Polynucleotides or Nucleic Acids by Hybridization

Polynucleotides homologous to the sequences illustrated in the SequenceListing and tables can be identified, e.g., by hybridization to eachother under stringent or under highly stringent conditions. Singlestranded polynucleotides hybridize when they associate based on avariety of well characterized physical-chemical forces, such as hydrogenbonding, solvent exclusion, base stacking and the like. The stringencyof a hybridization reflects the degree of sequence identity of thenucleic acids involved, such that the higher the stringency, the moresimilar are the two polynucleotide strands. Stringency is influenced bya variety of factors, including temperature, salt concentration andcomposition, organic and non-organic additives, solvents, etc. presentin both the hybridization and wash solutions and incubations (and numberthereof), as described in more detail in the references cited above.

Encompassed by the invention are polynucleotide sequences that arecapable of hybridizing to the claimed polynucleotide sequences,including any of the transcription factor polynucleotides within theSequence Listing (excluding CBF sequences SEQ ID NOs: 1955, 1957, 1959,or 2203), and fragments thereof under various conditions of stringency(See, for example, Wahl and Berger (1987) Methods Enzymol. 152: 399-407;and Kimmel (1987) Methods Enzymol. 152: 507-511). In addition to thenucleotide sequences listed in Tables 4 and 5, full length cDNA,orthologs, and paralogs of the present nucleotide sequences may beidentified and isolated using well-known methods. The cDNA librariesorthologs, and paralogs of the present nucleotide sequences may bescreened using hybridization methods to determine their utility ashybridization target or amplification probes.

With regard to hybridization, conditions that are highly stringent, andmeans for achieving them, are well known in the art. See, for example,Sambrook et al. (1989) “Molecular Cloning: A Laboratory Manual” (2nded., Cold Spring Harbor Laboratory); Berger and Kimmel, eds., (1987)“Guide to Molecular Cloning Techniques”, In Methods in Enzymology: 152:467-469; and Anderson and Young (1985) “Quantitative FilterHybridisation.” In: Hames and Higgins, ed., Nucleic Acid Hybridisation,A Practical Approach. Oxford, IRL Press, 73-111.

Stability of DNA duplexes is affected by such factors as basecomposition, length, and degree of base pair mismatch. Hybridizationconditions may be adjusted to allow DNAs of different sequencerelatedness to hybridize. The melting temperature (T_(m)) is defined asthe temperature when 50% of the duplex molecules have dissociated intotheir constituent single strands. The melting temperature of a perfectlymatched duplex, where the hybridization buffer contains formamide as adenaturing agent, may be estimated by the following equation:DNA-DNA: T _(m)(° C.)=81.5+16.6(log [Na+])+0.41(% G+C)−0.62(%formamide)−500/L  (1)DNA-RNA: T _(m)(° C.)=79.8+18.5(log [Na+])+0.58(% G+C)+0.12(%G+C)²−0.5(% formamide)−820/L  (2)RNA-RNA: T _(m)(° C.)=79.8+18.5(log [Na+])+0.58(% G+C)+0.12(%G+C)²−0.35(% formamide)−820/L  (3)

where L is the length of the duplex formed, [Na+] is the molarconcentration of the sodium ion in the hybridization or washingsolution, and % G+C is the percentage of (guanine+cytosine) bases in thehybrid. For imperfectly matched hybrids, approximately 1° C. is requiredto reduce the melting temperature for each 1-% mismatch.

Hybridization experiments are generally conducted in a buffer of pHbetween 6.8 to 7.4, although the rate of hybridization is nearlyindependent of pH at ionic strengths likely to be used in thehybridization buffer (Anderson et al. (1985) supra). In addition, one ormore of the following may be used to reduce non-specific hybridization:sonicated salmon sperm DNA or another non-complementary DNA, bovineserum albumin, sodium pyrophosphate, sodium dodecylsulfate (SDS),polyvinyl-pyrrolidone, ficoll and Denhardt's solution. Dextran sulfateand polyethylene glycol 6000 act to exclude DNA from solution, thusraising the effective probe DNA concentration and the hybridizationsignal within a given unit of time. In some instances, conditions ofeven greater stringency may be desirable or required to reducenon-specific and/or background hybridization. These conditions may becreated with the use of higher temperature, lower ionic strength andhigher concentration of a denaturing agent such as formamide.

Stringency conditions can be adjusted to screen for moderately similarfragments such as homologous sequences from distantly related organisms,or to highly similar fragments such as genes that duplicate functionalenzymes from closely related organisms. The stringency can be adjustedeither during the hybridization step or in the post-hybridizationwashes. Salt concentration, formamide concentration, hybridizationtemperature and probe lengths are variables that can be used to alterstringency (as described by the formula above). As a general guidelineshigh stringency is typically performed at T_(m)−5° C. to T_(m)−20° C.,moderate stringency at T_(m)−20° C. to T_(m)−35° C. and low stringencyat T_(m)−35° C. to T_(m)−50° C. for duplex>150 base pairs. Hybridizationmay be performed at low to moderate stringency (25-50° C. below T_(m)),followed by post-hybridization washes at increasing stringencies.Maximum rates of hybridization in solution are determined empirically tooccur at T_(m)−25° C. for DNA-DNA duplex and T_(m)−15° C. for RNA-DNAduplex. Optionally, the degree of dissociation may be assessed aftereach wash step to determine the need for subsequent, higher stringencywash steps.

High stringency conditions may be used to select for nucleic acidsequences with high degrees of identity to the disclosed sequences. Anexample of stringent hybridization conditions obtained in a filter-basedmethod such as a Southern or northern blot for hybridization ofcomplementary nucleic acids that have more than 100 complementaryresidues is about 5° C. to 20° C. lower than the thermal melting point(T_(m)) for the specific sequence at a defined ionic strength and pH.Conditions used for hybridization may include about 0.02 M to about 0.15M sodium chloride, about 0.5% to about 5% casein, about 0.02% SDS orabout 0.1% N-laurylsarcosine, about 0.001 M to about 0.03 M sodiumcitrate, at hybridization temperatures between about 50° C. and about70° C. More preferably, high stringency conditions are about 0.02 Msodium chloride, about 0.5% casein, about 0.02% SDS, about 0.001 Msodium citrate, at a temperature of about 50° C. Nucleic acid moleculesthat hybridize under stringent conditions will typically hybridize to aprobe based on either the entire DNA molecule or selected portions,e.g., to a unique subsequence, of the DNA.

Stringent salt concentration will ordinarily be less than about 750 mMNaCl and 75 mM trisodium citrate. Increasingly stringent conditions maybe obtained with less than about 500 mM NaCl and 50 mM trisodiumcitrate, to even greater stringency with less than about 250 mM NaCl and25 mM trisodium citrate. Low stringency hybridization can be obtained inthe absence of organic solvent, e.g., formamide, whereas high stringencyhybridization may be obtained in the presence of at least about 35%formamide, and more preferably at least about 50% formamide. Stringenttemperature conditions will ordinarily include temperatures of at leastabout 30° C., more preferably of at least about 37° C., and mostpreferably of at least about 42° C. with formamide present. Varyingadditional parameters, such as hybridization time, the concentration ofdetergent, e.g., sodium dodecyl sulfate (SDS) and ionic strength, arewell known to those skilled in the art. Various levels of stringency areaccomplished by combining these various conditions as needed. In apreferred embodiment, hybridization will occur at 30° C. in 750 mM NaCl,75 mM trisodium citrate, and 1% SDS. In a more preferred embodiment,hybridization will occur at 37° C. in 500 mM NaCl, 50 mM trisodiumcitrate, 1% SDS, 35% formamide. In a most preferred embodiment,hybridization will occur at 42° C. in 250 mM NaCl, 25 mM trisodiumcitrate, 1% SDS, 50% formamide. Useful variations on these conditionswill be readily apparent to those skilled in the art.

The washing steps that follow hybridization may also vary in stringency;the post-hybridization wash steps primarily determine hybridizationspecificity, with the most critical factors being temperature and theionic strength of the final wash solution. Wash stringency can beincreased by decreasing salt concentration or by increasing temperature.Stringent salt concentration for the wash steps will preferably be lessthan about 30 mM NaCl and 3 mM trisodium citrate, and most preferablyless than about 15 mM NaCl and 1.5 mM trisodium citrate. For example,the wash conditions may be under conditions of 0.1×SSC to 2.0×SSC and0.1% SDS at 50-65° C., with, for example, two steps of 10-30 min. Oneexample of stringent wash conditions includes about 2.0×SSC, 0.1% SDS at65° C. and washing twice, each wash step being about 30 min. A higherstringency wash is about 0.2×SSC, 0.1% SDS at 65° C. and washing twicefor 30 min. A still higher stringency wash is about 0.1×SSC, 0.1% SDS at65° C. and washing twice for 30 min. The temperature for the washsolutions will ordinarily be at least about 25° C., and for greaterstringency at least about 42° C. Hybridization stringency may beincreased further by using the same conditions as in the hybridizationsteps, with the wash temperature raised about 3° C. to about 5° C., andstringency may be increased even further by using the same conditionsexcept the wash temperature is raised about 6° C. to about 9° C. Foridentification of less closely related homolog, wash steps may beperformed at a lower temperature, e.g., 50° C.

An example of a low stringency wash step employs a solution andconditions of at least 25° C. in 30 mM NaCl, 3 mM trisodium citrate, and0.1% SDS over 30 min. Greater stringency may be obtained at 42° C. in 15mM NaCl, with 1.5 mM trisodium citrate, and 0.1% SDS over 30 min. Evenhigher stringency wash conditions are obtained at 65° C.-68° C. in asolution of 15 mM NaCl, 1.5 mM trisodium citrate, and 0.1% SDS. Washprocedures will generally employ at least two final wash steps.Additional variations on these conditions will be readily apparent tothose skilled in the art (see, for example, U.S. Patent Application No.20010010913).

Stringency conditions can be selected such that an oligonucleotide thatis perfectly complementary to the coding oligonucleotide hybridizes tothe coding oligonucleotide with at least about a 5-10× higher signal tonoise ratio than the ratio for hybridization of the perfectlycomplementary oligonucleotide to a nucleic acid encoding a transcriptionfactor known as of the filing date of the application. It may bedesirable to select conditions for a particular assay such that a highersignal to noise ratio, that is, about 15× or more, is obtained.Accordingly, a subject nucleic acid will hybridize to a unique codingoligonucleotide with at least a 2× or greater signal to noise ratio ascompared to hybridization of the coding oligonucleotide to a nucleicacid encoding known polypeptide. The particular signal will depend onthe label used in the relevant assay, e.g., a fluorescent label, acolorimetric label, a radioactive label, or the like. Labeledhybridization or PCR probes for detecting related polynucleotidesequences may be produced by oligolabeling, nick translation,end-labeling, or PCR amplification using a labeled nucleotide.

Identifying Polynucleotides or Nucleic Acids with Expression Libraries

In addition to hybridization methods, transcription factor homologpolypeptides can be obtained by screening an expression library usingantibodies specific for one or more transcription factors. With theprovision herein of the disclosed transcription factor, andtranscription factor homolog nucleic acid sequences, the encodedpolypeptide(s) can be expressed and purified in a heterologousexpression system (e.g., E. coli) and used to raise antibodies(monoclonal or polyclonal) specific for the polypeptide(s) in question.Antibodies can also be raised against synthetic peptides derived fromtranscription factor, or transcription factor homolog, amino acidsequences. Methods of raising antibodies are well known in the art andare described in Harlow and Lane (1988), Antibodies: A LaboratoryManual, Cold Spring Harbor Laboratory, New York. Such antibodies canthen be used to screen an expression library produced from the plantfrom which it is desired to clone additional transcription factorhomologs, using the methods described above. The selected cDNAs can beconfirmed by sequencing and enzymatic activity.

Sequence Variations

It will readily be appreciated by those of skill in the art, that any ofa variety of polynucleotide sequences are capable of encoding thetranscription factors and transcription factor homolog polypeptides ofthe invention. Due to the degeneracy of the genetic code, many differentpolynucleotides can encode identical and/or substantially similarpolypeptides in addition to those sequences illustrated in the SequenceListing (except CBF polypeptide sequences SEQ ID NOs: 1956, 1958, 1960,or 2204). Nucleic acids having a sequence that differs from thesequences shown in the Sequence Listing, or complementary sequences,that encode functionally equivalent peptides (i.e., peptides having somedegree of equivalent or similar biological activity) but differ insequence from the sequence shown in the Sequence Listing due todegeneracy in the genetic code, are also within the scope of theinvention.

Altered polynucleotide sequences encoding polypeptides include thosesequences with deletions, insertions, or substitutions of differentnucleotides, resulting in a polynucleotide encoding a polypeptide withat least one functional characteristic of the instant polypeptides.Included within this definition are polymorphisms which may or may notbe readily detectable using a particular oligonucleotide probe of thepolynucleotide encoding the instant polypeptides, and improper orunexpected hybridization to allelic variants, with a locus other thanthe normal chromosomal locus for the polynucleotide sequence encodingthe instant polypeptides.

Allelic variant refers to any of two or more alternative forms of a geneoccupying the same chromosomal locus. Allelic variation arises naturallythrough mutation, and may result in phenotypic polymorphism withinpopulations. Gene mutations can be silent (i.e., no change in theencoded polypeptide) or may encode polypeptides having altered aminoacid sequence. The term allelic variant is also used herein to denote aprotein encoded by an allelic variant of a gene. Splice variant refersto alternative forms of RNA transcribed from a gene. Splice variationarises naturally through use of alternative splicing sites within atranscribed RNA molecule, or less commonly between separatelytranscribed RNA molecules, and may result in several mRNAs transcribedfrom the same gene. Splice variants may encode polypeptides havingaltered amino acid sequence. The term splice variant is also used hereinto denote a protein encoded by a splice variant of an mRNA transcribedfrom a gene.

Those skilled in the art would recognize that, for example, G28, SEQ IDNO: 10, represents a single transcription factor; allelic variation andalternative splicing may be expected to occur. Allelic variants of SEQID NO: 9 can be cloned by probing cDNA or genomic libraries fromdifferent individual organisms according to standard procedures. Allelicvariants of the DNA sequence shown in SEQ ID NO: 9, including thosecontaining silent mutations and those in which mutations result in aminoacid sequence changes, are within the scope of the present invention, asare proteins which are allelic variants of SEQ ID NO: 10. cDNAsgenerated from alternatively spliced mRNAs, which retain the propertiesof the transcription factor are included within the scope of the presentinvention, as are polypeptides encoded by such cDNAs and mRNAs. Allelicvariants and splice variants of these sequences can be cloned by probingcDNA or genomic libraries from different individual organisms or tissuesaccording to standard procedures known in the art (see U.S. Pat. No.6,388,064).

Thus, in addition to the sequences set forth in the Sequence Listing(except CBF sequences), the invention also encompasses related nucleicacid molecules that include allelic or splice variants of SEQ ID NO:2N−1, wherein N=1-229, SEQ ID NO: 459-466; 468-487; 491-500; 504;506-511; 516-520; 523-524; 527; 529; 531-533; 538-539; 541-557; 560-568;570-586; 595-596; 598-606; 610-620; 627-634; 640-664; 670-707; 714-719;722-735; 740-741; 743-779; 808-823; 825-834; 838-850; 855-864; 868-889;892-902; 908-909; 914-921; 924-925; 927-932; 935-942; 944-952; 961-965;968-986; 989-993; 995-1010; 1012-1034; 1043-1063; 1074-1080; 1091-1104;1111-1121; 1123-1128; 1134-1138; 1142-1156; 1159-1175; 1187-1190;1192-1199; 1202-1220; 1249-1253; 1258-1262; 1264-1269; 1271-1287;1292-1301; 1303-1309; 1315-1323; 1328-1337; 1340-1341; 1344-1361;1365-1377; 1379-1390; 1393-1394; 1396-1398; 1419-1432; 1434-1452;1455-1456; 1460-1465; 1468-1491; 1499; 1502; 1505-1521; 1523-1527;1529-1532; 1536-1539; 1542-1562; 1567-1571; 1573-1582; 1587-1592;1595-1620; 1625-1644; 1647-1654; 1659-1669; 1671-1673; 1675-1680;1682-1686; 1688-1700; 1706-1709; 1714-1726; 1728-1734; 1738-1742;1744-1753; 1757-1760; 1763-1764; 1766-1768; 1770-1780; 1782-1784;1786-1789; 1791-1804; 1806-1812; 1814-1837; 1847-1856; 1858-1862;1864-1873; 1876-1882; 1885-1896; 1902-1910; 1913-1916; 1921-1928;1931-1936; 1940-1941; 1944-1946, or SEQ ID NO: 2N−1, wherein N=974-1101,and include sequences which are complementary to any of the abovenucleotide sequences. Related nucleic acid molecules also includenucleotide sequences encoding a polypeptide comprising or consistingessentially of a substitution, modification, addition and/or deletion ofone or more amino acid residues compared to the polypeptide as set forthin any of SEQ ID NO: 2N, wherein N=1-229, SEQ ID NO: 467; 488-490;501-503; 505; 512-515; 521-522; 525-526; 528; 530; 534-537; 540;558-559; 569; 587-594; 597; 607-609; 621-626; 635-639; 665-669; 708-713;720-721; 736-739; 742; 780-807; 824; 835-837; 851-854; 865-867; 890-891;903-907; 910-913; 922-923; 926; 933-934; 943; 953-960; 966-967; 987-988;994; 1011; 1035-1042; 1064-1073; 1081-1090; 1105-1110; 1122; 1129-1133;1139-1141; 1157-1158; 1176-1186; 1191; 1200-1201; 1221-1248; 1254-1257;1263; 1270; 1288-1291; 1302; 1310-1314; 1324-1327; 1338-1339; 1342-1343;1362-1364; 1378; 1391-1392; 1395; 1399-1418; 1433; 1453-1454; 1457-1459;1466-1467; 1492-1498; 1500-1501; 1503-1504; 1522; 1528; 1533-1535;1540-1541; 1563-1566; 1572; 1583-1586; 1593-1594; 1621-1624; 1645-1646;1655-1658; 1670; 1674; 1681; 1687; 1701-1705; 1710-1713; 1727;1735-1737; 1743; 1754-1756; 1761-1762; 1765; 1769; 1781; 1785; 1790;1805; 1813; 1838-1846; 1857; 1863; 1874-1875; 1883-1884; 1897-1901;1911-1912; 1917-1920; 1929-1930; 1937-1939; 1942-1943; or SEQ ID NO: 2N,wherein N=974-1101. Such related polypeptides may comprise, for example,additions and/or deletions of one or more N-linked or O-linkedglycosylation sites, or an addition and/or a deletion of one or morecysteine residues.

For example, Table 1 illustrates, e.g., that the codons AGC, AGT, TCA,TCC, TCG, and TCT all encode the same amino acid: serine. Accordingly,at each position in the sequence where there is a codon encoding serine,any of the above trinucleotide sequences can be used without alteringthe encoded polypeptide.

TABLE 1 Amino acid Possible Codons Alanine Ala A GCA GCC GCG GCUCysteine Cys C TGC TGT Aspartic acid Asp D GAC GAT Glutamic acid Glu EGAA GAG Phenylalanine Phe F TTC TTT Glycine Gly G GGA GGC GGG GGTHistidine His H CAC CAT Isoleucine Ile I ATA ATC ATT Lysine Lys KAAA AAG Leucine Leu L TTA TTG CTA CTC CTG CTT Methionine Met M ATGAsparagine Asn N AAC AAT Proline Pro P CCA CCC CCG CCT Glutamine Gln QCAA CAG Arginine Arg R AGA AGG CGA CGC CGG CGT Serine Ser SAGC AGT TCA TCC TCG TCT Threonine Thr T ACA ACC ACG ACT Valine Val VGTA GTC GTG GTT Tryptophan Trp W TGG Tyrosine Tyr Y TAC TAT

Sequence alterations that do not change the amino acid sequence encodedby the polynucleotide are termed “silent” variations. With the exceptionof the codons ATG and TGG, encoding methionine and tryptophan,respectively, any of the possible codons for the same amino acid can besubstituted by a variety of techniques, e.g., site-directed mutagenesis,available in the art. Accordingly, any and all such variations of asequence selected from the above table are a feature of the invention.

In addition to silent variations, other conservative variations thatalter one, or a few amino acids in the encoded polypeptide, can be madewithout altering the function of the polypeptide, these conservativevariants are, likewise, a feature of the invention.

For example, substitutions, deletions and insertions introduced into thesequences provided in the Sequence Listing (except CBF polypeptidesequences SEQ ID NOs: 1956, 1958, 1960, or 2204, listed therein), arealso envisioned by the invention. Such sequence modifications can beengineered into a sequence by site-directed mutagenesis (Wu (ed.)Methods Enzymol. (1993) vol. 217, Academic Press) or the other methodsnoted below Amino acid substitutions are typically of single residues;insertions usually will be on the order of about from 1 to 10 amino acidresidues; and deletions will range about from 1 to 30 residues. Inpreferred embodiments, deletions or insertions are made in adjacentpairs, e.g., a deletion of two residues or insertion of two residues.Substitutions, deletions, insertions or any combination thereof can becombined to arrive at a sequence. The mutations that are made in thepolynucleotide encoding the transcription factor should not place thesequence out of reading frame and should not create complementaryregions that could produce secondary mRNA structure. Preferably, thepolypeptide encoded by the DNA performs the desired function.

Conservative substitutions are those in which at least one residue inthe amino acid sequence has been removed and a different residueinserted in its place. Such substitutions generally are made inaccordance with the Table 2 when it is desired to maintain the activityof the protein. Table 2 shows amino acids which can be substituted foran amino acid in a protein and which are typically regarded asconservative substitutions.

TABLE 2 Conservative Residue Substitutions Ala Ser Arg Lys Asn Gln; HisAsp Glu Gln Asn Cys Ser Glu Asp Gly Pro His Asn; Gln Ile Leu, Val LeuIle; Val Lys Arg; Gln Met Leu; Ile Phe Met; Leu; Tyr Ser Thr; Gly ThrSer; Val Trp Tyr Tyr Trp; Phe Val Ile; Leu

Similar substitutions are those in which at least one residue in theamino acid sequence has been removed and a different residue inserted inits place. Such substitutions generally are made in accordance with theTable 3 when it is desired to maintain the activity of the protein.Table 3 shows amino acids which can be substituted for an amino acid ina protein and which are typically regarded as structural and functionalsubstitutions. For example, a residue in column 1 of Table 3 may besubstituted with a residue in column 2; in addition, a residue in column2 of Table 3 may be substituted with the residue of column 1.

TABLE 3 Residue Similar Substitutions Ala Ser; Thr; Gly; Val; Leu; IleArg Lys; His; Gly Asn Gln; His; Gly; Ser; Thr Asp Glu, Ser; Thr Gln Asn;Ala Cys Ser; Gly Glu Asp Gly Pro; Arg His Asn; Gln; Tyr; Phe; Lys; ArgIle Ala; Leu; Val; Gly; Met Leu Ala; Ile; Val; Gly; Met Lys Arg; His;Gln; Gly; Pro Met Leu; Ile; Phe Phe Met; Leu; Tyr; Trp; His; Val; AlaSer Thr; Gly; Asp; Ala; Val; Ile; His Thr Ser; Val; Ala; Gly Trp Tyr;Phe; His Tyr Trp; Phe; His Val Ala; Ile; Leu; Gly; Thr; Ser; Glu

Substitutions that are less conservative than those in Table 2 can beselected by picking residues that differ more significantly in theireffect on maintaining (a) the structure of the polypeptide backbone inthe area of the substitution, for example, as a sheet or helicalconformation, (b) the charge or hydrophobicity of the molecule at thetarget site, or (c) the bulk of the side chain. The substitutions whichin general are expected to produce the greatest changes in proteinproperties will be those in which (a) a hydrophilic residue, e.g., serylor threonyl, is substituted for (or by) a hydrophobic residue, e.g.,leucyl, isoleucyl, phenylalanyl, valyl or alanyl; (b) a cysteine orproline is substituted for (or by) any other residue; (c) a residuehaving an electropositive side chain, e.g., lysyl, arginyl, or histidyl,is substituted for (or by) an electronegative residue, e.g., glutamyl oraspartyl; or (d) a residue having a bulky side chain, e.g.,phenylalanine, is substituted for (or by) one not having a side chain,e.g., glycine.

Further Modifying Sequences of the Invention—Mutation/Forced Evolution

In addition to generating silent or conservative substitutions as noted,above, the present invention optionally includes methods of modifyingthe sequences of the Sequence Listing. In the methods, nucleic acid orprotein modification methods are used to alter the given sequences toproduce new sequences and/or to chemically or enzymatically modify givensequences to change the properties of the nucleic acids or proteins.

Thus, in one embodiment, given nucleic acid sequences are modified,e.g., according to standard mutagenesis or artificial evolution methodsto produce modified sequences. The modified sequences may be createdusing purified natural polynucleotides isolated from any organism or maybe synthesized from purified compositions and chemicals using chemicalmeans well know to those of skill in the art. For example, Ausubel,supra, provides additional details on mutagenesis methods. Artificialforced evolution methods are described, for example, by Stemmer (1994)Nature 370: 389-391, Stemmer (1994) Proc. Natl. Acad. Sci. 91:10747-10751, and U.S. Pat. Nos. 5,811,238, 5,837,500, and 6,242,568.Methods for engineering synthetic transcription factors and otherpolypeptides are described, for example, by Zhang et al. (2000) J. Biol.Chem. 275: 33850-33860, Liu et al. (2001) J. Biol. Chem. 276:11323-11334, and Isalan et al. (2001) Nature Biotechnol. 19: 656-660.Many other mutation and evolution methods are also available andexpected to be within the skill of the practitioner.

Similarly, chemical or enzymatic alteration of expressed nucleic acidsand polypeptides can be performed by standard methods. For example,sequence can be modified by addition of lipids, sugars, peptides,organic or inorganic compounds, by the inclusion of modified nucleotidesor amino acids, or the like. For example, protein modificationtechniques are illustrated in Ausubel, supra. Further details onchemical and enzymatic modifications can be found herein. Thesemodification methods can be used to modify any given sequence, or tomodify any sequence produced by the various mutation and artificialevolution modification methods noted herein.

Accordingly, the invention provides for modification of any givennucleic acid by mutation, evolution, chemical or enzymatic modification,or other available methods, as well as for the products produced bypracticing such methods, e.g., using the sequences herein as a startingsubstrate for the various modification approaches.

For example, optimized coding sequence containing codons preferred by aparticular prokaryotic or eukaryotic host can be used e.g., to increasethe rate of translation or to produce recombinant RNA transcripts havingdesirable properties, such as a longer half-life, as compared withtranscripts produced using a non-optimized sequence. Translation stopcodons can also be modified to reflect host preference. For example,preferred stop codons for Saccharomyces cerevisiae and mammals are TAAand TGA, respectively. The preferred stop codon for monocotyledonousplants is TGA, whereas insects and E. coli prefer to use TAA as the stopcodon.

The polynucleotide sequences of the present invention can also beengineered in order to alter a coding sequence for a variety of reasons,including but not limited to, alterations which modify the sequence tofacilitate cloning, processing and/or expression of the gene product.For example, alterations are optionally introduced using techniqueswhich are well known in the art, e.g., site-directed mutagenesis, toinsert new restriction sites, to alter glycosylation patterns, to changecodon preference, to introduce splice sites, etc.

Furthermore, a fragment or domain derived from any of the polypeptidesof the invention can be combined with domains derived from othertranscription factors or synthetic domains to modify the biologicalactivity of a transcription factor. For instance, a DNA-binding domainderived from a transcription factor of the invention can be combinedwith the activation domain of another transcription factor or with asynthetic activation domain. A transcription activation domain assistsin initiating transcription from a DNA-binding site. Examples includethe transcription activation region of VP16 or GAL4 (Moore et al. (1998)Proc. Natl. Acad. Sci. 95: 376-381; Aoyama et al. (1995) Plant Cell 7:1773-1785), peptides derived from bacterial sequences (Ma and Ptashne(1987) Cell 51: 113-119) and synthetic peptides (Giniger and Ptashne(1987) Nature 330: 670-672).

Expression and Modification of Polypeptides

Typically, polynucleotide sequences of the invention are incorporatedinto recombinant DNA (or RNA) molecules that direct expression ofpolypeptides of the invention in appropriate host cells, transgenicplants, in vitro translation systems, or the like. Due to the inherentdegeneracy of the genetic code, nucleic acid sequences which encodesubstantially the same or a functionally equivalent amino acid sequencecan be substituted for any listed sequence to provide for cloning andexpressing the relevant homolog.

The transgenic plants of the present invention comprising recombinantpolynucleotide sequences are generally derived from parental plants,which may themselves be non-transformed (or non-transgenic) plants.These transgenic plants may either have a transcription factor gene“knocked out” (for example, with a genomic insertion by homologousrecombination, an antisense or ribozyme construct) or expressed to anormal or wild-type extent. However, overexpressing transgenic “progeny”plants will exhibit greater mRNA levels, wherein the mRNA encodes atranscription factor, that is, a DNA-binding protein that is capable ofbinding to a DNA regulatory sequence and inducing transcription, andpreferably, expression of a plant trait gene. Preferably, the mRNAexpression level will be at least three-fold greater than that of theparental plant, or more preferably at least ten-fold greater mRNA levelscompared to said parental plant, and most preferably at least fifty-foldgreater compared to said parental plant.

Vectors, Promoters, and Expression Systems

The present invention includes recombinant constructs comprising one ormore of the nucleic acid sequences herein. The constructs typicallycomprise a vector, such as a plasmid, a cosmid, a phage, a virus (e.g.,a plant virus), a bacterial artificial chromosome (BAC), a yeastartificial chromosome (YAC), or the like, into which a nucleic acidsequence of the invention has been inserted, in a forward or reverseorientation. In a preferred aspect of this embodiment, the constructfurther comprises regulatory sequences, including, for example, apromoter, operably linked to the sequence. Large numbers of suitablevectors and promoters are known to those of skill in the art, and arecommercially available.

General texts that describe molecular biological techniques usefulherein, including the use and production of vectors, promoters and manyother relevant topics, include Berger, Sambrook, supra and Ausubel,supra. Any of the identified sequences can be incorporated into acassette or vector, e.g., for expression in plants. A number ofexpression vectors suitable for stable transformation of plant cells orfor the establishment of transgenic plants have been described includingthose described in Weissbach and Weissbach (1989) Methods for PlantMolecular Biology, Academic Press, and Gelvin et al. (1990) PlantMolecular Biology Manual, Kluwer Academic Publishers. Specific examplesinclude those derived from a Ti plasmid of Agrobacterium tumefaciens, aswell as those disclosed by Herrera-Estrella et al. (1983) Nature 303:209, Bevan (1984) Nucleic Acids Res. 12: 8711-8721, Klee (1985)Bio/Technology 3: 637-642, for dicotyledonous plants.

Alternatively, non-Ti vectors can be used to transfer the DNA intomonocotyledonous plants and cells by using free DNA delivery techniques.Such methods can involve, for example, the use of liposomes,electroporation, microprojectile bombardment, silicon carbide whiskers,and viruses. By using these methods transgenic plants such as wheat,rice (Christou (1991) Bio/Technology 9: 957-962) and corn (Gordon-Kamm(1990) Plant Cell 2: 603-618) can be produced. An immature embryo canalso be a good target tissue for monocots for direct DNA deliverytechniques by using the particle gun (Weeks et al. (1993) Plant Physiol.102: 1077-1084; Vasil (1993) Bio/Technology 10: 667-674; Wan and Lemeaux(1994) Plant Physiol. 104: 37-48, and for Agrobacterium-mediated DNAtransfer (Ishida et al. (1996) Nature Biotechnol. 14: 745-750).

Typically, plant transformation vectors include one or more cloned plantcoding sequence (genomic or cDNA) under the transcriptional control of5′ and 3′ regulatory sequences and a dominant selectable marker. Suchplant transformation vectors typically also contain a promoter (e.g., aregulatory region controlling inducible or constitutive,environmentally- or developmentally-regulated, or cell- ortissue-specific expression), a transcription initiation start site, anRNA processing signal (such as intron splice sites), a transcriptiontermination site, and/or a polyadenylation signal.

A potential utility for the transcription factor polynucleotidesdisclosed herein is the isolation of promoter elements from these genesthat can be used to program expression in plants of any genes. Eachtranscription factor gene disclosed herein is expressed in a uniquefashion, as determined by promoter elements located upstream of thestart of translation, and additionally within an intron of thetranscription factor gene or downstream of the termination codon of thegene. As is well known in the art, for a significant portion of genes,the promoter sequences are located entirely in the region directlyupstream of the start of translation. In such cases, typically thepromoter sequences are located within 2.0 kb of the start oftranslation, or within 1.5 kb of the start of translation, frequentlywithin 1.0 kb of the start of translation, and sometimes within 0.5 kbof the start of translation.

The promoter sequences can be isolated according to methods known to oneskilled in the art.

Examples of constitutive plant promoters which can be useful forexpressing the TF sequence include: the cauliflower mosaic virus (CaMV)35S promoter, which confers constitutive, high-level expression in mostplant tissues (see, e.g., Odell et al. (1985) Nature 313: 810-812); thenopaline synthase promoter (An et al. (1988) Plant Physiol. 88:547-552); and the octopine synthase promoter (Fromm et al. (1989) PlantCell 1: 977-984).

A variety of plant gene promoters that regulate gene expression inresponse to environmental, hormonal, chemical, developmental signals,and in a tissue-active manner can be used for expression of a TFsequence in plants. Choice of a promoter is based largely on thephenotype of interest and is determined by such factors as tissue (e.g.,seed, fruit, root, pollen, vascular tissue, flower, carpel, etc.),inducibility (e.g., in response to wounding, heat, cold, drought, light,pathogens, etc), timing, developmental stage, and the like. Numerousknown promoters have been characterized and can favorably be employed topromote expression of a polynucleotide of the invention in a transgenicplant or cell of interest. For example, tissue specific promotersinclude: seed-specific promoters (such as the napin, phaseolin or DC3promoter described in U.S. Pat. No. 5,773,697), fruit-specific promotersthat are active during fruit ripening (such as the dru 1 promoter (U.S.Pat. No. 5,783,393), or the 2A11 promoter (U.S. Pat. No. 4,943,674) andthe tomato polygalacturonase promoter (Bird et al. (1988) Plant Mol.Biol. 11: 651-662), root-specific promoters, such as those disclosed inU.S. Pat. Nos. 5,618,988, 5,837,848 and 5,905,186, pollen-activepromoters such as PTA29, PTA26 and PTA13 (U.S. Pat. No. 5,792,929),promoters active in vascular tissue (Ringli and Keller (1998) Plant Mol.Biol. 37: 977-988), flower-specific (Kaiser et al. (1995) Plant Mol.Biol. 28: 231-243), pollen (Baerson et al. (1994) Plant Mol. Biol. 26:1947-1959), carpels (Ohl et al. (1990) Plant Cell 2: 837-848), pollenand ovules (Baerson et al. (1993) Plant Mol. Biol. 22: 255-267),auxin-inducible promoters (such as that described in van der Kop et al.(1999) Plant Mol. Biol. 39: 979-990 or Baumann et al. (1999) Plant Cell11: 323-334), cytokinin-inducible promoter (Guevara-Garcia (1998) PlantMol. Biol. 38: 743-753), promoters responsive to gibberellin (Shi et al.(1998) Plant Mol. Biol. 38: 1053-1060, Willmott et al. (1998) 38:817-825) and the like. Additional promoters are those that elicitexpression in response to heat (Ainley et al. (1993) Plant Mol. Biol.22: 13-23), light (e.g., the pea rbcS-3A promoter, Kuhlemeier et al.(1989) Plant Cell 1: 471-478, and the maize rbcS promoter, Schaffner andSheen (1991) Plant Cell 3: 997-1012); wounding (e.g., wunI, Siebertz etal. (1989) Plant Cell 1: 961-968); pathogens (such as the PR-1 promoterdescribed in Buchel et al. (1999) Plant Mol. Biol. 40: 387-396, and thePDF1.2 promoter described in Manners et al. (1998) Plant Mol. Biol. 38:1071-1080), and chemicals such as methyl jasmonate or salicylic acid(Gatz (1997) Annu. Rev. Plant Physiol. Plant Mol. Biol. 48: 89-108). Inaddition, the timing of the expression can be controlled by usingpromoters such as those acting at senescence (Gan and Amasino (1995)Science 270: 1986-1988); or late seed development (Odell et al. (1994)Plant Physiol. 106: 447-458).

Plant expression vectors can also include RNA processing signals thatcan be positioned within, upstream or downstream of the coding sequence.In addition, the expression vectors can include additional regulatorysequences from the 3′-untranslated region of plant genes, e.g., a 3′terminator region to increase mRNA stability of the mRNA, such as thePI-II terminator region of potato or the octopine or nopaline synthase3′ terminator regions.

Additional Expression Elements

Specific initiation signals can aid in efficient translation of codingsequences. These signals can include, e.g., the ATG initiation codon andadjacent sequences. In cases where a coding sequence, its initiationcodon and upstream sequences are inserted into the appropriateexpression vector, no additional translational control signals may beneeded. However, in cases where only coding sequence (e.g., a matureprotein coding sequence), or a portion thereof, is inserted, exogenoustranscriptional control signals including the ATG initiation codon canbe separately provided. The initiation codon is provided in the correctreading frame to facilitate transcription. Exogenous transcriptionalelements and initiation codons can be of various origins, both naturaland synthetic. The efficiency of expression can be enhanced by theinclusion of enhancers appropriate to the cell system in use.

Expression Hosts

The present invention also relates to host cells which are transducedwith vectors of the invention, and the production of polypeptides of theinvention (including fragments thereof) by recombinant techniques. Hostcells are genetically engineered (i.e., nucleic acids are introduced,e.g., transduced, transformed or transfected) with the vectors of thisinvention, which may be, for example, a cloning vector or an expressionvector comprising the relevant nucleic acids herein. The vector isoptionally a plasmid, a viral particle, a phage, a naked nucleic acid,etc. The engineered host cells can be cultured in conventional nutrientmedia modified as appropriate for activating promoters, selectingtransformants, or amplifying the relevant gene. The culture conditions,such as temperature, pH and the like, are those previously used with thehost cell selected for expression, and will be apparent to those skilledin the art and in the references cited herein, including, Sambrook,supra and Ausubel, supra.

The host cell can be a eukaryotic cell, such as a yeast cell, or a plantcell, or the host cell can be a prokaryotic cell, such as a bacterialcell. Plant protoplasts are also suitable for some applications. Forexample, the DNA fragments are introduced into plant tissues, culturedplant cells or plant protoplasts by standard methods includingelectroporation (Fromm et al. (1985) Proc. Natl. Acad. Sci. 82:5824-5828, infection by viral vectors such as cauliflower mosaic virus(CaMV) (Hohn et al. (1982) Molecular Biology of Plant Tumors AcademicPress, New York, N.Y., pp. 549-560; U.S. Pat. No. 4,407,956), highvelocity ballistic penetration by small particles with the nucleic acideither within the matrix of small beads or particles, or on the surface(Klein et al. (1987) Nature 327: 70-73), use of pollen as vector (WO85/01856), or use of Agrobacterium tumefaciens or A. rhizogenes carryinga T-DNA plasmid in which DNA fragments are cloned. The T-DNA plasmid istransmitted to plant cells upon infection by Agrobacterium tumefaciens,and a portion is stably integrated into the plant genome (Horsch et al.(1984) Science 233: 496-498; Fraley et al. (1983) Proc. Natl. Acad. Sci.80: 4803-4807).

The cell can include a nucleic acid of the invention that encodes apolypeptide, wherein the cell expresses a polypeptide of the invention.The cell can also include vector sequences, or the like. Furthermore,cells and transgenic plants that include any polypeptide or nucleic acidabove or throughout this specification, e.g., produced by transductionof a vector of the invention, are an additional feature of theinvention.

For long-term, high-yield production of recombinant proteins, stableexpression can be used. Host cells transformed with a nucleotidesequence encoding a polypeptide of the invention are optionally culturedunder conditions suitable for the expression and recovery of the encodedprotein from cell culture. The protein or fragment thereof produced by arecombinant cell may be secreted, membrane-bound, or containedintracellularly, depending on the sequence and/or the vector used. Aswill be understood by those of skill in the art, expression vectorscontaining polynucleotides encoding mature proteins of the invention canbe designed with signal sequences which direct secretion of the maturepolypeptides through a prokaryotic or eukaryotic cell membrane.

Modified Amino Acid Residues

Polypeptides of the invention may contain one or more modified aminoacid residues. The presence of modified amino acids may be advantageousin, for example, increasing polypeptide half-life, reducing polypeptideantigenicity or toxicity, increasing polypeptide storage stability, orthe like. Amino acid residue(s) are modified, for example,co-translationally or post-translationally during recombinant productionor modified by synthetic or chemical means.

Non-limiting examples of a modified amino acid residue includeincorporation or other use of acetylated amino acids, glycosylated aminoacids, sulfated amino acids, prenylated (e.g., farnesylated,geranylgeranylated) amino acids, PEG modified (e.g., “PEGylated”) aminoacids, biotinylated amino acids, carboxylated amino acids,phosphorylated amino acids, etc. References adequate to guide one ofskill in the modification of amino acid residues are replete throughoutthe literature.

The modified amino acid residues may prevent or increase affinity of thepolypeptide for another molecule, including, but not limited to,polynucleotide, proteins, carbohydrates, lipids and lipid derivatives,and other organic or synthetic compounds.

Identification of Additional Factors

A transcription factor provided by the present invention can also beused to identify additional endogenous or exogenous molecules that canaffect a phenotype or trait of interest. On the one hand, such moleculesinclude organic (small or large molecules) and/or inorganic compoundsthat affect expression of (i.e., regulate) a particular transcriptionfactor. Alternatively, such molecules include endogenous molecules thatare acted upon either at a transcriptional level by a transcriptionfactor of the invention to modify a phenotype as desired. For example,the transcription factors can be employed to identify one or moredownstream genes that are subject to a regulatory effect of thetranscription factor. In one approach, a transcription factor ortranscription factor homolog of the invention is expressed in a hostcell, e.g., a transgenic plant cell, tissue or explant, and expressionproducts, either RNA or protein, of likely or random targets aremonitored, e.g., by hybridization to a microarray of nucleic acid probescorresponding to genes expressed in a tissue or cell type of interest,by two-dimensional gel electrophoresis of protein products, or by anyother method known in the art for assessing expression of gene productsat the level of RNA or protein. Alternatively, a transcription factor ofthe invention can be used to identify promoter sequences (such asbinding sites on DNA sequences) involved in the regulation of adownstream target. After identifying a promoter sequence, interactionsbetween the transcription factor and the promoter sequence can bemodified by changing specific nucleotides in the promoter sequence orspecific amino acids in the transcription factor that interact with thepromoter sequence to alter a plant trait. Typically, transcriptionfactor DNA-binding sites are identified by gel shift assays. Afteridentifying the promoter regions, the promoter region sequences can beemployed in double-stranded DNA arrays to identify molecules that affectthe interactions of the transcription factors with their promoters(Bulyk et al. (1999) Nature Biotechnol. 17: 573-577).

The identified transcription factors are also useful to identifyproteins that modify the activity of the transcription factor. Suchmodification can occur by covalent modification, such as byphosphorylation, or by protein-protein (homo or -heteropolymer)interactions. Any method suitable for detecting protein-proteininteractions can be employed. Among the methods that can be employed areco-immunoprecipitation, cross-linking and co-purification throughgradients or chromatographic columns, and the two-hybrid yeast system.

The two-hybrid system detects protein interactions in vivo and isdescribed in Chien et al. (1991) Proc. Natl. Acad. Sci. 88: 9578-9582,and is commercially available from Clontech (Palo Alto, Calif.). In sucha system, plasmids are constructed that encode two hybrid proteins: oneconsists of the DNA-binding domain of a transcription activator proteinfused to the TF polypeptide and the other consists of the transcriptionactivator protein's activation domain fused to an unknown protein thatis encoded by a cDNA that has been recombined into the plasmid as partof a cDNA library. The DNA-binding domain fusion plasmid and the cDNAlibrary are transformed into a strain of the yeast Saccharomycescerevisiae that contains a reporter gene (e.g., lacZ) whose regulatoryregion contains the transcription activator's binding site. Eitherhybrid protein alone cannot activate transcription of the reporter gene.Interaction of the two hybrid proteins reconstitutes the functionalactivator protein and results in expression of the reporter gene, whichis detected by an assay for the reporter gene product. Then, the libraryplasmids responsible for reporter gene expression are isolated andsequenced to identify the proteins encoded by the library plasmids.After identifying proteins that interact with the transcription factors,assays for compounds that interfere with the TF protein-proteininteractions can be preformed.

Identification of Modulators

In addition to the intracellular molecules described above,extracellular molecules that alter activity or expression of atranscription factor, either directly or indirectly, can be identified.For example, the methods can entail first placing a candidate moleculein contact with a plant or plant cell. The molecule can be introduced bytopical administration, such as spraying or soaking of a plant, orincubating a plant in a solution containing the molecule, and then themolecule's effect on the expression or activity of the TF polypeptide orthe expression of the polynucleotide monitored. Changes in theexpression of the TF polypeptide can be monitored by use of polyclonalor monoclonal antibodies, gel electrophoresis or the like. Changes inthe expression of the corresponding polynucleotide sequence can bedetected by use of microarrays, Northerns, quantitative PCR, or anyother technique for monitoring changes in mRNA expression. Thesetechniques are exemplified in Ausubel et al. (eds.) Current Protocols inMolecular Biology, John Wiley & Sons (1998, and supplements through2001). Changes in the activity of the transcription factor can bemonitored, directly or indirectly, by assaying the function of thetranscription factor, for example, by measuring the expression ofpromoters known to be controlled by the transcription factor (usingpromoter-reporter constructs), measuring the levels of transcripts usingmicroarrays, Northern blots, quantitative PCR, etc. Such changes in theexpression levels can be correlated with modified plant traits and thusidentified molecules can be useful for soaking or spraying on fruit,vegetable and grain crops to modify traits in plants.

Essentially any available composition can be tested for modulatoryactivity of expression or activity of any nucleic acid or polypeptideherein. Thus, available libraries of compounds such as chemicals,polypeptides, nucleic acids and the like can be tested for modulatoryactivity. Often, potential modulator compounds can be dissolved inaqueous or organic (e.g., DMSO-based) solutions for easy delivery to thecell or plant of interest in which the activity of the modulator is tobe tested. Optionally, the assays are designed to screen large modulatorcomposition libraries by automating the assay steps and providingcompounds from any convenient source to assays, which are typically runin parallel (e.g., in microtiter formats on microplates in roboticassays).

In one embodiment, high throughput screening methods involve providing acombinatorial library containing a large number of potential compounds(potential modulator compounds). Such “combinatorial chemical libraries”are then screened in one or more assays, as described herein, toidentify those library members (particular chemical species orsubclasses) that display a desired characteristic activity. Thecompounds thus identified can serve as target compounds.

A combinatorial chemical library can be, e.g., a collection of diversechemical compounds generated by chemical synthesis or biologicalsynthesis. For example, a combinatorial chemical library such as apolypeptide library is formed by combining a set of chemical buildingblocks (e.g., in one example, amino acids) in every possible way for agiven compound length (i.e., the number of amino acids in a polypeptidecompound of a set length). Exemplary libraries include peptidelibraries, nucleic acid libraries, antibody libraries (see, e.g., Vaughnet al. (1996) Nature Biotechnol. 14: 309-314 and PCT/US96/10287),carbohydrate libraries (see, e.g., Liang et al. Science (1996) 274:1520-1522 and U.S. Pat. No. 5,593,853), peptide nucleic acid libraries(see, e.g., U.S. Pat. No. 5,539,083), and small organic moleculelibraries (see, e.g., benzodiazepines, in Baum Chem. & Engineering NewsJan. 18, 1993, page 33; isoprenoids, U.S. Pat. No. 5,569,588;thiazolidinones and metathiazanones, U.S. Pat. No. 5,549,974;pyrrolidines, U.S. Pat. Nos. 5,525,735 and 5,519,134; morpholinocompounds, U.S. Pat. No. 5,506,337) and the like.

Preparation and screening of combinatorial or other libraries is wellknown to those of skill in the art. Such combinatorial chemicallibraries include, but are not limited to, peptide libraries (see, e.g.,U.S. Pat. No. 5,010,175; Furka, (1991) Int. J. Pept. Prot. Res. 37:487-493; and Houghton et al. (1991) Nature 354: 84-88). Otherchemistries for generating chemical diversity libraries can also beused.

In addition, as noted, compound screening equipment for high-throughputscreening is generally available, e.g., using any of a number of wellknown robotic systems that have also been developed for solution phasechemistries useful in assay systems. These systems include automatedworkstations including an automated synthesis apparatus and roboticsystems utilizing robotic arms. Any of the above devices are suitablefor use with the present invention, e.g., for high-throughput screeningof potential modulators. The nature and implementation of modificationsto these devices (if any) so that they can operate as discussed hereinwill be apparent to persons skilled in the relevant art.

Indeed, entire high-throughput screening systems are commerciallyavailable. These systems typically automate entire procedures includingall sample and reagent pipetting, liquid dispensing, timed incubations,and final readings of the microplate in detector(s) appropriate for theassay. These configurable systems provide high throughput and rapidstart up as well as a high degree of flexibility and customization.Similarly, microfluidic implementations of screening are alsocommercially available.

The manufacturers of such systems provide detailed protocols the varioushigh throughput. Thus, for example, Zymark Corp. provides technicalbulletins describing screening systems for detecting the modulation ofgene transcription, ligand binding, and the like. The integrated systemsherein, in addition to providing for sequence alignment and, optionally,synthesis of relevant nucleic acids, can include such screeningapparatus to identify modulators that have an effect on one or morepolynucleotides or polypeptides according to the present invention.

In some assays it is desirable to have positive controls to ensure thatthe components of the assays are working properly. At least two types ofpositive controls are appropriate. That is, known transcriptionalactivators or inhibitors can be incubated with cells or plants, forexample, in one sample of the assay, and the resulting increase/decreasein transcription can be detected by measuring the resulting increase inRNA levels and/or protein expression, for example, according to themethods herein. It will be appreciated that modulators can also becombined with transcriptional activators or inhibitors to findmodulators that inhibit transcriptional activation or transcriptionalrepression. Either expression of the nucleic acids and proteins hereinor any additional nucleic acids or proteins activated by the nucleicacids or proteins herein, or both, can be monitored.

In an embodiment, the invention provides a method for identifyingcompositions that modulate the activity or expression of apolynucleotide or polypeptide of the invention. For example, a testcompound, whether a small or large molecule, is placed in contact with acell, plant (or plant tissue or explant), or composition comprising thepolynucleotide or polypeptide of interest and a resulting effect on thecell, plant, (or tissue or explant) or composition is evaluated bymonitoring, either directly or indirectly, one or more of: expressionlevel of the polynucleotide or polypeptide, activity (or modulation ofthe activity) of the polynucleotide or polypeptide. In some cases, analteration in a plant phenotype can be detected following contact of aplant (or plant cell, or tissue or explant) with the putative modulator,e.g., by modulation of expression or activity of a polynucleotide orpolypeptide of the invention. Modulation of expression or activity of apolynucleotide or polypeptide of the invention may also be caused bymolecular elements in a signal transduction second messenger pathway andsuch modulation can affect similar elements in the same or anothersignal transduction second messenger pathway.

Subsequences

Also contemplated are uses of polynucleotides, also referred to hereinas oligonucleotides, typically having at least 12 bases, preferably atleast 15, more preferably at least 20, 30, or 50 bases, which hybridizeunder at least highly stringent (or ultra-high stringent orultra-ultra-high stringent conditions) conditions to a polynucleotidesequence described above. The polynucleotides may be used as probes,primers, sense and antisense agents, and the like, according to methodsas noted supra.

Subsequences of the polynucleotides of the invention, includingpolynucleotide fragments and oligonucleotides are useful as nucleic acidprobes and primers. An oligonucleotide suitable for use as a probe orprimer is at least about 15 nucleotides in length, more often at leastabout 18 nucleotides, often at least about 21 nucleotides, frequently atleast about 30 nucleotides, or about 40 nucleotides, or more in length.A nucleic acid probe is useful in hybridization protocols, e.g., toidentify additional polypeptide homologs of the invention, includingprotocols for microarray experiments. Primers can be annealed to acomplementary target DNA strand by nucleic acid hybridization to form ahybrid between the primer and the target DNA strand, and then extendedalong the target DNA strand by a DNA polymerase enzyme. Primer pairs canbe used for amplification of a nucleic acid sequence, e.g., by thepolymerase chain reaction (PCR) or other nucleic-acid amplificationmethods. See Sambrook, supra, and Ausubel, supra.

In addition, the invention includes an isolated or recombinantpolypeptide including a subsequence of at least about 15 contiguousamino acids encoded by the recombinant or isolated polynucleotides ofthe invention. For example, such polypeptides, or domains or fragmentsthereof, can be used as immunogens, e.g., to produce antibodies specificfor the polypeptide sequence, or as probes for detecting a sequence ofinterest. A subsequence can range in size from about 15 amino acids inlength up to and including the full length of the polypeptide.

To be encompassed by the present invention, an expressed polypeptidewhich comprises such a polypeptide subsequence performs at least onebiological function of the intact polypeptide in substantially the samemanner, or to a similar extent, as does the intact polypeptide. Forexample, a polypeptide fragment can comprise a recognizable structuralmotif or functional domain such as a DNA binding domain that activatestranscription, e.g., by binding to a specific DNA promoter region anactivation domain, or a domain for protein-protein interactions.

Production of Transgenic Plants

Modification of Traits

The polynucleotides of the invention are favorably employed to producetransgenic plants with various traits, or characteristics, that havebeen modified in a desirable manner, e.g., to improve the seedcharacteristics of a plant. For example, alteration of expression levelsor patterns (e.g., spatial or temporal expression patterns) of one ormore of the transcription factors (or transcription factor homologs) ofthe invention, as compared with the levels of the same protein found ina wild-type plant, can be used to modify a plant's traits. Anillustrative example of trait modification, improved characteristics, byaltering expression levels of a particular transcription factor isdescribed further in the Examples and the Sequence Listing.

Arabidopsis as a Model System

Arabidopsis thaliana is the object of rapidly growing attention as amodel for genetics and metabolism in plants. Arabidopsis has a smallgenome, and well-documented studies are available. It is easy to grow inlarge numbers and mutants defining important genetically controlledmechanisms are either available, or can readily be obtained. Variousmethods to introduce and express isolated homologous genes are available(see Koncz et al. eds., et al. Methods in Arabidopsis Research (1992) etal. World Scientific, New Jersey, N.J., in “Preface”). Because of itssmall size, short life cycle, obligate autogamy and high fertility,Arabidopsis is also a choice organism for the isolation of mutants andstudies in morphogenetic and development pathways, and control of thesepathways by transcription factors (Koncz supra, p. 72). A number ofstudies introducing transcription factors into A. thaliana havedemonstrated the utility of this plant for understanding the mechanismsof gene regulation and trait alteration in plants. (See, for example,Koncz supra, and U.S. Pat. No. 6,417,428).

Arabidopsis Genes in Transgenic Plants.

Expression of genes which encode transcription factors modify expressionof endogenous genes, polynucleotides, and proteins are well known in theart. In addition, transgenic plants comprising isolated polynucleotidesencoding transcription factors may also modify expression of endogenousgenes, polynucleotides, and proteins. Examples include Peng et al.(1997) et al. Genes and Development 11: 3194-3205, and Peng et al.(1999) Nature 400: 256-261. In addition, many others have demonstratedthat an Arabidopsis transcription factor expressed in an exogenous plantspecies elicits the same or very similar phenotypic response. See, forexample, Fu et al. (2001) Plant Cell 13: 1791-1802; Nandi et al. (2000)Curr. Biol. 10: 215-218; Coupland (1995) Nature 377: 482-483; and Weigeland Nilsson (1995) Nature 377: 482-500.

Homologous Genes Introduced into Transgenic Plants.

Homologous genes that may be derived from any plant, or from any sourcewhether natural, synthetic, semi-synthetic or recombinant, and thatshare significant sequence identity or similarity to those provided bythe present invention, may be introduced into plants, for example, cropplants, to confer desirable or improved traits. Consequently, transgenicplants may be produced that comprise a recombinant expression vector orcassette with a promoter operably linked to one or more sequenceshomologous to presently disclosed sequences. The promoter may be, forexample, a plant or viral promoter.

The invention thus provides for methods for preparing transgenic plants,and for modifying plant traits. These methods include introducing into aplant a recombinant expression vector or cassette comprising afunctional promoter operably linked to one or more sequences homologousto presently disclosed sequences. Plants and kits for producing theseplants that result from the application of these methods are alsoencompassed by the present invention.

Transcription Factors of Interest for the Modification of Plant Traits

Currently, the existence of a series of maturity groups for differentlatitudes represents a major barrier to the introduction of new valuabletraits. Any trait (e.g. disease resistance) has to be bred into each ofthe different maturity groups separately, a laborious and costlyexercise. The availability of single strain, which could be grown at anylatitude, would therefore greatly increase the potential for introducingnew traits to crop species such as soybean and cotton.

For many of the specific effects, traits and utilities listed in Table 4and Table 6 that may be conferred to plants, one or more transcriptionfactor genes may be used to increase or decrease, advance or delay, orimprove or prove deleterious to a given trait. Overexpressing orsuppressing one or more genes can impart significant differences inproduction of plant products, such as different fatty acid ratios. Forexample, overexpression of G720 caused a plant to become more freezingtolerant, but knocking out the same transcription factor impartedgreater susceptibility to freezing. Thus, suppressing a gene that causesa plant to be more sensitive to cold may improve a plant's tolerance ofcold. More than one transcription factor gene may be introduced into aplant, either by transforming the plant with one or more vectorscomprising two or more transcription factors, or by selective breedingof plants to yield hybrid crosses that comprise more than one introducedtranscription factor.

A listing of specific effects and utilities that the presently disclosedtranscription factor genes have on plants, as determined by directobservation and assay analysis, is provided in Table 4. Table 4 showsthe polynucleotides identified by SEQ ID NO; Mendel Gene ID No. (GID);and if the polynucleotide was tested in a transgenic assay. The firstcolumn shows the polynucleotide SEQ ID NO; the second column shows theGID; the third column shows whether the gene was overexpressed (OE) orknocked out (KO) in plant studies; the fourth column shows the trait(s)resulting from the knock out or overexpression of the polynucleotide inthe transgenic plant; the fifth column shows the category of the trait;and the sixth column (“Comment”), includes specific observations madewith respect to the polynucleotide of the first column.

TABLE 4 Traits, trait categories, and effects and utilities thattranscription factor genes have on plants. Polynucleotide GID OE/ SEQ IDNO: No. KO Trait(s) Category Observations 1 G8 OE Flowering timeFlowering time Late flowering 3 G19 OE Erysiphe Disease Increasedtolerance to Erysiphe; repressed by methyl jasmonate and induced by1-aminocyclopropane 1- carboxylic acid (ACC) 5 G22 OE Sodium chlorideAbiotic stress Increased tolerance to high salt 7 G24 OE Morphology:other Dev and morph Reduced size and necrotic patches 9 G28 OE BotrytisDisease Increased tolerance to Botrytis Sclerotinia Disease Increasedtolerance to Sclerotinia Erysiphe Disease Increased resistance toErysiphe 11 G47 OE Stem Dev and morph Altered structure of vasculartissues Osmotic Abiotic stress Better root growth under osmotic stressFlowering time Flowering time Late flowering Architecture Dev and morphAltered architecture and inflorescence development Architecture Dev andmorph Reduced apical dominance 13 G156 KO Seed Dev and morph Seed coloralteration 15 G157 OE Flowering time Flowering time Altered floweringtime (modest level of overexpression triggers early flowering, whereas alarger increase delays flowering) 17 G162 OE Seed oil content Seedbiochemistry Increased seed oil content Seed protein content Seedbiochemistry Increased seed protein content 19 G175 OE Osmotic Abioticstress Increased tolerance to osmotic stress 21 G180 OE Seed oil contentSeed biochemistry Decreased seed oil Flowering time Flowering time Earlyflowering 23 G183 OE Flowering time Flowering time Early flowering Lightresponse Dev and morph Constitutive photomorphogenesis 25 G188 KOFusarium Disease Increased susceptibility to Fusarium Osmotic Abioticstress Better germination under osmotic stress 27 G189 OE Size Dev andmorph Increased leaf size 29 G192 OE Flowering time Flowering time Lateflowering Seed oil content Seed biochemistry Decreased seed oil content31 G196 OE Sodium chloride Abiotic stress Increased tolerance to highsalt 33 G211 OE Leaf insoluble sugars Leaf biochemistry Increase in leafxylose Architecture Dev and morph Reduced apical dominance Leaf Dev andmorph Altered leaf shape 35 G214 OE Flowering time Flowering time Lateflowering Leaf fatty acids Leaf biochemistry Increased leaf fatty acidsSeed prenyl lipids Seed biochemistry Increased seed lutein Leaf prenyllipids Leaf biochemistry Increased leaf chlorophyll and carotenoids 37G226 OE Seed protein content Seed biochemistry Increased seed proteinTrichome Dev and morph Glabrous, lack of trichomes Root Dev and morphIncreased root hairs Sodium chloride Abiotic stress Increased toleranceto high salt Nutrient uptake Abiotic stress Increased tolerance tonitrogen- limited medium 39 G241 KO Seed protein content Seedbiochemistry Increased seed protein content Seed oil content Seedbiochemistry Decreased seed oil Sugar sensing Sugar sensing Decreasedgermination and growth on glucose medium 41 G248 OE Botrytis DiseaseIncreased susceptibility to Botrytis 43 G254 OE Sugar sensing Sugarsensing Decreased germination and growth on glucose medium 45 G256 OECold, chilling Abiotic stress Better germination and growth in cold 47G278 OE Sclerotinia Disease Increased susceptibility to Sclerotinia 49G291 OE Seed oil content Seed biochemistry Increased seed oil content 51G303 OE Osmotic Abiotic stress Better germination on high sucrose andhigh NaCl 53 G312 OE Sodium chloride Abiotic stress Better germinationon high NaCl 55 G325 OE Osmotic Abiotic stress Better germination onhigh sucrose and NaCl 57 G343 OE Glyphosate Herbicide sensitivityIncreased resistance to glyphosate Size Dev and morph Small plant 59G353 OE Osmotic Abiotic stress Increased seedling vigor on polyethyleneglycol (PEG) Size Dev and morph Reduced size Leaf Dev and morph Alteredleaf development Flower Dev and morph Short pedicels, downward pointingsiliques 61 G354 OE Size Dev and morph Reduced size Light response Devand morph Constitutive photomorphogenesis Flower Dev and morph Shortpedicels, downward pointing siliques 63 G361 OE Flowering time Floweringtime Late flowering 65 G362 OE Flowering time Flowering time Lateflowering Size Dev and morph Reduced size Trichome Dev and morph Ectopictrichome formation, increased trichome number Morphology: other Dev andmorph Increased pigmentation in seed and embryos, and in other organs 67G371 OE Botrytis Disease Increased susceptibility to Botrytis 69 G390 OEArchitecture Dev and morph Altered shoot development 71 G391 OEArchitecture Dev and morph Altered shoot development 73 G409 OE ErysipheDisease Increased tolerance to Erysiphe 75 G427 OE Seed oil content Seedbiochemistry Increased oil content Seed protein content Seedbiochemistry Decreased protein content 77 G438 KO Stem Dev and morphReduced lignin Architecture Dev and morph Reduced branching 79 G450 OESeed Dev and morph Increased seed size 81 G464 OE Heat Abiotic stressBetter germination and growth in heat 83 G470 OE Fertility Dev and morphShort stamen filaments 85 G477 OE Sclerotinia Disease Increasedsusceptibility to Sclerotinia Oxidative Abiotic stress Increasedsensitivity to oxidative stress 87 G481 OE Sugar sensing Sugar sensingBetter germination on sucrose media Drought Abiotic stress Increasedtolerance to drought 89 G482 OE Sodium chloride Abiotic stress Increasedtolerance to high salt 91 G484 KO Seed glucosinolates Seed biochemistryAltered glucosinolate profile 93 G489 OE Osmotic Abiotic stressIncreased tolerance to osmotic stress 95 G490 OE Flowering timeFlowering time Early flowering 97 G504 OE Seed oil composition Seedbiochemistry Decreased seed oil composition and content; increase in18:2 fatty acid and decrease in 20:1 fatty acid 99 G509 KO Seed oilcontent Seed biochemistry Increased total seed oil and protein Seedprotein content Seed biochemistry content 101 G519 OE Seed oil contentSeed biochemistry Increased seed oil content 103 G545 OE Sodium chlorideAbiotic stress Susceptible to high salt Erysiphe Disease Increasedsusceptibility to Erysiphe Pseudomonas Disease Increased susceptibilityto Pseudomonas Fusarium Disease Increased susceptibility to FusariumNutrient uptake Abiotic stress Increased tolerance to phosphate-freemedium 105 G546 OE Hormone sensitivity Hormone sensitivity Decreasedsensitivity to abscisic acid (ABA) 107 G561 OE Seed oil content Seedbiochemistry Increased seed oil content Nutrient uptake Abiotic stressIncreased tolerance to potassium-free medium 109 G562 OE Flowering timeFlowering time Late flowering 111 G567 OE Seed oil content Seedbiochemistry Increased total seed oil/protein content Seed proteincontent Seed biochemistry Increased total seed oil/protein content Sugarsensing Sugar sensing Decreased seedling vigor on high glucose 113 G568OE Architecture Dev and morph Altered branching 115 G584 OE Seed Dev andmorph Large seeds 117 G585 OE Trichome Dev and morph Reduced trichomedensity 119 G590 KO Seed oil content Seed biochemistry Increased seedoil content OE Flowering time Flowering time Early flowering 121 G594 OESclerotinia Disease Increased susceptibility to Sclerotinia 123 G597 OESeed protein content Seed biochemistry Altered seed protein content 125G598 OE Seed oil content Seed biochemistry Increased seed oil 127 G634OE Trichome Dev and morph Increased trichome density and size 129 G635OE Variegation Dev and morph Altered coloration 131 G636 OE SenescenceDev and morph Premature senescence 133 G638 OE Flower Dev and morphAltered flower development 135 G652 KO Seed prenyl lipids Seedbiochemistry Increase in alpha-tocopherol 137 G663 OE Biochemistry:other Biochem: misc Increased anthocyanins in leaf, root, seed 139 G664OE Cold, chilling Abiotic stress Better germination and growth in cold141 G674 OE Leaf Dev and morph Dark green, upwardly oriented leaves 143G676 OE Trichome Dev and morph Reduced trichome number, ectopic trichomeformation 145 G680 OE Sugar sensing Sugar sensing Reduced germination onglucose medium 147 G682 OE Trichome Dev and morph Glabrous, lack oftrichomes Heat Abiotic stress Better germination and growth in heat RootDev and morph Increased root hairs 149 G715 OE Seed oil content Seedbiochemistry Increased seed oil content 151 G720 OE Freezing Abioticstress More freezing tolerant KO Freezing Abiotic stress Increasedsusceptibility to freezing 153 G736 OE Flowering time Flowering timeLate flowering Leaf Dev and morph Altered leaf shape 155 G748 OE Seedprenyl lipids Seed biochemistry Increased lutein content Stem Dev andmorph More vascular bundles in stem Flowering time Flowering time Lateflowering 157 G779 OE Fertility Dev and morph Reduced fertility FlowerDev and morph Homeotic transformations 159 G789 OE Flowering timeFlowering time Early flowering 161 G801 OE Sodium chloride Abioticstress Better germination on high NaCl 163 G849 KO Seed oil content Seedbiochemistry Increased seed oil content Seed protein content Seedbiochemistry Altered seed protein content 165 G859 OE Flowering timeFlowering time Late flowering 167 G864 OE Heat Abiotic stress Bettergermination in heat 169 G867 OE Sodium chloride Abiotic stress Betterseedling vigor on high salt Sugar sensing Sugar sensing Better seedlingvigor on high sucrose 171 G869 OE Seed oil composition Seed biochemistryAltered seed fatty acids 173 G877 KO Embryo lethal Dev and morph Embryolethal phenotype: potential herbicide target 175 G881 OE ErysipheDisease Increased susceptibility to Erysiphe 177 G892 KO Seed proteincontent Seed biochemistry Altered seed protein content Seed oil contentSeed biochemistry Altered seed oil content 179 G896 KO Fusarium DiseaseIncreased susceptibility to Fusarium 181 G910 OE Flowering timeFlowering time Late flowering 183 G911 OE Nutrient uptake Abiotic stressIncreased growth on potassium-free medium 185 G912 OE Freezing Abioticstress Freezing tolerant Drought Abiotic stress Increased survival indrought Morphology: other Dev and morph conditions Sugar sensing Sugarsensing Dark green color Reduced cotyledon expansion in glucose 187 G913OE Freezing Abiotic stress Increased tolerance to freezing Floweringtime Flowering time Late flowering Drought Abiotic stress Increasedtolerance to drought 189 G922 OE Osmotic Abiotic stress Bettergermination on high sucrose Sodium chloride Abiotic stress Bettergermination, increased root growth on high salt 191 G926 KO Hormonesensitivity Hormone sensitivity Reduced sensitivity to ABA OsmoticAbiotic stress Increased tolerance to osmotic stress (salt and sucrose)193 G961 KO Seed oil content Seed biochemistry Increased seed oilcontent 195 G971 OE Flowering time Flowering time Late flowering 197G974 OE Seed oil content Seed biochemistry Altered seed oil content 199G975 OE Leaf fatty acids Leaf biochemistry Increased wax in leaves 201G979 KO Seed Dev and morph Altered seed development, ripening, andgermination 203 G987 KO Leaf fatty acids Leaf biochemistry Reduction in16:3 fatty acids Leaf prenyl lipids Leaf biochemistry Alteredchlorophyll, tocopherol, carotenoid 205 G988 OE Seed protein contentSeed biochemistry Increased seed protein content Flower Dev and morphEnlarged floral organs, short pedicels Architecture Dev and morphReduced lateral branching Stem Dev and morph Thicker stem, altereddistribution of vascular bundles 207 G1040 OE Seed Dev and morph Smallerand more rounded seeds 209 G1047 OE Fusarium Disease Increased toleranceto Fusarium 211 G1051 OE Flowering time Flowering time Late flowering213 G1052 OE Flowering time Flowering time Late flowering 215 G1062 KOSeed Dev and morph Altered seed shape 217 G1063 OE Leaf Dev and morphAltered leaf shape, dark green color Inflorescence Dev and morph Alteredinflorescence development Flower Dev and morph Altered flowerdevelopment, ectopic carpel tissue 219 G1064 OE Botrytis DiseaseIncreased sensitivity to Botrytis 221 G1069 OE Hormone sensitivityHormone sensitivity Reduced ABA sensitivity Osmotic Abiotic stressBetter germination under osmotic stress 223 G1073 OE Size Dev and morphSubstantially increased plant size Seed Dev and morph Increased seedyield Drought Abiotic stress Increased tolerance to drought 225 G1075 OEFlower Dev and morph Reduced or absent petals, sepals and stamens 227G1084 OE Botrytis Disease Increased susceptibility to Botrytis 229 G1089KO Osmotic Abiotic stress Better germination under osmotic stress 231G1134 OE Hormone sensitivity Hormone sensitivity Altered response toethylene: longer hypocotyls and lack of apical hook 233 G1140 OE FlowerDev and morph Altered flower development 235 G1143 OE Seed oil contentSeed biochemistry Altered seed oil content 237 G1146 OE Leaf Dev andmorph Altered leaf development 239 G1196 KO Botrytis Disease Increasedsusceptibility to Botrytis 241 G1198 OE Seed oil content Seedbiochemistry Increased seed oil content 243 G1225 OE Flowering timeFlowering time Early flowering Sugar sensing Sugar sensing Bettergermination on sucrose and glucose media 245 G1226 OE Seed oil contentSeed biochemistry Increased seed oil content 247 G1229 OE Seed oilcontent Seed biochemistry Decreased seed oil content 249 G1255 OEBotrytis Disease Increased susceptibility to Botrytis Seed Dev and morphIncreased seed size Morphology: other Dev and morph Reduced apicaldominance 251 G1266 OE Erysiphe Disease Increased tolerance to Erysiphe253 G1275 OE Architecture Dev and morph Reduced apical dominance 255G1305 OE Heat Abiotic stress Reduced chlorosis in heat 257 G1322 OEChilling Abiotic stress Increased seedling vigor in cold Size Dev andmorph Reduced size Leaf glucosinolates Leaf biochemistry Increase inM39480 Light response Dev and morph Photomorphogenesis in the dark 259G1323 OE Seed oil content Seed biochemistry Decreased seed oil Seedprotein content Seed biochemistry Increased seed protein 261 G1330 OEHormone sensitivity Hormone sensitivity Ethylene insensitive whengerminated in the dark on ACC 263 G1331 OE Light response Dev and morphConstitutive photomorphogenesis 265 G1332 OE Trichome Dev and morphReduced trichome density 267 G1363 OE Fusarium Disease Increasedtolerance to Fusarium 269 G1411 OE Architecture Dev and morph Loss ofapical dominance 271 G1417 KO Seed oil composition Seed biochemistryIncrease in 18:2, decrease in 18:3 fatty acids 273 G1419 OE Seed proteincontent Seed biochemistry Increased seed protein 275 G1449 OE Flower Devand morph Altered flower structure 277 G1451 OE Morphology: other Devand morph Increased plant size OE Leaf Dev and morph Large leaf size KOSeed oil content Seed biochemistry Altered seed oil content 279 G1452 OETrichome Dev and morph Reduced trichome density Leaf Dev and morphAltered leaf shape, dark green color Hormone sensitivity Hormonesensitivity Reduced sensitivity to ABA Osmotic Abiotic stress Bettergermination on sucrose and salt Flowering time Flowering time Lateflowering 281 G1463 OE Senescence Dev and morph Premature senescence 283G1471 OE Seed oil content Seed biochemistry Increased seed oil content285 G1478 OE Seed protein content Seed biochemistry Decreased seedprotein content Flowering time Flowering time Late flowering Seed oilcontent Seed biochemistry Increased seed oil content 287 G1482 KOBiochemistry: other Biochem: misc Increased anthocyanins OE Root Dev andmorph Increased root growth 289 G1488 OE Seed protein content Seedbiochemistry Altered seed protein content Light response Dev and morphConstitutive photomorphogenesis Architecture Dev and morph Reducedapical dominance, shorter stems 291 G1494 OE Flowering time Floweringtime Early flowering Light response Dev and morph Long hypocotyls,altered leaf shape Leaf Dev and morph Pale green leaves, altered leafshape 293 G1496 OE Seed oil content Seed biochemistry Altered seed oilcontent 295 G1499 OE Morphology: other Dev and morph Dark green colorArchitecture Dev and morph Altered plant architecture Flower Dev andmorph Altered floral organ identity and development 297 G1519 KO Embryolethal Dev and morph Embryo lethal phenotype: potential herbicide target299 G1526 KO Seed oil content Seed biochemistry Increased seed oilcontent 301 G1540 OE Morphology: other Dev and morph Reduced celldifferentiation in meristem 303 G1543 OE Architecture Dev and morphAltered architecture, compact plant Morphology: other Dev and morph Darkgreen color Seed oil content Seed biochemistry Decreased seed oil Leafprenyl lipids Leaf biochemistry Increase in chlorophyll a and b 305G1634 OE Seed oil content Seed biochemistry Increased seed oil contentSeed protein content Decreased seed protein content 307 G1637 OE Seedprotein content Seed biochemistry Altered seed protein content 309 G1640OE Seed oil content Seed biochemistry Increased seed oil 311 G1645 OEInflorescence Dev and morph Altered inflorescence structure 313 G1646 OESeed oil content Seed biochemistry Increased seed oil content 315 G1652OE Seed protein content Seed biochemistry Increased seed protein content317 G1672 OE Seed oil content Seed biochemistry Altered seed oil content319 G1677 OE Seed protein content Seed biochemistry Altered seed proteincontent Seed oil content Seed biochemistry Altered seed oil content 321G1749 OE Morphology: other Dev and morph Formation of necrotic lesions323 G1750 OE Seed oil content Seed biochemistry Increased seed oilcontent 325 G1756 OE Botrytis Disease Increased susceptibility toBotrytis 327 G1765 OE Seed oil content Seed biochemistry Increased seedoil content 329 G1777 OE Seed oil content Seed biochemistry Increasedseed oil content Seed protein content Seed biochemistry Decreased seedprotein content 331 G1792 OE Leaf Dev and morph Dark green, shiny leavesErysiphe Disease Increased resistance to Erysiphe Botrytis DiseaseIncreased resistance to Botrytis Fusarium Disease Increased resistanceto Fusarium Nutrient uptake Abiotic stress Increased tolerance tonitrogen- limited medium 333 G1793 OE Seed oil content Seed biochemistryIncreased seed oil content 335 G1794 OE Architecture Dev and morphAltered architecture, bushier plant Architecture Dev and morph Reducedapical dominance Light response Dev and morph Constitutivephotomorphogenesis Osmotic Abiotic stress Increased sensitivity to highPEG Nutrient uptake Abiotic stress Reduced root growth 337 G1804 OEFlowering time Flowering time Late flowering Sugar sensing Sugar sensingAltered sugar sensing: more sensitive to glucose in germination assays339 G1818 OE Seed protein content Seed biochemistry Increased proteincontent 341 G1820 OE Flowering time Flowering time Early floweringHormone sensitivity Hormone sensitivity Reduced ABA sensitivity Seedprotein content Seed biochemistry Increased seed protein content OsmoticAbiotic stress Better germination in high NaCl Drought Abiotic stressIncreased tolerance to drought 343 G1836 OE Sodium chloride Abioticstress Better germination in high salt Drought Abiotic stress Increasedtolerance to drought 345 G1838 OE Seed oil content Seed biochemistryIncreased seed oil content 347 G1841 OE Heat Abiotic stress Bettergermination under heat stress Flowering time Flowering time Earlyflowering 349 G1842 OE Flowering time Flowering time Early flowering 351G1843 OE Flowering time Flowering time Early flowering 353 G1852 OEOsmotic Abiotic stress Better root growth under osmotic stress 355 G1863OE Leaf Dev and morph Altered leaf shape and coloration 357 G1880 KOBotrytis Disease Increased resistance to Botrytis 359 G1895 OE Floweringtime Flowering time Late flowering 361 G1902 OE Seed oil content Seedbiochemistry Increased seed oil content 363 G1903 OE Seed proteincontent Seed biochemistry Decreased seed protein content 365 G1919 OEBotrytis Disease Increased tolerance to Botrytis 367 G1927 OESclerotinia Disease Increased tolerance to Sclerotinia 369 G1930 OEOsmotic Abiotic stress Better germination under osmotic stress 371 G1936KO Sclerotinia Disease Increased susceptibility to Sclerotinia BotrytisDisease Increased susceptibility to Botrytis 373 G1944 OE Senescence Devand morph Early senescence 375 G1946 OE Seed oil content Seedbiochemistry Increased seed oil content Seed protein content Seedbiochemistry Decreased seed protein content Flowering time Floweringtime Early flowering Nutrient uptake Abiotic stress Increased rootgrowth on phosphate- free media 377 G1947 KO Fertility Dev and morphReduced fertility 379 G1948 OE Seed oil content Seed biochemistryIncreased seed oil content 381 G1950 OE Botrytis Disease Increasedtolerance to Botrytis 383 G1958 KO Morphology: other Dev and morphReduced size and root mass Seed oil content Seed biochemistry Increasedseed oil content Seed protein content Seed biochemistry Increased seedprotein content. 385 G2007 OE Flowering time Flowering time Lateflowering 387 G2010 OE Flowering time Flowering time Early flowering 389G2053 OE Osmotic Abiotic stress Increased root growth under osmoticstress 391 G2059 OE Seed oil content Seed biochemistry Altered seed oilcontent Seed protein content Seed biochemistry Altered seed proteincontent 393 G2085 OE Seed Dev and morph Increased seed size and alteredseed color 395 G2105 OE Seed Dev and morph Large, pale seeds 397 G2110OE Sodium chloride Abiotic stress Increased tolerance to high salt 399G2114 OE Seed Dev and morph Increased seed size 401 G2117 OE Seedprotein content Seed biochemistry Increased seed protein content 403G2123 OE Seed oil content Seed biochemistry Increased seed oil content405 G2130 OE Heat Abiotic stress Better germination in heat 407 G2133 OEGlyphosate Herbicide sensitivity Increased tolerance to glyphosateFlowering time Flowering time Late flowering 409 G2138 OE Seed oilcontent Seed biochemistry Increased seed oil content 411 G2140 OEHormone sensitivity Hormone sensitivity Decreased sensitivity to ABAOsmotic Abiotic stress Better germination on high NaCl and sucrose 413G2143 OE Inflorescence Dev and morph Altered inflorescence developmentLeaf Dev and morph Altered leaf shape, dark green color Flower Dev andmorph Altered flower development, ectopic carpel tissue 415 G2144 OEFlowering time Flowering time Early flowering Leaf Dev and morph Palegreen leaves, altered leaf shape Light response Dev and morph Longhypocotyls, altered leaf shape 417 G2153 OE Osmotic Abiotic stressBetter germination under osmotic stress 419 G2155 OE Flowering timeFlowering time Late flowering 421 G2192 OE Seed oil composition Seedbiochemistry Altered seed fatty acid composition 423 G2295 OE Floweringtime Flowering time Early flowering 425 G2340 OE Seed glucosinolatesSeed biochemistry Altered glucosinolate profile 427 G2343 OE Seed oilcontent Seed biochemistry Increased seed oil content 429 G2346 OEMorphology: other Dev and morph Enlarged seedlings 431 G2347 OEFlowering time Flowering time Early flowering 433 G2379 OE OsmoticAbiotic stress Increased seedling vigor on high sucrose media 435 G2430OE Heat Abiotic stress Increased tolerance to heat Size Dev and morphIncreased leaf size, faster development 437 G2505 OE Drought Abioticstress Increased tolerance to drought 439 G2509 OE Seed oil content Seedbiochemistry Decreased seed oil content Seed protein content Seedbiochemistry Increased seed protein content Seed prenyl lipids Seedbiochemistry Increase in alpha-tocopherol Architecture Dev and morphReduced apical dominance Flowering time Flowering time Early flowering441 G2517 OE Glyphosate Herbicide sensitivity Increased tolerance toglyphosate 443 G2520 OE Seed prenyl lipids Seed biochemistry Alteredtocopherol composition 445 G2555 OE Light response Dev and morphConstitutive photomorphogenesis Botrytis Disease Increasedsusceptibility to Botrytis 447 G2557 OE Leaf Dev and morph Altered leafshape, dark green color Flower Dev and morph Altered flower development,ectopic carpel tissue 449 G2583 OE Leaf Dev and morph Glossy, shinyleaves 451 G2701 OE Osmotic Abiotic stress Better germination on highNaCl and sucrose 453 G2719 OE Osmotic Abiotic stress Increased seedlingvigor on high sucrose 455 G2789 OE Osmotic Abiotic stress Bettergermination on high sucrose Hormone sensitivity Hormone sensitivityReduced ABA sensitivity 457 G2830 KO Seed oil content Seed biochemistryIncreased seed oil content 1951 G12 KO Hormone sensitivity Hormonesensitivity Increased sensitivity to ACC OE Morphology: other Dev andmorph Leaf and hypocotyl necrosis 1953 G30 OE Leaf Dev and morph Glossygreen leaves Light response Dev and morph Shade avoidance 1975 G231 OELeaf fatty acids Leaf biochemistry Increased leaf unsaturated fattyacids Seed oil content Seed biochemistry Increased seed oil content Seedprotein content Seed biochemistry Decreased seed protein content 1979G247 OE Trichome Dev and morph Altered trichome distribution, reducedtrichome density 1991 G370 KO Size Dev and morph Reduced size, shinyleaves OE Trichome Dev and morph Ectropic trichome formation 2009 G485OE Flowering time Flowering time Early flowering KO Flowering timeFlowering time Late flowering 2061 G839 OE Nutrient uptake Abioticstress Increased tolerance to nitrogen- limited medium 2099 G1357 OELeaf Dev and morph Altered leaf shape, dark green leaves ChillingAbiotic stress Increased tolerance to cold Hormone sensitivity Hormonesensitivity Insensitive to ABA Flowering time Flowering time Lateflowering 2126 G1646 OE Seed oil content Seed oil content Increased seedoil content 2142 G1816 OE Sugar sensing Sugar sensing Increasedtolerance to glucose Nutrient uptake Abiotic stress Altered C/N sensing;less anthocyanin on nitrogen-limited medium Osmotic Abiotic stressIncreased tolerance to osmotic stress Root Dev and morph Increased roothairs Trichome Dev and morph Glabrous leaves Nutrient uptake Abioticstress Increased tolerance to nitrogen- limited medium 2147 G1888 OESize Dev and morph Reduced size, dark green leaves 2153 G1945 OEFlowering time Flowering time Late flowering Leaf Dev and morph Alteredleaf shape 2195 G2826 OE Flower Dev and morph Aerial rosettes TrichomeDev and morph Ectropic trichome formation 2197 G2838 OE Trichome Dev andmorph Increased trichome density Flowering time Flosering time Lateflowering Flower Dev and morph Flower: multiple alterations Flower Devand morph Aerial rosettes Leaves Dev and morph Dark green leaves SizeDev and morph Increased seedling size 2199 G2839 OE Osmotic stress Devand morph Better germination on high sucrose Inflorescence Dev and morphDownward pedicels Size Abiotic stress Reduced size

Table 5 shows the polypeptides identified by SEQ ID NO; Mendel Gene ID(GID) No.; the transcription factor family to which the polypeptidebelongs, and conserved domains of the polypeptide. The first columnshows the polypeptide SEQ ID NO; the third column shows thetranscription factor family to which the polynucleotide belongs; and thefourth column shows the amino acid residue positions of the conserveddomain in amino acid (AA) co-ordinates.

TABLE 5 Gene families and conserved domains Polypeptide GID ConservedDomains in SEQ ID NO: No. Family Amino Acid Coordinates 2 G8 AP2151-217, 243-296 4 G19 AP2  76-145 6 G22 AP2  89-157 8 G24 AP2 25-93 10G28 AP2 145-213 12 G47 AP2 11-80 14 G156 MADS  2-57 16 G157 MADS  2-5718 G162 MADS  2-57 20 G175 WRKY 178-234, 372-428 22 G180 WRKY 118-174 24G183 WRKY 307-363 26 G188 WRKY 175-222 28 G189 WRKY 240-297 30 G192 WRKY128-185 32 G196 WRKY 223-283 34 G211 MYB-R1 R2R3  24-137 36 G214MYB-related 22-71 38 G226 MYB-related 28-78 40 G241 MYB-R1 R2R3  14-11442 G248 MYB-R1 R2R3 264-332 44 G254 MYB-related  62-106 46 G256 MYB-R1R2R3  13-115 48 G278 AKR  2-593 50 G291 MISC 132-160 52 G303 HLH/MYC 92-161 54 G312 SCR 320-336 56 G325 Z-CO-like 5-28, 48-71 58 G343GATA/Zn 178-214 60 G353 Z-C2H2 41-61, 84-104 62 G354 Z-C2H2 42-62,88-109 64 G361 Z-C2H2 43-63 66 G362 Z-C2H2 62-82 68 G371 RING/C3HC421-74 70 G390 HB 18-81 72 G391 HB 25-85 74 G409 HB  64-124 76 G427 HB307-370 78 G438 HB 22-85 80 G450 IAA 6-14, 78-89, 112-128, 180-213 82G464 IAA 20-28, 71-82, 126-142, 187-224 84 G470 ARF  61-393 86 G477 SBP108-233 88 G481 CAAT  20-109 90 G482 CAAT  25-116 92 G484 CAAT  11-10494 G489 CAAT  57-156 96 G490 CAAT  48-143 98 G504 NAC  19-174 100 G509NAC  13-169 102 G519 NAC  11-104 104 G545 Z-C2H2 82-102, 136-154 106G546 RING/C3H2C3 114-155 108 G561 bZIP 248-308 110 G562 bZIP 253-315 112G567 bZIP 210-270 114 G568 bZIP 215-265 116 G584 HLH/MYC 401-494 118G585 HLH/MYC 436-501 120 G590 HLH/MYC 202-254 122 G594 HLH/MYC 140-204124 G597 AT-hook 97-104, 137-144 126 G598 DBP 205-263 128 G634 TH62-147, 189-245 130 G635 TH 239-323 132 G636 TH 55-145, 405-498 134 G638TH 119-206 136 G652 Z-CLDSH 28-49, 137-151, 182-196 138 G663 MYB-R1 R2R3 9-111 140 G664 MYB-R1 R2R3  13-116 142 G674 MYB-R1 R2R3  20-120 144G676 MYB-R1 R2R3  17-119 146 G680 MYB-related 24-70 148 G682 MYB-related27-63 150 G715 CAAT  60-132 152 G720 GARP 301-349 154 G736 Z-Dof  54-111156 G748 Z-Dof 112-140 158 G779 HLH/MYC 126-182 160 G789 HLH/MYC 253-313162 G801 PCF 32-93 164 G849 BPF-1 324-413, 504-583 166 G859 MADS  3-56168 G864 AP2 119-186 170 G867 AP2  59-124 172 G869 AP2 109-177 174 G877WRKY 272-328, 487-603 176 G881 WRKY 176-233 178 G892 RING/C3H2C3 177-270180 G896 Z-LSDlike 18-39 182 G910 Z-CO-like 14-37, 77-103 184 G911RING/C3H2C3  86-129 186 G912 AP2  51-118 188 G913 AP2  62-128 190 G922SCR 225-242 192 G926 CAAT 131-225 194 G961 NAC  15-140 196 G971 AP2120-186 198 G974 AP2  81-140 200 G975 AP2  4-71 202 G979 AP2 63-139,165-233 204 G987 SCR 428-432, 704-708 206 G988 SCR 178-195 208 G1040GARP 109-158 210 G1047 bZIP 129-180 212 G1051 bZIP 189-250 214 G1052bZIP 201-261 216 G1062 HLH/MYC 308-359 218 G1063 HLH/MYC 131-182 220G1064 PCF 116-179 222 G1069 AT-hook 67-74 224 G1073 AT-hook 33-42,78-175 226 G1075 AT-hook 78-85 228 G1084 BZIPT2 1-53, 490-619 230 G1089BZIPT2 425-500 232 G1134 HLH/MYC 198-247 234 G1140 MADS  2-57 236 G1143HLH/MYC 33-82 238 G1146 PAZ 886-896 240 G1196 AKR 179-254 242 G1198 bZIP173-223 244 G1225 HLH/MYC  78-147 246 G1226 HLH/MYC 115-174 248 G1229HLH/MYC 102-160 250 G1255 Z-CO-like 18-56 252 G1266 AP2  79-147 254G1275 WRKY 113-169 256 G1305 MYB-R1 R2R3  15-118 258 G1322 MYB-R1 R2R3 26-130 260 G1323 MYB-R1 R2R3  15-116 262 G1330 MYB-R1 R2R3  28-134 264G1331 MYB-R1 R2R3  8-109 266 G1332 MYB-R1 R2R3  13-116 268 G1363 CAAT174-226 270 G1411 AP2  87-154 272 G1417 WRKY 239-296 274 G1419 AP2 69-137 276 G1449 IAA 48-53, 74-107, 122-152 278 G1451 ARF  22-357 280G1452 NAC  30-177 282 G1463 NAC  9-156 284 G1471 Z-C2H2 49-70 286 G1478Z-CO-like 32-76 288 G1482 Z-CO-like  5-63 290 G1488 GATA/Zn 221-246 292G1494 HLH/MYC 261-311 294 G1496 HLH/MYC 184-248 296 G1499 HLH/MYC118-181 298 G1519 RING/C3HC4 327-364 300 G1526 SWI/SNF 493-620, 864-1006302 G1540 HB 35-98 304 G1543 HB 135-195 306 G1634 MYB-related 129-180308 G1637 MYB-related 109-173 310 G1640 MYB-R1 R2R3  14-115 312 G1645MYB-R1 R2R3  90-210 314 G1646 CAAT  72-162 316 G1652 HLH/MYC 143-215 318G1672 NAC  41-194 320 G1677 NAC  17-181 322 G1749 AP2  84-155 324 G1750AP2 107-173 326 G1756 WRKY 141-197 328 G1765 NAC  20-140 330 G1777RING/C3HC4 124-247 332 G1792 AP2 17-85 334 G1793 AP2 179-255, 281-349336 G1794 AP2 182-249 338 G1804 bZIP 357-407 340 G1818 CAAT  36-113 342G1820 CAAT  70-133 344 G1836 CAAT  30-164 346 G1838 AP2 229-305, 330-400348 G1841 AP2  83-150 350 G1842 MADS  2-57 352 G1843 MADS  2-57 354G1852 AKR  1-600 356 G1863 GRF-like  77-186 358 G1880 Z-C2H2 69-89,111-139 360 G1895 Z-Dof  55-110 362 G1902 Z-Dof 31-59 364 G1903 Z-Dof134-180 366 G1919 RING/C3HC4 214-287 368 G1927 NAC  17-188 370 G1930 AP2 59-124 372 G1936 PCF  64-129 374 G1944 AT-hook  87-100 376 G1946 HS 32-130 378 G1947 HS  37-120 380 G1948 AKR 75-126, 120-148, 152-181,186-215, 261-311, 312-363 382 G1950 AKR  65-228 384 G1958 GARP 230-278386 G2007 MYB-R1 R2R3  14-116 388 G2010 SBP  53-127 390 G2053 NAC 10-149 392 G2059 AP2 184-254 394 G2085 RING/C3HC4 214-241 396 G2105 TH100-153 398 G2110 WRKY 239-298 400 G2114 AP2 221-297, 323-393 402 G2117bZIP  46-106 404 G2123 GF14  99-109 406 G2130 AP2  93-160 408 G2133 AP211-83 410 G2138 AP2  76-148 412 G2140 HLH/MYC 167-242 414 G2143 HLH/MYC128-179 416 G2144 HLH/MYC 203-283 418 G2153 AT-hook 75-94, 162-206 420G2155 AT-hook 18-38 422 G2192 bZIP-NIN 600-700 424 G2295 MADS  2-57 426G2340 MYB-R1 R2R3  14-120 428 G2343 MYB-R1 R2R3  14-116 430 G2346 SBP 59-135 432 G2347 SBP  60-136 434 G2379 TH 19-110, 173-232 436 G2430GARP 425-478 438 G2505 NAC  10-159 440 G2509 AP2  89-156 442 G2517 WRKY118-174 444 G2520 HLH/MYC 135-206 446 G2555 HLH/MYC 175-245 448 G2557HLH/MYC 278-328 450 G2583 AP2  4-71 452 G2701 MYB-related 33-81, 129-183454 G2719 MYB-R1 R2R3  56-154 456 G2789 AT-hook 53-73, 121-165 458 G2830Z-C2H2 245-266

Examples of some of the utilities that may be desirable in plants, andthat may be provided by transforming the plants with the presentlydisclosed sequences, are listed in Table 6. Many of the transcriptionfactors listed in Table 6 may be operably linked with a specificpromoter that causes the transcription factor to be expressed inresponse to environmental, tissue-specific or temporal signals. Forexample, G362 induces ectopic trichomes on flowers but also producessmall plants. The former may be desirable to produce insect or herbivoreresistance, or increased cotton yield, but the latter may be undesirablein that it may reduce biomass. However, by operably linking G362 with aflower-specific promoter, one may achieve the desirable benefits of thegene without affecting overall biomass to a significant degree. Forexamples of flower specific promoters, see Kaiser et al. (supra). Forexamples of other tissue-specific, temporal-specific or induciblepromoters, see the above discussion under the heading “Vectors,Promoters, and Expression Systems”.

TABLE 6 Genes, traits and utilities that affect plant characteristicsTranscription factor genes Trait Category Phenotype(s) that impacttraits Utility Abiotic stress Effect of chilling on plants Increasedtolerance: G256; G664; G1322 Improved germination, growth rate, earlierplanting, yield Germination in cold Increased tolerance: G256; G664Earlier planting; improved survival, yield Freezing tolerance G720 (G720KO is more Earlier planting; susceptible); G912; G913 improved quality,survival, yield Drought Increased tolerance: G912; G913; G1820; G1836;Improved survival, G2505 vigor, appearance, yield Heat Increasedtolerance: G464; G682; G864; G1305; Improved germination, G1841; G2130;G2430 growth rate, later planting, yield Osmotic stress Increasedsensitivity: G1794 Abiotic stress response manipulation Increasedtolerance: G47; G175; G188; G303; G325; Improved germination G353; G489;G922; G926; rate, seedling vigor, G1069; G1089; G1452; G1816; survival,yield G1820; G1852; G1930; G2053; G2140; G2153; G2379; G2701; G2719;G2789; G2839 Salt tolerance More susceptible: G545 Manipulation ofresponse to high salt conditions Increased tolerance: G22; G196; G226;G312; G482; Improved germination G801; G867; G922; G1836; rate,survival, yield; G2110 extended growth range Nitrogen stress Sensitivityto N limitation: G1794 Manipulation of response to low nutrientconditions Tolerance to N limitation: G225; G226; G839; G1792; Improvedyield and G1816 nutrient stress tolerance, decreased fertilizer usagePhosphate stress Tolerance to P limitation: G545; G561; G911; G1946Improved yield and nutrient stress tolerance, decreased fertilizer usageOxidative stress G477 Improved yield, quality, ultraviolet and chemicalstress tolerance Herbicide Glyphosate G343; G2133; G2517 Generation ofglyphosate-resistant plants to improve weed control Hormone Abscisicacid (ABA) sensitivity sensitivity Reduced sensitivity to ABA: G546;G926; G1069; G1357; Modification of seed G1452; G1820; G2140; G2789development, improved seed dormancy, cold and dehydration toleranceSensitivity to ethylene Altered response: G1134 Manipulation of fruitripening Insensitive to ethylene: G1330 Disease Botrytis Increasedsusceptibility: G248; G371; G1064; G1084; Manipulation of G1196; G1255;G1756; G1936; response to disease G2555 organism Increased resistance orG28; G1792; G1880; G1919; Improved yield, tolerance: G1950 appearance,survival, extended range Fusarium Increased susceptibility: G188; G545;G896 Manipulation of response to disease organism Increased resistanceor G1047; G1792 Improved yield, tolerance: appearance, survival,extended range Erysiphe Increased susceptibility: G545; G881Manipulation of response to disease organism Increased resistance orG19; G28; G409; G1266; Improved yield, tolerance: G1363; G1792appearance, survival, extended range Pseudomonas Increasedsusceptibility: G545 Manipulation of response to disease organismSclerotinia Increased susceptibility: G278; G477; G594; G1936Manipulation of response to disease organism Increased resistance orG28; G1927 Improved yield, tolerance: appearance, survival, extendedrange Growth regulator Altered sugar sensing Decreased tolerance tosugars: G241; G254; G567; G680; Alteration of energy G912; G1804balance, photosynthetic Increased tolerance to sugars: G481; G867;G1225; G1816 rate, carbohydrate accumulation, biomass production,source-sink relationships, senescence; alteration of storage compoundaccumulation in seeds Altered C/N sensing G1816 Flowering time Earlyflowering G157; G180; G183; G485 (OE); Faster generation time; G490;G590; G789; G1225; synchrony of flowering; G1494; G1820; G1841; G1842;additional harvests G1843; G1946; G2010; G2144; within a growing season,G2295; G2347; G2509 shortening of breeding programs Late flowering G8;G47; G157; G192; G214; Increased yield or G231; G361; G362; G485 (KO);biomass, alleviate risk of G562; G736; G748; G859; transgenic pollenescape, G910; G913; G971; G1051; synchrony of flowering G1052; G1357;G1452; G1478; G1804; G1895; G1945; G2007; G2133; G2155; G2838 GeneralAltered flower structure development and Stamen: G988; G1075; G1140;G1499; Ornamental morphology G2557 modification of plant Sepal: G1075;G1140; G2557 architecture, improved Petal: G638; G1075; G1140; G1449; orreduced fertility to G1499; G2557 mitigate escape of Pedicel: G353;G354; G988 transgenic pollen, Carpel: G1063; G1140; G2143; G2143;improved fruit size, G2557 shape, number or yield Multiple alterations:G638; G988; G1063; G1140; G1449; G1499; G2143; G2557 G988; G1449; G2838Enlarged floral organs: G353; G354 Siliques: G470; G779; G988; G1075;G1140; G1499; G1947; G2143; G2557 Reduced fertility: G638; G779; G1140;G1499 Aerial rosettes G1995; G2826; G2838 Inflorescence architecturalchange Altered branching pattern: G47; G1063; G1645; G2143 OrnamentalShort internodes/bushy G47 modification of flower inflorescences:architecture; timing of Internode elongation: G1063 flowering; alteredplant Lack of inflorescence: G1499; G2143 habit for yield orharvestability benefit; reduction in pollen production of geneticallymodified plants; manipulation of seasonality and annual or perennialhabit; manipulation of determinate vs. indeterminate growth Alteredshoot meristem development Stem bifurcations: G390; G391 Ornamentalmodification of plant architecture, manipulation of growth anddevelopment, increase in leaf numbers, modulation of branching patternsto provide improved yield or biomass Altered branching pattern G427;G568; G988; G1543; Ornamental G1794 modification of plant architecture,improved lodging resistance Apical dominance Reduced apical dominance:G47; G211; G1255; G1275; Ornamental G1411; G1488; G1794; G2509modification of plant architecture Altered trichome density;development, or structure Reduced or no trichomes: G225; G226; G247;G585; Ornamental G676; G682; G1332; G1452; modification of plant G1816architecture, increased Ectopic trichomes/altered G247; G362; G370;G676; plant product (e.g., trichome development/cell G2826 diterpenes,cotton) fate: productivity, insect and Increase in trichome number,G362; G634; G838; G2838 herbivore resistance size or density: Stemmorphology and altered G47; G438; G748; G988; Modulation of ligninvascular tissue structure G1488 content; improvement of wood,palatability of fruits and vegetables Root development Increased rootgrowth and G1482 Improved yield, stress proliferation: tolerance;anchorage Increased root hairs: G225; G226; G1816 Altered seeddevelopment, G979 ripening and germination Cell differentiation and cellG1540 Increase in carpel or proliferation fruit development; improveregeneration of shoots from callus in transformation or micro-propagation systems Rapid development G2430 Promote faster developmentand reproduction in plants Senescence Premature senescence: G636; G1463;G1944 Improvement in response to disease, fruit ripening Lethality whenoverexpressed G877; G1519 Herbicide target; ablation of specific tissuesor organs such as stamen to prevent pollen escape Necrosis G12, G24Disease resistance Plant size Increased plant size G1073; G1451 Improvedyield, biomass, appearance Larger seedlings G2346; G2838 Increasedsurvival and vigor of seedlings, yield Dwarfed or more compact G24;G343; G353; G354; G362; Dwarfism, lodging plants G370; G1008; G1277;G1543; resistance, manipulation G1794; G1958 of gibberellin responsesLeaf morphology Dark green leaves G674; G912; G1063; G1357; IncreasedG1452; G1482; G1499; G1792; photosynthesis, biomass, G1863; G1888;G2143; G2557; appearance, yield G2838 Change in leaf shape G211; G353;G674; G736; Ornamental applications G1063; G1146; G1357; G1452; G1494;G1543; G1863; G2143; G2144 Altered leaf size: Increased leaf size,number or G189; G214; G1451; G2430 Increased yield, mass: ornamentalapplications Light green leaves G1494; G2144 Ornamental applicationsVariegation G635 Ornamental applications Glossy leaves G30; G1792; G2583Ornamental applications, manipulation of wax composition, amount, ordistribution Seed morphology Altered seed coloration G156; G2105; G2085Appearance Seed size and shape Increased seed size: G450; G584; G1255;G2085; Yield, appearance G2105; G2114 Decreased seed size: G1040Appearance Altered seed shape: G1040; G1062 Appearance Leaf biochemistryIncreased leaf wax G975; G1792; G2583 Insect, pathogen resistance Leafprenyl lipids Reduced chlorophyll: G987 Increase in tocopherols G652;G987; G2509 Increased lutein content G748 Increase in chlorophyll orG214; G1543 carotenoids: Leaf insoluble sugars Increase in leaf xyloseG211 Increased leaf anthocyanins G663; G1482; G1888 Leaf fatty acidsReduction in leaf fatty acids: G987 Increase in leaf fatty acids: G214Seed Seed oil content biochemistry Increased oil content: G162; G291;G427; G509; Improved oil yield G519; G561; G590; G598; Reduced caloriccontent G629; G715; G849; G961; G1198; G1226; G1471; G1478; G1526;G1640; G1646; G1750; G1765; G1777; G1793; G1838; G1902; G1946; G1948;G1958, G2123; G2138; G2343; G2830 Decreased oil content: G180; G192;G241; G504; G1143; G1229; G1323; G1543; G2509 Altered oil content: G567;G892; G974; G1451; G1496; G1646; G1672; G1677 Altered fatty acidcontent: G869; G1417; G2192 Seed protein content Increased proteincontent: G162; G226; G241; G509; Improved protein yield, G988; G1323;G1419; G1652; nutritional value G1818; G1820; G1958; G2117; Reducedcaloric content G2509 Decreased protein content: G427; G1478; G1777;G1903; G1946 Altered protein content: G162; G567; G597; G849; G892;G1634; G1637; G1677 Altered seed prenyl lipid G652; G2509; G2520Improved antioxidant content or composition and vitamin E content Seedglucosinolate Altered profile: G484; G2340 Increased seed anthocyaninsG362; G663 Root Increased root anthocyanins G663 Biochemistry LightAltered cotyledon, hypocotyl, G183; G354; G1322; G1331; Potential forincreased response/shade petiole development; altered G1488; G1494;G1794; G2144; planting densities and avoidance leaf orientation;constitutive G2555 yield enhancement photomorphogenesis;photomorphogenesis in low light Pigment Increased anthocyanin levelG362; G663; G1482 Enhanced health benefits, improved ornamentalappearance, increased stress resistance, attraction of pollinating andseed- dispersing animals Abbreviations: N = nitrogen P = phosphate ABA =abscisic acid C/N = carbon/nitrogen balanceDetailed Description of Genes, Traits and Utilities that Affect PlantCharacteristics

The following descriptions of traits and utilities associated with thepresent transcription factors offer a more comprehensive descriptionthan that provided in Table 6.

Abiotic Stress, General Considerations

Plant transcription factors can modulate gene expression, and, in turn,be modulated by the environmental experience of a plant. Significantalterations in a plant's environment invariably result in a change inthe plant's transcription factor gene expression pattern. Alteredtranscription factor expression patterns generally result in phenotypicchanges in the plant. Transcription factor gene product(s) in transgenicplants then differ(s) in amounts or proportions from that found inwild-type or non-transformed plants, and those transcription factorslikely represent polypeptides that are used to alter the response to theenvironmental change. By way of example, it is well accepted in the artthat analytical methods based on altered expression patterns may be usedto screen for phenotypic changes in a plant far more effectively thancan be achieved using traditional methods.

Abiotic Stress: Adult Stage Chilling.

Enhanced chilling tolerance may extend the effective growth range ofchilling sensitive crop species by allowing earlier planting or laterharvest. Improved chilling tolerance may be conferred by increasedexpression of glycerol-3-phosphate acetyltransferase in chloroplasts(see, for example, Wolter et al. (1992) et al. EMBO J. 4685-4692, andMurata et al. (1992) Nature 356: 710-713).

Chilling tolerance could also serve as a model for understanding howplants adapt to water deficit. Both chilling and water stress sharesimilar signal transduction pathways and tolerance/adaptationmechanisms. For example, acclimation to chilling temperatures can beinduced by water stress or treatment with abscisic acid. Genes inducedby low temperature include dehydrins (or LEA proteins). Dehydrins arealso induced by salinity, abscisic acid, water stress, and during thelate stages of embryogenesis.

Another large impact of chilling occurs during post-harvest storage. Forexample, some fruits and vegetables do not store well at lowtemperatures (for example, bananas, avocados, melons, and tomatoes). Thenormal ripening process of the tomato is impaired if it is exposed tocool temperatures. Transcription factor genes conferring resistance tochilling temperatures, including G256, G664, and G1322 may thus enhancetolerance during post-harvest storage.

Abiotic Stress: Cold Germination.

Several of the presently disclosed transcription factor genes conferbetter germination and growth in cold conditions. For example, theimproved germination in cold conditions seen with G256 and G664indicates a role in regulation of cold responses by these genes andtheir equivalogs. These genes might be engineered to manipulate theresponse to low temperature stress. Genes that would allow germinationand seedling vigor in the cold would have highly significant utility inallowing seeds to be planted earlier in the season with a high rate ofsurvival. Transcription factor genes that confer better survival incooler climates allow a grower to move up planting time in the springand extend the growing season further into autumn for higher cropyields. Germination of seeds and survival at temperatures significantlybelow that of the mean temperature required for germination of seeds andsurvival of non-transformed plants would increase the potential range ofa crop plant into regions in which it would otherwise fail to thrive.

Abiotic Stress: Freezing Tolerance and Osmotic Stress.

Presently disclosed transcription factor genes, including G47, G175,G188, G303, G325, G353, G489, G922, G926, G1069, G1089, G1452, G1820,G1852, G1930, G2053, G2140, G2153, G2379, G2701, G2719, G2789, G2839 andtheir equivalogs, that increase germination rate and/or growth underadverse osmotic conditions, could impact survival and yield of seeds andplants. Osmotic stresses may be regulated by specific molecular controlmechanisms that include genes controlling water and ion movements,functional and structural stress-induced proteins, signal perception andtransduction, and free radical scavenging, and many others (Wang et al.(2001) Acta Hort. (ISHS) 560: 285-292). Instigators of osmotic stressinclude freezing, drought and high salinity, each of which are discussedin more detail below.

In many ways, freezing, high salt and drought have similar effects onplants, not the least of which is the induction of common polypeptidesthat respond to these different stresses. For example, freezing issimilar to water deficit in that freezing reduces the amount of wateravailable to a plant. Exposure to freezing temperatures may lead tocellular dehydration as water leaves cells and forms ice crystals inintercellular spaces (Buchanan, supra). As with high salt concentrationand freezing, the problems for plants caused by low water availabilityinclude mechanical stresses caused by the withdrawal of cellular water.Thus, the incorporation of transcription factors that modify a plant'sresponse to osmotic stress or improve tolerance to (e.g., by G720, G912,G913 or their equivalogs) into, for example, a crop or ornamental plant,may be useful in reducing damage or loss. Specific effects caused byfreezing, high salt and drought are addressed below.

Abiotic Stress: Drought and Low Humidity Tolerance.

Exposure to dehydration invokes similar survival strategies in plants asdoes freezing stress (see, for example, Yelenosky (1989) Plant Physiol89: 444-451) and drought stress induces freezing tolerance (see, forexample, Siminovitch et al. (1982) Plant Physiol 69: 250-255; and Guy etal. (1992) Planta 188: 265-270). In addition to the induction ofcold-acclimation proteins, strategies that allow plants to survive inlow water conditions may include, for example, reduced surface area, orsurface oil or wax production. A number of presently disclosedtranscription factor genes, e.g., G912, G913, G1820, G1836 and G2505increase a plant's tolerance to low water conditions and, along withtheir functional equivalogs, would provide the benefits of improvedsurvival, increased yield and an extended geographic and temporalplanting range.

Abiotic Stress: Heat Stress Tolerance.

The germination of many crops is also sensitive to high temperatures.Presently disclosed transcription factor genes that provide increasedheat tolerance, including G464, G682, G864, G1305, G1841, G2130, G2430and their equivalogs, would be generally useful in producing plants thatgerminate and grow in hot conditions, may find particular use for cropsthat are planted late in the season, or extend the range of a plant byallowing growth in relatively hot climates.

Abiotic Stress: Salt.

The genes in Table 6 that provide tolerance to salt may be used toengineer salt tolerant crops and trees that can flourish in soils withhigh saline content or under drought conditions. In particular,increased salt tolerance during the germination stage of a plantenhances survival and yield. Presently disclosed transcription factorgenes, including G22, G196, G226, G312, G482, G801, G867, G922, G1836,G2110, and their equivalogs that provide increased salt tolerance duringgermination, the seedling stage, and throughout a plant's life cycle,would find particular value for imparting survival and yield in areaswhere a particular crop would not normally prosper.

Nutrient Uptake and Utilization: Nitrogen and Phosphorus.

Presently disclosed transcription factor genes introduced into plantsprovide a means to improve uptake of essential nutrients, includingnitrogenous compounds, phosphates, potassium, and trace minerals. Theenhanced performance of, for example, G225, G226, G839, G1792, and otheroverexpressing lines under low nitrogen, and G545, G561, G911, G1946under low phosphorous conditions indicate that these genes and theirequivalogs can be used to engineer crops that could thrive underconditions of reduced nutrient availability. Phosphorus, in particular,tends to be a limiting nutrient in soils and is generally added as acomponent in fertilizers. Young plants have a rapid intake of phosphateand sufficient phosphate is important for yield of root crops such ascarrot, potato and parsnip.

The effect of these modifications is to increase the seedlinggermination and range of ornamental and crop plants. The utilities ofpresently disclosed transcription factor genes conferring tolerance toconditions of low nutrients also include cost savings to the grower byreducing the amounts of fertilizer needed, environmental benefits ofreduced fertilizer runoff into watersheds; and improved yield and stresstolerance. In addition, by providing improved nitrogen uptakecapability, these genes can be used to alter seed protein amounts and/orcomposition in such a way that could impact yield as well as thenutritional value and production of various food products.

A number of the transcription factor-overexpressing lines make lessanthocyanin on high sucrose plus glutamine indicates that these genescan be used to modify carbon and nitrogen status, and hence assimilatepartitioning (assimilate partitioning refers to the manner in which anessential element, such as nitrogen, is distributed among differentpools inside a plant, generally in a reduced form, for the purpose oftransport to various tissues).

Increased Tolerance of Plants to Oxidative Stress.

In plants, as in all living things, abiotic and biotic stresses inducethe formation of oxygen radicals, including superoxide and peroxideradicals. This has the effect of accelerating senescence, particularlyin leaves, with the resulting loss of yield and adverse effect onappearance. Generally, plants that have the highest level of defensemechanisms, such as, for example, polyunsaturated moieties of membranelipids, are most likely to thrive under conditions that introduceoxidative stress (e.g., high light, ozone, water deficit, particularlyin combination). Introduction of the presently disclosed transcriptionfactor genes, including G477 and its equivalogs, that increase the levelof oxidative stress defense mechanisms would provide beneficial effectson the yield and appearance of plants. One specific oxidizing agent,ozone, has been shown to cause significant foliar injury, which impactsyield and appearance of crop and ornamental plants. In addition toreduced foliar injury that would be found in ozone resistant plantcreated by transforming plants with some of the presently disclosedtranscription factor genes, the latter have also been shown to haveincreased chlorophyll fluorescence (Yu-Sen Chang et al. (2001) Bot.Bull. Acad. Sin. 42: 265-272).

Decreased Herbicide Sensitivity.

Presently disclosed transcription factor genes, including G343, G2133,G2517 and their equivalogs, that confer resistance or tolerance toherbicides (e.g., glyphosate) will find use in providing means toincrease herbicide applications without detriment to desirable plants.This would allow for the increased use of a particular herbicide in alocal environment, with the effect of increased detriment to undesirablespecies and less harm to transgenic, desirable cultivars.

Knockouts of a number of the presently disclosed transcription factorgenes have been shown to be lethal to developing embryos. Thus, thesegenes are potentially useful as herbicide targets.

Hormone Sensitivity.

ABA plays regulatory roles in a host of physiological processes in allhigher as well as in lower plants (Davies et al. (1991) Abscisic Acid:Physiology and Biochemistry. Bios Scientific Publishers, Oxford, UK;Zeevaart et al. (1988) Ann Rev Plant Physiol. Plant Mol. Biol. 49:439-473; Shimizu-Sato et al. (2001) Plant Physiol 127: 1405-1413). ABAmediates stress tolerance responses in higher plants, is a key signalcompound that regulates stomatal aperture and, in concert with otherplant signaling compounds, is implicated in mediating responses topathogens and wounding or oxidative damage (for example, see Larkindaleet al. (2002) Plant Physiol. 128: 682-695). In seeds, ABA promotes seeddevelopment, embryo maturation, synthesis of storage products (proteinsand lipids), desiccation tolerance, and is involved in maintenance ofdormancy (inhibition of germination), and apoptosis (Zeevaart et al.(1988) Ann Rev Plant Physiol. Plant Mol. Biol. 49: 439-473; Davies(1991), supra; Thomas (1993) Plant Cell 5: 1401-1410; and Bethke et al.(1999) Plant Cell 11: 1033-1046). ABA also affects plant architecture,including root growth and morphology and root-to-shoot ratios. ABAaction and metabolism is modulated not only by environmental signals butalso by endogenous signals generated by metabolic feedback, transport,hormonal cross-talk and developmental stage. Manipulation of ABA levels,and hence by extension the sensitivity to ABA, has been described as avery promising means to improve productivity, performance andarchitecture in plants Zeevaart (1999) in: Biochemistry and MolecularBiology of Plant Hormones, Hooykaas et al. eds, Elsevier Science pp189-207; and Cutler et al. (1999) Trends Plant Sci. 4: 472-478).

A number of the presently disclosed transcription factor genes affectplant abscisic acid (ABA) sensitivity, including G546, G926, 1069,G1357, G1452, G1820, G2140, G2789. Thus, by affecting ABA sensitivity,these introduced transcription factor genes and their equivalogs wouldaffect cold, drought, oxidative and other stress sensitivities, plantarchitecture, and yield.

Several other of the present transcription factor genes have been usedto manipulate ethylene signal transduction and response pathways. Thesegenes can thus be used to manipulate the processes influenced byethylene, such as seed germination or fruit ripening, and to improveseed or fruit quality.

Diseases, Pathogens and Pests.

A number of the presently disclosed transcription factor genes have beenshown to or are likely to affect a plants response to various plantdiseases, pathogens and pests. The offending organisms include fungalpathogens Fusarium oxysporum, Botrytis cinerea, Sclerotiniasclerotiorum, and Erysiphe orontii. Bacterial pathogens to whichresistance may be conferred include Pseudomonas syringae. Other problemorganisms may potentially include nematodes, mollicutes, parasites, orherbivorous arthropods. In each case, one or more transformedtranscription factor genes may provide some benefit to the plant to helpprevent or overcome infestation, or be used to manipulate any of thevarious plant responses to disease. These mechanisms by which thetranscription factors work could include increasing surface waxes oroils, surface thickness, or the activation of signal transductionpathways that regulate plant defense in response to attacks byherbivorous pests (including, for example, protease inhibitors). Anothermeans to combat fungal and other pathogens is by accelerating local celldeath or senescence, mechanisms used to impair the spread of pathogenicmicroorganisms throughout a plant. For instance, the best known exampleof accelerated cell death is the resistance gene-mediated hypersensitiveresponse, which causes localized cell death at an infection site andinitiates a systemic defense response. Because many defenses, signalingmolecules, and signal transduction pathways are common to defenseagainst different pathogens and pests, such as fungal, bacterial,oomycete, nematode, and insect, transcription factors that areimplicated in defense responses against the fungal pathogens tested mayalso function in defense against other pathogens and pests. Thesetranscription factors include, for example, G28, G1792, G1880, G1919,G1950 (improved resistance or tolerance to Botrytis), G1047, G1792(improved resistance or tolerance to Fusarium), G19, G28, G409, G1266,G1363, G1792 (improved resistance or tolerance to Erysiphe), G545(improved resistance or tolerance to Pseudomonas), G28, G1927 (improvedresistance or tolerance to Sclerotinia), and their equivalogs.

Growth Regulator: Sugar Sensing.

In addition to their important role as an energy source and structuralcomponent of the plant cell, sugars are central regulatory moleculesthat control several aspects of plant physiology, metabolism anddevelopment (Hsieh et al. (1998) Proc. Natl. Acad. Sci. 95:13965-13970). It is thought that this control is achieved by regulatinggene expression and, in higher plants, sugars have been shown to repressor activate plant genes involved in many essential processes such asphotosynthesis, glyoxylate metabolism, respiration, starch and sucrosesynthesis and degradation, pathogen response, wounding response, cellcycle regulation, pigmentation, flowering and senescence. The mechanismsby which sugars control gene expression are not understood.

Because sugars are important signaling molecules, the ability to controleither the concentration of a signaling sugar or how the plant perceivesor responds to a signaling sugar could be used to control plantdevelopment, physiology or metabolism. For example, the flux of sucrose(a disaccharide sugar used for systemically transporting carbon andenergy in most plants) has been shown to affect gene expression andalter storage compound accumulation in seeds. Manipulation of thesucrose signaling pathway in seeds may therefore cause seeds to havemore protein, oil or carbohydrate, depending on the type ofmanipulation. Similarly, in tubers, sucrose is converted to starch whichis used as an energy store. It is thought that sugar signaling pathwaysmay partially determine the levels of starch synthesized in the tubers.The manipulation of sugar signaling in tubers could lead to tubers witha higher starch content.

Thus, the presently disclosed transcription factor genes that manipulatethe sugar signal transduction pathway, including G241, G254, G567, G680,G912, G1804, G481, G867, G1225, along with their equivalogs, may lead toaltered gene expression to produce plants with desirable traits. Inparticular, manipulation of sugar signal transduction pathways could beused to alter source-sink relationships in seeds, tubers, roots andother storage organs leading to increase in yield.

Growth Regulator: C/N Sensing.

Nitrogen and carbon metabolism are tightly linked in almost everybiochemical pathway in the plant. Carbon metabolites regulate genesinvolved in N acquisition and metabolism, and are known to affectgermination and the expression of photosynthetic genes (Coruzzi et al.(2001) Plant Physiol. 125: 61-64) and hence growth. Early studies onnitrate reductase (NR) in 1976 showed that NR activity could be affectedby Glc/Suc (Crawford (1995) Plant Cell 7: 859-886; Daniel-Vedele et al.(1996) CR Acad Sci Paris 319: 961-968). Those observations weresupported by later experiments that showed sugars induce NR mRNA indark-adapted, green seedlings (Cheng C L, et al. (1992) Proc Natl AcadSci USA 89: 1861-1864). C and N may have antagonistic relationships assignaling molecules; light induction of NR activity and mRNA levels canbe mimicked by C metabolites and N-metabolites cause repression of NRinduction in tobacco (Vincentz et al. (1992) Plant J 3: 315-324). Generegulation by C/N status has been demonstrated for a number ofN-metabolic genes (Stitt (1999) Curr. Opin. Plant. Biol. 2: 178-186);Coruzzi et al. (2001) supra). Thus, transcription factor genes thataffect C/N sensing, such as G1816, can be used to alter or improvegermination and growth under nitrogen-limiting conditions.

Flowering Time: Early and Late Flowering.

Presently disclosed transcription factor genes that accelerateflowering, which include G157, G180, G183, G485, G490, G590, G789,G1225, G1494, G1820, G1841, G1842, G1843, G1946, G2010, G2144, G2295,G2347, G2509, and their functional equivalogs, could have valuableapplications in such programs, since they allow much faster generationtimes. In a number of species, for example, broccoli, cauliflower, wherethe reproductive parts of the plants constitute the crop and thevegetative tissues are discarded, it would be advantageous to acceleratetime to flowering. Accelerating flowering could shorten crop and treebreeding programs. Additionally, in some instances, a faster generationtime would allow additional harvests of a crop to be made within a givengrowing season. A number of Arabidopsis genes have already been shown toaccelerate flowering when constitutively expressed. These include LEAFY,APETALA1 and CONSTANS (Mandel et al. (1995) Nature 377: 522-524; Weigeland Nilsson (1995) Nature 377: et al. 495-500; Simon et al. (1996)Nature 384: 59-62).

By regulating the expression of potential flowering using induciblepromoters, flowering could be triggered by application of an inducerchemical. This would allow flowering to be synchronized across a cropand facilitate more efficient harvesting. Such inducible systems couldalso be used to tune the flowering of crop varieties to differentlatitudes. At present, species such as soybean and cotton are availableas a series of maturity groups that are suitable for different latitudeson the basis of their flowering time (which is governed by day-length).A system in which flowering could be chemically controlled would allow asingle high-yielding northern maturity group to be grown at anylatitude. In southern regions such plants could be grown for longerperiods before flowering was induced, thereby increasing yields. In morenorthern areas, the induction would be used to ensure that the cropflowers prior to the first winter frosts.

In a sizeable number of species, for example, root crops, where thevegetative parts of the plants constitute the crop and the reproductivetissues are discarded, it is advantageous to identify and incorporatetranscription factor genes that delay or prevent flowering in order toprevent resources being diverted into reproductive development. Forexample, G8, G47, G157, G192, G214, G231; G361, G362, G562, G736, G748,G859, G910, G913, G971, G1051, G1052, G1357, G1452, G1478, G1804, G1895,G1945, G2007, G2133, G2155, G2838 and equivalogs, delay flowering timein transgenic plants. Extending vegetative development with presentlydisclosed transcription factor genes could thus bring about largeincreases in yields. Prevention of flowering can help maximizevegetative yields and prevent escape of genetically modified organism(GMO) pollen.

Presently disclosed transcription factors that extend flowering timehave utility in engineering plants with longer-lasting flowers for thehorticulture industry, and for extending the time in which the plant isfertile.

A number of the presently disclosed transcription factors may extendflowering time, and delay flower abscission, which would have utility inengineering plants with longer-lasting flowers for the horticultureindustry. This would provide a significant benefit to the ornamentalindustry, for both cut flowers and woody plant varieties (of, forexample, maize), as well as have the potential to lengthen the fertileperiod of a plant, which could positively impact yield and breedingprograms.

General Development and Morphology: Flower Structure and Inflorescence:Architecture, Altered Flower Organs, Reduced Fertility, MultipleAlterations, Aerial Rosettes, Branching, Internode Distance, TerminalFlowers and Phase Change.

Presently disclosed transgenic transcription factors such as G353; G354,G638; G779; G988; G1063; G1075; G1140; G1449; G1499; G2143; G2557,G2838, G2839 and their equivalogs, may be used to create plants withlarger flowers or arrangements of flowers that are distinct fromwild-type or non-transformed cultivars. This would likely have the mostvalue for the ornamental horticulture industry, where larger flowers orinteresting floral configurations are generally preferred and commandthe highest prices.

Flower structure may have advantageous or deleterious effects onfertility, and could be used, for example, to decrease fertility by theabsence, reduction or screening of reproductive components. In fact,plants that overexpress a sizable number of the presently disclosedtranscription factor genes e.g., G470, G779, G988, G1075, G1140, G1499,G1947, G2143, G2557 and their functional equivalogs, possess reducedfertility; flowers are infertile and fail to yield seed. These could bedesirable traits, as low fertility could be exploited to prevent orminimize the escape of the pollen of genetically modified organisms(GMOs) into the environment.

The alterations in shoot architecture seen in the lines transformed withG47, G1063, G1645, G2143, and their functional equivalogs indicates thatthese genes and their equivalogs can be used to manipulate inflorescencebranching patterns. This could influence yield and offer the potentialfor more effective harvesting techniques. For example, a “self pruning”mutation of tomato results in a determinate growth pattern andfacilitates mechanical harvesting (Pnueli et al. (2001) Plant Cell13(12): 2687-702).

One interesting application for manipulation of flower structure, forexample, by introduced transcription factors could be in the increasedproduction of edible flowers or flower parts, including saffron, whichis derived from the stigmas of Crocus sativus.

Genes that later silique conformation in brassicates may be used tomodify fruit ripening processes in brassicates and other plants, whichmay positively affect seed or fruit quality.

A number of the presently disclosed transcription factors may affect thetiming of phase changes in plants. Since the timing or phase changesgenerally affects a plant's eventual size, these genes may provebeneficial by providing means for improving yield and biomass.

General Development and Morphology: Shoot Meristem and BranchingPatterns.

Several of the presently disclosed transcription factor genes, includingG390 and G391, and G1794, when introduced into plants, have been shownto cause stem bifurcations in developing shoots in which the shootmeristems split to form two or three separate shoots. Thesetranscription factors and their functional equivalogs may thus be usedto manipulate branching. This would provide a unique appearance, whichmay be desirable in ornamental applications, and may be used to modifylateral branching for use in the forestry industry. A reduction in theformation of lateral branches could reduce knot formation. Conversely,increasing the number of lateral branches could provide utility when aplant is used as a view- or windscreen.

General Development and Morphology: Apical Dominance:

The modified expression of presently disclosed transcription factors(e.g., G47, G211, G1255, G1275, G1411, G1488, G1794, G2509 and theirequivalogs) that reduce apical dominance could be used in ornamentalhorticulture, for example, to modify plant architecture, for example, toproduce a shorter, more bushy stature than wild type. The latter formwould have ornamental utility as well as provide increased resistance tolodging.

General Development and Morphology: Trichome Density, Development orStructure.

Several of the presently disclosed transcription factor genes have beenused to modify trichome number, density, trichome cell fate, amount oftrichome products produced by plants, or produce ectopic trichomeformation. These include G225; G226, G247; G362, G370; G585, G634, G676,G682, G1332, G1452, G1995, G2826, and G2838. In most cases where themetabolic pathways are impossible to engineer, increasing trichomedensity or size on leaves may be the only way to increase plantproductivity. Thus, by increasing trichome density, size or type, thesetrichome-affecting genes and their functional equivalogs would haveprofound utilities in molecular farming practices by making use oftrichomes as a manufacturing system for complex secondary metabolites.

Trichome glands on the surface of many higher plants produce and secreteexudates that give protection from the elements and pests such asinsects, microbes and herbivores. These exudates may physicallyimmobilize insects and spores, may be insecticidal or ant-microbial orthey may act as allergens or irritants to protect against herbivores. Bymodifying trichome location, density or activity with presentlydisclosed transcription factors that modify these plant characteristics,plants that are better protected and higher yielding may be the result.

A potential application for these trichome-affecting genes and theirequivalogs also exists in cotton: cotton fibers are modified unicellulartrichomes that develop from the outer ovule epidermis. In fact, onlyabout 30% of these epidermal cells develop into trichomes, but all havethe potential to develop a trichome fate. Trichome-affecting genes cantrigger an increased number of these cells to develop as trichomes andthereby increase the yield of cotton fibers. Since the mallow family isclosely related to the Brassica family, genes involved in trichomeformation will likely have homologs in cotton or function in cotton.

If the effects on trichome patterning reflect a general change inheterochronic processes, trichome-affecting transcription factors ortheir equivalogs can be used to modify the way meristems and/or cellsdevelop during different phases of the plant life cycle. In particular,altering the timing of phase changes could afford positive effects onyield and biomass production.

General Development and Morphology: Stem Morphology and Altered VascularTissue Structure.

Plants transformed with transcription factor genes that modify stemmorphology or lignin content may be used to affect overall plantarchitecture and the distribution of lignified fiber cells within thestem.

Modulating lignin content might allow the quality of wood used forfurniture or construction to be improved. Lignin is energy rich;increasing lignin composition could therefore be valuable in raising theenergy content of wood used for fuel. Conversely, the pulp and paperindustries seek wood with a reduced lignin content. Currently, ligninmust be removed in a costly process that involves the use of manypolluting chemicals. Consequently, lignin is a serious barrier toefficient pulp and paper production (Tzfira et al. (1998) TIBTECH 16:439-446; Robinson (1999) Nature Biotechnology 17: 27-30). In addition toforest biotechnology applications, changing lignin content byselectively expressing or repressing transcription factors in fruits andvegetables might increase their palatability.

Transcription factors that modify stem structure, including G47, G438,G748, G988, G1488 and their equivalogs, may also be used to achievereduction of higher-order shoot development, resulting in significantplant architecture modification. Overexpression of the genes that encodethese transcription factors in woody plants might result in trees thatlack side branches, and have fewer knots in the wood. Altering branchingpatterns could also have applications amongst ornamental andagricultural crops. For example, applications might exist in any specieswhere secondary shoots currently have to be removed manually, or wherechanges in branching pattern could increase yield or facilitate moreefficient harvesting.

General Development and Morphology: Altered Root Development.

By modifying the structure or development of roots by transforming intoa plant one or more of the presently disclosed transcription factorgenes, including G225, G226, G1482, and their equivalogs, plants may beproduced that have the capacity to thrive in otherwise unproductivesoils. For example, grape roots extending further into rocky soils wouldprovide greater anchorage, greater coverage with increased branching, orwould remain viable in waterlogged soils, thus increasing the effectiveplanting range of the crop and/or increasing yield and survival. It maybe advantageous to manipulate a plant to produce short roots, as when asoil in which the plant will be growing is occasionally flooded, or whenpathogenic fungi or disease-causing nematodes are prevalent.

General Development and Morphology: Seed Development, Ripening andGermination Rate.

A number of the presently disclosed transcription factor genes (e.g.,G979) have been shown to modify seed development and germination rate,including when the seeds are in conditions normally unfavorable forgermination (e.g., cold, heat or salt stress, or in the presence ofABA), and may, along with functional equivalogs, thus be used to modifyand improve germination rates under adverse conditions.

General Development and Morphology: Cell Differentiation and CellProliferation.

Several of the disclosed transcription factors regulate cellproliferation and/or differentiation, including G1540 and its functionalequivalogs. Control of these processes could have valuable applicationsin plant transformation, cell culture or micro-propagation systems, aswell as in control of the proliferation of particular useful tissues orcell types. Transcription factors that induce the proliferation ofundifferentiated cells can be operably linked with an inducible promoterto promote the formation of callus that can be used for transformationor production of cell suspension cultures. Transcription factors thatprevent cells from differentiating, such as G1540 or its equivalogs,could be used to confer stem cell identity to cultured cells.Transcription factors that promote differentiation of shoots could beused in transformation or micro-propagation systems, where regenerationof shoots from callus is currently problematic. In addition,transcription factors that regulate the differentiation of specifictissues could be used to increase the proportion of these tissues in aplant. Genes that promote the differentiation of carpel tissue could beintroduced into commercial species to induce formation of increasednumbers of carpels or fruits. A particular application might exist insaffron, one of the world's most expensive spices. Saffron filaments, orthreads, are actually the dried stigmas of the saffron flower, Crocussativus Linneaus. Each flower contains only three stigmas, and more than75,000 of these flowers are needed to produce just one pound of saffronfilaments. An increase in carpel number would increase the quantity ofstigmatic tissue and improve yield.

General Development and Morphology: Cell Expansion.

Plant growth results from a combination of cell division and cellexpansion. Transcription factors may be useful in regulation of cellexpansion. Altered regulation of cell expansion could affect stemlength, an important agronomic characteristic. For instance, shortcultivars of wheat contributed to the Green Revolution, because plantsthat put fewer resources into stem elongation allocate more resourcesinto developing seed and produce higher yield. These plants are alsoless vulnerable to wind and rain damage. These cultivars were found tobe altered in their sensitivity to gibberellins, hormones that regulatestem elongation through control of both cell expansion and celldivision. Altered cell expansion in leaves could also produce novel andornamental plant forms.

General Development and Morphology: Phase Change and Floral Reversion.

Transcription factors that regulate phase change can modulate thedevelopmental programs of plants and regulate developmental plasticityof the shoot meristem. In particular, these genes might be used tomanipulate seasonality and influence whether plants display an annual orperennial habit.

General Development and Morphology: Rapid Development.

A number of the presently disclosed transcription factor genes,including G2430, have been shown to have significant effects on plantgrowth rate and development. These observations have included, forexample, more rapid or delayed growth and development of reproductiveorgans. Thus, by causing more rapid development, G2430 and itsfunctional equivalogs would prove useful for regions with short growingseasons; other transcription factors that delay development may beuseful for regions with longer growing seasons. Accelerating plantgrowth would also improve early yield or increase biomass at an earlierstage, when such is desirable (for example, in producing forestryproducts or vegetable sprouts for consumption). Transcription factorsthat promote faster development such as G2430 and its functionalequivalogs may also be used to modify the reproductive cycle of plants.

General Development and Morphology: Slow Growth Rate.

A number of the presently disclosed transcription factor genes,including G652 and G1335, have been shown to have significant effects onretarding plant growth rate and development. These observations haveincluded, for example, delayed growth and development of reproductiveorgans. Slow growing plants may be highly desirable to ornamentalhorticulturists, both for providing house plants that display littlechange in their appearance over time, or outdoor plants for whichwild-type or rapid growth is undesirable (e.g., ornamental palm trees).Slow growth may also provide for a prolonged fruiting period, thusextending the harvesting season, particularly in regions with longgrowing seasons. Slow growth could also provide a prolonged period inwhich pollen is available for improved self- or cross-fertilization, orcross-fertilization of cultivars that normally flower overnon-overlapping time periods. The latter aspect may be particularlyuseful to plants comprising two or more distinct grafted cultivars(e.g., fruit trees) with normally non-overlapping flowering periods.

General Development and Morphology: Senescence.

Presently disclosed transcription factor genes may be used to altersenescence responses in plants. Although leaf senescence is thought tobe an evolutionary adaptation to recycle nutrients, the ability tocontrol senescence in an agricultural setting has significant value. Forexample, a delay in leaf senescence in some maize hybrids is associatedwith a significant increase in yields and a delay of a few days in thesenescence of soybean plants can have a large impact on yield. In anexperimental setting, tobacco plants engineered to inhibit leafsenescence had a longer photosynthetic lifespan, and produced a 50%increase in dry weight and seed yield (Gan and Amasino (1995) Science270: 1986-1988). Delayed flower senescence caused by overexpression oftranscription factors may generate plants that retain their blossomslonger and this may be of potential interest to the ornamentalhorticulture industry, and delayed foliar and fruit senescence couldimprove post-harvest shelf-life of produce.

Premature senescence caused by, for example, G636, G1463, G1944 andtheir equivalogs may be used to improve a plant's response to diseaseand hasten fruit ripening.

Growth Rate and Development: Lethality and Necrosis.

Overexpression of transcription factors, for example, G12, G24, G877,G1519 and their equivalogs that have a role in regulating cell death maybe used to induce lethality in specific tissues or necrosis in responseto pathogen attack. For example, if a transcription factor gene inducinglethality or necrosis was specifically active in gametes or reproductiveorgans, its expression in these tissues would lead to ablation andsubsequent male or female sterility. Alternatively, underpathogen-regulated expression, a necrosis-inducing transcription factorcan restrict the spread of a pathogen infection through a plant.

Plant Size: Large Plants.

Plants overexpressing G1073 and G1451, for example, have been shown tobe larger than controls. For some ornamental plants, the ability toprovide larger varieties with these genes or their equivalogs may behighly desirable. For many plants, including fruit-bearing trees, treesthat are used for lumber production, or trees and shrubs that serve asview or wind screens, increased stature provides improved benefits inthe forms of greater yield or improved screening. Crop species may alsoproduce higher yields on larger cultivars, particularly those in whichthe vegetative portion of the plant is edible.

Plant Size: Large Seedlings.

Presently disclosed transcription factor genes, that produce largeseedlings can be used to produce crops that become established faster.Large seedlings are generally hardier, less vulnerable to stress, andbetter able to out-compete weed species. Seedlings transformed withpresently disclosed transcription factors, including G2346 and G2838,for example, have been shown to possess larger cotyledons and were moredevelopmentally advanced than control plants. Rapid seedling developmentmade possible by manipulating expression of these genes or theirequivalogs is likely to reduce loss due to diseases particularlyprevalent at the seedling stage (e.g., damping off) and is thusimportant for survivability of plants germinating in the field or incontrolled environments.

Plant Size: Dwarfed Plants.

Presently disclosed transcription factor genes, including G24; G343,G353, G354, G362, G370; G1008, G1277, G1543, G1794, G1958 and theirequivalogs, for example, that can be used to decrease plant stature arelikely to produce plants that are more resistant to damage by wind andrain, have improved lodging resistance, or more resistant to heat or lowhumidity or water deficit. Dwarf plants are also of significant interestto the ornamental horticulture industry, and particularly for homegarden applications for which space availability may be limited.

Plant Size: Fruit Size and Number.

Introduction of presently disclosed transcription factor genes thataffect fruit size will have desirable impacts on fruit size and number,which may comprise increases in yield for fruit crops, or reduced fruityield, such as when vegetative growth is preferred (e.g., with bushyornamentals, or where fruit is undesirable, as with ornamental olivetrees).

Leaf Morphology: Dark Leaves.

Color-affecting components in leaves include chlorophylls (generallygreen), anthocyanins (generally red to blue) and carotenoids (generallyyellow to red). Transcription factor genes that increase these pigmentsin leaves, including G674, G912, G1063, G1357, G1452, G1482, G1499,G1792, G1863, G1888, G2143, G2557, G2838 and their equivalogs, maypositively affect a plant's value to the ornamental horticultureindustry. Variegated varieties, in particular, would show improvedcontrast. Other uses that result from overexpression of transcriptionfactor genes include improvements in the nutritional value offoodstuffs. For example, lutein is an important nutraceutical;lutein-rich diets have been shown to help prevent age-related maculardegeneration (ARMD), the leading cause of blindness in elderly people.Consumption of dark green leafy vegetables has been shown in clinicalstudies to reduce the risk of ARMD.

Enhanced chlorophyll and carotenoid levels could also improve yield incrop plants. Lutein, like other xanthophylls such as zeaxanthin andviolaxanthin, is an essential component in the protection of the plantagainst the damaging effects of excessive light. Specifically, luteincontributes, directly or indirectly, to the rapid rise ofnon-photochemical quenching in plants exposed to high light. Crop plantsengineered to contain higher levels of lutein could therefore haveimproved photo-protection, leading to less oxidative damage and bettergrowth under high light (e.g., during long summer days, or at higheraltitudes or lower latitudes than those at which a non-transformed plantwould survive). Additionally, elevated chlorophyll levels increasesphotosynthetic capacity.

Leaf Morphology: Changes in Leaf Shape.

Presently disclosed transcription factors produce marked and diverseeffects on leaf development and shape. The transcription factors includeG211, G353, G674, G736, G1063, G1146, G1357, G1452, G1494, G1543, G1863,G2143, G2144, and their equivalogs. At early stages of growth,transgenic seedlings have developed narrow, upward pointing leaves withlong petioles, possibly indicating a disruption in circadian-clockcontrolled processes or nyctinastic movements. Other transcriptionfactor genes can be used to alter leaf shape in a significant mannerfrom wild type, some of which may find use in ornamental applications.

Leaf Morphology: Altered Leaf Size.

Large leaves, such as those produced in plants overexpressing G189,G1451, G2430 and their functional equivalogs, generally increase plantbiomass. This provides benefit for crops where the vegetative portion ofthe plant is the marketable portion.

Leaf Morphology: Light Green and Variegated Leaves.

Transcription factor genes such as G635, G1494, G2144 and theirequivalogs that provide an altered appearance may positively affect aplant's value to the ornamental horticulture industry.

Leaf Morphology: Glossy Leaves.

Transcription factor genes such as G30, G1792, G2583 and theirequivalogs that induce the formation of glossy leaves generally do so byelevating levels of epidermal wax. Thus, the genes could be used toengineer changes in the composition and amount of leaf surfacecomponents, including waxes. The ability to manipulate wax composition,amount, or distribution could modify plant tolerance to drought and lowhumidity, or resistance to insects or pathogens. Additionally, wax maybe a valuable commodity in some species, and altering its accumulationand/or composition could enhance yield.

Seed Morphology: Altered Seed Coloration.

Presently disclosed transcription factor genes, including G156, G2105,G2085 have also been used to modify seed color, which, along with theequivalogs of these genes, could provide added appeal to seeds or seedproducts.

Seed Morphology: Altered Seed Size and Shape.

The introduction of presently disclosed transcription factor genes intoplants that increase (e.g., G450; G584; G1255; G2085; G2105; G2114) ordecrease (e.g., G1040). The size of seeds may have a significant impacton yield and appearance, particularly when the product is the seeditself (e.g., in the case of grains, legumes, nuts, etc.). Seed size, inaddition to seed coat integrity, thickness and permeability, seed watercontent and a number of other components including antioxidants andoligosaccharides, also affects affect seed longevity in storage, withlarger seeds often being more desirable for prolonged storage.

Transcription factor genes that alter seed shape, including G1040,G1062, G1255 and their equivalogs may have both ornamental applicationsand improve or broaden the appeal of seed products.

Leaf Biochemistry: Increased Leaf Wax.

Overexpression of transcription factors genes, including G975, G1792 andG2085 and their equivalogs, which results in increased leaf wax could beused to manipulate wax composition, amount, or distribution. Thesetranscription factors can improve yield in those plants and crops fromwhich wax is a valuable product. The genes may also be used to modifyplant tolerance to drought and/or low humidity or resistance to insects,as well as plant appearance (glossy leaves). The effect of increased waxdeposition on leaves of a plant like may improve water use efficiency.Manipulation of these genes may reduce the wax coating on sunflowerseeds; this wax fouls the oil extraction system during sunflower seedprocessing for oil. For the latter purpose or any other where waxreduction is valuable, antisense or cosuppression of the transcriptionfactor genes in a tissue-specific manner would be valuable.

Leaf Biochemistry: Leaf Prenyl Lipids, Including Tocopherol.

Prenyl lipids play a role in anchoring proteins in membranes ormembranous organelles. Thus modifying the prenyl lipid content of seedsand leaves could affect membrane integrity and function. One importantgroup of prenyl lipids, the tocopherols, have both anti-oxidant andvitamin E activity. A number of presently disclosed transcription factorgenes, including G214, G652, G748, G987, G1543, and G2509, have beenshown to modify the tocopherol composition of leaves in plants, andthese genes and their equivalogs may thus be used to alter prenyl lipidcontent of leaves.

Leaf Biochemistry: Increased Leaf Insoluble Sugars.

Overexpression of a number of presently disclosed transcription factors,including G211, resulted in plants with altered leaf insoluble sugarcontent. This transcription factor and its equivalogs that alter plantcell wall composition have several potential applications includingaltering food digestibility, plant tensile strength, wood quality,pathogen resistance and in pulp production. In particular, hemicelluloseis not desirable in paper pulps because of its lack of strength comparedwith cellulose. Thus modulating the amounts of cellulose vs.hemicellulose in the plant cell wall is desirable for the paper/lumberindustry. Increasing the insoluble carbohydrate content in variousfruits, vegetables, and other edible consumer products will result inenhanced fiber content. Increased fiber content would not only providehealth benefits in food products, but might also increase digestibilityof forage crops. In addition, the hemicellulose and pectin content offruits and berries affects the quality of jam and catsup made from them.Changes in hemicellulose and pectin content could result in a superiorconsumer product.

Leaf Biochemistry: Increased Leaf Anthocyanin.

Several presently disclosed transcription factor genes may be used toalter anthocyanin production in numerous plant species. Expression ofpresently disclosed transcription factor genes that increase flavonoidproduction in plants, including anthocyanins and condensed tannins, maybe used to alter in pigment production for horticultural purposes, andpossibly increasing stress resistance. G362, G663, G1482 and G1888 ortheir equivalogs, for example, could be used to alter anthocyaninproduction or accumulation. A number of flavonoids have been shown tohave antimicrobial activity and could be used to engineer pathogenresistance. Several flavonoid compounds have health promoting effectssuch as inhibition of tumor growth, prevention of bone loss andprevention of the oxidation of lipids. Increased levels of condensedtannins, in forage legumes would be an important agronomic trait becausethey prevent pasture bloat by collapsing protein foams within the rumen.For a review on the utilities of flavonoids and their derivatives, referto Dixon et al. (1999) Trends Plant Sci. 4: 394-400.

Leaf and Seed Biochemistry: Altered Fatty Acid Content.

A number of the presently disclosed transcription factor genes have beenshown to alter the fatty acid composition in plants, and seeds andleaves in particular. This modification suggests several utilities,including improving the nutritional value of seeds or whole plants.Dietary fatty acids ratios have been shown to have an effect on, forexample, bone integrity and remodeling (see, for example, Weiler (2000)Pediatr. Res. 47:5 692-697). The ratio of dietary fatty acids may alterthe precursor pools of long-chain polyunsaturated fatty acids that serveas precursors for prostaglandin synthesis. In mammalian connectivetissue, prostaglandins serve as important signals regulating the balancebetween resorption and formation in bone and cartilage. Thus dietaryfatty acid ratios altered in seeds may affect the etiology and outcomeof bone loss.

Transcription factors that reduce leaf fatty acids, for example, 16:3fatty acids, may be used to control thylakoid membrane development,including proplastid to chloroplast development. The genes that encodethese transcription factors might thus be useful for controlling thetransition from proplastid to chromoplast in fruits and vegetables. Itmay also be desirable to change the expression of these genes to preventcotyledon greening in Brassica napus or B. campestris to avoid green oildue to early frost.

A number of transcription factor genes are involved in mediating anaspect of the regulatory response to temperature. These genes may beused to alter the expression of desaturases that lead to production of18:3 and 16:3 fatty acids, the balance of which affects membranefluidity and mitigates damage to cell membranes and photosyntheticstructures at high and low temperatures.

Seed Biochemistry: Modified Seed Oil and Fatty Acid Content.

The composition of seeds, particularly with respect to seed oil amountsand/or composition, is very important for the nutritional and caloricvalue and production of various food and feed products. Several of thepresently disclosed transcription factor genes in seed lipid saturationthat alter seed oil content could be used to improve the heat stabilityof oils or to improve the nutritional quality of seed oil, by, forexample, reducing the number of calories in seed by decreasing oil orfatty acid content (e.g., G180; G192; G241; G1229; G1323; G1543),increasing the number of calories in animal feeds by increasing oil orfatty acid content (e.g. G162; G291; G427; G590; G598; G629, G715; G849;G1198, G1471; G1526; G1640; G1646, G1750; G1777; G1793; G1838; G1902;G1946; G1948; G2123; G2138; G2830), altering seed oil content (G504;G509; G519; G561; G567; G892; G961; G974; G1143; G1226; G1451; G1478;G1496; G1672; G1677; G1765; G2509; G2343), or altering the ratio ofsaturated to unsaturated lipids comprising the oils (e.g. G869; G1417;G2192).

Seed Biochemistry: Modified Seed Protein Content.

As with seed oils, the composition of seeds, particularly with respectto protein amounts and/or composition, is very important for thenutritional value and production of various food and feed products. Anumber of the presently disclosed transcription factor genes modify theprotein concentrations in seeds, including G162; G226; G1323; G1419;G1818, which increase seed protein, G427; G1777; G1903; G1946, whichdecrease seed protein, and G162; G241; G509; G567; G597; G849; G892;G988; G1478; G1634; G1637; G1652; G1677; G1820; G1958; G2509; G2117;G2509, which alter seed protein content, would provide nutritionalbenefits, and may be used to prolong storage, increase seed pest ordisease resistance, or modify germination rates.

Seed Biochemistry: Seed Prenyl Lipids.

Prenyl lipids play a role in anchoring proteins in membranes ormembranous organelles. Thus, modifying the prenyl lipid content of seedsand leaves could affect membrane integrity and function. A number ofpresently disclosed transcription factor genes have been shown to modifythe tocopherol composition of plants. α-Tocopherol is better known asvitamin E. Tocopherols such as α- and γ-tocopherol both haveanti-oxidant activity.

Seed Biochemistry: Seed Glucosinolates.

A number of glucosinolates have been shown to have anti-cancer activity;thus, increasing the levels or composition of these compounds byintroducing several of the presently disclosed transcription factors,including G484 and G2340, can have a beneficial effect on human diet.

Glucosinolates are undesirable components of the oilseeds used in animalfeed since they produce toxic effects. Low-glucosinolate varieties ofcanola, for example, have been developed to combat this problem.Glucosinolates form part of a plant's natural defense against insects.Modification of glucosinolate composition or quantity by introducingtranscription factors that affect these characteristics can thereforeafford increased protection from herbivores. Furthermore, in ediblecrops, tissue specific promoters can be used to ensure that thesecompounds accumulate specifically in tissues, such as the epidermis,which are not taken for consumption.

Seed Biochemistry: Increased Seed Anthocyanin.

Several presently disclosed transcription factor genes may be used toalter anthocyanin production in the seeds of plants. As with leafanthocyanins, expression of presently disclosed transcription factorgenes that increase flavonoid (anthocyanins and condensed tannins)production in seeds, including G663 and its equivalogs, may be used toalter in pigment production for horticultural purposes, and possiblyincreasing stress resistance, antimicrobial activity and healthpromoting effects such as inhibition of tumor growth, prevention of boneloss and prevention of the oxidation of lipids.

Leaf and Seed Biochemistry: Production of Seed and Leaf Phytosterols:

Presently disclosed transcription factor genes that modify levels ofphytosterols in plants may have at least two utilities. First,phytosterols are an important source of precursors for the manufactureof human steroid hormones. Thus, regulation of transcription factorexpression or activity could lead to elevated levels of important humansteroid precursors for steroid semi-synthesis. For example,transcription factors that cause elevated levels of campesterol inleaves, or sitosterols and stigmasterols in seed crops, would be usefulfor this purpose. Phytosterols and their hydrogenated derivativesphytostanols also have proven cholesterol-lowering properties, andtranscription factor genes that modify the expression of these compoundsin plants would thus provide health benefits.

Root Biochemistry: Increased Root Anthocyanin.

Presently disclosed transcription factor genes, including G663, may beused to alter anthocyanin production in the root of plants. As describedabove for seed anthocyanins, expression of presently disclosedtranscription factor genes that increase flavonoid (anthocyanins andcondensed tannins) production in seeds, including G663 and itsequivalogs, may be used to alter in pigment production for horticulturalpurposes, and possibly increasing stress resistance, antimicrobialactivity and health promoting effects such as inhibition of tumorgrowth, prevention of bone loss and prevention of the oxidation oflipids.

Light Response/Shade Avoidance:

altered cotyledon, hypocotyl, petiole development, altered leaforientation, constitutive photomorphogenesis, photomorphogenesis in lowlight. Presently disclosed transcription factor genes, including G183;G354; G1322; G1331; G1488; G1494; G1794; G2144; and G2555, that modify aplant's response to light may be useful for modifying plant growth ordevelopment, for example, photomorphogenesis in poor light, oraccelerating flowering time in response to various light intensities,quality or duration to which a non-transformed plant would not similarlyrespond. Examples of such responses that have been demonstrated includeleaf number and arrangement, and early flower bud appearancesElimination of shading responses may lead to increased plantingdensities with subsequent yield enhancement. As these genes may alsoalter plant architecture, they may find use in the ornamentalhorticulture industry.

Pigment: Increased Anthocyanin Level in Various Plant Organs andTissues.

In addition to seed, leaves and roots, as mentioned above, severalpresently disclosed transcription factor genes can be used to alteranthocyanin levels in one or more tissues. The potential utilities ofthese genes include alterations in pigment production for horticulturalpurposes, and possibly increasing stress resistance, antimicrobialactivity and health promoting effects such as inhibition of tumorgrowth, prevention of bone loss and prevention of the oxidation oflipids.

Miscellaneous Biochemistry: Diterpenes in Leaves and Other Plant Parts.

Depending on the plant species, varying amounts of diverse secondarybiochemicals (often lipophilic terpenes) are produced and exuded orvolatilized by trichomes. These exotic secondary biochemicals, which arerelatively easy to extract because they are on the surface of the leaf,have been widely used in such products as flavors and aromas, drugs,pesticides and cosmetics. Thus, the overexpression of genes that areused to produce diterpenes in plants may be accomplished by introducingtranscription factor genes that induce said overexpression. One class ofsecondary metabolites, the diterpenes, can effect several biologicalsystems such as tumor progression, prostaglandin synthesis and tissueinflammation. In addition, diterpenes can act as insect pheromones,termite allomones, and can exhibit neurotoxic, cytotoxic and antimitoticactivities. As a result of this functional diversity, diterpenes havebeen the target of research several pharmaceutical ventures. In mostcases where the metabolic pathways are impossible to engineer,increasing trichome density or size on leaves may be the only way toincrease plant productivity.

Miscellaneous Biochemistry: Production of Miscellaneous SecondaryMetabolites.

Microarray data suggests that flux through the aromatic amino acidbiosynthetic pathways and primary and secondary metabolite biosyntheticpathways are up-regulated. Presently disclosed transcription factorshave been shown to be involved in regulating alkaloid biosynthesis, inpart by up-regulating the enzymes indole-3-glycerol phosphatase andstrictosidine synthase. Phenylalanine ammonia lyase, chalcone synthaseand trans-cinnamate mono-oxygenase are also induced, and are involved inphenylpropenoid biosynthesis.

Antisense and Co-Suppression

In addition to expression of the nucleic acids of the invention as genereplacement or plant phenotype modification nucleic acids, the nucleicacids are also useful for sense and anti-sense suppression ofexpression, e.g., to down-regulate expression of a nucleic acid of theinvention, e.g., as a further mechanism for modulating plant phenotype.That is, the nucleic acids of the invention, or subsequences oranti-sense sequences thereof, can be used to block expression ofnaturally occurring homologous nucleic acids. A variety of sense andanti-sense technologies are known in the art, e.g., as set forth inLichtenstein and Nellen (1997) Antisense Technology: A PracticalApproach IRL Press at Oxford University Press, Oxford, U.K. Antisenseregulation is also described in Crowley et al. (1985) Cell 43: 633-641;Rosenberg et al. (1985) Nature 313: 703-706; Preiss et al. (1985) Nature313: 27-32; Melton (1985) Proc. Natl. Acad. Sci. 82: 144-148; Izant andWeintraub (1985) Science 229: 345-352; and Kim and Wold (1985) Cell 42:129-138. Additional methods for antisense regulation are known in theart. Antisense regulation has been used to reduce or inhibit expressionof plant genes in, for example in European Patent Publication No.271988. Antisense RNA may be used to reduce gene expression to produce avisible or biochemical phenotypic change in a plant (Smith et al. (1988)Nature, 334: 724-726; Smith et al. (1990) Plant Mol. Biol. 14: 369-379).In general, sense or anti-sense sequences are introduced into a cell,where they are optionally amplified, e.g., by transcription. Suchsequences include both simple oligonucleotide sequences and catalyticsequences such as ribozymes.

For example, a reduction or elimination of expression (i.e., a“knock-out”) of a transcription factor or transcription factor homologpolypeptide in a transgenic plant, e.g., to modify a plant trait, can beobtained by introducing an antisense construct corresponding to thepolypeptide of interest as a cDNA. For antisense suppression, thetranscription factor or homolog cDNA is arranged in reverse orientation(with respect to the coding sequence) relative to the promoter sequencein the expression vector. The introduced sequence need not be the fulllength cDNA or gene, and need not be identical to the cDNA or gene foundin the plant type to be transformed. Typically, the antisense sequenceneed only be capable of hybridizing to the target gene or RNA ofinterest. Thus, where the introduced sequence is of shorter length, ahigher degree of homology to the endogenous transcription factorsequence will be needed for effective antisense suppression. Whileantisense sequences of various lengths can be utilized, preferably, theintroduced antisense sequence in the vector will be at least 30nucleotides in length, and improved antisense suppression will typicallybe observed as the length of the antisense sequence increases.Preferably, the length of the antisense sequence in the vector will begreater than 100 nucleotides. Transcription of an antisense construct asdescribed results in the production of RNA molecules that are thereverse complement of mRNA molecules transcribed from the endogenoustranscription factor gene in the plant cell.

Suppression of endogenous transcription factor gene expression can alsobe achieved using a ribozyme. Ribozymes are RNA molecules that possesshighly specific endoribonuclease activity. The production and use ofribozymes are disclosed in U.S. Pat. No. 4,987,071 and U.S. Pat. No.5,543,508. Synthetic ribozyme sequences including antisense RNAs can beused to confer RNA cleaving activity on the antisense RNA, such thatendogenous mRNA molecules that hybridize to the antisense RNA arecleaved, which in turn leads to an enhanced antisense inhibition ofendogenous gene expression.

Vectors in which RNA encoded by a transcription factor or transcriptionfactor homolog cDNA is over-expressed can also be used to obtainco-suppression of a corresponding endogenous gene, e.g., in the mannerdescribed in U.S. Pat. No. 5,231,020 to Jorgensen. Such co-suppression(also termed sense suppression) does not require that the entiretranscription factor cDNA be introduced into the plant cells, nor doesit require that the introduced sequence be exactly identical to theendogenous transcription factor gene of interest. However, as withantisense suppression, the suppressive efficiency will be enhanced asspecificity of hybridization is increased, e.g., as the introducedsequence is lengthened, and/or as the sequence similarity between theintroduced sequence and the endogenous transcription factor gene isincreased.

Vectors expressing an untranslatable form of the transcription factormRNA, e.g., sequences comprising one or more stop codon, or nonsensemutation) can also be used to suppress expression of an endogenoustranscription factor, thereby reducing or eliminating its activity andmodifying one or more traits. Methods for producing such constructs aredescribed in U.S. Pat. No. 5,583,021. Preferably, such constructs aremade by introducing a premature stop codon into the transcription factorgene. Alternatively, a plant trait can be modified by gene silencingusing double-strand RNA (Sharp (1999) Genes and Development 13:139-141). Another method for abolishing the expression of a gene is byinsertion mutagenesis using the T-DNA of Agrobacterium tumefaciens.After generating the insertion mutants, the mutants can be screened toidentify those containing the insertion in a transcription factor ortranscription factor homolog gene. Plants containing a single transgeneinsertion event at the desired gene can be crossed to generatehomozygous plants for the mutation. Such methods are well known to thoseof skill in the art (See for example Koncz et al. (1992) Methods inArabidopsis Research, World Scientific Publishing Co. Pte. Ltd., RiverEdge, N.J.).

Alternatively, a plant phenotype can be altered by eliminating anendogenous gene, such as a transcription factor or transcription factorhomolog, e.g., by homologous recombination (Kempin et al. (1997) Nature389: 802-803).

A plant trait can also be modified by using the Cre-lox system (forexample, as described in U.S. Pat. No. 5,658,772). A plant genome can bemodified to include first and second lox sites that are then contactedwith a Cre recombinase. If the lox sites are in the same orientation,the intervening DNA sequence between the two sites is excised. If thelox sites are in the opposite orientation, the intervening sequence isinverted.

The polynucleotides and polypeptides of this invention can also beexpressed in a plant in the absence of an expression cassette bymanipulating the activity or expression level of the endogenous gene byother means, such as, for example, by ectopically expressing a gene byT-DNA activation tagging (Ichikawa et al. (1997) Nature 390 698-701;Kakimoto et al. (1996) Science 274: 982-985). This method entailstransforming a plant with a gene tag containing multiple transcriptionalenhancers and once the tag has inserted into the genome, expression of aflanking gene coding sequence becomes deregulated. In another example,the transcriptional machinery in a plant can be modified so as toincrease transcription levels of a polynucleotide of the invention (See,e.g., PCT Publications WO 96/06166 and WO 98/53057 which describe themodification of the DNA-binding specificity of zinc finger proteins bychanging particular amino acids in the DNA-binding motif).

The transgenic plant can also include the machinery necessary forexpressing or altering the activity of a polypeptide encoded by anendogenous gene, for example, by altering the phosphorylation state ofthe polypeptide to maintain it in an activated state.

Transgenic plants (or plant cells, or plant explants, or plant tissues)incorporating the polynucleotides of the invention and/or expressing thepolypeptides of the invention can be produced by a variety of wellestablished techniques as described above. Following construction of avector, most typically an expression cassette, including apolynucleotide, e.g., encoding a transcription factor or transcriptionfactor homolog, of the invention, standard techniques can be used tointroduce the polynucleotide into a plant, a plant cell, a plant explantor a plant tissue of interest. Optionally, the plant cell, explant ortissue can be regenerated to produce a transgenic plant.

The plant can be any higher plant, including gymnosperms,monocotyledonous and dicotyledenous plants. Suitable protocols areavailable for Leguminosae (alfalfa, soybean, clover, etc.), Umbelliferae(carrot, celery, parsnip), Cruciferae (cabbage, radish, rapeseed,broccoli, etc.), Curcurbitaceae (melons and cucumber), Gramineae (wheat,corn, rice, barley, millet, etc.), Solanaceae (potato, tomato, tobacco,peppers, etc.), and various other crops. See protocols described inAmmirato et al., Eds., (1984) Handbook of Plant Cell Culture—CropSpecies, Macmillan Publ. Co., New York, N.Y.; Shimamoto et al. (1989)Nature 338: 274-276; Fromm et al. (1990) Bio/Technol. 8: 833-839; andVasil et al. (1990) Bio/Technol. 8: 429-434.

Transformation and regeneration of both monocotyledonous anddicotyledonous plant cells is now routine, and the selection of the mostappropriate transformation technique will be determined by thepractitioner. The choice of method will vary with the type of plant tobe transformed; those skilled in the art will recognize the suitabilityof particular methods for given plant types. Suitable methods caninclude, but are not limited to: electroporation of plant protoplasts;liposome-mediated transformation; polyethylene glycol (PEG) mediatedtransformation; transformation using viruses; micro-injection of plantcells; micro-projectile bombardment of plant cells; vacuum infiltration;and Agrobacterium tumefaciens mediated transformation. Transformationmeans introducing a nucleotide sequence into a plant in a manner tocause stable or transient expression of the sequence.

Successful examples of the modification of plant characteristics bytransformation with cloned sequences which serve to illustrate thecurrent knowledge in this field of technology, and which are hereinincorporated by reference, include: U.S. Pat. Nos. 5,571,706; 5,677,175;5,510,471; 5,750,386; 5,597,945; 5,589,615; 5,750,871; 5,268,526;5,780,708; 5,538,880; 5,773,269; 5,736,369 and 5,610,042.

Following transformation, plants are preferably selected using adominant selectable marker incorporated into the transformation vector.Typically, such a marker will confer antibiotic or herbicide resistanceon the transformed plants, and selection of transformants can beaccomplished by exposing the plants to appropriate concentrations of theantibiotic or herbicide.

After transformed plants are selected and grown to maturity, thoseplants showing a modified trait are identified. The modified trait canbe any of those traits described above. Additionally, to confirm thatthe modified trait is due to changes in expression levels or activity ofthe polypeptide or polynucleotide of the invention can be determined byanalyzing mRNA expression using Northern blots, RT-PCR or microarrays,or protein expression using immunoblots or Western blots or gel shiftassays.

Integrated Systems—Sequence Identity

Additionally, the present invention may be an integrated system,computer or computer readable medium that comprises an instruction setfor determining the identity of one or more sequences in a database. Inaddition, the instruction set can be used to generate or identifysequences that meet any specified criteria. Furthermore, the instructionset may be used to associate or link certain functional benefits, suchimproved characteristics, with one or more identified sequence.

For example, the instruction set can include, e.g., a sequencecomparison or other alignment program, e.g., an available program suchas, for example, the Wisconsin Package Version 10.0, such as BLAST,FASTA, PILEUP, FINDPATTERNS or the like (GCG, Madison, Wis.). Publicsequence databases such as GenBank, EMBL, Swiss-Prot and PIR or privatesequence databases such as PHYTOSEQ sequence database (Incyte Genomics,Palo Alto, Calif.) can be searched.

Alignment of sequences for comparison can be conducted by the localhomology algorithm of Smith and Waterman (1981) Adv. Appl. Math. 2:482-489, by the homology alignment algorithm of Needleman and Wunsch(1970) J. Mol. Biol. 48: 443-453, by the search for similarity method ofPearson and Lipman (1988) Proc. Natl. Acad. Sci. 85: 2444-2448, bycomputerized implementations of these algorithms. After alignment,sequence comparisons between two (or more) polynucleotides orpolypeptides are typically performed by comparing sequences of the twosequences over a comparison window to identify and compare local regionsof sequence similarity. The comparison window can be a segment of atleast about 20 contiguous positions, usually about 50 to about 200, moreusually about 100 to about 150 contiguous positions. A description ofthe method is provided in Ausubel et al. supra.

A variety of methods for determining sequence relationships can be used,including manual alignment and computer assisted sequence alignment andanalysis. This later approach is a preferred approach in the presentinvention, due to the increased throughput afforded by computer assistedmethods. As noted above, a variety of computer programs for performingsequence alignment are available, or can be produced by one of skill.

One example algorithm that is suitable for determining percent sequenceidentity and sequence similarity is the BLAST algorithm, which isdescribed in Altschul et al. (1990) J. Mol. Biol. 215: 403-410. Softwarefor performing BLAST analyses is publicly available, e.g., through theNational Library of Medicine's National Center for BiotechnologyInformation (ncbi.nlm.nih; see at world wide web (www) NationalInstitutes of Health US government (gov) website). This algorithminvolves first identifying high scoring sequence pairs (HSPs) byidentifying short words of length W in the query sequence, which eithermatch or satisfy some positive-valued threshold score T when alignedwith a word of the same length in a database sequence. T is referred toas the neighborhood word score threshold (Altschul et al. supra). Theseinitial neighborhood word hits act as seeds for initiating searches tofind longer HSPs containing them. The word hits are then extended inboth directions along each sequence for as far as the cumulativealignment score can be increased. Cumulative scores are calculatedusing, for nucleotide sequences, the parameters M (reward score for apair of matching residues; always >0) and N (penalty score formismatching residues; always <0). For amino acid sequences, a scoringmatrix is used to calculate the cumulative score. Extension of the wordhits in each direction are halted when: the cumulative alignment scorefalls off by the quantity X from its maximum achieved value; thecumulative score goes to zero or below, due to the accumulation of oneor more negative-scoring residue alignments; or the end of eithersequence is reached. The BLAST algorithm parameters W, T, and Xdetermine the sensitivity and speed of the alignment. The BLASTN program(for nucleotide sequences) uses as defaults a wordlength (W) of 11, anexpectation (E) of 10, a cutoff of 100, M=5, N=−4, and a comparison ofboth strands. For amino acid sequences, the BLASTP program uses asdefaults a wordlength (W) of 3, an expectation (E) of 10, and theBLOSUM62 scoring matrix (see Henikoff and Henikoff (1992) Proc. Natl.Acad. Sci. 89: 10915-10919). Unless otherwise indicated, “sequenceidentity” here refers to the % sequence identity generated from atblastx using the NCBI version of the algorithm at the default settingsusing gapped alignments with the filter “off” (see, for example, NIH NLMNCBI website at ncbi.nlm.nih, supra).

In addition to calculating percent sequence identity, the BLASTalgorithm also performs a statistical analysis of the similarity betweentwo sequences (see, e.g. Karlin and Altschul (1993) Proc. Natl. Acad.Sci. 90: 5873-5787). One measure of similarity provided by the BLASTalgorithm is the smallest sum probability (P(N)), which provides anindication of the probability by which a match between two nucleotide oramino acid sequences would occur by chance. For example, a nucleic acidis considered similar to a reference sequence (and, therefore, in thiscontext, homologous) if the smallest sum probability in a comparison ofthe test nucleic acid to the reference nucleic acid is less than about0.1, or less than about 0.01, and or even less than about 0.001. Anadditional example of a useful sequence alignment algorithm is PILEUP.PILEUP creates a multiple sequence alignment from a group of relatedsequences using progressive, pairwise alignments. The program can align,e.g., up to 300 sequences of a maximum length of 5,000 letters.

The integrated system, or computer typically includes a user inputinterface allowing a user to selectively view one or more sequencerecords corresponding to the one or more character strings, as well asan instruction set which aligns the one or more character strings witheach other or with an additional character string to identify one ormore region of sequence similarity. The system may include a link of oneor more character strings with a particular phenotype or gene function.Typically, the system includes a user readable output element thatdisplays an alignment produced by the alignment instruction set.

The methods of this invention can be implemented in a localized ordistributed computing environment. In a distributed environment, themethods may implemented on a single computer comprising multipleprocessors or on a multiplicity of computers. The computers can belinked, e.g. through a common bus, but more preferably the computer(s)are nodes on a network. The network can be a generalized or a dedicatedlocal or wide-area network and, in certain preferred embodiments, thecomputers may be components of an intra-net or an internet.

Thus, the invention provides methods for identifying a sequence similaror homologous to one or more polynucleotides as noted herein, or one ormore target polypeptides encoded by the polynucleotides, or otherwisenoted herein and may include linking or associating a given plantphenotype or gene function with a sequence. In the methods, a sequencedatabase is provided (locally or across an inter or intra net) and aquery is made against the sequence database using the relevant sequencesherein and associated plant phenotypes or gene functions.

Any sequence herein can be entered into the database, before or afterquerying the database. This provides for both expansion of the databaseand, if done before the querying step, for insertion of controlsequences into the database. The control sequences can be detected bythe query to ensure the general integrity of both the database and thequery. As noted, the query can be performed using a web browser basedinterface. For example, the database can be a centralized publicdatabase such as those noted herein, and the querying can be done from aremote terminal or computer across an internet or intranet.

Any sequence herein can be used to identify a similar, homologous,paralogous, or orthologous sequence in another plant. This providesmeans for identifying endogenous sequences in other plants that may beuseful to alter a trait of progeny plants, which results from crossingtwo plants of different strain. For example, sequences that encode anortholog of any of the sequences herein that naturally occur in a plantwith a desired trait can be identified using the sequences disclosedherein. The plant is then crossed with a second plant of the samespecies but which does not have the desired trait to produce progenywhich can then be used in further crossing experiments to produce thedesired trait in the second plant. Therefore the resulting progeny plantcontains no transgenes; expression of the endogenous sequence may alsobe regulated by treatment with a particular chemical or other means,such as EMR. Some examples of such compounds well known in the artinclude: ethylene; cytokinins; phenolic compounds, which stimulate thetranscription of the genes needed for infection; specificmonosaccharides and acidic environments which potentiate vir geneinduction; acidic polysaccharides which induce one or more chromosomalgenes; and opines; other mechanisms include light or dark treatment (fora review of examples of such treatments, see, Winans (1992) Microbiol.Rev. 56: 12-31; Eyal et al. (1992) Plant Mol. Biol. 19: 589-599;Chrispeels et al. (2000) Plant Mol. Biol. 42: 279-290; Piazza et al.(2002) Plant Physiol. 128: 1077-1086).

Table 7 lists sequences discovered to be orthologous to a number ofrepresentative transcription factors of the present invention. Thecolumn headings include the transcription factors listed by SEQ ID NO;corresponding Gene ID (GID) numbers; the species from which theorthologs to the transcription factors are derived; the type of sequence(i.e., DNA or protein) discovered to be orthologous to the transcriptionfactors; and the SEQ ID NO of the orthologs, the latter corresponding tothe ortholog SEQ ID NOs listed in the Sequence Listing.

TABLE 7 Orthologs of Representative Arabidopsis Transcription FactorGenes SEQ ID NO: of Nucleotide SEQ ID NO: GID NO of Encoding of OrthologSequence type Orthologous Orthologous or Nucleotide used for ArabidopsisArabidopsis Encoding Ortholog Species from Which determinationTranscription Transcription Ortholog GID NO Ortholog is Derived (DNA orProtein) Factor Factor 459 Glycine max DNA G8 1 460 Glycine max DNA G8 1461 Glycine max DNA G8 1 462 Glycine max DNA G8 1 463 Oryza sativa DNAG8 1 464 Zea mays DNA G8 1 465 Zea mays DNA G8 1 466 Zea mays DNA G8 1467 Oryza sativa PRT G8 1 468 Glycine max DNA G19 3 469 Glycine max DNAG19 3 470 Glycine max DNA G19 3 471 Glycine max DNA G19 3 472 Oryzasativa DNA G19 3 473 Oryza sativa DNA G19 3 474 Oryza sativa DNA G19 3475 Zea mays DNA G19 3 476 Zea mays DNA G19 3 477 Glycine max DNA G22 5478 Glycine max DNA G22 5 479 Glycine max DNA G24 7 480 Glycine max DNAG24 7 481 Glycine max DNA G24 7 482 Glycine max DNA G24 7 483 Glycinemax DNA G24 7 484 Glycine max DNA G24 7 485 Glycine max DNA G24 7 486Oryza sativa DNA G24 7 487 Zea mays DNA G24 7 488 Oryza sativa PRT G24 7489 Oryza sativa PRT G24 7 490 Oryza sativa PRT G24 7 491 Glycine maxDNA G28 9 492 Glycine max DNA G28 9 493 Glycine max DNA G28 9 494Glycine max DNA G28 9 495 Glycine max DNA G28 9 496 Glycine max DNA G289 497 Glycine max DNA G28 9 498 Glycine max DNA G28 9 499 Oryza sativaDNA G28 9 500 Zea mays DNA G28 9 501 Oryza sativa PRT G28 9 502 Oryzasativa PRT G28 9 503 Mesembryanthemum PRT G28 9 crystallinum 504 Glycinemax DNA G47, G2133  11, 407 505 Oryza sativa PRT G47, G2133  11, 407 506Glycine max DNA G157, G859, 15, 165, 349, G1842, G1843 351 507 Glycinemax DNA G175, G877  19, 173 508 Oryza sativa DNA G175, G877  19, 173 509Zea mays DNA G175, G877  19, 173 510 Zea mays DNA G175, G877  19, 173511 Zea mays DNA G175, G877  19, 173 512 Oryza sativa PRT G175, G877 19, 173 513 Oryza sativa PRT G175, G877  19, 173 514 Oryza sativa PRTG175, G877  19, 173 515 Nicotiana tabacum PRT G175, G877  19, 173 516Glycine max DNA G180 21 517 Glycine max DNA G180 21 518 Oryza sativa DNAG180 21 519 Zea mays DNA G180 21 520 Solanum tuberosum DNA G180 21 521Oryza sativa PRT G180 21 522 Capsella rubella PRT G183 23 523 Glycinemax DNA G188 25 524 Zea mays DNA G188 25 525 Oryza sativa PRT G188 25526 Oryza sativa PRT G188 25 527 Glycine max DNA G189 27 528 Nicotianatabacum PRT G189 27 529 Glycine max DNA G192 29 530 Oryza sativa PRTG192 29 531 Glycine max DNA G196 31 532 Zea mays DNA G196 31 533 Zeamays DNA G196 31 534 Oryza sativa PRT G196 31 535 Oryza sativa PRT G19631 536 Oryza sativa PRT G196 31 537 Oryza sativa PRT G196 31 538 Glycinemax DNA G211 33 539 Oryza sativa DNA G211 33 540 Oryza sativa PRT G21133 541 Glycine max DNA G214, G680  35, 145 542 Glycine max DNA G214,G680  35, 145 543 Glycine max DNA G214, G680  35, 145 544 Glycine maxDNA G214, G680  35, 145 545 Oryza sativa DNA G214, G680  35, 145 546Oryza sativa DNA G214, G680  35, 145 547 Zea mays DNA G214, G680  35,145 548 Zea mays DNA G214, G680  35, 145 549 Zea mays DNA G214, G680 35, 145 550 Glycine max DNA G226, G682  37, 147 551 Glycine max DNAG226 37 552 Glycine max DNA G226, G682  37, 147 553 Glycine max DNAG226, G682  37, 147 554 Glycine max DNA G226, G682  37, 147 555 Oryzasativa DNA G226, G682  37, 147 556 Zea mays DNA G226, G682  37, 147 557Zea mays DNA G226, G682  37, 147 558 Oryza sativa PRT G226, G682  37,147 559 Oryza sativa PRT G226, G682  37, 147 560 Glycine max DNA G241 39561 Glycine max DNA G241 39 562 Glycine max DNA G241 39 563 Oryza sativaDNA G241 39 564 Zea mays DNA G241 39 565 Zea mays DNA G241 39 566 Zeamays DNA G241 39 567 Zea mays DNA G241 39 568 Zea mays DNA G241 39 569Nicotiana tabacum PRT G241 39 570 Glycine max DNA G254 43 571 Glycinemax DNA G256 45 572 Glycine max DNA G256 45 573 Glycine max DNA G256 45574 Glycine max DNA G256 45 575 Glycine max DNA G256 45 576 Glycine maxDNA G256 45 577 Glycine max DNA G256 45 578 Oryza sativa DNA G256 45 579Zea mays DNA G256 45 580 Zea mays DNA G256 45 581 Zea mays DNA G256 45582 Zea mays DNA G256 45 583 Zea mays DNA G256 45 584 Zea mays DNA G25645 585 G3500 Lycopersicon DNA G256 45 esculentum 586 G3501 LycopersiconDNA G256 45 esculentum 587 G3385 Oryza sativa PRT G256 45 588 G3386Oryza sativa PRT G256 45 589 Oryza sativa PRT G256 45 590 G3384 Oryzasativa PRT G256 45 591 Oryza sativa PRT G256 45 592 G3502 Oryza sativajaponica PRT G256 45 593 G3500 Lycopersicon PRT G256 45 esculentum 594G3501 Lycopersicon PRT G256 45 esculentum 595 Oryza sativa DNA G278 47596 Zea mays DNA G278 47 597 Oryza sativa PRT G278 47 598 Glycine maxDNA G312 53 599 Zea mays DNA G312 53 600 Euphorbia esula DNA G312 53 601Glycine max DNA G325 55 602 Glycine max DNA G343 57 603 Glycine max DNAG343 57 604 Glycine max DNA G343 57 605 Oryza sativa DNA G343 57 606Oryza sativa DNA G343 57 607 Oryza sativa PRT G343 57 608 Oryza sativaPRT G343 57 609 Oryza sativa PRT G343 57 610 Glycine max DNA G353, G35459, 61 611 Glycine max DNA G353, G354 59, 61 612 Glycine max DNA G353,G354 59, 61 613 Oryza sativa DNA G353, G354 59, 61 614 Zea mays DNAG353, G354 59, 61 615 Zea mays DNA G353, G354 59, 61 616 Zea mays DNAG353, G354 59, 61 617 Zea mays DNA G353, G354 59, 61 618 Zea mays DNAG353, G354 59, 61 619 Zea mays DNA G353, G354 59, 61 620 Zea mays DNAG353, G354 59, 61 621 Oryza sativa PRT G353, G354 59, 61 622 Oryzasativa PRT G353, G354 59, 61 623 Oryza sativa PRT G353, G354 59, 61 624Oryza sativa PRT G353, G354 59, 61 625 Oryza sativa PRT G353, G354 59,61 626 Oryza sativa PRT G353, G354 59, 61 627 Glycine max DNA G361, G36263, 65 628 Glycine max DNA G361, G362 63, 65 629 Glycine max DNA G361 63630 Glycine max DNA G361, G362 63, 65 631 Glycine max DNA G361, G362 63,65 632 Oryza sativa DNA G361, G362 63, 65 633 Zea mays DNA G361, G36263, 65 634 Zea mays DNA G361, G362 63, 65 635 Oryza sativa PRT G361,G362 63, 65 636 Oryza sativa PRT G361, G362 63, 65 637 Oryza sativa PRTG361, G362 63, 65 638 Oryza sativa PRT G361, G362 63, 65 639 Oryzasativa PRT G361, G362 63, 65 640 Glycine max DNA G390, G391, 69, 71, 77G438 641 Glycine max DNA G390, G391, 69, 71, 77 G438 642 Glycine max DNAG390, G391, 69, 71, 77 G438 643 Glycine max DNA G390, G391, 69, 71, 77G438 644 Glycine max DNA G390, G391, 69, 71, 77 G438 645 Glycine max DNAG390, G391, 69, 71, 77 G438 646 Glycine max DNA G390, G391, 69, 71, 77G438 647 Glycine max DNA G390, G391 69, 71 648 Glycine max DNA G390,G391, 69, 71, 77 G438 649 Glycine max DNA G390, G391, 69, 71, 77 G438650 Oryza sativa DNA G390 69 651 Oryza sativa DNA G390, G438 69, 77 652Zea mays DNA G390, G391, 69, 71, 77 G438 653 Zea mays DNA G390, G391,69, 71, 77 G438 654 Zea mays DNA G390, G391, 69, 71, 77 G438 655 Zeamays DNA G390, G391 69, 71 656 Zea mays DNA G390, G391, 69, 71, 77 G438657 Zea mays DNA G390, G391, 69, 71, 77 G438 658 Zea mays DNA G390,G391, 69, 71, 77 G438 659 Zea mays DNA G390, G391, 69, 71, 77 G438 660Zea mays DNA G390, G391, 69, 71, 77 G438 661 Zea mays DNA G390, G391,69, 71, 77 G438 662 Zea mays DNA G390, G391, 69, 71, 77 G438 663Lycopersicon DNA G390, G391, 69, 71, 77 esculentum G438 664 Oryza sativaDNA G391, G438 71, 77 665 Oryza sativa PRT G390, G391, 69, 71, 77 G438666 Oryza sativa PRT G390, G391, 69, 71, 77 G438 667 Oryza sativa PRTG390, G391, 69, 71, 77 G438 668 Oryza sativa PRT G390, G391, 69, 71, 77G438 669 Physcomitrella PRT G391 71 patens 670 Glycine max DNA G409 73671 Glycine max DNA G409 73 672 Glycine max DNA G409 73 673 Glycine maxDNA G409 73 674 Glycine max DNA G409 73 675 Glycine max DNA G409 73 676Glycine max DNA G409 73 677 Glycine max DNA G409 73 678 Oryza sativa DNAG409 73 679 Oryza sativa DNA G409 73 680 Oryza sativa DNA G409 73 681Zea mays DNA G409 73 682 Zea mays DNA G409 73 683 Zea mays DNA G409 73684 Zea mays DNA G409 73 685 Zea mays DNA G409 73 686 Zea mays DNA G40973 687 Zea mays DNA G409 73 688 Glycine max DNA G427 75 689 Glycine maxDNA G427 75 690 Glycine max DNA G427 75 691 Glycine max DNA G427 75 692Glycine max DNA G427 75 693 Glycine max DNA G427 75 694 Glycine max DNAG427 75 695 Glycine max DNA G427 75 696 Glycine max DNA G427 75 697Glycine max DNA G427 75 698 Oryza sativa DNA G427 75 699 Zea mays DNAG427 75 700 Zea mays DNA G427 75 701 Zea mays DNA G427 75 702 Zea maysDNA G427 75 703 Zea mays DNA G427 75 704 Zea mays DNA G427 75 705 Zeamays DNA G427 75 706 Zea mays DNA G427 75 707 Zea mays DNA G427 75 708Oryza sativa PRT G427 75 709 Oryza sativa PRT G427 75 710 Oryza sativaPRT G427 75 711 Malus x domestica PRT G427 75 712 Nicotiana tabacum PRTG427 75 713 Lycopersicon PRT G427 75 esculentum 714 Glycine max DNA G43877 715 Oryza sativa DNA G438 77 716 Oryza sativa DNA G438 77 717 Oryzasativa DNA G438 77 718 Oryza sativa DNA G438 77 719 Zea mays DNA G438 77720 Physcomitrella PRT G438 77 patens 721 Oryza sativa PRT G438 77 722Glycine max DNA G450 79 723 Glycine max DNA G450 79 724 Glycine max DNAG450 79 725 Glycine max DNA G450 79 726 Glycine max DNA G450 79 727Glycine max DNA G450 79 728 Glycine max DNA G450 79 729 Glycine max DNAG450 79 730 Glycine max DNA G450 79 731 Oryza sativa DNA G450 79 732Oryza sativa DNA G450 79 733 Zea mays DNA G450 79 734 Zea mays DNA G45079 735 Zea mays DNA G450 79 736 Oryza sativa PRT G450 79 737 Oryzasativa PRT G450 79 738 Oryza sativa PRT G450 79 739 Oryza sativa PRTG450 79 740 Oryza sativa DNA G464 81 741 Zea mays DNA G464 81 742 Oryzasativa PRT G464 81 743 Glycine max DNA G470 83 744 Oryza sativa DNA G47083 745 Oryza sativa DNA G470 83 746 Glycine max DNA G481, G482 87, 89747 Glycine max DNA G481, G482 87, 89 748 Glycine max DNA G481, G482 87,89 749 Glycine max DNA G481, G482 87, 89 750 Glycine max DNA G481, G48287, 89 751 Glycine max DNA G481, G482 87, 89 752 Glycine max DNA G481,G482 87, 89 753 Glycine max DNA G481, G482 87, 89 754 Glycine max DNAG481 87 755 Glycine max DNA G481 87 756 Oryza sativa DNA G481 87 757Oryza sativa DNA G481, G482 87, 89 758 Zea mays DNA G481 87 759 Zea maysDNA G481, G482 87, 89 760 Zea mays DNA G481, G482 87, 89 761 Zea maysDNA G481, G482 87, 89 762 Zea mays DNA G481, G482 87, 89 763 Zea maysDNA G481, G482 87, 89 764 Zea mays DNA G481, G482 87, 89 765 Zea maysDNA G481, G482 87, 89 766 Zea mays DNA G481, G482 87, 89 767 Zea maysDNA G481, G482 87, 89 768 Gossypium arboreum DNA G481, G482 87, 89 769Glycine max DNA G481, G482 87, 89 770 Gossypium hirsutum DNA G481, G48287, 89 771 Lycopersicon DNA G481, G482 87, 89 esculentum 772Lycopersicon DNA G481, G482 87, 89 esculentum 773 Medicago truncatulaDNA G481, G482 87, 89 774 Lycopersicon DNA G481, G482 87, 89 esculentum775 Solanum tuberosum DNA G481, G482 87, 89 776 Triticum aestivum DNAG481, G482 87, 89 777 Hordeum vulgare DNA G481, G482 87, 89 778 TriticumDNA G481, G482 87, 89 monococcum 779 Glycine max DNA G482 89 780 Oryzasativa PRT G481, G482 87, 89 781 Oryza sativa PRT G481, G482 87, 89 782Oryza sativa PRT G481, G482 87, 89 783 Oryza sativa PRT G481, G482 87,89 784 Oryza sativa PRT G481, G482 87, 89 785 Zea mays PRT G481, G48287, 89 786 Zea mays PRT G481, G482 87, 89 787 Oryza sativa PRT G481,G482 87, 89 788 Oryza sativa PRT G481, G482 87, 89 789 Oryza sativa PRTG481, G482 87, 89 790 Oryza sativa PRT G481, G482 87, 89 791 Oryzasativa PRT G481, G482 87, 89 792 Oryza sativa PRT G481, G482 87, 89 793Oryza sativa PRT G481, G482 87, 89 794 Oryza sativa PRT G481, G482 87,89 795 Oryza sativa PRT G481, G482 87, 89 796 Oryza sativa PRT G481,G482 87, 89 797 Glycine max PRT G481, G482 87, 89 798 Glycine max PRTG481, G482 87, 89 799 Glycine max PRT G481, G482 87, 89 800 Glycine maxPRT G481, G482 87, 89 801 Glycine max PRT G481, G482 87, 89 802 Glycinemax PRT G481, G482 87, 89 803 Glycine max PRT G481, G482 87, 89 804 Zeamays PRT G481, G482 87, 89 805 Zea mays PRT G481, G482 87, 89 806 Zeamays PRT G481, G482 87, 89 807 Zea mays PRT G481, G482 87, 89 808Glycine max DNA G484 91 809 Glycine max DNA G484 91 810 Glycine max DNAG484 91 811 Glycine max DNA G484 91 812 Glycine max DNA G484 91 813Glycine max DNA G484 91 814 Glycine max DNA G484 91 815 Glycine max DNAG484 91 816 Glycine max DNA G484 91 817 Glycine max DNA G484 91 818Oryza sativa DNA G484 91 819 Zea mays DNA G484 91 820 Zea mays DNA G48491 821 Zea mays DNA G484 91 822 Zea mays DNA G484 91 823 Zea mays DNAG484 91 824 Oryza sativa PRT G484 91 825 Glycine max DNA G489 93 826Glycine max DNA G489 93 827 Glycine max DNA G489 93 828 Glycine max DNAG489 93 829 Glycine max DNA G489 93 830 Glycine max DNA G489 93 831Glycine max DNA G489 93 832 Oryza sativa DNA G489 93 833 Oryza sativaDNA G489 93 834 Zea mays DNA G489 93 835 Oryza sativa PRT G489 93 836Oryza sativa PRT G489 93 837 Oryza sativa PRT G489 93 838 Glycine maxDNA G504 97 839 Glycine max DNA G504 97 840 Glycine max DNA G504 97 841Glycine max DNA G504 97 842 Glycine max DNA G504 97 843 Glycine max DNAG504 97 844 Glycine max DNA G504 97 845 Oryza sativa DNA G504 97 846Oryza sativa DNA G504 97 847 Zea mays DNA G504 97 848 Zea mays DNA G50497 849 Zea mays DNA G504 97 850 Zea mays DNA G504 97 851 Oryza sativaPRT G504 97 852 Oryza sativa PRT G504 97 853 Oryza sativa PRT G504 97854 Oryza sativa PRT G504 97 855 Lycopersicon DNA G509 99 esculentum 856Glycine max DNA G509 99 857 Glycine max DNA G509 99 858 Glycine max DNAG509 99 859 Oryza sativa DNA G509 99 860 Oryza sativa DNA G509 99 861Zea mays DNA G509 99 862 Zea mays DNA G509 99 863 Zea mays DNA G509 99864 Zea mays DNA G509 99 865 Oryza sativa PRT G509 99 866 Oryza sativaPRT G509 99 867 Oryza sativa PRT G509 99 868 Glycine max DNA G519 101869 Glycine max DNA G519 101 870 Glycine max DNA G519 101 871 Glycinemax DNA G519 101 872 Glycine max DNA G519 101 873 Glycine max DNA G519101 874 Glycine max DNA G519 101 875 Glycine max DNA G519 101 876Glycine max DNA G519 101 877 Oryza sativa DNA G519 101 878 Oryza sativaDNA G519 101 879 Oryza sativa DNA G519 101 880 Zea mays DNA G519 101 881Zea mays DNA G519 101 882 Zea mays DNA G519 101 883 Zea mays DNA G519101 884 Zea mays DNA G519 101 885 Zea mays DNA G519 101 886 Zea mays DNAG519 101 887 Zea mays DNA G519 101 888 Zea mays DNA G519 101 889 Zeamays DNA G519 101 890 Oryza sativa PRT G519 101 891 Oryza sativa PRTG519 101 892 Glycine max DNA G545 103 893 Glycine max DNA G545 103 894Glycine max DNA G545 103 895 Glycine max DNA G545 103 896 Glycine maxDNA G545 103 897 Glycine max DNA G545 103 898 Glycine max DNA G545 103899 Oryza sativa DNA G545 103 900 Zea mays DNA G545 103 901 Zea mays DNAG545 103 902 Zea mays DNA G545 103 903 Oryza sativa PRT G545 103 904Oryza sativa PRT G545 103 905 Oryza sativa PRT G545 103 906 Oryza sativaPRT G545 103 907 Datisca glomerata PRT G545 103 908 Oryza sativa DNAG546 105 909 Zea mays DNA G561 107 910 Sinapis alba PRT G561 107 911Raphanus sativus PRT G561 107 912 Brassica napus PRT G561 107 913Brassica napus PRT G561 107 914 Glycine max DNA G562 109 915 Glycine maxDNA G562 109 916 Glycine max DNA G562 109 917 Glycine max DNA G562 109918 Glycine max DNA G562 109 919 Zea mays DNA G562 109 920 Zea mays DNAG562 109 921 Zea mays DNA G562 109 922 Oryza sativa PRT G562 109 923Oryza sativa PRT G562 109 924 Glycine max DNA G567 111 925 Oryza sativaDNA G567 111 926 Oryza sativa PRT G567 111 927 Glycine max DNA G568 113928 Glycine max DNA G568 113 929 Oryza sativa DNA G568 113 930 Oryzasativa DNA G568 113 931 Oryza sativa DNA G568 113 932 Zea mays DNA G568113 933 Oryza sativa PRT G568 113 934 Populus balsamifera PRT G568 113subsp. trichocarpa x Populus deltoides 935 Glycine max DNA G584 115 936Glycine max DNA G584 115 937 Glycine max DNA G584 115 938 Glycine maxDNA G584 115 939 Glycine max DNA G584 115 940 Zea mays DNA G584 115 941Zea mays DNA G584 115 942 Zea mays DNA G584 115 943 Oryza sativa PRTG584 115 944 Glycine max DNA G585 117 945 Glycine max DNA G585 117 946Glycine max DNA G585 117 947 Glycine max DNA G585 117 948 Oryza sativaDNA G585 117 949 Zea mays DNA G585 117 950 Zea mays DNA G585 117 951 Zeamays DNA G585 117 952 Zea mays DNA G585 117 953 Oryza sativa PRT G585117 954 Oryza sativa PRT G585 117 955 Oryza sativa PRT G585 117 956Oryza sativa PRT G585 117 957 Oryza sativa PRT G585 117 958 Oryza sativaPRT G585 117 959 Gossypium hirsutum PRT G585 117 960 Antirrhinum majusPRT G585 117 961 Glycine max DNA G590 119 962 Glycine max DNA G590 119963 Glycine max DNA G590 119 964 Oryza sativa DNA G590 119 965 Zea maysDNA G590 119 966 Oryza sativa PRT G590 119 967 Oryza sativa PRT G590 119968 Oryza sativa DNA G597 123 969 Oryza sativa DNA G597 123 970 Oryzasativa DNA G597 123 971 Zea mays DNA G597 123 972 Zea mays DNA G597 123973 Zea mays DNA G597 123 974 Zea mays DNA G597 123 975 Zea mays DNAG597 123 976 Zea mays DNA G597 123 977 Zea mays DNA G597 123 978 Zeamays DNA G597 123 979 Zea mays DNA G597 123 980 Zea mays DNA G597 123981 Oryza sativa DNA G634 127 982 Oryza sativa DNA G634 127 983 Oryzasativa DNA G634 127 984 Zea mays DNA G634 127 985 Zea mays DNA G634 127986 Zea mays DNA G634 127 987 Oryza sativa PRT G634 127 988 Oryza sativaPRT G634 127 989 Glycine max DNA G635 129 990 Glycine max DNA G635 129991 Oryza sativa DNA G635 129 992 Oryza sativa DNA G635 129 993 Zea maysDNA G635 129 994 Oryza sativa PRT G635 129 995 Glycine max DNA G636 131996 Glycine max DNA G636 131 997 Glycine max DNA G636 131 998 Glycinemax DNA G636 131 999 Glycine max DNA G636 131 1000 Glycine max DNA G636131 1001 Glycine max DNA G636 131 1002 Glycine max DNA G636 131 1003Oryza sativa DNA G636 131 1004 Oryza sativa DNA G636 131 1005 Oryzasativa DNA G636 131 1006 Oryza sativa DNA G636 131 1007 Zea mays DNAG636 131 1008 Zea mays DNA G636 131 1009 Zea mays DNA G636 131 1010 Zeamays DNA G636 131 1011 Pisum sativum PRT G636 131 1012 Glycine max DNAG638 133 1013 Glycine max DNA G638 133 1014 Glycine max DNA G638 1331015 Glycine max DNA G638 133 1016 Medicago truncatula DNA G638 133 1017Glycine max DNA G652 135 1018 Glycine max DNA G652 135 1019 Glycine maxDNA G652 135 1020 Glycine max DNA G652 135 1021 Glycine max DNA G652 1351022 Glycine max DNA G652 135 1023 Glycine max DNA G652 135 1024 Glycinemax DNA G652 135 1025 Oryza sativa DNA G652 135 1026 Oryza sativa DNAG652 135 1027 Oryza sativa DNA G652 135 1028 Zea mays DNA G652 135 1029Zea mays DNA G652 135 1030 Zea mays DNA G652 135 1031 Zea mays DNA G652135 1032 Zea mays DNA G652 135 1033 Zea mays DNA G652 135 1034 Zea maysDNA G652 135 1035 Oryza sativa PRT G652 135 1036 Oryza sativa PRT G652135 1037 Oryza sativa PRT G652 135 1038 Oryza sativa PRT G652 135 1039Oryza sativa PRT G652 135 1040 Oryza sativa PRT G652 135 1041 Oryzasativa PRT G652 135 1042 Oryza sativa PRT G652 135 1043 Glycine max DNAG663 137 1044 Glycine max DNA G664 139 1045 Glycine max DNA G664 1391046 Glycine max DNA G664 139 1047 Glycine max DNA G664 139 1048 Glycinemax DNA G664 139 1049 Glycine max DNA G664 139 1050 Glycine max DNA G664139 1051 Oryza sativa DNA G664 139 1052 Oryza sativa DNA G664 139 1053Oryza sativa DNA G664 139 1054 Oryza sativa DNA G664 139 1055 Zea maysDNA G664 139 1056 Zea mays DNA G664 139 1057 Zea mays DNA G664 139 1058Zea mays DNA G664 139 1059 Zea mays DNA G664 139 1060 Zea mays DNA G664139 1061 Zea mays DNA G664 139 1062 Zea mays DNA G664 139 1063 G3509Lycopersicon DNA G664 139 esculentum 1064 G3506 Oryza sativa PRT G664139 1065 G3504 Oryza sativa PRT G664 139 1066 Oryza sativa PRT G664 1391067 Oryza sativa PRT G664 139 1068 G3503 Oryza sativa indica PRT G664139 1069 G3505 Oryza sativa japonica PRT G664 139 1070 G3507 Oryzasativa japonica PRT G664 139 1071 G3508 Oryza sativa japonica PRT G664139 1072 G3509 Lycopersicon PRT G664 139 esculentum 1073 Hordeum vulgarePRT G664 139 subsp. vulgare 1074 Oryza sativa DNA G680 145 1075 Zea maysDNA G680 145 1076 Glycine max DNA G682 147 1077 Hordeum vulgare DNA G682147 subsp. vulgare 1078 Populus tremula x DNA G682 147 Populustremuloides 1079 Triticum aestivum DNA G682 147 1080 Gossypium arboreumDNA G682 147 1081 Oryza sativa PRT G682 147 1082 Oryza sativa PRT G682147 1083 Glycine max PRT G682 147 1084 Glycine max PRT G682 147 1085Glycine max PRT G682 147 1086 Glycine max PRT G682 147 1087 Glycine maxPRT G682 147 1088 Glycine max PRT G682 147 1089 Zea mays PRT G682 1471090 Zea mays PRT G682 147 1091 Glycine max DNA G715, G1646 149, 3131092 Glycine max DNA G715, G1646 149, 313 1093 Glycine max DNA G715,G1646 149, 313 1094 Oryza sativa DNA G715, G1646 149, 313 1095 Oryzasativa DNA G715, G1646 149, 313 1096 Zea mays DNA G715, G1646 149, 3131097 Zea mays DNA G715, G1646 149, 313 1098 Zea mays DNA G715, G1646149, 313 1099 Zea mays DNA G715, G1646 149, 313 1100 Zea mays DNA G715,G1646 149, 313 1101 Zea mays DNA G715, G1646 149, 313 1102 Zea mays DNAG715, G1646 149, 313 1103 Zea mays DNA G715, G1646 149, 313 1104 Zeamays DNA G715, G1646 149, 313 1105 Oryza sativa PRT G715, G1646 149, 3131106 Oryza sativa PRT G715, G1646 149, 313 1107 Oryza sativa PRT G715,G1646 149, 313 1108 Oryza sativa PRT G715, G1646 149, 313 1109 Oryzasativa PRT G715, G1646 149, 313 1110 Oryza sativa PRT G715, G1646 149,313 1111 Glycine max DNA G720 151 1112 Glycine max DNA G720 151 1113Glycine max DNA G720 151 1114 Glycine max DNA G720 151 1115 Medicagotruncatula DNA G720 151 1116 Lycopersicon DNA G720 151 esculentum 1117Lycopersicon DNA G720 151 esculentum 1118 Lycopersicon DNA G720 151esculentum 1119 Solanum tuberosum DNA G720 151 1120 Glycine max DNA G736153 1121 Glycine max DNA G736 153 1122 Oryza sativa PRT G736 153 1123Glycine max DNA G748 155 1124 Glycine max DNA G748 155 1125 Glycine maxDNA G748 155 1126 Oryza sativa DNA G748 155 1127 Oryza sativa DNA G748155 1128 Zea mays DNA G748 155 1129 Oryza sativa PRT G748 155 1130 Oryzasativa PRT G748 155 1131 Oryza sativa PRT G748 155 1132 Oryza sativa PRTG748 155 1133 Cucurbita maxima PRT G748 155 1134 Glycine max DNA G789,G1494 159, 291 1135 Glycine max DNA G789, G1494 159, 291 1136 Oryzasativa DNA G789 159 1137 Oryza sativa DNA G789, G1494 159, 291 1138 Zeamays DNA G789, G1494 159, 291 1139 Oryza sativa PRT G789, G1494 159, 2911140 Oryza sativa PRT G789, G1494 159, 291 1141 Oryza sativa PRT G789,G1494 159, 291 1142 Glycine max DNA G801 161 1143 Glycine max DNA G801161 1144 Zea mays DNA G801 161 1145 Glycine max DNA G849 163 1146Glycine max DNA G849 163 1147 Glycine max DNA G849 163 1148 Glycine maxDNA G849 163 1149 Glycine max DNA G849 163 1150 Glycine max DNA G849 1631151 Zea mays DNA G849 163 1152 Zea mays DNA G849 163 1153 Zea mays DNAG849 163 1154 Glycine max DNA G864 167 1155 Glycine max DNA G864 1671156 Zea mays DNA G864 167 1157 Oryza sativa PRT G864 167 1158 Oryzasativa PRT G864 167 1159 Glycine max DNA G867, G1930 169, 369 1160Glycine max DNA G867, G1930 169, 369 1161 Glycine max DNA G867, G1930169, 369 1162 Glycine max DNA G867, G1930 169, 369 1163 Glycine max DNAG867, G1930 169, 369 1164 Glycine max DNA G867 169 1165 Oryza sativa DNAG867 169 1166 Oryza sativa DNA G867, G1930 169, 369 1167 Zea mays DNAG867, G1930 169, 369 1168 Zea mays DNA G867, G1930 169, 369 1169 Zeamays DNA G867, G1930 169, 369 1170 Zea mays DNA G867, G1930 169, 3691171 Glycine max DNA G867, G1930 169, 369 1172 Mesembryanthemum DNAG867, G1930 169, 369 crystallinum 1173 Lycopersicon DNA G867, G1930 169,369 esculentum 1174 Solanum tuberosum DNA G867, G1930 169, 369 1175Hordeum vulgare DNA G867, G1930 169, 369 1176 Oryza sativa PRT G867,G1930 169, 369 1177 Oryza sativa PRT G867, G1930 169, 369 1178 Oryzasativa PRT G867, G1930 169, 369 1179 Oryza sativa PRT G867, G1930 169,369 1180 Oryza sativa PRT G867, G1930 169, 369 1181 Oryza sativa PRTG867, G1930 169, 369 1182 Glycine max PRT G867, G1930 169, 369 1183Glycine max PRT G867, G1930 169, 369 1184 Glycine max PRT G867, G1930169, 369 1185 Zea mays PRT G867, G1930 169, 369 1186 Zea mays PRT G867,G1930 169, 369 1187 Glycine max DNA G869 171 1188 Glycine max DNA G869171 1189 Oryza sativa DNA G869 171 1190 Zea mays DNA G869 171 1191 Oryzasativa PRT G869 171 1192 Oryza sativa DNA G877 173 1193 Glycine max DNAG881 175 1194 Oryza sativa DNA G881 175 1195 Oryza sativa DNA G881 1751196 Zea mays DNA G881 175 1197 Zea mays DNA G881 175 1198 Zea mays DNAG881 175 1199 Zea mays DNA G881 175 1200 Oryza sativa PRT G881 175 1201Oryza sativa PRT G892 177 1202 Mentha x piperita DNA G896 179 1203Glycine max DNA G910 181 1204 Glycine max DNA G912 185 1205 Glycine maxDNA G912 185 1206 Glycine max DNA G912 185 1207 Glycine max DNA G912 1851208 Glycine max DNA G912 185 1209 Glycine max DNA G912 185 1210 Glycinemax DNA G912 185 1211 Oryza sativa DNA G912 185 1212 Oryza sativa DNAG912, G913 185, 187 1213 Zea mays DNA G912 185 1214 Zea mays DNA G912185 1215 Zea mays DNA G912, G913 185, 187 1216 Zea mays DNA G912 1851217 Zea mays DNA G912 185 1218 Brassica napus DNA G912, G913 185, 1871219 Solanum tuberosum DNA G912 185 1220 Descurainia sophia DNA G912 1851221 Oryza sativa PRT G912 185 1222 Oryza sativa PRT G912, G913 185, 1871223 Oryza sativa PRT G912, G913 185, 187 1224 Oryza sativa PRT G912 1851225 Brassica napus PRT G912 185 1226 Nicotiana tabacum PRT G912 1851227 Oryza sativa PRT G912 185 1228 Oryza sativa PRT G912 185 1229 Oryzasativa PRT G912 185 1230 Oryza sativa PRT G912 185 1231 Oryza sativa PRTG912 185 1232 Oryza sativa PRT G912 185 1233 Oryza sativa PRT G912 1851234 Oryza sativa PRT G912 185 1235 Oryza sativa PRT G912 185 1236 Oryzasativa PRT G912 185 1237 Glycine max PRT G912 185 1238 Glycine max PRTG912 185 1239 Glycine max PRT G912 185 1240 Glycine max PRT G912 1851241 Glycine max PRT G912 185 1242 Glycine max PRT G912 185 1243 Glycinemax PRT G912 185 1244 Zea mays PRT G912 185 1245 Zea mays PRT G912 1851246 Zea mays PRT G912 185 1247 Zea mays PRT G912 185 1248 Zea mays PRTG912 185 1249 Glycine max DNA G922 189 1250 Glycine max DNA G922 1891251 Glycine max DNA G922 189 1252 Oryza sativa DNA G922 189 1253 Oryzasativa DNA G922 189 1254 Oryza sativa PRT G922 189 1255 Oryza sativa PRTG922 189 1256 Oryza sativa PRT G922 189 1257 Oryza sativa PRT G922 1891258 Glycine max DNA G926 191 1259 Glycine max DNA G926 191 1260 Oryzasativa DNA G926 191 1261 Oryza sativa DNA G926 191 1262 Zea mays DNAG926 191 1263 Brassica napus PRT G926 191 1264 Glycine max DNA G961 1931265 Glycine max DNA G961 193 1266 Oryza sativa DNA G961 193 1267 Zeamays DNA G961 193 1268 Zea mays DNA G961 193 1269 Zea mays DNA G961 1931270 Oryza sativa PRT G961 193 1271 Glycine max DNA G974 197 1272Glycine max DNA G974 197 1273 Glycine max DNA G974 197 1274 Glycine maxDNA G974 197 1275 Glycine max DNA G974 197 1276 Glycine max DNA G974 1971277 Oryza sativa DNA G974 197 1278 Zea mays DNA G974 197 1279 Zea maysDNA G974 197 1280 Zea mays DNA G974 197 1281 Zea mays DNA G974 197 1282Lycopersicon DNA G974 197 esculentum 1283 Glycine max DNA G974 197 1284Solanum tuberosum DNA G974 197 1285 Poplar xylem DNA G974 197 1286Medicago truncatula DNA G974 197 1287 Sorghum bicolor DNA G974 197 1288Oryza sativa PRT G974 197 1289 Oryza sativa PRT G974 197 1290 Oryzasativa PRT G974 197 1291 Atriplex hortensis PRT G974 197 1292 Glycinemax DNA G975, G2583 199, 449 1293 Glycine max DNA G975, G2583 199, 4491294 Glycine max DNA G975, G2583 199, 449 1295 Glycine max DNA G975,G2583 199, 449 1296 Glycine max DNA G975, G2583 199, 449 1297 Oryzasativa DNA G975 199 1298 Oryza sativa DNA G975, G2583 199, 449 1299 Zeamays DNA G975, G2583 199, 449 1300 Zea mays DNA G975, G2583 199, 4491301 Brassica rapa DNA G975, G2583 199, 449 1302 Oryza sativa PRT G975,G2583 199, 449 1303 Glycine max DNA G979 201 1304 Glycine max DNA G979201 1305 Glycine max DNA G979 201 1306 Oryza sativa DNA G979 201 1307Zea mays DNA G979 201 1308 Zea mays DNA G979 201 1309 Zea mays DNA G979201 1310 Oryza sativa PRT G979 201 1311 Oryza sativa PRT G979 201 1312Oryza sativa PRT G979 201 1313 Oryza sativa PRT G979 201 1314 Oryzasativa PRT G979 201 1315 Glycine max DNA G987 203 1316 Glycine max DNAG987 203 1317 Glycine max DNA G987 203 1318 Glycine max DNA G987 2031319 Glycine max DNA G987 203 1320 Glycine max DNA G987 203 1321 Oryzasativa DNA G987 203 1322 Oryza sativa DNA G987 203 1323 Zea mays DNAG987 203 1324 Oryza sativa PRT G987 203 1325 Oryza sativa PRT G988 2051326 Oryza sativa PRT G988 205 1327 Capsella rubella PRT G988 205 1328Glycine max DNA G1040 207 1329 Glycine max DNA G1040 207 1330 Glycinemax DNA G1040 207 1331 Glycine max DNA G1040 207 1332 Glycine max DNAG1040 207 1333 Zea mays DNA G1040 207 1334 Zea mays DNA G1040 207 1335Zea mays DNA G1040 207 1336 Zea mays DNA G1040 207 1337 Zea mays DNAG1040 207 1338 Oryza sativa PRT G1040 207 1339 Oryza sativa PRT G1040207 1340 Glycine max DNA G1047 209 1341 Zea mays DNA G1047 209 1342Oryza sativa PRT G1047 209 1343 Oryza sativa PRT G1047 209 1344 Glycinemax DNA G1051, G1052 211, 213 1345 Glycine max DNA G1051, G1052 211, 2131346 Glycine max DNA G1051, G1052 211, 213 1347 Glycine max DNA G1051,G1052 211, 213 1348 Glycine max DNA G1051, G1052 211, 213 1349 Glycinemax DNA G1051, G1052 211, 213 1350 Glycine max DNA G1051, G1052 211, 2131351 Oryza sativa DNA G1051, G1052 211, 213 1352 Zea mays DNA G1051,G1052 211, 213 1353 Zea mays DNA G1051, G1052 211, 213 1354 Zea mays DNAG1051, G1052 211, 213 1355 Zea mays DNA G1051, G1052 211, 213 1356 Zeamays DNA G1051, G1052 211, 213 1357 Zea mays DNA G1051, G1052 211, 2131358 Zea mays DNA G1051, G1052 211, 213 1359 Oryza sativa DNA G1052 2131360 Zea mays DNA G1052 213 1361 Zea mays DNA G1052 213 1362 Oryzasativa PRT G1051, G1052 211, 213 1363 Oryza sativa PRT G1051, G1052 211,213 1364 Oryza sativa PRT G1051, G1052 211, 213 1365 Glycine max DNAG1062 215 1366 Glycine max DNA G1062 215 1367 Glycine max DNA G1062 2151368 Glycine max DNA G1062 215 1369 Oryza sativa DNA G1062 215 1370Oryza sativa DNA G1062 215 1371 Zea mays DNA G1062 215 1372 Zea mays DNAG1062 215 1373 Zea mays DNA G1062 215 1374 Zea mays DNA G1062 215 1375Zea mays DNA G1062 215 1376 Medicago truncatula DNA G1062 215 1377Lycopersicon DNA G1062 215 esculentum 1378 Oryza sativa PRT G1062 2151379 Glycine max DNA G1063, G2143 217, 413 1380 Glycine max DNA G1063,G2143 217, 413 1381 Glycine max DNA G1063, G2143 217, 413 1382 Glycinemax DNA G1063, G2143 217, 413 1383 Glycine max DNA G1063, G2143 217, 4131384 Lycopersicon DNA G1063, G2143 217, 413 esculentum 1385 Glycine maxDNA G1064 219 1386 Glycine max DNA G1064 219 1387 Glycine max DNA G1064219 1388 Zea mays DNA G1064 219 1389 Zea mays DNA G1064 219 1390Lycopersicon DNA G1064 219 esculentum 1391 Oryza sativa PRT G1064 2191392 Gossypium hirsutum PRT G1064 219 1393 Glycine max DNA G1069 2211394 Glycine max DNA G1069 221 1395 Oryza sativa PRT G1069, G1073 221,223 1396 Zea mays DNA G1069 221 1397 Lotus japonicus DNA G1069 221 1398Lycopersicon DNA G1073 223 esculentum 1399 Oryza sativa PRT G1073 2231400 Oryza sativa PRT G1073 223 1401 Oryza sativa PRT G1073 223 1402Oryza sativa PRT G1073 223 1403 Oryza sativa PRT G1073 223 1404 Oryzasativa PRT G1073 223 1405 Oryza sativa PRT G1073 223 1406 Oryza sativaPRT G1073 223 1407 Oryza sativa PRT G1073 223 1408 Oryza sativa PRTG1073 223 1409 Oryza sativa PRT G1073 223 1410 Oryza sativa PRT G1073223 1411 Glycine max PRT G1073 223 1412 Glycine max PRT G1073 223 1413Glycine max PRT G1073 223 1414 Glycine max PRT G1073 223 1415 Glycinemax PRT G1073 223 1416 Glycine max PRT G1073 223 1417 Glycine max PRTG1073 223 1418 Zea mays PRT G1073 223 1419 Glycine max DNA G1075 2251420 Glycine max DNA G1075 225 1421 Glycine max DNA G1075 225 1422Glycine max DNA G1075 225 1423 Glycine max DNA G1075 225 1424 Oryzasativa DNA G1075 225 1425 Oryza sativa DNA G1075 225 1426 Oryza sativaDNA G1075 225 1427 Oryza sativa DNA G1089 229 1428 Zea mays DNA G1089229 1429 Zea mays DNA G1089 229 1430 Zea mays DNA G1089 229 1431 Zeamays DNA G1089 229 1432 Zea mays DNA G1089 229 1433 Oryza sativa PRTG1089 229 1434 Glycine max DNA G1134, G2555 231, 445 1435 Glycine maxDNA G1134, G2555 231, 445 1436 Oryza sativa DNA G1134, G2555 231, 4451437 Glycine max DNA G1140 233 1438 Glycine max DNA G1140 233 1439Glycine max DNA G1140 233 1440 Glycine max DNA G1140 233 1441 Glycinemax DNA G1140 233 1442 Glycine max DNA G1140 233 1443 Oryza sativa DNAG1140 233 1444 Zea mays DNA G1140 233 1445 Zea mays DNA G1140 233 1446Zea mays DNA G1140 233 1447 Zea mays DNA G1140 233 1448 Zea mays DNAG1140 233 1449 Zea mays DNA G1140 233 1450 Zea mays DNA G1140 233 1451Zea mays DNA G1140 233 1452 Zea mays DNA G1140 233 1453 Oryza sativa PRTG1140 233 1454 Ipomoea batatas PRT G1140 233 1455 Zea mays DNA G1146 2371456 Zea mays DNA G1146 237 1457 Oryza sativa PRT G1146 237 1458 Oryzasativa PRT G1146 237 1459 Oryza sativa PRT G1146 237 1460 Glycine maxDNA G1196 239 1461 Glycine max DNA G1196 239 1462 Glycine max DNA G1196239 1463 Oryza sativa DNA G1196 239 1464 Zea mays DNA G1196 239 1465 Zeamays DNA G1196 239 1466 Oryza sativa PRT G1196 239 1467 Oryza sativa PRTG1196 239 1468 Glycine max DNA G1198 241 1469 Glycine max DNA G1198 2411470 Glycine max DNA G1198 241 1471 Glycine max DNA G1198 241 1472Glycine max DNA G1198 241 1473 Glycine max DNA G1198 241 1474 Glycinemax DNA G1198 241 1475 Glycine max DNA G1198 241 1476 Oryza sativa DNAG1198 241 1477 Oryza sativa DNA G1198 241 1478 Oryza sativa DNA G1198241 1479 Oryza sativa DNA G1198 241 1480 Oryza sativa DNA G1198 241 1481Zea mays DNA G1198 241 1482 Zea mays DNA G1198 241 1483 Zea mays DNAG1198 241 1484 Zea mays DNA G1198 241 1485 Zea mays DNA G1198 241 1486Zea mays DNA G1198 241 1487 Zea mays DNA G1198 241 1488 Zea mays DNAG1198 241 1489 Zea mays DNA G1198 241 1490 Zea mays DNA G1198 241 1491Nicotiana tabacum DNA G1198 241 1492 Oryza sativa PRT G1198 241 1493Oryza sativa PRT G1198 241 1494 Oryza sativa PRT G1198 241 1495 Oryzasativa PRT G1198 241 1496 Oryza sativa PRT G1198 241 1497 Oryza sativaPRT G1198 241 1498 Oryza sativa PRT G1198 241 1499 Zea mays DNA G1225243 1500 Oryza sativa PRT G1225 243 1501 Oryza sativa PRT G1226 245 1502Glycine max DNA G1229 247 1503 Oryza sativa PRT G1229 247 1504 Oryzasativa PRT G1229 247 1505 Glycine max DNA G1255 249 1506 Glycine max DNAG1255 249 1507 Glycine max DNA G1255 249 1508 Glycine max DNA G1255 2491509 Glycine max DNA G1255 249 1510 Glycine max DNA G1255 249 1511Glycine max DNA G1255 249 1512 Oryza sativa DNA G1255 249 1513 Oryzasativa DNA G1255 249 1514 Oryza sativa DNA G1255 249 1515 Oryza sativaDNA G1255 249 1516 Zea mays DNA G1255 249 1517 Zea mays DNA G1255 2491518 Zea mays DNA G1255 249 1519 Zea mays DNA G1255 249 1520 Zea maysDNA G1255 249 1521 Zea mays DNA G1255 249 1522 Oryza sativa PRT G1255249 1523 Glycine max DNA G1266 251 1524 Glycine max DNA G1266 251 1525Glycine max DNA G1266 251 1526 Glycine max DNA G1266 251 1527 Oryzasativa DNA G1266 251 1528 Nicotiana tabacum PRT G1266 251 1529 Oryzasativa DNA G1275 253 1530 Zea mays DNA G1275 253 1531 Zea mays DNA G1275253 1532 Zea mays DNA G1275 253 1533 Oryza sativa PRT G1275 253 1534Oryza sativa PRT G1275 253 1535 Oryza sativa PRT G1275 253 1536 Glycinemax DNA G1322 257 1537 Glycine max DNA G1322 257 1538 Glycine max DNAG1322 257 1539 Oryza sativa DNA G1322 257 1540 Oryza sativa PRT G1322257 1541 Oryza sativa PRT G1322 257 1542 Zea mays DNA G1323 259 1543 Zeamays DNA G1323 259 1544 Glycine max DNA G1330 261 1545 Glycine max DNAG1330 261 1546 Glycine max DNA G1330 261 1547 Glycine max DNA G1330 2611548 Glycine max DNA G1330 261 1549 Glycine max DNA G1330 261 1550Glycine max DNA G1330 261 1551 Oryza sativa DNA G1330 261 1552 Oryzasativa DNA G1330 261 1553 Oryza sativa DNA G1330 261 1554 Oryza sativaDNA G1330 261 1555 Zea mays DNA G1330 261 1556 Zea mays DNA G1330 2611557 Zea mays DNA G1330 261 1558 Zea mays DNA G1330 261 1559 Zea maysDNA G1330 261 1560 Zea mays DNA G1330 261 1561 Zea mays DNA G1330 2611562 Lycopersicon DNA G1330 261 esculentum 1563 Oryza sativa PRT G1330261 1564 Oryza sativa PRT G1330 261 1565 Oryza sativa PRT G1330 261 1566Oryza sativa PRT G1330 261 1567 Glycine max DNA G1331 263 1568 Glycinemax DNA G1331 263 1569 Oryza sativa DNA G1331 263 1570 Zea mays DNAG1331 263 1571 Zea mays DNA G1331 263 1572 Oryza sativa PRT G1331 2631573 Glycine max DNA G1363 267 1574 Oryza sativa DNA G1363 267 1575Oryza sativa DNA G1363 267 1576 Oryza sativa DNA G1363 267 1577 Oryzasativa DNA G1363 267 1578 Zea mays DNA G1363 267 1579 Zea mays DNA G1363267 1580 Zea mays DNA G1363 267 1581 Zea mays DNA G1363 267 1582 Zeamays DNA G1363 267 1583 Oryza sativa PRT G1363 267 1584 Oryza sativa PRTG1363 267 1585 Oryza sativa PRT G1363 267 1586 Oryza sativa PRT G1363267 1587 Glycine max DNA G1411, G2509 269, 439 1588 Glycine max DNAG1411, G2509 269, 439 1589 Glycine max DNA G1411, G2509 269, 439 1590Glycine max DNA G1411, G2509 269, 439 1591 Zea mays DNA G1411, G2509269, 439 1592 Glycine max DNA G1417 271 1593 Oryza sativa PRT G1417 2711594 Oryza sativa PRT G1417 271 1595 Glycine max DNA G1419 273 1596Glycine max DNA G1449 275 1597 Glycine max DNA G1449 275 1598 Oryzasativa DNA G1449 275 1599 Oryza sativa DNA G1449 275 1600 Zea mays DNAG1449 275 1601 Zea mays DNA G1449 275 1602 Zea mays DNA G1449 275 1603Zea mays DNA G1449 275 1604 Glycine max DNA G1451 277 1605 Glycine maxDNA G1451 277 1606 Oryza sativa DNA G1451 277 1607 Oryza sativa DNAG1451 277 1608 Oryza sativa DNA G1451 277 1609 Zea mays DNA G1451 2771610 Zea mays DNA G1451 277 1611 Zea mays DNA G1451 277 1612 Zea maysDNA G1451 277 1613 Medicago truncatula DNA G1451 277 1614 Solanumtuberosum DNA G1451 277 1615 Zea mays DNA G1451 277 1616 Sorghum DNAG1451 277 propinquum 1617 Glycine max DNA G1451 277 1618 Sorghum bicolorDNA G1451 277 1619 Hordeum vulgare DNA G1451 277 1620 Lycopersicon DNAG1451 277 esculentum 1621 Oryza sativa PRT G1451 277 1622 Oryza sativaPRT G1451 277 1623 Oryza sativa PRT G1451 277 1624 Oryza sativa PRTG1451 277 1625 Glycine max DNA G1452 279 1626 Glycine max DNA G1478 2851627 Glycine max DNA G1478 285 1628 Glycine max DNA G1478 285 1629 Zeamays DNA G1478 285 1630 Glycine max DNA G1482 287 1631 Glycine max DNAG1482 287 1632 Glycine max DNA G1482 287 1633 Glycine max DNA G1482 2871634 Glycine max DNA G1482 287 1635 Oryza sativa DNA G1482 287 1636Oryza sativa DNA G1482 287 1637 Oryza sativa DNA G1482 287 1638 Oryzasativa DNA G1482 287 1639 Zea mays DNA G1482 287 1640 Zea mays DNA G1482287 1641 Zea mays DNA G1482 287 1642 Zea mays DNA G1482 287 1643 Zeamays DNA G1482 287 1644 Zea mays DNA G1482 287 1645 Oryza sativa PRTG1482 287 1646 Oryza sativa PRT G1482 287 1647 Glycine max DNA G1488 2891648 Glycine max DNA G1488 289 1649 Glycine max DNA G1488 289 1650 Oryzasativa DNA G1488 289 1651 Oryza sativa DNA G1488 289 1652 Zea mays DNAG1488 289 1653 Zea mays DNA G1488 289 1654 Zea mays DNA G1488 289 1655Oryza sativa PRT G1488 289 1656 Oryza sativa PRT G1488 289 1657 Oryzasativa PRT G1488 289 1658 Oryza sativa PRT G1499 295 1659 Brassica rapasubsp. DNA G1499 295 pekinensis 1660 Glycine max DNA G1519 297 1661Oryza sativa DNA G1519 297 1662 Zea mays DNA G1519 297 1663 Zea mays DNAG1519 297 1664 Lycopersicon DNA G1519 297 esculentum 1665 Glycine maxDNA G1526 2199 1666 Oryza sativa DNA G1526 299 1667 Oryza sativa DNAG1526 299 1668 Zea mays DNA G1526 299 1669 Glycine max DNA G1540 3011670 Oryza sativa PRT G1540 301 1671 Glycine max DNA G1543 303 1672Oryza sativa DNA G1543 303 1673 Zea mays DNA G1543 303 1674 Oryza sativaPRT G1543 303 1675 Zea mays DNA G1637 307 1676 Zea mays DNA G1637 3071677 Zea mays DNA G1637 307 1678 Glycine max DNA G1640 309 1679 Glycinemax DNA G1640 309 1680 Glycine max DNA G1640 309 1681 Oryza sativa PRTG1640 309 1682 Zea mays DNA G1645 311 1683 Zea mays DNA G1645 311 1684Zea mays DNA G1645 311 1685 Lycopersicon DNA G1645 311 esculentum 1686Medicago truncatula DNA G1645 311 1687 Oryza sativa PRT G1645 311 1688Oryza sativa DNA G1646 313 1689 Oryza sativa DNA G1646 313 1690 Glycinemax DNA G1652 315 1691 Glycine max DNA G1652 315 1692 Glycine max DNAG1652 315 1693 Glycine max DNA G1652 315 1694 Glycine max DNA G1652 3151695 Glycine max DNA G1652 315 1696 Glycine max DNA G1652 315 1697Glycine max DNA G1652 315 1698 Oryza sativa DNA G1652 315 1699 Zea maysDNA G1652 315 1700 Zea mays DNA G1652 315 1701 Oryza sativa PRT G1652315 1702 Oryza sativa PRT G1652 315 1703 Oryza sativa PRT G1652 315 1704Oryza sativa PRT G1652 315 1705 Oryza sativa PRT G1652 315 1706 Glycinemax DNA G1672 317 1707 Oryza sativa DNA G1672 317 1708 Zea mays DNAG1672 317 1709 Zea mays DNA G1672 317 1710 Oryza sativa PRT G1672 3171711 Oryza sativa PRT G1672 317 1712 Oryza sativa PRT G1672 317 1713Oryza sativa PRT G1672 317 1714 Glycine max DNA G1750 323 1715 Glycinemax DNA G1750 323 1716 Glycine max DNA G1750 323 1717 Glycine max DNAG1750 323 1718 Oryza sativa DNA G1750 323 1719 Zea mays DNA G1750 3231720 Zea mays DNA G1750 323 1721 Glycine max DNA G1756 325 1722 Medicagotruncatula DNA G1765 327 1723 Glycine max DNA G1777 329 1724 Oryzasativa DNA G1777 329 1725 Zea mays DNA G1777 329 1726 Zea mays DNA G1777329 1727 Oryza sativa PRT G1777 329 1728 Glycine max DNA G1792 331 1729Glycine max DNA G1792 331 1730 Glycine max DNA G1792 331 1731 Glycinemax DNA G1792 331 1732 Glycine max DNA G1792 331 1733 Zea mays DNA G1792331 1734 Lycopersicon DNA G1792 331 esculentum 1735 G3380 Oryza sativaPRT G1792 331 1736 G3381 Oryza sativa indica PRT G1792 331 1737 G3383Oryza sativa japonica PRT G1792 331 1738 Glycine max DNA G1793 333 1739Oryza sativa DNA G1793 333 1740 Zea mays DNA G1793 333 1741 Zea mays DNAG1793 333 1742 Zea mays DNA G1793 333 1743 Oryza sativa PRT G1793 3331744 Glycine max DNA G1794 335 1745 Glycine max DNA G1794 335 1746Glycine max DNA G1794 335 1747 Glycine max DNA G1794 335 1748 Glycinemax DNA G1794 335 1749 Glycine max DNA G1794 335 1750 Glycine max DNAG1794 335 1751 Zea mays DNA G1794 335 1752 Zea mays DNA G1794 335 1753Zea mays DNA G1794 335 1754 Oryza sativa PRT G1794 335 1755 Oryza sativaPRT G1794 335 1756 Oryza sativa PRT G1794 335 1757 Glycine max DNA G1804337 1758 Glycine max DNA G1804 337 1759 Glycine max DNA G1804 337 1760Oryza sativa DNA G1804 337 1761 Oryza sativa PRT G1804 337 1762Helianthus annuus PRT G1804 337 1763 Glycine max DNA G1838 345 1764Glycine max DNA G1838 345 1765 Oryza sativa PRT G1838 345 1766 Glycinemax DNA G1841 347 1767 Glycine max DNA G1841 347 1768 Oryza sativa DNAG1841 347 1769 Oryza sativa PRT G1841 347 1770 Solanum tuberosum DNAG1852 353 1771 Gossypium arboreum DNA G1852 353 1772 Medicago truncatulaDNA G1852 353 1773 Glycine max DNA G1852 353 1774 Lycopersicon DNA G1852353 esculentum 1775 Pinus taeda DNA G1852 353 1776 Lotus japonicus DNAG1852 353 1777 Gossypium hirsutum DNA G1852 353 1778 Solanum tuberosumDNA G1863 355 1779 Medicago truncatula DNA G1863 355 1780 LycopersiconDNA G1863 355 esculentum 1781 Oryza sativa PRT G1863 355 1782 Glycinemax DNA G1880 357 1783 Glycine max DNA G1880 357 1784 Medicagotruncatula DNA G1880 357 1785 Oryza sativa PRT G1880 357 1786 Glycinemax DNA G1902 361 1787 Glycine max DNA G1902 361 1788 Glycine max DNAG1902 361 1789 Zea mays DNA G1902 361 1790 Oryza sativa PRT G1902 3611791 Glycine max DNA G1927 367 1792 Oryza sativa DNA G1927 367 1793 Zeamays DNA G1927 367 1794 Lycopersicon DNA G1927 367 esculentum 1795 Oryzasativa DNA G1930 369 1796 Glycine max DNA G1944 373 1797 Glycine max DNAG1944 373 1798 Zea mays DNA G1944 373 1799 Glycine max DNA G1944 3731800 Glycine max DNA G1944 373 1801 Glycine max DNA G1946 375 1802Glycine max DNA G1946 375 1803 Zea mays DNA G1946 375 1804 Zea mays DNAG1946 375 1805 Oryza sativa PRT G1946 375 1806 Glycine max DNA G1948 3791807 Glycine max DNA G1948 379 1808 Oryza sativa DNA G1948 379 1809Oryza sativa DNA G1948 379 1810 Zea mays DNA G1948 379 1811 Zea mays DNAG1948 379 1812 Zea mays DNA G1948 379 1813 Oryza sativa PRT G1948 3791814 Glycine max DNA G1950 381 1815 Glycine max DNA G1950 381 1816Glycine max DNA G1950 381 1817 Glycine max DNA G1950 381 1818 Glycinemax DNA G1950 381 1819 Glycine max DNA G1950 381 1820 Oryza sativa DNAG1950 381 1821 Oryza sativa DNA G1950 381 1822 Oryza sativa DNA G1950381 1823 Oryza sativa DNA G1950 381 1824 Oryza sativa DNA G1950 381 1825Oryza sativa DNA G1950 381 1826 Oryza sativa DNA G1950 381 1827 Oryzasativa DNA G1950 381 1828 Oryza sativa DNA G1950 381 1829 Zea mays DNAG1950 381 1830 Zea mays DNA G1950 381 1831 Zea mays DNA G1950 381 1832Zea mays DNA G1950 381 1833 Zea mays DNA G1950 381 1834 Zea mays DNAG1950 381 1835 Zea mays DNA G1950 381 1836 Zea mays DNA G1950 381 1837Zea mays DNA G1950 381 1838 Oryza sativa PRT G1950 381 1839 Oryza sativaPRT G1950 381 1840 Oryza sativa PRT G1950 381 1841 Oryza sativa PRTG1950 381 1842 Oryza sativa PRT G1950 381 1843 Oryza sativa PRT G1950381 1844 Oryza sativa PRT G1950 381 1845 Oryza sativa PRT G1950 381 1846Oryza sativa PRT G1950 381 1847 Glycine max DNA G1958 383 1848 Glycinemax DNA G1958 383 1849 Glycine max DNA G1958 383 1850 Glycine max DNAG1958 383 1851 Glycine max DNA G1958 383 1852 Oryza sativa DNA G1958 3831853 Oryza sativa DNA G1958 383 1854 Zea mays DNA G1958 383 1855 Zeamays DNA G1958 383 1856 Zea mays DNA G1958 383 1857 Nicotiana tabacumPRT G1958 383 1858 Glycine max DNA G2007 385 1859 Glycine max DNA G2007385 1860 Zea mays DNA G2007 385 1861 Zea mays DNA G2007 385 1862 Zeamays DNA G2007 385 1863 Oryza sativa PRT G2007 385 1864 Glycine max DNAG2010, G2347 387, 431 1865 Oryza sativa DNA G2010, G2347 387, 431 1866Zea mays DNA G2010 387 1867 Zea mays DNA G2010, G2347 387, 431 1868Glycine max DNA G2059 391 1869 Glycine max DNA G2085 393 1870 Glycinemax DNA G2085 393 1871 Glycine max DNA G2085 393 1872 Glycine max DNAG2085 393 1873 Zea mays DNA G2085 393 1874 Oryza sativa PRT G2085 3931875 Oryza sativa PRT G2105 395 1876 Glycine max DNA G2110 397 1877Oryza sativa DNA G2114 399 1878 Oryza sativa DNA G2114 399 1879 Zea maysDNA G2114 399 1880 Zea mays DNA G2114 399 1881 Oryza sativa DNA G2117401 1882 Medicago truncatula DNA G2130 405 1883 Oryza sativa PRT G2130405 1884 Oryza sativa PRT G2130 405 1885 Glycine max DNA G2140 411 1886Glycine max DNA G2140 411 1887 Glycine max DNA G2140 411 1888 Glycinemax DNA G2140 411 1889 Glycine max DNA G2140 411 1890 Glycine max DNAG2140 411 1891 Oryza sativa DNA G2140 411 1892 Oryza sativa DNA G2140411 1893 Oryza sativa DNA G2140 411 1894 Oryza sativa DNA G2140 411 1895Zea mays DNA G2140 411 1896 Lycopersicon DNA G2140 411 esculentum 1897Oryza sativa PRT G2140 411 1898 Oryza sativa PRT G2140 411 1899 Oryzasativa PRT G2140 411 1900 Oryza sativa PRT G2140 411 1901 Oryza sativaPRT G2140 411 1902 Glycine max DNA G2143 413 1903 Glycine max DNA G2143413 1904 Glycine max DNA G2144 415 1905 Glycine max DNA G2144 415 1906Zea mays DNA G2144 415 1907 Zea mays DNA G2144 415 1908 Medicagotruncatula DNA G2155 419 1909 Medicago truncatula DNA G2155 419 1910Glycine max DNA G2155 419 1911 Oryza sativa PRT G2192 421 1912 Oryzasativa PRT G2295 423 1913 Glycine max DNA G2340 425 1914 Glycine max DNAG2343 427 1915 Glycine max DNA G2343 427 1916 Glycine max DNA G2343 4271917 Lycopersicon PRT G2343 427 esculentum 1918 Oryza sativa PRT G2379433 1919 Oryza sativa PRT G2379 433 1920 Oryza sativa PRT G2379 433 1921Glycine max DNA G2505 437 1922 Zea mays DNA G2505 437 1923 Glycine maxDNA G2520 443 1924 Glycine max DNA G2520 443 1925 Oryza sativa DNA G2520443 1926 Zea mays DNA G2520 443 1927 Zea mays DNA G2520 443 1928 Zeamays DNA G2520 443 1929 Oryza sativa PRT G2520 443 1930 Oryza sativa PRTG2520 443 1931 Glycine max DNA G2557 447 1932 Glycine max DNA G2557 4471933 Glycine max DNA G2557 447 1934 Zea mays DNA G2557 447 1935 Zea maysDNA G2557 447 1936 Glycine max DNA G2557 447 1937 Oryza sativa PRT G2557447 1938 Oryza sativa PRT G2557 447 1939 Oryza sativa PRT G2557 447 1940Glycine max DNA G2719 453 1941 Zea mays DNA G2719 453 1942 Oryza sativaPRT G2719 453 1943 Oryza sativa PRT G2719 453 1944 Glycine max DNA G2789455 1945 Medicago truncatula DNA G2789 455 1946 Glycine max DNA G2830457

Table 8 lists a summary of homologous sequences identified using BLAST(tblastx program). The first column shows the polynucleotide sequenceidentifier (SEQ ID NO), the second column shows the corresponding cDNAidentifier (Gene ID), the third column shows the orthologous orhomologous polynucleotide GenBank Accession Number (Test Sequence ID),the fourth column shows the calculated probability value that thesequence identity is due to chance (Smallest Sum Probability), the fifthcolumn shows the plant species from which the test sequence was isolated(Test Sequence Species), and the sixth column shows the orthologous orhomologous test sequence GenBank annotation (Test Sequence GenBankAnnotation).

TABLE 8 Summary of representative sequences that are homologous topresently-disclosed transcription factors Poly- nucleotide Smallest SEQSum Test Sequence GenBank ID NO: GID Test Sequence ID Probability TestSequence Species Annotation 1 G8 AF134116 2.00E−92 Hyacinthus orientalisAPETALA2 protein homolog HAP2 (HAP2) 1 G8 AF132002 6.00E−86 Petunia xhybrida PHAP2B protein (Ap2B) mRNA, complete cds. 1 G8 AF332215 8.00E−84Malus x domestica transcription factor AHAP2 (AHAP2) mRNA, 1 G8 CA7837943.00E−83 Glycine max sat57d09.y1 Gm-c1056 Glycine max cDNA clone SOY 1G8 AY069953 7.00E−82 Hordeum vulgare APETALA2-like protein (AP2L1) mRNA,complet 1 G8 AF253971 5.00E−81 Picea abies APETALA2-relatedtranscription factor 2 (AP2L2) 1 G8 AF048900 2.00E−80 Zea maysindeterminate spikelet 1 (ids1) mRNA, complete cds 1 G8 AF3255064.00E−80 Pisum sativum APETAL2-like protein mRNA, complete cds. 1 G8BG321674 6.00E−79 Descurainia sophia Ds01_06a02_ADs01_AAFC_ECORC_cold_stress 1 G8 BQ120583 3.00E−78 Solanum tuberosumEST606159 mixed potato tissues Solanum tu 1 G8 gi24059986 1.30E−91 Oryzasativa (japonica putative indetermi cultivar-group) 1 G8 gi53609968.70E−88 Hyacinthus orientalis APETALA2 protein homolog HAP2. 1 G8gi5081555 4.50E−86 Petunia x hybrida PHAP2A protein. 1 G8 gi29440405.80E−84 Zea mays indeterminate spikelet 1. 1 G8 gi21717332 9.30E−82Malus x domestica transcription factor AHAP2. 1 G8 gi11181612 7.50E−78Picea abies APETALA2-related transcription factor 2. 1 G8 gi131731641.60E−77 Pisum sativum APETAL2-like protein. 1 G8 gi18476518 2.60E−70Hordeum vulgare APETALA2-like protein. 1 G8 gi21069051 1.40E−34 Brassicanapus AP2/EREBP transcription factor BABY BOOM1. 1 G8 gi213042258.60E−33 Oryza sativa aintegumenta-like protein. 3 G19 BG3213581.00E−101 Descurainia sophia Ds01_07d03_R Ds01_AAFC_ECORC_cold_stress 3G19 BH444831 1.00E−77 Brassica oleracea BOHPW42TR BOHP Brassica oleraceagenomic 3 G19 BM412184 2.00E−43 Lycopersicon EST586511 tomato breakeresculentum fruit Lyco 3 G19 BU837697 3.00E−43 Populus tremula x T104G02Populus apica Populus tremuloides 3 G19 CA784650 6.00E−43 Glycine maxsat87a10.y1 Gm-c1062 Glycine max cDNA clone SOY 3 G19 BU819833 3.00E−41Populus tremula UA48BPB07 Populus tremula cambium cDNA libr 3 G19BU870388 4.00E−41 Populus balsamifera Q011H05 Populus flow subsp.trichocarpa 3 G19 CA797119 1.00E−38 Theobroma cacao Cac_BL_4204 Cac_BL(Bean and Leaf from Amel 3 G19 BI436183 2.00E−38 Solanum tuberosumEST538944 cSTE Solanum tuberosum cDNA clo 3 G19 BQ989448 2.00E−36Lactuca sativa QGF17L05.yg.ab1 QG_EFGHJ lettuce serriola La 3 G19gi10798644 5.70E−36 Nicotiana tabacum AP2 domain-containingtranscription fac 3 G19 gi6176534 2.40E−35 Oryza sativa EREBP-likeprotein. 3 G19 gi1688233 7.50E−34 Solanum tuberosum DNA binding proteinhomolog. 3 G19 gi22074046 1.50E−33 Lycopersicon transcription factorJERF1. esculentum 3 G19 gi18496063 4.90E−33 Fagus sylvatica ethyleneresponsive element binding prote 3 G19 gi20805105 2.10E−32 Oryza sativa(japonica contains ESTs AU06 cultivar-group) 3 G19 gi24940524 2.30E−31Triticum aestivum ethylene response element binding prote 3 G19gi18266198 2.30E−31 Narcissus AP-2 domain containing pseudonarcissusprotein. 3 G19 gi3264767 1.30E−30 Prunus armeniaca AP2 domain containingprotein. 3 G19 gi24817250 4.00E−28 Cicer arietinum transcription factorEREBP- like protein. 5 G22 AB016264 9.00E−48 Nicotiana sylvestris nserf2gene for ethylene- responsive el 5 G22 TOBBY4A 1.00E−47 Nicotianatabacum mRNA for ERF1, complete cds. 5 G22 AP004533 4.00E−47 Lotusjaponicus genomic DNA, chromosome 3, clone: LjT14G02, 5 G22 LEU892556.00E−47 Lycopersicon DNA-binding protein Pti4 esculentum mRNA, comp 5G22 BQ517082 6.00E−46 Solanum tuberosum EST624497 Generation of a set ofpotato c 5 G22 BE449392 1.00E−45 Lycopersicon hirsutum EST356151 L.hirsutum trichome, Corne 5 G22 AF245119 5.00E−45 MesembryanthemumAP2-related transcription crystallinum fac 5 G22 BQ165291 7.00E−45Medicago truncatula EST611160 KVKC Medicago truncatula cDNA 5 G22AW618245 8.00E−38 Lycopersicon pennellii EST314295 L. pennelliitrichome, Cor 5 G22 BG444654 2.00E−36 Gossypium arboreum GA_Ea0025B11fGossypium arboreum 7-10 d 5 G22 gi1208495 6.10E−48 Nicotiana tabacumERF1. 5 G22 gi3342211 3.30E−47 Lycopersicon Pti4. esculentum 5 G22gi8809571 8.90E−47 Nicotiana sylvestris ethylene-responsive elementbinding 5 G22 gi17385636 2.70E−36 Matricaria chamomillaethylene-responsive element binding 5 G22 gi8980313 2.50E−33Catharanthus roseus AP2-domain DNA-binding protein. 5 G22 gi75282768.60E−33 Mesembryanthemum AP2-related transcription f crystallinum 5 G22gi21304712 3.10E−28 Glycine max ethylene-responsive element bindingprotein 1 5 G22 gi14140141 1.50E−26 Oryza sativa putative AP2-relatedtranscription factor. 5 G22 gi15623863 1.30E−22 Oryza sativa (japonicacontains EST~hypot cultivar-group) 5 G22 gi4099914 3.10E−21 Stylosantheshamata ethylene-responsive element binding p 7 G24 BZ026790 7.00E−71Brassica oleracea oeh27a09.b1 B. oleracea002 Brassica olerac 7 G24BM985484 4.00E−52 Thellungiella halophila 10_C12_T Ath Thellungiellahalophil 7 G24 BQ405872 3.00E−45 Gossypium arboreum GA_Ed0088A03fGossypium arboreum 7-10 d 7 G24 BG543187 3.00E−44 Brassica rapa subsp.E0677 Chinese cabbage pekinensis etiol 7 G24 AW981184 7.00E−42 Medicagotruncatula EST392378 DSIL Medicago truncatula cDNA 7 G24 BQ7042899.00E−41 Brassica napus Bn01_04f19_A 7 G24 BG321374 9.00E−40 Descurainiasophia Ds01_06d08_R Ds01_AAFC_ECORC_cold_stress 7 G24 OSIG00036 4.00E−37Oryza sativa chromosome 4 clone H0721B11, *** SEQUENCING I 7 G24AAAA01024762 4.00E−37 Oryza sativa (indica ( ) scaffold024762cultivar-group) 7 G24 BQ586795 6.00E−37 Beta vulgarisE012390-024-012-J13-SP6 MPIZ-ADIS-024-leaf Be 7 G24 gi5091503 9.60E−34Oryza sativa EST AU055776(S20048) corresponds to a region 7 G24gi20161239 6.40E−21 Oryza sativa (japonica hypothetical protecultivar-group) 7 G24 gi8980313 2.20E−20 Catharanthus roseus AP2-domainDNA-binding protein. 7 G24 gi4099921 2.80E−20 Stylosanthes hamataEREBP-3 homolog. 7 G24 gi10798644 5.70E−20 Nicotiana tabacum AP2domain-containing transcription fac 7 G24 gi8571476 1.70E−18 Atriplexhortensis apetala2 domain-containing protein. 7 G24 gi8809573 2.10E−18Nicotiana sylvestris ethylene-responsive element binding 7 G24gi21908034 2.20E−18 Zea mays DRE binding factor 2. 7 G24 gi173522839.60E−18 Brassica napus CBF-like protein. 7 G24 gi3342211 4.70E−17Lycopersicon Pti4. esculentum 9 G28 AF245119 2.00E−72 MesembryanthemumAP2-related transcription crystallinum fac 9 G28 BQ165291 1.00E−68Medicago truncatula EST611160 KVKC Medicago truncatula cDNA 9 G28AB016264 1.00E−57 Nicotiana sylvestris nserf2 gene for ethylene-responsive el 9 G28 TOBBY4D 2.00E−57 Nicotiana tabacum Tobacco mRNA forEREBP-2, complete cds. 9 G28 BQ047502 2.00E−57 Solanum tuberosumEST596620 P. infestans- challenged potato 9 G28 LEU89255 2.00E−56Lycopersicon DNA-binding protein Pti4 esculentum mRNA, comp 9 G28BH454277 2.00E−54 Brassica oleracea BOGSI45TR BOGS Brassica oleraceagenomic 9 G28 BE449392 1.00E−53 Lycopersicon hirsutum EST356151 L.hirsutum trichome, Corne 9 G28 AB035270 2.00E−50 Matricaria chamomillaMcEREBP1 mRNA for ethylene-responsive 9 G28 AW233956 5.00E−50 Glycinemax sf32e02.y1 Gm-c1028 Glycine max cDNA clone GENO 9 G28 gi75282766.10E−71 Mesembryanthemum AP2-related transcription f crystallinum 9 G28gi8809571 3.30E−56 Nicotiana sylvestris ethylene-responsive elementbinding 9 G28 gi3342211 4.20E−56 Lycopersicon Pti4. esculentum 9 G28gi1208498 8.70E−56 Nicotiana tabacum EREBP-2. 9 G28 gi14140141 4.20E−49Oryza sativa putative AP2-related transcription factor. 9 G28 gi173856363.00E−46 Matricaria chamomilla ethylene-responsive element binding 9 G28gi21304712 2.90E−31 Glycine max ethylene-responsive element bindingprotein 1 9 G28 gi15623863 5.60E−29 Oryza sativa (japonica containsEST~hypot cultivar-group) 9 G28 gi8980313 1.20E−26 Catharanthus roseusAP2-domain DNA-binding protein. 9 G28 gi4099921 3.10E−21 Stylosantheshamata EREBP-3 homolog. 11 G47 BG543936 1.00E−60 Brassica rapa subsp.E1686 Chinese cabbage pekinensis etiol 11 G47 BH420519 3.00E−43 Brassicaoleracea BOGUH88TF BOGU Brassica oleracea genomic 11 G47 AU2926033.00E−30 Zinnia elegans AU292603 zinnia cultured mesophyll cell equa 11G47 BE320193 1.00E−24 Medicago truncatula NF024B04RT1F1029 Developingroot Medica 11 G47 AAAA01000718 1.00E−22 Oryza sativa (indica ( )scaffold000718 cultivar-group) 11 G47 AP003379 2.00E−22 Oryza sativachromosome 1 clone P0408G07, *** SEQUENCING IN 11 G47 AC124836 8.00E−21Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 11 G47BZ403609 2.00E−20 Zea mays OGABN17TM ZM_0.7_1.5_KB Zea mays genomicclone ZMM 11 G47 BM112772 6.00E−17 Solanum tuberosum EST560308 potatoroots Solanum tuberosum 11 G47 BQ698717 1.00E−16 Pinus taedaNXPV_148_C06_F NXPV (Nsf Xylem Planings wood Ve 11 G47 gi201612396.90E−24 Oryza sativa (japonica hypothetical prote cultivar-group) 11G47 gi14140155 6.80E−17 Oryza sativa putative AP2 domain transcriptionfactor. 11 G47 gi21908034 7.00E−15 Zea mays DRE binding factor 2. 11 G47gi20303011 1.90E−14 Brassica napus CBF-like protein CBF5. 11 G47gi8571476 3.00E−14 Atriplex hortensis apetala2 domain-containingprotein. 11 G47 gi8980313 2.10E−13 Catharanthus roseus AP2-domainDNA-binding protein. 11 G47 gi19071243 4.40E−13 Hordeum vulgare CRT/DREbinding factor 1. 11 G47 gi18650662 5.60E−13 Lycopersicon ethyleneresponse factor 1. esculentum 11 G47 gi17385636 1.20E−12 Matricariachamomilla ethylene-responsive element binding 11 G47 gi1208498 1.50E−12Nicotiana tabacum EREBP-2. 13 G156 AF335242 4.00E−45 Petunia x hybridaMADS-box transcription factor FBP24 (FBP2 13 G156 AMA307056 2.00E−41Antirrhinum majus mRNA for putative MADS- domain transcript 13 G156BF276751 1.00E−35 Gossypium arboreum GA_Eb0030I08f Gossypium arboreum7-10 d 13 G156 AB071380 2.00E−35 Lilium regale LRGLOB mRNA for MADS-boxtranscription factor 13 G156 ZMA271208 2.00E−34 Zea mays mRNA forputative MADS- domain transcription facto 13 G156 AI899235 1.00E−33Lycopersicon EST268678 tomato ovary, esculentum TAMU Lycope 13 G156GGN132219 8.00E−33 Gnetum gnemon mRNA for putative MADS domaintranscription 13 G156 BQ753907 2.00E−32 Hordeum vulgare subsp.EBca01_SQ002_D17_R vulgare carpel, p 13 G156 AF134114 1.00E−31Hyacinthus orientalis PISTILLATA protein homolog HPI1 (HPI1 13 G156AB094985 1.00E−30 Orchis italica OrcPI mRNA for PI/GLO- like protein,complete 13 G156 gi13384062 8.50E−42 Petunia x hybrida MADS-boxtranscription factor FBP24. 13 G156 gi19578307 2.00E−40 Antirrhinummajus putative MADS-domain transcription fact 13 G156 gi205132621.30E−36 Lilium regale MADS-box transcription factor. 13 G156 gi180762092.70E−36 Zea mays putative MADS-domain transcription factor. 13 G156gi5019464 1.40E−34 Gnetum gnemon putative MADS domain transcriptionfactor G 13 G156 gi3114586 7.10E−34 Eucalyptus grandis MADS box protein.13 G156 gi4885036 9.00E−34 Hyacinthus orientalis PISTILLATA proteinhomolog HPI2. 13 G156 gi24421111 1.60E−31 Orchis italica PI/GLO-likeprotein. 13 G156 gi2961437 2.30E−31 Oryza sativa MADS box protein. 13G156 gi16549070 3.40E−31 Magnolia praecocissima putative MADS-domaintranscription 15 G157 AY036888 1.00E−63 Brassica napus MADS-box protein(FLC1) mRNA, complete cds. 15 G157 BG596731 1.00E−37 Solanum tuberosumEST495409 cSTS Solanum tuberosum cDNA clo 15 G157 BG544805 1.00E−37Brassica rapa subsp. E2809 Chinese cabbage pekinensis etiol 15 G157AW219962 4.00E−37 Lycopersicon EST302445 tomato root esculentumduring/after 15 G157 BM436799 5.00E−36 Vitis vinifera VVA010B05_53181 Anexpressed sequence tag da 15 G157 BU875165 1.00E−31 Populus balsamiferaV003A12 Populus flow subsp. trichocarpa 15 G157 BQ868455 2.00E−31Lactuca sativa QGD14A13.yg.ab1 QG_ABCDI lettuce salinas Lac 15 G157BI957545 1.00E−30 Hordeum vulgare HVSMEn0010B09f Hordeum vulgare rachisEST 1 15 G157 BJ213269 3.00E−30 Triticum aestivum BJ213269 Y. Ogiharaunpublished cDNA libr 15 G157 BU887610 3.00E−30 Populus tremula xR064B01 Populus root Populus tremuloides 15 G157 gi17933450 4.90E−62Brassica napus MADS-box protein. 15 G157 gi9367313 2.60E−31 Hordeumvulgare MADS-box protein 8. 15 G157 gi16874557 5.50E−31 Antirrhinummajus MADS-box transcription factor DEFH28. 15 G157 gi1483232 7.00E−31Betula pendula MADS5 protein. 15 G157 gi4204234 1.40E−30 Loliumtemulentum MADS-box protein 2. 15 G157 gi7592642 1.40E−30 Oryza sativaAP1-like MADS box protein. 15 G157 gi12002141 1.80E−30 Zea mays MADS boxprotein 3. 15 G157 gi21070923 1.80E−30 Oryza sativa (japonica AP1-likeMADS-box cultivar-group) 15 G157 gi13384068 8.00E−30 Petunia x hybridaMADS-box transcription factor FBP29. 15 G157 gi6469345 1.30E−29 Brassicarapa subsp. DNA-binding protein. pekinensis 17 G162 BZ073323 6.00E−44Brassica oleracea lkf66e08.b1 B. oleracea002 Brassica olerac 17 G162BQ403135 3.00E−33 Gossypium arboreum GA_Ed0054C07f Gossypium arboreum7-10 d 17 G162 AC122160 2.00E−27 Medicago truncatula clone mth2-23d6,WORKING DRAFT SEQUENCE 17 G162 CRU91416 2.00E−18 Ceratopteris richardiiCMADS2 mRNA, complete cds. 17 G162 AP005789 3.00E−18 Oryza sativa(japonica ( ) chromosome 9 clo cultivar-group) 17 G162 AAAA010071383.00E−18 Oryza sativa (indica ( ) scaffold007138 cultivar-group) 17 G162AP003627 8.00E−18 Oryza sativa genomic DNA, chromosome 1, PAC clone:P0459B04, 17 G162 BZ415846 1.00E−16 Zea mays if60b04.g1 WGS-ZmaysF (DH5amethyl filtered) Zea m 17 G162 CA733624 3.00E−16 Triticum aestivumwlp1c.pk005.p15 wlp1c Triticum aestivum c 17 G162 AF035379 4.00E−16Lolium temulentum MADS-box protein 2 (MADS2) mRNA, alternat 17 G162gi3253149 1.30E−20 Ceratopteris richardii CMADS2. 17 G162 gi152901412.80E−20 Oryza sativa hypothetical protein. 17 G162 gi6580943 2.40E−19Picea abies MADS-box transcription factor. 17 G162 gi5019431 4.90E−19Gnetum gnemon putative MADS domain transcription factor G 17 G162gi1206005 4.90E−19 Pinus radiata putative MADS-box family transcriptionfact 17 G162 gi1702951 4.90E−19 Pinus resinosa MADS-box familytranscription factor. 17 G162 gi887392 8.00E−19 Brassica oleracea BOAP1.17 G162 gi21396799 1.60E−18 Lycopodium annotinum MADS-box gene 4protein. 17 G162 gi20219014 3.40E−18 Lycopersicon MADS-box transcriptionesculentum factor MAD 17 G162 gi7672991 3.60E−18 Canavalia lineataMADS-box transcription factor. 19 G175 AB063576 1.00E−108 Nicotianatabacum NtWRKY-9 mRNA for WRKY DNA-binding protei 19 G175 LES3033431.00E−103 Lycopersicon mRNA for hypothetical esculentum protein (ORF 19G175 BZ005522 2.00E−74 Brassica oleracea oej73d10.b1 B. oleracea002Brassica olerac 19 G175 IPBSPF1P 3.00E−71 Ipomoea batatas Sweet potatomRNA for SPF1 protein, complet 19 G175 AX192162 3.00E−68 Glycine maxSequence 9 from Patent WO0149840. 19 G175 AX192164 1.00E−66 Triticumaestivum Sequence 11 from Patent WO0149840. 19 G175 AF439274 5.00E−65Retama raetam WRKY-like drought- induced protein (WRK) mRNA, 19 G175OSJN00012 5.00E−64 Oryza sativa chromosome 4 clone OSJNBa0089K21, ***SEQUENC 19 G175 CUSSLDB 6.00E−63 Cucumis sativus SPF1-like DNA-bindingprotein mRNA, complet 19 G175 PCU48831 7.00E−63 Petroselinum crispumDNA-binding protein WRKY1 mRNA, comple 19 G175 gi13620227 8.20E−108Lycopersicon hypothetical protein. esculentum 19 G175 gi145306872.00E−89 Nicotiana tabacum WRKY DNA-binding protein. 19 G175 gi10766852.10E−74 Ipomoea batatas SPF1 protein-sweet potato. 19 G175 gi181586191.10E−69 Retama raetam WRKY-like drought- induced protein. 19 G175gi7484759 5.90E−68 Cucumis sativus SP8 binding protein homolog-cucumber.19 G175 gi5917653 7.80E−64 Petroselinum crispum zinc-finger typetranscription facto 19 G175 gi14587365 2.40E−63 Oryza sativa putativeDNA-binding protein ABF1. 19 G175 gi4894965 9.90E−61 Avena sativaDNA-binding protein WRKY1. 19 G175 gi1159877 2.40E−60 Avena fatuaDNA-binding protein. 19 G175 gi16588566 7.30E−52 Solanum dulcamarathermal hysteresis protein STHP-64. 21 G180 BU896559 7.00E−66 Populustremula x X042D08 Populus wood Populus tremuloides 21 G180 CA8002012.00E−58 Glycine max sat79d02.y1 Gm-c1062 Glycine max cDNA clone SOY 21G180 BQ507128 8.00E−55 Solanum tuberosum EST614543 Generation of a setof potato c 21 G180 BJ322852 1.00E−39 Triticum aestivum BJ322852 Y.Ogihara unpublished cDNA libr 21 G180 BQ293390 8.00E−39 Zea mays1091013C10.x2 1091- Immature ear with common ESTs 21 G180 BM3704409.00E−30 Hordeum vulgare EBro08_SQ004_D21_R IGF Barley EBro08 librar 21G180 AF140554 3.00E−28 Avena sativa DNA-binding protein WRKY1 (wrky1)mRNA, comple 21 G180 BI210061 1.00E−27 Lycopersicon EST528101 cTOSesculentum Lycopersicon esculen 21 G180 AFABF1 4.00E−27 Avena fatua A.fatua mRNA for DNA- binding protein (clone ABF 21 G180 BQ864325 2.00E−26Lactuca sativa QGC26J22.yg.ab1 QG_ABCDI lettuce salinas Lac 21 G180gi14140117 9.60E−50 Oryza sativa WRKY-like DNA-binding protein. 21 G180gi24745606 1.10E−31 Solanum tuberosum WRKY-type DNA binding protein. 21G180 gi4894965 1.90E−29 Avena sativa DNA-binding protein WRKY1. 21 G180gi1159877 3.50E−29 Avena fatua DNA-binding protein. 21 G180 gi201610045.60E−29 Oryza sativa (japonica hypothetical prote cultivar-group) 21G180 gi1431872 7.30E−29 Petroselinum crispum WRKY1. 21 G180 gi53606836.90E−28 Nicotiana tabacum NtWRKY1. 21 G180 gi13620227 3.50E−27Lycopersicon hypothetical protein. esculentum 21 G180 gi3420906 5.30E−27Pimpinella brachycarpa zinc finger protein; WRKY1. 21 G180 gi10766851.20E−26 Ipomoea batatas SPF1 protein-sweet potato. 23 G183 CRU3033493.00E−54 Capsella rubella ORF1, ORF2, ORF3, ORF4, ORF5 and ORF6 (pa 23G183 AB063576 5.00E−33 Nicotiana tabacum NtWRKY-9 mRNA for WRKYDNA-binding protei 23 G183 LES303343 3.00E−32 Lycopersicon mRNA forhypothetical esculentum protein (ORF 23 G183 IPBSPF1P 2.00E−29 Ipomoeabatatas Sweet potato mRNA for SPF1 protein, complet 23 G183 BM4082052.00E−29 Solanum tuberosum EST582532 potato roots Solanum tuberosum 23G183 BI128063 5.00E−29 Populus tremula x G070P32Y Populus camb Populustremuloides 23 G183 BU043758 1.00E−28 Prunus persica PP_LEa0017B09fPeach developing fruit mesoca 23 G183 AX192162 4.00E−28 Glycine maxSequence 9 from Patent WO0149840. 23 G183 BG442954 5.00E−28 Gossypiumarboreum GA_Ea0018P14f Gossypium arboreum 7-10 d 23 G183 AF0805952.00E−27 Pimpinella brachycarpa zinc finger protein (ZFP1) mRNA, com 23G183 gi13620168 1.30E−86 Capsella rubella hypothetical protein. 23 G183gi13620227 2.60E−52 Lycopersicon hypothetical protein. esculentum 23G183 gi6174838 1.10E−37 Nicotiana tabacum transcription factor NtWRKY4.23 G183 gi1076685 1.70E−35 Ipomoea batatas SPF1 protein-sweet potato. 23G183 gi7484759 9.20E−29 Cucumis sativus SP8 binding proteinhomolog-cucumber. 23 G183 gi1159877 9.50E−29 Avena fatua DNA-bindingprotein. 23 G183 gi14587365 8.00E−28 Oryza sativa putative DNA-bindingprotein ABF1. 23 G183 gi3420906 1.10E−27 Pimpinella brachycarpa zincfinger protein; WRKY1. 23 G183 gi5917653 1.00E−26 Petroselinum crispumzinc-finger type transcription facto 23 G183 gi4894965 2.30E−26 Avenasativa DNA-binding protein WRKY1. 25 G188 AW596933 6.00E−43 Glycine maxsj84f07.y1 Gm-c1034 Glycine max cDNA clone GENO 25 G188 BI9234142.00E−40 Lycopersicon EST543319 tomato callus esculentum Lycopersico 25G188 AV423663 3.00E−40 Lotus japonicus AV423663 Lotus japonicus youngplants (two- 25 G188 BM112869 6.00E−39 Solanum tuberosum EST560405potato roots Solanum tuberosum 25 G188 AP003951 6.00E−39 Oryza sativachromosome 6 clone OJ1288_C01, *** SEQUENCING 25 G188 AP004683 9.00E−39Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 25 G188AAAA01011017 9.00E−39 Oryza sativa (indica ( ) scaffold011017cultivar-group) 25 G188 BU837263 6.00E−38 Populus tremula x T096G05Populus apica Populus tremuloides 25 G188 AW447931 4.00E−34 Triticumaestivum BRY_1082 BRY Triticum aestivum cDNA clone 25 G188 BQ7639962.00E−32 Hordeum vulgare subsp. EBro03_SQ006_A04_R vulgare root, 3 w 25G188 gi12039364 4.00E−37 Oryza sativa putative DNA-binding protein. 25G188 gi4322940 4.70E−21 Nicotiana tabacum DNA-binding protein 2. 25 G188gi4894963 5.00E−20 Avena sativa DNA-binding protein WRKY3. 25 G188gi1432056 7.80E−20 Petroselinum crispum WRKY3. 25 G188 gi119939013.10E−18 Dactylis glomerata somatic embryogenesis related protein. 25G188 gi22830985 1.10E−17 Oryza sativa (japonica WRKY transcriptioncultivar-group) 25 G188 gi7484759 1.40E−16 Cucumis sativus SP8 bindingprotein homolog-cucumber. 25 G188 gi1159879 2.70E−15 Avena fatuaDNA-binding protein. 25 G188 gi23305051 8.00E−15 Oryza sativa (indicaWRKY transcription f cultivar-group) 25 G188 gi9187622 2.70E−14 Solanumtuberosum WRKY DNA binding protein. 27 G189 AB041520 2.00E−67 Nicotianatabacum mRNA for WRKY transcription factor Nt-Sub 27 G189 PCU568342.00E−64 Petroselinum crispum DNA binding protein WRKY3 mRNA, comple 27G189 AF140553 6.00E−55 Avena sativa DNA-binding protein WRKY3 (wrky3)mRNA, comple 27 G189 BI469529 1.00E−54 Glycine max sah61a11.y1 Gm-c1049Glycine max cDNA clone GEN 27 G189 AY108689 5.00E−54 Zea mays PCO134907mRNA sequence. 27 G189 AAAA01014145 7.00E−54 Oryza sativa (indica ( )scaffold014145 cultivar-group) 27 G189 BI209749 2.00E−53 LycopersiconEST527789 cTOS esculentum Lycopersicon esculen 27 G189 BU046845 4.00E−53Prunus persica PP_LEa0027O15f Peach developing fruit mesoca 27 G189AP004648 4.00E−51 Oryza sativa (japonica ( ) chromosome 8 clocultivar-group) 27 G189 OSJN00198 6.00E−48 Oryza sativa chromosome 4clone OSJNBb0015N08, *** SEQUENC 27 G189 gi4894963 1.00E−54 Avena sativaDNA-binding protein WRKY3. 27 G189 gi10798760 1.70E−50 Nicotiana tabacumWRKY transcription factor Nt-SubD48. 27 G189 gi1432056 1.60E−49Petroselinum crispum WRKY3. 27 G189 gi11993901 5.80E−43 Dactylisglomerata somatic embryogenesis related protein. 27 G189 gi152898295.60E−25 Oryza sativa contains ESTs D24303(R1701), C26098(C11628) ~u 27G189 gi1076685 1.60E−21 Ipomoea batatas SPF1 protein-sweet potato. 27G189 gi1159877 6.50E−21 Avena fatua DNA-binding protein. 27 G189gi18158619 5.10E−20 Retama raetam WRKY-like drought- induced protein. 27G189 gi3420906 9.80E−20 Pimpinella brachycarpa zinc finger protein;WRKY1. 27 G189 gi23305051 4.50E−19 Oryza sativa (indica WRKYtranscription f cultivar-group) 29 G192 BH471182 3.00E−62 Brassicaoleracea BOHES67TF BOHE Brassica oleracea genomic 29 G192 BI9232352.00E−49 Lycopersicon EST543139 tomato callus esculentum Lycopersico 29G192 AW596933 3.00E−47 Glycine max sj84f07.y1 Gm-c1034 Glycine max cDNAclone GENO 29 G192 AV423663 2.00E−46 Lotus japonicus AV423663 Lotusjaponicus young plants (two- 29 G192 BM112869 1.00E−41 Solanum tuberosumEST560405 potato roots Solanum tuberosum 29 G192 BU837263 8.00E−39Populus tremula x T096G05 Populus apica Populus tremuloides 29 G192AAAA01003718 6.00E−34 Oryza sativa (indica ( ) scaffold003718cultivar-group) 29 G192 AC018727 6.00E−34 Oryza sativa chromosome 10clone OSJNBa0056G17, *** SEQUENC 29 G192 AP004683 1.00E−33 Oryza sativa(japonica ( ) chromosome 2 clo cultivar-group) 29 G192 AW447931 1.00E−32Triticum aestivum BRY_1082 BRY Triticum aestivum cDNA clone 29 G192gi12039364 1.90E−35 Oryza sativa putative DNA-binding protein. 29 G192gi1432056 2.00E−24 Petroselinum crispum WRKY3. 29 G192 gi48949638.80E−24 Avena sativa DNA-binding protein WRKY3. 29 G192 gi47605961.80E−23 Nicotiana tabacum DNA-binding protein NtWRKY3. 29 G192gi11993901 4.30E−20 Dactylis glomerata somatic embryogenesis relatedprotein. 29 G192 gi21644680 1.60E−17 Oryza sativa (japonica hypotheticalprote cultivar-group) 29 G192 gi23305051 5.00E−17 Oryza sativa (indicaWRKY transcription f cultivar-group) 29 G192 gi1076685 1.90E−15 Ipomoeabatatas SPF1 protein-sweet potato. 29 G192 gi7484759 2.30E−15 Cucumissativus SP8 binding protein homolog-cucumber. 29 G192 gi3420906 5.10E−15Pimpinella brachycarpa zinc finger protein; WRKY1. 31 G196 BH9449619.00E−69 Brassica oleracea obu81g06.g1 B. oleracea002 Brassica olerac 31G196 AAAA01003718 1.00E−46 Oryza sativa (indica ( ) scaffold003718cultivar-group) 31 G196 AC018727 1.00E−46 Oryza sativa chromosome 10clone OSJNBa0056G17, *** SEQUENC 31 G196 BI923235 6.00E−40 LycopersiconEST543139 tomato callus esculentum Lycopersico 31 G196 BM113882 4.00E−38Solanum tuberosum EST561418 potato roots Solanum tuberosum 31 G196AW596933 1.00E−35 Glycine max sj84f07.y1 Gm-c1034 Glycine max cDNA cloneGENO 31 G196 AV423663 2.00E−34 Lotus japonicus AV423663 Lotus japonicusyoung plants (two- 31 G196 BG647709 3.00E−34 Medicago truncatulaEST509328 HOGA Medicago truncatula cDNA 31 G196 BQ855766 3.00E−33Lactuca sativa QGB27K18.yg.ab1 QG_ABCDI lettuce salinas Lac 31 G196BU837263 5.00E−32 Populus tremula x T096G05 Populus apica Populustremuloides 31 G196 gi12039364 3.30E−51 Oryza sativa putativeDNA-binding protein. 31 G196 gi4894963 2.40E−27 Avena sativa DNA-bindingprotein WRKY3. 31 G196 gi10798760 7.00E−26 Nicotiana tabacum WRKYtranscription factor Nt-SubD48. 31 G196 gi1432056 6.20E−25 Petroselinumcrispum WRKY3. 31 G196 gi11993901 3.00E−20 Dactylis glomerata somaticembryogenesis related protein. 31 G196 gi20160973 3.50E−20 Oryza sativa(japonica hypothetical prote cultivar-group) 31 G196 gi23305051 1.10E−14Oryza sativa (indica WRKY transcription f cultivar-group) 31 G196gi9187622 1.40E−14 Solanum tuberosum WRKY DNA binding protein. 31 G196gi1076685 2.50E−14 Ipomoea batatas SPF1 protein-sweet potato. 31 G196gi13620227 5.50E−14 Lycopersicon hypothetical protein. esculentum 33G211 BG441912 6.00E−70 Gossypium arboreum GA_Ea0015B19f Gossypiumarboreum 7-10 d 33 G211 AF336278 1.00E−69 Gossypium hirsutum BNLGHi233(bnlghi6233) mRNA, complete cd 33 G211 BU837990 3.00E−66 Populus tremulax T108C04 Populus apica Populus tremuloides 33 G211 D88620 2.00E−57Oryza sativa mRNA for OSMYB4, complete cds. 33 G211 AW186273 6.00E−54Glycine max se65f12.y1 Gm-c1019 Glycine max cDNA clone GENO 33 G211PMU39448 1.00E−52 Picea mariana MYB-like transcriptional factor MBF1mRNA, co 33 G211 AAAA01005841 1.00E−52 Oryza sativa (indica ( )scaffold005841 cultivar-group) 33 G211 BI674748 7.00E−52 Zea mays949066G11.y2 949- Juvenile leaf and shoot cDNA fr 33 G211 AW7758932.00E−51 Medicago truncatula EST334958 DSIL Medicago truncatula cDNA 33G211 HVMYB2 2.00E−51 Hordeum vulgare H. vulgare myb2 mRNA. 33 G211gi13346178 1.50E−67 Gossypium hirsutum BNLGHi233. 33 G211 gi225355561.10E−53 Oryza sativa (japonica myb-related protei cultivar-group) 33G211 gi2605623 1.10E−53 Oryza sativa OSMYB4. 33 G211 gi1101770 5.70E−52Picea mariana MYB-like transcriptional factor MBF1. 33 G211 gi823102.00E−51 Antirrhinum majus myb protein 330-garden snapdragon. 33 G211gi127582 4.00E−51 Zea mays MYB-RELATED PROTEIN ZM38. 33 G211 gi190551.10E−50 Hordeum vulgare MybHv5. 33 G211 gi22795039 1.10E−50 Populus xcanescens putative MYB transcription factor. 33 G211 gi1167484 3.60E−50Lycopersicon transcription factor. esculentum 33 G211 gi20563 3.70E−50Petunia x hybrida protein 1. 35 G214 PVU420902 1.00E−111 Phaseolusvulgaris mRNA for LHY protein. 35 G214 BU868664 6.00E−60 Populusbalsamifera M118F07 Populus flow subsp. trichocarpa 35 G214 BE3315632.00E−50 Glycine max sp15d08.y1 Gm-c1042 Glycine max cDNA clone GENO 35G214 BH935194 1.00E−49 Brassica oleracea ode18e05.g1 B. oleracea002Brassica olerac 35 G214 AAAA01009649 4.00E−49 Oryza sativa (indica ( )scaffold009649 cultivar-group) 35 G214 AP004460 5.00E−48 Oryza sativa(japonica ( ) chromosome 8 clo cultivar-group) 35 G214 AW979367 2.00E−46Lycopersicon EST310415 tomato root esculentum deficiency, C 35 G214BM322287 5.00E−46 Sorghum bicolor PIC1_2_F02.b1_A002 Pathogen-infectedcompat 35 G214 AY103618 4.00E−45 Zea mays PCO118792 mRNA sequence. 35G214 BG524104 3.00E−44 Stevia rebaudiana 38-82 Stevia field grown leafcDNA Stevia 35 G214 gi21213868 7.60E−57 Phaseolus vulgaris LHY protein.35 G214 gi15528628 2.40E−23 Oryza sativa hypothetical protein~similar toOryza sativa 35 G214 gi12406993 1.20E−06 Hordeum vulgare MCB1 protein.35 G214 gi20067661 1.40E−06 Zea mays one repeat myb transcriptionalfactor. 35 G214 gi18874263 3.70E−06 Antirrhinum majus MYB-liketranscription factor DIVARICAT 35 G214 gi24850305 1.00E−05 Oryza sativa(japonica transcription fact cultivar-group) 35 G214 gi12005328 3.00E−05Hevea brasiliensis unknown. 35 G214 gi6688529 6.80E−05 LycopersiconI-box binding factor. esculentum 35 G214 gi19911579 7.10E−05 Glycine maxsyringolide-induced protein 1-3-1B. 35 G214 gi7677132 0.0025 Secalecereale c-myb-like transcription factor. 37 G226 BU872107 2.00E−21Populus balsamifera Q039C07 Populus flow subsp. trichocarpa 37 G226BU831849 2.00E−21 Populus tremula x T026E01 Populus apica Populustremuloides 37 G226 BM437313 9.00E−21 Vitis vinifera VVA017F06_54121 Anexpressed sequence tag da 37 G226 BI699876 1.00E−19 Glycine maxsag49b09.y1 Gm-c1081 Glycine max cDNA clone GEN 37 G226 AL7501514.00E−16 Pinus pinaster AL750151 AS Pinus pinaster cDNA clone AS06C1 37G226 CA744013 2.00E−12 Triticum aestivum wri1s.pk006.m22 wri1s Triticumaestivum c 37 G226 BH961028 3.00E−12 Brassica oleracea odj30d06.g1 B.oleracea002 Brassica olerac 37 G226 BJ472717 8.00E−12 Hordeum vulgaresubsp. BJ472717 K. Sato vulgare unpublished 37 G226 BF617445 8.00E−12Hordeum vulgare HVSMEc0017G08f Hordeum vulgare seedling sho 37 G226CA762299 2.00E−11 Oryza sativa (indica BR060003B10F03.ab1 IRRcultivar-group) 37 G226 gi9954118 2.20E−11 Solanum tuberosumtuber-specific and sucrose- responsive e 37 G226 gi14269333 2.50E−10Gossypium raimondii myb-like transcription factor Myb 3. 37 G226gi14269335 2.50E−10 Gossypium herbaceum myb-like transcription factorMyb 3. 37 G226 gi14269337 2.50E−10 Gossypium hirsutum myb-liketranscription factor Myb 3. 37 G226 gi23476297 2.50E−10 Gossypioideskirkii myb-like transcription factor 3. 37 G226 gi15082210 8.50E−10Fragaria x ananassa transcription factor MYB1. 37 G226 gi190727708.50E−10 Oryza sativa typical P-type R2R3 Myb protein. 37 G226gi15042108 1.40E−09 Zea mays subsp. CI protein. parviglumis 37 G226gi15042124 1.40E−09 Zea luxurians CI protein. 37 G226 gi205143711.40E−09 Cucumis sativus werewolf. 39 G241 AB028650 3.00E−69 Nicotianatabacum mRNA for myb-related transcription factor 39 G241 PHMYBPH223.00E−68 Petunia x hybrida P. Hybrida myb.Ph2 gene encoding protein 39G241 LETHM18GE 1.00E−65 Lycopersicon L. esculentum mRNA for esculentummyb-related 39 G241 AB073017 2.00E−63 Vitis labrusca x Vitis VlmybB1-2gene for myb- vinifera rela 39 G241 OSMYB1202 5.00E−63 Oryza sativa O.sativa mRNA for myb factor, 1202 bp. 39 G241 AB029162 2.00E−62 Glycinemax gene for GmMYB293, complete cds. 39 G241 BQ514539 1.00E−61 Solanumtuberosum EST621954 Generation of a set of potato c 39 G241 AW9811675.00E−61 Medicago truncatula EST392361 DSIL Medicago truncatula cDNA 39G241 BJ312394 4.00E−60 Triticum aestivum BJ312394 Y. Ogihara unpublishedcDNA libr 39 G241 BM816803 2.00E−59 Hordeum vulgare HC114B11_SK.ab1 HCHordeum vulgare cDNA clo 39 G241 gi6552361 1.50E−67 Nicotiana tabacummyb-related transcription factor LBM2. 39 G241 gi20561 8.30E−67 Petuniax hybrida protein 2. 39 G241 gi1370140 3.70E−64 Lycopersicon myb-relatedtranscription esculentum factor. 39 G241 gi6492385 3.80E−62 Glycine maxGmMYB29A2. 39 G241 gi1946265 2.70E−61 Oryza sativa myb. 39 G241gi22266675 9.70E−57 Vitis labrusca x Vitis myb-related transcriptionvinifera 39 G241 gi127580 5.50E−54 Zea mays MYB-RELATED PROTEIN ZM1. 39G241 gi11526779 9.90E−52 Zea mays subsp. P-like protein. parviglumis 39G241 gi22795039 1.10E−48 Populus x canescens putative MYB transcriptionfactor. 39 G241 gi13346188 1.40E−48 Gossypium hirsutum GHMYB25. 41 G248BE642935 2.00E−25 Ceratopteris richardii Cri2_7_G20_SP6 CeratopterisSpore Li 41 G248 AF190304 1.00E−24 Adiantum raddianum c-myb-liketranscription factor (MYB3R-1 41 G248 AW040511 1.00E−24 LycopersiconEST283471 tomato mixed esculentum elicitor, BT 41 G248 AF189786 2.00E−24Physcomitrella patens putative c-myb-like transcription fac 41 G248CA755789 4.00E−24 Oryza sativa (japonica BR030028000_PLATE_D1cultivar-group) 41 G248 AB056123 2.00E−23 Nicotiana tabacum NtmybA2 mRNAfor Myb, complete cds. 41 G248 AF189788 2.00E−22 Hordeum vulgareputative c-myb-like transcription factor (M 41 G248 AF236059 3.00E−22Papaver rhoeas putative Myb-related domain (pmr) mRNA, part 41 G248AF190302 2.00E−20 Secale cereale c-myb-like transcription factor(MYB3R-1) mR 41 G248 BH444284 1.00E−18 Brassica oleracea BOGON79TF BOGOBrassica oleracea genomic 41 G248 gi24417180 6.50E−28 Oryza sativa(japonica myb-like protein. cultivar-group) 41 G248 gi7677136 5.80E−27Adiantum raddianum c-myb-like transcription factor. 41 G248 gi87453257.30E−25 Hordeum vulgare putative c-myb-like transcription factor. 41G248 gi8745321 2.30E−24 Physcomitrella patens putative c-myb-liketranscription f 41 G248 gi16326135 9.40E−23 Nicotiana tabacum Myb. 41G248 gi7677132 1.50E−22 Secale cereale c-myb-like transcription factor.41 G248 gi7630236 2.30E−22 Oryza sativa Similar to Arabidopsis thalianachromosome 4 41 G248 gi7230673 7.10E−22 Papaver rhoeas putativeMyb-related domain. 41 G248 gi14269337 1.50E−20 Gossypium hirsutummyb-like transcription factor Myb 3. 41 G248 gi14269333 1.60E−19Gossypium raimondii myb-like transcription factor Myb 3. 43 G254BU100118 4.00E−67 Triticum aestivum WHE3315_D06_H11ZS Chinese Springwheat dr 43 G254 BI921951 1.00E−60 Lycopersicon EST541854 tomato callusesculentum Lycopersico 43 G254 AV909036 1.00E−57 Hordeum vulgare subsp.AV909036 K. Sato vulgare unpublished 43 G254 AW000459 9.00E−54 Zea mays614016D07.y1 614-root cDNA library from Walbot L 43 G254 BG4577022.00E−53 Medicago truncatula NF034C07PL1F1051 Phosphate starved leaf 43G254 BU025460 2.00E−53 Helianthus annuus QHF9I05.yg.ab1 QH_EFGHJsunflower RHA280 43 G254 BG593097 3.00E−52 Solanum tuberosum EST491775cSTS Solanum tuberosum cDNA clo 43 G254 BU868480 3.00E−52 Populusbalsamifera M116D03 Populus flow subsp. trichocarpa 43 G254 BU8159735.00E−52 Populus tremula x N058E04 Populus bark Populus tremuloides 43G254 BE330818 1.00E−51 Glycine max so85g03.y1 Gm-c1041 Glycine max cDNAclone GENO 43 G254 gi15528628 1.80E−25 Oryza sativa hypotheticalprotein~similar to Oryza sativa 43 G254 gi21213868 3.40E−24 Phaseolusvulgaris LHY protein. 43 G254 gi18461206 1.20E−07 Oryza sativa (japonicacontains ESTs AU10 cultivar-group) 43 G254 gi12005328 1.10E−06 Heveabrasiliensis unknown. 43 G254 gi12406993 1.30E−06 Hordeum vulgare MCB1protein. 43 G254 gi19911577 5.50E−06 Glycine max syringolide-inducedprotein 1-3-1A. 43 G254 gi6688529 3.90E−05 Lycopersicon I-box bindingfactor. esculentum 43 G254 gi18874265 3.90E−05 Antirrhinum majusMYB-like transcription factor DVL1. 43 G254 gi20067661 4.10E−05 Zea maysone repeat myb transcriptional factor. 43 G254 gi7705206 0.00072 Solanumtuberosum MybSt1. 45 G256 LETHM6 1.00E−78 Lycopersicon L. esculentummRNA for esculentum myb-related t 45 G256 AY107969 4.00E−78 Zea maysPCO069276 mRNA sequence. 45 G256 BF270109 3.00E−76 Gossypium arboreumGA_Eb0006M14f Gossypium arboreum 7-10 d 45 G256 AW981415 5.00E−75Medicago truncatula EST392568 DSIL Medicago truncatula cDNA 45 G256BE342909 1.00E−72 Solanum tuberosum EST395753 potato stolon, CornellUniversi 45 G256 BQ623221 5.00E−72 Citrus sinensis USDA-FP_00312 Ridgepineapple sweet orange 45 G256 AP005636 1.00E−70 Oryza sativa (japonica( ) chromosome 9 clo cultivar-group) 45 G256 AAAA01005623 1.00E−70 Oryzasativa (indica ( ) scaffold005623 cultivar-group) 454 G256 AC0847628.00E−70 Oryza sativa chromosome 3 clone OSJNBa0013O08, *** SEQUENCI 45G256 BM309647 8.00E−67 Glycine max sak65a08.y1 Gm-c1036 Glycine max cDNAclone SOY 45 G256 gi256828 1.10E−80 Antirrhinum majus Myb oncoproteinhomolog {clone 306} [An 45 G256 gi1430848 8.20E−76 Lycopersicontranscription factor. esculentum 45 G256 gi18071376 6.80E−71 Oryzasativa putative transcription factor. 45 G256 gi23616974 3.60E−66 Oryzasativa (japonica contains EST C2815 cultivar-group) 45 G256 gi190727444.20E−65 Zea mays typical P-type R2R3 Myb protein. 45 G256 gi205637.30E−52 Petunia x hybrida protein 1. 45 G256 gi6552361 2.90E−50Nicotiana tabacum myb-related transcription factor LBM2. 45 G256gi13346188 2.30E−48 Gossypium hirsutum GHMYB25. 45 G256 gi51398024.70E−48 Glycine max GmMYB29A1. 45 G256 gi11526775 1.60E−47 Zea mayssubsp. P2-t protein. parviglumis 47 G278 AF527176  1.0e−999 Brassicanapus putative NPR1 (NPR1) mRNA, complete cds. 47 G278 BD064079 1.0e−999 Macadamia integrifolia Method for protecting plants. 47 G278AF480488 1.00E−162 Nicotiana tabacum NPR1 mRNA, complete cds. 47 G278AX351141 1.00E−106 Oryza sativa Sequence 15 from Patent WO0166755. 47G278 AX041006 8.00E−97 Zea mays Sequence 1 from Patent WO0065037. 47G278 AX351145 3.00E−95 Triticum aestivum Sequence 19 from PatentWO0166755. 47 G278 AC124609 2.00E−75 Medicago truncatula clonemth2-29b13, WORKING DRAFT SEQUENC 47 G278 AAAA01004121 6.00E−70 Oryzasativa (indica ( ) scaffold004121 cultivar-group) 47 G278 BZ0567115.00E−67 Brassica oleracea lle49h07.b1 B. oleracea002 Brassica olerac 47G278 BE435499 3.00E−50 Lycopersicon EST406577 tomato breaker esculentumfruit, TIG 47 G278 gi22003730 0.00E+00 Brassica napus putative NPR1. 47G278 gi21552981 9.30E−155 Nicotiana tabacum NPR1. 47 G278 gi109340821.40E−128 Oryza sativa Arabidopsis thaliana regulatory protein NPR1 47G278 gi18616499 5.00E−92 Triticum aestivum unnamed protein product. 47G278 gi22535593 2.60E−88 Oryza sativa (japonica putative Regulatorcultivar-group) 47 G278 gi11340603 3.40E−86 Zea mays unnamed proteinproduct. 47 G278 gi17645766 0.00027 Glycine max unnamed protein product.47 G278 gi549986 0.012 Pennisetum ciliare possible apospory- associatedprotein. 47 G278 gi18700703 0.14 Medicago sativa putativeankyrin-kinase. 47 G278 gi18700701 0.18 Medicago truncatulaankyrin-kinase. 49 G291 AF014375 1.00E−170 Medicago sativa putative JUNkinase activation domain bindi 49 G291 AF175964 1.00E−169 LycopersiconJAB mRNA, complete cds. esculentum 49 G291 AF072849 1.00E−159 Oryzasativa subsp. jab1 protein (jab1) mRNA, indica comple 49 G291 AB0554951.00E−159 Oryza sativa Jab1 mRNA for JUN- activation-domain-binding pr49 G291 BG594615 1.00E−132 Solanum tuberosum EST493293 cSTS Solanumtuberosum cDNA clo 49 G291 BQ969736 1.00E−125 Helianthus annuusQHB39G11.yg.ab1 QH_ABCDI sunflower RHA801 49 G291 BQ871378 1.00E−123Lactuca sativa QGI11K21.yg.ab1 QG_ABCDI lettuce salinas Lac 49 G291BE036313 1.00E−115 Mesembryanthemum MO23B10 MO crystallinumMesembryanthemum c 49 G291 BM066924 1.00E−113 Capsicum annuum KS07019G04KS07 Capsicum annuum cDNA, mRNA 49 G291 BQ281547 1.00E−106 Triticumaestivum WHE3022_F07_K14ZS Wheat unstressed seedli 49 G291 gi33203791.80E−160 Medicago sativa putative JUN kinase activation domain bin 49G291 gi12002865 3.00E−158 Lycopersicon JAB. esculentum 49 G291gi17025926 4.30E−150 Oryza sativa JUN-activation-domain- binding proteinhomolo 49 G291 gi24636586 4.30E−150 Oryza sativa (japonicaJUN-activation-dom cultivar-group) 49 G291 gi3420299 4.30E−150 Oryzasativa subsp. jab1 protein. indica 49 G291 gi13774977 0.73 Pinus mugoNADH dehydrogenase subunit 3. 49 G291 gi13774980 0.73 Pinus sylvestrisNADH dehydrogenase subunit 3. 49 G291 gi13899006 0.89 Abies alba NADHdehydrogenase subunit 3. 49 G291 gi23503480 1 Glycine max heat shockprotein DnaJ. 51 G303 BI677665 2.00E−40 Robinia pseudoacacia CLS342 CLS(Cambium and bark region of 51 G303 BQ995023 2.00E−38 Lactuca sativaQGF8N12.yg.ab1 QG_EFGHJ lettuce serriola Lac 51 G303 AAAA010033455.00E−36 Oryza sativa (indica ( ) scaffold003345 cultivar-group) 51 G303AC121489 6.00E−36 Oryza sativa (japonica ( ) chromosome 3 clocultivar-group) 51 G303 BE022329 6.00E−35 Glycine max sm73e05.y1Gm-c1028 Glycine max cDNA clone GENO 51 G303 BI480474 2.00E−32 Triticumaestivum WHE2903_F02_L03ZS Wheat aluminum-stressed 51 G303 BH4922557.00E−32 Brassica oleracea BOHLS25TR BOHL Brassica oleracea genomic 51G303 BI128898 2.00E−30 Populus tremula x G083P21Y Populus camb Populustremuloides 51 G303 CAR011013 1.00E−29 Cicer arietinum epicotyl EST,clone Can133. 51 G303 AW573949 4.00E−27 Medicago truncatula EST316540GVN Medicago truncatula cDNA 51 G303 gi19920107 4.50E−43 Oryza sativa(japonica Putative helix-loo cultivar-group) 51 G303 gi3641870 4.30E−31Cicer arietinum hypothetical protein. 51 G303 gi10998404 1.90E−09Petunia x hybrida anthocyanin 1. 51 G303 gi18568238 2.10E−08 Zea maysregulatory protein. 51 G303 gi527661 2.90E−08 Phyllostachys acutamyc-like regulatory R gene product. 51 G303 gi1086538 6.10E−08 Oryzarufipogon transcriptional activator Rb homolog. 51 G303 gi5276536.10E−08 Pennisetum glaucum myc-like regulatory R gene product. 51 G303gi1086534 7.90E−08 Oryza officinalis transcriptional activator Rahomolog. 51 G303 gi1086540 1.90E−07 Oryza sativa Ra. 51 G303 gi5276634.70E−07 Tripsacum australe myc-like regulatory R gene product. 53 G312AAAA01008118 1.00E−137 Oryza sativa (indica ( ) scaffold008118cultivar-group) 53 G312 BH521755 1.00E−69 Brassica oleracea BOHEY85TFBOHE Brassica oleracea genomic 53 G312 AW944694 4.00E−67 Euphorbia esula00182 leafy spurge Lambda HybriZAP 2.1 two- 53 G312 BQ296629 3.00E−66Glycine max san83a05.y2 Gm-c1052 Glycine max cDNA clone SOY 53 G312BG446635 7.00E−64 Gossypium arboreum GA_Eb0036G15f Gossypium arboreum7-10 d 53 G312 BH873477 8.00E−60 Zea mays hp45c06.b2 WGS-ZmaysF (JM107adapted methyl filter 53 G312 BF257184 4.00E−56 Hordeum vulgareHVSMEf0012B22f Hordeum vulgare seedling roo 53 G312 AV414014 1.00E−52Lotus japonicus AV414014 Lotus japonicus young plants (two- 53 G312AF098674 4.00E−52 Lycopersicon lateral suppressor protein esculentum(Ls) mRN 53 G312 AB048713 2.00E−51 Pisum sativum PsSCR mRNA forSCARECROW, complete cds. 53 G312 gi13365610 1.30E−57 Pisum sativumSCARECROW. 53 G312 gi10178637 2.60E−53 Zea mays SCARECROW. 53 G312gi13620224 1.30E−52 Lycopersicon lateral suppressor. esculentum 53 G312gi13937306 4.80E−50 Oryza sativa gibberellin-insensitive protein OsGAI.53 G312 gi20334379 1.80E−48 Vitis vinifera GAI-like protein 1. 53 G312gi19571020 5.80E−48 Oryza sativa (japonica contains ESTs AU16cultivar-group) 53 G312 gi13620166 4.20E−47 Capsella rubellahypothetical protein. 53 G312 gi13170126 1.30E−45 Brassica napus unnamedprotein product. 53 G312 gi20257438 7.60E−44 Argyroxiphium GIA/RGA-lisandwicense subsp. macrocephalum 53 G312 gi20257420 9.60E−44 Dubautiaarborea GIA/RGA-like gibberellin response modula 55 G325 AB0018886.00E−41 Oryza sativa mRNA for zinc finger protein, complete cds, 55G325 AAAA01003074 3.00E−32 Oryza sativa (indica ( ) scaffold003074cultivar-group) 55 G325 BQ458955 2.00E−31 Hordeum vulgare HA02L20r HAHordeum vulgare cDNA clone HA02 55 G325 AP005113 3.00E−31 Oryza sativa(japonica ( ) chromosome 2 clo cultivar-group) 55 G325 BJ209915 6.00E−31Triticum aestivum BJ209915 Y. Ogihara unpublished cDNA libr 55 G325BG644908 2.00E−30 Medicago truncatula EST506527 KV3 Medicago truncatulacDNA 55 G325 BG459023 2.00E−29 Zea mays 947052H08.y1 947-2 week shootfrom Barkan lab Ze 55 G325 BQ121038 4.00E−29 Solanum tuberosum EST606614mixed potato tissues Solanum tu 55 G325 AP004972 4.00E−29 Lotusjaponicus genomic DNA, chromosome 3, clone: LjT41A07, 55 G325 BH9265191.00E−28 Brassica oleracea odj42f08.b1 B. oleracea002 Brassica olerac 55G325 gi3618320 9.80E−48 Oryza sativa zinc finger protein. 55 G325gi3341723 1.70E−15 Raphanus sativus CONSTANS-like 1 protein. 55 G325gi22854952 2.20E−15 Brassica nigra COL1 protein. 55 G325 gi23036832.00E−14 Brassica napus unnamed protein product. 55 G325 gi234958712.30E−13 Oryza sativa (japonica putative zinc-fing cultivar-group) 55G325 gi4091806 3.80E−13 Malus x domestica CONSTANS-like protein 2. 55G325 gi10946337 6.20E−13 Ipomoea nil CONSTANS-like protein. 55 G325gi21667475 2.00E−11 Hordeum vulgare CONSTANS-like protein. 55 G325gi4557093 1.10E−10 Pinus radiata zinc finger protein. 55 G325 gi216551541.20E−09 Hordeum vulgare subsp. CONSTANS-like protein vulgare CO5. 57G343 AC069300 2.00E−50 Oryza sativa chromosome 10 clone OSJNBa0010C11,*** SEQUENC 57 G343 BU827056 4.00E−50 Populus tremula x UK127TH09Populus api Populus tremuloides 57 G343 AAAA01001158 1.00E−47 Oryzasativa (indica ( ) scaffold001158 cultivar-group) 57 G343 BQ4626443.00E−41 Hordeum vulgare HI01J05T HI Hordeum vulgare cDNA clone HI01 57G343 AW235021 4.00E−41 Glycine max sf21h11.y1 Gm-c1028 Glycine max cDNAclone GENO 57 G343 BZ328210 8.00E−41 Zea mays id36b06.g1 WGS-ZmaysF(JM107 adapted methyl filter 57 G343 BH534811 1.00E−40 Brassica oleraceaBOGJZ23TF BOGJ Brassica oleracea genomic 57 G343 BQ851743 2.00E−37Lactuca sativa QGB16C22.yg.ab1 QG_ABCDI lettuce salinas Lac 57 G343AW922818 5.00E−37 Sorghum bicolor DG1_46_F02.g1_A002 Dark Grown 1 (DG1)Sorgh 57 G343 AC132491 9.00E−37 Oryza sativa (japonica ( ) chromosome 5clo cultivar-group) 57 G343 gi14165317 2.10E−57 Oryza sativa putativetranscription factor. 57 G343 gi21902044 6.50E−45 Oryza sativa (japonicahypothetical prote cultivar-group) 57 G343 gi12711287 1.60E−31 Nicotianatabacum GATA-1 zinc finger protein. 57 G343 gi1076609 4.40E−22 NicotianaNTL1 protein-curled- plumbaginifolia leaved to 57 G343 gi20372847 0.34Hordeum vulgare subsp. dof zinc finger protein. vulgare 57 G343 gi193220.41 Lycopersicon glycine-rich protein. esculentum 57 G343 gi214397540.55 Zea mays unnamed protein product. 57 G343 gi3219155 0.55Mesembryanthemum transcription factor Vp1. crystallinum 57 G343gi23504757 0.59 Pisum sativum nodule inception protein. 57 G343gi21439770 0.67 Triticum aestivum unnamed protein product. 59 G353BQ790831 5.00E−68 Brassica rapa subsp. E4675 Chinese cabbage pekinensisetiol 59 G353 BZ019752 1.00E−67 Brassica oleracea oed85c06.g1 B.oleracea002 Brassica olerac 59 G353 L46574 6.00E−40 Brassica rapaBNAF1975 Mustard flower buds Brassica rapa cD 59 G353 AB006601 7.00E−26Petunia x hybrida mRNA for ZPT2-14, complete cds. 59 G353 BM4371462.00E−25 Vitis vinifera VVA015A06_53787 An expressed sequence tag da 59G353 BI422808 1.00E−24 Lycopersicon EST533474 tomato callus, esculentumTAMU Lycop 59 G353 BU867080 1.00E−24 Populus tremula x S074B01 Populusimbib Populus tremuloides 59 G353 BM527789 3.00E−23 Glycine maxsal65h07.y1 Gm-c1061 Glycine max cDNA clone SOY 59 G353 BQ9802465.00E−23 Lactuca sativa QGE10I12.yg.ab1 QG_EFGHJ lettuce serriola La 59G353 BQ121106 2.00E−22 Solanum tuberosum EST606682 mixed potato tissuesSolanum tu 59 G353 gi2346976 6.50E−28 Petunia x hybrida ZPT2-13. 59 G353gi15623820 4.40E−25 Oryza sativa hypothetical protein. 59 G353gi21104613 1.40E−18 Oryza sativa (japonica contains ESTs AU07cultivar-group) 59 G353 gi485814 3.10E−13 Triticum aestivum WZF1. 59G353 gi7228329 4.00E−12 Medicago sativa putative TFIIIA (orkruppel)-like zinc fi 59 G353 gi1763063 1.70E−11 Glycine max SCOF-1. 59G353 gi2981169 2.60E−11 Nicotiana tabacum osmotic stress-induced zinc-finger prot 59 G353 gi4666360 1.10E−10 Datisca glomerata zinc-fingerprotein 1. 59 G353 gi2129892 2.30E−08 Pisum sativum probable fingerprotein Pszf1-garden pea. 59 G353 gi2058504 0.00018 Brassica rapazinc-finger protein-1. 61 G354 BZ083260 5.00E−49 Brassica oleracealle29f02.g1 B. oleracea002 Brassica olerac 61 G354 BQ790831 8.00E−45Brassica rapa subsp. E4675 Chinese cabbage pekinensis etiol 61 G354AB006600 6.00E−27 Petunia x hybrida mRNA for ZPT2-13, complete cds. 61G354 L46574 1.00E−26 Brassica rapa BNAF1975 Mustard flower buds Brassicarapa cD 61 G354 BM437146 3.00E−24 Vitis vinifera VVA015A06_53787 Anexpressed sequence tag da 61 G354 BQ121105 6.00E−24 Solanum tuberosumEST606681 mixed potato tissues Solanum tu 61 G354 BM527789 2.00E−23Glycine max sal65h07.y1 Gm-c1061 Glycine max cDNA clone SOY 61 G354AI898309 2.00E−23 Lycopersicon EST267752 tomato ovary, esculentum TAMULycope 61 G354 BU867080 5.00E−22 Populus tremula x S074B01 Populus imbibPopulus tremuloides 61 G354 BQ980246 1.00E−21 Lactuca sativaQGE10I12.yg.ab1 QG_EFGHJ lettuce serriola La 61 G354 gi2346976 5.60E−29Petunia x hybrida ZPT2-13. 61 G354 gi15623820 1.90E−22 Oryza sativahypothetical protein. 61 G354 gi21104613 4.00E−19 Oryza sativa (japonicacontains ESTs AU07 cultivar-group) 61 G354 gi2981169 1.80E−17 Nicotianatabacum osmotic stress-induced zinc- finger prot 61 G354 gi17630634.10E−16 Glycine max SCOF-1. 61 G354 gi4666360 8.90E−15 Datiscaglomerata zinc-finger protein 1. 61 G354 gi2058504 1.00E−14 Brassicarapa zinc-finger protein-1. 61 G354 gi7228329 4.90E−14 Medicago sativaputative TFIIIA (or kruppel)-like zinc fi 61 G354 gi485814 3.20E−13Triticum aestivum WZF1. 61 G354 gi2129892 1.20E−06 Pisum sativumprobable finger protein Pszf1-garden pea. 63 G361 BG135559 1.00E−24Lycopersicon EST468451 tomato crown esculentum gall Lycoper 63 G361AW686309 4.00E−23 Medicago truncatula NF036D10NR1F1000 Nodulated rootMedicag 63 G361 BU891880 8.00E−23 Populus tremula P056E03 Populuspetioles cDNA library Popul 63 G361 BU877646 2.00E−22 Populusbalsamifera V037D09 Populus flow subsp. trichocarpa 63 G361 BH7251349.00E−22 Brassica oleracea BOHWL71TF BO_2_3_KB Brassica oleracea gen 63G361 BI426538 2.00E−21 Glycine max sag04d12.y1 Gm-c1080 Glycine max cDNAclone GEN 63 G361 AP003214 2.00E−21 Oryza sativa chromosome 1 cloneOSJNBa0083M16, *** SEQUENCI 63 G361 AAAA01004859 3.00E−21 Oryza sativa(indica ( ) scaffold004859 cultivar-group) 63 G361 BU494379 1.00E−20Lotus japonicus Ljirnpest50-154-h2 Ljirnp Lambda HybriZap t 63 G361BQ488216 2.00E−17 Beta vulgaris 35-E8143-006-003-J02-T3 Sugar beetMPIZ-ADIS- 63 G361 gi15528588 4.00E−29 Oryza sativa hypotheticalprotein. 63 G361 gi18390109 2.80E−13 Sorghum bicolor putative zincfinger protein. 63 G361 gi18674684 1.50E−07 Zea ramosa unnamed proteinproduct. 63 G361 gi14275902 6.10E−07 Petunia x hybrida lateral shootinducing factor. 63 G361 gi21104613 0.00024 Oryza sativa (japonicacontains ESTs AU07 cultivar-group) 63 G361 gi2129892 0.00062 Pisumsativum probable finger protein Pszf1-garden pea. 63 G361 gi20585040.0018 Brassica rapa zinc-finger protein-1. 63 G361 gi4666360 0.018Datisca glomerata zinc-finger protein 1. 63 G361 gi7228329 0.047Medicago sativa putative TFIIIA (or kruppel)-like zinc fi 63 G361gi1763063 0.084 Glycine max SCOF-1. 65 G362 BF645161 6.00E−21 Medicagotruncatula NF031C06EC1F1049 Elicited cell culture 65 G362 BI2069036.00E−21 Lycopersicon EST524943 cTOS esculentum Lycopersicon esculen 65G362 BG047435 1.00E−18 Glycine max saa71c12.y1 Gm-c1060 Glycine max cDNAclone GEN 65 G362 BU877646 2.00E−15 Populus balsamifera V037D09 Populusflow subsp. trichocarpa 65 G362 BU891880 2.00E−15 Populus tremulaP056E03 Populus petioles cDNA library Popul 65 G362 AP003214 3.00E−13Oryza sativa chromosome 1 clone OSJNBa0083M16, *** SEQUENCI 65 G362AAAA01004859 3.00E−13 Oryza sativa (indica ( ) scaffold004859cultivar-group) 65 G362 BE358938 2.00E−11 Sorghum bicolorDG1_37_E12.b1_A002 Dark Grown 1 (DG1) Sorgh 65 G362 BQ488435 2.00E−11Beta vulgaris 05-E8886-006-003-J02-T3 Sugar beet MPIZ-ADIS- 65 G362BU494379 3.00E−11 Lotus japonicus Ljirnpest50-154-h2 Ljirnp LambdaHybriZap t 65 G362 gi15528588 2.70E−18 Oryza sativa hypotheticalprotein. 65 G362 gi2346984 9.00E−09 Petunia x hybrida ZPT2-9. 65 G362gi18390109 9.90E−08 Sorghum bicolor putative zinc finger protein. 65G362 gi21104613 0.00015 Oryza sativa (japonica contains ESTs AU07cultivar-group) 65 G362 gi18674684 0.0028 Zea ramosa unnamed proteinproduct. 65 G362 gi7228329 0.0029 Medicago sativa putative TFIIIA (orkruppel)-like zinc fi 65 G362 gi1763063 0.0039 Glycine max SCOF-1. 65G362 gi485814 0.0062 Triticum aestivum WZF1. 65 G362 gi4666360 0.0072Datisca glomerata zinc-finger protein 1. 65 G362 gi2058504 0.019Brassica rapa zinc-finger protein-1. 67 G371 CA799489 2.00E−38 Glycinemax sat34e06.y1 Gm-c1056 Glycine max cDNA clone SOY 67 G371 AF2656642.00E−32 Solanum tuberosum resistance gene cluster, complete sequenc 67G371 AJ497824 2.00E−31 Medicago truncatula AJ497824 MTFLOW Medicagotruncatula cDN 67 G371 AY129244 4.00E−31 Populus x canescens putativeRING protein (RING) mRNA, comp 67 G371 BM985575 1.00E−30 Thellungiellahalophila 1_F12_T3 Ath Thellungiella halophil 67 G371 BF051105 2.00E−30Lycopersicon EST436280 tomato esculentum developing/immatur 67 G371BU834871 2.00E−30 Populus tremula x T066G02 Populus apica Populustremuloides 67 G371 BM300635 5.00E−25 Mesembryanthemum MCA054H03_21640Ice crystallinum plant Lam 67 G371 BQ586594 1.00E−24 Beta vulgarisE012388-024-012-I21-SP6 MPIZ-ADIS-024-leaf Be 67 G371 BU880207 1.00E−24Populus balsamifera UM42TH03 Populus flo subsp. trichocarpa 67 G371gi22795037 8.80E−24 Populus x canescens putative RING protein. 67 G371gi15289911 2.20E−21 Oryza sativa hypothetical protein~similar toArabidopsis 67 G371 gi22535577 2.20E−21 Oryza sativa (japonicahypothetical prote cultivar-group) 67 G371 gi7688063 0.00026 Pisumsativum constitutively photomorphogenic 1 protein. 67 G371 gi181292860.0057 Pinus pinaster putative RING zinc finger protein. 67 G371gi22775495 0.014 Arabis gemmifera similar to A. thaliana AT4g08590. 67G371 gi15029364 0.015 Rosa hybrid cultivar photoregulatory zinc-fingerprotein 67 G371 gi7592844 0.025 Oryza sativa subsp. COP1. japonica 67G371 gi25044835 0.059 Ananas comosus RING zinc finger protein. 67 G371gi11127996 0.12 Ipomoea nil COP1. 69 G390 AB084381  1.0e−999 Zinniaelegans ZeHB-11 mRNA for homoeobox leucine-zipper pr 69 G390 AB032182 1.0e−999 Physcomitrella patens PpHB10 mRNA for homeobox protein PpHB 69G390 AY105765  1.0e−999 Zea mays PCO144112 mRNA sequence. 69 G390AAAA01006159  1.0e−999 Oryza sativa (indica ( ) scaffold006159cultivar-group) 69 G390 AP003197 1.00E−177 Oryza sativa chromosome 1clone B1015E06, *** SEQUENCING IN 69 G390 BQ857624 1.00E−106 Lactucasativa QGB8A10.yg.ab1 QG_ABCDI lettuce salinas Lact 69 G390 BI9255511.00E−101 Lycopersicon EST545440 tomato flower, esculentum buds 0-3 m 69G390 AW686191 1.00E−100 Medicago truncatula NF035A10NR1F1000 Nodulatedroot Medicag 69 G390 CA032516 1.00E−90 Hordeum vulgare subsp. HX13F16rHX Hordeum vulgare vulgare 69 G390 BQ116871 8.00E−90 Solanum tuberosumEST602447 mixed potato tissues Solanum tu 69 G390 gi24417149 1.00E−299Zinnia elegans homoeobox leucine-zipper protein. 69 G390 gi133843708.40E−280 Oryza sativa putative homeodomain- leucine zipper protein. 69G390 gi24431605 4.10E−274 Oryza sativa (japonica Putative homeodomacultivar-group) 69 G390 gi7209912 2.80E−244 Physcomitrella patenshomeobox protein PpHB10. 69 G390 gi3868829 4.50E−32 Ceratopterisrichardii CRHB1. 69 G390 gi19070143 5.00E−22 Picea abies homeodomainprotein HB2. 69 G390 gi1173622 1.10E−21 Phalaenopsis sp. homeoboxprotein. SM9108 69 G390 gi2147484 1.10E−21 Phalaenopsis sp. homeoticprotein, ovule- specific-Phala 69 G390 gi8920427 2.30E−20 Zea mays OCL5protein. 69 G390 gi18481701 7.70E−19 Sorghum bicolor OCL5 protein. 71G391 AB084381  1.0e−999 Zinnia elegans ZeHB-11 mRNA for homoeoboxleucine-zipper pr 71 G391 AB032182  1.0e−999 Physcomitrella patensPpHB10 mRNA for homeobox protein PpHB 71 G391 AY105765  1.0e−999 Zeamays PCO144112 mRNA sequence. 71 G391 AAAA01006159 1.00E−146 Oryzasativa (indica ( ) scaffold006159 cultivar-group) 71 G391 BQ8576241.00E−111 Lactuca sativa QGB8A10.yg.ab1 QG_ABCDI lettuce salinas Lact 71G391 AP003197 1.00E−106 Oryza sativa chromosome 1 clone B1015E06, ***SEQUENCING IN 71 G391 BI925551 1.00E−102 Lycopersicon EST545440 tomatoflower, esculentum buds 0-3 m 71 G391 AW686191 1.00E−102 Medicagotruncatula NF035A10NR1F1000 Nodulated root Medicag 71 G391 CA0325161.00E−92 Hordeum vulgare subsp. HX13F16r HX Hordeum vulgare vulgare 71G391 BQ116871 6.00E−91 Solanum tuberosum EST602447 mixed potato tissuesSolanum tu 71 G391 gi24417149 5.3e−310 Zinnia elegans homoeoboxleucine-zipper protein. 71 G391 gi13384370 3.20E−296 Oryza sativaputative homeodomain- leucine zipper protein. 71 G391 gi244316057.10E−283 Oryza sativa (japonica Putative homeodoma cultivar-group) 71G391 gi7209912 4.60E−255 Physcomitrella patens homeobox protein PpHB10.71 G391 gi3868829 6.30E−33 Ceratopteris richardii CRHB1. 71 G391gi18481701 9.10E−24 Sorghum bicolor OCL5 protein. 71 G391 gi120028533.50E−23 Picea abies homeobox 1. 71 G391 gi1173622 1.20E−22 Phalaenopsissp. homeobox protein. SM9108 71 G391 gi2147484 1.20E−22 Phalaenopsis sp.homeotic protein, ovule- specific-Phala 71 G391 gi8920427 9.30E−22 Zeamays OCL5 protein. 73 G409 BG044206 2.00E−66 Glycine max saa25c02.y1Gm-c1059 Glycine max cDNA clone GEN 73 G409 AF443621 3.00E−66Craterostigma homeodomain leucine plantagineum zipper prote 73 G409AW220361 6.00E−60 Lycopersicon EST302844 tomato root esculentumduring/after 73 G409 AF402606 5.00E−58 Phaseolus vulgaris homeodomainleucine zipper protein HDZ3 73 G409 AY105265 2.00E−56 Zea mays PCO062717mRNA sequence. 73 G409 BQ165293 2.00E−51 Medicago truncatula EST611162KVKC Medicago truncatula cDNA 73 G409 BH570275 1.00E−50 Brassicaoleracea BOHAF65TF BOHA Brassica oleracea genomic 73 G409 BF6203801.00E−48 Hordeum vulgare HVSMEc0019K16f Hordeum vulgare seedling sho 73G409 BF588126 2.00E−48 Sorghum propinquum FM1_38_A10.b1_A003Floral-Induced Merist 73 G409 AF145729 5.00E−45 Oryza sativa homeodomainleucine zipper protein (hox5) mRNA 73 G409 gi18034441 4.10E−65Craterostigma homeodomain leucine plantagineum zipper pro 73 G409gi15148920 1.10E−57 Phaseolus vulgaris homeodomain leucine zipperprotein HDZ 73 G409 gi5006855 7.20E−45 Oryza sativa homeodomain leucinezipper protein. 73 G409 gi1435021 9.00E−38 Daucus carota DNA-bindingprotein. 73 G409 gi6018089 1.50E−37 Glycine max homeodomain-leucinezipper protein 57. 73 G409 gi1161575 2.20E−36 Lycopersicon homeobox.esculentum 73 G409 gi11231065 1.40E−34 Zinnia elegans homeobox-leucinezipper protein. 73 G409 gi7415614 1.40E−34 Physcomitrella patenshomeobox protein PpHB1. 73 G409 gi8133126 4.10E−33 Brassica rapa subsp.hb-6-like protein. pekinensis 73 G409 gi22651698 1.80E−32 Nicotianatabacum homeodomain protein Hfi22. 75 G427 MDKNOX1 1.00E−143 Malusdomestica M. domestica mRNA for knotted1-like homeobox 75 G427 AB0047971.00E−136 Nicotiana tabacum NTH23 mRNA, complete cds. 75 G427 LEU764091.00E−132 Lycopersicon homeobox 1 protein esculentum (THox1) mRNA, pa 75G427 AB043957 1.00E−118 Ceratopteris richardii mRNA for CRKNOX3,complete cds. 75 G427 AW560103 1.00E−115 Medicago truncatula EST315151DSIR Medicago truncatula cDNA 75 G427 AB061818 1.00E−112 Oryza sativaHOS59 mRNA for KNOX family class 2 homeodomain 75 G427 BQ8739241.00E−100 Lactuca sativa QGI2O22.yg.ab1 QG_ABCDI lettuce salinas Lact 75G427 BNHDIBOX 9.00E−99 Brassica napus B. napus hd1 mRNA forhomeodomain-containing 75 G427 AY104273 8.00E−93 Zea mays PCO147946 mRNAsequence. 75 G427 BM063854 1.00E−91 Capsicum annuum KS01060C11 KS01Capsicum annuum cDNA, mRNA 75 G427 gi1946222 5.10E−131 Malus domesticaknotted1-like homeobox protein. 75 G427 gi3116212 3.40E−125 Nicotianatabacum homeobox gene. 75 G427 gi4098244 8.10E−124 Lycopersicon homeobox1 protein. esculentum 75 G427 gi1805618 3.60E−121 Oryza sativa OSH45transcript. 75 G427 gi11463943 2.50E−113 Ceratopteris richardii CRKNOX3.75 G427 gi1076449 1.40E−94 Brassica napus homeodomain-containingprotein-rape. 75 G427 gi14348597 1.00E−93 Physcomitrella patens class 2KNOTTED1-like protein MKN1- 75 G427 gi6016216 2.80E−43 Zea mays HOMEOBOXPROTEIN KNOTTED-1 LIKE 2. 75 G427 gi20977642 1.70E−34 Helianthus annuusknotted-1-like protein 1. 75 G427 gi3327269 6.50E−34 Ipomoea nil PKn1.77 G438 ZEL312053  1.0e−999 Zinnia elegans mRNA for HD-Zip protein (hb1gene). 77 G438 AB032182  1.0e−999 Physcomitrella patens PpHB10 mRNA forhomeobox protein PpHB 77 G438 AY105765  1.0e−999 Zea mays PCO144112 mRNAsequence. 77 G438 AAAA01006159 1.00E−165 Oryza sativa (indica ( )scaffold006159 cultivar-group) 77 G438 BU002601 1.00E−120 Lactuca sativaQGG31N03.yg.ab1 QG_EFGHJ lettuce serriola La 77 G438 BE035416 1.00E−106Mesembryanthemum MO05A06 MO crystallinum Mesembryanthemum c 77 G438BQ578798 1.00E−104 Triticum aestivum WHE0309_H06_O11ZS Wheat unstressedseedli 77 G438 BU927293 1.00E−103 Glycine max sas97g12.y1 Gm-c1036Glycine max cDNA clone SOY 77 G438 AW696625 1.00E−102 Medicagotruncatula NF109B06ST1F1048 Developing stem Medica 77 G438 BU0419057.00E−89 Prunus persica PP_LEa0010O09f Peach developing fruit mesoca 77G438 gi18076736  1.0e−999 Zinnia elegans HD-Zip protein. 77 G438gi13384370  1.0e−999 Oryza sativa putative homeodomain- leucine zipperprotein. 77 G438 gi24431605  3.3e−317 Oryza sativa (japonica Putativehomeodoma cultivar-group) 77 G438 gi7209912 4.90E−238 Physcomitrellapatens homeobox protein PpHB10. 77 G438 gi3868829 3.40E−35 Ceratopterisrichardii CRHB1. 77 G438 gi18481701 4.00E−21 Sorghum bicolor OCL5protein. 77 G438 gi1173622 8.50E−21 Phalaenopsis sp. homeobox protein.SM9108 77 G438 gi2147484 8.50E−21 Phalaenopsis sp. homeotic protein,ovule- specific-Phala 77 G438 gi12002853 1.40E−20 Picea abieshomeobox 1. 77 G438 gi8920427 3.20E−20 Zea mays OCL5 protein. 79 G450BQ155060 2.00E−84 Medicago truncatula NF075G11IR1F1088 IrradiatedMedicago tr 79 G450 PTR306829 5.00E−83 Populus tremula x mRNA foraux/IAA pro Populus tremuloides 79 G450 BE053029 1.00E−81 Gossypiumarboreum GA_Ea0031O18f Gossypium arboreum 7-10 d 79 G450 BI1791921.00E−79 Solanum tuberosum EST520137 cSTE Solanum tuberosum cDNA clo 79G450 BU006959 5.00E−78 Lactuca sativa QGH12O02.yg.ab1 QG_EFGHJ lettuceserriola La 79 G450 AF123508 8.00E−75 Nicotiana tabacum Nt-iaa28 deducedprotein mRNA, complete c 79 G450 BQ623078 2.00E−72 Citrus sinensisUSDA-FP_00169 Ridge pineapple sweet orange 79 G450 BI470140 7.00E−72Glycine max sah88c10.y1 Gm-c1050 Glycine max cDNA clone GEN 79 G450BU892057 7.00E−72 Populus tremula P058G09 Populus petioles cDNA libraryPopul 79 G450 AA427337 4.00E−71 Pisum sativum P482 Whero seedling lambdaZapII cDNA library 79 G450 gi20385508 4.20E−79 Populus tremula xauxin-regulated pro Populus tremuloides 79 G450 gi4887020 2.90E−73Nicotiana tabacum Nt-iaa28 deduced protein. 79 G450 gi114734 1.10E−69Glycine max AUXIN-INDUCED PROTEIN AUX28. 79 G450 gi22725714 2.00E−65Mirabilis jalapa auxin-responsive protein IAA1; MjAux/IAA 79 G450gi17976835 2.10E−61 Pinus pinaster putative auxin induced transcriptionfacto 79 G450 gi6136832 4.20E−57 Cucumis sativus CS-IAA2. 79 G450gi20257219 1.80E−56 Zinnia elegans auxin-regulated protein. 79 G450gi17154533 2.10E−54 Oryza sativa putative IAA1 protein. 79 G450gi22531416 5.30E−47 Gossypium hirsutum IAA16 protein. 79 G450 gi211047401.00E−43 Oryza sativa (japonica contains EST AU091 cultivar-group) 81G464 BH998146 2.00E−50 Brassica oleracea oef97f09.g1 B. oleracea002Brassica olerac 81 G464 BU043737 2.00E−44 Prunus persica PP_LEa0017A10fPeach 81developing fruit mesoca 81 G464 PTR306828 5.00E−44 Populustremula x mRNA for aux/IAA pro Populus tremuloides 81 G464 BI2075676.00E−44 Lycopersicon EST525607 cTOS esculentum Lycopersicon esculen 81G464 BQ592350 1.00E−35 Beta vulgaris E012681-024-020-J14-SP6MPIZ-ADIS-024-develop 81 G464 AV933892 4.00E−35 Hordeum vulgare subsp.AV933892 K. Sato vulgare unpublished 81 G464 BQ505545 5.00E−35 Solanumtuberosum EST612960 Generation of a set of potato c 81 G464 BE3640153.00E−34 Sorghum bicolor PI1_11_G02.b1_A002 Pathogen induced 1 (PI1) 81G464 BI118786 3.00E−34 Oryza sativa EST174 Differentially expressed cDNAlibraries 81 G464 AI725624 9.00E−32 Gossypium hirsutum BNLGHi12459Six-day Cotton fiber Gossypi 81 G464 gi20269057 1.60E−38 Populus tremulax aux/IAA protein. Populus tremuloides 81 G464 gi17976835 5.40E−32 Pinuspinaster putative auxin induced transcription facto 81 G464 gi51396972.00E−30 Cucumis sativus expressed in cucumber hypocotyls. 81 G464gi22725714 6.30E−30 Mirabilis jalapa auxin-responsive protein IAA1;MjAux/IAA 81 G464 gi17154533 1.30E−29 Oryza sativa putative IAA1protein. 81 G464 gi20257219 4.40E−29 Zinnia elegans auxin-regulatedprotein. 81 G464 gi2388689 4.40E−29 Glycine max GH1 protein. 81 G464gi16610193 1.10E−27 Nicotiana tabacum IAA9 protein. 81 G464 gi13520573.60E−27 Pisum sativum AUXIN-INDUCED PROTEIN IAA4. 81 G464 gi211047405.80E−27 Oryza sativa (japonica contains EST AU091 cultivar-group) 83G470 AB071293  1.0e−999 Oryza sativa OsARF2 mRNA for auxin responsefactor 2, parti 83 G470 OSA306306  1.0e−999 Oryza sativa (japonica Oryzasativa subsp. cultivar-group) 83 G470 AC126794  1.0e−999 Medicagotruncatula clone mth2-24j7, WORKING DRAFT SEQUENCE 83 G470 AY1062281.00E−131 Zea mays PCO137716 mRNA sequence. 83 G470 BQ578824 1.00E−118Triticum aestivum WHE0407_B10_D19ZS Wheat etiolated seedlin 83 G470BG045095 1.00E−108 Glycine max saa36f10.y1 Gm-c1059 Glycine max cDNAclone GEN 83 G470 CA030942 1.00E−102 Hordeum vulgare subsp. HX08J07r HXHordeum vulgare vulgare 83 G470 BI098203 4.00E−96 Sorghum bicolorIP1_29_D05.b1_A002 Immature pannicle 1 (IP1 83 G470 BG886848 5.00E−96Solanum tuberosum EST512699 cSTD Solanum tuberosum cDNA clo 83 G470AI774352 7.00E−95 Lycopersicon EST255368 tomato esculentum resistant,Cornell 83 G470 gi20805236 8.60E−223 Oryza sativa (japonica auxinresponse fac cultivar-group) 83 G470 gi19352039 6.10E−222 Oryza sativaauxin response factor 2. 83 G470 gi24785191 7.00E−70 Nicotiana tabacumhypothetical protein. 83 G470 gi23343944 5.70E−16 Mirabilis jalapaauxin-responsive factor protein. 83 G470 gi20269053 1.70E−08 Populustremula x aux/IAA protein. Populus tremuloides 83 G470 gi61368344.80E−07 Cucumis sativus CS-IAA3. 83 G470 gi287566 2.50E−06 Vignaradiata ORF. 83 G470 gi16610209 5.20E−06 Physcomitrella patens IAA/AUXprotein. 83 G470 gi114733 8.60E−06 Glycine max AUXIN-INDUCED PROTEINAUX22. 83 G470 gi18697008 4.00E−05 Zea mays unnamed protein product. 85G477 BH981212 8.00E−48 Brassica oleracea odf77g01.b1 B. oleracea002Brassica olerac 85 G477 BI925786 5.00E−39 Lycopersicon EST545675 tomatoflower, esculentum buds 0-3 m 85 G477 BM408208 7.00E−38 Solanumtuberosum EST582535 potato roots Solanum tuberosum 85 G477 BQ8748631.00E−30 Lactuca sativa QGI6H22.yg.ab1 QG_ABCDI lettuce salinas Lact 85G477 AMA011622 4.00E−30 Antirrhinum majus mRNA for squamosa promoterbinding 85 G477 BQ594361 4.00E−30 Beta vulgaris S015246-024-024-K10-SP6MPIZ-ADIS-024-develop 85 G477 CA516258 1.00E−28 Capsicum annuumKS09055D03 KS09 Capsicum annuum cDNA, mRNA 85 G477 BU828403 2.00E−28Populus tremula x K022P59P Populus apic Populus tremuloides 85 G477BG442540 2.00E−28 Gossypium arboreum GA_Ea0017G06f Gossypium arboreum7-10 d 85 G477 AW331087 7.00E−28 Zea mays 707047A12.x1 707-Mixed adulttissues from Walbot 85 G477 gi5931641 9.90E−32 Antirrhinum majussquamosa promoter binding protein-homol 85 G477 gi5931784 1.50E−28 Zeamays SBP-domain protein 4. 85 G477 gi8468036 4.40E−28 Oryza sativaSimilar to Arabidopsis thaliana chromosome 2 85 G477 gi9087308 1.20E−14Mitochondrion Beta orf102a. vulgaris var. altissima 85 G477 gi236305090.78 Triticum aestivum zinc finger protein. 85 G477 gi14597634 1Physcomitrella patens 15_ppprotl_080_c02. 87 G481 BU238020 9.00E−71Descurainia sophia Ds01_14a12_A Ds01_AAFC_ECORC_cold_stress 87 G481BG440251 2.00E−56 Gossypium arboreum GA_Ea0006K20f Gossypium arboreum7-10 d 87 G481 BF071234 1.00E−54 Glycine max st06h05.y1 Gm-c1065 Glycinemax cDNA clone GENO 87 G481 BQ799965 2.00E−54 Vitis vinifera EST 2134Green Grape berries Lambda Zap II L 87 G481 BQ488908 5.00E−53 Betavulgaris 95-E9134-006-006-M23-T3 Sugar beet MPIZ-ADIS- 87 G481 BU4994571.00E−52 Zea mays 946175D02.y1 946-tassel primordium prepared by S 87G481 AI728916 2.00E−52 Gossypium hirsutum BNLGHi12022 Six-day Cottonfiber Gossypi 87 G481 BG642751 3.00E−52 Lycopersicon EST510945 tomatoesculentum shoot/meristem Lyc 87 G481 BQ857127 3.00E−51 Lactuca sativaQGB6K24.yg.ab1 QG_ABCDI lettuce salinas Lact 87 G481 BE413647 6.00E−51Triticum aestivum SCU001.E10.R990714 ITEC SCU Wheat Endospe 87 G481gi115840 1.90E−51 Zea mays CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT A(CB 87 G481 gi20160792 2.60E−47 Oryza sativa (japonica putative CAAT-boxcultivar-group) 87 G481 gi15408794 7.10E−38 Oryza sativa putativeCCAAT-binding transcription factor 87 G481 gi22536010 3.20E−35 Phaseoluscoccineus LEC1-like protein. 87 G481 gi16902054 1.80E−32 Vernoniagalamensis CCAAT-box binding factor HAP3 B domai 87 G481 gi169020506.10E−32 Glycine max CCAAT-box binding factor HAP3 B domain. 87 G481gi16902056 1.60E−31 Argemone mexicana CCAAT-box binding factor HAP3 Bdomain. 87 G481 gi16902058 2.20E−27 Triticum aestivum CCAAT-box bindingfactor HAP3 B domain. 87 G481 gi388257 0.26 Lycopersicon glycine-richprotein. esculentum 87 G481 gi18266049 0.92 Brassica oleracea myrosinaseprecursor. 89 G482 BQ505706 7.00E−59 Solanum tuberosum EST613121Generation of a set of potato c 89 G482 AC122165 6.00E−57 Medicagotruncatula clone mth2-32m22, WORKING DRAFT SEQUENC 89 G482 BQ1046712.00E−55 Rosa hybrid cultivar fc0546.e Rose Petals (Fragrant Cloud) 89G482 BI469382 4.00E−55 Glycine max sai11b10.y1 Gm-c1053 Glycine max cDNAclone GEN 89 G482 AAAA01003638 1.00E−54 Oryza sativa (indica ( )scaffold003638 cultivar-group) 89 G482 AP005193 1.00E−54 Oryza sativa(japonica ( ) chromosome 7 clo cultivar-group) 89 G482 BU880488 1.00E−53Populus balsamifera UM49TG09 Populus flo subsp. trichocarpa 89 G482BJ248969 2.00E−53 Triticum aestivum BJ248969 Y. Ogihara unpublished cDNAlibr 89 G482 AC120529 4.00E−53 Oryza sativa chromosome 3 cloneOSJNBa0039N21, *** SEQUENCI 89 G482 BU896236 7.00E−53 Populus tremula xX037F04 Populus wood Populus tremuloides 89 G482 gi115840 1.40E−46 Zeamays CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT A (CB 89 G482 gi201607922.30E−41 Oryza sativa (japonica putative CAAT-box cultivar-group) 89G482 gi22536010 9.00E−38 Phaseolus coccineus LEC1-like protein. 89 G482gi15408794 1.50E−37 Oryza sativa putative CCAAT-binding transcriptionfactor 89 G482 gi16902054 7.50E−34 Vernonia galamensis CCAAT-box bindingfactor HAP3 B domai 89 G482 gi16902050 5.30E−33 Glycine max CCAAT-boxbinding factor HAP3 B domain. 89 G482 gi16902056 4.80E−32 Argemonemexicana CCAAT-box binding factor HAP3 B domain. 89 G482 gi169020581.10E−30 Triticum aestivum CCAAT-box binding factor HAP3 B domain. 89G482 gi100582 0.0018 Hordeum vulgare glycine-rich proteinprecursor-barley. 89 G482 gi7024451 0.0025 Citrus unshiu glycine-richRNA-binding protein. 91 G484 BQ412047 3.00E−68 Gossypium arboreumGA_Ed0053D06r Gossypium arboreum 7-10 d 91 G484 AF464906 5.00E−67Glycine max repressor protein (Dr1) mRNA, complete cds. 91 G484 AW7195752.00E−64 Lotus japonicus LjNEST6a11r Lotus japonicus nodule library, 91G484 BG648823 4.00E−64 Medicago truncatula EST510442 HOGA Medicagotruncatula cDNA 91 G484 BQ593791 4.00E−64 Beta vulgarisE012763-024-026-O09-SP6 MPIZ-ADIS-024-develop 91 G484 BM436739 9.00E−64Vitis vinifera VVA009B06_53061 An expressed sequence tag da 91 G484BF113032 1.00E−63 Lycopersicon EST440542 tomato breaker esculentum fruitLyco 91 G484 BG593107 7.00E−63 Solanum tuberosum EST491785 cSTS Solanumtuberosum cDNA clo 91 G484 BU014508 1.00E−61 Lactuca sativaQGJ7I14.yg.ab1 QG_EFGHJ lettuce serriola Lac 91 G484 AF464902 5.00E−59Oryza sativa repressor protein (Dr1) mRNA, complete cds. 91 G484gi18481628 6.70E−65 Glycine max repressor protein. 91 G484 gi184816204.80E−60 Oryza sativa repressor protein. 91 G484 gi18481622 2.00E−58Triticum aestivum repressor protein. 91 G484 gi20160792 2.90E−16 Oryzasativa (japonica putative CAAT-box cultivar-group) 91 G484 gi153217161.30E−15 Zea mays leafy cotyledon1. 91 G484 gi22536010 1.10E−14Phaseolus coccineus LEC1-like protein. 91 G484 gi16902054 1.50E−14Vernonia galamensis CCAAT-box binding factor HAP3 B domai 91 G484gi16902056 2.70E−13 Argemone mexicana CCAAT-box binding factor HAP3 Bdomain. 91 G484 gi18129292 1 Pinus pinaster histone H2B protein. 91 G484gi1083950 1 Canavalia lineata subtilisin inhibitor CLSI-I- Canavalia 93G489 BH679015 1.00E−111 Brassica oleracea BOHXO96TF BO_2_3_KB Brassicaoleracea gen 93 G489 AC136503 1.00E−75 Medicago truncatula clonemth2-15n1, WORKING DRAFT SEQUENCE 93 G489 BQ118033 8.00E−73 Solanumtuberosum EST603609 mixed potato tissues Solanum tu 93 G489 BU8735184.00E−68 Populus balsamifera Q056D09 Populus flow subsp. trichocarpa 93G489 BI934205 2.00E−67 Lycopersicon EST554094 tomato flower, esculentumanthesis L 93 G489 BQ797616 1.00E−66 Vitis vinifera EST 6554 RipeningGrape berries Lambda Zap I 93 G489 BM064398 4.00E−63 Capsicum annuumKS01066E11 KS01 Capsicum annuum cDNA, mRNA 93 G489 BU927107 4.00E−60Glycine max sas95f12.y1 Gm-c1036 Glycine max cDNA clone SOY 93 G489BQ993879 6.00E−59 Lactuca sativa QGF5L12.yg.ab1 QG_EFGHJ lettuceserriola Lac 93 G489 AP004113 1.00E−58 Oryza sativa chromosome 2 cloneOJ1116_A06, *** SEQUENCING 93 G489 gi5257260 6.20E−46 Oryza sativaSimilar to sequence of BAC F7G19 from Arabid 93 G489 gi20804442 6.60E−19Oryza sativa (japonica hypothetical prote cultivar-group) 93 G489gi18481626 3.90E−09 Zea mays repressor protein. 93 G489 gi1808688 0.051Sporobolus stapfianus hypothetical protein. 93 G489 gi8096192 0.21Lilium longiflorum gH2A.1. 93 G489 gi2130105 0.25 Triticum aestivumhistone H2A.4-wheat. 93 G489 gi297871 0.27 Picea abies histone H2A. 93G489 gi297887 0.31 Daucus carota glycine rich protein. 93 G489gi15214035 0.75 Cicer arietinum HISTONE H2A. 93 G489 gi2317760 0.75Pinus taeda H2A homolog. 95 G490 AX180963 1.00E−19 Physcomitrella patensSequence 14 from Patent WO0145493. 95 G490 AP004836 1.00E−19 Oryzasativa (japonica ( ) chromosome 2 clo cultivar-group) 95 G490 AU1976971.00E−19 Oryza sativa AU197697 Rice mature leaf Oryza sativa cDNA cl 95G490 BJ193952 1.00E−19 Physcomitrella patens BJ193952 normalized fulsubsp. patens 95 G490 AAAA01011976 1.00E−19 Oryza sativa (indica ( )scaffold011976 cultivar-group) 95 G490 BM065544 2.00E−19 Capsicum annuumKS07004F12 KS07 Capsicum annuum cDNA, mRNA 95 G490 AL749991 2.00E−19Pinus pinaster AL749991 AS Pinus pinaster cDNA clone AS03E0 95 G490BG440805 3.00E−19 Gossypium arboreum GA_Ea0010D12f Gossypium arboreum7-10 d 95 G490 BE460012 4.00E−19 Lycopersicon EST415304 tomatoesculentum developing/immatur 95 G490 BJ269516 4.00E−19 Triticumaestivum BJ269516 Y. Ogihara unpublished cDNA libr 95 G490 gi52572607.50E−18 Oryza sativa Similar to sequence of BAC F7G19 from Arabid 95G490 gi22138475 4.00E−13 Oryza sativa (japonica putative transcripcultivar-group) 95 G490 gi18481626 7.00E−06 Zea mays repressor protein.95 G490 gi16902058 0.99 Triticum aestivum CCAAT-box binding factor HAP3B domain. 95 G490 gi16902056 1 Argemone mexicana CCAAT-box bindingfactor HAP3 B domain. 95 G490 gi16902050 1 Glycine max CCAAT-box bindingfactor HAP3 B domain. 95 G490 gi16902054 1 Vernonia galamensis CCAAT-boxbinding factor HAP3 B domai 97 G504 BU895066 1.00E−82 Populus tremula xX018H04 Populus wood Populus tremuloides 97 G504 BI422750 2.00E−80Lycopersicon EST533416 tomato callus, esculentum TAMU Lycop 97 G504AW560823 5.00E−71 Medicago truncatula EST315871 DSIR Medicago truncatulacDNA 97 G504 CA815703 1.00E−68 Vitis vinifera CA12EI204IVF_E10 CabernetSauvignon Leaf-C 97 G504 BQ121923 2.00E−67 Solanum tuberosum EST607499mixed potato tissues Solanum tu 97 G504 BM092513 2.00E−66 Glycine maxsah14g06.y3 Gm-c1086 Glycine max cDNA clone GEN 97 G504 BI2460234.00E−66 Sorghum bicolor IP1_66_F11.b1_A002 Immature pannicle 1 (IP1 97G504 BU041353 1.00E−63 Prunus persica PP_LEa0009B03f Peach developingfruit mesoca 97 G504 BU672229 2.00E−63 Triticum aestivumWHE3302_A10_A20ZS Chinese Spring wheat dr 97 G504 AF402603 4.00E−62Phaseolus vulgaris NAC domain protein NAC2 mRNA, complete c 97 G504gi24417196 4.20E−72 Oryza sativa (japonica contains ESTs C993cultivar-group) 97 G504 gi15148914 2.70E−61 Phaseolus vulgaris NACdomain protein NAC2. 97 G504 gi15528779 3.50E−59 Oryza sativadevelopment regulation gene OsNAC4. 97 G504 gi6175246 2.50E−58Lycopersicon jasmonic acid 2. esculentum 97 G504 gi21105748 4.10E−58Petunia x hybrida nam-like protein 10. 97 G504 gi14485513 1.60E−56Solanum tuberosum putative NAC domain protein. 97 G504 gi42185352.10E−54 Triticum sp. GRAB1 protein. 97 G504 gi6732158 2.10E−54 Triticummonococcum unnamed protein product. 97 G504 gi22597158 2.90E−50 Glycinemax no apical meristem-like protein. 97 G504 gi7716952 2.20E−34 Medicagotruncatula NAC1. 99 G509 BG646875 2.00E−68 Medicago truncatula EST508494HOGA Medicago truncatula cDNA 99 G509 BQ850404 2.00E−65 Lactuca sativaQGB12I10.yg.ab1 QG_ABCDI lettuce salinas Lac 99 G509 BE363054 3.00E−59Sorghum bicolor DG1_9_D04.b1_A002 Dark Grown 1 (DG1) Sorghu 99 G509BE434322 1.00E−56 Lycopersicon EST405400 tomato breaker esculentumfruit, TIG 99 G509 BM112823 8.00E−50 Solanum tuberosum EST560359 potatoroots Solanum tuberosum 99 G509 AF402602 3.00E−49 Phaseolus vulgaris NACdomain protein NAC1 mRNA, complete c 99 G509 PHRNANAM 2.00E−48 Petunia xhybrida P. hybrida mRNA encoding NAM protein. 99 G509 BZ034968 4.00E−48Brassica oleracea oem78a04.b1 B. oleracea002 Brassica olerac 99 G509AV923588 3.00E−46 Hordeum vulgare subsp. AV923588 K. Sato vulgareunpublished 99 G509 BE586058 4.00E−46 Triticum aestivumEst#8pT7_C09_c9_066 KSU wheat Fusarium gr 99 G509 gi13129497 6.00E−57Oryza sativa putative NAM (no apical meristem) protein. 99 G509gi15148912 4.80E−50 Phaseolus vulgaris NAC domain protein NAC1. 99 G509gi24476048 3.30E−47 Oryza sativa (japonica Putative NAM (no acultivar-group) 99 G509 gi1279640 5.40E−47 Petunia x hybrida NAM. 99G509 gi4218537 8.50E−42 Triticum sp. GRAB2 protein. 99 G509 gi67321568.50E−42 Triticum monococcum unnamed protein product. 99 G509 gi225971581.40E−41 Glycine max no apical meristem-like protein. 99 G509 gi144855131.90E−37 Solanum tuberosum putative NAC domain protein. 99 G509gi6175246 8.40E−35 Lycopersicon jasmonic acid 2. esculentum 99 G509gi7716952 4.30E−32 Medicago truncatula NAC1. 101 G519 BG543276 9.00E−93Brassica rapa subsp. E0770 Chinese cabbage pekinensis etiol 101 G519BQ165234 2.00E−88 Medicago truncatula EST611103 KVKC Medicago truncatulacDNA 101 G519 AF509866 4.00E−85 Petunia x hybrida nam-like protein 3(NH3) mRNA, complete c 101 G519 STU401151 9.00E−85 Solanum tuberosummRNA for putative NAC domain protein (na 101 G519 BH476033 1.00E−80Brassica oleracea BOHNV27TF BOHN Brassica oleracea genomic 101 G519CA820578 2.00E−80 Glycine max sau91c12.y1 Gm-c1048 Glycine max cDNAclone SOY 101 G519 BM411425 1.00E−79 Lycopersicon EST585752 tomatobreaker esculentum fruit Lyco 101 G519 BQ970677 1.00E−78 Helianthusannuus QHB42M12.yg.ab1 QH_ABCDI sunflower RHA801 101 G519 AB0281852.00E−78 Oryza sativa mRNA for OsNAC6 protein, complete cds. 101 G519BG441329 6.00E−78 Gossypium arboreum GA_Ea0012N05f Gossypium arboreum7-10 d 101 G519 gi14485513 2.20E−86 Solanum tuberosum putative NACdomain protein. 101 G519 gi21105734 2.80E−86 Petunia x hybrida nam-likeprotein 3. 101 G519 gi13272281 1.40E−80 Oryza sativa NAC6. 101 G519gi20161457 1.40E−80 Oryza sativa (japonica OsNAC6 protein.cultivar-group) 101 G519 gi4218535 1.40E−62 Triticum sp. GRAB1 protein.101 G519 gi6732158 1.40E−62 Triticum monococcum unnamed protein product.101 G519 gi6175246 1.30E−54 Lycopersicon jasmonic acid 2. esculentum 101G519 gi15148914 4.30E−54 Phaseolus vulgaris NAC domain protein NAC2. 101G519 gi22597158 1.70E−43 Glycine max no apical meristem-like protein.101 G519 gi7716952 1.50E−35 Medicago truncatula NAC1. 103 G545 BH5526559.00E−96 Brassica oleracea BOGEH82TF BOGE Brassica oleracea genomic 103G545 BQ704580 7.00E−74 Brassica napus Bn01_02p11_A 103 G545 AF1190505.00E−59 Datisca glomerata zinc-finger protein 1 (zfp1) mRNA, comple 103G545 AP004523 9.00E−58 Lotus japonicus genomic DNA, chromosome 1, clone:LjT03J05, 103 G545 PETZFP4 2.00E−56 Petunia x hybrida Petuniazinc-finger protein gene. 103 G545 CA801331 4.00E−55 Glycine maxsau04c04.y2 Gm-c1062 Glycine max cDNA clone SOY 103 G545 MSY187881.00E−53 Medicago sativa mRNA for putative TFIIIA (or kruppel)-like 103G545 BG582865 2.00E−53 Medicago truncatula EST484611 GVN Medicagotruncatula cDNA 103 G545 BM437679 8.00E−51 Vitis viniferaVVA023E03_54853 An expressed sequence tag da 103 G545 AF053077 8.00E−49Nicotiana tabacum osmotic stress-induced zinc- finger protei 103 G545gi4666360 6.00E−57 Datisca glomerata zinc-finger protein 1. 103 G545gi7228329 2.70E−54 Medicago sativa putative TFIIIA (or kruppel)-likezinc fi 103 G545 gi1763063 9.00E−54 Glycine max SCOF-1. 103 G545gi439487 4.70E−44 Petunia x hybrida zinc-finger DNA binding protein. 103G545 gi2058504 1.50E−35 Brassica rapa zinc-finger protein-1. 103 G545gi2981169 4.30E−31 Nicotiana tabacum osmotic stress-induced zinc- fingerprot 103 G545 gi485814 6.50E−28 Triticum aestivum WZF1. 103 G545gi12698882 2.90E−25 Oryza sativa zinc finger transcription factor ZF1.103 G545 gi21104613 1.90E−14 Oryza sativa (japonica contains ESTs AU07cultivar-group) 103 G545 gi2129892 4.70E−06 Pisum sativum probablefinger protein Pszf1-garden pea. 105 G546 BG544345 3.00E−61 Brassicarapa subsp. E2200 Chinese cabbage pekinensis etiol 105 G546 BH4248546.00E−49 Brassica oleracea BOGML16TF BOGM Brassica oleracea genomic 105G546 AW223952 2.00E−45 Lycopersicon EST300763 tomato fruit redesculentum ripe, TA 105 G546 BG889076 4.00E−45 Solanum tuberosumEST514927 cSTD Solanum tuberosum cDNA clo 105 G546 AC127019 3.00E−44Medicago truncatula clone mth2-31b1, WORKING DRAFT SEQUENCE 105 G546BF597949 9.00E−42 Glycine max su89e06.y1 Gm-c1055 Glycine max cDNA cloneGENO 105 G546 BE033932 2.00E−40 Mesembryanthemum MG02C06 MG crystallinumMesembryanthemum c 105 G546 OSJN00157 3.00E−37 Oryza sativa chromosome 4clone OSJNBa0013K16, *** SEQUENC 105 G546 BI418846 3.00E−37 Lotusjaponicus LjNEST36e5r Lotus japonicus nodule library 105 G546AAAA01035793 3.00E−37 Oryza sativa (indica ( ) scaffold035793cultivar-group) 105 G546 gi2894379 3.10E−37 Hordeum vulgare ring fingerprotein. 105 G546 gi12039329 9.00E−34 Oryza sativa putative ring fingerprotein. 105 G546 gi19571069 1.80E−25 Oryza sativa (japonica containsEST C7268 cultivar-group) 105 G546 gi17016985 3.00E−23 Cucumis meloRING-H2 zinc finger protein. 105 G546 gi21645888 5.90E−18 Zea maysring-H2 zinc finger protein. 105 G546 gi23451086 2.10E−14 Medicagosativa RING-H2 protein. 105 G546 gi12003386 6.30E−14 Nicotiana tabacumAvr9/Cf-9 rapidly elicited protein 132. 105 G546 gi20152976 4.00E−12Hordeum vulgare subsp. similar to A. thaliana C3H vulgare 105 G546gi22597166 8.70E−12 Glycine max RING-H2 finger protein. 105 G546gi1086225 3.50E−09 Lotus japonicus RING-finger protein-Lotus japonicus.107 G561 SAY16953 1.00E−146 Sinapis alba mRNA for G-box binding factor2A. 107 G561 BNGBBF2A 1.00E−141 Brassica napus B. napus mRNA for G-Boxbinding factor 2A. 107 G561 RSGBOX 1.00E−141 Raphanus sativus R. sativusmRNA for G-box binding protein. 107 G561 PVU41817 8.00E−78 Phaseolusvulgaris regulator of MAT2 (ROM2) mRNA, complete 107 G561 AF0849717.00E−77 Catharanthus roseus G-box binding protein 1 (GBF1) mRNA, co 107G561 SOAJ3624 2.00E−75 Spinacia oleracea mRNA for basic leucine zipperprotein. 107 G561 SOYGBFB 1.00E−72 Glycine max G-box binding factor(GBF2A) mRNA, 3′ end. 107 G561 NTTAF2MR 2.00E−70 Nicotiana tabacum N.tabacum mRNA for TAF- 2. 107 G561 PCCPRF1 5.00E−66 Petroselinum crispumP. crispum CPRF1 mRNA for light-inducib 107 G561 ZMU10270 6.00E−49 Zeamays G-box binding factor 1 (GBF1) mRNA, complete cds. 107 G561gi2995462 1.00E−139 Sinapis alba G-box binding protein. 107 G561gi1076448 2.30E−135 Brassica napus G-box binding factor 2A- rape. 107G561 gi1033059 4.80E−135 Raphanus sativus G-Box binding protein. 107G561 gi1155054 2.30E−58 Phaseolus vulgaris regulator of MAT2. 107 G561gi5381311 3.50E−52 Catharanthus roseus G-box binding protein 1. 107 G561gi2815305 4.00E−51 Spinacia oleracea basic leucine zipper protein. 107G561 gi169959 1.20E−49 Glycine max G-box binding factor. 107 G561gi1076623 8.00E−46 Nicotiana tabacum G-box-binding protein TAF- 2-commonto 107 G561 gi498643 1.30E−45 Zea mays G-box binding factor 1. 107 G561gi100162 5.20E−42 Petroselinum crispum light-induced protein CPRF-1-parsl 109 G562 BNU27108 1.00E−160 Brassica napus transcription factor(BnGBF1) mRNA, partial 109 G562 AF084971 1.00E−102 Catharanthus roseusG-box binding protein 1 (GBF1) mRNA, co 109 G562 PVU41817 1.00E−96Phaseolus vulgaris regulator of MAT2 (ROM2) mRNA, complete 109 G562SOYGBFB 2.00E−94 Glycine max G-box binding factor (GBF2A) mRNA, 3′ end.109 G562 SOAJ3624 9.00E−94 Spinacia oleracea mRNA for basic leucinezipper protein. 109 G562 NTTAF2MR 4.00E−89 Nicotiana tabacum N. tabacummRNA for TAF- 2. 109 G562 PCCPRF1 1.00E−84 Petroselinum crispum P.crispum CPRF1 mRNA for light-inducib 109 G562 SAY16953 2.00E−81 Sinapisalba mRNA for G-box binding factor 2A. 109 G562 RSGBOX 6.00E−79 Raphanussativus R. sativus mRNA for G-box binding protein. 109 G562 BF2717906.00E−58 Gossypium arboreum GA_Eb0012L24f Gossypium arboreum 7-10 d 109G562 gi1399005 2.00E−159 Brassica napus transcription factor. 109 G562gi2995462 6.70E−81 Sinapis alba G-box binding protein. 109 G562gi1033059 1.80E−78 Raphanus sativus G-Box binding protein. 109 G562gi5381311 1.20E−60 Catharanthus roseus G-box binding protein 1. 109 G562gi2815305 1.20E−60 Spinacia oleracea basic leucine zipper protein. 109G562 gi1169081 2.20E−59 Petroselinum crispum COMMON PLANT REGULATORYFACTOR CPRF- 109 G562 gi169959 5.40E−56 Glycine max G-box bindingfactor. 109 G562 gi1155054 1.80E−55 Phaseolus vulgaris regulator ofMAT2. 109 G562 gi498643 2.10E−52 Zea mays G-box binding factor 1. 109G562 gi1076624 1.30E−47 Nicotiana tabacum G-box-binding protein TAF-3-common to 111 G567 PCCPRF2 1.00E−55 Petroselinum crispum P. crispumCPRF2 mRNA for DNA-binding p 111 G567 AY061648 8.00E−53 Nicotianatabacum bZIP transcription factor (BZI-1) mRNA, c 111 G567 BH5907392.00E−48 Brassica oleracea BOHCB55TR BOHC Brassica oleracea genomic 111G567 GMGHBF1 2.00E−47 Glycine max G. max mRNA for G/HBF-1. 111 G567RICBZIPPA 2.00E−44 Oryza sativa mRNA for bZIP protein, complete cds. 111G567 MZEBZIP 2.00E−43 Zea mays opaque2 heterodimerizing protein 2 mRNA,complete 111 G567 BU041142 3.00E−43 Prunus persica PP_LEa0008G18f Peachdeveloping fruit mesoca 111 G567 BG645542 4.00E−42 Medicago truncatulaEST507161 KV3 Medicago truncatula cDNA 111 G567 AJ487392 4.00E−41Solanum tuberosum AJ487392 Solanum tuberosum cv. Provita So 111 G567AW647973 9.00E−41 Lycopersicon EST326427 tomato esculentum germinatingseedli 111 G567 gi1806261 1.60E−49 Petroselinum crispum DNA-bindingprotein; bZIP type. 111 G567 gi1783305 1.80E−46 Oryza sativa bZIPprotein. 111 G567 gi16797791 8.20E−44 Nicotiana tabacum bZIPtranscription factor. 111 G567 gi168428 8.20E−44 Zea mays opaque2heterodimerizing protein 2. 111 G567 gi1905785 2.20E−43 Glycine maxG/HBF-1. 111 G567 gi1869928 9.70E−41 Hordeum vulgare blz-1 protein. 111G567 gi463212 4.40E−34 Coix lacryma-jobi opaque 2. 111 G567 gi13621781.00E−32 Sorghum bicolor opaque-2 protein-sorghum. 111 G567 gi214351012.90E−32 Pennisetum glaucum opaque-2-like protein. 111 G567 gi16540992.30E−24 Triticum aestivum transcriptional activator. 113 G568 BH9949721.00E−64 Brassica oleracea oeh20b03.b1 B. oleracea002 Brassica olerac113 G568 AF288616 2.00E−42 Populus balsamifera subsp. trichocarpa xPopulus deltoides 113 G568 BU834855 1.00E−25 Populus tremula x T066E09Populus apica Populus tremuloides 113 G568 BU819252 5.00E−23 Populustremula UA41BPE07 Populus tremula cambium cDNA libr 113 G568 AC1235717.00E−17 Medicago truncatula clone mth1-14n3, WORKING DRAFT SEQUENCE 113G568 AV914686 8.00E−14 Hordeum vulgare subsp. AV914686 K. Sato vulgareunpublished 113 G568 AF001454 8.00E−14 Helianthus annuus Dc3promoter-binding factor-2 (DPBF-2) mR 113 G568 BE657320 1.00E−13 Glycinemax GM700001A20B6 Gm- r1070 Glycine max cDNA clone G 113 G568 CA7654682.00E−13 Oryza sativa (indica AF53-Rpf_07_J23_T7_086 cultivar-group) 113G568 AL819191 2.00E−13 Triticum aestivum AL819191 n: 129 Triticumaestivum cDNA clo 113 G568 gi13435335 4.20E−47 Populus x generosa basicleucine zipper transcription fac 113 G568 gi22324425 6.30E−23 Oryzasativa (japonica bZIP transcription cultivar-group) 113 G568 gi22287733.30E−17 Helianthus annuus Dc3 promoter-binding factor-2. 113 G568gi21693583 8.70E−15 Triticum aestivum ABA response element bindingfactor. 113 G568 gi5821255 4.90E−13 Oryza sativa TRAB1. 113 G568gi13775111 4.20E−12 Phaseolus vulgaris bZIP transcription factor 6. 113G568 gi7406677 3.30E−11 Vitis vinifera putative ripening-related bZIPprotein. 113 G568 gi14571808 2.90E−10 Nicotiana tabacum phi-2. 113 G568gi6018699 3.10E−10 Lycopersicon THY5 protein. esculentum 113 G568gi1352613 3.20E−10 Zea mays OCS-ELEMENT BINDING FACTOR 1 (OCSBF-1). 115G584 PVU18348 1.00E−166 Phaseolus vulgaris phaseolin G-box bindingprotein PG1 (PG1 115 G584 BH696428 5.00E−94 Brassica oleracea BOMCR67TFBO_2_3_KB Brassica oleracea gen 115 G584 AF011557 7.00E−80 Lycopersiconjasmonic acid 3 (LEJA3) esculentum mRNA, parti 115 G584 BI4346519.00E−75 Solanum tuberosum EST537412 P. infestans- challenged leaf So115 G584 AF061107 2.00E−70 Zea mays transcription factor MYC7E mRNA,partial cds. 115 G584 BG453241 3.00E−70 Medicago truncatulaNF090G06LF1F1049 Developing leaf Medica 115 G584 AAAA01004195 2.00E−68Oryza sativa (indica ( ) scaffold004195 cultivar-group) 115 G584AC060755 6.00E−68 Oryza sativa chromosome 10 clone OSJNBa0003O19, ***SEQUENC 115 G584 BG446831 7.00E−67 Gossypium arboreum GA_Eb0039H18fGossypium arboreum 7-10 d 115 G584 BI968400 2.00E−62 Glycine maxGM830005A12E12 Gm- r1083 Glycine max cDNA clone 115 G584 gi11426193.90E−155 Phaseolus vulgaris phaseolin G-box binding protein PG1. 115G584 gi12643064 1.00E−131 Oryza sativa putative MYC transcriptionfactor. 115 G584 gi4321762 4.30E−130 Zea mays transcription factorMYC7E. 115 G584 gi6175252 2.30E−62 Lycopersicon jasmonic acid 3.esculentum 115 G584 gi19571087 2.70E−47 Oryza sativa (japonica containsEST AU031 cultivar-group) 115 G584 gi10998404 1.40E−37 Petunia x hybridaanthocyanin 1. 115 G584 gi4519201 9.30E−30 Perilla frutescens MYC-GP.115 G584 gi166428 8.00E−28 Antirrhinum majus DEL. 115 G584 gi133461823.00E−27 Gossypium hirsutum GHDEL65. 115 G584 gi3650292 5.10E−18 Gerberahybrida GMYC1 protein. 117 G585 AF336280 1.00E−165 Gossypium hirsutumGHDEL65 (ghdel65) mRNA, complete cds. 117 G585 AMADEL 1.00E−147Antirrhinum majus DEL (delila) mRNA, complete cds. 117 G585 AB0240501.00E−142 Perilla frutescens mRNA for MYC-RP, complete cds. 117 G585AF020545 1.00E−135 Petunia x hybrida bHLH transcription factor JAF13(jaf13) m 117 G585 GHY7709 1.00E−107 Gerbera hybrida mRNA for bHLHtranscription factor. 117 G585 AX540498 1.00E−104 Lotus uliginosusSequence 2 from Patent WO0210412. 117 G585 ZMA251719 9.00E−81 Zea maysmRNA for transcription factor (hopi gene). 117 G585 AF503363 3.00E−67Lotus japonicus myc-like regulatory protein (TAN1) mRNA, pa 117 G585BI308638 7.00E−67 Medicago truncatula EST530048 GPOD Medicago truncatulacDNA 117 G585 BU875274 1.00E−57 Populus balsamifera V004CE04 Populus flosubsp. trichocarpa 117 G585 gi13346182 6.30E−156 Gossypium hirsutumGHDEL65. 117 G585 gi166428 5.70E−139 Antirrhinum majus DEL. 117 G585gi4519199 2.60E−127 Perilla frutescens MYC-RP. 117 G585 gi31270455.40E−127 Petunia x hybrida bHLH transcription factor JAF13. 117 G585gi3650292 1.30E−93 Gerbera hybrida GMYC1 protein. 117 G585 gi80524572.00E−87 Zea mays transcription factor. 117 G585 gi1086540 2.20E−86Oryza sativa Ra. 117 G585 gi20467247 2.40E−83 Lotus uliginosus myc-likeregulatory protein. 117 G585 gi20467249 5.90E−66 Lotus japonicusmyc-like regulatory protein. 117 G585 gi21429235 1.70E−50 Onobrychisviciifolia basic helix-loop-helix regulatory p 119 G590 AW7821481.00E−49 Glycine max sm02b05.y1 Gm-c1027 Glycine max cDNA clone GENO 119G590 AW649972 5.00E−45 Lycopersicon EST328426 tomato esculentumgerminating seedli 119 G590 BZ045178 2.00E−37 Brassica oleracea1kf53d05.g1 B. oleracea002 Brassica olerac 119 G590 BM408345 3.00E−31Solanum tuberosum EST582672 potato roots Solanum tuberosum 119 G590BM065639 4.00E−31 Capsicum annuum KS07005G09 KS07 Capsicum annuum cDNA,mRNA 119 G590 BI308330 1.00E−30 Medicago truncatula EST529740 GPODMedicago truncatula cDNA 119 G590 BQ134415 5.00E−28 Zea mays1091016H12.y2 1091- Immature ear with common ESTs 119 G590 BU8660691.00E−25 Populus tremula x S062C11 Populus imbib Populus tremuloides 119G590 AU290290 1.00E−24 Zinnia elegans AU290290 zinnia cultured mesophyllcell equa 119 G590 BU574318 1.00E−24 Prunus dulcis PA_Ea0007A10f Almonddeveloping seed Prunus 119 G590 gi15451582 7.80E−32 Oryza sativaPutative SPATULA. 119 G590 gi23495742 8.20E−28 Oryza sativa (japonicaputative phytochro cultivar-group) 119 G590 gi5923912 5.40E−10 Tulipagesneriana bHLH transcription factor GBOF-1. 119 G590 gi527657 1.40E−09Pennisetum glaucum myc-like regulatory R gene product. 119 G590gi6166283 2.30E−09 Pinus taeda helix-loop-helix protein 1A. 119 G590gi527665 4.80E−09 Sorghum bicolor myc-like regulatory R gene product.119 G590 gi527661 1.00E−08 Phyllostachys acuta myc-like regulatory Rgene product. 119 G590 gi1086534 1.70E−08 Oryza officinalistranscriptional activator Ra homolog. 119 G590 gi1086526 2.80E−08 Oryzaaustraliensis transcriptional activator Ra homolog. 119 G590 gi10865384.60E−08 Oryza rufipogon transcriptional activator Rb homolog. 121 G594BE807866 4.00E−38 Glycine max ss31c06.y1 Gm-c1061 Glycine max cDNA cloneGENO 121 G594 BQ875608 5.00E−38 Lactuca sativa QGI8J14.yg.ab1 QG_ABCDIlettuce salinas Lact 121 G594 BU791131 1.00E−36 Populus balsamiferasubsp. trichocarpa x Populus deltoides 121 G594 CA015610 9.00E−35Hordeum vulgare subsp. HT14N12r HT Hordeum vulgare vulgare 121 G594BF200249 2.00E−34 Triticum monococcum WHE2254_F11_L22ZE Triticummonococcum s 121 G594 BM497415 6.00E−34 Avicennia marina 901269Avicennia marina leaf cDNA Library 121 G594 AW906522 4.00E−33 Solanumtuberosum EST342644 potato stolon, Cornell Universi 121 G594 AI7314175.00E−33 Gossypium hirsutum BNLGHi9478 Six-day Cotton fiber Gossypiu 121G594 BE455695 5.00E−33 Hordeum vulgare HVSMEg0019A10f Hordeum vulgarepre- anthesis 121 G594 BE360329 5.00E−33 Sorghum bicolorDG1_62_C04.g1_A002 Dark Grown 1 (DG1) Sorgh 121 G594 gi20804997 2.20E−34Oryza sativa (japonica DNA-binding protei cultivar-group) 121 G594gi11862964 6.00E−34 Oryza sativa hypothetical protein. 121 G594gi5923912 3.40E−31 Tulipa gesneriana bHLH transcription factor GBOF-1.121 G594 gi6166283 4.30E−10 Pinus taeda helix-loop-helix protein 1A. 121G594 gi13346182 3.80E−06 Gossypium hirsutum GHDEL65. 121 G594 gi5276654.80E−06 Sorghum bicolor myc-like regulatory R gene product. 121 G594gi527661 6.20E−06 Phyllostachys acuta myc-like regulatory R geneproduct. 121 G594 gi4206118 6.60E−06 Mesembryanthemum transporterhomolog. crystallinum 121 G594 gi527657 1.30E−05 Pennisetum glaucummyc-like regulatory R gene product. 121 G594 gi1086526 0.0001 Oryzaaustraliensis transcriptional activator Ra homolog. 123 G597 BE6008165.00E−62 Sorghum bicolor PI1_90_E07.b1_A002 Pathogen induced 1 (PI1) 123G597 AY106980 3.00E−60 Zea mays PCO106555 mRNA sequence. 123 G597BQ765321 3.00E−58 Hordeum vulgare EBro03_SQ006_H21_R root, 3 week,waterlogge 123 G597 CA501339 2.00E−57 Triticum aestivumWHE4032_D07_H14ZT Wheat meiotic anther cD 123 G597 BQ841090 1.00E−56Aegilops speltoides WHE4206_H10_O20ZS Aegilops speltoides p 123 G597BG465540 8.00E−56 Sorghum propinquum RHIZ2_45_G09.b1_A003 Rhizome2(RHIZ2) So 123 G597 AW928863 7.00E−53 Lycopersicon EST337651 tomatoflower esculentum buds 8 mm t 123 G597 BQ856774 4.00E−51 Lactuca sativaQGB5L17.yg.ab1 QG_ABCDI lettuce salinas Lact 123 G597 BU926769 5.00E−51Glycine max sas91d09.y1 Gm-c1036 Glycine max cDNA clone SOY 123 G597BJ473026 1.00E−50 Hordeum vulgare subsp. BJ473026 K. Sato vulgareunpublished 123 G597 gi12643044 1.60E−65 Oryza sativa putative AT-HookDNA- binding protein. 123 G597 gi2213536 3.20E−49 Pisum sativumDNA-binding protein PD1. 123 G597 gi4165183 2.90E−41 Antirrhinum majusSAP1 protein. 123 G597 gi24418033 4.20E−15 Oryza sativa (japonicaHypothetical prote cultivar-group) 123 G597 gi13992574 0.00058 Triticumtimopheevii glutenin HMW subunit 1Ax. 123 G597 gi100787 0.0011 Triticumaestivum glutenin high molecular weight chain 1A 123 G597 gi71887200.0032 Aegilops ventricosa x-type high molecular weight glutenin 123G597 gi456124 0.066 Nicotiana tabacum DNA-binding protein. 123 G597gi21218057 0.076 Chlamydomonas putative Pi-transporter reinhardtiihomolog 123 G597 gi21779920 0.14 Aegilops tauschii HMW-glutenin. 125G598 BH488116 9.00E−41 Brassica oleracea BOHPM37TF BOHP Brassicaoleracea genomic 125 G598 BG455043 9.00E−38 Medicago truncatulaNF112G09LF1F1069 Developing leaf Medica 125 G598 BQ856793 3.00E−35Lactuca sativa QGB5M13.yg.ab1 QG_ABCDI lettuce salinas Lact 125 G598AW932217 3.00E−33 Lycopersicon EST358060 tomato fruit esculentum maturegreen 125 G598 BQ511117 5.00E−31 Solanum tuberosum EST618532 Generationof a set of potato c 125 G598 AP003981 3.00E−30 Oryza sativa chromosome7 clone OJ1019_E02, *** SEQUENCING 125 G598 AAAA01001857 3.00E−30 Oryzasativa (indica ( ) scaffold001857 cultivar-group) 125 G598 AC1359587.00E−30 Oryza sativa (japonica ( ) chromosome 3 clo cultivar-group) 125G598 BG319716 9.00E−23 Zea mays Zm03_06a07_AZm03_AAFC_ECORC_cold_stressed_maize_s 125 G598 BU025013 2.00E−20Helianthus annuus QHF7D11.yg.ab1 QH_EFGHJ sunflower RHA280 125 G598gi1881585 0.059 Solanum tuberosum remorin. 125 G598 gi15289949 0.11Oryza sativa (japonica hypothetical prote cultivar-group) 125 G598gi4883530 0.32 Lycopersicon remorin 2. esculentum 125 G598 gi131613670.96 Oryza sativa hypothetical protein. 125 G598 gi13775109 0.97Phaseolus vulgaris bZIP transcription factor 3. 125 G598 gi8096269 0.98Nicotiana tabacum KED. 125 G598 gi2598161 0.98 Pinus strobusNADPH:protochlorophyllide oxidoreductase po 125 G598 gi1183880 0.99Brassica napus oleosin-like protein. 125 G598 gi22002966 1 Hordeumvulgare subsp. putative CENP-E like kinet vulgare 125 G598 gi4185307 1Zea mays unknown. 127 G634 OSGT2 2.00E−47 Oryza sativa O. sativa gt-2gene. 127 G634 BU049946 1.00E−46 Zea mays 1111017E09.y1 1111- UnigeneIII from Maize Genome 127 G634 AF372499 6.00E−38 Glycine max GT-2 factormRNA, partial cds. 127 G634 AB052729 4.00E−37 Pisum sativum mRNA forDNA-binding protein DF1, complete cd 127 G634 BU889446 4.00E−36 Populustremula P021A05 Populus petioles cDNA library Popul 127 G634 BH4369582.00E−35 Brassica oleracea BOHBE67TF BOHB Brassica oleracea genomic 127G634 AI777252 3.00E−35 Lycopersicon EST258217 tomato esculentumresistant, Cornell 127 G634 AW686754 1.00E−33 Medicago truncatulaNF042C08NR1F1000 Nodulated root Medicag 127 G634 AV410715 4.00E−33 Lotusjaponicus AV410715 Lotus japonicus young plants (two- 127 G634 AI7309338.00E−30 Gossypium hirsutum BNLGHi8208 Six-day Cotton fiber Gossypiu 127G634 gi13786451 3.20E−78 Oryza sativa putative transcription factor. 127G634 gi13646986 3.50E−66 Pisum sativum DNA-binding protein DF1. 127 G634gi18182311 2.70E−38 Glycine max GT-2 factor. 127 G634 gi201615678.90E−11 Oryza sativa (japonica hypothetical prote cultivar-group) 127G634 gi170271 4.70E−08 Nicotiana tabacum DNA-binding protein. 127 G634gi18349 0.0027 Daucus carota glycine rich protein (AA 1- 96). 127 G634gi21388658 0.027 Physcomitrella patens glycine-rich RNA binding protein.127 G634 gi21322752 0.052 Triticum aestivum cold shock protein-1. 127G634 gi3126963 0.057 Elaeagnus umbellata acidic chitinase. 127 G634gi1166450 0.087 Lycopersicon Tfm5. esculentum 129 G635 BH5283451.00E−117 Brassica oleracea BOGNZ34TR BOGN Brassica oleracea genomic 129G635 BQ916526 4.00E−71 Helianthus annuus QHB18C05.yg.ab1 QH_ABCDIsunflower RHA801 129 G635 AY110231 1.00E−68 Zea mays CL852_1 mRNAsequence. 129 G635 BI139375 3.00E−42 Populus balsamifera F130P49YPopulus flo subsp. trichocarpa 129 G635 BQ850859 3.00E−42 Lactuca sativaQGB13M04.yg.ab1 QG_ABCDI lettuce salinas Lac 129 G635 AC137603 6.00E−40Medicago truncatula clone mth2-14b10, WORKING DRAFT SEQUENC 129 G635BF269947 6.00E−37 Gossypium arboreum GA_Eb0006B11f Gossypium arboreum7-10 d 129 G635 AW760602 5.00E−34 Glycine max s152e02.y1 Gm-c1027Glycine max cDNA clone GENO 129 G635 BJ464004 1.00E−30 Hordeum vulgaresubsp. BJ464004 K. Sato vulgare unpublished 129 G635 AAAA010000071.00E−30 Oryza sativa (indica ( ) scaffold000007 cultivar-group) 129G635 gi21741458 3.30E−08 Oryza sativa OJ000223_09.14. 129 G635 gi1702711.20E−07 Nicotiana tabacum DNA-binding protein. 129 G635 gi181823093.00E−06 Glycine max GT-2 factor. 129 G635 gi13646986 3.10E−05 Pisumsativum DNA-binding protein DF1. 129 G635 gi22128704 0.02 Oryza sativa(japonica hypothetical prote cultivar-group) 129 G635 gi7208779 0.04Cicer arietinum hypothetical protein. 129 G635 gi1279563 0.056 Medicagosativa nuM1. 129 G635 gi15144506 0.066 Lycopersicon unknown. esculentum129 G635 gi349585 0.36 Volvox carteri histone H1-I. 129 G635 gi29112920.49 Capsicum annuum prosystemin. 131 G636 AB052729 1.00E−134 Pisumsativum mRNA for DNA-binding protein DF1, complete cd 131 G636 OSGT21.00E−109 Oryza sativa O. sativa gt-2 gene. 131 G636 AF372498 1.00E−103Glycine max GT-2 factor mRNA, partial cds. 131 G636 AAAA010171451.00E−101 Oryza sativa (indica ( ) scaffold017145 cultivar-group) 131G636 BH521870 4.00E−89 Brassica oleracea BOGMP76TF BOGM Brassicaoleracea genomic 131 G636 AP004868 2.00E−79 Oryza sativa (japonica ( )chromosome 2 clo cultivar-group) 131 G636 BU894555 2.00E−69 Populustremula x X011B09 Populus wood Populus tremuloides 131 G636 BG4468492.00E−57 Gossypium arboreum GA_Eb0039I22f Gossypium arboreum 7-10 d 131G636 AW032956 3.00E−52 Lycopersicon EST276515 tomato callus, esculentumTAMU Lycop 131 G636 AC135565 4.00E−49 Medicago truncatula clonemth2-19b12, WORKING DRAFT SEQUENC 131 G636 gi13646986 4.50E−111 Pisumsativum DNA-binding protein DF1. 131 G636 gi18182309 4.00E−99 Glycinemax GT-2 factor. 131 G636 gi13786451 5.30E−98 Oryza sativa putativetranscription factor. 131 G636 gi170271 4.30E−13 Nicotiana tabacumDNA-binding protein. 131 G636 gi20161567 4.00E−09 Oryza sativa (japonicahypothetical prote cultivar-group) 131 G636 gi10636140 0.00014 Aegilopsspeltoides gamma-gliadin. 131 G636 gi442524 0.00015 Hordeum vulgareC-hordein. 131 G636 gi15148391 0.00021 Triticum aestivum gamma-gliadin.131 G636 gi225589 0.00021 Hordeum vulgare var. hordein C. distichum 131G636 gi4584086 0.00061 Spermatozopsis similis p210 protein. 133 G638BZ034676 3.00E−87 Brassica oleracea oef83a05.g1 B. oleracea002 Brassicaolerac 133 G638 BQ866994 6.00E−55 Lactuca sativa QGC9I02.yg.ab1 QG_ABCDIlettuce salinas Lact 133 G638 BM110736 1.00E−54 Solanum tuberosumEST558272 potato roots Solanum tuberosum 133 G638 BF646615 9.00E−48Medicago truncatula NF066C08EC1F1065 Elicited cell culture 133 G638OSGT2 3.00E−36 Oryza sativa O. sativa gt-2 gene. 133 G638 AP0048684.00E−33 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 133G638 AB052729 2.00E−32 Pisum sativum mRNA for DNA-binding protein DF1,complete cd 133 G638 AI777252 4.00E−29 Lycopersicon EST258217 tomatoesculentum resistant, Cornell 133 G638 BM500043 2.00E−28 Zea mays952036C09.y1 952-BMS tissue from Walbot Lab (red 133 G638 AF3724995.00E−28 Glycine max GT-2 factor mRNA, partial cds. 133 G638 gi202492.00E−49 Oryza sativa gt-2. 133 G638 gi13646986 4.30E−45 Pisum sativumDNA-binding protein DF1. 133 G638 gi18182311 1.10E−30 Glycine max GT-2factor. 133 G638 gi20161567 2.60E−07 Oryza sativa (japonica hypotheticalprote cultivar-group) 133 G638 gi170271 3.40E−06 Nicotiana tabacumDNA-binding protein. 133 G638 gi21068672 3.60E−05 Cicer arietinumputative glicine-rich protein. 133 G638 gi20257673 4.60E−05 Zea maysglycine-rich RNA binding protein. 133 G638 gi21388660 0.00014Physcomitrella patens glycine-rich RNA-binding protein. 133 G638gi9755844 0.00033 Brassica napus putative glycine-rich protein. 133 G638gi1166450 0.00037 Lycopersicon Tfm5. esculentum 135 G652 BH9269805.00E−90 Brassica oleracea odi21g11.g1 B. oleracea002 Brassica olerac135 G652 NSGRP2MR 1.00E−71 Nicotiana sylvestris N. sylvestris mRNA forglycine rich pro 135 G652 AI812203 7.00E−65 Zea mays 605086G09.y1 605-Endosperm cDNA library from Sch 135 G652 BM408211 4.00E−64 Solanumtuberosum EST582538 potato roots Solanum tuberosum 135 G652 AP0038796.00E−64 Oryza sativa chromosome 8 clone OJ1123_A02, *** SEQUENCING 135G652 AP004591 6.00E−64 Oryza sativa (japonica ( ) chromosome 8 clocultivar-group) 135 G652 AAAA01000576 7.00E−63 Oryza sativa (indica ( )scaffold000576 cultivar-group) 135 G652 AB066265 1.00E−62 Triticumaestivum WCSP1 mRNA for cold shock protein-1, comp 135 G652 BQ8405772.00E−62 Aegilops speltoides WHE4201_B07_C13ZS Aegilops speltoides p 135G652 BE035242 1.00E−53 Mesembryanthemum MO03A01 MO crystallinumMesembryanthemum c 135 G652 gi121631 9.30E−68 Nicotiana sylvestrisGLYCINE-RICH CELL WALL STRUCTURAL PR 135 G652 gi21322752 1.70E−61Triticum aestivum cold shock protein-1. 135 G652 gi121628 5.00E−26Phaseolus vulgaris GLYCINE-RICH CELL WALL STRUCTURAL PROT 135 G652gi395147 7.10E−25 Nicotiana tabacum glycine-rich protein. 135 G652gi17821 1.40E−23 Brassica napus glycine-rich_protein_(aa1- 291). 135G652 gi121627 1.80E−23 Petunia x hybrida GLYCINE-RICH CELL WALLSTRUCTURAL PROTE 135 G652 gi225181 1.80E−23 Petunia sp. Gly richstructural protein. 135 G652 gi15528745 2.00E−22 Oryza sativa containsESTs AU093876(E1018), AU093877 (E1018 135 G652 gi21327989 2.00E−22 Oryzasativa (japonica contains ESTs AU09 cultivar-group) 135 G652 gi213886604.40E−22 Physcomitrella patens glycine-rich RNA-binding protein. 137G663 AF146702 6.00E−54 Petunia x hybrida An2 protein (an2) mRNA, an2-V26allele, c 137 G663 AF146703 3.00E−53 Petunia integrifolia An2 protein(an2) mRNA, an2-S9 allele, 137 G663 BQ990780 4.00E−51 Lactuca sativaQGF21B10.yg.ab1 QG_EFGHJ lettuce serriola La 137 G663 BE462282 3.00E−50Lycopersicon EST324546 tomato flower esculentum buds 0-3 mm 137 G663AB073013 6.00E−50 Vitis labrusca x Vitis VlmybA2 gene for myb- viniferarelate 137 G663 AF146709 2.00E−49 Petunia axillaris An2 truncatedprotein (an2) mRNA, an2-S7 137 G663 BH480961 3.00E−47 Brassica oleraceaBOGZT54TF BOGZ Brassica oleracea genomic 137 G663 BF635572 6.00E−42Medicago truncatula NF104H01DT1F1014 Drought Medicago trunc 137 G663BQ105368 2.00E−41 Rosa hybrid cultivar fc0707.e Rose Petals (FragrantCloud) 137 G663 AF336278 2.00E−41 Gossypium hirsutum BNLGHi233(bnlghi6233) mRNA, complete cd 137 G663 gi7673084 1.10E−53 Petunia xhybrida An2 protein. 137 G663 gi7673086 3.90E−53 Petunia integrifoliaAn2 protein. 137 G663 gi22266667 2.30E−50 Vitis labrusca x Vitismyb-related transcription vinifera 137 G663 gi7673096 1.30E−47 Petuniaaxillaris An2 truncated protein. 137 G663 gi13346178 2.30E−41 Gossypiumhirsutum BNLGHi233. 137 G663 gi1101770 8.40E−41 Picea mariana MYB-liketranscriptional factor MBF1. 137 G663 gi22535556 1.20E−39 Oryza sativa(japonica myb-related protei cultivar-group) 137 G663 gi2605623 1.20E−39Oryza sativa OSMYB4. 137 G663 gi2343273 4.80E−39 Zea mays PLtranscription factor. 137 G663 gi4138299 4.80E−39 Oryza sativa subsp.transcriptional activator. indica 139 G664 AF336286 2.00E−89 Gossypiumhirsutum GHMYB9 (ghmyb9) mRNA, complete cds. 139 G664 LETHM27 7.00E−88Lycopersicon L. esculentum mRNA for esculentum THM27 protein 139 G664BG442984 9.00E−83 Gossypium arboreum GA_Ea0019B05f Gossypium arboreum7-10 d 139 G664 BM112753 1.00E−80 Solanum tuberosum EST560289 potatoroots Solanum tuberosum 139 G664 AY108280 5.00E−78 Zea mays PCO132931mRNA sequence. 139 G664 BF716393 2.00E−76 Glycine max saa19f01.y1Gm-c1058 Glycine max cDNA clone GEN 139 G664 BH537477 5.00E−76 Brassicaoleracea BOGIR45TF BOGI Brassica oleracea genomic 139 G664 HVMYB11.00E−75 Hordeum vulgare H. vulgare myb1 mRNA. 139 G664 AW7758931.00E−74 Medicago truncatula EST334958 DSIL Medicago truncatula cDNA 139G664 BQ855835 8.00E−73 Lactuca sativa QGB27N20.yg.ab1 QG_ABCDI lettucesalinas Lac 139 G664 gi13346194 3.50E−88 Gossypium hirsutum GHMYB9. 139G664 gi1167484 8.00E−85 Lycopersicon transcription factor. esculentum139 G664 gi82308 3.20E−74 Antirrhinum majus myb protein 308-gardensnapdragon. 139 G664 gi19072766 5.30E−73 Oryza sativa typical P-typeR2R3 Myb protein. 139 G664 gi127579 3.80E−71 Hordeum vulgare MYB-RELATEDPROTEIN HV1. 139 G664 gi227030 3.80E−71 Hordeum vulgare var. myb-relatedgene Hv1. distichum 139 G664 gi19386839 3.00E−69 Oryza sativa (japonicaputative myb-relat cultivar-group) 139 G664 gi127582 8.10E−69 Zea maysMYB-RELATED PROTEIN ZM38. 139 G664 gi23476285 2.10E−61 Gossypioideskirkii myb-like transcription factor 1. 139 G664 gi23476281 9.10E−61Gossypium raimondii myb-like transcription factor 1. 141 G674 BE0214752.00E−47 Glycine max sm59a03.y1 Gm-c1028 Glycine max cDNA clone GENO 141G674 AY104558 1.00E−43 Zea mays PCO116495 mRNA sequence. 141 G674BE402501 3.00E−43 Triticum aestivum CSB008F03F990908 ITEC CSB WheatEndosperm 141 G674 AW672062 2.00E−42 Sorghum bicolor LG1_354_G05.b1_A002Light Grown 1 (LG1) Sor 141 G674 CA002506 2.00E−42 Hordeum vulgaresubsp. HS07L12r HS Hordeum vulgare vulgare 141 G674 AW691296 3.00E−42Medicago truncatula NF040A12ST1F1000 Developing stem Medica 141 G674BM356984 2.00E−41 Triphysaria versicolor 12II-D5 Triphysaria versicolorroot- 141 G674 BQ290999 2.00E−41 Pinus taeda NXRV054_D07_F NXRV (NsfXylem Root wood Vertica 141 G674 AW626100 3.00E−40 LycopersiconEST320007 tomato radicle, esculentum 5 d post- 141 G674 BQ8023926.00E−40 Triticum monococcum WHE2825_D09_G17ZS Triticum monococcum v 141G674 gi13486737 5.20E−42 Oryza sativa putative transcription factor(myb). 141 G674 gi22093837 3.70E−41 Oryza sativa (japonica contains ESTsAU10 cultivar-group) 141 G674 gi19059 2.40E−37 Hordeum vulgare MybHv33.141 G674 gi5139802 8.10E−37 Glycine max GmMYB29A1. 141 G674 gi11674861.30E−36 Lycopersicon transcription factor. esculentum 141 G674 gi823109.30E−36 Antirrhinum majus myb protein 330-garden snapdragon. 141 G674gi13346188 3.20E−35 Gossypium hirsutum GHMYB25. 141 G674 gi222666734.00E−35 Vitis labrusca x Vitis myb-related transcription vinifera 141G674 gi6552389 1.40E−34 Nicotiana tabacum myb-related transcriptionfactor LBM4. 141 G674 gi15082210 1.70E−34 Fragaria x ananassatranscription factor MYB1. 143 G676 AF502295 1.00E−109 Cucumis sativuswerewolf (WER) mRNA, partial cds. 143 G676 BF275643 2.00E−56 Gossypiumarboreum GA_Eb0024J14f Gossypium arboreum 7-10 d 143 G676 BZ0785623.00E−47 Brassica oleracea lkz44b07.b1 B. oleracea002 Brassica olerac143 G676 AF034130 3.00E−42 Gossypium hirsutum MYB-like DNA-bindingdomain protein (Cmy 143 G676 BU830456 4.00E−42 Populus tremula x T008E08Populus apica Populus tremuloides 143 G676 AF401220 6.00E−42 Fragaria xananassa transcription factor MYB1 (MYB1) mRNA, 143 G676 AI7718372.00E−41 Lycopersicon EST252937 tomato ovary, esculentum TAMU Lycope 143G676 BE124666 4.00E−41 Medicago truncatula EST393701 GVN Medicagotruncatula cDNA 143 G676 BG881996 9.00E−41 Glycine max sae92f10.y1Gm-c1065 Glycine max cDNA clone GEN 143 G676 AF474115 2.00E−40 Zea maystypical P-type R2R3 Myb protein (Myb1) gene, parti 143 G676 gi205143711.10E−103 Cucumis sativus werewolf. 143 G676 gi1101770 4.10E−43 Piceamariana MYB-like transcriptional factor MBF1. 143 G676 gi234762912.50E−42 Gossypium raimondii myb-like transcription factor 2. 143 G676gi2921332 3.20E−42 Gossypium hirsutum MYB-like DNA-binding domainprotein. 143 G676 gi23476293 6.60E−42 Gossypium herbaceum myb-liketranscription factor 2. 143 G676 gi15082210 1.10E−41 Fragaria x ananassatranscription factor MYB1. 143 G676 gi23476297 1.40E−41 Gossypioideskirkii myb-like transcription factor 3. 143 G676 gi19072734 6.00E−41 Zeamays typical P-type R2R3 Myb protein. 143 G676 gi82308 1.20E−40Antirrhinum majus myb protein 308-garden snapdragon. 143 G676 gi11674843.30E−40 Lycopersicon transcription factor. esculentum 145 G680PVU420902 1.00E−149 Phaseolus vulgaris mRNA for LHY protein. 145 G680BH579338 8.00E−93 Brassica oleracea BOGDR44TF BOGD Brassica oleraceagenomic 145 G680 AAAA01009649 3.00E−59 Oryza sativa (indica ( )scaffold009649 cultivar-group) 145 G680 AP004460 2.00E−58 Oryza sativa(japonica ( ) chromosome 8 clo cultivar-group) 145 G680 BU8686643.00E−56 Populus balsamifera M118F07 Populus flow subsp. trichocarpa 145G680 BE331563 2.00E−54 Glycine max sp15d08.y1 Gm-c1042 Glycine max cDNAclone GENO 145 G680 BG524104 2.00E−49 Stevia rebaudiana 38-82 Steviafield grown leaf cDNA Stevia 145 G680 AW979367 2.00E−46 LycopersiconEST310415 tomato root esculentum deficiency, C 145 G680 BM3222873.00E−45 Sorghum bicolor PIC1_2_F02.b1_A002 Pathogen-infected compat 145G680 AY103618 5.00E−45 Zea mays PCO118792 mRNA sequence. 145 G680gi21213868 1.40E−144 Phaseolus vulgaris LHY protein. 145 G680 gi155286284.80E−24 Oryza sativa hypothetical protein~similar to Oryza sativa 145G680 gi18461206 1.10E−07 Oryza sativa (japonica contains ESTs AU10cultivar-group) 145 G680 gi18874263 6.60E−07 Antirrhinum majus MYB-liketranscription factor DIVARICAT 145 G680 gi12406993 1.70E−06 Hordeumvulgare MCB1 protein. 145 G680 gi12005328 3.20E−06 Hevea brasiliensisunknown. 145 G680 gi20067661 3.40E−06 Zea mays one repeat mybtranscriptional factor. 145 G680 gi6688529 1.20E−05 Lycopersicon I-boxbinding factor. esculentum 145 G680 gi19911577 0.00036 Glycine maxsyringolide-induced protein 1-3-1A. 145 G680 gi7677132 0.012 Secalecereale c-myb-like transcription factor. 147 G682 BU831849 8.00E−25Populus tremula x T026E01 Populus apica Populus tremuloides 147 G682BU872107 8.00E−25 Populus balsamifera Q039C07 Populus flow subsp.trichocarpa 147 G682 BM437313 1.00E−20 Vitis vinifera VVA017F06_54121 Anexpressed sequence tag da 147 G682 BI699876 4.00E−19 Glycine maxsag49b09.y1 Gm-c1081 Glycine max cDNA clone GEN 147 G682 BH9610281.00E−16 Brassica oleracea odj30d06.g1 B. oleracea002 Brassica olerac147 G682 AL750151 2.00E−14 Pinus pinaster AL750151 AS Pinus pinastercDNA clone AS06C1 147 G682 BJ476463 1.00E−13 Hordeum vulgare subsp.BJ476463 K. Sato vulgare unpublished 147 G682 AJ485557 1.00E−13 Hordeumvulgare AJ485557 S00011 Hordeum vulgare cDNA clone 147 G682 CA7622992.00E−13 Oryza sativa (indica BR060003B10F03.ab1 IRR cultivar-group) 147G682 CA736777 2.00E−12 Triticum aestivum wpi1s.pk008.n12 wpi1s Triticumaestivum c 147 G682 gi23476287 8.30E−12 Gossypium hirsutum myb-liketranscription factor 2. 147 G682 gi23476291 8.30E−12 Gossypium raimondiimyb-like transcription factor 2. 147 G682 gi23476293 8.30E−12 Gossypiumherbaceum myb-like transcription factor 2. 147 G682 gi23476295 8.30E−12Gossypioides kirkii myb-like transcription factor 2. 147 G682 gi150421202.20E−11 Zea luxurians CI protein. 147 G682 gi19548449 2.20E−11 Zea maysP-type R2R3 Myb protein. 147 G682 gi9954118 2.80E−11 Solanum tuberosumtuber-specific and sucrose- responsive e 147 G682 gi15042108 4.60E−11Zea mays subsp. CI protein. parviglumis 147 G682 gi15082210 1.50E−10Fragaria x ananassa transcription factor MYB1. 147 G682 gi222666691.50E−10 Vitis labrusca x Vitis myb-related transcription vinifera 149G715 BG591677 9.00E−91 Solanum tuberosum EST499519 P. infestans-challenged leaf So 149 G715 AW776719 2.00E−89 Medicago truncatulaEST335784 DSIL Medicago truncatula cDNA 149 G715 BE208917 2.00E−87Citrus x paradisi GF-FV-P3F5 Marsh grapefruit young flavedo 149 G715BQ411597 1.00E−86 Gossypium arboreum GA_Ed0041B06f Gossypium arboreum7-10 d 149 G715 BM065544 4.00E−86 Capsicum annuum KS07004F12 KS07Capsicum annuum cDNA, mRNA 149 G715 BI701620 4.00E−83 Glycine maxsai18a04.y1 Gm-c1053 Glycine max cDNA clone GEN 149 G715 BH7253542.00E−79 Brassica oleracea BOHVO37TF BO_2_3_KB Brassica oleracea gen 149G715 AW093662 6.00E−77 Lycopersicon EST286842 tomato mixed esculentumelicitor, BT 149 G715 AW399586 2.00E−67 Lycopersicon pennellii EST310086L. pennellii trichome, Cor 149 G715 AC134235 8.00E−66 Oryza sativa(japonica ( ) chromosome 3 clo cultivar-group) 149 G715 gi52572602.00E−52 Oryza sativa Similar to sequence of BAC F7G19 from Arabid 149G715 gi20804442 1.80E−20 Oryza sativa (japonica hypothetical protecultivar-group) 149 G715 gi18481626 3.70E−08 Zea mays repressor protein.149 G715 gi1778097 0.19 Pinus taeda expansin. 149 G715 gi2130105 0.44Triticum aestivum histone H2A.4-wheat. 149 G715 gi297871 0.47 Piceaabies histone H2A. 149 G715 gi5106924 0.56 Medicago truncatula putativecell wall protein. 149 G715 gi1247386 0.6 Nicotiana alata PRP2. 149 G715gi121981 0.8 Volvox carteri HISTONE H2A-III. 149 G715 gi1708102 0.8Chlamydomonas HISTONE H2A. reinhardtii 151 G720 BH650015 1.00E−68Brassica oleracea BOMOG70TF BO_2_3_KB Brassica oleracea gen 151 G720BG450227 3.00E−55 Medicago truncatula NF015E11DT1F1087 Drought Medicagotrunc 151 G720 BG642566 7.00E−50 Lycopersicon EST510760 tomatoesculentum shoot/meristem Lyc 151 G720 BG887673 3.00E−45 Solanumtuberosum EST513524 cSTD Solanum tuberosum cDNA clo 151 G720 BU8786345.00E−45 Populus balsamifera V049F07 Populus flow subsp. trichocarpa 151G720 BQ594416 4.00E−42 Beta vulgaris E012444-024-024-N22-SP6MPIZ-ADIS-024-develop 151 G720 AF318581 4.00E−41 Oryza sativa putativetranscription factor OsGLK1 (Glk1) mR 151 G720 AF318579 1.00E−39 Zeamays putative transcription factor GOLDEN 2 mRNA, compl 151 G720BU004944 5.00E−37 Lactuca sativa QGG6K14.yg.ab1 QG_EFGHJ lettuceserriola Lac 151 G720 AW618051 4.00E−34 Lycopersicon pennellii EST314101L. pennellii trichome, Cor 151 G720 gi13940496 1.20E−38 Zea maysputative transcription factor ZmGLK1. 151 G720 gi24308616 2.20E−27 Oryzasativa (japonica Putative response cultivar-group) 151 G720 gi139404982.10E−26 Oryza sativa putative transcription factor OsGLK1. 151 G720gi4519671 1.10E−08 Nicotiana tabacum transfactor. 151 G720 gi69421903.50E−08 Mesembryanthemum CDPK substrate protein 1; C crystallinum 151G720 gi5916207 1.90E−06 Chlamydomonas regulatory protein of P-reinhardtii starvat 151 G720 gi10198182 0.016 Cladrastis kentukea ENOD2.151 G720 gi100216 0.02 Lycopersicon extensin class II (clone uJ-2)-esculentum 151 G720 gi169878 0.032 Sesbania rostrata nodulin. 151 G720gi1808688 0.041 Sporobolus stapfianus hypothetical protein. 153 G736BH959523 2.00E−65 Brassica oleracea odh52c03.b1 B. oleracea002 Brassicaolerac 153 G736 BU868493 2.00E−43 Populus balsamifera M116E08 Populusflow subsp. trichocarpa 153 G736 AW648389 4.00E−38 LycopersiconEST326843 tomato esculentum germinating seedli 153 G736 CA8106544.00E−37 Vitis vinifera CA22LIO1IVF-E1 CA22LI Vitis vinifera cDNA cl 153G736 BE323614 4.00E−34 Medicago truncatula NF006A11PL1F1081 Phosphatestarved leaf 153 G736 BE474759 3.00E−29 Glycine max sp68c07.y1 Gm-c1044Glycine max cDNA clone GENO 153 G736 AP005167 7.00E−28 Oryza sativa(japonica ( ) chromosome 7 clo cultivar-group) 153 G736 AAAA010042987.00E−28 Oryza sativa (indica ( ) scaffold004298 cultivar-group) 153G736 CA753311 2.00E−27 Oryza sativa 00210011068.D09_010628229W. scfIR62266 Oryza s 153 G736 BJ471540 3.00E−27 Hordeum vulgare subsp.BJ471540 K. Sato vulgare unpublished 153 G736 gi19071625 5.30E−30 Oryzasativa (japonica putative zinc fing cultivar-group) 153 G736 gi154515536.50E−30 Oryza sativa Putative H-protein promoter binding factor-2 153G736 gi21538791 1.70E−27 Hordeum vulgare subsp. dof zinc finger protein.vulgare 153 G736 gi1669341 1.20E−26 Cucurbita maxima AOBP (ascorbateoxidase promoter-binding 153 G736 gi3929325 1.00E−22 Dendrobium grexputative DNA-binding prot Madame Thong-In 153 G736 gi3777436 1.30E−22Hordeum vulgare DNA binding protein. 153 G736 gi2393775 1.20E−21 Zeamays prolamin box binding factor. 153 G736 gi1360078 2.40E−21 Nicotianatabacum Zn finger protein. 153 G736 gi3790264 3.90E−21 Triticum aestivumPBF protein. 153 G736 gi7688355 6.40E−21 Solanum tuberosum Dof zincfinger protein. 155 G748 D45066 6.00E−91 Cucurbita maxima mRNA for AOBP(ascorbate oxidase promoter- 155 G748 BH530891 3.00E−69 Brassicaoleracea BOHIF05TR BOHI Brassica oleracea genomic 155 G748 AP0013833.00E−63 Oryza sativa genomic DNA, chromosome 1, clone: P0453A06. 155G748 AAAA01004298 1.00E−62 Oryza sativa (indica ( ) scaffold004298cultivar-group) 155 G748 AP005167 1.00E−62 Oryza sativa (japonica ( )chromosome 7 clo cultivar-group) 155 G748 CA783807 2.00E−56 Glycine maxsat57f01.y1 Gm-c1056 Glycine max cDNA clone SOY 155 G748 AC1379861.00E−48 Medicago truncatula clone mth2-7g6, WORKING DRAFT SEQUENCE, 155G748 AW029804 1.00E−46 Lycopersicon EST273059 tomato callus, esculentumTAMU Lycop 155 G748 BQ488386 3.00E−46 Beta vulgaris43-E8885-006-003-F11-T3 Sugar beet MPIZ-ADIS- 155 G748 HVU3123302.00E−41 Hordeum vulgare subsp. Hordeum vulgare partial dof vulgare 155G748 gi1669341 5.90E−89 Cucurbita maxima AOBP (ascorbate oxidasepromoter-binding 155 G748 gi7242908 1.80E−64 Oryza sativa ESTsC23582(S11122), AU056531 (S20663) corresp 155 G748 gi19071625 5.80E−59Oryza sativa (japonica putative zinc fing cultivar-group) 155 G748gi21538791 7.10E−38 Hordeum vulgare subsp. dof zinc finger protein.vulgare 155 G748 gi2393775 8.00E−30 Zea mays prolamin box bindingfactor. 155 G748 gi3929325 3.10E−28 Dendrobium grex putative DNA-bindingprot Madame Thong-In 155 G748 gi3777436 5.90E−25 Hordeum vulgare DNAbinding protein. 155 G748 gi3790264 2.40E−24 Triticum aestivum PBFprotein. 155 G748 gi7688355 3.50E−24 Solanum tuberosum Dof zinc fingerprotein. 155 G748 gi6092016 1.00E−23 Pisum sativum elicitor-responsiveDof protein ERDP. 157 G779 AAAA01003354 3.00E−37 Oryza sativa (indica () scaffold003354 cultivar-group) 157 G779 AP004462 3.00E−37 Oryza sativa(japonica ( ) chromosome 8 clo cultivar-group) 157 G779 AT0022341.00E−36 Brassica rapa subsp. AT002234 Flower bud pekinensis cDNA Br 157G779 BH775806 8.00E−36 Zea mays fzmb011f018c05f1 fzmb filtered libraryZea mays ge 157 G779 CA783614 3.00E−32 Glycine max sat50g04.y1 Gm-c1056Glycine max cDNA clone SOY 157 G779 BH650724 2.00E−30 Brassica oleraceaBOMIW43TR BO_2_3_KB Brassica oleracea gen 157 G779 BE451174 6.00E−28Lycopersicon EST402062 tomato root, esculentum plants pre-a 157 G779AP004693 6.00E−28 Oryza sativa chromosome 8 clone P0461F06, ***SEQUENCING IN 157 G779 BF263465 4.00E−23 Hordeum vulgare HV_CEa0006N02fHordeum vulgare seedling gre 157 G779 BG557011 3.00E−21 Sorghum bicolorEM1_41_E02.g1_A002 Embryo 1 (EM1) Sorghum b 157 G779 gi19571105 8.40E−28Oryza sativa (japonica hypothetical prote cultivar-group) 157 G779gi15528743 9.10E−26 Oryza sativa contains EST C74560(E31855)~unknownprotein. 157 G779 gi1086534 1.90E−07 Oryza officinalis transcriptionalactivator Ra homolog. 157 G779 gi1086536 4.40E−07 Oryza rufipogontranscriptional activator Ra homolog. 157 G779 gi527665 5.70E−07 Sorghumbicolor myc-like regulatory R gene product. 157 G779 gi1086526 9.80E−07Oryza australiensis transcriptional activator Ra homolog. 157 G779gi1086530 1.30E−06 Oryza longistaminata transcriptional activator Rahomolog 157 G779 gi527661 1.70E−06 Phyllostachys acuta myc-likeregulatory R gene product. 157 G779 gi3127045 2.20E−06 Petunia x hybridabHLH transcription factor JAF13. 157 G779 gi527655 2.90E−06 Pennisetumglaucum myc-like regulatory R gene product. 159 G789 BU866069 9.00E−47Populus tremula x S062C11 Populus imbib Populus tremuloides 159 G789BG591063 4.00E−40 Solanum tuberosum EST498905 P. infestans- challengedleaf So 159 G789 BH593748 7.00E−36 Brassica oleracea BOGES09TR BOGEBrassica oleracea genomic 159 G789 BM411362 2.00E−35 LycopersiconEST585689 tomato breaker esculentum fruit Lyco 159 G789 BF5189532.00E−34 Medicago truncatula EST456346 DSIL Medicago truncatula cDNA 159G789 BG041496 6.00E−34 Glycine max sv35a08.y1 Gm-c1057 Glycine max cDNAclone GENO 159 G789 BE598711 6.00E−30 Sorghum bicolor PI1_81_D03.b1_A002Pathogen induced 1 (PI1) 159 G789 BU574318 6.00E−30 Prunus dulcisPA_Ea0007A10f Almond developing seed Prunus 159 G789 CA008614 6.00E−30Hordeum vulgare subsp. HU11I14r HU Hordeum vulgare vulgare 159 G789BG052163 3.00E−28 Sorghum propinquum RHIZ2_6_H10.b1_A003 Rhizome2(RHIZ2) Sor 159 G789 gi23495742 5.00E−37 Oryza sativa (japonica putativephytochro cultivar-group) 159 G789 gi12957703 5.90E−26 Oryza sativaputative phytochrome interacting factor. 159 G789 gi5923912 2.70E−10Tulipa gesneriana bHLH transcription factor GBOF-1. 159 G789 gi10865386.70E−09 Oryza rufipogon transcriptional activator Rb homolog. 159 G789gi527657 1.80E−08 Pennisetum glaucum myc-like regulatory R gene product.159 G789 gi527665 6.30E−08 Sorghum bicolor myc-like regulatory R geneproduct. 159 G789 gi527661 1.00E−07 Phyllostachys acuta myc-likeregulatory R gene product. 159 G789 gi13346180 2.30E−07 Gossypiumhirsutum GHDEL61. 159 G789 gi4206118 2.70E−07 Mesembryanthemumtransporter homolog. crystallinum 159 G789 gi527663 2.80E−07 Tripsacumaustrale myc-like regulatory R gene product. 161 G801 BH690524 1.00E−100Brassica oleracea BOMFD23TR BO_2_3_KB Brassica oleracea gen 161 G801BQ401569 2.00E−59 Gossypium arboreum GA_Ed0005G12f Gossypium arboreum7-10 d 161 G801 AF411807 2.00E−59 Lycopersicon BAC clone Clemson_Idesculentum 127E11, comple 161 G801 BG647366 2.00E−56 Medicago truncatulaEST508985 HOGA Medicago truncatula cDNA 161 G801 AP004776 6.00E−55 Oryzasativa (japonica ( ) chromosome 2 clo cultivar-group) 161 G801 BQ7414514.00E−48 Glycine max saq18f10.y1 Gm-c1045 Glycine max cDNA clone SOY 161G801 BE344238 5.00E−48 Solanum tuberosum EST409400 potato stolon,Cornell Universi 161 G801 BQ791490 2.00E−38 Brassica rapa subsp. E4414Chinese cabbage pekinensis etiol 161 G801 AC114983 2.00E−37 Oryza sativachromosome 3 clone OSJNBa0032H19, *** SEQUENCI 161 G801 BF7172454.00E−37 Prunus persica Lf583 near-ripe peach fruit cDNA library Pru 161G801 gi20975251 2.40E−33 Oryza sativa (japonica transcription factcultivar-group) 161 G801 gi5731257 1.30E−30 Gossypium hirsutumauxin-induced basic helix- loop-helix t 161 G801 gi2580440 5.80E−27Oryza sativa PCF2. 161 G801 gi13649864 3.00E−06 Capillipedium teosintebranched1 protein. parviflorum 161 G801 gi13649873 3.00E−06 Bothriochloaodorata teosinte branched1 protein. 161 G801 gi21624275 6.20E−06Pueraria montana var. PlCYC1. lobata 161 G801 gi6358622 3.70E−05Digitalis purpurea cyc4 protein. 161 G801 gi6358625 3.70E−05 Misopatesorontium cyc4 protein. 161 G801 gi21624285 6.70E−05 Sophora flavescensSfCYC2. 161 G801 gi6358621 6.90E−05 Antirrhinum majus cyc4 protein.subsp. cirrhigerum 163 G849 CRO251686 1.00E−126 Catharanthus roseus mRNAfor MYB-like DNA- binding protein 163 G849 AF543195 1.00E−117 Nicotianaglutinosa telomere binding protein TBP1 mRNA, com 163 G849 HSBPF11.00E−111 Petroselinum crispum P. crispum BPF-1 mRNA. 163 G849 ZMIBP27.00E−89 Zea mays Z. mays IBP2 mRNA for initiator-binding protein. 163G849 CA815602 8.00E−69 Vitis vinifera CA12EI204IIF_C11 CabernetSauvignon Leaf-C 163 G849 BM359662 6.00E−68 Gossypium arboreumGA_Ea0022I07r Gossypium arboreum 7-10 d 163 G849 AF242298 3.00E−66 Oryzasativa telomere binding protein-1 mRNA, complete cds. 163 G849 BU8167045.00E−65 Populus tremula x N070D06 Populus bark Populus tremuloides 163G849 BH443698 2.00E−57 Brassica oleracea BOGWU55TF BOGW Brassicaoleracea genomic 163 G849 BE432238 5.00E−52 Lycopersicon EST398767tomato breaker esculentum fruit, TIG 163 G849 gi12043533 7.30E−129Catharanthus roseus MYB-like DNA-binding protein. 163 G849 gi236643573.10E−118 Nicotiana glutinosa telomere binding protein TBP1. 163 G849gi2129918 1.60E−100 Petroselinum crispum BPF-1 protein-parsley. 163 G849gi1076813 2.60E−93 Zea mays initiator-binding protein- maize. 163 G849gi9716453 4.20E−71 Oryza sativa telomere binding protein-1; TBP1. 163G849 gi20804653 0.46 Oryza sativa (japonica histone H1-like prcultivar-group) 163 G849 gi15148918 0.85 Phaseolus vulgaris homeodomainleucine zipper protein HDZ 163 G849 gi126240 0.93 Sesbania rostrataLeghemoglobin 2 (Srglb2). 163 G849 gi15723363 0.97 Musa acuminatacalmodulin-like protein. 163 G849 gi19073328 1 Sorghum bicolor typicalP-type R2R3 Myb protein. 165 G859 AY036888 4.00E−55 Brassica napusMADS-box protein (FLC1) mRNA, complete cds. 165 G859 BG544805 3.00E−37Brassica rapa subsp. E2809 Chinese cabbage pekinensis etiol 165 G859BM436799 4.00E−36 Vitis vinifera VVA010B05_53181 An expressed sequencetag da 165 G859 BG596731 7.00E−36 Solanum tuberosum EST495409 cSTSSolanum tuberosum cDNA clo 165 G859 AW219962 2.00E−35 LycopersiconEST302445 tomato root esculentum during/after 165 G859 BQ994287 2.00E−31Lactuca sativa QGF6N05.yg.ab1 QG_EFGHJ lettuce serriola Lac 165 G859BI957545 2.00E−30 Hordeum vulgare HVSMEn0010B09f Hordeum vulgare rachisEST 1 165 G859 BU875165 2.00E−30 Populus balsamifera V003A12 Populusflow subsp. trichocarpa 165 G859 BJ213269 3.00E−30 Triticum aestivumBJ213269 Y. Ogihara unpublished cDNA libr 165 G859 MDU78949 8.00E−30Malus x domestica Malus domestica MADS- box protein 3 mRNA, 165 G859gi17933450 2.70E−54 Brassica napus MADS-box protein. 165 G859 gi57779049.90E−32 Malus x domestica MADS-box protein 3. 165 G859 gi36463241.60E−31 Malus domestica MADS-box protein. 165 G859 gi9367313 2.60E−31Hordeum vulgare MADS-box protein 8. 165 G859 gi6467974 5.50E−31Dendrobium grex MADS box protein Madame Thong-In DOMADS2. 165 G859gi12002141 2.40E−30 Zea mays MADS box protein 3. 165 G859 gi134461542.40E−30 Pisum sativum MADS-box transcription factor. 165 G859 gi42042342.40E−30 Lolium temulentum MADS-box protein 2. 165 G859 gi66510332.40E−30 Capsicum annuum MADS box transcription factor MADS1. 165 G859gi1483232 4.90E−30 Betula pendula MADS5 protein. 167 G864 BH4726541.00E−105 Brassica oleracea BOHPF07TF BOHP Brassica oleracea genomic 167G864 AP004902 2.00E−44 Lotus japonicus genomic DNA, chromosome 2, clone:LjT04G24, 167 G864 BM886518 5.00E−40 Glycine max sam17f08.y1 Gm-c1068Glycine max cDNA clone SOY 167 G864 AW685524 5.00E−39 Medicagotruncatula NF031C12NR1F1000 Nodulated root Medicag 167 G864 AP0018006.00E−36 Oryza sativa genomic DNA, chromosome 1, PAC clone: P0443E05.167 G864 LEU89257 6.00E−32 Lycopersicon DNA-binding protein Pti6esculentum mRNA, comp 167 G864 AAAA01000263 7.00E−31 Oryza sativa(indica ( ) scaffold000263 cultivar-group) 167 G864 BQ873772 8.00E−30Lactuca sativa QGI2I03.yg.ab1 QG_ABCDI lettuce salinas Lact 167 G864AF058827 7.00E−29 Nicotiana tabacum TSI1 (Tsi1) mRNA, complete cds. 167G864 BZ419846 3.00E−25 Zea mays if61a07.b1 WGS-ZmaysF (DH5a methylfiltered) Zea m 167 G864 gi8096469 1.60E−38 Oryza sativa Similar toArabidopsis thaliana chromosome 4 167 G864 gi2213785 1.00E−34Lycopersicon Pti6. esculentum 167 G864 gi23617235 3.70E−25 Oryza sativa(japonica contains ESTs AU16 cultivar-group) 167 G864 gi3065895 7.60E−25Nicotiana tabacum TSI1. 167 G864 gi3264767 1.90E−21 Prunus armeniaca AP2domain containing protein. 167 G864 gi8571476 4.30E−21 Atriplexhortensis apetala2 domain-containing protein. 167 G864 gi173856362.80E−20 Matricaria chamomilla ethylene-responsive element binding 167G864 gi8809571 4.50E−20 Nicotiana sylvestris ethylene-responsive elementbinding 167 G864 gi7528276 5.70E−20 Mesembryanthemum AP2-relatedtranscription f crystallinum 167 G864 gi21908036 9.30E−20 Zea mays DREbinding factor 1. 169 G867 BQ971511 2.00E−94 Helianthus annuusQHB7E05.yg.ab1 QH_ABCDI sunflower RHA801 169 G867 AP003450 6.00E−85Oryza sativa chromosome 1 clone P0034C09, *** SEQUENCING IN 169 G867AC135925 1.00E−80 Oryza sativa (japonica ( ) chromosome 5 clocultivar-group) 169 G867 AAAA01000997 1.00E−79 Oryza sativa (indica ( )scaffold000997 cultivar-group) 169 G867 BQ405698 2.00E−77 Gossypiumarboreum GA_Ed0085H02f Gossypium arboreum 7-10 d 169 G867 BZ0155214.00E−69 Brassica oleracea oeg86a05.g1 B. oleracea002 Brassica olerac169 G867 BF520598 2.00E−66 Medicago truncatula EST458071 DSIL Medicagotruncatula cDNA 169 G867 BU994579 4.00E−64 Hordeum vulgare subsp.HM07I08r HM Hordeum vulgare vulgare 169 G867 BF424857 2.00E−62 Glycinemax su59h03.y1 Gm-c1069 Glycine max cDNA clone GENO 169 G867 BU8710821.00E−61 Populus balsamifera Q026F06 Populus flow subsp. trichocarpa 169G867 gi18565433 2.40E−85 Oryza sativa (japonica DNA-binding proteicultivar-group) 169 G867 gi12328560 2.90E−73 Oryza sativa putative DNAbinding protein RAV2. 169 G867 gi10798644 7.30E−13 Nicotiana tabacum AP2domain-containing transcription fac 169 G867 gi18266198 2.50E−10Narcissus AP-2 domain containing pseudonarcissus protein. 169 G867gi20340233 2.50E−10 Thellungiella halophila ethylene responsive elementbindi 169 G867 gi22074046 1.50E−09 Lycopersicon transcription factorJERF1. esculentum 169 G867 gi3264767 6.90E−09 Prunus armeniaca AP2domain containing protein. 169 G867 gi18496063 7.10E−09 Fagus sylvaticaethylene responsive element binding prote 169 G867 gi13173164 8.30E−09Pisum sativum APETAL2-like protein. 169 G867 gi1730475 8.70E−09 Hordeumvulgare viviparous-1. 171 G869 BH591758 7.00E−65 Brassica oleraceaBOHET60TR BOHE Brassica oleracea genomic 171 G869 BQ791746 1.00E−25Brassica rapa subsp. E3454 Chinese cabbage pekinensis etiol 171 G869BF279235 2.00E−24 Gossypium arboreum GA_Eb0037N14f Gossypium arboreum7-10 d 171 G869 AAAA01006972 2.00E−20 Oryza sativa (indica ( )scaffold006972 cultivar-group) 171 G869 AP005687 2.00E−20 Oryza sativa(japonica ( ) chromosome 9 clo cultivar-group) 171 G869 BQ4831589.00E−20 Triticum aestivum WHE3505_C09_E17ZS Wheat unstressed root c 171G869 BQ591872 2.00E−19 Beta vulgaris E012583-024-016-N20-SP6MPIZ-ADIS-024-storage 171 G869 BM731589 6.00E−19 Glycine max sal81f11.y1Gm-c1063 Glycine max cDNA clone SOY 171 G869 LEU89257 2.00E−18Lycopersicon DNA-binding protein Pti6 esculentum mRNA, comp 171 G869AP002526 6.00E−18 Oryza sativa genomic DNA, chromosome 1, PAC clone:P0504H10. 171 G869 gi2213785 3.40E−22 Lycopersicon Pti6. esculentum 171G869 gi9049421 3.10E−21 Oryza sativa ESTs AU093391(E60370), AU091593(C60458), AU09 171 G869 gi3065895 3.90E−21 Nicotiana tabacum TSI1. 171G869 gi21908036 5.00E−16 Zea mays DRE binding factor 1. 171 G869gi8571476 1.00E−15 Atriplex hortensis apetala2 domain-containingprotein. 171 G869 gi18496063 2.60E−15 Fagus sylvatica ethyleneresponsive element binding prote 171 G869 gi20340233 1.60E−14Thellungiella halophila ethylene responsive element bindi 171 G869gi20160854 1.90E−14 Oryza sativa (japonica hypothetical protecultivar-group) 171 G869 gi4099914 2.00E−14 Stylosanthes hamataethylene-responsive element binding p 171 G869 gi8809573 2.00E−14Nicotiana sylvestris ethylene-responsive element binding 173 G877LES303343 1.00E−172 Lycopersicon mRNA for hypothetical esculentumprotein (ORF 173 G877 AB063576 1.00E−168 Nicotiana tabacum NtWRKY-9 mRNAfor WRKY DNA-binding protei 173 G877 IPBSPF1P 4.00E−83 Ipomoea batatasSweet potato mRNA for SPF1 protein, complet 173 G877 AX192164 1.00E−81Triticum aestivum Sequence 11 from Patent WO0149840. 173 G877 BZ0615642.00E−79 Brassica oleracea llf03c03.b1 B. oleracea002 Brassica olerac173 G877 AX192162 1.00E−78 Glycine max Sequence 9 from Patent WO0149840.173 G877 AF439274 2.00E−75 Retama raetam WRKY-like drought- inducedprotein (WRK) mRNA, 173 G877 AF459793 2.00E−75 Oryza sativa (indica ( )WRKY transcription cultivar-group) 173 G877 OSJN00012 7.00E−75 Oryzasativa chromosome 4 clone OSJNBa0089K21, *** SEQUENC 173 G877 PCU488312.00E−71 Petroselinum crispum DNA-binding protein WRKY1 mRNA, comple 173G877 gi13620227 2.80E−165 Lycopersicon hypothetical protein. esculentum173 G877 gi14530687 4.00E−122 Nicotiana tabacum WRKY DNA-bindingprotein. 173 G877 gi4894965 3.30E−72 Avena sativa DNA-binding proteinWRKY1. 173 G877 gi7484759 4.10E−71 Cucumis sativus SP8 binding proteinhomolog-cucumber. 173 G877 gi23305051 3.70E−70 Oryza sativa (indica WRKYtranscription f cultivar-group) 173 G877 gi1159877 1.40E−69 Avena fatuaDNA-binding protein. 173 G877 gi1076685 7.40E−57 Ipomoea batatas SPF1protein-sweet potato. 173 G877 gi13236649 4.10E−53 Oryza sativa putativeDNA-binding protein. 173 G877 gi16588566 1.20E−50 Solanum dulcamarathermal hysteresis protein STHP-64. 173 G877 gi18158619 2.10E−50 Retamaraetam WRKY-like drought- induced protein. 175 G881 AB028022 4.00E−58Nicotiana tabacum wizz mRNA, complete cds. 175 G881 AF204925 4.00E−58Petroselinum crispum transcription factor WRKY4 (WRKY4) mRN 175 G881BG582712 6.00E−55 Medicago truncatula EST484458 GVN Medicago truncatulacDNA 175 G881 BI935985 8.00E−49 Lycopersicon EST555874 tomato flower,esculentum anthesis L 175 G881 BG543269 4.00E−47 Brassica rapa subsp.E0763 Chinese cabbage pekinensis etiol 175 G881 BM520933 1.00E−46Glycine max sal32c10.y1 Gm-c1059 Glycine max cDNA clone SOY 175 G881BM404915 4.00E−45 Solanum tuberosum EST579242 potato roots Solanumtuberosum 175 G881 BU812081 1.00E−44 Populus tremula x UL92TA06 Populusleaf Populus tremuloides 175 G881 AW561928 5.00E−42 Gossypium hirsutumIPPGHZ0017 Cotton fiber and embryo Lambd 175 G881 BG525752 5.00E−42Stevia rebaudiana 49-34 Stevia field grown leaf cDNA Stevia 175 G881gi6472585 1.10E−60 Nicotiana tabacum WIZZ. 175 G881 gi11493822 3.30E−59Petroselinum crispum transcription factor WRKY4. 175 G881 gi11598797.60E−44 Avena fatua DNA-binding protein. 175 G881 gi5042446 1.40E−31Oryza sativa putative WRKY DNA binding protein. 175 G881 gi201609733.80E−24 Oryza sativa (japonica hypothetical prote cultivar-group) 175G881 gi18158619 1.70E−21 Retama raetam WRKY-like drought- inducedprotein. 175 G881 gi13620227 3.50E−16 Lycopersicon hypothetical protein.esculentum 175 G881 gi1076685 4.50E−15 Ipomoea batatas SPF1protein-sweet potato. 175 G881 gi23305051 6.10E−15 Oryza sativa (indicaWRKY transcription f cultivar-group) 175 G881 gi3420906 6.70E−15Pimpinella brachycarpa zinc finger protein; WRKY1. 177 G892 AP0041258.00E−38 Oryza sativa chromosome 2 clone OJ1767_D02, *** SEQUENCING 177G892 AAAA01003485 7.00E−37 Oryza sativa (indica ( ) scaffold003485cultivar-group) 177 G892 AP004687 7.00E−37 Oryza sativa (japonica ( )chromosome 6 clo cultivar-group) 177 G892 BH494985 3.00E−36 Brassicaoleracea BOHQZ69TR BOHQ Brassica oleracea genomic 177 G892 AC1357994.00E−33 Medicago truncatula clone mth2-11f14, WORKING DRAFT SEQUENC 177G892 BE515999 3.00E−31 Triticum aestivum WHE0607_F08_L15ZA WheatABA-treated embry 177 G892 BE598018 2.00E−30 Sorghum bicolorPI1_68_F02.g1_A002 Pathogen induced 1 (PI1) 177 G892 AF411807 6.00E−30Lycopersicon BAC clone Clemson_Id esculentum 127E11, comple 177 G892BQ163187 8.00E−30 Zea mays 952045H12.y2 952-BMS tissue from Walbot Lab(red 177 G892 AV837063 8.00E−30 Hordeum vulgare subsp. AV837063 K. Satovulgare unpublished 177 G892 gi18087865 2.10E−34 Oryza sativa putativezinc finger protein. 177 G892 gi19571000 3.10E−32 Oryza sativa (japonicahypothetical prote cultivar-group) 177 G892 gi4651204 8.10E−17 Cicerarietinum ring finger protein. 177 G892 gi23386073 3.00E−15 Tulipagesneriana unnamed protein product. 177 G892 gi22597166 2.40E−08 Glycinemax RING-H2 finger protein. 177 G892 gi20340241 9.80E−08 Thellungiellahalophila putative RING zinc finger protein 177 G892 gi2894379 4.30E−06Hordeum vulgare ring finger protein. 177 G892 gi12003386 6.60E−06Nicotiana tabacum Avr9/Cf-9 rapidly elicited protein 132. 177 G892gi18092342 1.00E−05 Zea mays ring-H2 zinc finger protein. 177 G892gi6650528 1.30E−05 Oryza sativa subsp. putative transcription factojaponica 179 G896 BE412616 1.00E−116 Hordeum vulgare MCG002.A02R990625ITEC MCG Barley Leaf/Culm 179 G896 BQ863573 1.00E−104 Lactuca sativaQGC24E01.yg.ab1 QG_ABCDI lettuce salinas Lac 179 G896 BQ970528 1.00E−101Helianthus annuus QHB42F12.yg.ab1 QH_ABCDI sunflower RHA801 179 G896AW255156 4.00E−93 Mentha x piperita ML1467 peppermint glandular trichomeMent 179 G896 BG445951 2.00E−88 Gossypium arboreum GA_Ea0030C19fGossypium arboreum 7-10 d 179 G896 BQ740879 5.00E−86 Glycine maxsap88e03.y1 Gm-c1045 Glycine max cDNA clone SOY 179 G896 AW0301822.00E−83 Lycopersicon EST273437 tomato callus, esculentum TAMU Lycop 179G896 BG241113 8.00E−82 Sorghum bicolor OV1_38_D04.b1_A002 Ovary 1 (OV1)Sorghum bi 179 G896 AI727328 1.00E−79 Gossypium hirsutum BNLGHi7759Six-day Cotton fiber Gossypiu 179 G896 AAAA01012924 6.00E−77 Oryzasativa (indica ( ) scaffold012924 cultivar-group) 179 G896 gi125978891.10E−128 Oryza sativa hypothetical protein. 179 G896 gi4235430 2.80E−30Hevea brasiliensis latex-abundant protein. 179 G896 gi20804732 5.40E−28Oryza sativa (japonica putative latex-abu cultivar-group) 179 G896gi23343885 4.20E−26 Lycopersicon metacaspase 1. esculentum 179 G896gi17981380 2.30E−06 Brassica oleracea zinc finger protein LSD2. 179 G896gi13509837 5.20E−06 Zea mays unnamed protein product. 179 G896 gi219920.0076 Volvox carteri extensin. 179 G896 gi2108256 0.011 Bromheadiaextensin. finlaysoniana 179 G896 gi1076211 0.074 Chlamydomonashypothetical protein VSP-3- reinhardtii Ch 179 G896 gi1903264 0.11 Pisumsativum hypothetical protein. 181 G910 BZ003194 2.00E−57 Brassicaoleracea oef80b08.g1 B. oleracea002 Brassica olerac 181 G910 BQ8650991.00E−32 Lactuca sativa QGC28L18.yg.ab1 QG_ABCDI lettuce salinas Lac 181G910 AB001888 2.00E−29 Oryza sativa mRNA for zinc finger protein,complete cds, 181 G910 BU578283 1.00E−27 Glycine max sar50h06.y1Gm-c1074 Glycine max cDNA clone SOY 181 G910 AP005113 2.00E−25 Oryzasativa (japonica ( ) chromosome 2 clo cultivar-group) 181 G910 BE5583274.00E−25 Hordeum vulgare HV_CEb0017D19f Hordeum vulgare seedling gre 181G910 BJ209915 4.00E−25 Triticum aestivum BJ209915 Y. Ogihara unpublishedcDNA libr 181 G910 BU044949 5.00E−25 Prunus persica PP_LEa0021A05f Peachdeveloping fruit mesoca 181 G910 AAAA01003074 5.00E−25 Oryza sativa(indica ( ) scaffold003074 cultivar-group) 181 G910 BQ121038 3.00E−24Solanum tuberosum EST606614 mixed potato tissues Solanum tu 181 G910gi3618320 1.30E−39 Oryza sativa zinc finger protein. 181 G910 gi228549862.30E−14 Brassica nigra COL1 protein. 181 G910 gi23495871 5.60E−14 Oryzasativa (japonica putative zinc-fing cultivar-group) 181 G910 gi109463371.20E−12 Ipomoea nil CONSTANS-like protein. 181 G910 gi3341723 2.20E−12Raphanus sativus CONSTANS-like 1 protein. 181 G910 gi21667475 1.10E−11Hordeum vulgare CONSTANS-like protein. 181 G910 gi4091804 1.20E−11 Malusx domestica CONSTANS-like protein 1. 181 G910 gi4557093 4.50E−11 Pinusradiata zinc finger protein. 181 G910 gi2303681 6.30E−11 Brassica napusunnamed protein product. 181 G910 gi21655160 2.80E−06 Hordeum vulgaresubsp. CONSTANS-like protein vulgare CO6. 183 G911 AI352907 1.00E−50Brassica napus MB73-1H PZ204.BNlib Brassica napus cDNA clon 183 G911BG543052 7.00E−28 Brassica rapa subsp. E0523 Chinese cabbage pekinensisetiol 183 G911 BQ849490 4.00E−24 Lactuca sativa QGB10A17.yg.ab1 QG_ABCDIlettuce salinas Lac 183 G911 BU891914 1.00E−23 Populus tremula P057A07Populus petioles cDNA library Popul 183 G911 BU885427 1.00E−23 Populustremula x R031B05 Populus root Populus tremuloides 183 G911 AW0345593.00E−23 Lycopersicon EST278175 tomato callus, esculentum TAMU Lycop 183G911 BZ013045 1.00E−22 Brassica oleracea oek67d06.b1 B. oleracea002Brassica olerac 183 G911 BG269593 3.00E−22 Mesembryanthemum L0-3678T3Ice plant crystallinum Lambda Un 183 G911 AI729600 7.00E−22 Gossypiumhirsutum BNLGHi13753 Six-day Cotton fiber Gossypi 183 G911 BG7263132.00E−21 Glycine max sae08f02.y1 Gm-c1055 Glycine max cDNA clone GEN 183G911 gi20805085 7.20E−13 Oryza sativa (japonica hypothetical protecultivar-group) 183 G911 gi14164467 1.20E−12 Oryza sativa hypotheticalprotein. 183 G911 gi20340241 5.10E−12 Thellungiella halophila putativeRING zinc finger protein 183 G911 gi20152976 1.70E−11 Hordeum vulgaresubsp. similar to A. thaliana C3H vulgare 183 G911 gi17016985 5.80E−11Cucumis melo RING-H2 zinc finger protein. 183 G911 gi23451086 7.60E−11Medicago sativa RING-H2 protein. 183 G911 gi18092342 1.40E−09 Zea maysring-H2 zinc finger protein. 183 G911 gi12003386 6.80E−09 Nicotianatabacum Avr9/Cf-9 rapidly elicited protein 132. 183 G911 gi10862257.00E−09 Lotus japonicus RING-finger protein-Lotus japonicus. 183 G911gi2894379 2.20E−08 Hordeum vulgare ring finger protein. 185 G912BH498662 2.00E−93 Brassica oleracea BOGTO66TR BOGT Brassica oleraceagenomic 185 G912 AF084185 2.00E−75 Brassica napus dehydration responsiveelement binding prote 185 G912 AF211531 1.00E−59 Nicotiana tabacumAvr9/Cf-9 rapidly elicited protein 111B 185 G912 AY034473 1.00E−55Lycopersicon putative transcriptional esculentum activator 185 G912BG321601 4.00E−53 Descurainia sophia Ds01_01h03_RDs01_AAFC_ECORC_cold_stress 185 G912 AB080965 9.00E−53 Prunus aviumDREB1-like gene for dehydratiion responsive el 185 G912 BG5906594.00E−51 Solanum tuberosum EST498501 P. infestans- challenged leaf So185 G912 BG644969 1.00E−50 Medicago truncatula EST506588 KV3 Medicagotruncatula cDNA 185 G912 BU016783 2.00E−49 Helianthus annuusQHE14A02.yg.ab1 QH_EFGHJ sunflower RHA280 185 G912 BU871514 1.00E−47Populus balsamifera Q031D09 Populus flow subsp. trichocarpa 185 G912gi5616086 5.90E−73 Brassica napus dehydration responsive element bindingpro 185 G912 gi12003384 5.20E−58 Nicotiana tabacum Avr9/Cf-9 rapidlyelicited protein 111B 185 G912 gi23495458 3.90E−53 Prunus aviumdehydratiion responsive element binding prot 185 G912 gi185355802.00E−49 Lycopersicon putative transcriptional esculentum activato 185G912 gi19071243 1.30E−45 Hordeum vulgare CRT/DRE binding factor 1. 185G912 gi24474328 8.20E−44 Oryza sativa (japonica apetala2 domain-cocultivar-group) 185 G912 gi6983877 9.00E−38 Oryza sativa Similar to mRNAfor DREB1A (AB007787). 185 G912 gi17148651 3.90E−35 Secale cerealeCBF-like protein. 185 G912 gi20152903 1.40E−32 Hordeum vulgare subsp.CRT/DRE binding factor 2. vulgare 185 G912 gi17226801 2.10E−31 Triticumaestivum putative CRT/DRE-binding factor. 187 G913 AI352878 4.00E−87Brassica napus MB72-11D PZ204.BNlib Brassica napus cDNA clo 187 G913BH536782 1.00E−59 Brassica oleracea BOGCX29TR BOGC Brassica oleraceagenomic 187 G913 AW033835 2.00E−46 Lycopersicon EST277406 tomato callus,esculentum TAMU Lycop 187 G913 BQ411166 1.00E−43 Gossypium arboreumGA_Ed0037B05f Gossypium arboreum 7-10 d 187 G913 BQ165313 5.00E−43Medicago truncatula EST611182 KVKC Medicago truncatula cDNA 187 G913AP006060 5.00E−43 Oryza sativa (japonica ( ) chromosome 2 clocultivar-group) 187 G913 AAAA01000810 2.00E−42 Oryza sativa (indica ( )scaffold000810 cultivar-group) 187 G913 OSJN00128 2.00E−38 Oryza sativachromosome 4 clone OSJNBA0088I22, *** SEQUENC 187 G913 BQ976989 3.00E−31Helianthus annuus QHI23I22.yg.ab1 QH_ABCDI sunflower RHA801 187 G913BQ592028 6.00E−30 Beta vulgaris E012695-024-021-K17-SP6MPIZ-ADIS-024-develop 187 G913 gi14140155 1.60E−32 Oryza sativa putativeAP2 domain transcription factor. 187 G913 gi12003382 1.40E−30 Nicotianatabacum Avr9/Cf-9 rapidly elicited protein 111A 187 G913 gi203035701.40E−30 Oryza sativa (japonica putative transcrip cultivar-group) 187G913 gi18535580 3.80E−30 Lycopersicon putative transcriptionalesculentum activato 187 G913 gi23495460 4.40E−29 Prunus aviumdehydration responsive element binding prote 187 G913 gi5616086 6.50E−28Brassica napus dehydration responsive element binding pro 187 G913gi21908034 1.40E−25 Zea mays DRE binding factor 2. 187 G913 gi190712431.20E−21 Hordeum vulgare CRT/DRE binding factor 1. 187 G913 gi171486492.30E−17 Secale cereale CBF-like protein. 187 G913 gi8571476 2.30E−17Atriplex hortensis apetala2 domain-containing protein. 189 G922 AP004485 1.0e−999 Lotus japonicus genomic DNA, chromosome 2, clone: LjT08D14,189 G922 AP003259 1.00E−130 Oryza sativa chromosome 1 clone P0466H10,*** SEQUENCING IN 189 G922 AAAA01000374 1.00E−130 Oryza sativa (indica () scaffold000374 cultivar-group) 189 G922 BH493536 1.00E−121 Brassicaoleracea BOGXB10TR BOGX Brassica oleracea genomic 189 G922 CNS08CCP1.00E−92 Oryza sativa (japonica ( ) chromosome 12 cl cultivar-group) 189G922 BG643567 6.00E−82 Lycopersicon EST511761 tomato esculentumshoot/meristem Lyc 189 G922 BQ124898 2.00E−81 Medicago truncatulaEST610474 GLSD Medicago truncatula cDNA 189 G922 BU764181 2.00E−71Glycine max sas53f07.y1 Gm-c1023 Glycine max cDNA clone SOY 189 G922BG595716 3.00E−62 Solanum tuberosum EST494394 cSTS Solanum tuberosumcDNA clo 189 G922 AF378125 6.00E−55 Vitis vinifera GAI-like protein 1(GAI1) gene, complete cds 189 G922 gi22830925 6.30E−127 Oryza sativa(japonica putative gibberell cultivar-group) 189 G922 gi133656103.00E−57 Pisum sativum SCARECROW. 189 G922 gi13170126 5.20E−55 Brassicanapus unnamed protein product. 189 G922 gi10178637 6.30E−51 Zea maysSCARECROW. 189 G922 gi13937306 2.30E−50 Oryza sativagibberellin-insensitive protein OsGAI. 189 G922 gi18254373 9.20E−50Hordeum vulgare nuclear transcription factor SLN1. 189 G922 gi56401572.60E−49 Triticum aestivum gibberellin response modulator. 189 G922gi20257451 3.10E−49 Calycadenia GIA/RGA-like gibberellin multiglandulosaresp 189 G922 gi13620224 1.30E−46 Lycopersicon lateral suppressor.esculentum 189 G922 gi13620166 2.20E−41 Capsella rubella hypotheticalprotein. 191 G926 BU573158 1.00E−56 Prunus dulcis PA_Ea0003A12f Almonddeveloping seed Prunus 191 G926 BI310587 2.00E−55 Medicago truncatulaEST5312337 GESD Medicago truncatula cDN 191 G926 BQ624240 1.00E−47Citrus sinensis USDA-FP_01331 Ridge pineapple sweet orange 191 G926BH443554 3.00E−44 Brassica oleracea BOHGN12TR BOHG Brassica oleraceagenomic 191 G926 BNU33884 2.00E−39 Brassica napus clone bncbf-b1 CCAAT-binding factor B subuni 191 G926 BF113081 8.00E−38 LycopersiconEST440591 tomato breaker esculentum fruit Lyco 191 G926 BG8864942.00E−36 Solanum tuberosum EST512345 cSTD Solanum tuberosum cDNA clo 191G926 AW472517 3.00E−36 Glycine max si26c12.y1 Gm-r1030 Glycine max cDNAclone GENO 191 G926 BQ407583 6.00E−36 Gossypium arboreum GA_Ed0108F07fGossypium arboreum 7-10 d 191 G926 BG343051 7.00E−34 Hordeum vulgareHVSMEg0001N16f Hordeum vulgare pre- anthesis 191 G926 gi1173616 9.70E−41Brassica napus CCAAT-binding factor B subunit homolog. 191 G926gi2826786 1.10E−27 Oryza sativa RAPB protein. 191 G926 gi71412435.80E−27 Vitis riparia transcription factor. 191 G926 gi4731314 4.00E−19Nicotiana tabacum CCAAT-binding transcription factor subu 191 G926gi2104675 0.0061 Vicia faba transcription factor. 191 G926 gi216674710.64 Hordeum vulgare CONSTANS-like protein. 191 G926 gi13775107 0.67Phaseolus vulgaris bZIP transcription factor 2. 191 G926 gi1096930 0.69Solanum tuberosum H ATPase inhibitor. 191 G926 gi24413952 0.72 Oryzasativa (japonica putative iron supe cultivar-group) 191 G926 gi18395930.78 Zea mays heat shock protein 70 homolog {clone CHEM 3} [Ze 193 G961BU879250 2.00E−81 Populus balsamifera V057G12 Populus flow subsp.trichocarpa 193 G961 BE060921 3.00E−72 Hordeum vulgare HVSMEg0013N15fHordeum vulgare pre- anthesis 193 G961 BF098091 3.00E−70 LycopersiconEST428612 tomato nutrient esculentum deficient 193 G961 BU5479854.00E−69 Glycine max GM880014A10H12 Gm- r1088 Glycine max cDNA clone 193G961 BF645892 3.00E−67 Medicago truncatula NF042G10EC1F1083 Elicitedcell culture 193 G961 AP002542 2.00E−66 Oryza sativa genomic DNA,chromosome 6, PAC clone: P0679C08. 193 G961 AAAA01001925 2.00E−66 Oryzasativa (indica ( ) scaffold001925 cultivar-group) 193 G961 AP0045622.00E−64 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 193G961 BE357920 6.00E−62 Sorghum bicolor DG1_23_F03.b1_A002 Dark Grown 1(DG1) Sorgh 193 G961 BQ483881 6.00E−61 Triticum aestivumWHE3513_F08_K15ZS Wheat unstressed root c 193 G961 gi11875152 4.00E−83Oryza sativa putative NAM (no apical meristem) protein. 193 G961gi24413978 2.90E−64 Oryza sativa (japonica NAM-like protein.cultivar-group) 193 G961 gi22597158 8.60E−47 Glycine max no apicalmeristem-like protein. 193 G961 gi15148914 1.00E−45 Phaseolus vulgarisNAC domain protein NAC2. 193 G961 gi1279640 1.70E−45 Petunia x hybridaNAM. 193 G961 gi4218537 2.40E−44 Triticum sp. GRAB2 protein. 193 G961gi6732160 2.40E−44 Triticum monococcum unnamed protein product. 193 G961gi6175246 2.30E−41 Lycopersicon jasmonic acid 2. esculentum 193 G961gi14485513 1.00E−36 Solanum tuberosum putative NAC domain protein. 193G961 gi7716952 8.40E−35 Medicago truncatula NAC1. 195 G971 AF1320028.00E−54 Petunia x hybrida PHAP2B protein (Ap2B) mRNA, complete cds. 195G971 AF253970 6.00E−52 Picea abies APETALA2-related transcription factor1 (AP2L1) 195 G971 AF332215 6.00E−52 Malus x domestica transcriptionfactor AHAP2 (AHAP2) mRNA, 195 G971 AY069953 7.00E−52 Hordeum vulgareAPETALA2-like protein (AP2L1) mRNA, complet 195 G971 AF325506 3.00E−51Pisum sativum APETAL2-like protein mRNA, complete cds. 195 G971 BI9338114.00E−51 Lycopersicon EST553700 tomato flower, esculentum anthesis L 195G971 BG447926 6.00E−51 Medicago truncatula NF103H07EC1F1062 Elicitedcell culture 195 G971 BQ120583 1.00E−50 Solanum tuberosum EST606159mixed potato tissues Solanum tu 195 G971 BM892891 2.00E−50 Glycine maxsam49e02.y1 Gm-c1069 Glycine max cDNA clone SOY 195 G971 AF1341163.00E−50 Hyacinthus orientalis APETALA2 protein homolog HAP2 (HAP2) 195G971 gi21717332 6.90E−55 Malus x domestica transcription factor AHAP2.195 G971 gi1732031 1.60E−54 Zea mays AP2 DNA-binding domain protein. 195G971 gi24059986 1.80E−53 Oryza sativa (japonica putative indetermicultivar-group) 195 G971 gi5360996 4.20E−53 Hyacinthus orientalisAPETALA2 protein homolog HAP2. 195 G971 gi13173164 1.30E−51 Pisumsativum APETAL2-like protein. 195 G971 gi18476518 6.80E−51 Hordeumvulgare APETALA2-like protein. 195 G971 gi5081555 7.50E−50 Petunia xhybrida PHAP2A protein. 195 G971 gi11181612 2.00E−49 Picea abiesAPETALA2-related transcription factor 2. 195 G971 gi21069053 4.20E−22Brassica napus AP2/EREBP transcription factor BABY BOOM2. 195 G971gi21304227 6.40E−18 Oryza sativa ovule development aintegumenta-likeprotein 197 G974 BH517407 3.00E−57 Brassica oleracea BOGRR69TR BOGRBrassica oleracea genomic 197 G974 BI421315 2.00E−56 LycopersiconEST531981 tomato callus, esculentum TAMU Lycop 197 G974 AF2740335.00E−56 Atriplex hortensis apetala2 domain-containing protein mRNA, 197G974 BQ115095 3.00E−55 Solanum tuberosum EST600671 mixed potato tissuesSolanum tu 197 G974 BU046010 9.00E−55 Prunus persica PP_LEa0024O08fPeach developing fruit mesoca 197 G974 BQ742233 4.00E−51 Glycine maxsaq24d12.y1 Gm-c1045 Glycine max cDNA clone SOY 197 G974 BU8708805.00E−49 Populus balsamifera Q019E02 Populus flow subsp. trichocarpa 197G974 AAAA01000605 1.00E−47 Oryza sativa (indica ( ) scaffold000605cultivar-group) 197 G974 AP005525 2.00E−47 Oryza sativa (japonica ( )chromosome 9 clo cultivar-group) 197 G974 BU894329 2.00E−47 Populustremula x X007E05 Populus wood Populus tremuloides 197 G974 gi85714761.70E−45 Atriplex hortensis apetala2 domain-containing protein. 197 G974gi21908036 3.60E−43 Zea mays DRE binding factor 1. 197 G974 gi199201905.50E−31 Oryza sativa (japonica Putative AP2 domai cultivar-group) 197G974 gi14140155 1.00E−20 Oryza sativa putative AP2 domain transcriptionfactor. 197 G974 gi3264767 1.10E−20 Prunus armeniaca AP2 domaincontaining protein. 197 G974 gi3342211 2.20E−20 Lycopersicon Pti4.esculentum 197 G974 gi10798644 3.50E−20 Nicotiana tabacum AP2domain-containing transcription fac 197 G974 gi21304712 9.30E−20 Glycinemax ethylene-responsive element binding protein 1 197 G974 gi75282769.30E−20 Mesembryanthemum AP2-related transcription f crystallinum 197G974 gi8809571 9.30E−20 Nicotiana sylvestris ethylene-responsive elementbinding 199 G975 BH477624 1.00E−69 Brassica oleracea BOGNB10TF BOGNBrassica oleracea genomic 199 G975 CA486875 3.00E−64 Triticum aestivumWHE4337_A02_A03ZS Wheat meiotic anther cD 199 G975 BI978981 2.00E−60Rosa chinensis zD09 Old Blush petal SMART library Rosa chin 199 G975AP004869 9.00E−60 Oryza sativa (japonica ( ) chromosome 2 clocultivar-group) 199 G975 BU978490 1.00E−58 Hordeum vulgare subsp.HA13G05r HA Hordeum vulgare vulgare 199 G975 BG642554 8.00E−57Lycopersicon EST356031 tomato flower esculentum buds, anthe 199 G975BI958226 2.00E−54 Hordeum vulgare HVSMEn0013P17f Hordeum vulgare rachisEST 1 199 G975 BQ104740 1.00E−52 Rosa hybrid cultivar fc0212.e RosePetals (Fragrant Cloud) 199 G975 AW705973 3.00E−51 Glycine maxsk64c02.y1 Gm-c1016 Glycine max cDNA clone GENO 199 G975 AP0036151.00E−47 Oryza sativa chromosome 6 clone P0486H12, *** SEQUENCING IN 199G975 gi18650662 1.80E−25 Lycopersicon ethylene response factor 1.esculentum 199 G975 gi131754 2.10E−22 Lupinus polyphyllus PPLZ02PROTEIN. 199 G975 gi3065895 9.20E−20 Nicotiana tabacum TSI1. 199 G975gi8571476 9.30E−20 Atriplex hortensis apetala2 domain-containingprotein. 199 G975 gi19920190 1.90E−19 Oryza sativa (japonica PutativeAP2 domai cultivar-group) 199 G975 gi21908036 8.40E−19 Zea mays DREbinding factor 1. 199 G975 gi4099914 1.10E−18 Stylosanthes hamataethylene-responsive element binding p 199 G975 gi10567106 1.60E−18 Oryzasativa osERF3. 199 G975 gi8809573 9.60E−18 Nicotiana sylvestrisethylene-responsive element binding 199 G975 gi7528276 1.20E−17Mesembryanthemum AP2-related transcription f crystallinum 201 G979AY103852 1.00E−84 Zea mays PCO068306 mRNA sequence. 201 G979 BQ6250521.00E−79 Citrus sinensis USDA-FP_02143 Ridge pineapple sweet orange 201G979 BZ068932 2.00E−71 Brassica oleracea lki37e06.b1 B. oleracea002Brassica olerac 201 G979 AX555218 8.00E−70 Glycine max Sequence 3 fromPatent WO02059332. 201 G979 BG595910 4.00E−67 Solanum tuberosumEST494588 cSTS Solanum tuberosum cDNA clo 201 G979 BJ178045 3.00E−66Physcomitrella patens BJ178045 normalized ful subsp. patens 201 G979AX555220 1.00E−65 Oryza sativa Sequence 5 from Patent WO02059332. 201G979 AX058689 4.00E−65 Brassica napus Sequence 3 from Patent WO0075330.201 G979 AW030921 2.00E−63 Lycopersicon EST274228 tomato callus,esculentum TAMU Lycop 201 G979 BF646396 2.00E−57 Medicago truncatulaNF071F08EC1F1074 Elicited cell culture 201 G979 gi18844783 7.80E−71Oryza sativa (japonica hypothetical prote cultivar-group) 201 G979gi21069051 9.80E−64 Brassica napus AP2/EREBP transcription factor BABYBOOM1. 201 G979 gi21304225 2.60E−63 Oryza sativa aintegumenta-likeprotein. 201 G979 gi2652938 3.00E−62 Zea mays orf. 201 G979 gi111816124.40E−45 Picea abies APETALA2-related transcription factor 2. 201 G979gi13173164 6.70E−45 Pisum sativum APETAL2-like protein. 201 G979gi18476518 1.00E−43 Hordeum vulgare APETALA2-like protein. 201 G979gi21717332 2.50E−42 Malus x domestica transcription factor AHAP2. 201G979 gi5081555 1.40E−41 Petunia x hybrida PHAP2A protein. 201 G979gi5360996 8.60E−33 Hyacinthus orientalis APETALA2 protein homolog HAP2.203 G987 AC097277 1.00E−144 Oryza sativa chromosome 3 cloneOSJNBa0022C08, *** SEQUENCI 203 G987 AAAA01003633 1.00E−144 Oryza sativa(indica ( ) scaffold003633 cultivar-group) 203 G987 AC137064 1.00E−115Oryza sativa (japonica ( ) chromosome 11 cl cultivar-group) 203 G987BZ035237 1.00E−107 Brassica oleracea oeh62d03.b1 B. oleracea002 Brassicaolerac 203 G987 AY107709 1.00E−102 Zea mays PCO094187 mRNA sequence. 203G987 BQ406287 6.00E−79 Gossypium arboreum GA_Ed0092G04f Gossypiumarboreum 7-10 d 203 G987 BQ806671 4.00E−74 Triticum aestivumWHE3581_G12_N23ZS Wheat developing grains 203 G987 BQ148263 3.00E−73Medicago truncatula NF065C10FL1F1082 Developing flower Medi 203 G987BQ971271 3.00E−73 Helianthus annuus QHB6G17.yg.ab1 QH_ABCDI sunflowerRHA801 203 G987 CA813062 1.00E−72 Vitis vinifera CA48LU08IIF-F7 CA48LUVitis vinifera cDNA cl 203 G987 gi19571020 5.70E−135 Oryza sativa(japonica contains ESTs AU16 cultivar-group) 203 G987 gi147193321.70E−120 Oryza sativa putative SCARECROW gene regulator. 203 G987gi20334379 6.10E−42 Vitis vinifera GAI-like protein 1. 203 G987gi13170126 7.70E−41 Brassica napus unnamed protein product. 203 G987gi20257473 1.20E−40 Dubautia raillardioides GIA/RGA-like gibberellinresponse 203 G987 gi20257438 1.50E−40 Argyroxiphium GIA/RGA-lisandwicense subsp. macrocephalum 203 G987 gi20257428 1.50E−40 Dubautiamenziesii GIA/RGA-like gibberellin response modu 203 G987 gi202574671.50E−40 Dubautia arborea GIA/RGA-like gibberellin response modula 203G987 gi20257475 1.90E−40 Dubautia microcephala GIA/RGA-like gibberellinresponse m 203 G987 gi20257445 1.90E−40 Carlquistia muirii GIA/RGA-likegibberellin response modu 205 G988 CRU303349  1.0e−999 Capsella rubellaORF1, ORF2, ORF3, ORF4, ORF5 and ORF6 (pa 205 G988 BH594527 1.00E−114Brassica oleracea BOGWK18TF BOGW Brassica oleracea genomic 205 G988LES303345 1.00E−112 Lycopersicon lateral suppressor gene, esculentumORF1 and 205 G988 A84080 1.00E−111 Solanum tuberosum Sequence 9 fromPatent WO9846759. 205 G988 AP004191 2.00E−63 Oryza sativa (japonica ( )chromosome 2 clo cultivar-group) 205 G988 AAAA01001835 4.00E−62 Oryzasativa (indica ( ) scaffold001835 cultivar-group) 205 G988 AP0039446.00E−62 Oryza sativa chromosome 6 clone OJ1126_F05, *** SEQUENCING 205G988 AC137079 2.00E−48 Medicago truncatula clone mth2-27d17, WORKINGDRAFT SEQUENC 205 G988 AF378125 4.00E−48 Vitis vinifera GAI-like protein1 (GAI1) gene, complete cds 205 G988 AF460219 3.00E−47 Hordeum vulgarenuclear transcription factor SLN1 gene, com 205 G988 gi136201661.90E−211 Capsella rubella hypothetical protein. 205 G988 gi136202243.30E−88 Lycopersicon lateral suppressor. esculentum 205 G988 gi203343792.60E−53 Vitis vinifera GAI-like protein 1. 205 G988 gi13170126 4.20E−51Brassica napus unnamed protein product. 205 G988 gi18254373 3.70E−48Hordeum vulgare nuclear transcription factor SLN1. 205 G988 gi136034453.30E−47 Oryza sativa putative OsGAI. 205 G988 gi21901982 3.30E−47 Oryzasativa (japonica putative OsGAI. cultivar-group) 205 G988 gi202574512.90E−46 Calycadenia GIA/RGA-like gibberellin multiglandulosa resp 205G988 gi20257422 3.70E−46 Dubautia arborea GIA/RGA-like gibberellinresponse modula 205 G988 gi5640157 6.60E−46 Triticum aestivumgibberellin response modulator. 207 G1040 BH494598 2.00E−52 Brassicaoleracea BOGHF24TF BOGH Brassica oleracea genomic 207 G1040 BQ1153432.00E−47 Solanum tuberosum EST600919 mixed potato tissues Solanum tu 207G1040 BM526051 5.00E−29 Glycine max sa136d09.y1 Gm-c1059 Glycine maxcDNA clone SOY 207 G1040 CA498340 1.00E−28 Triticum aestivumWHE3242_B12_C24ZT Wheat meiotic anther cD 207 G1040 BQ280209 2.00E−28Zea mays 1091036A08.x1 1091- Immature ear with common ESTs 207 G1040BQ996658 4.00E−28 Lactuca sativa QGG13H02.yg.ab1 QG_EFGHJ lettuceserriola La 207 G1040 BI309203 1.00E−27 Medicago truncatula EST530613GPOD Medicago truncatula cDNA 207 G1040 AI163121 1.00E−26 Populustremula x A033P70U Hybrid aspen Populus tremuloides 207 G1040 AI4874051.00E−23 Lycopersicon EST245727 tomato ovary, esculentum TAMU Lycope 207G1040 AP005904 3.00E−19 Oryza sativa (japonica ( ) chromosome 9 clocultivar-group) 207 G1040 gi4519671 3.10E−18 Nicotiana tabacumtransfactor. 207 G1040 gi6942190 5.10E−16 Mesembryanthemum CDPKsubstrate protein 1; C crystallinum 207 G1040 gi23306130 5.20E−16 Oryzasativa (japonica Unknown protein. cultivar-group) 207 G1040 gi59162075.70E−11 Chlamydomonas regulatory protein of P- reinhardtii starvat 207G1040 gi11034542 8.50E−08 Oryza sativa hypothetical protein~similar toArabidopsis 207 G1040 gi14189890 9.80E−07 Zea mays response regulator 9.207 G1040 gi2346972 0.99 Petunia x hybrida ZPT2-11. 207 G1040 gi20583130.99 Eucalyptus gunnii cinnamoyl-CoA reductase. 207 G1040 gi103044060.99 Eucalyptus saligna cinnamoyl-CoA reductase. 207 G1040 gi22597156 1Glycine max nucleolar histone deacetylase HD2-P39. 209 G1047 BH9509679.00E−56 Brassica oleracea odh95h11.b1 B. oleracea002 Brassica olerac209 G1047 BU870843 4.00E−29 Populus balsamifera Q019A11 Populus flowsubsp. trichocarpa 209 G1047 BF051268 1.00E−28 Lycopersicon EST436443tomato esculentum developing/immatur 209 G1047 BM269595 1.00E−21 Glycinemax sak01g11.y1 Gm-c1074 Glycine max cDNA clone SOY 209 G1047 BI9773021.00E−11 Rosa chinensis eG09 Old Blush petal SMART library Rosa chin 209G1047 BQ519273 2.00E−11 Solanum tuberosum EST626688 Generation of a setof potato c 209 G1047 BM437317 8.00E−11 Vitis vinifera VVA017G01_54129An expressed sequence tag da 209 G1047 CA524885 3.00E−10 Capsicum annuumKS12044G09 KS12 Capsicum annuum cDNA, mRNA 209 G1047 AU294545 5.00E−10Zinnia elegans AU294545 zinnia cultured mesophyll cell equa 209 G1047AY045570 7.00E−10 Nicotiana tabacum bZIP transcription factor BZI-2mRNA, com 209 G1047 gi13430400 9.20E−13 Phaseolus vulgaris bZiptranscription factor. 209 G1047 gi16580130 1.20E−12 Nicotiana tabacumbZIP transcription factor BZI-2. 209 G1047 gi12829956 1.90E−12 Phaseolusacutifolius bZIP. 209 G1047 gi24460973 1.10E−11 Capsicum chinense bZIPtranscription factor. 209 G1047 gi9650828 1.10E−11 Petroselinum crispumcommon plant regulatory factor 7. 209 G1047 gi12039274 3.60E−11 Oryzasativa hypothetical protein. 209 G1047 gi22597162 4.60E−11 Glycine maxbZIP transcription factor ATB2. 209 G1047 gi3986151 4.80E−10 Raphanussativus rdLIP. 209 G1047 gi5901747 4.80E−10 Lycopersicon bZIPDNA-binding protein. esculentum 209 G1047 gi2244742 4.30E−09 Antirrhinummajus bZIP DNA-binding protein. 211 G1051 BG044358 3.00E−61 Glycine maxsaa27d10.y1 Gm-c1059 Glycine max cDNA clone GEN 211 G1051 BF2697521.00E−57 Gossypium arboreum GA_Eb0005I16f Gossypium arboreum 7-10 d 211G1051 AI729411 1.00E−49 Gossypium hirsutum BNLGHi13312 Six-day Cottonfiber Gossypi 211 G1051 AL372333 7.00E−49 Medicago truncatulaMtBA50C02R1 MtBA Medicago truncatula cD 211 G1051 BF051625 2.00E−47Lycopersicon EST436861 tomato esculentum developing/immatur 211 G1051BQ869540 2.00E−44 Lactuca sativa QGD6H14.yg.ab1 QG_ABCDI lettuce salinasLact 211 G1051 AV426757 3.00E−44 Lotus japonicus AV426757 Lotusjaponicus young plants (two- 211 G1051 BJ279680 7.00E−41 Triticumaestivum BJ279680 Y. Ogihara unpublished cDNA libr 211 G1051 AY1071082.00E−40 Zea mays PCO062113 mRNA sequence. 211 G1051 BE420598 8.00E−39Hordeum vulgare HWM000.E11 ITEC HWM Barley Leaf Library Hor 211 G1051gi8096589 3.80E−46 Oryza sativa Similar to Oryza sativa bZIPtranscriptional 211 G1051 gi20160758 1.40E−24 Oryza sativa (japonicahypothetical prote cultivar-group) 211 G1051 gi2921823 1.10E−18Paulownia kawakamii shoot-forming PKSF1. 211 G1051 gi8777512 7.30E−18Nicotiana tabacum bZIP transcriptional activator RSG. 211 G1051gi3425907 3.40E−16 Lycopersicon transcription factor VSF-1. esculentum211 G1051 gi4586586 4.70E−16 Cicer arietinum bZIP DNA binding protein.211 G1051 gi1060935 5.80E−09 Zea mays mLIP15. 211 G1051 gi4632129.70E−08 Coix lacryma-jobi opaque 2. 211 G1051 gi1905785 1.40E−07Glycine max G/HBF-1. 211 G1051 gi100163 4.30E−07 Petroselinum crispumlight-induced protein CPRF- 2-parsl 213 G1052 BG044358 8.00E−66 Glycinemax saa27d10.y1 Gm-c1059 Glycine max cDNA clone GEN 213 G1052 AP0020923.00E−65 Oryza sativa genomic DNA, chromosome 1, PAC clone: P0031E09.213 G1052 AAAA01012061 2.00E−64 Oryza sativa (indica ( ) scaffold012061cultivar-group) 213 G1052 BF269752 2.00E−56 Gossypium arboreumGA_Eb0005I16f Gossypium arboreum 7-10 d 213 G1052 AI729411 4.00E−52Gossypium hirsutum BNLGHi13312 Six-day Cotton fiber Gossypi 213 G1052BF051625 3.00E−50 Lycopersicon EST436861 tomato esculentumdeveloping/immatur 213 G1052 AL372333 2.00E−48 Medicago truncatulaMtBA50C02R1 MtBA Medicago truncatula cD 213 G1052 BH529222 8.00E−48Brassica oleracea BOHBA78TF BOHB Brassica oleracea genomic 213 G1052AV426757 1.00E−46 Lotus japonicus AV426757 Lotus japonicus young plants(two- 213 G1052 BQ866454 3.00E−45 Lactuca sativa QGC8A11.yg.ab1 QG_ABCDIlettuce salinas Lact 213 G1052 gi8096589 8.60E−75 Oryza sativa Similarto Oryza sativa bZIP transcriptional 213 G1052 gi20160758 6.90E−43 Oryzasativa (japonica hypothetical prote cultivar-group) 213 G1052 gi29218231.00E−18 Paulownia kawakamii shoot-forming PKSF1. 213 G1052 gi10766031.10E−17 Lycopersicon vsf-1 protein-tomato. esculentum 213 G1052gi8777512 3.80E−17 Nicotiana tabacum bZIP transcriptional activator RSG.213 G1052 gi4586586 3.70E−14 Cicer arietinum bZIP DNA binding protein.213 G1052 gi1060935 4.80E−09 Zea mays mLIP15. 213 G1052 gi19057852.50E−07 Glycine max G/HBF-1. 213 G1052 gi1076760 9.40E−07 Sorghumbicolor Opaque-2-related protein- sorghum. 213 G1052 gi9650826 9.60E−07Petroselinum crispum common plant regulatory factor 6. 215 G1062BQ990836 3.00E−88 Lactuca sativa QGF21D20.yg.ab1 QG_EFGHJ lettuceserriola La 215 G1062 BH470947 8.00E−84 Brassica oleracea BOGSV06TR BOGSBrassica oleracea genomic 215 G1062 BE040141 2.00E−83 Oryza sativaOD102H09 OD Oryza sativa cDNA 5′ similar to bh 215 G1062 CA5019206.00E−80 Triticum aestivum WHE4040_D03_H06ZT Wheat meiotic anther cD 215G1062 AW648468 1.00E−79 Lycopersicon EST326922 tomato esculentumgerminating seedli 215 G1062 BU763190 1.00E−78 Glycine max sas38f03.y1Gm-c1080 Glycine max cDNA clone SOY 215 G1062 BE602161 5.00E−70 Hordeumvulgare HVSMEh0102M15f Hordeum vulgare 5-45 DAP spi 215 G1062 BM1119845.00E−69 Solanum tuberosum EST559520 potato roots Solanum tuberosum 215G1062 AU291385 2.00E−68 Zinnia elegans AU291385 zinnia culturedmesophyll cell equa 215 G1062 BU983081 1.00E−57 Hordeum vulgare subsp.HA28H22r HA Hordeum vulgare vulgare 215 G1062 gi20161831 1.60E−81 Oryzasativa (japonica hypothetical prote cultivar-group) 215 G1062 gi101407541.40E−27 Oryza sativa hypothetical protein. 215 G1062 gi1142619 3.50E−13Phaseolus vulgaris phaseolin G-box binding protein PG1. 215 G1062gi527661 7.50E−12 Phyllostachys acuta myc-like regulatory R geneproduct. 215 G1062 gi10998404 7.50E−12 Petunia x hybrida anthocyanin 1.215 G1062 gi1420924 1.90E−11 Zea mays IN1. 215 G1062 gi527665 3.30E−11Sorghum bicolor myc-like regulatory R gene product. 215 G1062 gi10865269.10E−11 Oryza australiensis transcriptional activator Ra homolog. 215G1062 gi1086534 1.20E−10 Oryza officinalis transcriptional activator Rahomolog. 215 G1062 gi1086538 1.20E−10 Oryza rufipogon transcriptionalactivator Rb homolog. 217 G1063 BH700922 1.00E−87 Brassica oleraceaBOMMZ07TR BO_2_3_KB Brassica oleracea gen 217 G1063 BE451174 1.00E−43Lycopersicon EST402062 tomato root, esculentum plants pre-a 217 G1063AW832545 2.00E−43 Glycine max sm12e10.y1 Gm-c1027 Glycine max cDNA cloneGENO 217 G1063 AP004693 5.00E−42 Oryza sativa chromosome 8 cloneP0461F06, *** SEQUENCING IN 217 G1063 AAAA01006870 1.00E−39 Oryza sativa(indica ( ) scaffold006870 cultivar-group) 217 G1063 AP005655 1.00E−39Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 217 G1063BH775806 2.00E−36 Zea mays fzmb011f018c05f1 fzmb filtered library Zeamays ge 217 G1063 AT002234 4.00E−34 Brassica rapa subsp. AT002234 Flowerbud pekinensis cDNA Br 217 G1063 BF263465 3.00E−26 Hordeum vulgareHV_CEa0006N02f Hordeum vulgare seedling gre 217 G1063 CA015528 2.00E−25Hordeum vulgare subsp. HT14J12r HT Hordeum vulgare vulgare 217 G1063gi19571105 7.20E−29 Oryza sativa (japonica hypothetical protecultivar-group) 217 G1063 gi15528743 8.90E−27 Oryza sativa contains ESTC74560(E31855)~unknown protein. 217 G1063 gi6166283 1.70E−10 Pinus taedahelix-loop-helix protein 1A. 217 G1063 gi11045087 1.80E−09 Brassicanapus putative protein. 217 G1063 gi10998404 1.50E−08 Petunia x hybridaanthocyanin 1. 217 G1063 gi1142621 1.10E−07 Phaseolus vulgaris phaseolinG-box binding protein PG2. 217 G1063 gi166428 1.70E−07 Antirrhinum majusDEL. 217 G1063 gi527665 8.00E−07 Sorghum bicolor myc-like regulatory Rgene product. 217 G1063 gi3399777 9.40E−07 Glycine max symbioticammonium transporter; nodulin. 217 G1063 gi5923912 1.40E−06 Tulipagesneriana bHLH transcription factor GBOF-1. 219 G1064 AP005733 6.00E−68Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 219 G1064AF165924 4.00E−65 Gossypium hirsutum auxin-induced basic helix-loop-helix 219 G1064 AP003569 9.00E−59 Oryza sativa chromosome 6 cloneP0425F05, *** SEQUENCING IN 219 G1064 AAAA01000293 9.00E−59 Oryza sativa(indica ( ) scaffold000293 cultivar-group) 219 G1064 BG447197 1.00E−56Gossypium arboreum GA_Eb0041A19f Gossypium arboreum 7-10 d 219 G1064AW649873 7.00E−54 Lycopersicon EST328327 tomato esculentum germinatingseedli 219 G1064 BH652584 2.00E−48 Brassica oleracea BOMKX03TR BO_2_3_KBBrassica oleracea gen 219 G1064 AW695783 3.00E−45 Medicago truncatulaNF098G07ST1F1055 Developing stem Medica 219 G1064 AV422714 4.00E−45Lotus japonicus AV422714 Lotus japonicus young plants (two- 219 G1064BQ294210 5.00E−42 Zea mays 1091026H05.y2 1091- Immature ear with commonESTs 219 G1064 gi5731257 9.90E−64 Gossypium hirsutum auxin-induced basichelix- loop-helix t 219 G1064 gi20975251 8.60E−45 Oryza sativa (japonicatranscription fact cultivar-group) 219 G1064 gi2580440 3.00E−32 Oryzasativa PCF2. 219 G1064 gi20269127 1.70E−07 Lupinus albus TCP1 protein.219 G1064 gi12002867 1.10E−06 Lycopersicon cycloidea. esculentum 219G1064 gi7248461 0.00029 Zea mays root cap-specific protein. 219 G1064gi21624279 0.00082 Pueraria montana var. PlCYC3. lobata 219 G1064gi13649864 0.00085 Capillipedium teosinte branched1 protein. parviflorum219 G1064 gi13649873 0.0013 Bothriochloa odorata teosinte branched1protein. 219 G1064 gi7008009 0.0016 Pisum sativum PsAD1. 221 G1069BZ025139 1.00E−111 Brassica oleracea oeh63d12.g1 B. oleracea002 Brassicaolerac 221 G1069 AP004971 1.00E−93 Lotus japonicus genomic DNA,chromosome 5, clone: LjT45G21, 221 G1069 AP004020 2.00E−79 Oryza sativachromosome 2 clone OJ1119_A01, *** SEQUENCING 221 G1069 AAAA010173312.00E−70 Oryza sativa (indica ( ) scaffold017331 cultivar-group) 221G1069 BQ165495 2.00E−62 Medicago truncatula EST611364 KVKC Medicagotruncatula cDNA 221 G1069 AC135209 2.00E−61 Oryza sativa (japonica ( )chromosome 3 clo cultivar-group) 221 G1069 AW621455 4.00E−59Lycopersicon EST312253 tomato root esculentum during/after 221 G1069BM110212 4.00E−58 Solanum tuberosum EST557748 potato roots Solanumtuberosum 221 G1069 BQ785950 7.00E−58 Glycine max saq61f09.y1 Gm-c1076Glycine max cDNA clone SOY 221 G1069 BQ863249 1.00E−57 Lactuca sativaQGC23G02.yg.ab1 QG_ABCDI lettuce salinas Lac 221 G1069 gi240599792.10E−38 Oryza sativa (japonica similar to DNA-bin cultivar-group) 221G1069 gi15528814 4.50E−36 Oryza sativa hypothetical protein~similar toArabidopsis 221 G1069 gi4165183 7.60E−25 Antirrhinum majus SAP1 protein.221 G1069 gi2213534 1.20E−19 Pisum sativum DNA-binding PD1-like protein.221 G1069 gi2459999 1 Chlamydomonas tubulin Uni3. reinhardtii 221 G1069gi100872 1 Zea mays MFS18 protein-maize. 221 G1069 gi1362165 1 Hordeumvulgare hypothetical protein 2 (clone ES1A)-bar 223 G1073 AAAA010004864.00E−74 Oryza sativa (indica ( ) scaffold000486 cultivar-group) 223G1073 AP004165 4.00E−74 Oryza sativa chromosome 2 clone OJ1479_B12, ***SEQUENCING 223 G1073 AP005477 2.00E−67 Oryza sativa (japonica ( )chromosome 6 clo cultivar-group) 223 G1073 BZ412041 3.00E−65 Zea maysOGACG56TC ZM_0.7_1.5_KB Zea mays genomic clone ZMM 223 G1073 AJ5021903.00E−64 Medicago truncatula AJ502190 MTAMP Medicago truncatula cDNA 223G1073 BQ865858 4.00E−63 Lactuca sativa QGC6B08.yg.ab1 QG_ABCDI lettucesalinas Lact 223 G1073 BH975957 5.00E−63 Brassica oleracea odh67e11.g1B. oleracea002 Brassica olerac 223 G1073 BG134451 8.00E−62 LycopersiconEST467343 tomato crown esculentum gall Lycoper 223 G1073 AP0049713.00E−60 Lotus japonicus genomic DNA, chromosome 5, clone: LjT45G21, 223G1073 BM110212 7.00E−58 Solanum tuberosum EST557748 potato roots Solanumtuberosum 223 G1073 gi15528814 5.50E−38 Oryza sativa hypotheticalprotein~similar to Arabidopsis 223 G1073 gi24059979 1.30E−29 Oryzasativa (japonica similar to DNA-bin cultivar-group) 223 G1073 gi22135361.20E−21 Pisum sativum DNA-binding protein PD1. 223 G1073 gi41651835.70E−20 Antirrhinum majus SAP1 protein. 223 G1073 gi1166450 0.00059Lycopersicon Tfm5. esculentum 223 G1073 gi11545668 0.0051 ChlamydomonasCIA5. reinhardtii 223 G1073 gi4755087 0.0054 Zea mays aluminum-inducedprotein; Al-induced protein. 223 G1073 gi395147 0.0068 Nicotiana tabacumglycine-rich protein. 223 G1073 gi21068672 0.017 Cicer arietinumputative glicine-rich protein. 223 G1073 gi1346181 0.017 Sinapis albaGLYCINE-RICH RNA- BINDING PROTEIN GRP2A. 225 G1075 BH596283 1.00E−108Brassica oleracea BOGBL42TR BOGB Brassica oleracea genomic 225 G1075BQ165495 5.00E−88 Medicago truncatula EST611364 KVKC Medicago truncatulacDNA 225 G1075 AAAA01003389 3.00E−84 Oryza sativa (indica ( )scaffold003389 cultivar-group) 225 G1075 OSJN00182 3.00E−84 Oryza sativachromosome 4 clone OSJNBa0086O06, *** SEQUENC 225 G1075 BZ4120411.00E−76 Zea mays OGACG56TC ZM_0.7_1.5_KB Zea mays genomic clone ZMM 225G1075 AP005653 1.00E−68 Oryza sativa (japonica ( ) chromosome 2 clocultivar-group) 225 G1075 BQ863249 3.00E−65 Lactuca sativaQGC23G02.yg.ab1 QG_ABCDI lettuce salinas Lac 225 G1075 BM110212 2.00E−63Solanum tuberosum EST557748 potato roots Solanum tuberosum 225 G1075BQ838600 8.00E−63 Triticum aestivum WHE2912_D12_H24ZS Wheataluminum-stressed 225 G1075 AP004971 4.00E−62 Lotus japonicus genomicDNA, chromosome 5, clone: LjT45G21, 225 G1075 gi15528814 3.80E−39 Oryzasativa hypothetical protein~similar to Arabidopsis 225 G1075 gi240599796.60E−35 Oryza sativa (japonica similar to DNA-bin cultivar-group) 225G1075 gi4165183 7.30E−20 Antirrhinum majus SAP1 protein. 225 G1075gi2213534 2.50E−19 Pisum sativum DNA-binding PD1-like protein. 225 G1075gi3810890 3.70E−05 Cucumis sativus glycine-rich protein-2. 225 G1075gi7489009 0.0001 Lycopersicon glycine-rich protein (clone esculentumw10-1 225 G1075 gi4115615 0.0018 Zea mays root cap-specific glycine-rich protein. 225 G1075 gi1628463 0.004 Silene latifolia Men-4. 225G1075 gi395147 0.005 Nicotiana tabacum glycine-rich protein. 225 G1075gi121631 0.0056 Nicotiana sylvestris GLYCINE-RICH CELL WALL STRUCTURALPR 227 G1084 BH733462 5.00E−98 Brassica oleracea BOMEF84TF BO_2_3_KBBrassica oleracea gen 227 G1084 AAAA01002671 5.00E−79 Oryza sativa(indica ( ) scaffold002671 cultivar-group) 227 G1084 AP004622 5.00E−79Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 227 G1084AC135313 9.00E−78 Medicago truncatula clone mth2-7n18, WORKING DRAFTSEQUENCE 227 G1084 AF268596 7.00E−41 Oryza sativa bZIP (bZIP) mRNA,complete cds. 227 G1084 BG135778 2.00E−40 Lycopersicon EST468670 tomatocrown esculentum gall Lycoper 227 G1084 BQ875336 3.00E−39 Lactuca sativaQGI7N06.yg.ab1 QG_ABCDI lettuce salinas Lact 227 G1084 BQ470403 2.00E−35Hordeum vulgare HX02O04r HX Hordeum vulgare cDNA clone HX02 227 G1084BG651461 3.00E−33 Glycine max sad47a06.y1 Gm-c1075 Glycine max cDNAclone GEN 227 G1084 BI141172 4.00E−32 Sorghum bicolor IP1_44_A10.b1_A002Immature pannicle 1 (IP1 227 G1084 gi20146230 1.60E−34 Oryza sativa(japonica bzip-like transcri cultivar-group) 227 G1084 gi154086471.80E−31 Oryza sativa putative bZIP (leucine zipper) protein. 227 G1084gi22858664 9.00E−28 Gossypium hirsutum unknown. 227 G1084 gi136201680.00064 Capsella rubella hypothetical protein. 227 G1084 gi225501100.0017 Marsilea quadrifolia bZIP-like protein. 227 G1084 gi143298120.0074 Atropa belladonna putative nucleosome assembly protein 1. 227G1084 gi2257756 0.012 Zea mays nucleolar histone deacetylase HD2-p39.227 G1084 gi4106378 0.031 Brassica napus calcium-binding protein. 227G1084 gi14335 0.17 Chloroplast Oenothera ORF2280. odorata 227 G1084gi401496 0.17 Chloroplast Oenothera HYPOTHETICAL picensis PROTEIN (ORF229 G1089 BH602457 1.00E−103 Brassica oleracea BOGCB25TR BOGC Brassicaoleracea genomic 229 G1089 BQ979739 1.00E−90 Helianthus annuusQHI9B09.yg.ab1 QH_ABCDI sunflower RHA801 229 G1089 AAAA01000525 1.00E−79Oryza sativa (indica ( ) scaffold000525 cultivar-group) 229 G1089AP005779 1.00E−79 Oryza sativa (japonica ( ) chromosome 7 clocultivar-group) 229 G1089 AP003931 1.00E−79 Oryza sativa chromosome 7clone OJ1664_D08, *** SEQUENCING 229 G1089 AC135413 2.00E−78 Medicagotruncatula clone mth2-16n19, WORKING DRAFT SEQUENC 229 G1089 BE6599232.00E−76 Glycine max 1098 GmaxSC Glycine max cDNA, mRNA sequence. 229G1089 BJ224103 8.00E−76 Triticum aestivum BJ224103 Y. Ogiharaunpublished cDNA libr 229 G1089 BQ991309 2.00E−75 Lactuca sativaQGF22I10.yg.ab1 QG_EFGHJ lettuce serriola La 229 G1089 BU992003 1.00E−70Hordeum vulgare HD08I18r HD Hordeum vulgare cDNA clone HD08 229 G1089gi23237834 5.20E−149 Oryza sativa (japonica bZIP protein-like.cultivar-group) 229 G1089 gi15408647 3.40E−109 Oryza sativa putativebZIP (leucine zipper) protein. 229 G1089 gi22858664 5.80E−58 Gossypiumhirsutum unknown. 229 G1089 gi22550110 4.70E−18 Marsilea quadrifoliabZIP-like protein. 229 G1089 gi12018147 1.80E−07 Chlamydomonasvegetative cell wall protein reinhardtii gp 229 G1089 gi1184100 1.00E−06Nicotiana alata pistil extensin-like protein. 229 G1089 gi1002161.50E−06 Lycopersicon extensin class II (clone uJ-2)- esculentum 229G1089 gi6523547 4.10E−06 Volvox carteri f. nagariensishydroxyproline-rich glycopr 229 G1089 gi18873729 4.40E−06 Saccharumhybrid proline-rich protein. cultivar CP65-357 229 G1089 gi41063787.30E−06 Brassica napus calcium-binding protein. 231 G1134 BF0965556.00E−46 Lycopersicon EST360582 tomato nutrient esculentum deficient 231G1134 BH509718 2.00E−34 Brassica oleracea BOHGV18TF BOHG Brassicaoleracea genomic 231 G1134 BU091550 4.00E−33 Glycine max st74e07.y1Gm-c1054 Glycine max cDNA clone GENO 231 G1134 BF005956 1.00E−32Medicago truncatula EST434454 DSLC Medicago truncatula cDNA 231 G1134BU866761 3.00E−32 Populus tremula x S070E02 Populus imbib Populustremuloides 231 G1134 BM109038 1.00E−30 Solanum tuberosum EST556574potato roots Solanum tuberosum 231 G1134 BM436251 1.00E−29 Vitisvinifera VVA001A07_52085 An expressed sequence tag da 231 G1134 BQ2814043.00E−29 Triticum aestivum WHE3020_H08_P16ZS Wheat unstressed seedli 231G1134 BU029490 5.00E−29 Helianthus annuus QHJ10N22.yg.ab1 QH_EFGHJsunflower RHA280 231 G1134 BQ803551 8.00E−29 Triticum monococcumWHE2838_H09_O18ZS Triticum monococcum v 231 G1134 gi6166283 5.10E−35Pinus taeda helix-loop-helix protein 1A. 231 G1134 gi20161021 6.20E−33Oryza sativa (japonica contains ESTs AU05 cultivar-group) 231 G1134gi19401700 1.00E−29 Oryza sativa transcription factor RAU1. 231 G1134gi5923912 1.80E−11 Tulipa gesneriana bHLH transcription factor GBOF-1.231 G1134 gi1086538 2.80E−06 Oryza rufipogon transcriptional activatorRb homolog. 231 G1134 gi527657 1.30E−05 Pennisetum glaucum myc-likeregulatory R gene product. 231 G1134 gi3399777 0.00011 Glycine maxsymbiotic ammonium transporter; nodulin. 231 G1134 gi527665 0.00046Sorghum bicolor myc-like regulatory R gene product. 231 G1134 gi133461820.0013 Gossypium hirsutum GHDEL65. 231 G1134 gi100921 0.0025 Zea maysregulatory protein B-Peru- maize. 233 G1140 AF346303 1.00E−68 Ipomoeabatatas MADS box transcription factor (MADS4) mRNA, 233 G1140 AF3352372.00E−62 Petunia x hybrida MADS-box transcription factor FBP13 (FBP1 233G1140 BU837680 4.00E−62 Populus tremula x T104E08 Populus apica Populustremuloides 233 G1140 AF008651 6.00E−61 Solanum tuberosum MADStranscriptional factor (Stmads16) mR 233 G1140 AB050643 2.00E−60Magnolia praecocissima mRNA for putative MADS- domain transc 233 G1140AF060880 5.00E−58 Paulownia kawakamii MADS box protein mRNA, completecds. 233 G1140 AF144623 5.00E−58 Canavalia lineata MADS-boxtranscription factor (MADS) mRNA 233 G1140 AX403042 1.00E−57Lycopersicon Sequence 3 from Patent esculentum WO0204651. 233 G1140BU824503 2.00E−50 Populus tremula UB65DPB03 Populus tremula cambium cDNAlibr 233 G1140 AY104901 7.00E−49 Zea mays PCO106306 mRNA sequence. 233G1140 gi13448660 2.20E−66 Ipomoea batatas MADS box transcription factor.233 G1140 gi13384052 1.40E−64 Petunia x hybrida MADS-box transcriptionfactor FBP13. 233 G1140 gi2735764 5.00E−60 Solanum tuberosum MADStranscriptional factor; STMADS16. 233 G1140 gi17433048 2.10E−59Lycopersicon MADS-box JOINTLESS esculentum protein (LeMAD 233 G1140gi16549058 9.40E−59 Magnolia praecocissima putative MADS-domaintranscription 233 G1140 gi6652756 8.50E−58 Paulownia kawakamii MADS boxprotein. 233 G1140 gi7672991 2.90E−57 Canavalia lineata MADS-boxtranscription factor. 233 G1140 gi5295978 1.40E−48 Oryza sativa MADSbox-like protein. 233 G1140 gi9367234 1.10E−46 Hordeum vulgare MADS-boxprotein 1-2. 233 G1140 gi3986689 2.10E−45 Cichorium intybus MADS boxprotein. 235 G1143 BH962188 6.00E−31 Brassica oleracea odd86h08.b1 B.oleracea002 Brassica olerac 235 G1143 BI932387 2.00E−19 LycopersiconEST552276 tomato flower, esculentum 8 mm to pr 235 G1143 AU2884643.00E−14 Zinnia elegans AU288464 zinnia cultured mesophyll cell equa 235G1143 BF004604 7.00E−11 Medicago truncatula EST433102 KV1 Medicagotruncatula cDNA 235 G1143 PVU18348 2.00E−09 Phaseolus vulgaris phaseolinG-box binding protein PG1 (PG1 235 G1143 BQ505669 3.00E−09 Solanumtuberosum EST613084 Generation of a set of potato c 235 G1143 CA5020871.00E−08 Triticum aestivum WHE4042_E12_I24ZT Wheat meiotic anther cD 235G1143 BQ854856 2.00E−08 Lactuca sativa QGB24G11.yg.ab1 QG_ABCDI lettucesalinas Lac 235 G1143 BU763190 3.00E−08 Glycine max sas38f03.y1 Gm-c1080Glycine max cDNA clone SOY 235 G1143 AF260919 3.00E−08 Petunia x hybridaanthocyanin 1 (an1) mRNA, an1-V26 allele, 235 G1143 gi1142619 1.20E−11Phaseolus vulgaris phaseolin G-box binding protein PG1. 235 G1143gi6175252 1.20E−09 Lycopersicon jasmonic acid 3. esculentum 235 G1143gi7339702 1.40E−09 Oryza sativa EST AU065085(F11092) corresponds to aregion 235 G1143 gi10998404 3.30E−09 Petunia x hybrida anthocyanin 1.235 G1143 gi527655 3.80E−09 Pennisetum glaucum myc-like regulatory Rgene product. 235 G1143 gi22758263 1.50E−08 Oryza sativa (japonicaPutative bHLH tran cultivar-group) 235 G1143 gi3399777 1.90E−08 Glycinemax symbiotic ammonium transporter; nodulin. 235 G1143 gi43217628.90E−08 Zea mays transcription factor MYC7E. 235 G1143 gi133461801.70E−07 Gossypium hirsutum GHDEL61. 235 G1143 gi527665 6.50E−07 Sorghumbicolor myc-like regulatory R gene product. 237 G1146 AB081950  1.0e−999Oryza sativa (japonica ( ) OsPNH1 mRNA for cultivar-group) 237 G1146AY109385  1.0e−999 Zea mays CL857_2 mRNA sequence. 237 G1146 BF2696171.00E−149 Gossypium arboreum GA_Eb0005C21f Gossypium arboreum 7-10 d 237G1146 BI118817 1.00E−146 Oryza sativa EST205 Differentially expressedcDNA libraries 237 G1146 AAAA01000124 1.00E−145 Oryza sativa (indica ( )scaffold000124 cultivar-group) 237 G1146 BG648445 1.00E−138 Medicagotruncatula EST510064 HOGA Medicago truncatula cDNA 237 G1146 BG3515931.00E−135 Solanum tuberosum 129B03 Mature tuber lambda ZAP Solanum tu237 G1146 BU894661 1.00E−131 Populus tremula x X012H09 Populus woodPopulus tremuloides 237 G1146 BG125123 1.00E−126 Lycopersicon EST470769tomato esculentum shoot/meristem Lyc 237 G1146 BF265852 1.00E−120Hordeum vulgare HV_CEa0013I03f Hordeum vulgare seedling gre 237 G1146gi21280321  1.0e−999 Oryza sativa (japonica ZLL/PNH homologouscultivar-group) 237 G1146 gi6539559 1.70E−103 Oryza sativa ESTsAU068544(C30430), C98487 (E0325), D23445(C 237 G1146 gi18542175 1.20E−54Zea mays putative pinhead protein. 237 G1146 gi559557 0.02 Pyruscommunis arabinogalactan-protein. 237 G1146 gi4103618 0.59 Fragaria xananassa HyPRP. 237 G1146 gi6651027 0.66 Brassica napus high mobilitygroup protein I/Y. 237 G1146 gi322757 0.86 Nicotiana tabacum pistilextensin-like protein (clone pMG 237 G1146 gi806720 0.86 Nicotiana alataarabinogalactan-protein precursor. 237 G1146 gi1076211 0.93Chlamydomonas hypothetical protein VSP-3- reinhardtii Ch 237 G1146gi6523547 0.94 Volvox carteri f. nagariensis hydroxyproline-rich glycopr239 G1196 AX041006 1.00E−112 Zea mays Sequence 1 from Patent WO0065037.239 G1196 AX351139 1.00E−106 Oryza sativa Sequence 13 from PatentWO0166755. 239 G1196 AX049431 1.00E−105 Triticum aestivum Sequence 6from Patent WO0070069. 239 G1196 BH483537 7.00E−90 Brassica oleraceaBOGXP26TF BOGX Brassica oleracea genomic 239 G1196 AF480488 5.00E−78Nicotiana tabacum NPR1 mRNA, complete cds. 239 G1196 AAAA010000431.00E−68 Oryza sativa (indica ( ) scaffold000043 cultivar-group) 239G1196 BM111027 6.00E−68 Solanum tuberosum EST558563 potato roots Solanumtuberosum 239 G1196 BQ849921 1.00E−67 Lactuca sativa QGB11C22.yg.ab1QG_ABCDI lettuce salinas Lac 239 G1196 AF527176 9.00E−67 Brassica napusputative NPR1 (NPR1) mRNA, complete cds. 239 G1196 BQ148533 2.00E−65Medicago truncatula NF069A11FL1F1085 Developing flower Medi 239 G1196gi11340603 3.10E−118 Zea mays unnamed protein product. 239 G1196gi22535593 3.50E−111 Oryza sativa (japonica putative Regulatorcultivar-group) 239 G1196 gi18616497 3.50E−109 Triticum aestivum unnamedprotein product. 239 G1196 gi18616493 1.10E−105 Oryza sativa unnamedprotein product. 239 G1196 gi21552981 3.40E−77 Nicotiana tabacum NPR1.239 G1196 gi22003730 3.30E−71 Brassica napus putative NPR1. 239 G1196gi4433618 0.1 Dendrobium grex putative myosin heavy cha Madame Thong-In239 G1196 gi17645766 0.71 Glycine max unnamed protein product. 239 G1196gi421970 0.76 Helianthus annuus hypothetical protein 708- common sunfl239 G1196 gi223934 0.9 Hordeum vulgare var. protein, acyl carrier.distichum 241 G1198 AF036949 1.00E−119 Zea mays basic leucine zipperprotein (liguleless2) mRNA, c 241 G1198 BD016868 1.00E−100 Oryza sativaRice-origin information transmission-related g 241 G1198 NTU902141.00E−100 Nicotiana tabacum leucine zipper transcription factor TGA2.241 G1198 AF402608 1.00E−99 Phaseolus vulgaris TGA-type basic leucinezipper protein TG 241 G1198 AX180962 9.00E−99 Physcomitrella patensSequence 13 from Patent WO0145493. 241 G1198 WHTHBP1BC1 5.00E−96Triticum aestivum mRNA for transcription factor HBP-1b(c1 241 G1198VFACREBL 1.00E−90 Vicia faba CREB-like protein mRNA, complete cds. 241G1198 SOYSTGA 2.00E−84 Glycine max TGACG-motif binding protein (STGA1)mRNA, compl 241 G1198 BG645576 3.00E−82 Medicago truncatula EST507195KV3 Medicago truncatula cDNA 241 G1198 NICTGA1A 3.00E−76 Nicotiana sp.Tobacco mRNA for TGA1a DNA-binding protein. 241 G1198 gi28653944.20E−115 Zea mays basic leucine zipper protein. 241 G1198 gi201616425.40E−96 Oryza sativa (japonica putative basic leu cultivar-group) 241G1198 gi17025918 9.80E−96 Oryza sativa bZIP transcription factor. 241G1198 gi12230709 3.30E−95 Nicotiana tabacum TGACG-SEQUENCE SPECIFICDNA-BINDING PRO 241 G1198 gi15148924 4.20E−95 Phaseolus vulgarisTGA-type basic leucine zipper protein 241 G1198 gi1076782 1.00E−91Triticum aestivum transcription factor HBP- 1b(c1)-wheat 241 G1198gi7488719 1.60E−81 Glycine max transcription factor STGA1- soybean. 241G1198 gi19680 6.60E−74 Nicotiana sp. TGA1a protein (AA 1-359). 241 G1198gi100099 1.10E−73 Vicia faba DNA-binding protein VBP1- fava bean. 241G1198 gi13195751 3.30E−72 Solanum tuberosum mas-binding factor MBF3. 243G1225 BQ995023 4.00E−63 Lactuca sativa QGF8N12.yg.ab1 QG_EFGHJ lettuceserriola Lac 243 G1225 BH683493 7.00E−49 Brassica oleracea BOMIX45TFBO_2_3_KB Brassica oleracea gen 243 G1225 BI677665 3.00E−40 Robiniapseudoacacia CLS342 CLS (Cambium and bark region of 243 G1225 CA8030222.00E−39 Glycine max sau46b03.y1 Gm-c1071 Glycine max cDNA clone SOY 243G1225 BG590086 1.00E−34 Solanum tuberosum EST497928 P. infestans-challenged leaf So 243 G1225 AP004213 7.00E−31 Oryza sativa (japonica () chromosome 8 clo cultivar-group) 243 G1225 BI310616 9.00E−31 Medicagotruncatula EST5312366 GESD Medicago truncatula cDN 243 G1225 CAR0110131.00E−30 Cicer arietinum epicotyl EST, clone Can133. 243 G1225AAAA01002332 2.00E−29 Oryza sativa (indica ( ) scaffold002332cultivar-group) 243 G1225 AC098836 9.00E−29 Oryza sativa chromosome 5clone OJ2097B11, *** SEQUENCING I 243 G1225 gi24756878 4.50E−43 Oryzasativa (japonica Unknown protein. cultivar-group) 243 G1225 gi36418703.50E−20 Cicer arietinum hypothetical protein. 243 G1225 gi43217622.60E−10 Zea mays transcription factor MYC7E. 243 G1225 gi126430641.10E−09 Oryza sativa putative MYC transcription factor. 243 G1225gi1142621 2.20E−09 Phaseolus vulgaris phaseolin G-box binding proteinPG2. 243 G1225 gi527663 3.60E−08 Tripsacum australe myc-like regulatoryR gene product. 243 G1225 gi527653 2.70E−07 Pennisetum glaucum myc-likeregulatory R gene product. 243 G1225 gi1086526 3.40E−07 Oryzaaustraliensis transcriptional activator Ra homolog. 243 G1225 gi10865284.80E−07 Oryza eichingeri transcriptional activator Ra homolog. 243G1225 gi10998404 6.40E−07 Petunia x hybrida anthocyanin 1. 245 G1226BH589494 1.00E−56 Brassica oleracea BOGIA17TR BOGI Brassica oleraceagenomic 245 G1226 BQ995023 1.00E−43 Lactuca sativa QGF8N12.yg.ab1QG_EFGHJ lettuce serriola Lac 245 G1226 BI677665 5.00E−42 Robiniapseudoacacia CLS342 CLS (Cambium and bark region of 245 G1226 BE0218875.00E−36 Glycine max sm63g05.y1 Gm-c1028 Glycine max cDNA clone GENO 245G1226 AP004213 1.00E−33 Oryza sativa (japonica ( ) chromosome 8 clocultivar-group) 245 G1226 AAAA01002332 9.00E−33 Oryza sativa (indica ( )scaffold002332 cultivar-group) 245 G1226 CAR011013 6.00E−32 Cicerarietinum epicotyl EST, clone Can133. 245 G1226 BI480474 5.00E−31Triticum aestivum WHE2903_F02_L03ZS Wheat aluminum-stressed 245 G1226BG452053 6.00E−28 Medicago truncatula NF077E11LF1F1087 Developing leafMedica 245 G1226 BG590086 2.00E−27 Solanum tuberosum EST497928 P.infestans- challenged leaf So 245 G1226 gi19920107 2.20E−50 Oryza sativa(japonica Putative helix-loo cultivar-group) 245 G1226 gi36418705.30E−33 Cicer arietinum hypothetical protein. 245 G1226 gi11426214.90E−14 Phaseolus vulgaris phaseolin G-box binding protein PG2. 245G1226 gi4321762 1.10E−11 Zea mays transcription factor MYC7E. 245 G1226gi10998404 1.10E−10 Petunia x hybrida anthocyanin 1. 245 G1226 gi33997774.20E−10 Glycine max symbiotic ammonium transporter; nodulin. 245 G1226gi12643064 2.00E−09 Oryza sativa putative MYC transcription factor. 245G1226 gi6175252 5.10E−09 Lycopersicon jasmonic acid 3. esculentum 245G1226 gi4206118 3.50E−08 Mesembryanthemum transporter homolog.crystallinum 245 G1226 gi527657 5.50E−08 Pennisetum glaucum myc-likeregulatory R gene product. 247 G1229 BH473443 1.00E−96 Brassica oleraceaBOHNJ20TR BOHN Brassica oleracea genomic 247 G1229 AAAA01009795 4.00E−38Oryza sativa (indica ( ) scaffold009795 cultivar-group) 247 G1229AP005470 6.00E−38 Oryza sativa (japonica ( ) chromosome 6 clocultivar-group) 247 G1229 AP003978 7.00E−37 Oryza sativa chromosome 2clone OJ1014_E11, *** SEQUENCING 247 G1229 BG590086 2.00E−20 Solanumtuberosum EST497928 P. infestans- challenged leaf So 247 G1229 BI3106163.00E−20 Medicago truncatula EST5312366 GESD Medicago truncatula cDN 247G1229 BG316255 6.00E−20 Glycine max sab78e02.y1 Gm-c1032 Glycine maxcDNA clone GEN 247 G1229 BQ995023 4.00E−19 Lactuca sativa QGF8N12.yg.ab1QG_EFGHJ lettuce serriola Lac 247 G1229 BE033916 2.00E−18Mesembryanthemum MG02A08 MG crystallinum Mesembryanthemum c 247 G1229BU820988 8.00E−17 Populus tremula UB17CPF03 Populus tremula cambium cDNAlibr 247 G1229 gi24756878 3.30E−31 Oryza sativa (japonica Unknownprotein. cultivar-group) 247 G1229 gi3641870 2.30E−21 Cicer arietinumhypothetical protein. 247 G1229 gi1142621 1.80E−12 Phaseolus vulgarisphaseolin G-box binding protein PG2. 247 G1229 gi1420924 3.90E−11 Zeamays IN1. 247 G1229 gi12643064 4.50E−10 Oryza sativa putative MYCtranscription factor. 247 G1229 gi3399777 4.30E−09 Glycine max symbioticammonium transporter; nodulin. 247 G1229 gi10998404 1.90E−08 Petunia xhybrida anthocyanin 1. 247 G1229 gi527663 2.70E−08 Tripsacum australemyc-like regulatory R gene product. 247 G1229 gi1086526 7.20E−08 Oryzaaustraliensis transcriptional activator Ra homolog. 247 G1229 gi5276657.30E−08 Sorghum bicolor myc-like regulatory R gene product. 249 G1255BZ003641 3.00E−71 Brassica oleracea oeh85a08.g1 B. oleracea002 Brassicaolerac 249 G1255 AP004993 2.00E−67 Oryza sativa (japonica ( ) chromosome6 clo cultivar-group) 249 G1255 AAAA01023497 5.00E−45 Oryza sativa(indica ( ) scaffold023497 cultivar-group) 249 G1255 BU007090 2.00E−37Lactuca sativa QGH13F16.yg.ab1 QG_EFGHJ lettuce serriola La 249 G1255AC087181 1.00E−36 Oryza sativa chromosome 3 clone OSJNBa0018H01, ***SEQUENCI 249 G1255 BG321336 7.00E−36 Descurainia sophia Ds01_06h10_ADs01_AAFC_ECORC_cold_stress 249 G1255 BG239774 1.00E−34 Glycine maxsab74c03.y1 Gm-c1032 Glycine max cDNA clone GEN 249 G1255 BQ1390467.00E−33 Medicago truncatula NF010E05PH1F1036 Phoma-infected Medicag 249G1255 BQ489587 1.00E−31 Beta vulgaris 50-E9232-006-008-C14-T3 Sugar beetMPIZ-ADIS- 249 G1255 AI772841 7.00E−31 Lycopersicon EST253941 tomatoesculentum resistant, Cornell 249 G1255 gi13702811 7.80E−32 Oryza sativaputative zinc finger protein. 249 G1255 gi22854920 4.10E−22 Brassicanigra COL1 protein. 249 G1255 gi2895188 6.20E−21 Brassica napus CONSTANShomolog. 249 G1255 gi21667479 2.30E−19 Hordeum vulgare CONSTANS-likeprotein. 249 G1255 gi23589949 3.60E−19 Oryza sativa (japonica Hd1.cultivar-group) 249 G1255 gi4091804 4.00E−19 Malus x domesticaCONSTANS-like protein 1. 249 G1255 gi21655168 4.40E−19 Hordeum vulgaresubsp. CONSTANS-like protein vulgare CO8. 249 G1255 gi3341723 7.80E−19Raphanus sativus CONSTANS-like 1 protein. 249 G1255 gi10946337 9.40E−18Ipomoea nil CONSTANS-like protein. 249 G1255 gi4557093 6.00E−16 Pinusradiata zinc finger protein. 251 G1266 BH460596 2.00E−91 Brassicaoleracea BOGWG80TR BOGW Brassica oleracea genomic 251 G1266 AF4942011.00E−54 Lycopersicon transcription factor TSRF1 esculentum (TSRF1) 251G1266 NTU81157 2.00E−53 Nicotiana tabacum S25-XP1 DNA binding proteinmRNA, complet 251 G1266 BQ081329 8.00E−48 Glycine max san23a04.y1Gm-c1084 Glycine max cDNA clone SOY 251 G1266 BG449954 8.00E−45 Medicagotruncatula NF013A10DT1F1081 Drought Medicago trunc 251 G1266 BU8962853.00E−43 Populus tremula x X038D06 Populus wood Populus tremuloides 251G1266 AI967551 9.00E−39 Lotus japonicus Ljirnpest05-400-d11 LjirnpLambda HybriZap 251 G1266 AI055252 6.00E−36 Gossypium hirsutumcoau0003H16 Cotton Boll Abscission Zone 251 G1266 AAAA01000537 9.00E−36Oryza sativa (indica ( ) scaffold000537 cultivar-group) 251 G1266AC092263 9.00E−36 Oryza sativa chromosome 3 clone OSJNBa0033P04, ***SEQUENCI 251 G1266 gi23452024 2.10E−54 Lycopersicon transcription factorTSRF1. esculentum 251 G1266 gi1732406 1.00E−52 Nicotiana tabacum S25-XP1DNA binding protein. 251 G1266 gi19034045 8.10E−37 Oryza sativa(japonica putative DNA bindi cultivar-group) 251 G1266 gi75282764.70E−29 Mesembryanthemum AP2-related transcription f crystallinum 251G1266 gi8809571 1.20E−26 Nicotiana sylvestris ethylene-responsiveelement binding 251 G1266 gi17385636 1.80E−25 Matricaria chamomillaethylene-responsive element binding 251 G1266 gi8346775 1.00E−23Catharanthus roseus AP2-domain DNA-binding protein. 251 G1266 gi141401412.30E−23 Oryza sativa putative AP2-related transcription factor. 251G1266 gi21304712 1.30E−20 Glycine max ethylene-responsive elementbinding protein 1 251 G1266 gi24817250 4.30E−18 Cicer arietinumtranscription factor EREBP- like protein. 253 G1275 AF056948 9.00E−33Gossypium hirsutum AF056948 Cotton drought tolerant genotyp 253 G1275BQ984602 2.00E−32 Lactuca sativa QGE3d01.yg.ab1 QG_EFGHJ lettuceserriola Lac 253 G1275 BE216050 7.00E−32 Hordeum vulgare HV_CEb0009E04fHordeum vulgare seedling gre 253 G1275 AW565483 3.00E−31 Sorghum bicolorLG1_344_C03.g1_A002 Light Grown 1 (LG1) Sor 253 G1275 BM064330 4.00E−31Capsicum annuum KS01065H01 KS01 Capsicum annuum cDNA, mRNA 253 G1275BM334368 6.00E−31 Zea mays MEST136-B12.T3 ISUM5- RN Zea mays cDNA cloneMEST13 253 G1275 BG525040 6.00E−31 Stevia rebaudiana 46-57 Stevia fieldgrown leaf cDNA Stevia 253 G1275 BE230596 1.00E−30 Oryza sativa 99AS81Rice Seedling Lambda ZAPII cDNA Library 253 G1275 BF009428 2.00E−30Glycine max ss78f04.y1 Gm-c1064 Glycine max cDNA clone GENO 253 G1275BJ449458 2.00E−30 Hordeum vulgare subsp. BJ449458 K. Sato vulgareunpublished 253 G1275 gi14588677 4.80E−31 Oryza sativa hypotheticalprotein. 253 G1275 gi21644680 4.80E−31 Oryza sativa (japonicahypothetical prote cultivar-group) 253 G1275 gi4894965 6.10E−24 Avenasativa DNA-binding protein WRKY1. 253 G1275 gi14530683 2.30E−23Nicotiana tabacum WRKY DNA-binding protein. 253 G1275 gi1432056 3.80E−23Petroselinum crispum WRKY3. 253 G1275 gi18158619 5.40E−23 Retama raetamWRKY-like drought- induced protein. 253 G1275 gi24745606 7.90E−23Solanum tuberosum WRKY-type DNA binding protein. 253 G1275 gi10766853.60E−22 Ipomoea batatas SPF1 protein-sweet potato. 253 G1275 gi233050514.00E−22 Oryza sativa (indica WRKY transcription f cultivar-group) 253G1275 gi1159877 6.00E−22 Avena fatua DNA-binding protein. 255 G1305AW685439 9.00E−51 Medicago truncatula NF029D11NR1F1000 Nodulated rootMedicag 255 G1305 AB028649 6.00E−50 Nicotiana tabacum gene formyb-related transcription factor 255 G1305 PHMYBPH22 1.00E−48 Petunia xhybrida P. Hybrida myb.Ph2 gene encoding protein 255 G1305 AB0730161.00E−48 Vitis labrusca x Vitis VlmybB1-1 gene for myb- vinifera rela255 G1305 AB029160 4.00E−48 Glycine max gene for GmMYB291, complete cds.255 G1305 BQ514539 6.00E−47 Solanum tuberosum EST621954 Generation of aset of potato c 255 G1305 AW032652 8.00E−47 Lycopersicon EST276211tomato callus, esculentum TAMU Lycop 255 G1305 OSMYB1202 1.00E−46 Oryzasativa O. sativa mRNA for myb factor, 1202 bp. 255 G1305 BF2019502.00E−45 Triticum aestivum WHE1759- 1762_N04_N04ZS Wheat pre-anthesis255 G1305 AP004786 2.00E−44 Oryza sativa (japonica ( ) chromosome 2 clocultivar-group) 255 G1305 gi10140742 5.70E−51 Oryza sativa myb factor.255 G1305 gi20561 2.30E−50 Petunia x hybrida protein 2. 255 G1305gi5139814 3.70E−50 Glycine max GmMYB29B2. 255 G1305 gi6552359 2.50E−49Nicotiana tabacum myb-related transcription factor LBM1. 255 G1305gi22266673 3.70E−48 Vitis labrusca x Vitis myb-related transcriptionvinifera 255 G1305 gi127580 8.90E−47 Zea mays MYB-RELATED PROTEIN ZM1.255 G1305 gi1370140 1.80E−46 Lycopersicon myb-related transcriptionesculentum factor. 255 G1305 gi19548405 1.20E−44 Sorghum bicolor P-typeR2R3 Myb protein. 255 G1305 gi82308 8.20E−44 Antirrhinum majus mybprotein 308-garden snapdragon. 255 G1305 gi13346194 1.70E−43 Gossypiumhirsutum GHMYB9. 257 G1322 AI486576 4.00E−59 Lycopersicon EST244897tomato ovary, esculentum TAMU Lycope 257 G1322 PSMYB26 2.00E−58 Pisumsativum P. sativum mRNA for Myb- like protein (Myb26). 257 G1322BG457971 1.00E−55 Medicago truncatula NF037A10PL1F1070 Phosphate starvedleaf 257 G1322 BM528383 9.00E−54 Glycine max sa157f09.y1 Gm-c1061Glycine max cDNA clone SOY 257 G1322 BI978095 1.00E−53 Rosa chinensispE09 Old Blush petal SMART library Rosa chin 257 G1322 BQ106505 6.00E−53Rosa hybrid cultivar fc0568.e Rose Petals (Fragrant Cloud) 257 G1322BQ584246 1.00E−51 Beta vulgaris E011860-024-003-F21-SP6MPIZ-ADIS-024-inflore 257 G1322 BU867210 5.00E−48 Populus tremula xS075F04 Populus imbib Populus tremuloides 257 G1322 AB058642 6.00E−48Lilium hybrid division I LhMyb mRNA, complete cds. 257 G1322 CPU339177.00E−47 Craterostigma myb-related transcription plantagineum factor 257G1322 gi82306 2.90E−57 Antirrhinum majus myb protein 305-gardensnapdragon. 257 G1322 gi1841475 2.10E−52 Pisum sativum Myb26. 257 G1322gi1002796 5.40E−51 Craterostigma Cpm10. plantagineum 257 G1322gi13537530 4.70E−48 Lilium hybrid division I LhMyb. 257 G1322 gi131775782.00E−47 Oryza sativa Myb transcription factor JAMyb. 257 G1322gi23476307 2.90E−46 Gossypioides kirkii myb-like transcription factor 5.257 G1322 gi14249015 4.70E−46 Gossypium hirsutum myb-like transcriptionfactor Myb 5. 257 G1322 gi23476303 4.70E−46 Gossypium raimondii myb-liketranscription factor 2. 257 G1322 gi24059885 6.20E−46 Oryza sativa(japonica putative typical P cultivar-group) 257 G1322 gi190733288.00E−46 Sorghum bicolor typical P-type R2R3 Myb protein. 259 G1323BF644773 8.00E−54 Medicago truncatula NF020H12EC1F1103 Elicited cellculture 259 G1323 OSMYB1202 2.00E−53 Oryza sativa O. sativa mRNA for mybfactor, 1202 bp. 259 G1323 AP004786 3.00E−53 Oryza sativa (japonica ( )chromosome 2 clo cultivar-group) 259 G1323 AB028650 5.00E−53 Nicotianatabacum mRNA for myb-related transcription factor 259 G1323 AAAA010061264.00E−52 Oryza sativa (indica ( ) scaffold006126 cultivar-group) 259G1323 BF201950 7.00E−52 Triticum aestivum WHE1759- 1762_N04_N04ZS Wheatpre-anthesis 259 G1323 BG343209 1.00E−51 Hordeum vulgare HVSMEg0005B14fHordeum vulgare pre- anthesis 259 G1323 CA032540 1.00E−51 Hordeumvulgare subsp. HX13G05r HX Hordeum vulgare vulgare 259 G1323 PHMYBPH221.00E−51 Petunia x hybrida P. Hybrida myb.Ph2 gene encoding protein 259G1323 AB029160 2.00E−51 Glycine max gene for GmMYB291, complete cds. 259G1323 gi6552361 1.30E−52 Nicotiana tabacum myb-related transcriptionfactor LBM2. 259 G1323 gi1946265 3.50E−52 Oryza sativa myb. 259 G1323gi5139802 4.00E−51 Glycine max GmMYB29A1. 259 G1323 gi22266673 1.10E−50Vitis labrusca x Vitis myb-related transcription vinifera 259 G1323gi1370140 1.40E−50 Lycopersicon myb-related transcription esculentumfactor. 259 G1323 gi20561 2.80E−50 Petunia x hybrida protein 2. 259G1323 gi127580 9.60E−50 Zea mays MYB-RELATED PROTEIN ZM1. 259 G1323gi19548405 1.20E−49 Sorghum bicolor P-type R2R3 Myb protein. 259 G1323gi22795039 7.70E−48 Populus x canescens putative MYB transcriptionfactor. 259 G1323 gi4886264 2.30E−46 Antirrhinum majus Myb-relatedtranscription factor mixta- 261 G1330 BU867210 5.00E−76 Populus tremulax S075F04 Populus imbib Populus tremuloides 261 G1330 BQ583496 3.00E−75Beta vulgaris E011979-024-005-N01-SP6 MPIZ-ADIS-024-inflore 261 G1330AF510112 1.00E−74 Craterostigma MYB transcription factor plantagineum(MYB10) 261 G1330 AW032656 1.00E−73 Lycopersicon EST276215 tomatocallus, esculentum TAMU Lycop 261 G1330 AY026332 8.00E−71 Oryza sativaMyb transcription factor JAMyb mRNA, complete 261 G1330 AF0341332.00E−68 Gossypium hirsutum MYB-like DNA-binding domain protein (Cmy 261G1330 BJ233398 7.00E−67 Triticum aestivum BJ233398 Y. Ogiharaunpublished cDNA libr 261 G1330 BG607379 5.00E−66 Triticum monococcumWHE2471_H10_O19ZS Triticum monococcum e 261 G1330 AAAA01002218 5.00E−65Oryza sativa (indica ( ) scaffold002218 cultivar-group) 261 G1330BF325282 1.00E−64 Glycine max su20e03.y1 Gm-c1066 Glycine max cDNA cloneGENO 261 G1330 gi1002798 1.60E−70 Craterostigma Cpm5. plantagineum 261G1330 gi14249015 1.50E−69 Gossypium hirsutum myb-like transcriptionfactor Myb 5. 261 G1330 gi13177578 6.30E−69 Oryza sativa Mybtranscription factor JAMyb. 261 G1330 gi23476303 1.30E−68 Gossypiumraimondii myb-like transcription factor 2. 261 G1330 gi23476307 1.70E−68Gossypioides kirkii myb-like transcription factor 5. 261 G1330gi23476305 5.70E−68 Gossypium herbaceum myb-like transcription factor 5.261 G1330 gi19073328 1.50E−67 Sorghum bicolor typical P-type R2R3 Mybprotein. 261 G1330 gi24059885 4.60E−66 Oryza sativa (japonica putativetypical P cultivar-group) 261 G1330 gi14970950 2.60E−63 Arabis gemmiferaMYB transcription factor Atmyb2. 261 G1330 gi14970952 9.90E−54Crucihimalaya MYB transcription factor himalaica Atmyb2. 263 G1331BF644787 1.00E−65 Medicago truncatula NF016A03EC1F1020 Elicited cellculture 263 G1331 BH663145 1.00E−48 Brassica oleracea BOMIM96TRBO_2_3_KB Brassica oleracea gen 263 G1331 BE489186 4.00E−47 Triticumaestivum WHE1075_G04_M07ZS Wheat unstressed seedli 263 G1331 PSMYB261.00E−44 Pisum sativum P. sativum mRNA for Myb- like protein (Myb26).263 G1331 BM527606 5.00E−43 Glycine max sa163g06.y1 Gm-c1061 Glycine maxcDNA clone SOY 263 G1331 BU013207 6.00E−43 Lactuca sativa QGJ4A09.yg.ab1QG_EFGHJ lettuce serriola Lac 263 G1331 BU991693 2.00E−42 Hordeumvulgare HD07K18r HD Hordeum vulgare cDNA clone HD07 263 G1331 BQ4604342.00E−42 Hordeum vulgare subsp. HA09K10r HA Hordeum vulgare vulgare 263G1331 BQ106505 5.00E−42 Rosa hybrid cultivar fc0568.e Rose Petals(Fragrant Cloud) 263 G1331 AI486576 5.00E−42 Lycopersicon EST244897tomato ovary, esculentum TAMU Lycope 263 G1331 gi1841475 2.80E−43 Pisumsativum Myb26. 263 G1331 gi19073328 3.60E−43 Sorghum bicolor typicalP-type R2R3 Myb protein. 263 G1331 gi11275531 6.60E−42 Oryza sativaputative myb-related transcription factor. 263 G1331 gi82306 1.10E−41Antirrhinum majus myb protein 305-garden snapdragon. 263 G1331gi24059885 1.80E−41 Oryza sativa (japonica putative typical Pcultivar-group) 263 G1331 gi2921338 1.80E−41 Gossypium hirsutum MYB-likeDNA-binding domain protein. 263 G1331 gi1167486 2.50E−41 Lycopersicontranscription factor. esculentum 263 G1331 gi23476303 3.70E−41 Gossypiumraimondii myb-like transcription factor 2. 263 G1331 gi13537530 7.60E−41Lilium hybrid division I LhMyb. 263 G1331 gi1002796 9.70E−41Craterostigma Cpm10. plantagineum 265 G1332 AF122054 5.00E−49 Solanumtuberosum clone 9 tuber-specific and sucrose-respon 265 G1332 AW1862732.00E−41 Glycine max se65f12.y1 Gm-c1019 Glycine max cDNA clone GENO 265G1332 AF336282 2.00E−41 Gossypium hirsutum GHMYB10 (ghmyb10) mRNA,complete cds. 265 G1332 AF502295 4.00E−41 Cucumis sativus werewolf (WER)mRNA, partial cds. 265 G1332 BG441912 7.00E−41 Gossypium arboreumGA_Ea0015B19f Gossypium arboreum 7-10 d 265 G1332 BU891795 2.00E−40Populus tremula P055C08 Populus petioles cDNA library Popul 265 G1332OSC1ACTIV 3.00E−40 Oryza sativa subsp. Oryza sativa mRNA for indicatranscrip 265 G1332 AY135019 1.00E−39 Zea mays PL transcription factor(pl) mRNA, pl-W22 allele, 265 G1332 BU827658 1.00E−39 Populus tremula xK006P59P Populus apic Populus tremuloides 265 G1332 AW065119 2.00E−39Pinus taeda ST39H05 Pine TriplEx shoot tip library Pinus ta 265 G1332gi9954118 6.70E−49 Solanum tuberosum tuber-specific and sucrose-responsive e 265 G1332 gi13346186 1.40E−41 Gossypium hirsutum GHMYB10.265 G1332 gi20514371 3.70E−41 Cucumis sativus werewolf. 265 G1332gi309572 9.70E−41 Zea mays transcriptional activator. 265 G1332gi4138299 1.60E−40 Oryza sativa subsp. transcriptional activator. indica265 G1332 gi23476297 3.30E−40 Gossypioides kirkii myb-like transcriptionfactor 3. 265 G1332 gi14269333 5.40E−40 Gossypium raimondii myb-liketranscription factor Myb 3. 265 G1332 gi1101770 1.00E−38 Picea marianaMYB-like transcriptional factor MBF1. 265 G1332 gi23476293 1.60E−38Gossypium herbaceum myb-like transcription factor 2. 265 G1332gi15042120 2.10E−38 Zea luxurians CI protein. 267 G1363 BH9635851.00E−47 Brassica oleracea odd44e06.g1 B. oleracea002 Brassica olerac267 G1363 AY109469 3.00E−36 Zea mays CL724_1 mRNA sequence. 267 G1363OSRAPB 2.00E−34 Oryza sativa mRNA RAPB protein. 267 G1363 BU0835721.00E−32 Glycine max sar22h11.y1 Gm-c1049 Glycine max cDNA clone SOY 267G1363 CA794711 2.00E−32 Theobroma cacao Cac_BL_1066 Cac_BL (Bean andLeaf from Amel 267 G1363 BU987613 5.00E−30 Hordeum vulgare subsp.HF15E04r HF Hordeum vulgare vulgare 267 G1363 BU672328 8.00E−29 Triticumaestivum WHE3303_C07_F13ZS Chinese Spring wheat dr 267 G1363 BQ5071041.00E−28 Solanum tuberosum EST614519 Generation of a set of potato c 267G1363 BG457624 4.00E−28 Medicago truncatula NF104F12PL1F1101 Phosphatestarved leaf 267 G1363 BJ479271 5.00E−28 Hordeum vulgare subsp. BJ479271K. Sato unpublis spontaneum 267 G1363 gi2826786 8.10E−37 Oryza sativaRAPB protein. 267 G1363 gi7141243 8.50E−26 Vitis riparia transcriptionfactor. 267 G1363 gi4731314 7.10E−22 Nicotiana tabacum CCAAT-bindingtranscription factor subu 267 G1363 gi1173616 9.00E−22 Brassica napusCCAAT-binding factor B subunit homolog. 267 G1363 gi24414083 0.43 Oryzasativa (japonica gag-pol-like prote cultivar-group) 267 G1363 gi49025350.57 Gossypium sturtianum microsomal omega6 desaturase enzyme. 267 G1363gi15187138 0.75 Gossypium anomalum microsomal omega6 desaturase FAD2-1.267 G1363 gi4902504 0.75 Gossypium microsomal omega6 cunninghamiidesaturase enzym 267 G1363 gi4902502 0.83 Gossypium costulatummicrosomal omega6 desaturase enzyme. 267 G1363 gi4902506 0.83 Gossypiumenthyle microsomal omega6 desaturase enzyme. 269 G1411 BZ017225 3.00E−51Brassica oleracea oei67e03.b1 B. oleracea002 Brassica olerac 269 G1411BQ138607 8.00E−44 Medicago truncatula NF005C01PH1F1004 Phoma-infectedMedicag 269 G1411 BQ786702 5.00E−36 Glycine max saq72b07.y1 Gm-c1076Glycine max cDNA clone SOY 269 G1411 BM062508 7.00E−32 Capsicum annuumKS01043F09 KS01 Capsicum annuum cDNA, mRNA 269 G1411 AAAA010008322.00E−30 Oryza sativa (indica ( ) scaffold000832 cultivar-group) 269G1411 OSJN00240 2.00E−30 Oryza sativa genomic DNA, chromosome 4, BACclone: OSJNBa0 269 G1411 BE419451 2.00E−29 Triticum aestivumWWS012.C2R000101 ITEC WWS Wheat Scutellum 269 G1411 CA014817 6.00E−29Hordeum vulgare subsp. HT12H01r HT Hordeum vulgare vulgare 269 G1411BE642320 1.00E−28 Ceratopteris richardii Cri2_5_L17_SP6 CeratopterisSpore Li 269 G1411 BE494041 2.00E−27 Secale cereale WHE1277_B09_D17ZSSecale cereale anther cDNA 269 G1411 gi20160854 1.40E−29 Oryza sativa(japonica hypothetical prote cultivar-group) 269 G1411 gi141401411.50E−24 Oryza sativa putative AP2-related transcription factor. 269G1411 gi3342211 1.40E−23 Lycopersicon Pti4. esculentum 269 G1411gi10798644 2.30E−23 Nicotiana tabacum AP2 domain-containingtranscription fac 269 G1411 gi8809571 2.30E−23 Nicotiana sylvestrisethylene-responsive element binding 269 G1411 gi24817250 3.00E−23 Cicerarietinum transcription factor EREBP- like protein. 269 G1411 gi32647673.00E−23 Prunus armeniaca AP2 domain containing protein. 269 G1411gi1688233 3.80E−23 Solanum tuberosum DNA binding protein homolog. 269G1411 gi7528276 3.80E−23 Mesembryanthemum AP2-related transcription fcrystallinum 269 G1411 gi21304712 6.20E−23 Glycine maxethylene-responsive element binding protein 1 271 G1417 CA7826438.00E−58 Glycine max sat31e05.y1 Gm-c1056 Glycine max cDNA clone SOY 271G1417 AI895084 9.00E−57 Lycopersicon EST264527 tomato callus, esculentumTAMU Lycop 271 G1417 BQ625082 3.00E−56 Citrus sinensis USDA-FP_02173Ridge pineapple sweet orange 271 G1417 AC120986 2.00E−54 Oryza sativa(japonica ( ) chromosome 5 clo cultivar-group) 271 G1417 AAAA010040533.00E−54 Oryza sativa (indica ( ) scaffold004053 cultivar-group) 271G1417 BF636342 2.00E−53 Medicago truncatula NF088G12DT1F1099 DroughtMedicago trunc 271 G1417 BG838724 3.00E−50 Glycine clandestinaGc02_02f10_R Gc02_AAFC_ECORC_cold_stres 271 G1417 AU083645 2.00E−47Cryptomeria japonica AU083645 Cryptomeria japonica inner ba 271 G1417AP004967 6.00E−47 Lotus japonicus genomic DNA, chromosome 1, clone:LjT27L02, 271 G1417 BU047549 1.00E−46 Prunus persica PP_LEa0030E11fPeach developing fruit mesoca 271 G1417 gi8467950 4.80E−68 Oryza sativaSimilar to Arabidopsis thaliana chromosome 4 271 G1417 gi201609732.40E−37 Oryza sativa (japonica hypothetical prote cultivar-group) 271G1417 gi6472585 7.70E−36 Nicotiana tabacum WIZZ. 271 G1417 gi11598795.00E−35 Avena fatua DNA-binding protein. 271 G1417 gi11493822 2.50E−30Petroselinum crispum transcription factor WRKY4. 271 G1417 gi34209061.80E−21 Pimpinella brachycarpa zinc finger protein; WRKY1. 271 G1417gi4894965 4.90E−20 Avena sativa DNA-binding protein WRKY1. 271 G1417gi18158619 2.80E−19 Retama raetam WRKY-like drought- induced protein.271 G1417 gi1076685 3.60E−19 Ipomoea batatas SPF1 protein-sweet potato.271 G1417 gi13620227 1.50E−18 Lycopersicon hypothetical protein.esculentum 273 G1419 TOBBY4C 6.00E−44 Nicotiana tabacum Tobacco mRNA forEREBP-4, complete cds. 273 G1419 BU823955 5.00E−43 Populus tremulaUB58DPE07 Populus tremula cambium cDNA libr 273 G1419 AB016266 2.00E−42Nicotiana sylvestris nserf4 gene for ethylene- responsive el 273 G1419BM062245 5.00E−42 Capsicum annuum KS01040C11 KS01 Capsicum annuum cDNA,mRNA 273 G1419 AW507860 5.00E−40 Glycine max si45h05.y1 Gm-r1030 Glycinemax cDNA clone GENO 273 G1419 BG646774 5.00E−39 Medicago truncatulaEST508393 HOGA Medicago truncatula cDNA 273 G1419 AF204784 2.00E−38Lycopersicon ripening regulated protein esculentum DDTFR10/ 273 G1419BQ514195 3.00E−38 Solanum tuberosum EST621610 Generation of a set ofpotato c 273 G1419 CA812903 8.00E−35 Vitis vinifera CA48LU07IVF-D6CA48LU Vitis vinifera cDNA cl 273 G1419 BH683728 8.00E−35 Brassicaoleracea BOHTE23TR BO_2_3_KB Brassica oleracea gen 273 G1419 gi12084971.40E−48 Nicotiana tabacum EREBP-4. 273 G1419 gi8809575 9.80E−48Nicotiana sylvestris ethylene-responsive element binding 273 G1419gi12231294 3.00E−39 Lycopersicon ripening regulated protein esculentumDDTFR1 273 G1419 gi7528276 1.60E−30 Mesembryanthemum AP2-relatedtranscription f crystallinum 273 G1419 gi12597874 6.80E−30 Oryza sativaputative ethylene-responsive element binding 273 G1419 gi173856363.30E−24 Matricaria chamomilla ethylene-responsive element binding 273G1419 gi8980313 2.10E−23 Catharanthus roseus AP2-domain DNA-bindingprotein. 273 G1419 gi15623863 3.00E−23 Oryza sativa (japonica containsEST~hypot cultivar-group) 273 G1419 gi21304712 7.90E−23 Glycine maxethylene-responsive element binding protein 1 273 G1419 gi40999141.50E−21 Stylosanthes hamata ethylene-responsive element binding p 275G1449 BH939388 8.00E−43 Brassica oleracea odd83a03.g1 B. oleracea002Brassica olerac 275 G1449 BU927008 3.00E−34 Glycine max sas94e06.y1Gm-c1036 Glycine max cDNA clone SOY 275 G1449 PTR306827 5.00E−30 Populustremula x mRNA for aux/IAA pro Populus tremuloides 275 G1449 BF7279923.00E−26 Zea mays 1000057B09.x4 1000- Unigene I from Maize Genome P 275G1449 BF649039 3.00E−22 Medicago truncatula NF051G11EC1F1086 Elicitedcell culture 275 G1449 BJ228821 3.00E−20 Triticum aestivum BJ228821 Y.Ogihara unpublished cDNA libr 275 G1449 AB026823 6.00E−20 Cucumissativus CS-IAA3 mRNA, partial cds. 275 G1449 AB004933 2.00E−19 Vignaradiata mRNA for Aux22e, complete cds. 275 G1449 BU992079 2.00E−19Hordeum vulgare HD08M04r HD Hordeum vulgare cDNA clone HD08 275 G1449BU889599 3.00E−19 Populus tremula P023B06 Populus petioles cDNA libraryPopul 275 G1449 gi20269055 2.60E−31 Populus tremula x aux/IAA protein.Populus tremuloides 275 G1449 gi8096369 4.50E−27 Oryza sativa ESTsD22686(C0916), C98167(C0916) correspond 275 G1449 gi6136834 1.30E−22Cucumis sativus CS-IAA3. 275 G1449 gi4887022 7.60E−22 Nicotiana tabacumNt-iaa4.1 deduced protein. 275 G1449 gi11131105 3.90E−21 Vigna radiataAUXIN-INDUCED PROTEIN 22E (INDOLE-3- ACETIC 275 G1449 gi1352057 1.00E−20Pisum sativum AUXIN-INDUCED PROTEIN IAA4. 275 G1449 gi18071490 1.30E−20Antirrhinum majus auxin-induced AUX/IAA1. 275 G1449 gi17976835 1.20E−19Pinus pinaster putative auxin induced transcription facto 275 G1449gi2388689 1.50E−19 Glycine max GH1 protein. 275 G1449 gi202572192.50E−19 Zinnia elegans auxin-regulated protein. 277 G1451 AB071298 1.0e−999 Oryza sativa OsARF8 mRNA for auxin response factor 8, parti277 G1451 AY105215 1.00E−157 Zea mays PCO121637 mRNA sequence. 277 G1451AW690130 1.00E−109 Medicago truncatula NF028B12ST1F1000 Developing stemMedica 277 G1451 BQ862285 1.00E−108 Lactuca sativa QGC20K23.yg.ab1QG_ABCDI lettuce salinas Lac 277 G1451 BG597435 1.00E−107 Solanumtuberosum EST496113 cSTS Solanum tuberosum cDNA clo 277 G1451 BJ3036021.00E−104 Triticum aestivum BJ303602 Y. Ogihara unpublished cDNA libr277 G1451 OSA306306 1.00E−103 Oryza sativa (japonica Oryza sativa subsp.cultivar-group) 277 G1451 BQ595269 1.00E−89 Beta vulgarisE012710-024-023-D13-SP6 MPIZ-ADIS-024-develop 277 G1451 CA8012181.00E−86 Glycine max sau02f06.y2 Gm-c1062 Glycine max cDNA clone SOY 277G1451 BG159611 8.00E−79 Sorghum bicolor OV2_6_G07.b1_A002 Ovary 2 (OV2)Sorghum bic 277 G1451 gi19352049 3.70E−247 Oryza sativa auxin responsefactor 8. 277 G1451 gi20805236 3.10E−126 Oryza sativa (japonica auxinresponse fac cultivar-group) 277 G1451 gi24785191 4.10E−55 Nicotianatabacum hypothetical protein. 277 G1451 gi23343944 2.40E−28 Mirabilisjalapa auxin-responsive factor protein. 277 G1451 gi20269053 7.00E−10Populus tremula x aux/IAA protein. Populus tremuloides 277 G1451gi287566 3.10E−06 Vigna radiata ORF. 277 G1451 gi114733 1.10E−05 Glycinemax AUXIN-INDUCED PROTEIN AUX22. 277 G1451 gi871511 2.40E−05 Pisumsativum auxin-induced protein. 277 G1451 gi18697008 0.00027 Zea maysunnamed protein product. 277 G1451 gi17976835 0.00068 Pinus pinasterputative auxin induced transcription facto 279 G1452 BF645605 4.00E−65Medicago truncatula NF017A10EC1F1072 Elicited cell culture 279 G1452BI140703 5.00E−43 Sorghum bicolor IP1_52_F12.b1_A002 Immature pannicle 1(IP1 279 G1452 BQ469035 9.00E−43 Hordeum vulgare HM03C20r HM Hordeumvulgare cDNA clone HM03 279 G1452 BU967516 9.00E−43 Hordeum vulgaresubsp. HB04I23r BC Hordeum vulgare vulgare 279 G1452 BJ481205 9.00E−43Hordeum vulgare subsp. BJ481205 K. Sato unpublis spontaneum 279 G1452BQ620568 2.00E−42 Triticum aestivum TaLr1142G07R TaLr1 Triticum aestivumcDNA 279 G1452 AB028187 8.00E−42 Oryza sativa mRNA for OsNAC8 protein,complete cds. 279 G1452 BQ997138 3.00E−41 Lactuca sativa QGG14N12.yg.ab1QG_EFGHJ lettuce serriola La 279 G1452 BG543974 4.00E−40 Brassica rapasubsp. E1725 Chinese cabbage pekinensis etiol 279 G1452 AF5098744.00E−40 Petunia x hybrida nam-like protein 11 (NH11) mRNA, complete 279G1452 gi6730946 1.10E−44 Oryza sativa OsNAC8 protein. 279 G1452gi21105746 9.50E−42 Petunia x hybrida nam-like protein 9. 279 G1452gi7716952 4.70E−41 Medicago truncatula NAC1. 279 G1452 gi192250186.00E−41 Oryza sativa (japonica putative NAM (no a cultivar-group) 279G1452 gi22597158 4.30E−38 Glycine max no apical meristem-like protein.279 G1452 gi15148914 5.70E−36 Phaseolus vulgaris NAC domain proteinNAC2. 279 G1452 gi4218537 3.20E−35 Triticum sp. GRAB2 protein. 279 G1452gi6732160 3.20E−35 Triticum monococcum unnamed protein product. 279G1452 gi6175246 5.90E−34 Lycopersicon jasmonic acid 2. esculentum 279G1452 gi14485513 2.00E−33 Solanum tuberosum putative NAC domain protein.281 G1463 BH478066 2.00E−72 Brassica oleracea BOHQV38TR BOHQ Brassicaoleracea genomic 281 G1463 BE461560 1.00E−05 Lycopersicon EST412979tomato breaker esculentum fruit, TIG 281 G1463 AAAA01002994 1.00E−05Oryza sativa (indica ( ) scaffold002994 cultivar-group) 281 G1463AP005621 1.00E−05 Oryza sativa (japonica ( ) chromosome 6 clocultivar-group) 281 G1463 OSJN01006 1.00E−05 Oryza sativa chromosome Xclone OSJNBa0082A03, *** SEQUENC 281 G1463 BQ852361 7.00E−05 Lactucasativa QGB17N02.yg.ab1 QG_ABCDI lettuce salinas Lac 281 G1463 BG4409243.00E−04 Gossypium arboreum GA_Ea0010P20f Gossypium arboreum 7-10 d 281G1463 BU763436 4.00E−04 Glycine max sas42e12.y1 Gm-c1080 Glycine maxcDNA clone SOY 281 G1463 BM406262 5.00E−04 Solanum tuberosum EST580589potato roots Solanum tuberosum 281 G1463 AI729055 0.002 Gossypiumhirsutum BNLGHi12472 Six-day Cotton fiber Gossypi 281 G1463 gi131294971.40E−07 Oryza sativa putative NAM (no apical meristem) protein. 281G1463 gi21389176 1.30E−06 Petunia x hybrida nam-like protein 19. 281G1463 gi22002150 7.70E−05 Oryza sativa (japonica putative NAM (no acultivar-group) 281 G1463 gi6175246 0.00012 Lycopersicon jasmonic acid2. esculentum 281 G1463 gi22597158 0.00056 Glycine max no apicalmeristem-like protein. 281 G1463 gi6732156 0.013 Triticum monococcumunnamed protein product. 281 G1463 gi15148912 0.02 Phaseolus vulgarisNAC domain protein NAC1. 281 G1463 gi14485513 0.055 Solanum tuberosumputative NAC domain protein. 281 G1463 gi2982275 0.063 Picea marianaATAF1-like protein. 281 G1463 gi4218537 0.09 Triticum sp. GRAB2 protein.283 G1471 BH512970 9.00E−14 Brassica oleracea BOHIV20TF BOHI Brassicaoleracea genomic 283 G1471 BZ374146 0.015 Zea mays ie21f07.g2 WGS-ZmaysF(DH5a methyl filtered) Zea m 283 G1471 AI898615 0.02 LycopersiconEST268058 tomato ovary, esculentum TAMU Lycope 283 G1471 BG646742 0.02Medicago truncatula EST508361 HOGA Medicago truncatula cDNA 283 G1471BI968516 0.026 Glycine max GM830005B12C03 Gm- r1083 Glycine max cDNAclone 283 G1471 AP004754 0.026 Oryza sativa (japonica ( ) chromosome 6clo cultivar-group) 283 G1471 AAAA01022633 0.026 Oryza sativa (indica () scaffold022633 cultivar-group) 283 G1471 AB006606 0.034 Petunia xhybrida mRNA for ZPT4-4, complete cds. 283 G1471 BU879483 0.058 Populusbalsamifera V060G08 Populus flow subsp. trichocarpa 283 G1471 BM3597770.058 Gossypium arboreum GA_Ea0023K21r Gossypium arboreum 7-10 d 283G1471 gi439491 0.00043 Petunia x hybrida zinc-finger DNA bindingprotein. 283 G1471 gi1763063 0.0094 Glycine max SCOF-1. 283 G1471gi15623820 0.012 Oryza sativa hypothetical protein. 283 G1471 gi183901090.049 Sorghum bicolor putative zinc finger protein. 283 G1471 gi20585040.074 Brassica rapa zinc-finger protein-1. 283 G1471 gi7228329 0.095Medicago sativa putative TFIIIA (or kruppel)-like zinc fi 283 G1471gi4666360 0.13 Datisca glomerata zinc-finger protein 1. 283 G1471gi18674684 0.14 Zea ramosa unnamed protein product. 283 G1471 gi208048830.24 Oryza sativa (japonica putative zinc fing cultivar-group) 283 G1471gi2981169 0.39 Nicotiana tabacum osmotic stress-induced zinc- fingerprot 285 G1478 BH541785 8.00E−38 Brassica oleracea BOHPJ56TF BOHPBrassica oleracea genomic 285 G1478 BI122215 4.00E−23 Populus tremula xI003P84P Populus leaf Populus tremuloides 285 G1478 BF275913 5.00E−22Gossypium arboreum GA_Eb0025C07f Gossypium arboreum 7-10 d 285 G1478CA814858 1.00E−21 Vitis vinifera CA12EI201IIbF_F05 Cabernet SauvignonLeaf- 285 G1478 BG157399 8.00E−20 Glycine max sab36g12.y1 Gm-c1026Glycine max cDNA clone GEN 285 G1478 CA798224 3.00E−15 Theobroma cacaoCac_BL_5512 Cac_BL (Bean and Leaf from Amel 285 G1478 BU873581 6.00E−12Populus balsamifera Q057B04 Populus flow subsp. trichocarpa 285 G1478BU046688 2.00E−11 Prunus persica PP_LEa0027D08f Peach developing fruitmesoca 285 G1478 C95300 8.00E−11 Citrus unshiu C95300 Citrus unshiuMiyagawa-wase maturation 285 G1478 BQ594583 1.00E−10 Beta vulgarisE012444-024-024-P06-SP6 MPIZ-ADIS-024-develop 285 G1478 gi28951881.10E−11 Brassica napus CONSTANS homolog. 285 G1478 gi3618308 3.50E−10Oryza sativa zinc finger protein. 285 G1478 gi23495871 1.10E−09 Oryzasativa (japonica putative zinc-fing cultivar-group) 285 G1478 gi110373081.10E−09 Brassica nigra constans-like protein. 285 G1478 gi33417233.10E−09 Raphanus sativus CONSTANS-like 1 protein. 285 G1478 gi40918063.60E−08 Malus x domestica CONSTANS-like protein 2. 285 G1478 gi216551683.70E−08 Hordeum vulgare subsp. CONSTANS-like protein vulgare CO8. 285G1478 gi21667475 4.50E−08 Hordeum vulgare CONSTANS-like protein. 285G1478 gi10946337 7.20E−08 Ipomoea nil CONSTANS-like protein. 285 G1478gi4557093 3.30E−06 Pinus radiata zinc finger protein. 287 G1482 BM4062019.00E−61 Solanum tuberosum EST580528 potato roots Solanum tuberosum 287G1482 BF644868 1.00E−53 Medicago truncatula NF023D11EC1F1093 Elicitedcell culture 287 G1482 BI678186 9.00E−53 Robinia pseudoacacia CLS1114CLS (Cambium and bark region o 287 G1482 BM954087 4.00E−52 Glycine maxsam70a09.y1 Gm-c1069 Glycine max cDNA clone SOY 287 G1482 BI4202511.00E−48 Lotus japonicus LjNEST54g9r Lotus japonicus nodule library 287G1482 AU288043 1.00E−45 Zinnia elegans AU288043 zinnia culturedmesophyll cell equa 287 G1482 BU892726 2.00E−45 Populus tremula P068F06Populus petioles cDNA library Popul 287 G1482 BE432467 1.00E−44Lycopersicon EST398996 tomato breaker esculentum fruit, TIG 287 G1482AB001884 4.00E−43 Oryza sativa mRNA for zinc finger protein, completecds, 287 G1482 BZ088073 6.00E−43 Brassica oleracea lla97a06.b1 B.oleracea002 Brassica olerac 287 G1482 gi3618312 1.60E−45 Oryza sativazinc finger protein. 287 G1482 gi11037311 4.00E−18 Brassica nigraconstans-like protein. 287 G1482 gi3341723 6.50E−17 Raphanus sativusCONSTANS-like 1 protein. 287 G1482 gi23589949 5.50E−16 Oryza sativa(japonica Hd1. cultivar-group) 287 G1482 gi4091806 6.00E−15 Malus xdomestica CONSTANS-like protein 2. 287 G1482 gi10946337 1.60E−14 Ipomoeanil CONSTANS-like protein. 287 G1482 gi2303681 2.10E−14 Brassica napusunnamed protein product. 287 G1482 gi21667485 2.30E−13 Hordeum vulgareCONSTANS-like protein. 287 G1482 gi21655154 1.20E−11 Hordeum vulgaresubsp. CONSTANS-like protein vulgare CO5. 287 G1482 gi4557093 2.50E−10Pinus radiata zinc finger protein. 289 G1488 BH447680 5.00E−83 Brassicaoleracea BOHQJ20TR BOHQ Brassica oleracea genomic 289 G1488 AP0033767.00E−55 Oryza sativa chromosome 1 clone OSJNBa0014K08, *** SEQUENCI 289G1488 AAAA01003594 3.00E−54 Oryza sativa (indica ( ) scaffold003594cultivar-group) 289 G1488 AC132491 3.00E−54 Oryza sativa (japonica ( )chromosome 5 clo cultivar-group) 289 G1488 BQ851743 3.00E−49 Lactucasativa QGB16C22.yg.ab1 QG_ABCDI lettuce salinas Lac 289 G1488 BM1132283.00E−49 Solanum tuberosum EST560764 potato roots Solanum tuberosum 289G1488 BU547281 2.00E−45 Glycine max GM880012B20D06 Gm- r1088 Glycine maxcDNA clone 289 G1488 BQ410000 3.00E−43 Gossypium arboreum GA_Ed0026H09rGossypium arboreum 7-10 d 289 G1488 CA600585 6.00E−38 Triticum aestivumwaw1c.pk005.k20 waw1c Triticum aestivum c 289 G1488 AC136451 2.00E−36Medicago truncatula clone mth2-17d19, WORKING DRAFT SEQUENC 289 G1488gi21902044 1.80E−48 Oryza sativa (japonica hypothetical protecultivar-group) 289 G1488 gi14165317 4.10E−42 Oryza sativa putativetranscription factor. 289 G1488 gi12711287 3.80E−30 Nicotiana tabacumGATA-1 zinc finger protein. 289 G1488 gi1076609 5.60E−22 Nicotiana NTL1protein-curled- plumbaginifolia leaved to 289 G1488 gi14550106 0.85 Zeamays HD2 type histone deacetylase HDA106. 289 G1488 gi21953514 0.98 Zeamays subsp. ZAGL1. parviglumis 289 G1488 gi21953536 1 Zea mays subsp.mays ZAGL1. 291 G1494 BH695524 1.00E−66 Brassica oleracea BOMMP13TFBO_2_3_KB Brassica oleracea gen 291 G1494 BU866069 2.00E−47 Populustremula x S062C11 Populus imbib Populus tremuloides 291 G1494 BG5910631.00E−37 Solanum tuberosum EST498905 P. infestans- challenged leaf So291 G1494 BF518953 2.00E−36 Medicago truncatula EST456346 DSIL Medicagotruncatula cDNA 291 G1494 BM411362 1.00E−35 Lycopersicon EST585689tomato breaker esculentum fruit Lyco 291 G1494 BE598711 5.00E−30 Sorghumbicolor PI1_81_D03.b1_A002 Pathogen induced 1 (PI1) 291 G1494 BU5743186.00E−30 Prunus dulcis PA_Ea0007A10f Almond developing seed Prunus 291G1494 CA008614 1.00E−29 Hordeum vulgare subsp. HU11I14r HU Hordeumvulgare vulgare 291 G1494 BG041496 3.00E−29 Glycine max sv35a08.y1Gm-c1057 Glycine max cDNA clone GENO 291 G1494 BG052163 3.00E−27 Sorghumpropinquum RHIZ2_6_H10.b1_A003 Rhizome2 (RHIZ2) Sor 291 G1494 gi234957421.90E−39 Oryza sativa (japonica putative phytochro cultivar-group) 291G1494 gi13486760 4.50E−25 Oryza sativa hypothetical protein. 291 G1494gi5923912 2.00E−10 Tulipa gesneriana bHLH transcription factor GBOF-1.291 G1494 gi1086538 2.30E−09 Oryza rufipogon transcriptional activatorRb homolog. 291 G1494 gi527657 1.00E−08 Pennisetum glaucum myc-likeregulatory R gene product. 291 G1494 gi527665 3.60E−08 Sorghum bicolormyc-like regulatory R gene product. 291 G1494 gi527661 7.60E−08Phyllostachys acuta myc-like regulatory R gene product. 291 G1494gi1086534 4.40E−07 Oryza officinalis transcriptional activator Rahomolog. 291 G1494 gi527663 4.40E−07 Tripsacum australe myc-likeregulatory R gene product. 291 G1494 gi1142621 4.80E−07 Phaseolusvulgaris phaseolin G-box binding protein PG2. 293 G1496 BZ0077862.00E−64 Brassica oleracea oed22d06.g1 B. oleracea002 Brassica olerac293 G1496 BQ875608 3.00E−41 Lactuca sativa QGI8J14.yg.ab1 QG_ABCDIlettuce salinas Lact 293 G1496 BU081702 2.00E−40 Glycine max saq98c07.y1Gm-c1049 Glycine max cDNA clone SOY 293 G1496 CA525194 3.00E−37 Capsicumannuum KS12050G08 KS12 Capsicum annuum cDNA, mRNA 293 G1496 BU7911311.00E−36 Populus balsamifera subsp. trichocarpa x Populus deltoides 293G1496 AW906522 2.00E−34 Solanum tuberosum EST342644 potato stolon,Cornell Universi 293 G1496 BF273293 2.00E−34 Gossypium arboreumGA_Eb0017H08f Gossypium arboreum 7-10 d 293 G1496 BJ267378 6.00E−34Triticum aestivum BJ267378 Y. Ogihara unpublished cDNA libr 293 G1496BM497415 4.00E−33 Avicennia marina 901269 Avicennia marina leaf cDNALibrary 293 G1496 CA003238 1.00E−32 Hordeum vulgare subsp. HS09N06r HSHordeum vulgare vulgare 293 G1496 gi20804997 5.10E−35 Oryza sativa(japonica DNA-binding protei cultivar-group) 293 G1496 gi118629649.50E−35 Oryza sativa hypothetical protein. 293 G1496 gi5923912 7.00E−31Tulipa gesneriana bHLH transcription factor GBOF-1. 293 G1496 gi61662831.70E−10 Pinus taeda helix-loop-helix protein 1A. 293 G1496 gi5276552.00E−05 Pennisetum glaucum myc-like regulatory R gene product. 293G1496 gi527665 2.90E−05 Sorghum bicolor myc-like regulatory R geneproduct. 293 G1496 gi527661 5.50E−05 Phyllostachys acuta myc-likeregulatory R gene product. 293 G1496 gi1086538 0.00019 Oryza rufipogontranscriptional activator Rb homolog. 293 G1496 gi4206118 0.00024Mesembryanthemum transporter homolog. crystallinum 293 G1496 gi33997770.00025 Glycine max symbiotic ammonium transporter; nodulin. 295 G1499AT002234 1.00E−53 Brassica rapa subsp. AT002234 Flower bud pekinensiscDNA Br 295 G1499 AP004462 1.00E−46 Oryza sativa (japonica ( )chromosome 8 clo cultivar-group) 295 G1499 AAAA01003354 1.00E−46 Oryzasativa (indica ( ) scaffold003354 cultivar-group) 295 G1499 BH7758061.00E−39 Zea mays fzmb011f018c05f1 fzmb filtered library Zea mays ge 295G1499 BH700922 4.00E−35 Brassica oleracea BOMMZ07TR BO_2_3_KB Brassicaoleracea gen 295 G1499 AP004693 1.00E−34 Oryza sativa chromosome 8 cloneP0461F06, *** SEQUENCING IN 295 G1499 AW832545 5.00E−34 Glycine maxsm12e10.y1 Gm-c1027 Glycine max cDNA clone GENO 295 G1499 BE4511741.00E−32 Lycopersicon EST402062 tomato root, esculentum plants pre-a 295G1499 BF263465 4.00E−25 Hordeum vulgare HV_CEa0006N02f Hordeum vulgareseedling gre 295 G1499 BG557011 5.00E−22 Sorghum bicolorEM1_41_E02.g1_A002 Embryo 1 (EM1) Sorghum b 295 G1499 gi155287432.50E−30 Oryza sativa contains EST C74560(E31855)~unknown protein. 295G1499 gi19571105 2.80E−27 Oryza sativa (japonica hypothetical protecultivar-group) 295 G1499 gi11045087 1.10E−08 Brassica napus putativeprotein. 295 G1499 gi3127045 6.20E−08 Petunia x hybrida bHLHtranscription factor JAF13. 295 G1499 gi1086538 1.30E−07 Oryza rufipogontranscriptional activator Rb homolog. 295 G1499 gi6166283 1.60E−07 Pinustaeda helix-loop-helix protein 1A. 295 G1499 gi5923912 1.00E−06 Tulipagesneriana bHLH transcription factor GBOF-1. 295 G1499 gi56696561.10E−06 Lycopersicon ER33 protein. esculentum 295 G1499 gi5276651.40E−06 Sorghum bicolor myc-like regulatory R gene product. 295 G1499gi1086534 3.10E−06 Oryza officinalis transcriptional activator Rahomolog. 297 G1519 AY107434 1.00E−131 Zea mays PCO110680 mRNA sequence.297 G1519 BQ579759 4.00E−68 Triticum aestivum WHE2974_B12_D24ZS Wheatdormant embryo cD 297 G1519 BQ851827 3.00E−66 Lactuca sativaQGB16G12.yg.ab1 QG_ABCDI lettuce salinas Lac 297 G1519 BM094986 2.00E−61Glycine max saj24f10.y1 Gm-c1066 Glycine max cDNA clone GEN 297 G1519BE354396 3.00E−54 Lycopersicon EST355739 tomato flower esculentum buds,anthe 297 G1519 AW618704 7.00E−52 Lycopersicon pennellii EST320690 L.pennellii trichome, Cor 297 G1519 BF004323 4.00E−50 Medicago truncatulaEST432821 KV1 Medicago truncatula cDNA 297 G1519 BU833376 2.00E−49Populus tremula x T047C03 Populus apica Populus tremuloides 297 G1519AJ470209 3.00E−45 Hordeum vulgare AJ470209 S00008 Hordeum vulgare cDNAclone 297 G1519 BF053939 2.00E−43 Solanum tuberosum EST439169 potatoleaves and petioles Sola 297 G1519 gi14192879 1.00E−88 Oryza sativaPutative zinc-binding peroxisomal integral m 297 G1519 gi225355778.90E−08 Oryza sativa (japonica hypothetical prote cultivar-group) 297G1519 gi22795037 4.30E−07 Populus x canescens putative RING protein. 297G1519 gi9294812 0.0026 Medicago truncatula MTD2. 297 G1519 gi181292860.0028 Pinus pinaster putative RING zinc finger protein. 297 G1519gi4090943 0.0029 Lycopersicon COP1 homolog. esculentum 297 G1519gi22775495 0.0095 Arabis gemmifera similar to A. thaliana AT4g08590. 297G1519 gi20340241 0.021 Thellungiella halophila putative RING zinc fingerprotein 297 G1519 gi1694900 0.022 Pisum sativum Cop1 protein. 297 G1519gi7592844 0.029 Oryza sativa subsp. COP1. japonica 299 G1526AAAA01000691 1.00E−103 Oryza sativa (indica ( ) scaffold000691cultivar-group) 299 G1526 BG599126 1.00E−101 Solanum tuberosum EST504026cSTS Solanum tuberosum cDNA clo 299 G1526 BI098460 7.00E−94 Sorghumbicolor IP1_32_F12.b1_A002 Immature pannicle 1 (IP1 299 G1526 AY1105824.00E−74 Zea mays CL19105_1 mRNA sequence. 299 G1526 AJ468417 8.00E−69Hordeum vulgare AJ468417 S00008 Hordeum vulgare cDNA clone 299 G1526AL819754 9.00E−68 Triticum aestivum AL819754 n: 129 Triticum aestivumcDNA clo 299 G1526 AW011575 2.00E−66 Pinus taeda ST22D10 Pine TriplExshoot tip library Pinus ta 299 G1526 AW704900 6.00E−63 Glycine maxsk40h12.y1 Gm-c1019 Glycine max cDNA clone GENO 299 G1526 AP0048794.00E−60 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 299G1526 BQ589890 1.00E−57 Beta vulgaris S015141-024-019-P15-SP6MPIZ-ADIS-024-storage 299 G1526 gi23237908 4.20E−115 Oryza sativa(japonica helicase-like tran cultivar-group) 299 G1526 gi152898725.60E−80 Oryza sativa putative helicase-like transcription factor. 299G1526 gi18463957 1.90E−42 Zea mays chromatin complex subunit A101. 299G1526 gi23193481 8.70E−32 Hordeum vulgare SNF2P. 299 G1526 gi231934879.70E−32 Triticum monococcum SNF2P. 299 G1526 gi23193479 2.50E−30Hordeum vulgare subsp. SNF2P. vulgare 299 G1526 gi15029364 0.0012 Rosahybrid cultivar photoregulatory zinc-finger protein 299 G1526 gi16949000.0052 Pisum sativum Cop1 protein. 299 G1526 gi7592844 0.011 Oryzasativa subsp. COP1. japonica 299 G1526 gi4090943 0.014 Lycopersicon COP1homolog. esculentum 301 G1540 BZ081838 4.00E−70 Brassica oleraceallf51h03.g1 B. oleracea002 Brassica olerac 301 G1540 AF481951 7.00E−41Petunia x hybrida wuschel protein (WUS) mRNA, complete cds. 301 G1540AAAA01000169 2.00E−28 Oryza sativa (indica ( ) scaffold000169cultivar-group) 301 G1540 OSJN00127 2.00E−28 Oryza sativa chromosome 4clone OSJNBA0084K01, *** SEQUENC 301 G1540 AX105289 2.00E−26 Zea maysSequence 7 from Patent WO0123575. 301 G1540 AC137078 2.00E−21 Medicagotruncatula clone mth2-10e12, WORKING DRAFT SEQUENC 301 G1540 BI2043695.00E−20 Lycopersicon EST522409 cTOS esculentum Lycopersicon esculen 301G1540 CNS08CDT 1.00E−19 Oryza sativa (japonica ( ) chromosome 12 clcultivar-group) 301 G1540 BU006325 2.00E−19 Lactuca sativaQGH10L09.yg.ab1 QG_EFGHJ lettuce serriola La 301 G1540 AF322401 3.00E−19Vigna radiata clone LR129 microsatellite sequence. 301 G1540 gi220871285.10E−35 Petunia x hybrida wuschel protein. 301 G1540 gi8099120 3.90E−21Oryza sativa similar to a putative homeodomain transcript 301 G1540gi21104626 6.40E−21 Oryza sativa (japonica hypothetical protecultivar-group) 301 G1540 gi3955021 1.80E−09 Populus tremula x HB2homeodomain pro Populus tremuloides 301 G1540 gi18419580 0.00033Narcissus putative homeobox- pseudonarcissus containing pr 301 G1540gi3868829 0.0014 Ceratopteris richardii CRHB1. 301 G1540 gi244171470.0024 Zinnia elegans homeobox leucine-zipper protein. 301 G1540gi7209912 0.14 Physcomitrella patens homeobox protein PpHB10. 301 G1540gi13365610 0.74 Pisum sativum SCARECROW. 301 G1540 gi1160484 0.94Pimpinella brachycarpa homeobox-leucine zipper protein. 303 G1543AF145727 4.00E−51 Oryza sativa homeodomain leucine zipper protein (hox3)mRNA 303 G1543 CA030381 6.00E−41 Hordeum vulgare subsp. HX06O07r HXHordeum vulgare vulgare 303 G1543 BQ741095 6.00E−39 Glycine maxsaq14c10.y1 Gm-c1045 Glycine max cDNA clone SOY 303 G1543 AT0021181.00E−38 Brassica rapa subsp. AT002118 Flower bud pekinensis cDNA Br 303G1543 BQ857226 2.00E−37 Lactuca sativa QGB6P03.yg.ab1 QG_ABCDI lettucesalinas Lact 303 G1543 AB028075 4.00E−37 Physcomitrella patens mRNA forhomeobox protein PpHB4, comp 303 G1543 PBPHZ4GEN 4.00E−37 Pimpinellabrachycarpa P. brachycarpa mRNA for homeobox-leu 303 G1543 LEHDZIPP5.00E−37 Lycopersicon L. esculentum mRNA for esculentum HD-ZIP protei303 G1543 AF443619 1.00E−36 Craterostigma homeodomain leucineplantagineum zipper prote 303 G1543 AJ498394 2.00E−36 Medicagotruncatula AJ498394 MTPOSE Medicago truncatula cDN 303 G1543 gi50068518.30E−51 Oryza sativa homeodomain leucine zipper protein. 303 G1543gi20161555 1.70E−50 Oryza sativa (japonica putative homeodomacultivar-group) 303 G1543 gi18034437 1.60E−38 Craterostigma homeodomainleucine plantagineum zipper pro 303 G1543 gi1149535 4.30E−38 Pimpinellabrachycarpa homeobox-leucine zipper protein. 303 G1543 gi992598 1.20E−37Lycopersicon HD-ZIP protein. esculentum 303 G1543 gi7415620 1.50E−37Physcomitrella patens homeobox protein PpHB4. 303 G1543 gi12349003.10E−37 Glycine max homeobox-leucine zipper protein. 303 G1543gi3868847 1.90E−35 Ceratopteris richardii CRHB10. 303 G1543 gi89198761.90E−35 Capsella rubella hypothetical protein. 303 G1543 gi10323723.20E−35 Helianthus annuus homeodomain protein. 305 G1634 AW1642756.00E−63 Glycine max se70d01.y1 Gm-c1023 Glycine max cDNA clone GENO 305G1634 AF239956 3.00E−60 Hevea brasiliensis unknown mRNA. 305 G1634BQ115848 9.00E−58 Solanum tuberosum EST601424 mixed potato tissuesSolanum tu 305 G1634 AW220831 5.00E−53 Lycopersicon EST297300 tomatofruit esculentum mature green 305 G1634 BQ992139 9.00E−53 Lactuca sativaQGF24M24.yg.ab1 QG_EFGHJ lettuce serriola La 305 G1634 BG525326 3.00E−46Stevia rebaudiana 48-70 Stevia field grown leaf cDNA Stevia 305 G1634BE319813 2.00E−45 Medicago truncatula NF022C09RT1F1066 Developing rootMedica 305 G1634 AP003279 3.00E−45 Oryza sativa chromosome 1 cloneP0529E05, *** SEQUENCING IN 305 G1634 AAAA01017329 3.00E−45 Oryza sativa(indica ( ) scaffold017329 cultivar-group) 305 G1634 AC130612 3.00E−45Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 305 G1634gi12005328 7.40E−59 Hevea brasiliensis unknown. 305 G1634 gi188742631.10E−55 Antirrhinum majus MYB-like transcription factor DIVARICAT 305G1634 gi18461206 2.80E−50 Oryza sativa (japonica contains ESTs AU10cultivar-group) 305 G1634 gi10798825 2.10E−45 Oryza sativa putativemyb-related transcription activator 305 G1634 gi19911579 6.60E−42Glycine max syringolide-induced protein 1-3-1B. 305 G1634 gi152091762.00E−41 Solanum demissum putative I-box binding factor. 305 G1634gi6688529 2.30E−39 Lycopersicon I-box binding factor. esculentum 305G1634 gi12406995 3.30E−24 Hordeum vulgare MCB2 protein. 305 G1634gi7705206 2.30E−23 Solanum tuberosum MybSt1. 305 G1634 gi200676613.40E−18 Zea mays one repeat myb transcriptional factor. 307 G1637BZ011351 4.00E−81 Brassica oleracea oed23f03.b1 B. oleracea002 Brassicaolerac 307 G1637 BE033910 3.00E−42 Mesembryanthemum MG01H12 MGcrystallinum Mesembryanthemum c 307 G1637 AY151044 9.00E−39 Oryza sativa(japonica ( ) transcription fa cultivar-group) 307 G1637 BU8327072.00E−38 Populus tremula x T037C12 Populus apica Populus tremuloides 307G1637 CA728673 3.00E−38 Triticum aestivum wdi1c.pk004.124 wdi1c Triticumaestivum c 307 G1637 BG454685 1.00E−37 Medicago truncatulaNF102F10LF1F1080 Developing leaf Medica 307 G1637 CA799375 1.00E−37Glycine max sat32h04.y1 Gm-c1056 Glycine max cDNA clone SOY 307 G1637BJ472691 2.00E−37 Hordeum vulgare subsp. BJ472691 K. Sato vulgareunpublished 307 G1637 CA813590 3.00E−37 Vitis vinifera CA48LU10IVF-G11CA48LU Vitis vinifera cDNA c 307 G1637 BQ114109 8.00E−37 Solanumtuberosum EST599685 mixed potato tissues Solanum tu 307 G1637 gi135699961.10E−39 Oryza sativa putative Myb-related protein. 307 G1637 gi248503072.30E−39 Oryza sativa (japonica transcription fact cultivar-group) 307G1637 gi1076660 1.70E−36 Solanum tuberosum D13F(MYBST1) protein- potato.307 G1637 gi12406993 8.90E−31 Hordeum vulgare MCB1 protein. 307 G1637gi12005328 3.60E−27 Hevea brasiliensis unknown. 307 G1637 gi188742632.50E−26 Antirrhinum majus MYB-like transcription factor DIVARICAT 307G1637 gi19911577 1.40E−25 Glycine max syringolide-induced protein1-3-1A. 307 G1637 gi6688529 3.00E−23 Lycopersicon I-box binding factor.esculentum 307 G1637 gi15209176 7.60E−21 Solanum demissum putative I-boxbinding factor. 307 G1637 gi20067661 3.40E−16 Zea mays one repeat mybtranscriptional factor. 309 G1640 AF034132 3.00E−60 Gossypium hirsutumMYB-like DNA-binding domain protein (Cmy 309 G1640 AV421866 6.00E−57Lotus japonicus AV421866 Lotus japonicus young plants (two- 309 G1640ZMU57002 8.00E−57 Zea mays P protein (P) mRNA, complete cds. 309 G1640BI924574 2.00E−56 Lycopersicon EST544463 tomato flower, esculentum buds0-3 m 309 G1640 AW255172 2.00E−55 Mentha x piperita ML160 peppermintglandular trichome Menth 309 G1640 BE558747 3.00E−54 Hordeum vulgareHV_CEb0020E02f Hordeum vulgare seedling gre 309 G1640 AW186273 1.00E−51Glycine max se65f12.y1 Gm-c1019 Glycine max cDNA clone GENO 309 G1640PMU39448 2.00E−50 Picea mariana MYB-like transcriptional factor MBF1mRNA, co 309 G1640 BQ865372 3.00E−50 Lactuca sativa QGC4a02.yg.ab1QG_ABCDI lettuce salinas Lact 309 G1640 BQ046535 8.00E−49 Solanumtuberosum EST595653 P. infestans- challenged potato 309 G1640 gi120605327.40E−59 Oryza sativa putative myb-related protein P. 309 G1640gi2921336 2.00E−58 Gossypium hirsutum MYB-like DNA-binding domainprotein. 309 G1640 gi11526779 2.00E−56 Zea mays subsp. P-like protein.parviglumis 309 G1640 gi11526773 5.40E−56 Zea mays P2 protein. 309 G1640gi1101770 4.60E−50 Picea mariana MYB-like transcriptional factor MBF1.309 G1640 gi82308 8.60E−49 Antirrhinum majus myb protein 308-gardensnapdragon. 309 G1640 gi1370140 8.70E−49 Lycopersicon myb-relatedtranscription esculentum factor. 309 G1640 gi5139802 1.80E−48 Glycinemax GmMYB29A1. 309 G1640 gi127579 1.30E−47 Hordeum vulgare MYB-RELATEDPROTEIN HV1. 309 G1640 gi227030 1.30E−47 Hordeum vulgare var.myb-related gene Hv1. distichum 311 G1645 AW624217 9.00E−59 LycopersiconEST322258 tomato flower esculentum buds 3-8 mm 311 G1645 AQ9170841.00E−54 Medicago truncatula T233110b Medicago truncatula BAC librar 311G1645 AP005757 4.00E−53 Oryza sativa (japonica ( ) chromosome 8 clocultivar-group) 311 G1645 AAAA01001041 4.00E−53 Oryza sativa (indica ( )scaffold001041 cultivar-group) 311 G1645 BQ514458 7.00E−53 Solanumtuberosum EST621873 Generation of a set of potato c 311 G1645 BF2705113.00E−51 Gossypium arboreum GA_Eb0008O08f Gossypium arboreum 7-10 d 311G1645 AP000837 7.00E−51 Oryza sativa genomic DNA, chromosome 1, clone:P0424A08. 311 G1645 AX288143 5.00E−49 Physcomitrella patens Sequence 14from Patent WO0177311. 311 G1645 AI164087 1.00E−48 Populus tremula xA054P76U Hybrid aspen Populus tremuloides 311 G1645 BQ623005 1.00E−46Citrus sinensis USDA-FP_00096 Ridge pineapple sweet orange 311 G1645gi6539552 4.80E−58 Oryza sativa Similar to putative transcription factor(AF 311 G1645 gi21321780 1.40E−46 Oryza sativa (japonica putativeMyb/Myb-r cultivar-group) 311 G1645 gi9954112 2.10E−34 Solanum tuberosumtuber-specific and sucrose- responsive e 311 G1645 gi20565 2.30E−29Petunia x hybrida protein 3. 311 G1645 gi7230673 6.40E−29 Papaver rhoeasputative Myb-related domain. 311 G1645 gi16326133 2.80E−28 Nicotianatabacum Myb. 311 G1645 gi8745321 2.90E−28 Physcomitrella patens putativec-myb-like transcription f 311 G1645 gi8745325 3.10E−28 Hordeum vulgareputative c-myb-like transcription factor. 311 G1645 gi7677132 5.10E−28Secale cereale c-myb-like transcription factor. 311 G1645 gi76771361.30E−27 Adiantum raddianum c-myb-like transcription factor. 313 G1646AW776719 3.00E−88 Medicago truncatula EST335784 DSIL Medicago truncatulacDNA 313 G1646 BG591677 6.00E−87 Solanum tuberosum EST499519 P.infestans- challenged leaf So 313 G1646 BQ411597 4.00E−85 Gossypiumarboreum GA_Ed0041B06f Gossypium arboreum 7-10 d 313 G1646 BE2089172.00E−84 Citrus x paradisi GF-FV-P3F5 Marsh grapefruit young flavedo 313G1646 BM065544 1.00E−83 Capsicum annuum KS07004F12 KS07 Capsicum annuumcDNA, mRNA 313 G1646 BQ860015 1.00E−79 Lactuca sativa QGC14J23.yg.ab1QG_ABCDI lettuce salinas Lac 313 G1646 BI701620 3.00E−79 Glycine maxsai18a04.y1 Gm-c1053 Glycine max cDNA clone GEN 313 G1646 BH7253542.00E−77 Brassica oleracea BOHVO37TF BO_2_3_KB Brassica oleracea gen 313G1646 AW093662 2.00E−73 Lycopersicon EST286842 tomato mixed esculentumelicitor, BT 313 G1646 BI127986 7.00E−67 Populus tremula x G069P33YPopulus camb Populus tremuloides 313 G1646 gi5257260 6.10E−48 Oryzasativa Similar to sequence of BAC F7G19 from Arabid 313 G1646 gi208044422.30E−21 Oryza sativa (japonica hypothetical prote cultivar-group) 313G1646 gi18481626 5.00E−08 Zea mays repressor protein. 313 G1646 gi1693450.028 Phaseolus vulgaris hydroxyproline-rich glycoprotein. 313 G1646gi19700533 0.039 Pyrus communis unnamed protein product. 313 G1646gi2108256 0.095 Bromheadia extensin. finlaysoniana 313 G1646 gi17780970.1 Pinus taeda expansin. 313 G1646 gi347455 0.12 Glycine maxhydroxyproline-rich glycoprotein. 313 G1646 gi4105119 0.26 Hordeumvulgare dehydrin 10. 313 G1646 gi1076601 0.39 Lycopersicon structuralcell wall protein- esculentum to 315 G1652 AI896266 6.00E−45Lycopersicon EST265709 tomato callus, esculentum TAMU Lycop 315 G1652AI967554 2.00E−44 Lotus japonicus Ljirnpest05-403-e2 Ljirnp LambdaHybriZap t 315 G1652 BU884552 2.00E−43 Populus tremula x R012C01 Populusroot Populus tremuloides 315 G1652 AF069738 1.00E−42 Glycine maxsymbiotic ammonium transporter (SAT1) mRNA, com 315 G1652 AW7757122.00E−40 Medicago truncatula EST334777 DSIL Medicago truncatula cDNA 315G1652 AF097665 3.00E−40 Mesembryanthemum transporter homolog mRNA,crystallinum par 315 G1652 AAAA01000416 4.00E−36 Oryza sativa (indica () scaffold000416 cultivar-group) 315 G1652 BQ483543 7.00E−31 Triticumaestivum WHE3509_H02_O03ZS Wheat unstressed root c 315 G1652 AC0997321.00E−26 Oryza sativa (japonica ( ) chromosome 3 clo cultivar-group) 315G1652 BF253652 2.00E−24 Hordeum vulgare HVSMEf0001L22f Hordeum vulgareseedling roo 315 G1652 gi3399777 6.40E−44 Glycine max symbiotic ammoniumtransporter; nodulin. 315 G1652 gi4206118 8.50E−42 Mesembryanthemumtransporter homolog. crystallinum 315 G1652 gi20532320 1.10E−32 Oryzasativa (japonica Putative bHLH tran cultivar-group) 315 G1652 gi185429312.40E−28 Oryza sativa Putative bHLH transcription factor. 315 G1652gi1142619 2.70E−23 Phaseolus vulgaris phaseolin G-box binding proteinPG1. 315 G1652 gi4321762 4.30E−17 Zea mays transcription factor MYC7E.315 G1652 gi6175252 1.10E−14 Lycopersicon jasmonic acid 3. esculentum315 G1652 gi10998404 1.80E−13 Petunia x hybrida anthocyanin 1. 315 G1652gi527657 1.80E−12 Pennisetum glaucum myc-like regulatory R gene product.315 G1652 gi527661 5.10E−12 Phyllostachys acuta myc-like regulatory Rgene product. 317 G1672 BQ148509 9.00E−86 Medicago truncatulaNF069A08FL1F1065 Developing flower Medi 317 G1672 BH478545 4.00E−82Brassica oleracea BOHSE63TR BOHS Brassica oleracea genomic 317 G1672BI129724 3.00E−72 Populus tremula x G094P85Y Populus camb Populustremuloides 317 G1672 BI960052 5.00E−71 Hordeum vulgare HVSMEn0023A06fHordeum vulgare rachis EST 1 317 G1672 AC124143 5.00E−69 Oryza sativa(japonica ( ) chromosome 5 clo cultivar-group) 317 G1672 AAAA010110287.00E−69 Oryza sativa (indica ( ) scaffold011028 cultivar-group) 317G1672 BM527360 3.00E−66 Glycine max sal60h11.y1 Gm-c1061 Glycine maxcDNA clone SOY 317 G1672 BF518231 2.00E−65 Pinus taeda NXSI_036_F03_FNXSI (Nsf Xylem Side wood Inclin 317 G1672 BQ508125 8.00E−61 Solanumtuberosum EST615540 Generation of a set of potato c 317 G1672 BE4035091.00E−58 Triticum aestivum WHE0427_D02_H03ZS Wheat etiolated seedlin 317G1672 gi9049470 7.10E−78 Oryza sativa hypothetical protein. 317 G1672gi18461166 6.30E−69 Oryza sativa (japonica contains ESTs AU09cultivar-group) 317 G1672 gi12751304 1.60E−47 Brassica napus CUC2-likeprotein. 317 G1672 gi7716952 5.40E−07 Medicago truncatula NAC1. 317G1672 gi6732156 9.90E−07 Triticum monococcum unnamed protein product.317 G1672 gi21389170 1.50E−06 Petunia x hybrida nam-like protein 16. 317G1672 gi6175246 3.10E−06 Lycopersicon jasmonic acid 2. esculentum 317G1672 gi4218537 0.00019 Triticum sp. GRAB2 protein. 317 G1672 gi151489120.00051 Phaseolus vulgaris NAC domain protein NAC1. 317 G1672 gi225971580.00071 Glycine max no apical meristem-like protein. 319 G1677 BU9262687.00E−68 Glycine max sas88f08.y1 Gm-c1036 Glycine max cDNA clone SOY 319G1677 BH519017 2.00E−59 Brassica oleracea BOHHW49TR BOHH Brassicaoleracea genomic 319 G1677 BF649854 4.00E−58 Medicago truncatulaNF085A08EC1F1055 Elicited cell culture 319 G1677 BI422020 3.00E−57Lycopersicon EST532686 tomato callus, esculentum TAMU Lycop 319 G1677BU894596 3.00E−48 Populus tremula x X011H04 Populus wood Populustremuloides 319 G1677 BF625246 1.00E−47 Hordeum vulgare HVSMEa0008A15fHordeum vulgare seedling sho 319 G1677 CA810372 1.00E−46 Vitis viniferaCA22LI05IF-C9 CA22LI Vitis vinifera cDNA clo 319 G1677 BQ118483 2.00E−46Solanum tuberosum EST604059 mixed potato tissues Solanum tu 319 G1677AB028183 2.00E−46 Oryza sativa mRNA for OsNAC4 protein, complete cds.319 G1677 AF402603 6.00E−46 Phaseolus vulgaris NAC domain protein NAC2mRNA, complete c 319 G1677 gi20303588 3.40E−54 Oryza sativa (japonicaputative NAM (no a cultivar-group) 319 G1677 gi10697197 8.70E−49 Oryzasativa putative NAM protein. 319 G1677 gi21105748 5.30E−47 Petunia xhybrida nam-like protein 10. 319 G1677 gi4218535 1.80E−44 Triticum sp.GRAB1 protein. 319 G1677 gi6732158 1.80E−44 Triticum monococcum unnamedprotein product. 319 G1677 gi14485513 1.60E−43 Solanum tuberosumputative NAC domain protein. 319 G1677 gi15148914 4.10E−42 Phaseolusvulgaris NAC domain protein NAC2. 319 G1677 gi6175246 9.70E−41Lycopersicon jasmonic acid 2. esculentum 319 G1677 gi22597158 1.00E−38Glycine max no apical meristem-like protein. 319 G1677 gi77169527.30E−38 Medicago truncatula NAC1. 321 G1749 BH723520 3.00E−44 Brassicaoleracea BOHTN77TF BO_2_3_KB Brassica oleracea gen 321 G1749 AW5593743.00E−22 Medicago truncatula EST314422 DSIR Medicago truncatula cDNA 321G1749 AW152963 1.00E−17 Glycine max se33c03.y1 Gm-c1015 Glycine max cDNAclone GENO 321 G1749 BI422101 2.00E−16 Lycopersicon EST532767 tomatocallus, esculentum TAMU Lycop 321 G1749 AP005418 2.00E−15 Oryza sativa(japonica ( ) chromosome 9 clo cultivar-group) 321 G1749 AAAA010029322.00E−15 Oryza sativa (indica ( ) scaffold002932 cultivar-group) 321G1749 BU998389 2.00E−14 Hordeum vulgare subsp. HI10O11r HI Hordeumvulgare vulgare 321 G1749 BQ469024 4.00E−14 Hordeum vulgare HM03C08r HMHordeum vulgare cDNA clone HM03 321 G1749 CA728820 4.00E−13 Triticumaestivum wdi1c.pk005.j15 wdi1c Triticum aestivum c 321 G1749 BQ8036382.00E−12 Triticum monococcum WHE2839_H12_P23ZS Triticum monococcum v 321G1749 gi20160854 1.30E−15 Oryza sativa (japonica hypothetical protecultivar-group) 321 G1749 gi21740822 4.90E−14 Oryza sativaOSJNBa0042L16.10. 321 G1749 gi8809573 2.10E−13 Nicotiana sylvestrisethylene-responsive element binding 321 G1749 gi1208496 5.60E−13Nicotiana tabacum EREBP-3. 321 G1749 gi20340233 5.60E−13 Thellungiellahalophila ethylene responsive element bindi 321 G1749 gi3264767 1.20E−12Prunus armeniaca AP2 domain containing protein. 321 G1749 gi182661981.90E−12 Narcissus AP-2 domain containing pseudonarcissus protein. 321G1749 gi4099914 4.00E−12 Stylosanthes hamata ethylene-responsive elementbinding p 321 G1749 gi24940524 4.70E−12 Triticum aestivum ethyleneresponse element binding prote 321 G1749 gi18535580 5.10E−12Lycopersicon putative transcriptional esculentum activato 323 G1750BH459103 8.00E−61 Brassica oleracea BOGEX73TR BOGE Brassica oleraceagenomic 323 G1750 AP004902 7.00E−44 Lotus japonicus genomic DNA,chromosome 2, clone: LjT04G24, 323 G1750 AW685524 9.00E−39 Medicagotruncatula NF031C12NR1F1000 Nodulated root Medicag 323 G1750 LEU892574.00E−36 Lycopersicon DNA-binding protein Pti6 esculentum mRNA, comp 323G1750 BM886518 1.00E−35 Glycine max sam17f08.y1 Gm-c1068 Glycine maxcDNA clone SOY 323 G1750 AF058827 5.00E−32 Nicotiana tabacum TSI1 (Tsi1)mRNA, complete cds. 323 G1750 BQ873772 4.00E−30 Lactuca sativaQGI2I03.yg.ab1 QG_ABCDI lettuce salinas Lact 323 G1750 AP002835 1.00E−28Oryza sativa genomic DNA, chromosome 1, PAC clone: P0417G05. 323 G1750AAAA01000263 3.00E−28 Oryza sativa (indica ( ) scaffold000263cultivar-group) 323 G1750 BQ507568 1.00E−23 Solanum tuberosum EST614971Generation of a set of potato c 323 G1750 gi2213785 5.10E−35Lycopersicon Pti6. esculentum 323 G1750 gi8096469 1.50E−33 Oryza sativaSimilar to Arabidopsis thaliana chromosome 4 323 G1750 gi30658951.10E−32 Nicotiana tabacum TSI1. 323 G1750 gi7528276 1.90E−21Mesembryanthemum AP2-related transcription f crystallinum 323 G1750gi8571476 2.50E−21 Atriplex hortensis apetala2 domain-containingprotein. 323 G1750 gi8809575 1.30E−20 Nicotiana sylvestrisethylene-responsive element binding 323 G1750 gi17385636 1.70E−20Matricaria chamomilla ethylene-responsive element binding 323 G1750gi24060156 9.30E−20 Oryza sativa (japonica contains ESTs AU16cultivar-group) 323 G1750 gi4099914 1.50E−19 Stylosanthes hamataethylene-responsive element binding p 323 G1750 gi3264767 1.90E−19Prunus armeniaca AP2 domain containing protein. 325 G1756 BH5095551.00E−66 Brassica oleracea BOHIT47TF BOHI Brassica oleracea genomic 325G1756 BU837263 4.00E−42 Populus tremula x T096G05 Populus apica Populustremuloides 325 G1756 AW596933 2.00E−38 Glycine max sj84f07.y1 Gm-c1034Glycine max cDNA clone GENO 325 G1756 AV423663 7.00E−38 Lotus japonicusAV423663 Lotus japonicus young plants (two- 325 G1756 BI923414 6.00E−37Lycopersicon EST543319 tomato callus esculentum Lycopersico 325 G1756BM112869 6.00E−32 Solanum tuberosum EST560405 potato roots Solanumtuberosum 325 G1756 BF519892 7.00E−32 Medicago truncatula EST457357 DSILMedicago truncatula cDNA 325 G1756 AAAA01007990 5.00E−30 Oryza sativa(indica ( ) scaffold007990 cultivar-group) 325 G1756 AP004683 5.00E−30Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 325 G1756AW447931 3.00E−29 Triticum aestivum BRY_1082 BRY Triticum aestivum cDNAclone 325 G1756 gi11761072 3.00E−30 Oryza sativa hypothetical protein.325 G1756 gi4322940 1.20E−23 Nicotiana tabacum DNA-binding protein 2.325 G1756 gi4894963 2.20E−20 Avena sativa DNA-binding protein WRKY3. 325G1756 gi1432056 5.70E−20 Petroselinum crispum WRKY3. 325 G1756gi11993901 1.60E−19 Dactylis glomerata somatic embryogenesis relatedprotein. 325 G1756 gi13620227 2.00E−18 Lycopersicon hypotheticalprotein. esculentum 325 G1756 gi23305051 2.50E−18 Oryza sativa (indicaWRKY transcription f cultivar-group) 325 G1756 gi18158619 2.30E−17Retama raetam WRKY-like drought- induced protein. 325 G1756 gi228309857.60E−17 Oryza sativa (japonica WRKY transcription cultivar-group) 325G1756 gi7484759 1.70E−16 Cucumis sativus SP8 binding proteinhomolog-cucumber. 327 G1765 BF649854 7.00E−74 Medicago truncatulaNF085A08EC1F1055 Elicited cell culture 327 G1765 BI421877 2.00E−70Lycopersicon EST532543 tomato callus, esculentum TAMU Lycop 327 G1765BG511369 8.00E−60 Glycine max sad17a06.y1 Gm-c1074 Glycine max cDNAclone GEN 327 G1765 CA810372 9.00E−53 Vitis vinifera CA22LI05IF-C9CA22LI Vitis vinifera cDNA clo 327 G1765 BH519017 3.00E−47 Brassicaoleracea BOHHW49TR BOHH Brassica oleracea genomic 327 G1765 BQ5869912.00E−46 Beta vulgaris E012352-024-011-F06-SP6 MPIZ-ADIS-024-leaf Be 327G1765 BQ516602 9.00E−45 Solanum tuberosum EST624017 Generation of a setof potato c 327 G1765 BE034140 4.00E−44 Mesembryanthemum MG05E02 MGcrystallinum Mesembryanthemum c 327 G1765 AF509873 8.00E−44 Petunia xhybrida nam-like protein 10 (NH10) mRNA, complete 327 G1765 BU8838302.00E−43 Populus tremula x R002A08 Populus root Populus tremuloides 327G1765 gi20303588 1.60E−67 Oryza sativa (japonica putative NAM (no acultivar-group) 327 G1765 gi6175246 3.30E−47 Lycopersicon jasmonic acid2. esculentum 327 G1765 gi21105748 5.60E−45 Petunia x hybrida nam-likeprotein 10. 327 G1765 gi15148914 1.70E−43 Phaseolus vulgaris NAC domainprotein NAC2. 327 G1765 gi15528779 9.40E−43 Oryza sativa developmentregulation gene OsNAC4. 327 G1765 gi22597158 1.40E−41 Glycine max noapical meristem-like protein. 327 G1765 gi14485513 3.70E−41 Solanumtuberosum putative NAC domain protein. 327 G1765 gi4218537 1.00E−38Triticum sp. GRAB2 protein. 327 G1765 gi6732160 1.00E−38 Triticummonococcum unnamed protein product. 327 G1765 gi7716952 7.30E−36Medicago truncatula NAC1. 329 G1777 BQ996439 1.00E−120 Lactuca sativaQGG12N12.yg.ab1 QG_EFGHJ lettuce serriola La 329 G1777 BM9856391.00E−101 Thellungiella halophila 2_F04_T3 Ath Thellungiella halophil329 G1777 BM887188 4.00E−93 Glycine max sam35d01.y1 Gm-c1068 Glycine maxcDNA clone SOY 329 G1777 BM661323 5.00E−87 Zea mays 952046G05.y1 952-BMStissue from Walbot Lab (red 329 G1777 BU026535 9.00E−86 Helianthusannuus QHG17C11.yg.ab1 QH_EFGHJ sunflower RHA280 329 G1777 BH9987111.00E−84 Brassica oleracea oep82h07.g1 B. oleracea002 Brassica olerac329 G1777 AAAA01003274 1.00E−76 Oryza sativa (indica ( ) scaffold003274cultivar-group) 329 G1777 AC103891 2.00E−76 Oryza sativa chromosome 3clone OJ1175C11, *** SEQUENCING I 329 G1777 BG136684 1.00E−75Lycopersicon pennellii EST477126 wild tomato pollen Lycoper 329 G1777BG600834 4.00E−72 Solanum tuberosum EST505729 cSTS Solanum tuberosumcDNA clo 329 G1777 gi20330766 1.10E−199 Oryza sativa (japonica PutativeRING zinc cultivar-group) 329 G1777 gi1666171 4.90E−35 Nicotianaunknown. plumbaginifolia 329 G1777 gi1362039 0.76 Fragaria x ananassahypothetical protein (clone RJ39)-g 329 G1777 gi2244705 1 Nicotianaexcelsior gamma-thionin. 331 G1792 AI776626 5.00E−35 LycopersiconEST257726 tomato esculentum resistant, Cornell 331 G1792 BQ0457021.00E−32 Solanum tuberosum EST594820 P. infestans- challenged potato 331G1792 BM178875 7.00E−32 Glycine max saj60f01.y1 Gm-c1072 Glycine maxcDNA clone SOY 331 G1792 BF649790 1.00E−31 Medicago truncatulaNF084C07EC1F1052 Elicited cell culture 331 G1792 BZ020356 1.00E−30Brassica oleracea oeg04a10.g1 B. oleracea002 Brassica olerac 331 G1792BZ337899 3.00E−30 Sorghum bicolor ia91f11.b1 WGS-SbicolorF (JM107adapted met 331 G1792 AC025907 3.00E−30 Oryza sativa chromosome 10 clonenbxb0094K20, *** SEQUENCIN 331 G1792 AAAA01002491 3.00E−30 Oryza sativa(indica ( ) scaffold002491 cultivar-group) 331 G1792 BZ359367 8.00E−30Zea mays id72f11.b1 WGS-ZmaysF (JM107 adapted methyl filter 331 G1792AC137635 2.00E−27 Oryza sativa (japonica Genomic sequence forcultivar-group) 331 G1792 gi23452024 4.00E−26 Lycopersicon transcriptionfactor TSRF1. esculentum 331 G1792 gi1732406 2.10E−25 Nicotiana tabacumS25-XP1 DNA binding protein. 331 G1792 gi12597874 3.70E−25 Oryza sativaputative ethylene-responsive element binding 331 G1792 gi75282767.60E−25 Mesembryanthemum AP2-related transcription f crystallinum 331G1792 gi24060081 1.30E−23 Oryza sativa (japonica putative ethylenecultivar-group) 331 G1792 gi8980313 1.80E−23 Catharanthus roseusAP2-domain DNA-binding protein. 331 G1792 gi8809571 1.80E−23 Nicotianasylvestris ethylene-responsive element binding 331 G1792 gi173856361.20E−21 Matricaria chamomilla ethylene-responsive element binding 331G1792 gi21304712 3.10E−21 Glycine max ethylene-responsive elementbinding protein 1 331 G1792 gi8571476 1.10E−20 Atriplex hortensisapetala2 domain-containing protein. 333 G1793 CA783156 1.00E−121 Glycinemax sat20d05.y1 Gm-c1036 Glycine max cDNA clone SOY 333 G1793 AF3179041.00E−101 Brassica napus AP2/EREBP transcription factor BABY BOOM1 (B333 G1793 AY109146 2.00E−99 Zea mays PCO137288 mRNA sequence. 333 G1793AY062179 2.00E−99 Oryza sativa aintegumenta-like protein mRNA, completecds. 333 G1793 BQ864461 4.00E−91 Lactuca sativa QGC26M12.yg.ab1 QG_ABCDIlettuce salinas Lac 333 G1793 BJ178045 8.00E−89 Physcomitrella patensBJ178045 normalized ful subsp. patens 333 G1793 BF647766 3.00E−80Medicago truncatula NF025G09EC1F1071 Elicited cell culture 333 G1793AJ475492 1.00E−72 Hordeum vulgare AJ475492 S00008 Hordeum vulgare cDNAclone 333 G1793 BQ625052 9.00E−69 Citrus sinensis USDA-FP_02143 Ridgepineapple sweet orange 333 G1793 BJ312281 5.00E−65 Triticum aestivumBJ312281 Y. Ogihara unpublished cDNA libr 333 G1793 gi20161013 1.00E−107Oryza sativa (japonica putative ovule dev cultivar-group) 333 G1793gi21069053 5.70E−107 Brassica napus AP2/EREBP transcription factor BABYBOOM2. 333 G1793 gi21304227 1.50E−106 Oryza sativa ovule developmentaintegumenta-like protein 333 G1793 gi2652938 6.10E−97 Zea mays orf. 333G1793 gi13173164 5.60E−45 Pisum sativum APETAL2-like protein. 333 G1793gi11181612 3.20E−43 Picea abies APETALA2-related transcription factor 2.333 G1793 gi18476518 9.40E−43 Hordeum vulgare APETALA2-like protein. 333G1793 gi5081555 1.40E−41 Petunia x hybrida PHAP2A protein. 333 G1793gi21717332 9.70E−41 Malus x domestica transcription factor AHAP2. 333G1793 gi5360996 1.80E−34 Hyacinthus orientalis APETALA2 protein homologHAP2. 335 G1794 BH471138 4.00E−77 Brassica oleracea BOGTX58TF BOGTBrassica oleracea genomic 335 G1794 BU873559 2.00E−36 Populusbalsamifera Q056H03 Populus flow subsp. trichocarpa 335 G1794 AI4851752.00E−36 Lycopersicon EST243479 tomato ovary, esculentum TAMU Lycope 335G1794 BQ121959 3.00E−34 Solanum tuberosum EST607535 mixed potato tissuesSolanum tu 335 G1794 AC137522 3.00E−34 Medicago truncatula clonemth2-9h8, WORKING DRAFT SEQUENCE, 335 G1794 BU763025 3.00E−31 Glycinemax sas36c11.y1 Gm-c1080 Glycine max cDNA clone SOY 335 G1794 CA0155755.00E−31 Hordeum vulgare subsp. HT14L19r HT Hordeum vulgare vulgare 335G1794 BQ483206 6.00E−31 Triticum aestivum WHE3505_G10_M19ZS Wheatunstressed root c 335 G1794 AV428124 1.00E−30 Lotus japonicus AV428124Lotus japonicus young plants (two- 335 G1794 AP003286 9.00E−30 Oryzasativa chromosome 1 clone P0677H08, *** SEQUENCING IN 335 G1794gi20160854 4.80E−39 Oryza sativa (japonica hypothetical protecultivar-group) 335 G1794 gi21740822 2.50E−26 Oryza sativaOSJNBa0042L16.10. 335 G1794 gi10798644 9.80E−25 Nicotiana tabacum AP2domain-containing transcription fac 335 G1794 gi3342211 3.30E−24Lycopersicon Pti4. esculentum 335 G1794 gi8809575 2.60E−23 Nicotianasylvestris ethylene-responsive element binding 335 G1794 gi249405243.10E−23 Triticum aestivum ethylene response element binding prote 335G1794 gi24817250 9.50E−23 Cicer arietinum transcription factor EREBP-like protein. 335 G1794 gi3264767 1.70E−22 Prunus armeniaca AP2 domaincontaining protein. 335 G1794 gi20340233 1.70E−22 Thellungiellahalophila ethylene responsive element bindi 335 G1794 gi219080363.80E−22 Zea mays DRE binding factor 1. 337 G1804 BH496021 7.00E−87Brassica oleracea BOGJA54TR BOGJ Brassica oleracea genomic 337 G1804AF001453 2.00E−84 Helianthus annuus Dc3 promoter-binding factor-1(DPBF-1) mR 337 G1804 AF519804 5.00E−53 Triticum aestivum ABA responseelement binding factor (ABFB 337 G1804 AP003287 8.00E−53 Oryza sativachromosome 1 clone P0679C12, *** SEQUENCING IN 337 G1804 AAAA010014108.00E−53 Oryza sativa (indica ( ) scaffold001410 cultivar-group) 337G1804 VV1237992 7.00E−48 Vitis vinifera mRNA for putativeripening-related bZIP pro 337 G1804 AF369792 2.00E−46 Phaseolus vulgarisbZIP transcription factor 6 mRNA, comple 337 G1804 AB063648 2.00E−40Nicotiana tabacum mRNA for phi-2, complete cds. 337 G1804 AP0060579.00E−40 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 337G1804 AY110385 1.00E−38 Zea mays CL940_-1 mRNA sequence. 337 G1804gi2228771 1.40E−78 Helianthus annuus Dc3 promoter-binding factor-1. 337G1804 gi20161640 4.90E−47 Oryza sativa (japonica putative abscisiccultivar-group) 337 G1804 gi21693585 9.10E−43 Triticum aestivum ABAresponse element binding factor. 337 G1804 gi7406677 2.30E−40 Vitisvinifera putative ripening-related bZIP protein. 337 G1804 gi137751112.40E−37 Phaseolus vulgaris bZIP transcription factor 6. 337 G1804gi5821255 8.00E−35 Oryza sativa TRAB1. 337 G1804 gi14571808 3.10E−26Nicotiana tabacum phi-2. 337 G1804 gi1060935 1.30E−07 Zea mays mLIP15.337 G1804 gi2104677 1.70E−07 Vicia faba transcription factor. 337 G1804gi6018699 2.20E−07 Lycopersicon THY5 protein. esculentum 339 G1818BM065544 2.00E−29 Capsicum annuum KS07004F12 KS07 Capsicum annuum cDNA,mRNA 339 G1818 BU819346 7.00E−29 Populus tremula UA42BPF01 Populustremula cambium cDNA libr 339 G1818 AW776719 2.00E−28 Medicagotruncatula EST335784 DSIL Medicago truncatula cDNA 339 G1818 BG5916775.00E−28 Solanum tuberosum EST499519 P. infestans- challenged leaf So339 G1818 BI321875 3.00E−27 Glycine max saf52e11.y3 Gm-c1077 Glycine maxcDNA clone GEN 339 G1818 BE208917 1.00E−26 Citrus x paradisi GF-FV-P3F5Marsh grapefruit young flavedo 339 G1818 BG440805 1.00E−26 Gossypiumarboreum GA_Ea0010D12f Gossypium arboreum 7-10 d 339 G1818 BU5823244.00E−26 Zea mays 946188B03.y1 946-tassel primordium prepared by S 339G1818 BI127986 4.00E−26 Populus tremula x G069P33Y Populus camb Populustremuloides 339 G1818 AW093662 4.00E−26 Lycopersicon EST286842 tomatomixed esculentum elicitor, BT 339 G1818 gi5257260 4.60E−27 Oryza sativaSimilar to sequence of BAC F7G19 from Arabid 339 G1818 gi208044424.40E−13 Oryza sativa (japonica hypothetical prote cultivar-group) 339G1818 gi18481626 2.60E−07 Zea mays repressor protein. 339 G1818 gi1691950.95 Petunia x hybrida Major Cab protein. 339 G1818 gi1262851 0.98 Pinuspalustris type 2 light-harvesting chlorophyll a/b-b 339 G1818 gi225360100.99 Phaseolus coccineus LEC1-like protein. 341 G1820 AW776719 1.00E−43Medicago truncatula EST335784 DSIL Medicago truncatula cDNA 341 G1820BM065544 3.00E−40 Capsicum annuum KS07004F12 KS07 Capsicum annuum cDNA,mRNA 341 G1820 BG591677 4.00E−40 Solanum tuberosum EST499519 P.infestans- challenged leaf So 341 G1820 BI701620 1.00E−38 Glycine maxsai18a04.y1 Gm-c1053 Glycine max cDNA clone GEN 341 G1820 BQ4115973.00E−37 Gossypium arboreum GA_Ed0041B06f Gossypium arboreum 7-10 d 341G1820 BE208917 6.00E−37 Citrus x paradisi GF-FV-P3F5 Marsh grapefruityoung flavedo 341 G1820 BH725354 1.00E−36 Brassica oleracea BOHVO37TFBO_2_3_KB Brassica oleracea gen 341 G1820 AW093662 9.00E−36 LycopersiconEST286842 tomato mixed esculentum elicitor, BT 341 G1820 BU8193464.00E−35 Populus tremula UA42BPF01 Populus tremula cambium cDNA libr 341G1820 AAAA01002977 3.00E−34 Oryza sativa (indica ( ) scaffold002977cultivar-group) 341 G1820 gi5257260 1.40E−34 Oryza sativa Similar tosequence of BAC F7G19 from Arabid 341 G1820 gi20804442 1.70E−15 Oryzasativa (japonica hypothetical prote cultivar-group) 341 G1820 gi184816266.30E−08 Zea mays repressor protein. 341 G1820 gi297871 0.39 Picea abieshistone H2A. 341 G1820 gi297887 0.41 Daucus carota glycine rich protein.341 G1820 gi2130105 0.54 Triticum aestivum histone H2A.4-wheat. 341G1820 gi6782438 0.74 Nicotiana glauca glycine-rich protein. 341 G1820gi15214035 0.98 Cicer arietinum HISTONE H2A. 341 G1820 gi2317760 0.98Pinus taeda H2A homolog. 341 G1820 gi1173628 0.99 Phalaenopsis sp.glycine-rich protein. SM9108 343 G1836 BI701620 7.00E−35 Glycine maxsai18a04.y1 Gm-c1053 Glycine max cDNA clone GEN 343 G1836 AW7767192.00E−33 Medicago truncatula EST335784 DSIL Medicago truncatula cDNA 343G1836 BQ411597 2.00E−33 Gossypium arboreum GA_Ed0041B06f Gossypiumarboreum 7-10 d 343 G1836 BM065544 2.00E−32 Capsicum annuum KS07004F12KS07 Capsicum annuum cDNA, mRNA 343 G1836 BG591677 3.00E−31 Solanumtuberosum EST499519 P. infestans- challenged leaf So 343 G1836 BU8193466.00E−31 Populus tremula UA42BPF01 Populus tremula cambium cDNA libr 343G1836 BH725354 4.00E−30 Brassica oleracea BOHVO37TF BO_2_3_KB Brassicaoleracea gen 343 G1836 BE208917 6.00E−30 Citrus x paradisi GF-FV-P3F5Marsh grapefruit young flavedo 343 G1836 AAAA01024926 5.00E−29 Oryzasativa (indica ( ) scaffold024926 cultivar-group) 343 G1836 AW0936629.00E−29 Lycopersicon EST286842 tomato mixed esculentum elicitor, BT 343G1836 gi5257260 2.10E−29 Oryza sativa Similar to sequence of BAC F7G19from Arabid 343 G1836 gi20804442 6.30E−16 Oryza sativa (japonicahypothetical prote cultivar-group) 343 G1836 gi18481626 2.00E−06 Zeamays repressor protein. 343 G1836 gi18539425 0.84 Pinus sylvestrisputative malate dehydrogenase. 343 G1836 gi122084 1 Hordeum vulgareHistone H3. 343 G1836 gi225348 1 Hordeum vulgare subsp. histone H3.vulgare 345 G1838 AF317904 2.00E−98 Brassica napus AP2/EREBPtranscription factor BABY BOOM1 (B 345 G1838 CA783156 7.00E−97 Glycinemax sat20d05.y1 Gm-c1036 Glycine max cDNA clone SOY 345 G1838 AY1091466.00E−96 Zea mays PCO137288 mRNA sequence. 345 G1838 AY062179 2.00E−93Oryza sativa aintegumenta-like protein mRNA, complete cds. 345 G1838BJ178045 3.00E−84 Physcomitrella patens BJ178045 normalized ful subsp.patens 345 G1838 BQ864461 2.00E−83 Lactuca sativa QGC26M12.yg.ab1QG_ABCDI lettuce salinas Lac 345 G1838 BF647766 5.00E−73 Medicagotruncatula NF025G09EC1F1071 Elicited cell culture 345 G1838 AJ4754923.00E−69 Hordeum vulgare AJ475492 S00008 Hordeum vulgare cDNA clone 345G1838 BQ625052 6.00E−69 Citrus sinensis USDA-FP_02143 Ridge pineapplesweet orange 345 G1838 BJ312281 4.00E−60 Triticum aestivum BJ312281 Y.Ogihara unpublished cDNA libr 345 G1838 gi21069051 3.00E−100 Brassicanapus AP2/EREBP transcription factor BABY BOOM1. 345 G1838 gi213042251.30E−95 Oryza sativa aintegumenta-like protein. 345 G1838 gi201610133.00E−91 Oryza sativa (japonica putative ovule dev cultivar-group) 345G1838 gi2652938 2.50E−90 Zea mays orf. 345 G1838 gi13173164 1.10E−51Pisum sativum APETAL2-like protein. 345 G1838 gi21717332 1.10E−46 Malusx domestica transcription factor AHAP2. 345 G1838 gi5081557 1.50E−44Petunia x hybrida PHAP2B protein. 345 G1838 gi18476518 9.40E−43 Hordeumvulgare APETALA2-like protein. 345 G1838 gi11181612 9.90E−42 Picea abiesAPETALA2-related transcription factor 2. 345 G1838 gi5360996 3.60E−34Hyacinthus orientalis APETALA2 protein homolog HAP2. 347 G1841 BI4218952.00E−37 Lycopersicon EST532561 tomato callus, esculentum TAMU Lycop 347G1841 BU873559 3.00E−36 Populus balsamifera Q056H03 Populus flow subsp.trichocarpa 347 G1841 AC120527 6.00E−35 Oryza sativa chromosome 11 cloneOSJNBa0011J22, *** SEQUENC 347 G1841 AAAA01002409 2.00E−34 Oryza sativa(indica ( ) scaffold002409 cultivar-group) 347 G1841 BE429439 7.00E−34Triticum aestivum TAS000.B08R990618 ITEC TAS Wheat cDNA Lib 347 G1841AW685799 2.00E−32 Medicago truncatula NF030D09NR1F1000 Nodulated rootMedicag 347 G1841 BE494041 5.00E−32 Secale cereale WHE1277_B09_D17ZSSecale cereale anther cDNA 347 G1841 BU763025 1.00E−31 Glycine maxsas36c11.y1 Gm-c1080 Glycine max cDNA clone SOY 347 G1841 CA0155754.00E−31 Hordeum vulgare subsp. HT14L19r HT Hordeum vulgare vulgare 347G1841 AV428124 8.00E−30 Lotus japonicus AV428124 Lotus japonicus youngplants (two- 347 G1841 gi20160854 4.00E−37 Oryza sativa (japonicahypothetical prote cultivar-group) 347 G1841 gi10798644 2.80E−27Nicotiana tabacum AP2 domain-containing transcription fac 347 G1841gi21740822 2.50E−26 Oryza sativa OSJNBa0042L16.10. 347 G1841 gi220740464.20E−24 Lycopersicon transcription factor JERF1. esculentum 347 G1841gi24817250 4.20E−24 Cicer arietinum transcription factor EREBP- likeprotein. 347 G1841 gi1688233 5.40E−24 Solanum tuberosum DNA bindingprotein homolog. 347 G1841 gi3264767 1.10E−23 Prunus armeniaca AP2domain containing protein. 347 G1841 gi18496063 3.00E−23 Fagus sylvaticaethylene responsive element binding prote 347 G1841 gi24940524 1.00E−22Triticum aestivum ethylene response element binding prote 347 G1841gi20340233 2.70E−22 Thellungiella halophila ethylene responsive elementbindi 349 G1842 AY036888 5.00E−56 Brassica napus MADS-box protein (FLC1)mRNA, complete cds. 349 G1842 BG544805 2.00E−37 Brassica rapa subsp.E2809 Chinese cabbage pekinensis etiol 349 G1842 BG596731 7.00E−36Solanum tuberosum EST495409 cSTS Solanum tuberosum cDNA clo 349 G1842AW219962 9.00E−36 Lycopersicon EST302445 tomato root esculentumduring/after 349 G1842 BM436799 4.00E−34 Vitis vinifera VVA010B05_53181An expressed sequence tag da 349 G1842 BQ868455 2.00E−30 Lactuca sativaQGD14A13.yg.ab1 QG_ABCDI lettuce salinas Lac 349 G1842 BI957545 1.00E−29Hordeum vulgare HVSMEn0010B09f Hordeum vulgare rachis EST1 349 G1842BJ213269 2.00E−29 Triticum aestivum BJ213269 Y. Ogihara unpublished cDNAlibr 349 G1842 AI900863 4.00E−29 Glycine max sb95d06.y1 Gm-c1012 Glycinemax cDNA clone GENO 349 G1842 AF112150 5.00E−29 Zea mays MADS boxprotein 3 (mads3) mRNA, complete cds. 349 G1842 gi17933450 4.80E−55Brassica napus MADS-box protein. 349 G1842 gi1483232 1.10E−30 Betulapendula MADS5 protein. 349 G1842 gi9367313 1.40E−30 Hordeum vulgareMADS-box protein 8. 349 G1842 gi6469345 1.80E−30 Brassica rapa subsp.DNA-binding protein. pekinensis 349 G1842 gi12002141 3.00E−30 Zea maysMADS box protein 3. 349 G1842 gi11037010 6.30E−30 Eucalyptus globulusMADS-box protein EAP2S. 349 G1842 gi1561784 6.30E−30 Brassica oleraceahomeotic protein boiCAL. 349 G1842 gi4204234 8.00E−30 Lolium temulentumMADS-box protein 2. 349 G1842 gi13446154 1.70E−29 Pisum sativum MADS-boxtranscription factor. 349 G1842 gi21070923 1.70E−29 Oryza sativa(japonica AP1-like MADS-box cultivar-group) 351 G1843 AY036889 5.00E−56Brassica napus MADS-box protein (FLC2) mRNA, complete cds. 351 G1843BG596731 3.00E−35 Solanum tuberosum EST495409 cSTS Solanum tuberosumcDNA clo 351 G1843 BG544805 4.00E−35 Brassica rapa subsp. E2809 Chinesecabbage pekinensis etiol 351 G1843 AW219962 2.00E−34 LycopersiconEST302445 tomato root esculentum during/after 351 G1843 BM4367994.00E−34 Vitis vinifera VVA010B05_53181 An expressed sequence tag da 351G1843 BQ850592 4.00E−32 Lactuca sativa QGB13A16.yg.ab1 QG_ABCDI lettucesalinas Lac 351 G1843 BU875165 8.00E−32 Populus balsamifera V003A12Populus flow subsp. trichocarpa 351 G1843 BU887610 9.00E−31 Populustremula x R064B01 Populus root Populus tremuloides 351 G1843 AF0353793.00E−30 Lolium temulentum MADS-box protein 2 (MADS2) mRNA, alternat 351G1843 AY040247 6.00E−30 Antirrhinum majus MADS-box transcription factorDEFH28 mRNA 351 G1843 gi17933452 2.30E−55 Brassica napus MADS-boxprotein. 351 G1843 gi21070923 7.80E−32 Oryza sativa (japonica AP1-likeMADS-box cultivar-group) 351 G1843 gi16874557 1.60E−31 Antirrhinum majusMADS-box transcription factor DEFH28. 351 G1843 gi4204234 2.60E−31Lolium temulentum MADS-box protein 2. 351 G1843 gi7592642 2.60E−31 Oryzasativa AP1-like MADS box protein. 351 G1843 gi9367313 7.00E−31 Hordeumvulgare MADS-box protein 8. 351 G1843 gi3688589 4.90E−30 Triticumaestivum MADS box transcription factor. 351 G1843 gi6467974 1.00E−29Dendrobium grex MADS box protein Madame Thong-In DOMADS2. 351 G1843gi1483232 1.30E−29 Betula pendula MADS5 protein. 351 G1843 gi133840681.70E−29 Petunia x hybrida MADS-box transcription factor FBP29. 353G1852 AAAA01018591 1.00E−135 Oryza sativa (indica ( ) scaffold018591cultivar-group) 353 G1852 AF220204 1.00E−129 Malus domestica unknownmRNA. 353 G1852 BQ507509 1.00E−119 Solanum tuberosum EST614924Generation of a set of potato c 353 G1852 BM412458 1.00E−114Lycopersicon EST586785 tomato breaker esculentum fruit Lyco 353 G1852AY104480 1.00E−113 Zea mays PCO099563 mRNA sequence. 353 G1852 BG5817051.00E−108 Medicago truncatula EST483440 GVN Medicago truncatula cDNA 353G1852 BF009089 1.00E−102 Glycine max ss73d04.y1 Gm-c1062 Glycine maxcDNA clone GENO 353 G1852 AC087192 1.00E−101 Oryza sativa chromosome 10clone OSJNBa0005K07, *** SEQUENC 353 G1852 BU013091 1.00E−100 Lactucasativa QGJ3L13.yg.ab1 QG_EFGHJ lettuce serriola Lac 353 G1852 BG4459229.00E−99 Gossypium arboreum GA_Ea0030A23f Gossypium arboreum 7-10 d 353G1852 gi24413975 8.10E−124 Oryza sativa (japonica hypothetical protecultivar-group) 353 G1852 gi6752888 2.70E−123 Malus x domestica unknown.353 G1852 gi18071395 1.20E−122 Oryza sativa hypothetical protein. 353G1852 gi18419598 1.30E−22 Narcissus putative methyltransferasepseudonarcissus prot 353 G1852 gi20218829 6.60E−16 Pinus pinasterhypothetical protein. 353 G1852 gi15144514 0.089 Lycopersicon unknown.esculentum 353 G1852 gi498042 0.23 Senecio odorus ORF. 353 G1852gi4432741 0.69 Dioscorea tenuipes phosphoglucose isomerase. 353 G1852gi1399380 0.81 Glycine max S-adenosyl-L- methionine:delta24-sterol-C-meth 355 G1863 BH582941 4.00E−61 Brassica oleracea BOHOL42TF BOHOBrassica oleracea genomic 355 G1863 AF201895 1.00E−34 Oryza sativagrowth-regulating factor 1 (GRF1) mRNA, comple 355 G1863 BM4048722.00E−34 Solanum tuberosum EST579199 potato roots Solanum tuberosum 355G1863 AW981431 8.00E−34 Medicago truncatula EST392584 DSIL Medicagotruncatula cDNA 355 G1863 BI786182 1.00E−33 Glycine max sai33h05.y1Gm-c1065 Glycine max cDNA clone GEN 355 G1863 BQ852906 3.00E−33 Lactucasativa QGB19E24.yg.ab1 QG_ABCDI lettuce salinas Lac 355 G1863 AW4422271.00E−32 Lycopersicon EST311623 tomato fruit red esculentum ripe, TA 355G1863 CA029723 3.00E−32 Hordeum vulgare subsp. HX05A15r HX Hordeumvulgare vulgare 355 G1863 AP005538 6.00E−32 Oryza sativa (japonica ( )chromosome 2 clo cultivar-group) 355 G1863 AAAA01004865 1.00E−31 Oryzasativa (indica ( ) scaffold004865 cultivar-group) 355 G1863 gi65731491.90E−39 Oryza sativa growth-regulating factor 1. 355 G1863 gi183900991.20E−37 Sorghum bicolor putative growth-regulating factor 1. 355 G1863gi24413958 1.20E−33 Oryza sativa (japonica putative growth-recultivar-group) 355 G1863 gi19171209 0.12 Lycopersicon viroidRNA-binding protein. esculentum 355 G1863 gi7008009 0.67 Pisum sativumPsAD1. 355 G1863 gi1061308 0.79 Zea mays Dof3 gene. 355 G1863 gi21298290.96 Glycine max heat shock transcription factor HSF29-soybe 355 G1863gi4680184 0.99 Oryza sativa (indica unknown. cultivar-group) 355 G1863gi12655953 1 Brassica rapa luminidependens. 355 G1863 gi3790264 1Triticum aestivum PBF protein. 357 G1880 BI265111 1.00E−75 Medicagotruncatula NF078A11IN1F1085 Insect herbivory Medic 357 G1880 BJ1922018.00E−75 Physcomitrella patens BJ192201 normalized ful subsp. patens 357G1880 BH714361 3.00E−73 Brassica oleracea BOMMJ59TR BO_2_3_KB Brassicaoleracea gen 357 G1880 BI972592 1.00E−71 Glycine max sai80b06.y1Gm-c1065 Glycine max cDNA clone GEN 357 G1880 AP005381 2.00E−71 Oryzasativa (japonica ( ) chromosome 8 clo cultivar-group) 357 G1880AAAA01002232 2.00E−69 Oryza sativa (indica ( ) scaffold002232cultivar-group) 357 G1880 BM063853 4.00E−61 Capsicum annuum KS01060C10KS01 Capsicum annuum cDNA, mRNA 357 G1880 BU039744 1.00E−60 Prunuspersica PP_LEa0003M02f Peach developing fruit mesoca 357 G1880 BM4077093.00E−60 Solanum tuberosum EST582036 potato roots Solanum tuberosum 357G1880 BF050813 7.00E−60 Lycopersicon EST435971 tomato esculentumdeveloping/immatur 357 G1880 gi9858780 1.50E−58 Lycopersicon BAC19.12.esculentum 357 G1880 gi10934090 1.20E−57 Oryza sativa putative zincfinger protein. 357 G1880 gi563623 2.20E−57 Solanum tuberosum putativeDNA/RNA binding protein. 357 G1880 gi3170601 3.30E−57 Zea mays zincfinger protein ID1. 357 G1880 gi20160482 1.40E−56 Oryza sativa (japonicazinc finger protei cultivar-group) 357 G1880 gi18376601 4.40E−12 Glycinemax WIP1 protein. 357 G1880 gi2346988 0.059 Petunia x hybrida ZPT4-4.357 G1880 gi1076538 0.1 Pisum sativum gibberellin-responsive ovarianprotein G14 357 G1880 gi3129939 0.81 Cicer arietinum hypotheticalprotein. 357 G1880 gi12585428 0.91 Nicotiana tabacum Vacuolar ATPsynthase subunit G 1 (V-AT 359 G1895 BH418383 5.00E−94 Brassica oleraceaBOHQS10TR BOHQ Brassica oleracea genomic 359 G1895 AC073556 7.00E−35Oryza sativa chromosome unknown clone OSJNBa0091P11, *** SE 359 G1895D45066 3.00E−34 Cucurbita maxima mRNA for AOBP (ascorbate oxidasepromoter- 359 G1895 BQ488386 2.00E−33 Beta vulgaris43-E8885-006-003-F11-T3 Sugar beet MPIZ-ADIS- 359 G1895 BF6494989.00E−33 Medicago truncatula NF079C08EC1F1065 Elicited cell culture 359G1895 BQ860203 3.00E−32 Lactuca sativa QGC15B22.yg.ab1 QG_ABCDI lettucesalinas Lac 359 G1895 HVU312330 4.00E−32 Hordeum vulgare subsp. Hordeumvulgare partial dof vulgare 359 G1895 AW931465 3.00E−31 LycopersiconEST357308 tomato fruit esculentum mature green 359 G1895 AAAA010076354.00E−31 Oryza sativa (indica ( ) scaffold007635 cultivar-group) 359G1895 CA783807 1.00E−30 Glycine max sat57f01.y1 Gm-c1056 Glycine maxcDNA clone SOY 359 G1895 gi19071625 1.80E−41 Oryza sativa (japonicaputative zinc fing cultivar-group) 359 G1895 gi7242908 1.40E−40 Oryzasativa ESTs C23582(S11122), AU056531 (S20663) corresp 359 G1895gi1669341 1.50E−39 Cucurbita maxima AOBP (ascorbate oxidasepromoter-binding 359 G1895 gi21538791 4.90E−30 Hordeum vulgare subsp.dof zinc finger protein. vulgare 359 G1895 gi3929325 6.90E−24 Dendrobiumgrex putative DNA-binding prot Madame Thong-In 359 G1895 gi13600781.10E−23 Nicotiana tabacum Zn finger protein. 359 G1895 gi60920161.30E−22 Pisum sativum elicitor-responsive Dof protein ERDP. 359 G1895gi7688355 3.40E−22 Solanum tuberosum Dof zinc finger protein. 359 G1895gi1061306 9.00E−22 Zea mays Dof2. 359 G1895 gi3790264 3.90E−21 Triticumaestivum PBF protein. 361 G1902 BH516623 8.00E−87 Brassica oleraceaBOGHO31TR BOGH Brassica oleracea genomic 361 G1902 BE610227 3.00E−40Glycine max sq51e07.y1 Gm-c1019 Glycine max cDNA clone GENO 361 G1902BE433484 2.00E−38 Lycopersicon EST400013 tomato breaker esculentumfruit, TIG 361 G1902 BQ790994 3.00E−38 Brassica rapa subsp. E4860Chinese cabbage pekinensis etiol 361 G1902 BQ505729 3.00E−37 Solanumtuberosum EST613144 Generation of a set of potato c 361 G1902 BG4543381.00E−35 Medicago truncatula NF113E12LF1F1088 Developing leaf Medica 361G1902 BU832216 1.00E−31 Populus tremula x T030H07 Populus apica Populustremuloides 361 G1902 BM066503 6.00E−30 Capsicum annuum KS07015B04 KS07Capsicum annuum cDNA, mRNA 361 G1902 AC133003 7.00E−30 Oryza sativa(japonica ( ) chromosome 3 clo cultivar-group) 361 G1902 AW3981401.00E−29 Lycopersicon pennellii EST298023 L. pennellii trichome, Cor 361G1902 gi4996640 3.70E−31 Oryza sativa Dof zinc finger protein. 361 G1902gi3341468 1.80E−30 Nicotiana tabacum Dof zinc finger protein. 361 G1902gi3790264 2.60E−30 Triticum aestivum PBF protein. 361 G1902 gi193872523.30E−30 Oryza sativa (japonica putative zinc-fing cultivar-group) 361G1902 gi21538793 1.30E−29 Hordeum vulgare subsp. dof zinc fingerprotein. vulgare 361 G1902 gi3777436 1.80E−29 Hordeum vulgare DNAbinding protein. 361 G1902 gi6092016 2.10E−29 Pisum sativumelicitor-responsive Dof protein ERDP. 361 G1902 gi1061308 6.60E−28 Zeamays Dof3 gene. 361 G1902 gi7688355 1.70E−27 Solanum tuberosum Dof zincfinger protein. 361 G1902 gi1669341 1.00E−22 Cucurbita maxima AOBP(ascorbate oxidase promoter-binding 363 G1903 BH590326 1.00E−111Brassica oleracea BOGGK32TR BOGG Brassica oleracea genomic 363 G1903AC073556 2.00E−41 Oryza sativa chromosome unknown clone OSJNBa0091P11,*** SE 363 G1903 D45066 1.00E−39 Cucurbita maxima mRNA for AOBP(ascorbate oxidase promoter- 363 G1903 HVU312330 3.00E−37 Hordeumvulgare subsp. Hordeum vulgare partial dof vulgare 363 G1903 AP0051673.00E−35 Oryza sativa (japonica ( ) chromosome 7 clo cultivar-group) 363G1903 BQ860203 3.00E−35 Lactuca sativa QGC15B22.yg.ab1 QG_ABCDI lettucesalinas Lac 363 G1903 BI934963 3.00E−35 Lycopersicon EST554852 tomatoflower, esculentum anthesis L 363 G1903 AAAA01004298 4.00E−35 Oryzasativa (indica ( ) scaffold004298 cultivar-group) 363 G1903 BF6494989.00E−35 Medicago truncatula NF079C08EC1F1065 Elicited cell culture 363G1903 CA484955 2.00E−33 Triticum aestivum WHE4312_F07_L14ZS Wheatmeiotic anther cD 363 G1903 gi19071625 6.70E−47 Oryza sativa (japonicaputative zinc fing cultivar-group) 363 G1903 gi7242908 3.20E−43 Oryzasativa ESTs C23582(S11122), AU056531 (S20663) corresp 363 G1903gi1669341 8.50E−43 Cucurbita maxima AOBP (ascorbate oxidasepromoter-binding 363 G1903 gi21538791 1.90E−39 Hordeum vulgare subsp.dof zinc finger protein. vulgare 363 G1903 gi1360084 6.20E−26 Nicotianatabacum Zn finger protein. 363 G1903 gi3790264 9.20E−26 Triticumaestivum PBF protein. 363 G1903 gi2393775 7.10E−25 Zea mays prolamin boxbinding factor. 363 G1903 gi7688355 4.20E−24 Solanum tuberosum Dof zincfinger protein. 363 G1903 gi6092016 5.50E−24 Pisum sativumelicitor-responsive Dof protein ERDP. 363 G1903 gi3929325 9.50E−24Dendrobium grex putative DNA-binding prot Madame Thong-In 365 G1919BH997456 3.00E−39 Brassica oleracea oef07e04.b1 B. oleracea002 Brassicaolerac 365 G1919 AP005090 7.00E−28 Oryza sativa (japonica ( ) chromosome9 clo cultivar-group) 365 G1919 AAAA01013304 2.00E−27 Oryza sativa(indica ( ) scaffold013304 cultivar-group) 365 G1919 AC126012 3.00E−26Medicago truncatula clone mth2-27p4, WORKING DRAFT SEQUENCE 365 G1919NPY09105 1.00E−23 Nicotiana N. plumbaginifolia mRNA plumbaginifolia forunknow 365 G1919 BU000353 1.00E−22 Lactuca sativa QGG24J16.yg.ab1QG_EFGHJ lettuce serriola La 365 G1919 AV914826 1.00E−20 Hordeum vulgaresubsp. AV914826 K. Sato vulgare unpublished 365 G1919 AW704699 4.00E−18Glycine max sk39d07.y1 Gm-c1028 Glycine max cDNA clone GENO 365 G1919BE415217 4.00E−17 Triticum aestivum MWL025.F02F000208 ITEC MWL WheatRoot Lib 365 G1919 BF587440 6.00E−17 Sorghum propinquumFM1_36_D07.b1_A003 Floral-Induced Merist 365 G1919 gi1666171 2.10E−25Nicotiana unknown. plumbaginifolia 365 G1919 gi20330766 1.00E−17 Oryzasativa (japonica Putative RING zinc cultivar-group) 365 G1919 gi5064691.80E−05 Nicotiana tabacum unnamed protein product. 365 G1919 gi1199510.78 Phytolacca esculenta FERREDOXIN II. 365 G1919 gi2914662 0.83Chlorella' fusca Ferredoxin Oxidized Form From Chlorella 365 G1919gi11344770 0.87 Phaseolus vulgaris alpha-amylase inhibitor. 365 G1919gi119950 1 Phytolacca americana Ferredoxin II. 367 G1927 AF5098701.00E−104 Petunia x hybrida nam-like protein 7 (NH7) mRNA, complete c367 G1927 BQ864249 4.00E−74 Lactuca sativa QGC26D03.yg.ab1 QG_ABCDIlettuce salinas Lac 367 G1927 BG350410 3.00E−73 Solanum tuberosum 091B07Mature tuber lambda ZAP Solanum tu 367 G1927 BU863110 8.00E−70 Populustremula x S024B04 Populus imbib Populus tremuloides 367 G1927 AW7364147.00E−59 Medicago truncatula EST332428 KV3 Medicago truncatula cDNA 367G1927 BJ481205 2.00E−56 Hordeum vulgare subsp. BJ481205 K. Sato unpublisspontaneum 367 G1927 BF066070 2.00E−55 Hordeum vulgare HV_CEb0014M06fHordeum vulgare seedling gre 367 G1927 BG159075 4.00E−55 Sorghumpropinquum RHIZ2_17_E07.b1_A003 Rhizome2 (RHIZ2) So 367 G1927 BU0253101.00E−53 Helianthus annuus QHF8N06.yg.ab1 QH_EFGHJ sunflower RHA280 367G1927 BJ234447 4.00E−53 Triticum aestivum BJ234447 Y. Ogiharaunpublished cDNA libr 367 G1927 gi21105742 6.70E−102 Petunia x hybridanam-like protein 7. 367 G1927 gi7716952 3.20E−49 Medicago truncatulaNAC1. 367 G1927 gi19225018 8.60E−47 Oryza sativa (japonica putative NAM(no a cultivar-group) 367 G1927 gi6730946 9.60E−44 Oryza sativa OsNAC8protein. 367 G1927 gi15148914 5.40E−40 Phaseolus vulgaris NAC domainprotein NAC2. 367 G1927 gi6175246 8.70E−40 Lycopersicon jasmonic acid 2.esculentum 367 G1927 gi22597158 3.40E−38 Glycine max no apicalmeristem-like protein. 367 G1927 gi4218537 6.40E−37 Triticum sp. GRAB2protein. 367 G1927 gi6732160 6.40E−37 Triticum monococcum unnamedprotein product. 367 G1927 gi14485513 1.30E−35 Solanum tuberosumputative NAC domain protein. 369 G1930 BU025988 5.00E−88 Helianthusannuus QHG12J17.yg.ab1 QH_EFGHJ sunflower RHA280 369 G1930 AP0034508.00E−80 Oryza sativa chromosome 1 clone P0034C09, *** SEQUENCING IN 369G1930 AC135925 7.00E−79 Oryza sativa (japonica ( ) chromosome 5 clocultivar-group) 369 G1930 AAAA01000997 3.00E−78 Oryza sativa (indica ( )scaffold000997 cultivar-group) 369 G1930 BU994579 1.00E−65 Hordeumvulgare subsp. HM07I08r HM Hordeum vulgare vulgare 369 G1930 BQ4056981.00E−65 Gossypium arboreum GA_Ed0085H02f Gossypium arboreum 7-10 d 369G1930 BF520598 1.00E−64 Medicago truncatula EST458071 DSIL Medicagotruncatula cDNA 369 G1930 BZ015521 1.00E−64 Brassica oleraceaoeg86a05.g1 B. oleracea002 Brassica olerac 369 G1930 BF424857 2.00E−58Glycine max su59h03.y1 Gm-c1069 Glycine max cDNA clone GENO 369 G1930BU870896 1.00E−56 Populus balsamifera Q019F06 Populus flow subsp.trichocarpa 369 G1930 gi18565433 4.10E−74 Oryza sativa (japonicaDNA-binding protei cultivar-group) 369 G1930 gi12328560 1.80E−71 Oryzasativa putative DNA binding protein RAV2. 369 G1930 gi10798644 1.40E−13Nicotiana tabacum AP2 domain-containing transcription fac 369 G1930gi20340233 5.10E−11 Thellungiella halophila ethylene responsive elementbindi 369 G1930 gi4099921 1.30E−10 Stylosanthes hamata EREBP-3 homolog.369 G1930 gi18496063 1.60E−10 Fagus sylvatica ethylene responsiveelement binding prote 369 G1930 gi22074046 2.10E−10 Lycopersicontranscription factor JERF1. esculentum 369 G1930 gi3264767 2.30E−10Prunus armeniaca AP2 domain containing protein. 369 G1930 gi182661981.10E−09 Narcissus AP-2 domain containing pseudonarcissus protein. 369G1930 gi24940524 1.10E−09 Triticum aestivum ethylene response elementbinding prote 371 G1936 AX540653 1.00E−139 Zea mays Sequence 9 fromPatent WO0240688. 371 G1936 BH735681 4.00E−45 Brassica oleraceaBOHTG58TR BO_2_3_KB Brassica oleracea gen 371 G1936 AW279046 2.00E−28Glycine max sg07b03.y1 Gm-c1019 Glycine max cDNA clone GENO 371 G1936BQ874162 2.00E−26 Lactuca sativa QGI4J06.yg.ab1 QG_ABCDI lettuce salinasLact 371 G1936 BG645784 5.00E−26 Medicago truncatula EST507403 KV3Medicago truncatula cDNA 371 G1936 AP004223 5.00E−26 Oryza sativa(japonica ( ) genomic DNA, chr cultivar-group) 371 G1936 AW2190902.00E−23 Lycopersicon EST301572 tomato root esculentum during/after 371G1936 BQ118395 5.00E−23 Solanum tuberosum EST603971 mixed potato tissuesSolanum tu 371 G1936 CA816557 6.00E−23 Vitis vinifera CA12EI303IVF_H11Cabernet Sauvignon Leaf-C 371 G1936 BG445379 3.00E−22 Gossypium arboreumGA_Ea0027O21f Gossypium arboreum 7-10 d 371 G1936 gi20975251 7.50E−23Oryza sativa (japonica transcription fact cultivar-group) 371 G1936gi2580440 2.20E−21 Oryza sativa PCF2. 371 G1936 gi5731257 5.00E−21Gossypium hirsutum auxin-induced basic helix- loop-helix t 371 G1936gi6358622 0.00035 Digitalis purpurea cyc4 protein. 371 G1936 gi63586250.00035 Misopates orontium cyc4 protein. 371 G1936 gi6358621 0.00061Antirrhinum majus cyc4 protein. subsp. cirrhigerum 371 G1936 gi63586230.00061 Antirrhinum graniticum cyc4 protein. 371 G1936 gi6466188 0.00085Antirrhinum majus flower asymmetry protein DICHOTOMA. 371 G1936gi12002867 0.0036 Lycopersicon cycloidea. esculentum 371 G1936 gi63585510.0092 Antirrhinum majus cyc1A protein. subsp. linkianum 373 G1944BU926769 1.00E−86 Glycine max sas91d09.y1 Gm-c1036 Glycine max cDNAclone SOY 373 G1944 BU814921 8.00E−73 Populus tremula x N034H11 Populusbark Populus tremuloides 373 G1944 BG589060 8.00E−70 Medicago truncatulaEST490869 MHRP- Medicago truncatula cDN 373 G1944 BG441060 1.00E−64Gossypium arboreum GA_Ea0011I19f Gossypium arboreum 7-10 d 373 G1944BI139442 3.00E−64 Populus balsamifera F131P74Y Populus flo subsp.trichocarpa 373 G1944 BG643949 5.00E−59 Lycopersicon EST512143 tomatoesculentum shoot/meristem Lyc 373 G1944 AU289368 2.00E−58 Zinnia elegansAU289368 zinnia cultured mesophyll cell equa 373 G1944 BQ868100 3.00E−52Lactuca sativa QGD13A19.yg.ab1 QG_ABCDI lettuce salinas Lac 373 G1944BU892499 2.00E−50 Populus tremula P064F04 Populus petioles cDNA libraryPopul 373 G1944 AV425818 1.00E−48 Lotus japonicus AV425818 Lotusjaponicus young plants (two- 373 G1944 gi12643044 7.80E−58 Oryza sativaputative AT-Hook DNA- binding protein. 373 G1944 gi2213536 4.40E−45Pisum sativum DNA-binding protein PD1. 373 G1944 gi4165183 3.20E−41Antirrhinum majus SAP1 protein. 373 G1944 gi24418033 4.50E−15 Oryzasativa (japonica Hypothetical prote cultivar-group) 373 G1944 gi1002120.0032 Lycopersicon extensin class II (clones esculentum u1/u2) 373G1944 gi167556 0.016 Daucus carota extensin. 373 G1944 gi555655 0.035Nicotiana tabacum DNA-binding protein. 373 G1944 gi72327 0.043 Zea maysglutelin 5-maize. 373 G1944 gi1076237 0.06 Pinus taedaarabinogalactan-like protein- loblolly pine. 373 G1944 gi1247390 0.076Nicotiana alata PRP3. 375 G1946 LPHSF8 1.00E−127 Lycopersicon L.peruvianum Lp-hsf8 peruvianum mRNA for heat 375 G1946 AC087771 4.00E−96Medicago truncatula clone 8D15, *** SEQUENCING IN PROGRESS 375 G1946LEHSF8 3.00E−86 Lycopersicon L. esculentum Le-hsf8 gene esculentum forheat 375 G1946 AW569256 1.00E−84 Glycine max si64g09.y1 Gm-r1030 Glycinemax cDNA clone GENO 375 G1946 AAAA01005302 7.00E−80 Oryza sativa (indica( ) scaffold005302 cultivar-group) 375 G1946 AC120506 1.00E−79 Oryzasativa chromosome 3 clone OSJNBb0006O08, *** SEQUENCI 375 G1946 BG8908992.00E−79 Solanum tuberosum EST516750 cSTD Solanum tuberosum cDNA clo 375G1946 BU834690 8.00E−73 Populus tremula x T064E07 Populus apica Populustremuloides 375 G1946 AV833112 1.00E−60 Hordeum vulgare subsp. AV833112K. Sato vulgare unpublished 375 G1946 BQ916240 4.00E−59 Helianthusannuus QHB17D05.yg.ab1 QH_ABCDI sunflower RHA801 375 G1946 gi1002641.90E−123 Lycopersicon heat shock transcription peruvianum factor H 375G1946 gi100225 9.10E−109 Lycopersicon heat shock transcriptionesculentum factor H 375 G1946 gi24308618 5.40E−63 Oryza sativa (japonicaPutative heat shoc cultivar-group) 375 G1946 gi5821138 5.40E−55Nicotiana tabacum heat shock factor. 375 G1946 gi662924 2.10E−52 Glycinemax heat shock transcription factor 21. 375 G1946 gi25052685 2.50E−51Helianthus annuus heat stress transcription factor HSFA9. 375 G1946gi16118447 4.80E−50 Phaseolus acutifolius heat shock transcriptionfactor. 375 G1946 gi14209551 6.10E−48 Oryza sativa putative heat shockfactor. 375 G1946 gi20162459 1.40E−46 Medicago sativa heat shocktranscription factor. 375 G1946 gi1362193 3.40E−45 Zea mays heat shockfactor-maize. 377 G1947 BE319312 1.00E−49 Medicago truncatulaNF015D08NR1F1035 Nodulated root Medicag 377 G1947 LPHSF30 1.00E−48Lycopersicon L. peruvianum Lp-hsf30 peruvianum mRNA for heat 377 G1947BM086093 5.00E−48 Glycine max sah35d07.y1 Gm-c1036 Glycine max cDNAclone SOY 377 G1947 AV833112 6.00E−47 Hordeum vulgare subsp. AV833112 K.Sato vulgare unpublished 377 G1947 BI406849 2.00E−44 Solanum tuberosum182A06 Mature tuber lambda ZAP Solanum tu 377 G1947 AY099451 2.00E−44Helianthus annuus heat stress transcription factor HSFA9 mR 377 G1947AW034874 8.00E−44 Lycopersicon EST279103 tomato callus, esculentum TAMULycop 377 G1947 AAAA01016817 1.00E−42 Oryza sativa (indica ( )scaffold016817 cultivar-group) 377 G1947 BI305378 6.00E−39 Oryza sativaNRS_2_7_8_A01_K18 Drought stress (root) Oryza 377 G1947 BI4797834.00E−38 Triticum aestivum WHE3452_A08_A16ZS Wheat pre-anthesis spik 377G1947 gi100265 2.10E−47 Lycopersicon heat shock transcription peruvianumfactor H 377 G1947 gi2129828 8.70E−40 Glycine max heat shocktranscription factor HSF21-soybe 377 G1947 gi20521264 4.30E−39 Oryzasativa (japonica putative heat shoc cultivar-group) 377 G1947 gi250526855.50E−38 Helianthus annuus heat stress transcription factor HSFA9. 377G1947 gi14209551 9.30E−38 Oryza sativa putative heat shock factor. 377G1947 gi16118447 1.20E−37 Phaseolus acutifolius heat shock transcriptionfactor. 377 G1947 gi20162459 2.70E−36 Medicago sativa heat shocktranscription factor. 377 G1947 gi5821138 4.50E−36 Nicotiana tabacumheat shock factor. 377 G1947 gi2130133 7.30E−36 Zea mays heat shocktranscription factor (clone hsfa)-m 377 G1947 gi100225 2.50E−35Lycopersicon heat shock transcription esculentum factor H 379 G1948BG321479 1.00E−128 Descurainia sophia Ds01_07g10_ADs01_AAFC_ECORC_cold_stress 379 G1948 BQ704285 1.00E−100 Brassica napusBn01_04d19_A 379 G1948 AC098693 3.00E−92 Oryza sativa chromosome 3 cloneOJ1004_C08, *** SEQUENCING 379 G1948 BH435688 2.00E−88 Brassica oleraceaBOHHK12TF BOHH Brassica oleracea genomic 379 G1948 BI933410 4.00E−59Lycopersicon EST553311 tomato flower, esculentum anthesis L 379 G1948BQ511165 7.00E−58 Solanum tuberosum EST618580 Generation of a set ofpotato c 379 G1948 AAAA01005130 5.00E−57 Oryza sativa (indica ( )scaffold005130 cultivar-group) 379 G1948 BU011081 9.00E−53 Lactucasativa QGJ15D24.yg.ab1 QG_EFGHJ lettuce serriola La 379 G1948 BU0318486.00E−39 Helianthus annuus QHJ19M09.yg.ab1 QH_EFGHJ sunflower RHA280 379G1948 BG300992 3.00E−36 Hordeum vulgare HVSMEb0019C24f Hordeum vulgareseedling sho 379 G1948 gi20502992 5.50E−86 Oryza sativa (japonicaPutative CAO prote cultivar-group) 379 G1948 gi549986 1.40E−12Pennisetum ciliare possible apospory- associated protein. 379 G1948gi19070767 3.10E−12 Oryza sativa apospory-associated protein. 379 G1948gi24637568 5.50E−12 Nicotiana tabacum ankyrin domain protein. 379 G1948gi17645766 9.80E−12 Glycine max unnamed protein product. 379 G1948gi7110220 1.20E−06 Triticum aestivum AKT1-like potassium channel. 379G1948 gi2104908 1.50E−05 Zea mays potassium channel. 379 G1948gi24745936 1.70E−05 Solanum tuberosum ankyrin-like protein. 379 G1948gi20127124 0.00014 Brassica napus calmodulin-binding transcriptionactivator 379 G1948 gi16550932 0.00031 Eucalyptus inward-rectifying K+camaldulensis channel. 381 G1950 BG599002 2.00E−83 Solanum tuberosumEST503902 cSTS Solanum tuberosum cDNA clo 381 G1950 BQ857787 4.00E−78Lactuca sativa QGB8H12.yg.ab1 QG_ABCDI lettuce salinas Lact 381 G1950AW100050 3.00E−69 Glycine max sd25e07.y1 Gm-c1012 Glycine max cDNA cloneGENO 381 G1950 BF177815 4.00E−60 Lotus japonicus Ljirnpest34-724-d7Ljirnp Lambda HybriZap t 381 G1950 BG466155 1.00E−59 Euphorbia esula00918 leafy spurge Lambda HybriZAP 2.1 two- 381 G1950 BU820489 3.00E−55Populus tremula UB10CPG06 Populus tremula cambium cDNA libr 381 G1950BE443704 1.00E−50 Triticum aestivum WHE1121_C05_E09ZS Wheat etiolatedseedlin 381 G1950 BG267984 3.00E−49 Zea mays 1000144D01.x1 1000- UnigeneI from Maize Genome P 381 G1950 BI266915 1.00E−47 Medicago truncatulaNF097B04IN1F1041 Insect herbivory Medic 381 G1950 BM412345 2.00E−46Lycopersicon EST586672 tomato breaker esculentum fruit Lyco 381 G1950gi15341604 1.00E−77 Oryza sativa putative ankyrin. 381 G1950 gi247459365.80E−21 Solanum tuberosum ankyrin-like protein. 381 G1950 gi133108119.00E−17 Nicotiana tabacum ankyrin-repeat protein HBP1. 381 G1950gi7110220 1.10E−16 Triticum aestivum AKT1-like potassium channel. 381G1950 gi21328024 4.00E−16 Oryza sativa (japonica putative AKT1-likecultivar-group) 381 G1950 gi17645764 9.90E−16 Glycine max unnamedprotein product. 381 G1950 gi549986 1.60E−15 Pennisetum ciliare possibleapospory- associated protein. 381 G1950 gi2104908 4.30E−15 Zea mayspotassium channel. 381 G1950 gi2832781 3.70E−13 Egeria densa inwardpotassium channel alpha subunit. 381 G1950 gi8896127 1.20E−11Mesembryanthemum putative potassium channel crystallinum 383 G1958BH495974 5.00E−76 Brassica oleracea BOHHB37TF BOHH Brassica oleraceagenomic 383 G1958 AB017693 7.00E−70 Nicotiana tabacum WERBP-1 mRNA,complete cds. 383 G1958 AF219972 4.00E−62 Mesembryanthemum CDPKsubstrate protein 1 crystallinum (csp 383 G1958 AW507631 1.00E−60Glycine max si42c09.y1 Gm-r1030 Glycine max cDNA clone GENO 383 G1958AW684291 6.00E−59 Medicago truncatula NF015B02NR1F1000 Nodulated rootMedicag 383 G1958 BQ806133 4.00E−58 Triticum aestivum WHE3575_B11_C21ZSWheat developing grains 383 G1958 AW030183 3.00E−56 LycopersiconEST273438 tomato callus, esculentum TAMU Lycop 383 G1958 BQ5877502.00E−53 Beta vulgaris E012340-024-010-G07-SP6 MPIZ-ADIS-024-leaf Be 383G1958 AY107734 2.00E−53 Zea mays PCO065209 mRNA sequence. 383 G1958CA516596 4.00E−51 Capsicum annuum KS09060E12 KS09 Capsicum annuum cDNA,mRNA 383 G1958 gi4519671 3.70E−66 Nicotiana tabacum transfactor. 383G1958 gi6942190 1.50E−58 Mesembryanthemum CDPK substrate protein 1; Ccrystallinum 383 G1958 gi5916207 9.80E−27 Chlamydomonas regulatoryprotein of P- reinhardtii starvat 383 G1958 gi23306130 6.00E−13 Oryzasativa (japonica Unknown protein. cultivar-group) 383 G1958 gi152899818.40E−13 Oryza sativa hypothetical protein. 383 G1958 gi111775406.60E−10 Zea mays putative transcription factor Golden2. 383 G1958gi1946222 0.81 Malus domestica knotted1-like homeobox protein. 383 G1958gi15144509 0.96 Lycopersicon unknown. esculentum 383 G1958 gi23176760.96 Fagopyrum esculentum declined protein during seed develo 383 G1958gi538502 0.96 Stylosanthes humilis peroxidase. 385 G2007 AF1617119.00E−78 Pimpinella brachycarpa myb-related transcription factor mRN 385G2007 CA783329 7.00E−75 Glycine max sat22g04.y1 Gm-c1036 Glycine maxcDNA clone SOY 385 G2007 BU811821 1.00E−69 Populus tremula x UL88TH12Populus leaf Populus tremuloides 385 G2007 AI770808 1.00E−67 Zea mays606058F03.x2 606-Ear tissue cDNA library from Sc 385 G2007 OSA3110532.00E−67 Oryza sativa mRNA for Myb15 protein (myb15 gene). 385 G2007LETHM16 2.00E−66 Lycopersicon L. esculentum mRNA for esculentummyb-related t 385 G2007 BQ624834 5.00E−65 Citrus sinensis USDA-FP_01925Ridge pineapple sweet orange 385 G2007 BU868208 2.00E−64 Populusbalsamifera M112E10 Populus flow subsp. trichocarpa 385 G2007 AW6855867.00E−64 Medicago truncatula NF032A05NR1F1000 Nodulated root Medicag 385G2007 BQ245626 2.00E−62 Triticum aestivum TaE15022B12R TaE15 Triticumaestivum cDNA 385 G2007 gi6651292 7.50E−78 Pimpinella brachycarpamyb-related transcription factor. 385 G2007 gi23343577 4.60E−66 Oryzasativa Myb13 protein. 385 G2007 gi1430846 4.10E−65 Lycopersiconmyb-related transcription esculentum factor. 385 G2007 gi190727405.00E−60 Zea mays typical P-type R2R3 Myb protein. 385 G2007 gi190733304.50E−59 Sorghum bicolor typical P-type R2R3 Myb protein. 385 G2007gi20563 2.60E−53 Petunia x hybrida protein 1. 385 G2007 gi22638 3.00E−53Physcomitrella patens Pp2. 385 G2007 gi13346194 1.50E−51 Gossypiumhirsutum GHMYB9. 385 G2007 gi19386839 2.60E−51 Oryza sativa (japonicaputative myb-relat cultivar-group) 385 G2007 gi4886264 5.90E−50Antirrhinum majus Myb-related transcription factor mixta- 387 G2010BH969114 2.00E−41 Brassica oleracea odg08d11.b1 B. oleracea002 Brassicaolerac 387 G2010 BQ847567 1.00E−34 Lactuca sativa QGA3h03.yg.ab1QG_ABCDI lettuce salinas Lact 387 G2010 BG525285 4.00E−34 Steviarebaudiana 48-3 Stevia field grown leaf cDNA Stevia 387 G2010 BI9282135.00E−34 Lycopersicon EST548102 tomato flower, esculentum 3-8 mm b 387G2010 BU824105 8.00E−34 Populus tremula UB60BPD08 Populus tremulacambium cDNA libr 387 G2010 AMSPB1 1.00E−32 Antirrhinum majus A. majusmRNA for squamosa-promoter bindin 387 G2010 CA516258 3.00E−32 Capsicumannuum KS09055D03 KS09 Capsicum annuum cDNA, mRNA 387 G2010 BE0584325.00E−32 Glycine max sn16a06.y1 Gm-c1016 Glycine max cDNA clone GENO 387G2010 BG455868 6.00E−32 Medicago truncatula NF068F05PL1F1045 Phosphatestarved leaf 387 G2010 BU028945 2.00E−30 Helianthus annuusQHH6J19.yg.ab1 QH_EFGHJ sunflower RHA280 387 G2010 gi1183866 2.50E−33Antirrhinum majus squamosa-promoter binding protein 1. 387 G2010gi5931780 1.10E−27 Zea mays SBP-domain protein 2. 387 G2010 gi84680362.30E−23 Oryza sativa Similar to Arabidopsis thaliana chromosome 2 387G2010 gi9087308 1.50E−10 Mitochondrion Beta orf102a. vulgaris var.altissima 387 G2010 gi22535625 0.53 Oryza sativa (japonica hypotheticalprote cultivar-group) 387 G2010 gi14597634 1 Physcomitrella patens15_ppprot1_080_c02. 387 G2010 gi7209500 1 Brassica rapa S-locus pollenprotein. 389 G2053 BH923697 3.00E−31 Brassica oleracea odi23h12.b1 B.oleracea002 Brassica olerac 389 G2053 AF532619 2.00E−25 Glycine max noapical meristem-like protein mRNA, complete 389 G2053 AF509874 2.00E−24Petunia x hybrida nam-like protein 11 (NH11) mRNA, complete 389 G2053BQ864249 4.00E−24 Lactuca sativa QGC26D03.yg.ab1 QG_ABCDI lettucesalinas Lac 389 G2053 BI246023 8.00E−24 Sorghum bicolorIP1_66_F11.b1_A002 Immature pannicle 1 (IP1 389 G2053 CA815703 1.00E−23Vitis vinifera CA12EI204IVF_E10 Cabernet Sauvignon Leaf-C 389 G2053BQ586991 1.00E−23 Beta vulgaris E012352-024-011-F06-SP6MPIZ-ADIS-024-leaf Be 389 G2053 BF645220 1.00E−23 Medicago truncatulaNF032F12EC1F1102 Elicited cell culture 389 G2053 BU894596 1.00E−23Populus tremula x X011H04 Populus wood Populus tremuloides 389 G2053BG543974 1.00E−23 Brassica rapa subsp. E1725 Chinese cabbage pekinensisetiol 389 G2053 gi22597158 6.50E−28 Glycine max no apical meristem-likeprotein. 389 G2053 gi21105736 2.00E−26 Petunia x hybrida nam-likeprotein 4. 389 G2053 gi15148912 8.50E−26 Phaseolus vulgaris NAC domainprotein NAC1. 389 G2053 gi19225018 1.10E−25 Oryza sativa (japonicaputative NAM (no a cultivar-group) 389 G2053 gi7716952 1.10E−25 Medicagotruncatula NAC1. 389 G2053 gi6175246 2.30E−25 Lycopersicon jasmonic acid2. esculentum 389 G2053 gi4218535 2.00E−24 Triticum sp. GRAB1 protein.389 G2053 gi6730936 2.00E−24 Oryza sativa OsNAC3 protein. 389 G2053gi6732154 2.00E−24 Triticum monococcum unnamed protein product. 389G2053 gi14485513 6.20E−23 Solanum tuberosum putative NAC domain protein.391 G2059 AW257352 3.00E−44 Medicago truncatula EST305489 KV2 Medicagotruncatula cDNA 391 G2059 BI972689 1.00E−36 Glycine max sai81e12.y1Gm-c1065 Glycine max cDNA clone GEN 391 G2059 BQ408107 5.00E−29Gossypium arboreum GA_Ed0006B09f Gossypium arboreum 7-10 d 391 G2059BI922932 2.00E−28 Lycopersicon EST542836 tomato callus esculentumLycopersico 391 G2059 CA018649 6.00E−28 Hordeum vulgare subsp. HV09E02rHV Hordeum vulgare vulgare 391 G2059 BM406373 6.00E−28 Solanum tuberosumEST580796 potato roots Solanum tuberosum 391 G2059 AW618459 1.00E−27Lycopersicon pennellii EST320445 L. pennellii trichome, Cor 391 G2059BI958427 1.00E−27 Hordeum vulgare HVSMEn0014O18f Hordeum vulgare rachisEST 1 391 G2059 BU894329 1.00E−27 Populus tremula x X007E05 Populus woodPopulus tremuloides 391 G2059 AI166481 5.00E−27 Populus balsamiferaxylem.est.309 Poplar subsp. trichocarpa 391 G2059 gi19920190 1.40E−29Oryza sativa (japonica Putative AP2 domai cultivar-group) 391 G2059gi8571476 1.90E−28 Atriplex hortensis apetala2 domain-containingprotein. 391 G2059 gi21908036 4.60E−27 Zea mays DRE binding factor 1.391 G2059 gi14140163 3.30E−23 Oryza sativa putative AP2 domaincontaining protein. 391 G2059 gi131754 1.60E−19 Lupinus polyphyllusPPLZ02 PROTEIN. 391 G2059 gi3342211 2.10E−19 Lycopersicon Pti4.esculentum 391 G2059 gi1208497 1.90E−18 Nicotiana tabacum EREBP-4. 391G2059 gi20303011 5.70E−18 Brassica napus CBF-like protein CBF5. 391G2059 gi19071243 7.40E−18 Hordeum vulgare CRT/DRE binding factor 1. 391G2059 gi7528276 9.70E−18 Mesembryanthemum AP2-related transcription fcrystallinum 393 G2085 BI498544 7.00E−59 Glycine max sai15e07.y1Gm-c1053 Glycine max cDNA clone GEN 393 G2085 BM437375 8.00E−47 Vitisvinifera VVA018E12_54245 An expressed sequence tag da 393 G2085 BI3082047.00E−46 Medicago truncatula EST529614 GPOD Medicago truncatula cDNA 393G2085 BQ295376 8.00E−45 Triticum aestivum WHE2869_C08_F15ZS Wheatunstressed root t 393 G2085 BF199732 7.00E−44 Triticum monococcumWHE0591- 0594_H22_H22ZE Triticum monococ 393 G2085 AY103800 2.00E−43 Zeamays PCO084138 mRNA sequence. 393 G2085 BH723453 3.00E−40 Brassicaoleracea BOMBQ10TR BO_2_3_KB Brassica oleracea gen 393 G2085 BU9930004.00E−39 Hordeum vulgare HD12E09r HD Hordeum vulgare cDNA clone HD12 393G2085 BU815658 4.00E−38 Populus tremula x N044F04 Populus bark Populustremuloides 393 G2085 BQ987329 1.00E−36 Lactuca sativa QGF11O18.yg.ab1QG_EFGHJ lettuce serriola La 393 G2085 gi13174240 3.20E−42 Oryza sativaputative zinc finger protein. 393 G2085 gi23237937 4.30E−09 Oryza sativa(japonica transposase-like. cultivar-group) 393 G2085 gi12711287 0.00061Nicotiana tabacum GATA-1 zinc finger protein. 393 G2085 gi216551620.0027 Hordeum vulgare subsp. CONSTANS-like protein vulgare CO9. 393G2085 gi1076609 0.015 Nicotiana NTL1 protein-curled- plumbaginifolialeaved to 393 G2085 gi22854920 0.017 Brassica nigra COL1 protein. 393G2085 gi3341723 0.082 Raphanus sativus CONSTANS-like 1 protein. 393G2085 gi21667485 0.15 Hordeum vulgare CONSTANS-like protein. 393 G2085gi4091804 0.46 Malus x domestica CONSTANS-like protein 1. 393 G2085gi2303681 0.49 Brassica napus unnamed protein product. 395 G2105BM110736 3.00E−50 Solanum tuberosum EST558272 potato roots Solanumtuberosum 395 G2105 BQ866994 2.00E−49 Lactuca sativa QGC9I02.yg.ab1QG_ABCDI lettuce salinas Lact 395 G2105 BH975294 6.00E−45 Brassicaoleracea odh15d05.b1 B. oleracea002 Brassica olerac 395 G2105 BF6466152.00E−41 Medicago truncatula NF066C08EC1F1065 Elicited cell culture 395G2105 OSGT2 4.00E−32 Oryza sativa O. sativa gt-2 gene. 395 G2105AI777252 1.00E−28 Lycopersicon EST258217 tomato esculentum resistant,Cornell 395 G2105 BU049946 3.00E−27 Zea mays 1111017E09.y1 1111- UnigeneIII from Maize Genome 395 G2105 AB052729 4.00E−26 Pisum sativum mRNA forDNA-binding protein DF1, complete cd 395 G2105 AF372499 4.00E−25 Glycinemax GT-2 factor mRNA, partial cds. 395 G2105 BU889446 4.00E−24 Populustremula P021A05 Populus petioles cDNA library Popul 395 G2105 gi136469862.40E−39 Pisum sativum DNA-binding protein DF1. 395 G2105 gi202492.20E−35 Oryza sativa gt-2. 395 G2105 gi18182311 1.70E−27 Glycine maxGT-2 factor. 395 G2105 gi20161567 9.10E−08 Oryza sativa (japonicahypothetical prote cultivar-group) 395 G2105 gi170271 1.70E−05 Nicotianatabacum DNA-binding protein. 395 G2105 gi4456620 0.36 Hordeum vulgarealpha-galactosidase. 395 G2105 gi3645898 0.68 Zea mays in-frame stopcodon; possibly a post-transpositi 395 G2105 gi531098 0.95 Zinniaelegans TED3. 395 G2105 gi1657853 1 Triticum aestivum cold acclimationprotein WCOR825. 395 G2105 gi20086402 1 Isoetes asiatica LFY homolog.397 G2110 BH472587 4.00E−87 Brassica oleracea BOGPM69TR BOGP Brassicaoleracea genomic 397 G2110 BI422533 9.00E−57 Lycopersicon EST533199tomato callus, esculentum TAMU Lycop 397 G2110 AP002486 1.00E−52 Oryzasativa genomic DNA, chromosome 1, PAC clone: P0510F03. 397 G2110AAAA01001635 2.00E−52 Oryza sativa (indica ( ) scaffold001635cultivar-group) 397 G2110 BM370908 1.00E−47 Hordeum vulgareEBro04_SQ002_M09_R IGF Barley EBro04 librar 397 G2110 AU083645 5.00E−44Cryptomeria japonica AU083645 Cryptomeria japonica inner ba 397 G2110BG551253 2.00E−43 Glycine max sad35a10.y1 Gm-c1074 Glycine max cDNAclone GEN 397 G2110 BQ625082 3.00E−43 Citrus sinensis USDA-FP_02173Ridge pineapple sweet orange 397 G2110 BF636342 5.00E−42 Medicagotruncatula NF088G12DT1F1099 Drought Medicago trunc 397 G2110 BG8387242.00E−40 Glycine clandestina Gc02_02f10_R Gc02_AAFC_ECORC_cold_stres 397G2110 gi11320830 4.00E−59 Oryza sativa putative WRKY DNA bindingprotein. 397 G2110 gi20160973 4.00E−35 Oryza sativa (japonicahypothetical prote cultivar-group) 397 G2110 gi1159879 1.20E−27 Avenafatua DNA-binding protein. 397 G2110 gi11493822 1.50E−27 Petroselinumcrispum transcription factor WRKY4. 397 G2110 gi6683537 1.10E−25Nicotiana tabacum TMV response-related gene product. 397 G2110 gi48949651.70E−20 Avena sativa DNA-binding protein WRKY1. 397 G2110 gi181586197.50E−20 Retama raetam WRKY-like drought- induced protein. 397 G2110gi24745606 2.20E−19 Solanum tuberosum WRKY-type DNA binding protein. 397G2110 gi1076685 3.90E−19 Ipomoea batatas SPF1 protein-sweet potato. 397G2110 gi13620227 4.30E−19 Lycopersicon hypothetical protein. esculentum399 G2114 AX555218 2.00E−99 Glycine max Sequence 3 from PatentWO02059332. 399 G2114 AX555220 2.00E−94 Oryza sativa Sequence 5 fromPatent WO02059332. 399 G2114 AF317904 3.00E−94 Brassica napus AP2/EREBPtranscription factor BABY BOOM1 (B 399 G2114 AY109146 3.00E−89 Zea maysPCO137288 mRNA sequence. 399 G2114 BJ188928 9.00E−87 Physcomitrellapatens BJ188928 normalized ful subsp. patens 399 G2114 BQ864461 2.00E−78Lactuca sativa QGC26M12.yg.ab1 QG_ABCDI lettuce salinas Lac 399 G2114BQ122372 2.00E−74 Medicago truncatula EST607948 GLSD Medicago truncatulacDNA 399 G2114 BQ625052 2.00E−70 Citrus sinensis USDA-FP_02143 Ridgepineapple sweet orange 399 G2114 AJ475492 2.00E−66 Hordeum vulgareAJ475492 S00008 Hordeum vulgare cDNA clone 399 G2114 BJ312281 5.00E−66Triticum aestivum BJ312281 Y. Ogihara unpublished cDNA libr 399 G2114gi21069051 2.10E−95 Brassica napus AP2/EREBP transcription factor BABYBOOM1. 399 G2114 gi21304227 7.10E−90 Oryza sativa ovule developmentaintegumenta-like protein 399 G2114 gi20161013 9.10E−90 Oryza sativa(japonica putative ovule dev cultivar-group) 399 G2114 gi26529381.20E−83 Zea mays orf. 399 G2114 gi18476518 2.10E−45 Hordeum vulgareAPETALA2-like protein. 399 G2114 gi5081557 2.60E−45 Petunia x hybridaPHAP2B protein. 399 G2114 gi11181612 6.40E−44 Picea abiesAPETALA2-related transcription factor 2. 399 G2114 gi13173164 9.40E−43Pisum sativum APETAL2-like protein. 399 G2114 gi21717332 4.10E−42 Malusx domestica transcription factor AHAP2. 399 G2114 gi5360996 1.10E−34Hyacinthus orientalis APETALA2 protein homolog HAP2. 401 G2117 BH9281533.00E−36 Brassica oleracea odi35d09.b1 B. oleracea002 Brassica olerac401 G2117 BU080897 9.00E−21 Glycine max saq31e07.y1 Gm-c1045 Glycine maxcDNA clone SOY 401 G2117 BI977302 9.00E−21 Rosa chinensis eG09 Old Blushpetal SMART library Rosa chin 401 G2117 BI417596 4.00E−19 Lotusjaponicus LjNEST33b4r Lotus japonicus nodule library 401 G2117 BE4508596.00E−19 Lycopersicon EST401746 tomato root, esculentum plants pre-a 401G2117 BE941078 6.00E−19 Medicago truncatula EST420657 MGHG Medicagotruncatula cDNA 401 G2117 BM300051 8.00E−19 MesembryanthemumMCR054F01_24630 Ice crystallinum plant Lam 401 G2117 AF350505 2.00E−17Phaseolus vulgaris bZip transcription factor mRNA, complete 401 G2117AY026054 8.00E−17 Phaseolus acutifolius bZIP mRNA, complete cds. 401G2117 AAAA01000368 1.00E−14 Oryza sativa (indica ( ) scaffold000368cultivar-group) 401 G2117 gi13430400 1.50E−19 Phaseolus vulgaris bZiptranscription factor. 401 G2117 gi12829956 3.20E−19 Phaseolusacutifolius bZIP. 401 G2117 gi10241920 8.00E−14 Nicotiana tabacum TBZF.401 G2117 gi5901747 5.60E−13 Lycopersicon bZIP DNA-binding protein.esculentum 401 G2117 gi9650826 5.60E−13 Petroselinum crispum commonplant regulatory factor 6. 401 G2117 gi22597162 5.10E−12 Glycine maxbZIP transcription factor ATB2. 401 G2117 gi2244742 5.10E−12 Antirrhinummajus bZIP DNA-binding protein. 401 G2117 gi13236840 2.00E−11Catharanthus roseus G-box binding factor bZIP transcripti 401 G2117gi435942 4.40E−11 Oryza sativa DNA-binding factor of bZIP class. 401G2117 gi24460973 1.50E−10 Capsicum chinense bZIP transcription factor.403 G2123 AX281102 2.00E−58 Physcomitrella patens Sequence 8 from PatentWO0177355. 403 G2123 BU836035 1.00E−56 Populus tremula x T081H08 Populusapica Populus tremuloides 403 G2123 AF272573 2.00E−56 Populus alba xPopulus clone INRA717-1-B4 14-3- tremula 3 pr 403 G2123 BM4367312.00E−56 Vitis vinifera VVA008H10_53045 An expressed sequence tag da 403G2123 AB071968 4.00E−55 Nicotiana tabacum D75 mRNA for 14-3-3 protein,complete cds 403 G2123 BM411329 4.00E−55 Lycopersicon EST585656 tomatobreaker esculentum fruit Lyco 403 G2123 BM408090 4.00E−55 Solanumtuberosum EST582417 potato roots Solanum tuberosum 403 G2123 BG5814825.00E−55 Medicago truncatula EST483216 GVN Medicago truncatula cDNA 403G2123 BQ994376 5.00E−55 Lactuca sativa QGF7A23.yg.ab1 QG_EFGHJ lettuceserriola Lac 403 G2123 AF228501 1.00E−54 Glycine max 14-3-3-like proteinmRNA, complete cds. 403 G2123 gi8515890 1.10E−55 Populus alba x Populus14-3-3 protein. tremula 403 G2123 gi8099061 2.30E−55 Populus x canescens14-3-3 protein. 403 G2123 gi15637114 2.10E−54 Lycopersicon 14-3-3 familyprotein. esculentum 403 G2123 gi15778154 2.70E−54 Nicotiana tabacum14-3-3 protein. 403 G2123 gi1575731 5.50E−54 Glycine max SGF14D. 403G2123 gi2822483 3.90E−53 Maackia amurensis 14-3-3 protein homolog. 403G2123 gi6752903 6.30E−53 Euphorbia esula 14-3-3-like protein. 403 G2123gi1076543 1.30E−52 Vicia faba 14-3-3 protein homolog Vfa-1433b-favabean. 403 G2123 gi16755676 1.30E−52 Fritillaria cirrhosa 14-3-3 protein.403 G2123 gi15984178 3.50E−52 Nicotiana benthamiana unnamed proteinproduct. 405 G2130 BH556628 1.00E−83 Brassica oleracea BOHAM82TF BOHABrassica oleracea genomic 405 G2130 AP004902 4.00E−35 Lotus japonicusgenomic DNA, chromosome 2, clone: LjT04G24, 405 G2130 AW685524 5.00E−34Medicago truncatula NF031C12NR1F1000 Nodulated root Medicag 405 G2130BM886518 2.00E−30 Glycine max sam17f08.y1 Gm-c1068 Glycine max cDNAclone SOY 405 G2130 LEU89257 1.00E−28 Lycopersicon DNA-binding proteinPti6 esculentum mRNA, comp 405 G2130 AAAA01000263 3.00E−27 Oryza sativa(indica ( ) scaffold000263 cultivar-group) 405 G2130 AB026295 3.00E−27Oryza sativa genomic DNA, chromosome 6, clone: P0681F10, com 405 G2130BQ873772 6.00E−27 Lactuca sativa QGI2I03.yg.ab1 QG_ABCDI lettuce salinasLact 405 G2130 AF058827 2.00E−25 Nicotiana tabacum TSI1 (Tsi1) mRNA,complete cds. 405 G2130 BQ704534 2.00E−20 Brassica napus Bn01_03k04_A405 G2130 gi2213785 5.50E−31 Lycopersicon Pti6. esculentum 405 G2130gi5295944 1.70E−29 Oryza sativa Similar to Nicotiana tabacum mRNA forERF1, 405 G2130 gi3065895 1.30E−27 Nicotiana tabacum TSI1. 405 G2130gi8809571 6.00E−22 Nicotiana sylvestris ethylene-responsive elementbinding 405 G2130 gi7528276 6.00E−22 Mesembryanthemum AP2-relatedtranscription f crystallinum 405 G2130 gi22415744 1.20E−21 Zea mays AP2domain transcription factor. 405 G2130 gi8571476 1.40E−20 Atriplexhortensis apetala2 domain-containing protein. 405 G2130 gi248172503.50E−20 Cicer arietinum transcription factor EREBP- like protein. 405G2130 gi20805105 6.30E−20 Oryza sativa (japonica contains ESTs AU06cultivar-group) 405 G2130 gi4099921 2.50E−19 Stylosanthes hamata EREBP-3homolog. 407 G2133 BH420519 1.00E−53 Brassica oleracea BOGUH88TF BOGUBrassica oleracea genomic 407 G2133 BG543936 6.00E−43 Brassica rapasubsp. E1686 Chinese cabbage pekinensis etiol 407 G2133 AU2926032.00E−28 Zinnia elegans AU292603 zinnia cultured mesophyll cell equa 407G2133 BE320193 6.00E−24 Medicago truncatula NF024B04RT1F1029 Developingroot Medica 407 G2133 AP003346 3.00E−22 Oryza sativa chromosome 1 cloneP0434C04, *** SEQUENCING IN 407 G2133 AAAA01000718 3.00E−22 Oryza sativa(indica ( ) scaffold000718 cultivar-group) 407 G2133 AC124836 6.00E−22Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 407 G2133BZ403609 2.00E−20 Zea mays OGABN17TM ZM_0.7_1.5_KB Zea mays genomicclone ZMM 407 G2133 BM985484 6.00E−19 Thellungiella halophila 10_C12_TAth Thellungiella halophil 407 G2133 BM403179 3.00E−17 Selaginellalepidophylla SLA012F10_35741 An expressed seque 407 G2133 gi201612396.90E−24 Oryza sativa (japonica hypothetical prote cultivar-group) 407G2133 gi8571476 6.00E−17 Atriplex hortensis apetala2 domain-containingprotein. 407 G2133 gi14140155 7.80E−16 Oryza sativa putative AP2 domaintranscription factor. 407 G2133 gi5616086 7.00E−15 Brassica napusdehydration responsive element binding pro 407 G2133 gi21908034 8.90E−15Zea mays DRE binding factor 2. 407 G2133 gi19071243 6.30E−14 Hordeumvulgare CRT/DRE binding factor 1. 407 G2133 gi18535580 2.10E−13Lycopersicon putative transcriptional esculentum activato 407 G2133gi1208496 3.30E−13 Nicotiana tabacum EREBP-3. 407 G2133 gi89803134.40E−13 Catharanthus roseus AP2-domain DNA-binding protein. 407 G2133gi15488459 2.20E−12 Triticum aestivum AP2-containing protein. 409 G2138BH545016 2.00E−63 Brassica oleracea BOHFD22TR BOHF Brassica oleraceagenomic 409 G2138 BQ704534 7.00E−43 Brassica napus Bn01_03k04_A 409G2138 AP004902 6.00E−28 Lotus japonicus genomic DNA, chromosome 2,clone: LjT04G24, 409 G2138 BM886518 1.00E−25 Glycine max sam17f08.y1Gm-c1068 Glycine max cDNA clone SOY 409 G2138 AW685524 2.00E−25 Medicagotruncatula NF031C12NR1F1000 Nodulated root Medicag 409 G2138 BQ8737721.00E−23 Lactuca sativa QGI2I03.yg.ab1 QG_ABCDI lettuce salinas Lact 409G2138 AF058827 9.00E−22 Nicotiana tabacum TSI1 (Tsi1) mRNA, completecds. 409 G2138 LEU89257 2.00E−20 Lycopersicon DNA-binding protein Pti6esculentum mRNA, comp 409 G2138 BG350434 5.00E−20 Solanum tuberosum091E08 Mature tuber lambda ZAP Solanum tu 409 G2138 AP002835 1.00E−17Oryza sativa genomic DNA, chromosome 1, PAC clone: P0417G05. 409 G2138gi3065895 9.30E−20 Nicotiana tabacum TSI1. 409 G2138 gi5295944 1.50E−19Oryza sativa Similar to Nicotiana tabacum mRNA for ERF1, 409 G2138gi2213785 1.40E−18 Lycopersicon Pti6. esculentum 409 G2138 gi88095731.60E−17 Nicotiana sylvestris ethylene-responsive element binding 409G2138 gi8571476 1.80E−16 Atriplex hortensis apetala2 domain-containingprotein. 409 G2138 gi21908036 4.80E−16 Zea mays DRE binding factor 1.409 G2138 gi3264767 7.80E−16 Prunus armeniaca AP2 domain containingprotein. 409 G2138 gi23617235 1.30E−15 Oryza sativa (japonica containsESTs AU16 cultivar-group) 409 G2138 gi4099921 7.00E−15 Stylosantheshamata EREBP-3 homolog. 409 G2138 gi24817250 1.50E−14 Cicer arietinumtranscription factor EREBP- like protein. 411 G2140 BH501999 1.00E−70Brassica oleracea BOHLI02TF BOHL Brassica oleracea genomic 411 G2140AI488313 5.00E−66 Lycopersicon EST246635 tomato ovary, esculentum TAMULycope 411 G2140 BE020519 2.00E−60 Glycine max sm44g03.y1 Gm-c1028Glycine max cDNA clone GENO 411 G2140 AU093196 1.00E−51 Oryza sativasubsp. AU093196 Rice callus japonica Oryza sat 411 G2140 BF6476872.00E−41 Medicago truncatula NF025A04EC1F1024 Elicited cell culture 411G2140 BH860622 7.00E−39 Populus balsamifera ORNL097 Poplar BAC L subsp.trichocarpa 411 G2140 BU813371 1.00E−38 Populus tremula x N009F04Populus bark Populus tremuloides 411 G2140 AC125495 8.00E−38 Oryzasativa (japonica ( ) chromosome 3 clo cultivar-group) 411 G2140 BU8914904.00E−35 Populus tremula P051C02 Populus petioles cDNA library Popul 411G2140 AI054433 3.00E−34 Mesembryanthemum R6-R97 Ice plant Lambdacrystallinum Uni-Z 411 G2140 gi8570062 8.90E−31 Oryza sativa ESTsC26093(C11622), AU090634 (C12429) corresp 411 G2140 gi21327944 1.80E−30Oryza sativa (japonica contains ESTs AU06 cultivar-group) 411 G2140gi527655 3.80E−10 Pennisetum glaucum myc-like regulatory R gene product.411 G2140 gi527661 7.80E−09 Phyllostachys acuta myc-like regulatory Rgene product. 411 G2140 gi527665 1.70E−08 Sorghum bicolor myc-likeregulatory R gene product. 411 G2140 gi114217 2.60E−08 Zea maysANTHOCYANIN REGULATORY R-S PROTEIN. 411 G2140 gi527663 9.60E−08Tripsacum australe myc-like regulatory R gene product. 411 G2140gi1086526 1.20E−07 Oryza australiensis transcriptional activator Rahomolog. 411 G2140 gi1086534 1.60E−07 Oryza officinalis transcriptionalactivator Ra homolog. 411 G2140 gi1086536 9.20E−07 Oryza rufipogontranscriptional activator Ra homolog. 413 G2143 BH650724 5.00E−76Brassica oleracea BOMIW43TR BO_2_3_KB Brassica oleracea gen 413 G2143CA783614 1.00E−43 Glycine max sat50g04.y1 Gm-c1056 Glycine max cDNAclone SOY 413 G2143 BE451174 9.00E−43 Lycopersicon EST402062 tomatoroot, esculentum plants pre-a 413 G2143 AP004693 6.00E−41 Oryza sativachromosome 8 clone P0461F06, *** SEQUENCING IN 413 G2143 AAAA010068707.00E−40 Oryza sativa (indica ( ) scaffold006870 cultivar-group) 413G2143 AP005655 7.00E−40 Oryza sativa (japonica ( ) chromosome 9 clocultivar-group) 413 G2143 BH775806 2.00E−34 Zea mays fzmb011f018c05f1fzmb filtered library Zea mays ge 413 G2143 AT002234 1.00E−33 Brassicarapa subsp. AT002234 Flower bud pekinensis cDNA Br 413 G2143 BF2634653.00E−27 Hordeum vulgare HV_CEa0006N02f Hordeum vulgare seedling gre 413G2143 CA015528 3.00E−25 Hordeum vulgare subsp. HT14J12r HT Hordeumvulgare vulgare 413 G2143 gi19571105 9.20E−29 Oryza sativa (japonicahypothetical prote cultivar-group) 413 G2143 gi15528743 1.10E−26 Oryzasativa contains EST C74560(E31855)~unknown protein. 413 G2143 gi10865381.60E−09 Oryza rufipogon transcriptional activator Rb homolog. 413 G2143gi6166283 2.30E−09 Pinus taeda helix-loop-helix protein 1A. 413 G2143gi1142621 9.70E−08 Phaseolus vulgaris phaseolin G-box binding proteinPG2. 413 G2143 gi3399777 1.10E−07 Glycine max symbiotic ammoniumtransporter; nodulin. 413 G2143 gi5923912 1.30E−07 Tulipa gesnerianabHLH transcription factor GBOF-1. 413 G2143 gi10998404 1.90E−07 Petuniax hybrida anthocyanin 1. 413 G2143 gi4321762 1.10E−06 Zea maystranscription factor MYC7E. 413 G2143 gi166428 1.30E−06 Antirrhinummajus DEL. 415 G2144 BQ404603 4.00E−59 Gossypium arboreum GA_Ed0072F04fGossypium arboreum 7-10 d 415 G2144 BQ517427 3.00E−53 Solanum tuberosumEST624842 Generation of a set of potato c 415 G2144 BQ583438 3.00E−51Beta vulgaris E011979-024-005-B19-SP6 MPIZ-ADIS-024-inflore 415 G2144BQ122428 6.00E−50 Medicago truncatula EST608004 GLSD Medicago truncatulacDNA 415 G2144 BI427219 1.00E−49 Glycine max sah77g01.y1 Gm-c1049Glycine max cDNA clone GEN 415 G2144 AI725733 1.00E−40 Gossypiumhirsutum BNLGHi12783 Six-day Cotton fiber Gossypi 415 G2144 BH9995512.00E−38 Brassica oleracea oeg96e04.b1 B. oleracea002 Brassica olerac415 G2144 BI926089 1.00E−33 Lycopersicon EST545978 tomato flower,esculentum buds 0-3 m 415 G2144 BU791131 4.00E−33 Populus balsamiferasubsp. trichocarpa x Populus deltoides 415 G2144 BU015022 2.00E−32Lactuca sativa QGJ9A23.yg.ab1 QG_EFGHJ lettuce serriola Lac 415 G2144gi20804997 2.70E−36 Oryza sativa (japonica DNA-binding proteicultivar-group) 415 G2144 gi11862964 2.70E−34 Oryza sativa hypotheticalprotein. 415 G2144 gi5923912 8.60E−33 Tulipa gesneriana bHLHtranscription factor GBOF-1. 415 G2144 gi6166283 5.10E−09 Pinus taedahelix-loop-helix protein 1A. 415 G2144 gi3399777 3.00E−05 Glycine maxsymbiotic ammonium transporter; nodulin. 415 G2144 gi1086538 6.70E−05Oryza rufipogon transcriptional activator Rb homolog. 415 G2144gi13346180 0.00013 Gossypium hirsutum GHDEL61. 415 G2144 gi5276550.00021 Pennisetum glaucum myc-like regulatory R gene product. 415 G2144gi527665 0.00029 Sorghum bicolor myc-like regulatory R gene product. 415G2144 gi527661 0.00033 Phyllostachys acuta myc-like regulatory R geneproduct. 417 G2153 BH566718 1.00E−127 Brassica oleracea BOHCV23TR BOHCBrassica oleracea genomic 417 G2153 AP004971 2.00E−90 Lotus japonicusgenomic DNA, chromosome 5, clone: LjT45G21, 417 G2153 AP004020 1.00E−79Oryza sativa chromosome 2 clone OJ1119_A01, *** SEQUENCING 417 G2153AAAA01017331 2.00E−72 Oryza sativa (indica ( ) scaffold017331cultivar-group) 417 G2153 BQ165495 2.00E−67 Medicago truncatulaEST611364 KVKC Medicago truncatula cDNA 417 G2153 AP005653 1.00E−66Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 417 G2153BQ785950 8.00E−64 Glycine max saq61f09.y1 Gm-c1076 Glycine max cDNAclone SOY 417 G2153 BZ412041 3.00E−63 Zea mays OGACG56TC ZM_0.7_1.5_KBZea mays genomic clone ZMM 417 G2153 BM110212 3.00E−63 Solanum tuberosumEST557748 potato roots Solanum tuberosum 417 G2153 BQ865858 7.00E−63Lactuca sativa QGC6B08.yg.ab1 QG_ABCDI lettuce salinas Lact 417 G2153gi24059979 3.80E−39 Oryza sativa (japonica similar to DNA-bincultivar-group) 417 G2153 gi15528814 1.70E−36 Oryza sativa hypotheticalprotein~similar to Arabidopsis 417 G2153 gi4165183 5.00E−21 Antirrhinummajus SAP1 protein. 417 G2153 gi2213534 1.30E−19 Pisum sativumDNA-binding PD1-like protein. 417 G2153 gi7439981 2.60E−08 Triticumaestivum glycine-rich RNA-binding protein GRP1- 417 G2153 gi216231.90E−06 Sorghum bicolor glycine-rich RNA-binding protein. 417 G2153gi11545668 3.50E−06 Chlamydomonas CIA5. reinhardtii 417 G2153 gi210686726.60E−06 Cicer arietinum putative glicine-rich protein. 417 G2153gi7489714 6.60E−06 Zea mays aluminum-induced protein al1-maize. 417G2153 gi395147 1.60E−05 Nicotiana tabacum glycine-rich protein. 419G2155 BG543096 2.00E−69 Brassica rapa subsp. E0571 Chinese cabbagepekinensis etiol 419 G2155 BH480897 7.00E−66 Brassica oleracea BOGRA01TFBOGR Brassica oleracea genomic 419 G2155 BG646893 2.00E−53 Medicagotruncatula EST508512 HOGA Medicago truncatula cDNA 419 G2155 BU0235703.00E−44 Helianthus annuus QHF11M19.yg.ab1 QH_EFGHJ sunflower RHA280 419G2155 AP004020 2.00E−41 Oryza sativa chromosome 2 clone OJ1119_A01, ***SEQUENCING 419 G2155 BI426899 4.00E−41 Glycine max sag08g12.y1 Gm-c1080Glycine max cDNA clone GEN 419 G2155 AAAA01000383 2.00E−40 Oryza sativa(indica ( ) scaffold000383 cultivar-group) 419 G2155 AP004971 2.00E−40Lotus japonicus genomic DNA, chromosome 5, clone: LjT45G21, 419 G2155AP005755 2.00E−40 Oryza sativa (japonica ( ) chromosome 9 clocultivar-group) 419 G2155 BZ412041 8.00E−39 Zea mays OGACG56TCZM_0.7_1.5_KB Zea mays genomic clone ZMM 419 G2155 gi15528814 3.70E−32Oryza sativa hypothetical protein~similar to Arabidopsis 419 G2155gi24059979 1.20E−21 Oryza sativa (japonica similar to DNA-bincultivar-group) 419 G2155 gi4165183 3.50E−20 Antirrhinum majus SAP1protein. 419 G2155 gi2213534 1.60E−16 Pisum sativum DNA-binding PD1-likeprotein. 419 G2155 gi2224911 0.98 Daucus carota somatic embryogenesisreceptor-like kinase. 419 G2155 gi454279 1 Avena sativa DNA-bindingprotein. 421 G2192 AY061812  1.0e−999 Brassica nigra Lm1 mRNA, completesequence. 421 G2192 BH544406 1.00E−118 Brassica oleracea BOGYW04TF BOGYBrassica oleracea genomic 421 G2192 AC131240 1.00E−98 Medicagotruncatula clone mth2-33j22, WORKING DRAFT SEQUENC 421 G2192 LJA2390413.00E−92 Lotus japonicus mRNA for nodule inception protein (nin). 421G2192 AP001539 2.00E−90 Oryza sativa genomic DNA, chromosome 1, clone:P0708G02. 421 G2192 AAAA01000250 2.00E−90 Oryza sativa (indica ( )scaffold000250 cultivar-group) 421 G2192 BU007504 2.00E−85 Lactucasativa QGH3e07.yg.ab1 QG_EFGHJ lettuce serriola Lac 421 G2192 BF2720612.00E−71 Gossypium arboreum GA_Eb0013L09f Gossypium arboreum 7-10 d 421G2192 BE600221 1.00E−69 Sorghum bicolor PI1_80_G08.b1_A002 Pathogeninduced 1 (PI1) 421 G2192 BG508620 2.00E−66 Glycine max sac75c04.y1Gm-c1072 Glycine max cDNA clone GEN 421 G2192 gi7339715 2.20E−187 Oryzasativa EST AU057816(S21817) corresponds to a region 421 G2192 gi205030012.40E−132 Oryza sativa (japonica Putataive nodule i cultivar-group) 421G2192 gi6448579 3.20E−95 Lotus japonicus nodule inception protein. 421G2192 gi23504757 8.10E−95 Pisum sativum nodule inception protein. 421G2192 gi2190980 0.0002 Chlamydomonas incerta minus dominance geneproduct. 421 G2192 gi1928929 0.0021 Chlamydomonas minus dominanceprotein. reinhardtii 421 G2192 gi100897 0.48 Zea mays Lc regulatoryprotein- maize. 421 G2192 gi170732 0.93 Triticum aestivum gamma-gliadin.421 G2192 gi13346180 0.97 Gossypium hirsutum GHDEL61. 421 G2192 gi1002121 Lycopersicon extensin class II (clones esculentum u1/u2) 423 G2295BZ059285 1.00E−27 Brassica oleracea llf45f10.b1 B. oleracea002 Brassicaolerac 423 G2295 AAAA01000422 7.00E−13 Oryza sativa (indica ( )scaffold000422 cultivar-group) 423 G2295 AP002480 7.00E−13 Oryza sativagenomic DNA, chromosome 1, clone: P0469E05. 423 G2295 AW508033 9.00E−11Glycine max si49c04.y1 Gm-r1030 Glycine max cDNA clone GENO 423 G2295AC135316 3.00E−09 Medicago truncatula clone mth2-2018, WORKING DRAFTSEQUENCE 423 G2295 BE054256 3.00E−07 Gossypium arboreum GA_Ea0026J19fGossypium arboreum 7-10 d 423 G2295 BH023181 8.00E−07 Gossypium hirsutumGH_MBb0004F02r Gossypium hirsutum L. Gos 423 G2295 BZ344426 2.00E−06Sorghum bicolor hp63g11.b1 WGS-SbicolorF (JM107 adapted met 423 G2295AX540653 9.00E−06 Zea mays Sequence 9 from Patent WO0240688. 423 G2295BQ583447 1.00E−05 Beta vulgaris E011979-024-005-D15-SP6MPIZ-ADIS-024-inflore 423 G2295 gi8096379 6.20E−15 Oryza sativa Similarto Arabidopsis thaliana chromosome 5 423 G2295 gi15623935 1.40E−09 Oryzasativa (japonica hypothetical prote cultivar-group) 423 G2295 gi31705029.00E−07 Papaver nudicaule APETALA3 homolog PnAP3-2. 423 G2295 gi65809435.40E−06 Picea abies MADS-box transcription factor. 423 G2295 gi69704118.30E−06 Rosa rugosa MADS-box protein. 423 G2295 gi1049022 8.40E−06Sinapis alba transcription factor SaMADS A. 423 G2295 gi3170512 8.90E−06Peperomia hirta APETALA3 homolog PhAP3. 423 G2295 gi23304676 1.00E−05Brassica oleracea var. MADS-box protein FUL-c. botrytis 423 G2295gi4322475 1.30E−05 Eucalyptus globulus putative MADS box tra subsp.globulus 423 G2295 gi3913005 1.30E−05 Panax ginseng AGAMOUS PROTEIN(GAG2). 425 G2340 BU882839 2.00E−53 Populus balsamifera UM82TH11 Populusflo subsp. trichocarpa 425 G2340 BE054276 3.00E−53 Gossypium arboreumGA_Ea0002O18f Gossypium arboreum 7-10 d 425 G2340 PHMYBPH31 6.00E−53Petunia x hybrida P. hybrida myb.Ph3 gene encoding protein 425 G2340BG269414 2.00E−52 Mesembryanthemum L0-3478T3 Ice plant crystallinumLambda Un 425 G2340 BU892831 2.00E−52 Populus tremula P070A09 Populuspetioles cDNA library Popul 425 G2340 CA516461 2.00E−52 Capsicum annuumKS09058G09 KS09 Capsicum annuum cDNA, mRNA 425 G2340 OSMYB1355 4.00E−52Oryza sativa O. sativa mRNA for myb factor, 1355 bp. 425 G2340 BG5926009.00E−52 Solanum tuberosum EST491278 cSTS Solanum tuberosum cDNA clo 425G2340 BG128147 1.00E−51 Lycopersicon EST473793 tomato esculentumshoot/meristem Lyc 425 G2340 BI542536 2.00E−51 Zea mays 949021A03.y1949- Juvenile leaf and shoot cDNA fr 425 G2340 gi21739235 6.30E−53 Oryzasativa OSJNBa0072F16.14. 425 G2340 gi20563 1.70E−52 Petunia x hybridaprotein 1. 425 G2340 gi13346188 5.70E−52 Gossypium hirsutum GHMYB25. 425G2340 gi485867 8.30E−51 Antirrhinum majus mixta. 425 G2340 gi227950393.60E−50 Populus x canescens putative MYB transcription factor. 425G2340 gi19072748 6.70E−49 Zea mays typical P-type R2R3 Myb protein. 425G2340 gi22266675 4.70E−48 Vitis labrusca x Vitis myb-relatedtranscription vinifera 425 G2340 gi19386839 9.90E−48 Oryza sativa(japonica putative myb-relat cultivar-group) 425 G2340 gi234763139.90E−48 Gossypium raimondii myb-like transcription factor 6. 425 G2340gi6552389 1.20E−47 Nicotiana tabacum myb-related transcription factorLBM4. 427 G2343 LETHM1 1.00E−73 Lycopersicon L. esculentum mRNA foresculentum THM1 protein. 427 G2343 BE611938 1.00E−67 Glycine maxsr01h04.y1 Gm-c1049 Glycine max cDNA clone GENO 427 G2343 BH9666279.00E−64 Brassica oleracea odd90f02.g1 B. oleracea002 Brassica olerac427 G2343 AV421932 1.00E−61 Lotus japonicus AV421932 Lotus japonicusyoung plants (two- 427 G2343 BF484214 1.00E−54 Triticum aestivumWHE2309_F07_K13ZS Wheat pre-anthesis spik 427 G2343 BU998112 5.00E−54Hordeum vulgare subsp. HI10A14r HI Hordeum vulgare vulgare 427 G2343AW672062 6.00E−52 Sorghum bicolor LG1_354_G05.b1_A002 Light Grown 1(LG1) Sor 427 G2343 BI311137 6.00E−52 Medicago truncatula EST5312887GESD Medicago truncatula cDN 427 G2343 BQ634727 4.00E−51 Pinus taedaNXRV072_E09_F NXRV (Nsf Xylem Root wood Vertica 427 G2343 AY1087772.00E−50 Zea mays PCO139596 mRNA sequence. 427 G2343 gi1167486 1.10E−66Lycopersicon transcription factor. esculentum 427 G2343 gi133661811.90E−53 Oryza sativa putative transcription factor. 427 G2343gi22093748 2.20E−50 Oryza sativa (japonica putative myb-relatcultivar-group) 427 G2343 gi13346188 7.60E−46 Gossypium hirsutumGHMYB25. 427 G2343 gi22795039 7.60E−46 Populus x canescens putative MYBtranscription factor. 427 G2343 gi20563 8.60E−45 Petunia x hybridaprotein 1. 427 G2343 gi19059 1.50E−44 Hordeum vulgare MybHv33. 427 G2343gi4886264 1.70E−43 Antirrhinum majus Myb-related transcription factormixta- 427 G2343 gi23476313 2.80E−43 Gossypium raimondii myb-liketranscription factor 6. 427 G2343 gi1732247 1.20E−42 Nicotiana tabacumtranscription factor Myb1. 429 G2346 BQ403570 8.00E−43 Gossypiumarboreum GA_Ed0059F05f Gossypium arboreum 7-10 d 429 G2346 AMA0116228.00E−41 Antirrhinum majus mRNA for squamosa promoter binding 429 G2346BQ594361 1.00E−39 Beta vulgaris S015246-024-024-K10-SP6MPIZ-ADIS-024-develop 429 G2346 BZ040748 4.00E−39 Brassica oleracealka41a03.g1 B. oleracea002 Brassica olerac 429 G2346 AW691786 3.00E−35Medicago truncatula NF044B06ST1F1000 Developing stem Medica 429 G2346BQ874863 1.00E−32 Lactuca sativa QGI6H22.yg.ab1 QG_ABCDI lettuce salinasLact 429 G2346 ZMA011618 7.00E−29 Zea mays mRNA for SBP-domain protein5, partial. 429 G2346 BJ245444 3.00E−27 Triticum aestivum BJ245444 Y.Ogihara unpublished cDNA libr 429 G2346 BE596165 3.00E−27 Sorghumbicolor PI1_50_D04.b1_A002 Pathogen induced 1 (PI1) 429 G2346 BG5937874.00E−27 Solanum tuberosum EST492465 cSTS Solanum tuberosum cDNA clo 429G2346 gi5931641 1.40E−41 Antirrhinum majus squamosa promoter bindingprotein-homol 429 G2346 gi5931786 1.70E−34 Zea mays SBP-domain protein5. 429 G2346 gi8468036 7.60E−23 Oryza sativa Similar to Arabidopsisthaliana chromosome 2 429 G2346 gi9087308 3.90E−09 Mitochondrion Betaorf102a. vulgaris var. altissima 429 G2346 gi17425188 0.34 Triticumaestivum low-molecular-weight glutenin subunit g 429 G2346 gi123462 0.96Hordeum vulgare C-HORDEIN (CLONE PC- 919). 429 G2346 gi225589 0.96Hordeum vulgare var. hordein C. distichum 429 G2346 gi18844948 0.99Oryza sativa (japonica hypothetical prote cultivar-group) 431 G2347BH969114 2.00E−53 Brassica oleracea odg08d11.b1 B. oleracea002 Brassicaolerac 431 G2347 BI931517 6.00E−33 Lycopersicon EST551406 tomato flower,esculentum 8 mm to pr 431 G2347 BQ989469 2.00E−32 Lactuca sativaQGF17M03.yg.ab1 QG_EFGHJ lettuce serriola La 431 G2347 CA516258 3.00E−31Capsicum annuum KS09055D03 KS09 Capsicum annuum cDNA, mRNA 431 G2347BE058432 5.00E−31 Glycine max sn16a06.y1 Gm-c1016 Glycine max cDNA cloneGENO 431 G2347 AMSPB1 7.00E−31 Antirrhinum majus A. majus mRNA forsquamosa-promoter bindin 431 G2347 BI071295 1.00E−30 Populus tremula xC054P79U Populus stra Populus tremuloides 431 G2347 BG525285 8.00E−30Stevia rebaudiana 48-3 Stevia field grown leaf cDNA Stevia 431 G2347BU824105 8.00E−30 Populus tremula UB60BPD08 Populus tremula cambium cDNAlibr 431 G2347 L38193 9.00E−30 Brassica rapa BNAF1025E Mustard flowerbuds Brassica rapa c 431 G2347 gi1183864 5.40E−32 Antirrhinum majussquamosa-promoter binding protein 2. 431 G2347 gi5931786 4.60E−27 Zeamays SBP-domain protein 5. 431 G2347 gi8468036 6.90E−25 Oryza sativaSimilar to Arabidopsis thaliana chromosome 2 431 G2347 gi90873081.40E−09 Mitochondrion Beta orf102a. vulgaris var. altissima 431 G2347gi24414128 0.47 Oryza sativa (japonica hypothetical protecultivar-group) 431 G2347 gi13926087 0.99 Pinus taeda alpha-tubulin. 433G2379 BH573917 7.00E−48 Brassica oleracea BOGNX03TF BOGN Brassicaoleracea genomic 433 G2379 AB072391 4.00E−45 Nicotiana tabacum NtSIP1mRNA for 6b- interacting protein 1, 433 G2379 BG544981 7.00E−43 Brassicarapa subsp. E3094 Chinese cabbage pekinensis etiol 433 G2379 BU5736501.00E−41 Prunus dulcis PA_Ea0004L16f Almond developing seed Prunus 433G2379 CA801229 3.00E−40 Glycine max sau02g07.y2 Gm-c1062 Glycine maxcDNA clone SOY 433 G2379 BI925592 4.00E−39 Lycopersicon EST545481 tomatoflower, esculentum buds 0-3 m 433 G2379 AC113333 5.00E−39 Oryza sativa(japonica ( ) chromosome 5 clo cultivar-group) 433 G2379 AAAA010034848.00E−39 Oryza sativa (indica ( ) scaffold003484 cultivar-group) 433G2379 AP003264 5.00E−38 Oryza sativa chromosome 1 clone P0485G01, ***SEQUENCING IN 433 G2379 BQ590717 3.00E−33 Beta vulgarisE012597-024-018-G24-SP6 MPIZ-ADIS-024-storage 433 G2379 gi181491894.80E−50 Nicotiana tabacum 6b-interacting protein 1. 433 G2379gi21644624 2.50E−43 Oryza sativa (japonica putative 6b-interacultivar-group) 433 G2379 gi12597883 2.30E−21 Oryza sativa hypotheticalprotein. 433 G2379 gi6741989 0.5 Zea mays unnamed protein product. 433G2379 gi12231300 0.77 Lycopersicon ripening regulated protein esculentumDDTFR1 433 G2379 gi2253092 0.79 Spinacia oleracea hypothetical protein.433 G2379 gi3288113 0.84 Beta vulgaris elongation factor 1-beta. 433G2379 gi18419641 0.94 Narcissus putative cysteine proteinase.pseudonarcissus 433 G2379 gi1052956 0.99 Ipomoea nil high mobility groupprotein 2 HMG2. 433 G2379 gi14579399 1 Glycine max unknown. 435 G2430BE214029 2.00E−23 Hordeum vulgare HV_CEb0001P06f Hordeum vulgareseedling gre 435 G2430 BQ858556 8.00E−23 Lactuca sativa QGC10J07.yg.ab1QG_ABCDI lettuce salinas Lac 435 G2430 AU289837 1.00E−22 Zinnia elegansAU289837 zinnia cultured mesophyll cell equa 435 G2430 BM326218 1.00E−22Sorghum bicolor PIC1_72_C05.b1_A002 Pathogen-infected compa 435 G2430AB060130 1.00E−22 Zea mays ZmRR8 mRNA for response regulator 8, completecds. 435 G2430 BG129795 3.00E−21 Lycopersicon EST475441 tomatoesculentum shoot/meristem Lyc 435 G2430 D41804 8.00E−21 Oryza sativaRICS4626A Rice shoot Oryza sativa cDNA, mRNAs 435 G2430 BQ1386998.00E−21 Medicago truncatula NF006C02PH1F1017 Phoma-infected Medicag 435G2430 BU760906 3.00E−19 Glycine max sas60c07.y1 Gm-c1023 Glycine maxcDNA clone SOY 435 G2430 BM407041 1.00E−18 Solanum tuberosum EST581368potato roots Solanum tuberosum 435 G2430 gi14189890 4.70E−34 Zea maysresponse regulator 9. 435 G2430 gi24308616 3.00E−32 Oryza sativa(japonica Putative response cultivar-group) 435 G2430 gi6942190 3.40E−09Mesembryanthemum CDPK substrate protein 1; C crystallinum 435 G2430gi15289981 6.50E−09 Oryza sativa hypothetical protein. 435 G2430gi4519671 2.30E−08 Nicotiana tabacum transfactor. 435 G2430 gi59162078.60E−07 Chlamydomonas regulatory protein of P- reinhardtii starvat 435G2430 gi13173408 2.00E−05 Dianthus caryophyllus response regulatorprotein. 435 G2430 gi15131529 0.0024 Fragaria x ananassa ethylenereceptor. 435 G2430 gi22095684 0.0051 Cucumis sativus Ethylene receptor(CS- ETR1). 435 G2430 gi11357140 0.0065 Cucumis melo var. probableethylene receptor reticulatus 437 G2505 BU879250 5.00E−72 Populusbalsamifera V057G12 Populus flow subsp. trichocarpa 437 G2505 BF6458924.00E−70 Medicago truncatula NF042G10EC1F1083 Elicited cell culture 437G2505 AB028186 4.00E−66 Oryza sativa mRNA for OsNAC7 protein, completecds. 437 G2505 BF098091 4.00E−62 Lycopersicon EST428612 tomato nutrientesculentum deficient 437 G2505 BQ483881 5.00E−62 Triticum aestivumWHE3513_F08_K15ZS Wheat unstressed root c 437 G2505 BE060921 3.00E−61Hordeum vulgare HVSMEg0013N15f Hordeum vulgare pre- anthesis 437 G2505AAAA01001925 9.00E−57 Oryza sativa (indica ( ) scaffold001925cultivar-group) 437 G2505 AI161918 1.00E−56 Populus tremula x A009P50UHybrid aspen Populus tremuloides 437 G2505 CA526032 6.00E−54 Capsicumannuum KS12064G06 KS12 Capsicum annuum cDNA, mRNA 437 G2505 AP0054502.00E−53 Oryza sativa (japonica ( ) chromosome 6 clo cultivar-group) 437G2505 gi11875152 1.40E−66 Oryza sativa putative NAM (no apical meristem)protein. 437 G2505 gi20330750 4.30E−63 Oryza sativa (japonica PutativeNAM-like cultivar-group) 437 G2505 gi1279640 4.70E−48 Petunia x hybridaNAM. 437 G2505 gi22597158 6.10E−48 Glycine max no apical meristem-likeprotein. 437 G2505 gi15148914 4.90E−46 Phaseolus vulgaris NAC domainprotein NAC2. 437 G2505 gi4218537 4.40E−45 Triticum sp. GRAB2 protein.437 G2505 gi6732156 4.40E−45 Triticum monococcum unnamed proteinproduct. 437 G2505 gi6175246 1.00E−43 Lycopersicon jasmonic acid 2.esculentum 437 G2505 gi14485513 1.80E−41 Solanum tuberosum putative NACdomain protein. 437 G2505 gi7716952 6.20E−39 Medicago truncatula NAC1.439 G2509 BH989379 8.00E−66 Brassica oleracea oed22b05.b1 B. oleracea002Brassica olerac 439 G2509 BQ138607 4.00E−41 Medicago truncatulaNF005C01PH1F1004 Phoma-infected Medicag 439 G2509 BQ786702 4.00E−36Glycine max saq72b07.y1 Gm-c1076 Glycine max cDNA clone SOY 439 G2509OSJN00240 7.00E−31 Oryza sativa genomic DNA, chromosome 4, BAC clone:OSJNBa0 439 G2509 AAAA01000832 7.00E−31 Oryza sativa (indica ( )scaffold000832 cultivar-group) 439 G2509 BE419451 2.00E−29 Triticumaestivum WWS012.C2R000101 ITEC WWS Wheat Scutellum 439 G2509 BM0625085.00E−29 Capsicum annuum KS01043F09 KS01 Capsicum annuum cDNA, mRNA 439G2509 AI771755 2.00E−28 Lycopersicon EST252855 tomato ovary, esculentumTAMU Lycope 439 G2509 CA015575 7.00E−28 Hordeum vulgare subsp. HT14L19rHT Hordeum vulgare vulgare 439 G2509 BE642320 2.00E−27 Ceratopterisrichardii Cri2_5_L17_SP6 Ceratopteris Spore Li 439 G2509 gi201608542.10E−29 Oryza sativa (japonica hypothetical prote cultivar-group) 439G2509 gi3264767 8.40E−28 Prunus armeniaca AP2 domain containing protein.439 G2509 gi24817250 1.10E−25 Cicer arietinum transcription factorEREBP- like protein. 439 G2509 gi15217291 7.10E−25 Oryza sativa PutativeAP2 domain containing protein. 439 G2509 gi1208498 1.60E−24 Nicotianatabacum EREBP-2. 439 G2509 gi8809571 1.60E−24 Nicotiana sylvestrisethylene-responsive element binding 439 G2509 gi7528276 3.00E−24Mesembryanthemum AP2-related transcription f crystallinum 439 G2509gi1688233 1.10E−23 Solanum tuberosum DNA binding protein homolog. 439G2509 gi4099921 1.60E−23 Stylosanthes hamata EREBP-3 homolog. 439 G2509gi18496063 2.40E−23 Fagus sylvatica ethylene responsive element bindingprote 441 G2517 CA784851 2.00E−41 Glycine max sat90g04.y1 Gm-c1062Glycine max cDNA clone SOY 441 G2517 BQ799236 3.00E−39 Vitis viniferaEST 1405 Green Grape berries Lambda Zap II L 441 G2517 BU884581 2.00E−36Populus tremula x R012F08 Populus root Populus tremuloides 441 G2517BH479877 5.00E−33 Brassica oleracea BOHNX73TR BOHN Brassica oleraceagenomic 441 G2517 AW034229 2.00E−32 Lycopersicon EST277800 tomatocallus, esculentum TAMU Lycop 441 G2517 AV408330 1.00E−31 Lotusjaponicus AV408330 Lotus japonicus young plants (two- 441 G2517 BG8896902.00E−31 Solanum tuberosum EST515541 cSTD Solanum tuberosum cDNA clo 441G2517 BF645445 6.00E−30 Medicago truncatula NF040F10EC1F1090 Elicitedcell culture 441 G2517 BE445081 6.00E−30 Triticum aestivumWHE1131_B06_D11ZS Wheat etiolated seedlin 441 G2517 BE362650 5.00E−28Sorghum bicolor DG1_88_H02.b1_A002 Dark Grown 1 (DG1) Sorgh 441 G2517gi11761085 1.00E−36 Oryza sativa putative DNA-binding protein homolog.441 G2517 gi22830985 7.00E−31 Oryza sativa (japonica WRKY transcriptioncultivar-group) 441 G2517 gi4760692 9.80E−25 Nicotiana tabacumtranscription factor NtWRKY2. 441 G2517 gi18158619 1.50E−23 Retamaraetam WRKY-like drought- induced protein. 441 G2517 gi13620227 2.20E−23Lycopersicon hypothetical protein. esculentum 441 G2517 gi247456063.80E−23 Solanum tuberosum WRKY-type DNA binding protein. 441 G2517gi7484759 1.40E−22 Cucumis sativus SP8 binding protein homolog-cucumber.441 G2517 gi1159877 1.60E−22 Avena fatua DNA-binding protein. 441 G2517gi1076685 6.00E−22 Ipomoea batatas SPF1 protein-sweet potato. 441 G2517gi11493822 1.50E−21 Petroselinum crispum transcription factor WRKY4. 443G2520 AW928317 2.00E−48 Lycopersicon EST307050 tomato flower esculentumbuds 8 mm t 443 G2520 BI270049 3.00E−47 Medicago truncatulaNF004D04FL1F1042 Developing flower Medi 443 G2520 BU832739 8.00E−46Populus tremula x T037F09 Populus apica Populus tremuloides 443 G2520BU009829 2.00E−45 Lactuca sativa QGJ11L06.yg.ab1 QG_EFGHJ lettuceserriola La 443 G2520 BF271147 6.00E−43 Gossypium arboreum GA_Eb0010K15fGossypium arboreum 7-10 d 443 G2520 BG725974 4.00E−42 Glycine maxsae11d10.y1 Gm-c1067 Glycine max cDNA clone GEN 443 G2520 BQ5099302.00E−41 Solanum tuberosum EST617345 Generation of a set of potato c 443G2520 CA522636 6.00E−41 Capsicum annuum KS12008F12 KS12 Capsicum annuumcDNA, mRNA 443 G2520 BH248832 5.00E−40 Brassica oleracea BOGAN13TR BOGABrassica oleracea genomic 443 G2520 BQ105890 1.00E−39 Rosa hybridcultivar fc1141.e Rose Petals (Fragrant Cloud) 443 G2520 gi208049975.10E−35 Oryza sativa (japonica DNA-binding protei cultivar-group) 443G2520 gi11862964 2.10E−34 Oryza sativa hypothetical protein. 443 G2520gi5923912 6.10E−32 Tulipa gesneriana bHLH transcription factor GBOF-1.443 G2520 gi6166283 3.30E−10 Pinus taeda helix-loop-helix protein 1A.443 G2520 gi527655 1.10E−07 Pennisetum glaucum myc-like regulatory Rgene product. 443 G2520 gi527665 4.00E−07 Sorghum bicolor myc-likeregulatory R gene product. 443 G2520 gi527661 1.10E−06 Phyllostachysacuta myc-like regulatory R gene product. 443 G2520 gi13346180 1.90E−06Gossypium hirsutum GHDEL61. 443 G2520 gi3399777 2.60E−06 Glycine maxsymbiotic ammonium transporter; nodulin. 443 G2520 gi1086534 4.90E−06Oryza officinalis transcriptional activator Ra homolog. 445 G2555BF096555 4.00E−42 Lycopersicon EST360582 tomato nutrient esculentumdeficient 445 G2555 BH509718 2.00E−40 Brassica oleracea BOHGV18TF BOHGBrassica oleracea genomic 445 G2555 BF005956 3.00E−40 Medicagotruncatula EST434454 DSLC Medicago truncatula cDNA 445 G2555 BU0915503.00E−35 Glycine max st74e07.y1 Gm-c1054 Glycine max cDNA clone GENO 445G2555 AF465824 1.00E−30 Oryza sativa transcription factor RAU1 (rau1)mRNA, partial 445 G2555 BU499331 2.00E−30 Zea mays 946174A05.y1946-tassel primordium prepared by S 445 G2555 BU866761 6.00E−30 Populustremula x S070E02 Populus imbib Populus tremuloides 445 G2555 CA0141362.00E−29 Hordeum vulgare subsp. HT10H19r HT Hordeum vulgare vulgare 445G2555 BM063750 5.00E−29 Capsicum annuum KS01059B06 KS01 Capsicum annuumcDNA, mRNA 445 G2555 AW160239 6.00E−29 Lycopersicon pennellii EST290097L. pennellii trichome, Cor 445 G2555 gi6166283 1.70E−40 Pinus taedahelix-loop-helix protein 1A. 445 G2555 gi19401700 1.70E−34 Oryza sativatranscription factor RAU1. 445 G2555 gi20161021 2.40E−33 Oryza sativa(japonica contains ESTs AU05 cultivar-group) 445 G2555 gi59239121.70E−11 Tulipa gesneriana bHLH transcription factor GBOF-1. 445 G2555gi1086538 4.50E−06 Oryza rufipogon transcriptional activator Rb homolog.445 G2555 gi3399777 3.90E−05 Glycine max symbiotic ammonium transporter;nodulin. 445 G2555 gi527657 6.20E−05 Pennisetum glaucum myc-likeregulatory R gene product. 445 G2555 gi1142619 0.00059 Phaseolusvulgaris phaseolin G-box binding protein PG1. 445 G2555 gi42061180.00091 Mesembryanthemum transporter homolog. crystallinum 445 G2555gi13346182 0.0027 Gossypium hirsutum GHDEL65. 447 G2557 BH5118401.00E−66 Brassica oleracea BOGRJ19TR BOGR Brassica oleracea genomic 447G2557 CA799720 5.00E−49 Glycine max sat61g07.y1 Gm-c1056 Glycine maxcDNA clone SOY 447 G2557 AP003296 1.00E−35 Oryza sativa chromosome 1clone P0697C12, *** SEQUENCING IN 447 G2557 AAAA01007476 1.00E−33 Oryzasativa (indica ( ) scaffold007476 cultivar-group) 447 G2557 BF2634651.00E−32 Hordeum vulgare HV_CEa0006N02f Hordeum vulgare seedling gre 447G2557 AT002234 3.00E−28 Brassica rapa subsp. AT002234 Flower budpekinensis cDNA Br 447 G2557 AP006057 1.00E−27 Oryza sativa (japonica () chromosome 9 clo cultivar-group) 447 G2557 CA015528 1.00E−27 Hordeumvulgare subsp. HT14J12r HT Hordeum vulgare vulgare 447 G2557 BG5570112.00E−27 Sorghum bicolor EM1_41_E02.g1_A002 Embryo 1 (EM1) Sorghum b 447G2557 BH775806 7.00E−27 Zea mays fzmb011f018c05f1 fzmb filtered libraryZea mays ge 447 G2557 gi15289790 5.00E−37 Oryza sativa contains ESTC74560(E31855)~unknown protein. 447 G2557 gi19571105 8.40E−35 Oryzasativa (japonica hypothetical prote cultivar-group) 447 G2557 gi33997774.60E−07 Glycine max symbiotic ammonium transporter; nodulin. 447 G2557gi4206118 2.10E−06 Mesembryanthemum transporter homolog. crystallinum447 G2557 gi6166283 3.10E−06 Pinus taeda helix-loop-helix protein 1A.447 G2557 gi5923912 6.80E−06 Tulipa gesneriana bHLH transcription factorGBOF-1. 447 G2557 gi527655 6.90E−06 Pennisetum glaucum myc-likeregulatory R gene product. 447 G2557 gi527661 1.50E−05 Phyllostachysacuta myc-like regulatory R gene product. 447 G2557 gi527665 1.80E−05Sorghum bicolor myc-like regulatory R gene product. 447 G2557 gi10865381.90E−05 Oryza rufipogon transcriptional activator Rb homolog. 449 G2583BH658452 1.00E−59 Brassica oleracea BOMCP74TF BO_2_3_KB Brassicaoleracea gen 449 G2583 BE023297 5.00E−54 Glycine max sm80e10.y1 Gm-c1015Glycine max cDNA clone GENO 449 G2583 CA486875 1.00E−50 Triticumaestivum WHE4337_A02_A03ZS Wheat meiotic anther cD 449 G2583 BG6425548.00E−48 Lycopersicon EST356031 tomato flower esculentum buds, anthe 449G2583 BI978981 2.00E−47 Rosa chinensis zD09 Old Blush petal SMARTlibrary Rosa chin 449 G2583 BU978490 4.00E−47 Hordeum vulgare subsp.HA13G05r HA Hordeum vulgare vulgare 449 G2583 BQ106328 4.00E−46 Rosahybrid cultivar gg1388.e Rose Petals (Golden Gate) Lam 449 G2583BI958226 1.00E−44 Hordeum vulgare HVSMEn0013P17f Hordeum vulgare rachisEST 1 449 G2583 AP004869 1.00E−43 Oryza sativa (japonica ( ) chromosome2 clo cultivar-group) 449 G2583 BU832200 6.00E−43 Populus tremula xT030G01 Populus apica Populus tremuloides 449 G2583 gi18650662 2.30E−23Lycopersicon ethylene response factor 1. esculentum 449 G2583 gi1317547.30E−20 Lupinus polyphyllus PPLZ02 PROTEIN. 449 G2583 gi201608542.80E−18 Oryza sativa (japonica hypothetical prote cultivar-group) 449G2583 gi10798644 2.80E−18 Nicotiana tabacum AP2 domain-containingtranscription fac 449 G2583 gi8571476 2.80E−18 Atriplex hortensisapetala2 domain-containing protein. 449 G2583 gi14018047 3.30E−17 Oryzasativa Putative protein containing AP2 DNA binding 449 G2583 gi122258841.10E−16 Zea mays unnamed protein product. 449 G2583 gi3264767 1.10E−16Prunus armeniaca AP2 domain containing protein. 449 G2583 gi40999141.10E−16 Stylosanthes hamata ethylene-responsive element binding p 449G2583 gi8809573 1.40E−16 Nicotiana sylvestris ethylene-responsiveelement binding 451 G2701 AW164275 3.00E−68 Glycine max se70d01.y1Gm-c1023 Glycine max cDNA clone GENO 451 G2701 AF239956 2.00E−58 Heveabrasiliensis unknown mRNA. 451 G2701 BQ115848 3.00E−57 Solanum tuberosumEST601424 mixed potato tissues Solanum tu 451 G2701 AW220831 8.00E−53Lycopersicon EST297300 tomato fruit esculentum mature green 451 G2701BQ992139 4.00E−52 Lactuca sativa QGF24M24.yg.ab1 QG_EFGHJ lettuceserriola La 451 G2701 BE319813 4.00E−48 Medicago truncatulaNF022C09RT1F1066 Developing root Medica 451 G2701 AAAA01017329 2.00E−46Oryza sativa (indica ( ) scaffold017329 cultivar-group) 451 G2701AC130612 2.00E−46 Oryza sativa (japonica ( ) chromosome 5 clocultivar-group) 451 G2701 AP003279 3.00E−45 Oryza sativa chromosome 1clone P0529E05, *** SEQUENCING IN 451 G2701 BG525326 1.00E−42 Steviarebaudiana 48-70 Stevia field grown leaf cDNA Stevia 451 G2701gi12005328 4.20E−56 Hevea brasiliensis unknown. 451 G2701 gi188742633.00E−55 Antirrhinum majus MYB-like transcription factor DIVARICAT 451G2701 gi18461206 1.10E−48 Oryza sativa (japonica contains ESTs AU10cultivar-group) 451 G2701 gi10798825 2.00E−45 Oryza sativa putativemyb-related transcription activator 451 G2701 gi6688529 5.60E−45Lycopersicon I-box binding factor. esculentum 451 G2701 gi199115794.00E−44 Glycine max syringolide-induced protein 1-3-1B. 451 G2701gi15209176 9.20E−43 Solanum demissum putative I-box binding factor. 451G2701 gi12406995 1.20E−26 Hordeum vulgare MCB2 protein. 451 G2701gi7705206 7.60E−25 Solanum tuberosum MybSt1. 451 G2701 gi200676617.00E−15 Zea mays one repeat myb transcriptional factor. 453 G2719BF097761 8.00E−50 Lycopersicon EST415834 tomato nutrient esculentumdeficient 453 G2719 BQ995199 1.00E−49 Lactuca sativa QGF9F12.yg.ab1QG_EFGHJ lettuce serriola Lac 453 G2719 CA785073 6.00E−48 Glycine maxsat27b04.y1 Gm-c1056 Glycine max cDNA clone SOY 453 G2719 AW6893911.00E−47 Medicago truncatula NF018F11ST1F1000 Developing stem Medica 453G2719 BU025163 1.00E−45 Helianthus annuus QHF7P05.yg.ab1 QH_EFGHJsunflower RHA280 453 G2719 AP004467 2.00E−43 Lotus japonicus genomicDNA, chromosome 1, clone: LjT06K11, 453 G2719 BH444284 6.00E−43 Brassicaoleracea BOGON79TF BOGO Brassica oleracea genomic 453 G2719 AAAA010317782.00E−41 Oryza sativa (indica ( ) scaffold031778 cultivar-group) 453G2719 BU875887 4.00E−41 Populus balsamifera V012F11 Populus flow subsp.trichocarpa 453 G2719 AP005821 9.00E−41 Oryza sativa (japonica ( )chromosome 9 clo cultivar-group) 453 G2719 gi20160571 2.90E−64 Oryzasativa (japonica putative MYB trans cultivar-group) 453 G2719 gi99541126.70E−43 Solanum tuberosum tuber-specific and sucrose- responsive e 453G2719 gi6539552 1.60E−33 Oryza sativa Similar to putative transcriptionfactor (AF 453 G2719 gi7677136 7.80E−32 Adiantum raddianum c-myb-liketranscription factor. 453 G2719 gi16326135 1.20E−31 Nicotiana tabacumMyb. 453 G2719 gi7230673 1.60E−31 Papaver rhoeas putative Myb-relateddomain. 453 G2719 gi1200239 8.50E−31 Hordeum vulgare GAMyb protein. 453G2719 gi8745321 8.50E−31 Physcomitrella patens putative c-myb-liketranscription f 453 G2719 gi20565 3.80E−30 Petunia x hybrida protein 3.453 G2719 gi4581969 2.00E−29 Avena sativa myb protein. 455 G2789BH975957 1.00E−77 Brassica oleracea odh67e11.g1 B. oleracea002 Brassicaolerac 455 G2789 AJ502190 4.00E−76 Medicago truncatula AJ502190 MTAMPMedicago truncatula cDNA 455 G2789 AP005653 7.00E−68 Oryza sativa(japonica ( ) chromosome 2 clo cultivar-group) 455 G2789 AAAA010094277.00E−68 Oryza sativa (indica ( ) scaffold009427 cultivar-group) 455G2789 BQ863249 1.00E−65 Lactuca sativa QGC23G02.yg.ab1 QG_ABCDI lettucesalinas Lac 455 G2789 AP003526 6.00E−64 Oryza sativa chromosome 6 cloneP0548D03, *** SEQUENCING IN 455 G2789 BM110212 4.00E−62 Solanumtuberosum EST557748 potato roots Solanum tuberosum 455 G2789 BZ4120412.00E−59 Zea mays OGACG56TC ZM_0.7_1.5_KB Zea mays genomic clone ZMM 455G2789 BG134451 5.00E−59 Lycopersicon EST467343 tomato crown esculentumgall Lycoper 455 G2789 AP004971 4.00E−57 Lotus japonicus genomic DNA,chromosome 5, clone: LjT45G21, 455 G2789 gi15528814 5.70E−36 Oryzasativa hypothetical protein~similar to Arabidopsis 455 G2789 gi240599795.50E−31 Oryza sativa (japonica similar to DNA-bin cultivar-group) 455G2789 gi4165183 4.50E−20 Antirrhinum majus SAP1 protein. 455 G2789gi2213534 8.60E−19 Pisum sativum DNA-binding PD1-like protein. 455 G2789gi14916565 0.98 Malus x domestica Flavonol synthase (FLS). 455 G2789gi1313924 0.98 Brassica oleracea non intermediate filament IFA binding p455 G2789 gi7671199 1 Chlamydomonas flagellar autotomy proteinreinhardtii Fa1p 455 G2789 gi11466352 1 Mesostigma viride photosystem IIprotein N. 457 G2830 BH993354 7.00E−65 Brassica oleracea oeg99c11.g1 B.oleracea002 Brassica olerac 457 G2830 BM177052 3.00E−13 Glycine maxsaj76c01.y1 Gm-c1074 Glycine max cDNA clone SOY 457 G2830 BI137362 5.7Populus balsamifera F084P95Y Populus flo subsp. trichocarpa 457 G2830AC125368 5.7 Medicago truncatula clone mth2-13h15, WORKING DRAFT SEQUENC457 G2830 BG269090 5.7 Mesembryanthemum L0-3090T3 Ice plant crystallinumLambda Un 457 G2830 BE345092 7.5 Zea mays 946031F09.y1 946-tasselprimordium prepared by S 457 G2830 gi8099397 0.97 Nicotiana tabacumprotoporphyrinogen oxidase precursor; p

Table 9 lists sequences discovered to be paralogous to a number oftranscription factors of the present invention. The columns headingsinclude, from left to right, the Arabidopsis SEQ ID NO; correspondingArabidopsis Gene ID (GID) numbers; the GID numbers of the paralogsdiscovered in a database search; and the SEQ ID NOs of the paralogs.

TABLE 9 Arabidopsis Transcription Factors and Paralogs SEQ ID NO: GIDNO. Paralog SEQ ID NO: Paralog GID No. 8 G24 1952, 2090, 2104 G12,G1277, G1379 10 G28 2074 G1006 12 G47 408 G2133 16 G157 166, 350, 352G859, G1842, G1843 20 G175 174 G877 32 G196 1962 G182 36 G214 146 G68038 G226 148 G682 40 G241 1978 G233 44 G254 1974 G228 46 G256 2048, 2050,2066 G666, G668, G932 48 G278 2092 G1290 50 G291 2088 G1211 56 G325 2160G1998 58 G343 1986 G342 60 G353 62 G354 62 G354 60 G353 64 G361 66 G36266 G362 64 G361 70 G390 72, 78 G391, G438 72 G391 70, 78 G390, G438 76G427 1996, 1998, 2188 G425, G426, G2545 78 G438 70, 72 G390, G391 80G450 2002, 2004, 2006 G448, G455, G456 82 G464 2008 G463 88 G481 90,2010 G482, G485, G2345 90 G482 88, 2010 G481, G485 92 G484 2190 G2631 94G489 2054 G714 98 G504 2108, 2110 G1425, G1454 102 G519 2012, 2014, 2060G501, G502, G767 104 G545 1988, 1990 G350, G351 114 G568 2034 G580 116G584 2082 G1136 118 G585 2036 G586 122 G594 294 G1496 136 G652 2096G1335 138 G663 2094, 2174, 2176 G1329, G2421, G2422 140 G664 1964, 1984G197, G255 144 G676 1966, 1980 G212, G247 146 G680 36 G214 148 G682 38,1972, 2142, 2192 G225, G226, G1816, G2718 150 G715 314 G1646 154 G7362182 G2432 160 G789 292 G1494 164 G849 2042 G610 166 G859 16, 350, 352,2130, G157, G1842, 2146 G1843, G1759, G1844 170 G867 1950, 370 G9, G1930174 G877 20 G175 176 G881 2068 G986 180 G896 2098 G1349 186 G912 1958,1960, 1962 G40, G41, G42 188 G913 2162 G2107 194 G961 2186 G2535 198G974 1948 G5 200 G975 450 G2583 202 G979 2164 G2131 204 G987 2202 G3010208 G1040 2056, 2058 G729, G730 210 G1047 2140 G1808 212 G1051 214 G1052214 G1052 212 G1051 216 G1062 2128 G1664 218 G1063 414 G2143 224 G10732078, 2166 G1067, G2156 226 G1075 2080 G1076 232 G1134 446 G2555 234G1140 2064 G861 238 G1146 2084, 2086 G1149, G1152 240 G1196 2062 G839242 G1198 2024, 2026, 2028, G554, G555, G556, 2030, 2032, G558, G578,G629, 2044, 2138 G1806 250 G1255 2122 G1484 258 G1322 1970, 1982 G221,G249 260 G1323 2046 G659 262 G1330 2178 G2423 268 G1363 2132 G1782 270G1411 440 G2509 278 G1451 2070 G990 280 G1452 2016, 2100 G512, G1357 282G1463 2114, 2116, 2118, 2120 G1461, G1462, G1464, G1465 286 G1478 2152G1929 288 G1482 2148 G1888 292 G1494 160 G789 294 G1496 122 G594 306G1634 452 G2701 312 G1645 2180 G2424 316 G1652 2194 G2776 322 G1749 2144G1839 324 G1750 2000 G440 332 G1792 1954, 2134, 2136 G30, G1791, G1795340 G1818 344 G1836 344 G1836 340 G1818 350 G1842 16, 166, 352 G157,G859, G1843 352 G1843 16, 166, 350 G157, G859, G1842 356 G1863 2170G2334 360 G1895 364 G1903 364 G1903 360 G1895 368 G1927 2168 G2184 370G1930 170 G867 374 G1944 2040 G605 386 G2007 1976 G231 388 G2010 432G2347 390 G2053 2018, 2020, 2022 G515, G516, G517 406 G2130 2076 G1008408 G2133 12 G47 414 G2143 218 G1063 420 G2155 2154 G1945 426 G2340 2052G671 432 G2347 388 G2010 440 G2509 270 G1411 446 G2555 232 G1134 450G2583 200 G975 452 G2701 306 G1634 454 G2719 1968 G216 456 G2789 2038G596 1948 G5 198 G974 1950 G9 170, 370 G867, G1930 1952 G12 8 G24 1954G30 332 G1792 1956 G40 1958, 1960, 186 G41, G42, G912 1958 G41 1956,1960, 186 G40, G42, G912 1960 G42 1956, 1958, 186 G40, G41, G912 1962G182 32 G196 1964 G197 140 G664 1966 G212 144 G676 1968 G216 454 G27191970 G221 258 G1322 1972 G225 38, 148 G226, G682 1974 G228 44 G254 1976G231 386 G2007 1978 G233 40 G241 1980 G247 144 G676 1982 G249 258 G13221984 G255 140 G664 1986 G342 58 G343 1988 G350 104 G545 1990 G351 104G545 1992 G370 64, 66 G361, G362 1994 G392 70, 72, 78 G390, G391, G4381996 G425 76 G427 1998 G426 76 G427 2000 G440 324 G1750 2002 G448 80G450 2004 G455 80 G450 2006 G456 80 G450 2008 G463 82 G464 2010 G485 88,90 G481, G482 2012 G501 102 G519 2014 G502 102 G519 2016 G512 280 G14522018 G515 390 G2053 2020 G516 390 G2053 2022 G517 390 G2053 2024 G554242 G1198 2026 G555 242 G1198 2028 G556 242 G1198 2030 G558 242 G11982032 G578 242 G1198 2034 G580 114 G568 2036 G586 118 G585 2038 G596 456G2789 2040 G605 374 G1944 2042 G610 164 G849 2044 G629 242 G1198 2046G659 260 G1323 2048 G666 46 G256 2050 G668 46 G256 2052 G671 426 G23402054 G714 94 G489 2056 G729 208 G1040 2058 G730 208 G1040 2060 G767 102G519 2062 G839 240 G1196 2064 G861 234 G1140 2066 G932 46 G256 2068 G986176 G881 2070 G990 278 G1451 2072 G993 170, 370 G867, G1930 2074 G100610 G28 2076 G1008 406 G2130 2078 G1067 224 G1073 2080 G1076 226 G10752082 G1136 116 G584 2084 G1149 238 G1146 2086 G1152 238 G1146 2088 G121150 G291 2090 G1277 8 G24 2092 G1290 48 G278 2094 G1329 138 G663 2096G1335 136 G652 2098 G1349 180 G896 2100 G1357 280 G1452 2102 G1364 88,90 G481, G482 2104 G1379 8 G24 2106 G1387 200, 450 G975, G2583 2108G1425 98 G504 2110 G1454 98 G504 2114 G1461 282 G1463 2116 G1462 282G1463 2118 G1464 282 G1463 2120 G1465 282 G1463 2122 G1484 250 G12552124 G1548 70, 72, 78 G390, G391, G438 2126 G1646 150 G715 2128 G1664216 G1062 2130 G1759 16, 172, 350, 352 G157, G859, G1842, G1843 2132G1782 268 G1363 2134 G1791 332 G1792 2136 G1795 332 G1792 2138 G1806 242G1198 2140 G1808 210 G1047 2142 G1816 38, 148 G226, G682 2144 G1839 322G1749 2146 G1844 16, 166, 350, 352 G157, G859, G1842, G1843 2148 G1888288 G1482 2150 G1889 60, 62 G353, G354 2152 G1929 286 G1478 2154 G1945420 G2155 2156 G1974 60, 62 G353, G354 2158 G1995 64, 66 G361, G362 2160G1998 56 G325 2162 G2107 186, 188 G912, G913 2164 G2131 202 G979 2166G2156 224 G1073 2168 G2184 368 G1927 2170 G2334 356 G1863 2172 G2345 88,90 G481, G482 2174 G2421 138 G663 2176 G2422 138 G663 2178 G2423 262G1330 2180 G2424 312 G1645 2182 G2432 154 G736 2184 G2513 1956, 1958,1960, 186 G40, G41, G42, G912 2186 G2535 194 G961 2188 G2545 76 G4272190 G2631 92 G484 2192 G2718 38, 148 G226, G682 2194 G2776 316 G16522196 G2826 64, 66 G361, G362, G1995 2198 G2838 64, 66 G361, G362, G19952200 G2839 60, 62 G353, G354 2202 G3010 204 G987

Table 10 lists the gene identification number (GID) and homologousrelationships found using analyses according to Example IX for thesequences of the Sequence Listing.

TABLE 10 Homologous relationships found within the Sequence Listing DNAor Species from Which Protein Homologous Sequence SEQ ID NO: GID No.(PRT) is Derived Relationship of SEQ ID NO: to Other Genes 459 DNAGlycine max Predicted polypeptide sequence is orthologous to G8 460 DNAGlycine max Predicted polypeptide sequence is orthologous to G8 461 DNAGlycine max Predicted polypeptide sequence is orthologous to G8 462 DNAGlycine max Predicted polypeptide sequence is orthologous to G8 463 DNAOryza sativa Predicted polypeptide sequence is orthologous to G8 464 DNAZea mays Predicted polypeptide sequence is orthologous to G8 465 DNA Zeamays Predicted polypeptide sequence is orthologous to G8 466 DNA Zeamays Predicted polypeptide sequence is orthologous to G8 467 PRT Oryzasativa Orthologous to G8 468 DNA Glycine max Predicted polypeptidesequence is orthologous to G19 469 DNA Glycine max Predicted polypeptidesequence is orthologous to G19 470 DNA Glycine max Predicted polypeptidesequence is orthologous to G19 471 DNA Glycine max Predicted polypeptidesequence is orthologous to G19 472 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G19 473 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G19 474 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G19 475 DNA Zeamays Predicted polypeptide sequence is orthologous to G19 476 DNA Zeamays Predicted polypeptide sequence is orthologous to G19 477 DNAGlycine max Predicted polypeptide sequence is orthologous to G22 478 DNAGlycine max Predicted polypeptide sequence is orthologous to G22 479 DNAGlycine max Predicted polypeptide sequence is orthologous to G24 480 DNAGlycine max Predicted polypeptide sequence is orthologous to G24 481 DNAGlycine max Predicted polypeptide sequence is orthologous to G24 482 DNAGlycine max Predicted polypeptide sequence is orthologous to G24 483 DNAGlycine max Predicted polypeptide sequence is orthologous to G24 484 DNAGlycine max Predicted polypeptide sequence is orthologous to G24 485 DNAGlycine max Predicted polypeptide sequence is orthologous to G24 486 DNAOryza sativa Predicted polypeptide sequence is orthologous to G24 487DNA Zea mays Predicted polypeptide sequence is orthologous to G24 488PRT Oryza sativa Orthologous to G24 489 PRT Oryza sativa Orthologous toG24 490 PRT Oryza sativa Orthologous to G24 491 DNA Glycine maxPredicted polypeptide sequence is orthologous to G28 492 DNA Glycine maxPredicted polypeptide sequence is orthologous to G28 493 DNA Glycine maxPredicted polypeptide sequence is orthologous to G28 494 DNA Glycine maxPredicted polypeptide sequence is orthologous to G28 495 DNA Glycine maxPredicted polypeptide sequence is orthologous to G28 496 DNA Glycine maxPredicted polypeptide sequence is orthologous to G28 497 DNA Glycine maxPredicted polypeptide sequence is orthologous to G28 498 DNA Glycine maxPredicted polypeptide sequence is orthologous to G28 499 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G28 500 DNA Zeamays Predicted polypeptide sequence is orthologous to G28 501 PRT Oryzasativa Orthologous to G28 502 PRT Oryza sativa Orthologous to G28 503PRT Mesembryanthemum Orthologous to G28 crystallinum 504 DNA Glycine maxPredicted polypeptide sequence is orthologous to G47, G2133 505 PRTOryza sativa Orthologous to G47, G2133 506 DNA Glycine max Predictedpolypeptide sequence is orthologous to G157, G859, G1842, G1843 507 DNAGlycine max Predicted polypeptide sequence is orthologous to G175, G877508 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG175, G877 509 DNA Zea mays Predicted polypeptide sequence isorthologous to G175, G877 510 DNA Zea mays Predicted polypeptidesequence is orthologous to G175, G877 511 DNA Zea mays Predictedpolypeptide sequence is orthologous to G175, G877 512 PRT Oryza sativaOrthologous to G175, G877 513 PRT Oryza sativa Orthologous to G175, G877514 PRT Oryza sativa Orthologous to G175, G877 515 PRT Nicotiana tabacumOrthologous to G175, G877 516 DNA Glycine max Predicted polypeptidesequence is orthologous to G180 517 DNA Glycine max Predictedpolypeptide sequence is orthologous to G180 518 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G180 519 DNA Zea maysPredicted polypeptide sequence is orthologous to G180 520 DNA Solanumtuberosum Predicted polypeptide sequence is orthologous to G180 521 PRTOryza sativa Orthologous to G180 522 PRT Capsella rubella Orthologous toG183 523 DNA Glycine max Predicted polypeptide sequence is orthologousto G188 524 DNA Zea mays Predicted polypeptide sequence is orthologousto G188 525 PRT Oryza sativa Orthologous to G188 526 PRT Oryza sativaOrthologous to G188 527 DNA Glycine max Predicted polypeptide sequenceis orthologous to G189 528 PRT Nicotiana tabacum Orthologous to G189 529DNA Glycine max Predicted polypeptide sequence is orthologous to G192530 PRT Oryza sativa Orthologous to G192 531 DNA Glycine max Predictedpolypeptide sequence is orthologous to G196 532 DNA Zea mays Predictedpolypeptide sequence is orthologous to G196 533 DNA Zea mays Predictedpolypeptide sequence is orthologous to G196 534 PRT Oryza sativaOrthologous to G196 535 PRT Oryza sativa Orthologous to G196 536 PRTOryza sativa Orthologous to G196 537 PRT Oryza sativa Orthologous toG196 538 DNA Glycine max Predicted polypeptide sequence is orthologousto G211 539 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G211 540 PRT Oryza sativa Orthologous to G211 541 DNAGlycine max Predicted polypeptide sequence is orthologous to G214, G680542 DNA Glycine max Predicted polypeptide sequence is orthologous toG214, G680 543 DNA Glycine max Predicted polypeptide sequence isorthologous to G214, G680 544 DNA Glycine max Predicted polypeptidesequence is orthologous to G214, G680 545 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G214, G680 546 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G214, G680 547 DNA Zeamays Predicted polypeptide sequence is orthologous to G214, G680 548 DNAZea mays Predicted polypeptide sequence is orthologous to G214, G680 549DNA Zea mays Predicted polypeptide sequence is orthologous to G214, G680550 DNA Glycine max Predicted polypeptide sequence is orthologous toG226, G682 551 DNA Glycine max Predicted polypeptide sequence isorthologous to G226 552 DNA Glycine max Predicted polypeptide sequenceis orthologous to G226, G682 553 DNA Glycine max Predicted polypeptidesequence is orthologous to G226, G682 554 DNA Glycine max Predictedpolypeptide sequence is orthologous to G226, G682 555 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G226, G682 556 DNA Zeamays Predicted polypeptide sequence is orthologous to G226, G682 557 DNAZea mays Predicted polypeptide sequence is orthologous to G226, G682 558PRT Oryza sativa Orthologous to G226, G682 559 PRT Oryza sativaOrthologous to G226, G682 560 DNA Glycine max Predicted polypeptidesequence is orthologous to G241 561 DNA Glycine max Predictedpolypeptide sequence is orthologous to G241 562 DNA Glycine maxPredicted polypeptide sequence is orthologous to G241 563 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G241 564 DNA Zeamays Predicted polypeptide sequence is orthologous to G241 565 DNA Zeamays Predicted polypeptide sequence is orthologous to G241 566 DNA Zeamays Predicted polypeptide sequence is orthologous to G241 567 DNA Zeamays Predicted polypeptide sequence is orthologous to G241 568 DNA Zeamays Predicted polypeptide sequence is orthologous to G241 569 PRTNicotiana tabacum Orthologous to G241 570 DNA Glycine max Predictedpolypeptide sequence is orthologous to G254 571 DNA Glycine maxPredicted polypeptide sequence is orthologous to G256 572 DNA Glycinemax Predicted polypeptide sequence is orthologous to G256 573 DNAGlycine max Predicted polypeptide sequence is orthologous to G256 574DNA Glycine max Predicted polypeptide sequence is orthologous to G256575 DNA Glycine max Predicted polypeptide sequence is orthologous toG256 576 DNA Glycine max Predicted polypeptide sequence is orthologousto G256 577 DNA Glycine max Predicted polypeptide sequence isorthologous to G256 578 DNA Oryza sativa Predicted polypeptide sequenceis orthologous to G256 579 DNA Zea mays Predicted polypeptide sequenceis orthologous to G256 580 DNA Zea mays Predicted polypeptide sequenceis orthologous to G256 581 DNA Zea mays Predicted polypeptide sequenceis orthologous to G256 582 DNA Zea mays Predicted polypeptide sequenceis orthologous to G256 583 DNA Zea mays Predicted polypeptide sequenceis orthologous to G256 584 DNA Zea mays Predicted polypeptide sequenceis orthologous to G256 585 G3500 DNA Lycopersicon Predicted polypeptidesequence is esculentum orthologous to G256 586 G3501 DNA LycopersiconPredicted polypeptide sequence is esculentum orthologous to G256 587G3385 PRT Oryza sativa Orthologous to G256 588 G3386 PRT Oryza sativaOrthologous to G256 589 PRT Oryza sativa Orthologous to G256 590 G3384PRT Oryza sativa Orthologous to G256 591 PRT Oryza sativa Orthologous toG256 592 G3502 PRT Oryza sativa japonica Orthologous to G256 593 G3500PRT Lycopersicon Orthologous to G256 esculentum 594 G3501 PRTLycopersicon Orthologous to G256 esculentum 595 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G278 596 DNA Zea maysPredicted polypeptide sequence is orthologous to G278 597 PRT Oryzasativa Orthologous to G278 598 DNA Glycine max Predicted polypeptidesequence is orthologous to G312 599 DNA Zea mays Predicted polypeptidesequence is orthologous to G312 600 DNA Euphorbia esula Predictedpolypeptide sequence is orthologous to G312 601 DNA Glycine maxPredicted polypeptide sequence is orthologous to G325 602 DNA Glycinemax Predicted polypeptide sequence is orthologous to G343 603 DNAGlycine max Predicted polypeptide sequence is orthologous to G343 604DNA Glycine max Predicted polypeptide sequence is orthologous to G343605 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG343 606 DNA Oryza sativa Predicted polypeptide sequence is orthologousto G343 607 PRT Oryza sativa Orthologous to G343 608 PRT Oryza sativaOrthologous to G343 609 PRT Oryza sativa Orthologous to G343 610 DNAGlycine max Predicted polypeptide sequence is orthologous to G353, G354611 DNA Glycine max Predicted polypeptide sequence is orthologous toG353, G354 612 DNA Glycine max Predicted polypeptide sequence isorthologous to G353, G354 613 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G353, G354 614 DNA Zea mays Predictedpolypeptide sequence is orthologous to G353, G354 615 DNA Zea maysPredicted polypeptide sequence is orthologous to G353, G354 616 DNA Zeamays Predicted polypeptide sequence is orthologous to G353, G354 617 DNAZea mays Predicted polypeptide sequence is orthologous to G353, G354 618DNA Zea mays Predicted polypeptide sequence is orthologous to G353, G354619 DNA Zea mays Predicted polypeptide sequence is orthologous to G353,G354 620 DNA Zea mays Predicted polypeptide sequence is orthologous toG353, G354 621 PRT Oryza sativa Orthologous to G353, G354 622 PRT Oryzasativa Orthologous to G353, G354 623 PRT Oryza sativa Orthologous toG353, G354 624 PRT Oryza sativa Orthologous to G353, G354 625 PRT Oryzasativa Orthologous to G353, G354 626 PRT Oryza sativa Orthologous toG353, G354 627 DNA Glycine max Predicted polypeptide sequence isorthologous to G361, G362 628 DNA Glycine max Predicted polypeptidesequence is orthologous to G361, G362 629 DNA Glycine max Predictedpolypeptide sequence is orthologous to G361 630 DNA Glycine maxPredicted polypeptide sequence is orthologous to G361, G362 631 DNAGlycine max Predicted polypeptide sequence is orthologous to G361, G362632 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG361, G362 633 DNA Zea mays Predicted polypeptide sequence isorthologous to G361, G362 634 DNA Zea mays Predicted polypeptidesequence is orthologous to G361, G362 635 PRT Oryza sativa Orthologousto G361, G362 636 PRT Oryza sativa Orthologous to G361, G362 637 PRTOryza sativa Orthologous to G361, G362 638 PRT Oryza sativa Orthologousto G361, G362 639 PRT Oryza sativa Orthologous to G361, G362 640 DNAGlycine max Predicted polypeptide sequence is orthologous to G390, G391,G438 641 DNA Glycine max Predicted polypeptide sequence is orthologousto G390, G391, G438 642 DNA Glycine max Predicted polypeptide sequenceis orthologous to G390, G391, G438 643 DNA Glycine max Predictedpolypeptide sequence is orthologous to G390, G391, G438 644 DNA Glycinemax Predicted polypeptide sequence is orthologous to G390, G391, G438645 DNA Glycine max Predicted polypeptide sequence is orthologous toG390, G391, G438 646 DNA Glycine max Predicted polypeptide sequence isorthologous to G390, G391, G438 647 DNA Glycine max Predictedpolypeptide sequence is orthologous to G390, G391 648 DNA Glycine maxPredicted polypeptide sequence is orthologous to G390, G391, G438 649DNA Glycine max Predicted polypeptide sequence is orthologous to G390,G391, G438 650 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G390 651 DNA Oryza sativa Predicted polypeptide sequenceis orthologous to G390, G438 652 DNA Zea mays Predicted polypeptidesequence is orthologous to G390, G391, G438 653 DNA Zea mays Predictedpolypeptide sequence is orthologous to G390, G391, G438 654 DNA Zea maysPredicted polypeptide sequence is orthologous to G390, G391, G438 655DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391656 DNA Zea mays Predicted polypeptide sequence is orthologous to G390,G391, G438 657 DNA Zea mays Predicted polypeptide sequence isorthologous to G390, G391, G438 658 DNA Zea mays Predicted polypeptidesequence is orthologous to G390, G391, G438 659 DNA Zea mays Predictedpolypeptide sequence is orthologous to G390, G391, G438 660 DNA Zea maysPredicted polypeptide sequence is orthologous to G390, G391, G438 661DNA Zea mays Predicted polypeptide sequence is orthologous to G390,G391, G438 662 DNA Zea mays Predicted polypeptide sequence isorthologous to G390, G391, G438 663 DNA Lycopersicon Predictedpolypeptide sequence is esculentum orthologous to G390, G391, G438 664DNA Oryza sativa Predicted polypeptide sequence is orthologous to G391,G438 665 PRT Oryza sativa Orthologous to G390, G391, G438 666 PRT Oryzasativa Orthologous to G390, G391, G438 667 PRT Oryza sativa Orthologousto G390, G391, G438 668 PRT Oryza sativa Orthologous to G390, G391, G438669 PRT Physcomitrella patens Orthologous to G391 670 DNA Glycine maxPredicted polypeptide sequence is orthologous to G409 671 DNA Glycinemax Predicted polypeptide sequence is orthologous to G409 672 DNAGlycine max Predicted polypeptide sequence is orthologous to G409 673DNA Glycine max Predicted polypeptide sequence is orthologous to G409674 DNA Glycine max Predicted polypeptide sequence is orthologous toG409 675 DNA Glycine max Predicted polypeptide sequence is orthologousto G409 676 DNA Glycine max Predicted polypeptide sequence isorthologous to G409 677 DNA Glycine max Predicted polypeptide sequenceis orthologous to G409 678 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G409 679 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G409 680 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G409 681 DNA Zea maysPredicted polypeptide sequence is orthologous to G409 682 DNA Zea maysPredicted polypeptide sequence is orthologous to G409 683 DNA Zea maysPredicted polypeptide sequence is orthologous to G409 684 DNA Zea maysPredicted polypeptide sequence is orthologous to G409 685 DNA Zea maysPredicted polypeptide sequence is orthologous to G409 686 DNA Zea maysPredicted polypeptide sequence is orthologous to G409 687 DNA Zea maysPredicted polypeptide sequence is orthologous to G409 688 DNA Glycinemax Predicted polypeptide sequence is orthologous to G427 689 DNAGlycine max Predicted polypeptide sequence is orthologous to G427 690DNA Glycine max Predicted polypeptide sequence is orthologous to G427691 DNA Glycine max Predicted polypeptide sequence is orthologous toG427 692 DNA Glycine max Predicted polypeptide sequence is orthologousto G427 693 DNA Glycine max Predicted polypeptide sequence isorthologous to G427 694 DNA Glycine max Predicted polypeptide sequenceis orthologous to G427 695 DNA Glycine max Predicted polypeptidesequence is orthologous to G427 696 DNA Glycine max Predictedpolypeptide sequence is orthologous to G427 697 DNA Glycine maxPredicted polypeptide sequence is orthologous to G427 698 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G427 699 DNA Zeamays Predicted polypeptide sequence is orthologous to G427 700 DNA Zeamays Predicted polypeptide sequence is orthologous to G427 701 DNA Zeamays Predicted polypeptide sequence is orthologous to G427 702 DNA Zeamays Predicted polypeptide sequence is orthologous to G427 703 DNA Zeamays Predicted polypeptide sequence is orthologous to G427 704 DNA Zeamays Predicted polypeptide sequence is orthologous to G427 705 DNA Zeamays Predicted polypeptide sequence is orthologous to G427 706 DNA Zeamays Predicted polypeptide sequence is orthologous to G427 707 DNA Zeamays Predicted polypeptide sequence is orthologous to G427 708 PRT Oryzasativa Orthologous to G427 709 PRT Oryza sativa Orthologous to G427 710PRT Oryza sativa Orthologous to G427 711 PRT Malus × domesticaOrthologous to G427 712 PRT Nicotiana tabacum Orthologous to G427 713PRT Lycopersicon Orthologous to G427 esculentum 714 DNA Glycine maxPredicted polypeptide sequence is orthologous to G438 715 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G438 716 DNAOryza sativa Predicted polypeptide sequence is orthologous to G438 717DNA Oryza sativa Predicted polypeptide sequence is orthologous to G438718 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG438 719 DNA Zea mays Predicted polypeptide sequence is orthologous toG438 720 PRT Physcomitrella patens Orthologous to G438 721 PRT Oryzasativa Orthologous to G438 722 DNA Glycine max Predicted polypeptidesequence is orthologous to G450 723 DNA Glycine max Predictedpolypeptide sequence is orthologous to G450 724 DNA Glycine maxPredicted polypeptide sequence is orthologous to G450 725 DNA Glycinemax Predicted polypeptide sequence is orthologous to G450 726 DNAGlycine max Predicted polypeptide sequence is orthologous to G450 727DNA Glycine max Predicted polypeptide sequence is orthologous to G450728 DNA Glycine max Predicted polypeptide sequence is orthologous toG450 729 DNA Glycine max Predicted polypeptide sequence is orthologousto G450 730 DNA Glycine max Predicted polypeptide sequence isorthologous to G450 731 DNA Oryza sativa Predicted polypeptide sequenceis orthologous to G450 732 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G450 733 DNA Zea mays Predicted polypeptidesequence is orthologous to G450 734 DNA Zea mays Predicted polypeptidesequence is orthologous to G450 735 DNA Zea mays Predicted polypeptidesequence is orthologous to G450 736 PRT Oryza sativa Orthologous to G450737 PRT Oryza sativa Orthologous to G450 738 PRT Oryza sativaOrthologous to G450 739 PRT Oryza sativa Orthologous to G450 740 DNAOryza sativa Predicted polypeptide sequence is orthologous to G464 741DNA Zea mays Predicted polypeptide sequence is orthologous to G464 742PRT Oryza sativa Orthologous to G464 743 DNA Glycine max Predictedpolypeptide sequence is orthologous to G470 744 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G470 745 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G470 746 DNAGlycine max Predicted polypeptide sequence is orthologous to G481, G482747 DNA Glycine max Predicted polypeptide sequence is orthologous toG481, G482 748 DNA Glycine max Predicted polypeptide sequence isorthologous to G481, G482 749 DNA Glycine max Predicted polypeptidesequence is orthologous to G481, G482 750 DNA Glycine max Predictedpolypeptide sequence is orthologous to G481, G482 751 DNA Glycine maxPredicted polypeptide sequence is orthologous to G481, G482 752 DNAGlycine max Predicted polypeptide sequence is orthologous to G481, G482753 DNA Glycine max Predicted polypeptide sequence is orthologous toG481, G482 754 DNA Glycine max Predicted polypeptide sequence isorthologous to G481 755 DNA Glycine max Predicted polypeptide sequenceis orthologous to G481 756 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G481 757 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G481, G482 758 DNA Zea maysPredicted polypeptide sequence is orthologous to G481 759 DNA Zea maysPredicted polypeptide sequence is orthologous to G481, G482 760 DNA Zeamays Predicted polypeptide sequence is orthologous to G481, G482 761 DNAZea mays Predicted polypeptide sequence is orthologous to G481, G482 762DNA Zea mays Predicted polypeptide sequence is orthologous to G481, G482763 DNA Zea mays Predicted polypeptide sequence is orthologous to G481,G482 764 DNA Zea mays Predicted polypeptide sequence is orthologous toG481, G482 765 DNA Zea mays Predicted polypeptide sequence isorthologous to G481, G482 766 DNA Zea mays Predicted polypeptidesequence is orthologous to G481, G482 767 DNA Zea mays Predictedpolypeptide sequence is orthologous to G481, G482 768 DNA Gossypiumarboreum Predicted polypeptide sequence is orthologous to G481, G482 769DNA Glycine max Predicted polypeptide sequence is orthologous to G481,G482 770 DNA Gossypium hirsutum Predicted polypeptide sequence isorthologous to G481, G482 771 DNA Lycopersicon Predicted polypeptidesequence is esculentum orthologous to G481, G482 772 DNA LycopersiconPredicted polypeptide sequence is esculentum orthologous to G481, G482773 DNA Medicago truncatula Predicted polypeptide sequence isorthologous to G481, G482 774 DNA Lycopersicon Predicted polypeptidesequence is esculentum orthologous to G481, G482 775 DNA Solanumtuberosum Predicted polypeptide sequence is orthologous to G481, G482776 DNA Triticum aestivum Predicted polypeptide sequence is orthologousto G481, G482 777 DNA Hordeum vulgare Predicted polypeptide sequence isorthologous to G481, G482 778 DNA Triticum monococcum Predictedpolypeptide sequence is orthologous to G481, G482 779 DNA Glycine maxPredicted polypeptide sequence is orthologous to G482 780 PRT Oryzasativa Orthologous to G481, G482 781 PRT Oryza sativa Orthologous toG481, G482 782 PRT Oryza sativa Orthologous to G481, G482 783 PRT Oryzasativa Orthologous to G481, G482 784 PRT Oryza sativa Orthologous toG481, G482 785 PRT Zea mays Orthologous to G481, G482 786 PRT Zea maysOrthologous to G481, G482 787 PRT Oryza sativa Orthologous to G481, G482788 PRT Oryza sativa Orthologous to G481, G482 789 PRT Oryza sativaOrthologous to G481, G482 790 PRT Oryza sativa Orthologous to G481, G482791 PRT Oryza sativa Orthologous to G481, G482 792 PRT Oryza sativaOrthologous to G481, G482 793 PRT Oryza sativa Orthologous to G481, G482794 PRT Oryza sativa Orthologous to G481, G482 795 PRT Oryza sativaOrthologous to G481, G482 796 PRT Oryza sativa Orthologous to G481, G482797 PRT Glycine max Orthologous to G481, G482 798 PRT Glycine maxOrthologous to G481, G482 799 PRT Glycine max Orthologous to G481, G482800 PRT Glycine max Orthologous to G481, G482 801 PRT Glycine maxOrthologous to G481, G482 802 PRT Glycine max Orthologous to G481, G482803 PRT Glycine max Orthologous to G481, G482 804 PRT Zea maysOrthologous to G481, G482 805 PRT Zea mays Orthologous to G481, G482 806PRT Zea mays Orthologous to G481, G482 807 PRT Zea mays Orthologous toG481, G482 808 DNA Glycine max Predicted polypeptide sequence isorthologous to G484 809 DNA Glycine max Predicted polypeptide sequenceis orthologous to G484 810 DNA Glycine max Predicted polypeptidesequence is orthologous to G484 811 DNA Glycine max Predictedpolypeptide sequence is orthologous to G484 812 DNA Glycine maxPredicted polypeptide sequence is orthologous to G484 813 DNA Glycinemax Predicted polypeptide sequence is orthologous to G484 814 DNAGlycine max Predicted polypeptide sequence is orthologous to G484 815DNA Glycine max Predicted polypeptide sequence is orthologous to G484816 DNA Glycine max Predicted polypeptide sequence is orthologous toG484 817 DNA Glycine max Predicted polypeptide sequence is orthologousto G484 818 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G484 819 DNA Zea mays Predicted polypeptide sequence isorthologous to G484 820 DNA Zea mays Predicted polypeptide sequence isorthologous to G484 821 DNA Zea mays Predicted polypeptide sequence isorthologous to G484 822 DNA Zea mays Predicted polypeptide sequence isorthologous to G484 823 DNA Zea mays Predicted polypeptide sequence isorthologous to G484 824 PRT Oryza sativa Orthologous to G484 825 DNAGlycine max Predicted polypeptide sequence is orthologous to G489 826DNA Glycine max Predicted polypeptide sequence is orthologous to G489827 DNA Glycine max Predicted polypeptide sequence is orthologous toG489 828 DNA Glycine max Predicted polypeptide sequence is orthologousto G489 829 DNA Glycine max Predicted polypeptide sequence isorthologous to G489 830 DNA Glycine max Predicted polypeptide sequenceis orthologous to G489 831 DNA Glycine max Predicted polypeptidesequence is orthologous to G489 832 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G489 833 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G489 834 DNA Zea maysPredicted polypeptide sequence is orthologous to G489 835 PRT Oryzasativa Orthologous to G489 836 PRT Oryza sativa Orthologous to G489 837PRT Oryza sativa Orthologous to G489 838 DNA Glycine max Predictedpolypeptide sequence is orthologous to G504 839 DNA Glycine maxPredicted polypeptide sequence is orthologous to G504 840 DNA Glycinemax Predicted polypeptide sequence is orthologous to G504 841 DNAGlycine max Predicted polypeptide sequence is orthologous to G504 842DNA Glycine max Predicted polypeptide sequence is orthologous to G504843 DNA Glycine max Predicted polypeptide sequence is orthologous toG504 844 DNA Glycine max Predicted polypeptide sequence is orthologousto G504 845 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G504 846 DNA Oryza sativa Predicted polypeptide sequenceis orthologous to G504 847 DNA Zea mays Predicted polypeptide sequenceis orthologous to G504 848 DNA Zea mays Predicted polypeptide sequenceis orthologous to G504 849 DNA Zea mays Predicted polypeptide sequenceis orthologous to G504 850 DNA Zea mays Predicted polypeptide sequenceis orthologous to G504 851 PRT Oryza sativa Orthologous to G504 852 PRTOryza sativa Orthologous to G504 853 PRT Oryza sativa Orthologous toG504 854 PRT Oryza sativa Orthologous to G504 855 DNA LycopersiconPredicted polypeptide sequence is esculentum orthologous to G509 856 DNAGlycine max Predicted polypeptide sequence is orthologous to G509 857DNA Glycine max Predicted polypeptide sequence is orthologous to G509858 DNA Glycine max Predicted polypeptide sequence is orthologous toG509 859 DNA Oryza sativa Predicted polypeptide sequence is orthologousto G509 860 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G509 861 DNA Zea mays Predicted polypeptide sequence isorthologous to G509 862 DNA Zea mays Predicted polypeptide sequence isorthologous to G509 863 DNA Zea mays Predicted polypeptide sequence isorthologous to G509 864 DNA Zea mays Predicted polypeptide sequence isorthologous to G509 865 PRT Oryza sativa Orthologous to G509 866 PRTOryza sativa Orthologous to G509 867 PRT Oryza sativa Orthologous toG509 868 DNA Glycine max Predicted polypeptide sequence is orthologousto G519 869 DNA Glycine max Predicted polypeptide sequence isorthologous to G519 870 DNA Glycine max Predicted polypeptide sequenceis orthologous to G519 871 DNA Glycine max Predicted polypeptidesequence is orthologous to G519 872 DNA Glycine max Predictedpolypeptide sequence is orthologous to G519 873 DNA Glycine maxPredicted polypeptide sequence is orthologous to G519 874 DNA Glycinemax Predicted polypeptide sequence is orthologous to G519 875 DNAGlycine max Predicted polypeptide sequence is orthologous to G519 876DNA Glycine max Predicted polypeptide sequence is orthologous to G519877 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG519 878 DNA Oryza sativa Predicted polypeptide sequence is orthologousto G519 879 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G519 880 DNA Zea mays Predicted polypeptide sequence isorthologous to G519 881 DNA Zea mays Predicted polypeptide sequence isorthologous to G519 882 DNA Zea mays Predicted polypeptide sequence isorthologous to G519 883 DNA Zea mays Predicted polypeptide sequence isorthologous to G519 884 DNA Zea mays Predicted polypeptide sequence isorthologous to G519 885 DNA Zea mays Predicted polypeptide sequence isorthologous to G519 886 DNA Zea mays Predicted polypeptide sequence isorthologous to G519 887 DNA Zea mays Predicted polypeptide sequence isorthologous to G519 888 DNA Zea mays Predicted polypeptide sequence isorthologous to G519 889 DNA Zea mays Predicted polypeptide sequence isorthologous to G519 890 PRT Oryza sativa Orthologous to G519 891 PRTOryza sativa Orthologous to G519 892 DNA Glycine max Predictedpolypeptide sequence is orthologous to G545 893 DNA Glycine maxPredicted polypeptide sequence is orthologous to G545 894 DNA Glycinemax Predicted polypeptide sequence is orthologous to G545 895 DNAGlycine max Predicted polypeptide sequence is orthologous to G545 896DNA Glycine max Predicted polypeptide sequence is orthologous to G545897 DNA Glycine max Predicted polypeptide sequence is orthologous toG545 898 DNA Glycine max Predicted polypeptide sequence is orthologousto G545 899 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G545 900 DNA Zea mays Predicted polypeptide sequence isorthologous to G545 901 DNA Zea mays Predicted polypeptide sequence isorthologous to G545 902 DNA Zea mays Predicted polypeptide sequence isorthologous to G545 903 PRT Oryza sativa Orthologous to G545 904 PRTOryza sativa Orthologous to G545 905 PRT Oryza sativa Orthologous toG545 906 PRT Oryza sativa Orthologous to G545 907 PRT Datisca glomerataOrthologous to G545 908 DNA Oryza sativa Predicted polypeptide sequenceis orthologous to G546 909 DNA Zea mays Predicted polypeptide sequenceis orthologous to G561 910 PRT Sinapis alba Orthologous to G561 911 PRTRaphanus sativus Orthologous to G561 912 PRT Brassica napus Orthologousto G561 913 PRT Brassica napus Orthologous to G561 914 DNA Glycine maxPredicted polypeptide sequence is orthologous to G562 915 DNA Glycinemax Predicted polypeptide sequence is orthologous to G562 916 DNAGlycine max Predicted polypeptide sequence is orthologous to G562 917DNA Glycine max Predicted polypeptide sequence is orthologous to G562918 DNA Glycine max Predicted polypeptide sequence is orthologous toG562 919 DNA Zea mays Predicted polypeptide sequence is orthologous toG562 920 DNA Zea mays Predicted polypeptide sequence is orthologous toG562 921 DNA Zea mays Predicted polypeptide sequence is orthologous toG562 922 PRT Oryza sativa Orthologous to G562 923 PRT Oryza sativaOrthologous to G562 924 DNA Glycine max Predicted polypeptide sequenceis orthologous to G567 925 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G567 926 PRT Oryza sativa Orthologous to G567927 DNA Glycine max Predicted polypeptide sequence is orthologous toG568 928 DNA Glycine max Predicted polypeptide sequence is orthologousto G568 929 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G568 930 DNA Oryza sativa Predicted polypeptide sequenceis orthologous to G568 931 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G568 932 DNA Zea mays Predicted polypeptidesequence is orthologous to G568 933 PRT Oryza sativa Orthologous to G568934 PRT Populus balsamifera Orthologous to G568 subsp. trichocarpa ×Populus deltoides 935 DNA Glycine max Predicted polypeptide sequence isorthologous to G584 936 DNA Glycine max Predicted polypeptide sequenceis orthologous to G584 937 DNA Glycine max Predicted polypeptidesequence is orthologous to G584 938 DNA Glycine max Predictedpolypeptide sequence is orthologous to G584 939 DNA Glycine maxPredicted polypeptide sequence is orthologous to G584 940 DNA Zea maysPredicted polypeptide sequence is orthologous to G584 941 DNA Zea maysPredicted polypeptide sequence is orthologous to G584 942 DNA Zea maysPredicted polypeptide sequence is orthologous to G584 943 PRT Oryzasativa Orthologous to G584 944 DNA Glycine max Predicted polypeptidesequence is orthologous to G585 945 DNA Glycine max Predictedpolypeptide sequence is orthologous to G585 946 DNA Glycine maxPredicted polypeptide sequence is orthologous to G585 947 DNA Glycinemax Predicted polypeptide sequence is orthologous to G585 948 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G585 949 DNA Zeamays Predicted polypeptide sequence is orthologous to G585 950 DNA Zeamays Predicted polypeptide sequence is orthologous to G585 951 DNA Zeamays Predicted polypeptide sequence is orthologous to G585 952 DNA Zeamays Predicted polypeptide sequence is orthologous to G585 953 PRT Oryzasativa Orthologous to G585 954 PRT Oryza sativa Orthologous to G585 955PRT Oryza sativa Orthologous to G585 956 PRT Oryza sativa Orthologous toG585 957 PRT Oryza sativa Orthologous to G585 958 PRT Oryza sativaOrthologous to G585 959 PRT Gossypium hirsutum Orthologous to G585 960PRT Antirrhinum majus Orthologous to G585 961 DNA Glycine max Predictedpolypeptide sequence is orthologous to G590 962 DNA Glycine maxPredicted polypeptide sequence is orthologous to G590 963 DNA Glycinemax Predicted polypeptide sequence is orthologous to G590 964 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G590 965 DNA Zeamays Predicted polypeptide sequence is orthologous to G590 966 PRT Oryzasativa Orthologous to G590 967 PRT Oryza sativa Orthologous to G590 968DNA Oryza sativa Predicted polypeptide sequence is orthologous to G597969 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG597 970 DNA Oryza sativa Predicted polypeptide sequence is orthologousto G597 971 DNA Zea mays Predicted polypeptide sequence is orthologousto G597 972 DNA Zea mays Predicted polypeptide sequence is orthologousto G597 973 DNA Zea mays Predicted polypeptide sequence is orthologousto G597 974 DNA Zea mays Predicted polypeptide sequence is orthologousto G597 975 DNA Zea mays Predicted polypeptide sequence is orthologousto G597 976 DNA Zea mays Predicted polypeptide sequence is orthologousto G597 977 DNA Zea mays Predicted polypeptide sequence is orthologousto G597 978 DNA Zea mays Predicted polypeptide sequence is orthologousto G597 979 DNA Zea mays Predicted polypeptide sequence is orthologousto G597 980 DNA Zea mays Predicted polypeptide sequence is orthologousto G597 981 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G634 982 DNA Oryza sativa Predicted polypeptide sequenceis orthologous to G634 983 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G634 984 DNA Zea mays Predicted polypeptidesequence is orthologous to G634 985 DNA Zea mays Predicted polypeptidesequence is orthologous to G634 986 DNA Zea mays Predicted polypeptidesequence is orthologous to G634 987 PRT Oryza sativa Orthologous to G634988 PRT Oryza sativa Orthologous to G634 989 DNA Glycine max Predictedpolypeptide sequence is orthologous to G635 990 DNA Glycine maxPredicted polypeptide sequence is orthologous to G635 991 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G635 992 DNAOryza sativa Predicted polypeptide sequence is orthologous to G635 993DNA Zea mays Predicted polypeptide sequence is orthologous to G635 994PRT Oryza sativa Orthologous to G635 995 DNA Glycine max Predictedpolypeptide sequence is orthologous to G636 996 DNA Glycine maxPredicted polypeptide sequence is orthologous to G636 997 DNA Glycinemax Predicted polypeptide sequence is orthologous to G636 998 DNAGlycine max Predicted polypeptide sequence is orthologous to G636 999DNA Glycine max Predicted polypeptide sequence is orthologous to G6361000 DNA Glycine max Predicted polypeptide sequence is orthologous toG636 1001 DNA Glycine max Predicted polypeptide sequence is orthologousto G636 1002 DNA Glycine max Predicted polypeptide sequence isorthologous to G636 1003 DNA Oryza sativa Predicted polypeptide sequenceis orthologous to G636 1004 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G636 1005 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G636 1006 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G636 1007 DNA Zea maysPredicted polypeptide sequence is orthologous to G636 1008 DNA Zea maysPredicted polypeptide sequence is orthologous to G636 1009 DNA Zea maysPredicted polypeptide sequence is orthologous to G636 1010 DNA Zea maysPredicted polypeptide sequence is orthologous to G636 1011 PRT Pisumsativum Orthologous to G636 1012 DNA Glycine max Predicted polypeptidesequence is orthologous to G638 1013 DNA Glycine max Predictedpolypeptide sequence is orthologous to G638 1014 DNA Glycine maxPredicted polypeptide sequence is orthologous to G638 1015 DNA Glycinemax Predicted polypeptide sequence is orthologous to G638 1016 DNAMedicago truncatula Predicted polypeptide sequence is orthologous toG638 1017 DNA Glycine max Predicted polypeptide sequence is orthologousto G652 1018 DNA Glycine max Predicted polypeptide sequence isorthologous to G652 1019 DNA Glycine max Predicted polypeptide sequenceis orthologous to G652 1020 DNA Glycine max Predicted polypeptidesequence is orthologous to G652 1021 DNA Glycine max Predictedpolypeptide sequence is orthologous to G652 1022 DNA Glycine maxPredicted polypeptide sequence is orthologous to G652 1023 DNA Glycinemax Predicted polypeptide sequence is orthologous to G652 1024 DNAGlycine max Predicted polypeptide sequence is orthologous to G652 1025DNA Oryza sativa Predicted polypeptide sequence is orthologous to G6521026 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG652 1027 DNA Oryza sativa Predicted polypeptide sequence is orthologousto G652 1028 DNA Zea mays Predicted polypeptide sequence is orthologousto G652 1029 DNA Zea mays Predicted polypeptide sequence is orthologousto G652 1030 DNA Zea mays Predicted polypeptide sequence is orthologousto G652 1031 DNA Zea mays Predicted polypeptide sequence is orthologousto G652 1032 DNA Zea mays Predicted polypeptide sequence is orthologousto G652 1033 DNA Zea mays Predicted polypeptide sequence is orthologousto G652 1034 DNA Zea mays Predicted polypeptide sequence is orthologousto G652 1035 PRT Oryza sativa Orthologous to G652 1036 PRT Oryza sativaOrthologous to G652 1037 PRT Oryza sativa Orthologous to G652 1038 PRTOryza sativa Orthologous to G652 1039 PRT Oryza sativa Orthologous toG652 1040 PRT Oryza sativa Orthologous to G652 1041 PRT Oryza sativaOrthologous to G652 1042 PRT Oryza sativa Orthologous to G652 1043 DNAGlycine max Predicted polypeptide sequence is orthologous to G663 1044DNA Glycine max Predicted polypeptide sequence is orthologous to G6641045 DNA Glycine max Predicted polypeptide sequence is orthologous toG664 1046 DNA Glycine max Predicted polypeptide sequence is orthologousto G664 1047 DNA Glycine max Predicted polypeptide sequence isorthologous to G664 1048 DNA Glycine max Predicted polypeptide sequenceis orthologous to G664 1049 DNA Glycine max Predicted polypeptidesequence is orthologous to G664 1050 DNA Glycine max Predictedpolypeptide sequence is orthologous to G664 1051 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G664 1052 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G664 1053 DNAOryza sativa Predicted polypeptide sequence is orthologous to G664 1054DNA Oryza sativa Predicted polypeptide sequence is orthologous to G6641055 DNA Zea mays Predicted polypeptide sequence is orthologous to G6641056 DNA Zea mays Predicted polypeptide sequence is orthologous to G6641057 DNA Zea mays Predicted polypeptide sequence is orthologous to G6641058 DNA Zea mays Predicted polypeptide sequence is orthologous to G6641059 DNA Zea mays Predicted polypeptide sequence is orthologous to G6641060 DNA Zea mays Predicted polypeptide sequence is orthologous to G6641061 DNA Zea mays Predicted polypeptide sequence is orthologous to G6641062 DNA Zea mays Predicted polypeptide sequence is orthologous to G6641063 G3509 DNA Lycopersicon Predicted polypeptide sequence is esculentumorthologous to G664 1064 G3506 PRT Oryza sativa Orthologous to G664 1065G3504 PRT Oryza sativa Orthologous to G664 1066 PRT Oryza sativaOrthologous to G664 1067 PRT Oryza sativa Orthologous to G664 1068 G3503PRT Oryza sativa indica Orthologous to G664 1069 G3505 PRT Oryza sativajaponica Orthologous to G664 1070 G3507 PRT Oryza sativa japonicaOrthologous to G664 1071 G3508 PRT Oryza sativa japonica Orthologous toG664 1072 G3509 PRT Lycopersicon Orthologous to G664 esculentum 1073 PRTHordeum vulgare Orthologous to G664 subsp. vulgare 1074 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G680 1075 DNA Zea maysPredicted polypeptide sequence is orthologous to G680 1076 DNA Glycinemax Predicted polypeptide sequence is orthologous to G682 1077 DNAHordeum vulgare Predicted polypeptide sequence is subsp. vulgareorthologous to G682 1078 DNA Populus tremula × Predicted polypeptidesequence is Populus tremuloides orthologous to G682 1079 DNA Triticumaestivum Predicted polypeptide sequence is orthologous to G682 1080 DNAGossypium arboreum Predicted polypeptide sequence is orthologous to G6821081 PRT Oryza sativa Orthologous to G682 1082 PRT Oryza sativaOrthologous to G682 1083 PRT Glycine max Orthologous to G682 1084 PRTGlycine max Orthologous to G682 1085 PRT Glycine max Orthologous to G6821086 PRT Glycine max Orthologous to G682 1087 PRT Glycine maxOrthologous to G682 1088 PRT Glycine max Orthologous to G682 1089 PRTZea mays Orthologous to G682 1090 PRT Zea mays Orthologous to G682 1091DNA Glycine max Predicted polypeptide sequence is orthologous to G715,G1646 1092 DNA Glycine max Predicted polypeptide sequence is orthologousto G715, G1646 1093 DNA Glycine max Predicted polypeptide sequence isorthologous to G715, G1646 1094 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G715, G1646 1095 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G715, G1646 1096 DNA Zea maysPredicted polypeptide sequence is orthologous to G715, G1646 1097 DNAZea mays Predicted polypeptide sequence is orthologous to G715, G16461098 DNA Zea mays Predicted polypeptide sequence is orthologous to G715,G1646 1099 DNA Zea mays Predicted polypeptide sequence is orthologous toG715, G1646 1100 DNA Zea mays Predicted polypeptide sequence isorthologous to G715, G1646 1101 DNA Zea mays Predicted polypeptidesequence is orthologous to G715, G1646 1102 DNA Zea mays Predictedpolypeptide sequence is orthologous to G715, G1646 1103 DNA Zea maysPredicted polypeptide sequence is orthologous to G715, G1646 1104 DNAZea mays Predicted polypeptide sequence is orthologous to G715, G16461105 PRT Oryza sativa Orthologous to G715, G1646 1106 PRT Oryza sativaOrthologous to G715, G1646 1107 PRT Oryza sativa Orthologous to G715,G1646 1108 PRT Oryza sativa Orthologous to G715, G1646 1109 PRT Oryzasativa Orthologous to G715, G1646 1110 PRT Oryza sativa Orthologous toG715, G1646 1111 DNA Glycine max Predicted polypeptide sequence isorthologous to G720 1112 DNA Glycine max Predicted polypeptide sequenceis orthologous to G720 1113 DNA Glycine max Predicted polypeptidesequence is orthologous to G720 1114 DNA Glycine max Predictedpolypeptide sequence is orthologous to G720 1115 DNA Medicago truncatulaPredicted polypeptide sequence is orthologous to G720 1116 DNALycopersicon Predicted polypeptide sequence is esculentum orthologous toG720 1117 DNA Lycopersicon Predicted polypeptide sequence is esculentumorthologous to G720 1118 DNA Lycopersicon Predicted polypeptide sequenceis esculentum orthologous to G720 1119 DNA Solanum tuberosum Predictedpolypeptide sequence is orthologous to G720 1120 DNA Glycine maxPredicted polypeptide sequence is orthologous to G736 1121 DNA Glycinemax Predicted polypeptide sequence is orthologous to G736 1122 PRT Oryzasativa Orthologous to G736 1123 DNA Glycine max Predicted polypeptidesequence is orthologous to G748 1124 DNA Glycine max Predictedpolypeptide sequence is orthologous to G748 1125 DNA Glycine maxPredicted polypeptide sequence is orthologous to G748 1126 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G748 1127 DNAOryza sativa Predicted polypeptide sequence is orthologous to G748 1128DNA Zea mays Predicted polypeptide sequence is orthologous to G748 1129PRT Oryza sativa Orthologous to G748 1130 PRT Oryza sativa Orthologousto G748 1131 PRT Oryza sativa Orthologous to G748 1132 PRT Oryza sativaOrthologous to G748 1133 PRT Cucurbita maxima Orthologous to G748 1134DNA Glycine max Predicted polypeptide sequence is orthologous to G789,G1494 1135 DNA Glycine max Predicted polypeptide sequence is orthologousto G789, G1494 1136 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G789 1137 DNA Oryza sativa Predicted polypeptide sequenceis orthologous to G789, G1494 1138 DNA Zea mays Predicted polypeptidesequence is orthologous to G789, G1494 1139 PRT Oryza sativa Orthologousto G789, G1494 1140 PRT Oryza sativa Orthologous to G789, G1494 1141 PRTOryza sativa Orthologous to G789, G1494 1142 DNA Glycine max Predictedpolypeptide sequence is orthologous to G801 1143 DNA Glycine maxPredicted polypeptide sequence is orthologous to G801 1144 DNA Zea maysPredicted polypeptide sequence is orthologous to G801 1145 DNA Glycinemax Predicted polypeptide sequence is orthologous to G849 1146 DNAGlycine max Predicted polypeptide sequence is orthologous to G849 1147DNA Glycine max Predicted polypeptide sequence is orthologous to G8491148 DNA Glycine max Predicted polypeptide sequence is orthologous toG849 1149 DNA Glycine max Predicted polypeptide sequence is orthologousto G849 1150 DNA Glycine max Predicted polypeptide sequence isorthologous to G849 1151 DNA Zea mays Predicted polypeptide sequence isorthologous to G849 1152 DNA Zea mays Predicted polypeptide sequence isorthologous to G849 1153 DNA Zea mays Predicted polypeptide sequence isorthologous to G849 1154 DNA Glycine max Predicted polypeptide sequenceis orthologous to G864 1155 DNA Glycine max Predicted polypeptidesequence is orthologous to G864 1156 DNA Zea mays Predicted polypeptidesequence is orthologous to G864 1157 PRT Oryza sativa Orthologous toG864 1158 PRT Oryza sativa Orthologous to G864 1159 DNA Glycine maxPredicted polypeptide sequence is orthologous to G867, G1930 1160 DNAGlycine max Predicted polypeptide sequence is orthologous to G867, G19301161 DNA Glycine max Predicted polypeptide sequence is orthologous toG867, G1930 1162 DNA Glycine max Predicted polypeptide sequence isorthologous to G867, G1930 1163 DNA Glycine max Predicted polypeptidesequence is orthologous to G867, G1930 1164 DNA Glycine max Predictedpolypeptide sequence is orthologous to G867 1165 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G867 1166 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G867, G1930 1167DNA Zea mays Predicted polypeptide sequence is orthologous to G867,G1930 1168 DNA Zea mays Predicted polypeptide sequence is orthologous toG867, G1930 1169 DNA Zea mays Predicted polypeptide sequence isorthologous to G867, G1930 1170 DNA Zea mays Predicted polypeptidesequence is orthologous to G867, G1930 1171 DNA Glycine max Predictedpolypeptide sequence is orthologous to G867, G1930 1172 DNAMesembryanthemum Predicted polypeptide sequence is crystallinumorthologous to G867, G1930 1173 DNA Lycopersicon Predicted polypeptidesequence is esculentum orthologous to G867, G1930 1174 DNA Solanumtuberosum Predicted polypeptide sequence is orthologous to G867, G19301175 DNA Hordeum vulgare Predicted polypeptide sequence is orthologousto G867, G1930 1176 PRT Oryza sativa Orthologous to G867, G1930 1177 PRTOryza sativa Orthologous to G867, G1930 1178 PRT Oryza sativaOrthologous to G867, G1930 1179 PRT Oryza sativa Orthologous to G867,G1930 1180 PRT Oryza sativa Orthologous to G867, G1930 1181 PRT Oryzasativa Orthologous to G867, G1930 1182 PRT Glycine max Orthologous toG867, G1930 1183 PRT Glycine max Orthologous to G867, G1930 1184 PRTGlycine max Orthologous to G867, G1930 1185 PRT Zea mays Orthologous toG867, G1930 1186 PRT Zea mays Orthologous to G867, G1930 1187 DNAGlycine max Predicted polypeptide sequence is orthologous to G869 1188DNA Glycine max Predicted polypeptide sequence is orthologous to G8691189 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG869 1190 DNA Zea mays Predicted polypeptide sequence is orthologous toG869 1191 PRT Oryza sativa Orthologous to G869 1192 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G877 1193 DNA Glycinemax Predicted polypeptide sequence is orthologous to G881 1194 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G881 1195 DNAOryza sativa Predicted polypeptide sequence is orthologous to G881 1196DNA Zea mays Predicted polypeptide sequence is orthologous to G881 1197DNA Zea mays Predicted polypeptide sequence is orthologous to G881 1198DNA Zea mays Predicted polypeptide sequence is orthologous to G881 1199DNA Zea mays Predicted polypeptide sequence is orthologous to G881 1200PRT Oryza sativa Orthologous to G881 1201 PRT Oryza sativa Orthologousto G892 1202 DNA Mentha × piperita Predicted polypeptide sequence isorthologous to G896 1203 DNA Glycine max Predicted polypeptide sequenceis orthologous to G910 1204 DNA Glycine max Predicted polypeptidesequence is orthologous to G912 1205 DNA Glycine max Predictedpolypeptide sequence is orthologous to G912 1206 DNA Glycine maxPredicted polypeptide sequence is orthologous to G912 1207 DNA Glycinemax Predicted polypeptide sequence is orthologous to G912 1208 DNAGlycine max Predicted polypeptide sequence is orthologous to G912 1209DNA Glycine max Predicted polypeptide sequence is orthologous to G9121210 DNA Glycine max Predicted polypeptide sequence is orthologous toG912 1211 DNA Oryza sativa Predicted polypeptide sequence is orthologousto G912 1212 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G912, G913 1213 DNA Zea mays Predicted polypeptidesequence is orthologous to G912 1214 DNA Zea mays Predicted polypeptidesequence is orthologous to G912 1215 DNA Zea mays Predicted polypeptidesequence is orthologous to G912, G913 1216 DNA Zea mays Predictedpolypeptide sequence is orthologous to G912 1217 DNA Zea mays Predictedpolypeptide sequence is orthologous to G912 1218 DNA Brassica napusPredicted polypeptide sequence is orthologous to G912, G913 1219 DNASolanum tuberosum Predicted polypeptide sequence is orthologous to G9121220 DNA Descurainia sophia Predicted polypeptide sequence isorthologous to G912 1221 PRT Oryza sativa Orthologous to G912 1222 PRTOryza sativa Orthologous to G912, G913 1223 PRT Oryza sativa Orthologousto G912, G913 1224 PRT Oryza sativa Orthologous to G912 1225 PRTBrassica napus Orthologous to G912 1226 PRT Nicotiana tabacumOrthologous to G912 1227 PRT Oryza sativa Orthologous to G912 1228 PRTOryza sativa Orthologous to G912 1229 PRT Oryza sativa Orthologous toG912 1230 PRT Oryza sativa Orthologous to G912 1231 PRT Oryza sativaOrthologous to G912 1232 PRT Oryza sativa Orthologous to G912 1233 PRTOryza sativa Orthologous to G912 1234 PRT Oryza sativa Orthologous toG912 1235 PRT Oryza sativa Orthologous to G912 1236 PRT Oryza sativaOrthologous to G912 1237 PRT Glycine max Orthologous to G912 1238 PRTGlycine max Orthologous to G912 1239 PRT Glycine max Orthologous to G9121240 PRT Glycine max Orthologous to G912 1241 PRT Glycine maxOrthologous to G912 1242 PRT Glycine max Orthologous to G912 1243 PRTGlycine max Orthologous to G912 1244 PRT Zea mays Orthologous to G9121245 PRT Zea mays Orthologous to G912 1246 PRT Zea mays Orthologous toG912 1247 PRT Zea mays Orthologous to G912 1248 PRT Zea mays Orthologousto G912 1249 DNA Glycine max Predicted polypeptide sequence isorthologous to G922 1250 DNA Glycine max Predicted polypeptide sequenceis orthologous to G922 1251 DNA Glycine max Predicted polypeptidesequence is orthologous to G922 1252 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G922 1253 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G922 1254 PRT Oryzasativa Orthologous to G922 1255 PRT Oryza sativa Orthologous to G9221256 PRT Oryza sativa Orthologous to G922 1257 PRT Oryza sativaOrthologous to G922 1258 DNA Glycine max Predicted polypeptide sequenceis orthologous to G926 1259 DNA Glycine max Predicted polypeptidesequence is orthologous to G926 1260 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G926 1261 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G926 1262 DNA Zea maysPredicted polypeptide sequence is orthologous to G926 1263 PRT Brassicanapus Orthologous to G926 1264 DNA Glycine max Predicted polypeptidesequence is orthologous to G961 1265 DNA Glycine max Predictedpolypeptide sequence is orthologous to G961 1266 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G961 1267 DNA Zea maysPredicted polypeptide sequence is orthologous to G961 1268 DNA Zea maysPredicted polypeptide sequence is orthologous to G961 1269 DNA Zea maysPredicted polypeptide sequence is orthologous to G961 1270 PRT Oryzasativa Orthologous to G961 1271 DNA Glycine max Predicted polypeptidesequence is orthologous to G974 1272 DNA Glycine max Predictedpolypeptide sequence is orthologous to G974 1273 DNA Glycine maxPredicted polypeptide sequence is orthologous to G974 1274 DNA Glycinemax Predicted polypeptide sequence is orthologous to G974 1275 DNAGlycine max Predicted polypeptide sequence is orthologous to G974 1276DNA Glycine max Predicted polypeptide sequence is orthologous to G9741277 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG974 1278 DNA Zea mays Predicted polypeptide sequence is orthologous toG974 1279 DNA Zea mays Predicted polypeptide sequence is orthologous toG974 1280 DNA Zea mays Predicted polypeptide sequence is orthologous toG974 1281 DNA Zea mays Predicted polypeptide sequence is orthologous toG974 1282 DNA Lycopersicon Predicted polypeptide sequence is esculentumorthologous to G974 1283 DNA Glycine max Predicted polypeptide sequenceis orthologous to G974 1284 DNA Solanum tuberosum Predicted polypeptidesequence is orthologous to G974 1285 DNA Poplar xylem Predictedpolypeptide sequence is orthologous to G974 1286 DNA Medicago truncatulaPredicted polypeptide sequence is orthologous to G974 1287 DNA Sorghumbicolor Predicted polypeptide sequence is orthologous to G974 1288 PRTOryza sativa Orthologous to G974 1289 PRT Oryza sativa Orthologous toG974 1290 PRT Oryza sativa Orthologous to G974 1291 PRT Atriplexhortensis Orthologous to G974 1292 DNA Glycine max Predicted polypeptidesequence is orthologous to G975, G2583 1293 DNA Glycine max Predictedpolypeptide sequence is orthologous to G975, G2583 1294 DNA Glycine maxPredicted polypeptide sequence is orthologous to G975, G2583 1295 DNAGlycine max Predicted polypeptide sequence is orthologous to G975, G25831296 DNA Glycine max Predicted polypeptide sequence is orthologous toG975, G2583 1297 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G975 1298 DNA Oryza sativa Predicted polypeptide sequenceis orthologous to G975, G2583 1299 DNA Zea mays Predicted polypeptidesequence is orthologous to G975, G2583 1300 DNA Zea mays Predictedpolypeptide sequence is orthologous to G975, G2583 1301 DNA Brassicarapa Predicted polypeptide sequence is orthologous to G975, G2583 1302PRT Oryza sativa Orthologous to G975, G2583 1303 DNA Glycine maxPredicted polypeptide sequence is orthologous to G979 1304 DNA Glycinemax Predicted polypeptide sequence is orthologous to G979 1305 DNAGlycine max Predicted polypeptide sequence is orthologous to G979 1306DNA Oryza sativa Predicted polypeptide sequence is orthologous to G9791307 DNA Zea mays Predicted polypeptide sequence is orthologous to G9791308 DNA Zea mays Predicted polypeptide sequence is orthologous to G9791309 DNA Zea mays Predicted polypeptide sequence is orthologous to G9791310 PRT Oryza sativa Orthologous to G979 1311 PRT Oryza sativaOrthologous to G979 1312 PRT Oryza sativa Orthologous to G979 1313 PRTOryza sativa Orthologous to G979 1314 PRT Oryza sativa Orthologous toG979 1315 DNA Glycine max Predicted polypeptide sequence is orthologousto G987 1316 DNA Glycine max Predicted polypeptide sequence isorthologous to G987 1317 DNA Glycine max Predicted polypeptide sequenceis orthologous to G987 1318 DNA Glycine max Predicted polypeptidesequence is orthologous to G987 1319 DNA Glycine max Predictedpolypeptide sequence is orthologous to G987 1320 DNA Glycine maxPredicted polypeptide sequence is orthologous to G987 1321 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G987 1322 DNAOryza sativa Predicted polypeptide sequence is orthologous to G987 1323DNA Zea mays Predicted polypeptide sequence is orthologous to G987 1324PRT Oryza sativa Orthologous to G987 1325 PRT Oryza sativa Orthologousto G988 1326 PRT Oryza sativa Orthologous to G988 1327 PRT Capsellarubella Orthologous to G988 1328 DNA Glycine max Predicted polypeptidesequence is orthologous to G1040 1329 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1040 1330 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1040 1331 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1040 1332 DNAGlycine max Predicted polypeptide sequence is orthologous to G1040 1333DNA Zea mays Predicted polypeptide sequence is orthologous to G1040 1334DNA Zea mays Predicted polypeptide sequence is orthologous to G1040 1335DNA Zea mays Predicted polypeptide sequence is orthologous to G1040 1336DNA Zea mays Predicted polypeptide sequence is orthologous to G1040 1337DNA Zea mays Predicted polypeptide sequence is orthologous to G1040 1338PRT Oryza sativa Orthologous to G1040 1339 PRT Oryza sativa Orthologousto G1040 1340 DNA Glycine max Predicted polypeptide sequence isorthologous to G1047 1341 DNA Zea mays Predicted polypeptide sequence isorthologous to G1047 1342 PRT Oryza sativa Orthologous to G1047 1343 PRTOryza sativa Orthologous to G1047 1344 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1051, G1052 1345 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1051, G1052 1346 DNAGlycine max Predicted polypeptide sequence is orthologous to G1051,G1052 1347 DNA Glycine max Predicted polypeptide sequence is orthologousto G1051, G1052 1348 DNA Glycine max Predicted polypeptide sequence isorthologous to G1051, G1052 1349 DNA Glycine max Predicted polypeptidesequence is orthologous to G1051, G1052 1350 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1051, G1052 1351 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1051, G10521352 DNA Zea mays Predicted polypeptide sequence is orthologous toG1051, G1052 1353 DNA Zea mays Predicted polypeptide sequence isorthologous to G1051, G1052 1354 DNA Zea mays Predicted polypeptidesequence is orthologous to G1051, G1052 1355 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1051, G1052 1356 DNA Zea maysPredicted polypeptide sequence is orthologous to G1051, G1052 1357 DNAZea mays Predicted polypeptide sequence is orthologous to G1051, G10521358 DNA Zea mays Predicted polypeptide sequence is orthologous toG1051, G1052 1359 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G1052 1360 DNA Zea mays Predicted polypeptide sequence isorthologous to G1052 1361 DNA Zea mays Predicted polypeptide sequence isorthologous to G1052 1362 PRT Oryza sativa Orthologous to G1051, G10521363 PRT Oryza sativa Orthologous to G1051, G1052 1364 PRT Oryza sativaOrthologous to G1051, G1052 1365 DNA Glycine max Predicted polypeptidesequence is orthologous to G1062 1366 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1062 1367 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1062 1368 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1062 1369 DNAOryza sativa Predicted polypeptide sequence is orthologous to G1062 1370DNA Oryza sativa Predicted polypeptide sequence is orthologous to G10621371 DNA Zea mays Predicted polypeptide sequence is orthologous to G10621372 DNA Zea mays Predicted polypeptide sequence is orthologous to G10621373 DNA Zea mays Predicted polypeptide sequence is orthologous to G10621374 DNA Zea mays Predicted polypeptide sequence is orthologous to G10621375 DNA Zea mays Predicted polypeptide sequence is orthologous to G10621376 DNA Medicago truncatula Predicted polypeptide sequence isorthologous to G1062 1377 DNA Lycopersicon Predicted polypeptidesequence is esculentum orthologous to G1062 1378 PRT Oryza sativaOrthologous to G1062 1379 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1063, G2143 1380 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1063, G2143 1381 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1063, G2143 1382 DNAGlycine max Predicted polypeptide sequence is orthologous to G1063,G2143 1383 DNA Glycine max Predicted polypeptide sequence is orthologousto G1063, G2143 1384 DNA Lycopersicon Predicted polypeptide sequence isesculentum orthologous to G1063, G2143 1385 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1064 1386 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1064 1387 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1064 1388 DNA Zeamays Predicted polypeptide sequence is orthologous to G1064 1389 DNA Zeamays Predicted polypeptide sequence is orthologous to G1064 1390 DNALycopersicon Predicted polypeptide sequence is esculentum orthologous toG1064 1391 PRT Oryza sativa Orthologous to G1064 1392 PRT Gossypiumhirsutum Orthologous to G1064 1393 DNA Glycine max Predicted polypeptidesequence is orthologous to G1069 1394 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1069 1395 PRT Oryza sativaOrthologous to G1069, G1073 1396 DNA Zea mays Predicted polypeptidesequence is orthologous to G1069 1397 DNA Lotus japonicus Predictedpolypeptide sequence is orthologous to G1069 1398 DNA LycopersiconPredicted polypeptide sequence is esculentum orthologous to G1073 1399PRT Oryza sativa Orthologous to G1073 1400 PRT Oryza sativa Orthologousto G1073 1401 PRT Oryza sativa Orthologous to G1073 1402 PRT Oryzasativa Orthologous to G1073 1403 PRT Oryza sativa Orthologous to G10731404 PRT Oryza sativa Orthologous to G1073 1405 PRT Oryza sativaOrthologous to G1073 1406 PRT Oryza sativa Orthologous to G1073 1407 PRTOryza sativa Orthologous to G1073 1408 PRT Oryza sativa Orthologous toG1073 1409 PRT Oryza sativa Orthologous to G1073 1410 PRT Oryza sativaOrthologous to G1073 1411 PRT Glycine max Orthologous to G1073 1412 PRTGlycine max Orthologous to G1073 1413 PRT Glycine max Orthologous toG1073 1414 PRT Glycine max Orthologous to G1073 1415 PRT Glycine maxOrthologous to G1073 1416 PRT Glycine max Orthologous to G1073 1417 PRTGlycine max Orthologous to G1073 1418 PRT Zea mays Orthologous to G10731419 DNA Glycine max Predicted polypeptide sequence is orthologous toG1075 1420 DNA Glycine max Predicted polypeptide sequence is orthologousto G1075 1421 DNA Glycine max Predicted polypeptide sequence isorthologous to G1075 1422 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1075 1423 DNA Glycine max Predicted polypeptidesequence is orthologous to G1075 1424 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G1075 1425 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G1075 1426 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1075 1427 DNAOryza sativa Predicted polypeptide sequence is orthologous to G1089 1428DNA Zea mays Predicted polypeptide sequence is orthologous to G1089 1429DNA Zea mays Predicted polypeptide sequence is orthologous to G1089 1430DNA Zea mays Predicted polypeptide sequence is orthologous to G1089 1431DNA Zea mays Predicted polypeptide sequence is orthologous to G1089 1432DNA Zea mays Predicted polypeptide sequence is orthologous to G1089 1433PRT Oryza sativa Orthologous to G1089 1434 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1134, G2555 1435 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1134, G2555 1436 DNAOryza sativa Predicted polypeptide sequence is orthologous to G1134,G2555 1437 DNA Glycine max Predicted polypeptide sequence is orthologousto G1140 1438 DNA Glycine max Predicted polypeptide sequence isorthologous to G1140 1439 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1140 1440 DNA Glycine max Predicted polypeptidesequence is orthologous to G1140 1441 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1140 1442 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1140 1443 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1140 1444 DNAZea mays Predicted polypeptide sequence is orthologous to G1140 1445 DNAZea mays Predicted polypeptide sequence is orthologous to G1140 1446 DNAZea mays Predicted polypeptide sequence is orthologous to G1140 1447 DNAZea mays Predicted polypeptide sequence is orthologous to G1140 1448 DNAZea mays Predicted polypeptide sequence is orthologous to G1140 1449 DNAZea mays Predicted polypeptide sequence is orthologous to G1140 1450 DNAZea mays Predicted polypeptide sequence is orthologous to G1140 1451 DNAZea mays Predicted polypeptide sequence is orthologous to G1140 1452 DNAZea mays Predicted polypeptide sequence is orthologous to G1140 1453 PRTOryza sativa Orthologous to G1140 1454 PRT Ipomoea batatas Orthologousto G1140 1455 DNA Zea mays Predicted polypeptide sequence is orthologousto G1146 1456 DNA Zea mays Predicted polypeptide sequence is orthologousto G1146 1457 PRT Oryza sativa Orthologous to G1146 1458 PRT Oryzasativa Orthologous to G1146 1459 PRT Oryza sativa Orthologous to G11461460 DNA Glycine max Predicted polypeptide sequence is orthologous toG1196 1461 DNA Glycine max Predicted polypeptide sequence is orthologousto G1196 1462 DNA Glycine max Predicted polypeptide sequence isorthologous to G1196 1463 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1196 1464 DNA Zea mays Predicted polypeptidesequence is orthologous to G1196 1465 DNA Zea mays Predicted polypeptidesequence is orthologous to G1196 1466 PRT Oryza sativa Orthologous toG1196 1467 PRT Oryza sativa Orthologous to G1196 1468 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1198 1469 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1198 1470 DNAGlycine max Predicted polypeptide sequence is orthologous to G1198 1471DNA Glycine max Predicted polypeptide sequence is orthologous to G11981472 DNA Glycine max Predicted polypeptide sequence is orthologous toG1198 1473 DNA Glycine max Predicted polypeptide sequence is orthologousto G1198 1474 DNA Glycine max Predicted polypeptide sequence isorthologous to G1198 1475 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1198 1476 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1198 1477 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G1198 1478 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G1198 1479 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1198 1480 DNAOryza sativa Predicted polypeptide sequence is orthologous to G1198 1481DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1482DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1483DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1484DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1485DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1486DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1487DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1488DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1489DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1490DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1491DNA Nicotiana tabacum Predicted polypeptide sequence is orthologous toG1198 1492 PRT Oryza sativa Orthologous to G1198 1493 PRT Oryza sativaOrthologous to G1198 1494 PRT Oryza sativa Orthologous to G1198 1495 PRTOryza sativa Orthologous to G1198 1496 PRT Oryza sativa Orthologous toG1198 1497 PRT Oryza sativa Orthologous to G1198 1498 PRT Oryza sativaOrthologous to G1198 1499 DNA Zea mays Predicted polypeptide sequence isorthologous to G1225 1500 PRT Oryza sativa Orthologous to G1225 1501 PRTOryza sativa Orthologous to G1226 1502 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1229 1503 PRT Oryza sativaOrthologous to G1229 1504 PRT Oryza sativa Orthologous to G1229 1505 DNAGlycine max Predicted polypeptide sequence is orthologous to G1255 1506DNA Glycine max Predicted polypeptide sequence is orthologous to G12551507 DNA Glycine max Predicted polypeptide sequence is orthologous toG1255 1508 DNA Glycine max Predicted polypeptide sequence is orthologousto G1255 1509 DNA Glycine max Predicted polypeptide sequence isorthologous to G1255 1510 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1255 1511 DNA Glycine max Predicted polypeptidesequence is orthologous to G1255 1512 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G1255 1513 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G1255 1514 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1255 1515 DNAOryza sativa Predicted polypeptide sequence is orthologous to G1255 1516DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1517DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1518DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1519DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1520DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1521DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1522PRT Oryza sativa Orthologous to G1255 1523 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1266 1524 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1266 1525 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1266 1526 DNAGlycine max Predicted polypeptide sequence is orthologous to G1266 1527DNA Oryza sativa Predicted polypeptide sequence is orthologous to G12661528 PRT Nicotiana tabacum Orthologous to G1266 1529 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G1275 1530 DNA Zea maysPredicted polypeptide sequence is orthologous to G1275 1531 DNA Zea maysPredicted polypeptide sequence is orthologous to G1275 1532 DNA Zea maysPredicted polypeptide sequence is orthologous to G1275 1533 PRT Oryzasativa Orthologous to G1275 1534 PRT Oryza sativa Orthologous to G12751535 PRT Oryza sativa Orthologous to G1275 1536 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1322 1537 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1322 1538 DNAGlycine max Predicted polypeptide sequence is orthologous to G1322 1539DNA Oryza sativa Predicted polypeptide sequence is orthologous to G13221540 PRT Oryza sativa Orthologous to G1322 1541 PRT Oryza sativaOrthologous to G1322 1542 DNA Zea mays Predicted polypeptide sequence isorthologous to G1323 1543 DNA Zea mays Predicted polypeptide sequence isorthologous to G1323 1544 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1330 1545 DNA Glycine max Predicted polypeptidesequence is orthologous to G1330 1546 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1330 1547 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1330 1548 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1330 1549 DNAGlycine max Predicted polypeptide sequence is orthologous to G1330 1550DNA Glycine max Predicted polypeptide sequence is orthologous to G13301551 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG1330 1552 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G1330 1553 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1330 1554 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G1330 1555 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1330 1556 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1330 1557 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1330 1558 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1330 1559 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1330 1560 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1330 1561 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1330 1562 DNA LycopersiconPredicted polypeptide sequence is esculentum orthologous to G1330 1563PRT Oryza sativa Orthologous to G1330 1564 PRT Oryza sativa Orthologousto G1330 1565 PRT Oryza sativa Orthologous to G1330 1566 PRT Oryzasativa Orthologous to G1330 1567 DNA Glycine max Predicted polypeptidesequence is orthologous to G1331 1568 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1331 1569 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G1331 1570 DNA Zea maysPredicted polypeptide sequence is orthologous to G1331 1571 DNA Zea maysPredicted polypeptide sequence is orthologous to G1331 1572 PRT Oryzasativa Orthologous to G1331 1573 DNA Glycine max Predicted polypeptidesequence is orthologous to G1363 1574 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G1363 1575 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G1363 1576 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1363 1577 DNAOryza sativa Predicted polypeptide sequence is orthologous to G1363 1578DNA Zea mays Predicted polypeptide sequence is orthologous to G1363 1579DNA Zea mays Predicted polypeptide sequence is orthologous to G1363 1580DNA Zea mays Predicted polypeptide sequence is orthologous to G1363 1581DNA Zea mays Predicted polypeptide sequence is orthologous to G1363 1582DNA Zea mays Predicted polypeptide sequence is orthologous to G1363 1583PRT Oryza sativa Orthologous to G1363 1584 PRT Oryza sativa Orthologousto G1363 1585 PRT Oryza sativa Orthologous to G1363 1586 PRT Oryzasativa Orthologous to G1363 1587 DNA Glycine max Predicted polypeptidesequence is orthologous to G1411, G2509 1588 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1411, G2509 1589 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1411, G2509 1590 DNAGlycine max Predicted polypeptide sequence is orthologous to G1411,G2509 1591 DNA Zea mays Predicted polypeptide sequence is orthologous toG1411, G2509 1592 DNA Glycine max Predicted polypeptide sequence isorthologous to G1417 1593 PRT Oryza sativa Orthologous to G1417 1594 PRTOryza sativa Orthologous to G1417 1595 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1419 1596 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1449 1597 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1449 1598 DNAOryza sativa Predicted polypeptide sequence is orthologous to G1449 1599DNA Oryza sativa Predicted polypeptide sequence is orthologous to G14491600 DNA Zea mays Predicted polypeptide sequence is orthologous to G14491601 DNA Zea mays Predicted polypeptide sequence is orthologous to G14491602 DNA Zea mays Predicted polypeptide sequence is orthologous to G14491603 DNA Zea mays Predicted polypeptide sequence is orthologous to G14491604 DNA Glycine max Predicted polypeptide sequence is orthologous toG1451 1605 DNA Glycine max Predicted polypeptide sequence is orthologousto G1451 1606 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G1451 1607 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1451 1608 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G1451 1609 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1451 1610 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1451 1611 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1451 1612 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1451 1613 DNA Medicagotruncatula Predicted polypeptide sequence is orthologous to G1451 1614DNA Solanum tuberosum Predicted polypeptide sequence is orthologous toG1451 1615 DNA Zea mays Predicted polypeptide sequence is orthologous toG1451 1616 DNA Sorghum propinquum Predicted polypeptide sequence isorthologous to G1451 1617 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1451 1618 DNA Sorghum bicolor Predicted polypeptidesequence is orthologous to G1451 1619 DNA Hordeum vulgare Predictedpolypeptide sequence is orthologous to G1451 1620 DNA LycopersiconPredicted polypeptide sequence is esculentum orthologous to G1451 1621PRT Oryza sativa Orthologous to G1451 1622 PRT Oryza sativa Orthologousto G1451 1623 PRT Oryza sativa Orthologous to G1451 1624 PRT Oryzasativa Orthologous to G1451 1625 DNA Glycine max Predicted polypeptidesequence is orthologous to G1452 1626 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1478 1627 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1478 1628 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1478 1629 DNA Zeamays Predicted polypeptide sequence is orthologous to G1478 1630 DNAGlycine max Predicted polypeptide sequence is orthologous to G1482 1631DNA Glycine max Predicted polypeptide sequence is orthologous to G14821632 DNA Glycine max Predicted polypeptide sequence is orthologous toG1482 1633 DNA Glycine max Predicted polypeptide sequence is orthologousto G1482 1634 DNA Glycine max Predicted polypeptide sequence isorthologous to G1482 1635 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1482 1636 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G1482 1637 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G1482 1638 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1482 1639 DNAZea mays Predicted polypeptide sequence is orthologous to G1482 1640 DNAZea mays Predicted polypeptide sequence is orthologous to G1482 1641 DNAZea mays Predicted polypeptide sequence is orthologous to G1482 1642 DNAZea mays Predicted polypeptide sequence is orthologous to G1482 1643 DNAZea mays Predicted polypeptide sequence is orthologous to G1482 1644 DNAZea mays Predicted polypeptide sequence is orthologous to G1482 1645 PRTOryza sativa Orthologous to G1482 1646 PRT Oryza sativa Orthologous toG1482 1647 DNA Glycine max Predicted polypeptide sequence is orthologousto G1488 1648 DNA Glycine max Predicted polypeptide sequence isorthologous to G1488 1649 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1488 1650 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1488 1651 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G1488 1652 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1488 1653 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1488 1654 DNA Zea mays Predictedpolypeptide sequence is orthologous to G1488 1655 PRT Oryza sativaOrthologous to G1488 1656 PRT Oryza sativa Orthologous to G1488 1657 PRTOryza sativa Orthologous to G1488 1658 PRT Oryza sativa Orthologous toG1499 1659 DNA Brassica rapa subsp. Predicted polypeptide sequence ispekinensis orthologous to G1499 1660 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1519 1661 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G1519 1662 DNA Zea maysPredicted polypeptide sequence is orthologous to G1519 1663 DNA Zea maysPredicted polypeptide sequence is orthologous to G1519 1664 DNALycopersicon Predicted polypeptide sequence is esculentum orthologous toG1519 1665 DNA Glycine max Predicted polypeptide sequence is orthologousto G1526 1666 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G1526 1667 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1526 1668 DNA Zea mays Predicted polypeptidesequence is orthologous to G1526 1669 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1540 1670 PRT Oryza sativaOrthologous to G1540 1671 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1543 1672 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1543 1673 DNA Zea mays Predicted polypeptidesequence is orthologous to G1543 1674 PRT Oryza sativa Orthologous toG1543 1675 DNA Zea mays Predicted polypeptide sequence is orthologous toG1637 1676 DNA Zea mays Predicted polypeptide sequence is orthologous toG1637 1677 DNA Zea mays Predicted polypeptide sequence is orthologous toG1637 1678 DNA Glycine max Predicted polypeptide sequence is orthologousto G1640 1679 DNA Glycine max Predicted polypeptide sequence isorthologous to G1640 1680 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1640 1681 PRT Oryza sativa Orthologous to G1640 1682DNA Zea mays Predicted polypeptide sequence is orthologous to G1645 1683DNA Zea mays Predicted polypeptide sequence is orthologous to G1645 1684DNA Zea mays Predicted polypeptide sequence is orthologous to G1645 1685DNA Lycopersicon Predicted polypeptide sequence is esculentumorthologous to G1645 1686 DNA Medicago truncatula Predicted polypeptidesequence is orthologous to G1645 1687 PRT Oryza sativa Orthologous toG1645 1688 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G1646 1689 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1646 1690 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1652 1691 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1652 1692 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1652 1693 DNAGlycine max Predicted polypeptide sequence is orthologous to G1652 1694DNA Glycine max Predicted polypeptide sequence is orthologous to G16521695 DNA Glycine max Predicted polypeptide sequence is orthologous toG1652 1696 DNA Glycine max Predicted polypeptide sequence is orthologousto G1652 1697 DNA Glycine max Predicted polypeptide sequence isorthologous to G1652 1698 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1652 1699 DNA Zea mays Predicted polypeptidesequence is orthologous to G1652 1700 DNA Zea mays Predicted polypeptidesequence is orthologous to G1652 1701 PRT Oryza sativa Orthologous toG1652 1702 PRT Oryza sativa Orthologous to G1652 1703 PRT Oryza sativaOrthologous to G1652 1704 PRT Oryza sativa Orthologous to G1652 1705 PRTOryza sativa Orthologous to G1652 1706 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1672 1707 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G1672 1708 DNA Zea maysPredicted polypeptide sequence is orthologous to G1672 1709 DNA Zea maysPredicted polypeptide sequence is orthologous to G1672 1710 PRT Oryzasativa Orthologous to G1672 1711 PRT Oryza sativa Orthologous to G16721712 PRT Oryza sativa Orthologous to G1672 1713 PRT Oryza sativaOrthologous to G1672 1714 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1750 1715 DNA Glycine max Predicted polypeptidesequence is orthologous to G1750 1716 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1750 1717 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1750 1718 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1750 1719 DNAZea mays Predicted polypeptide sequence is orthologous to G1750 1720 DNAZea mays Predicted polypeptide sequence is orthologous to G1750 1721 DNAGlycine max Predicted polypeptide sequence is orthologous to G1756 1722DNA Medicago truncatula Predicted polypeptide sequence is orthologous toG1765 1723 DNA Glycine max Predicted polypeptide sequence is orthologousto G1777 1724 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G1777 1725 DNA Zea mays Predicted polypeptide sequence isorthologous to G1777 1726 DNA Zea mays Predicted polypeptide sequence isorthologous to G1777 1727 PRT Oryza sativa Orthologous to G1777 1728 DNAGlycine max Predicted polypeptide sequence is orthologous to G1792 1729DNA Glycine max Predicted polypeptide sequence is orthologous to G17921730 DNA Glycine max Predicted polypeptide sequence is orthologous toG1792 1731 DNA Glycine max Predicted polypeptide sequence is orthologousto G1792 1732 DNA Glycine max Predicted polypeptide sequence isorthologous to G1792 1733 DNA Zea mays Predicted polypeptide sequence isorthologous to G1792 1734 DNA Lycopersicon Predicted polypeptidesequence is esculentum orthologous to G1792 1735 G3380 PRT Oryza sativaOrthologous to G1792 1736 G3381 PRT Oryza sativa indica Orthologous toG1792 1737 G3383 PRT Oryza sativa japonica Orthologous to G1792 1738 DNAGlycine max Predicted polypeptide sequence is orthologous to G1793 1739DNA Oryza sativa Predicted polypeptide sequence is orthologous to G17931740 DNA Zea mays Predicted polypeptide sequence is orthologous to G17931741 DNA Zea mays Predicted polypeptide sequence is orthologous to G17931742 DNA Zea mays Predicted polypeptide sequence is orthologous to G17931743 PRT Oryza sativa Orthologous to G1793 1744 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1794 1745 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1794 1746 DNAGlycine max Predicted polypeptide sequence is orthologous to G1794 1747DNA Glycine max Predicted polypeptide sequence is orthologous to G17941748 DNA Glycine max Predicted polypeptide sequence is orthologous toG1794 1749 DNA Glycine max Predicted polypeptide sequence is orthologousto G1794 1750 DNA Glycine max Predicted polypeptide sequence isorthologous to G1794 1751 DNA Zea mays Predicted polypeptide sequence isorthologous to G1794 1752 DNA Zea mays Predicted polypeptide sequence isorthologous to G1794 1753 DNA Zea mays Predicted polypeptide sequence isorthologous to G1794 1754 PRT Oryza sativa Orthologous to G1794 1755 PRTOryza sativa Orthologous to G1794 1756 PRT Oryza sativa Orthologous toG1794 1757 DNA Glycine max Predicted polypeptide sequence is orthologousto G1804 1758 DNA Glycine max Predicted polypeptide sequence isorthologous to G1804 1759 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1804 1760 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1804 1761 PRT Oryza sativa Orthologous toG1804 1762 PRT Helianthus annuus Orthologous to G1804 1763 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1838 1764 DNAGlycine max Predicted polypeptide sequence is orthologous to G1838 1765PRT Oryza sativa Orthologous to G1838 1766 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1841 1767 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1841 1768 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1841 1769 PRTOryza sativa Orthologous to G1841 1770 DNA Solanum tuberosum Predictedpolypeptide sequence is orthologous to G1852 1771 DNA Gossypium arboreumPredicted polypeptide sequence is orthologous to G1852 1772 DNA Medicagotruncatula Predicted polypeptide sequence is orthologous to G1852 1773DNA Glycine max Predicted polypeptide sequence is orthologous to G18521774 DNA Lycopersicon Predicted polypeptide sequence is esculentumorthologous to G1852 1775 DNA Pinus taeda Predicted polypeptide sequenceis orthologous to G1852 1776 DNA Lotus japonicus Predicted polypeptidesequence is orthologous to G1852 1777 DNA Gossypium hirsutum Predictedpolypeptide sequence is orthologous to G1852 1778 DNA Solanum tuberosumPredicted polypeptide sequence is orthologous to G1863 1779 DNA Medicagotruncatula Predicted polypeptide sequence is orthologous to G1863 1780DNA Lycopersicon Predicted polypeptide sequence is esculentumorthologous to G1863 1781 PRT Oryza sativa Orthologous to G1863 1782 DNAGlycine max Predicted polypeptide sequence is orthologous to G1880 1783DNA Glycine max Predicted polypeptide sequence is orthologous to G18801784 DNA Medicago truncatula Predicted polypeptide sequence isorthologous to G1880 1785 PRT Oryza sativa Orthologous to G1880 1786 DNAGlycine max Predicted polypeptide sequence is orthologous to G1902 1787DNA Glycine max Predicted polypeptide sequence is orthologous to G19021788 DNA Glycine max Predicted polypeptide sequence is orthologous toG1902 1789 DNA Zea mays Predicted polypeptide sequence is orthologous toG1902 1790 PRT Oryza sativa Orthologous to G1902 1791 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1927 1792 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1927 1793 DNAZea mays Predicted polypeptide sequence is orthologous to G1927 1794 DNALycopersicon Predicted polypeptide sequence is esculentum orthologous toG1927 1795 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G1930 1796 DNA Glycine max Predicted polypeptide sequenceis orthologous to G1944 1797 DNA Glycine max Predicted polypeptidesequence is orthologous to G1944 1798 DNA Zea mays Predicted polypeptidesequence is orthologous to G1944 1799 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1944 1800 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1944 1801 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1946 1802 DNAGlycine max Predicted polypeptide sequence is orthologous to G1946 1803DNA Zea mays Predicted polypeptide sequence is orthologous to G1946 1804DNA Zea mays Predicted polypeptide sequence is orthologous to G1946 1805PRT Oryza sativa Orthologous to G1946 1806 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1948 1807 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1948 1808 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1948 1809 DNAOryza sativa Predicted polypeptide sequence is orthologous to G1948 1810DNA Zea mays Predicted polypeptide sequence is orthologous to G1948 1811DNA Zea mays Predicted polypeptide sequence is orthologous to G1948 1812DNA Zea mays Predicted polypeptide sequence is orthologous to G1948 1813PRT Oryza sativa Orthologous to G1948 1814 DNA Glycine max Predictedpolypeptide sequence is orthologous to G1950 1815 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1950 1816 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1950 1817 DNAGlycine max Predicted polypeptide sequence is orthologous to G1950 1818DNA Glycine max Predicted polypeptide sequence is orthologous to G19501819 DNA Glycine max Predicted polypeptide sequence is orthologous toG1950 1820 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G1950 1821 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1950 1822 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G1950 1823 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G1950 1824 DNA Oryzasativa Predicted polypeptide sequence is orthologous to G1950 1825 DNAOryza sativa Predicted polypeptide sequence is orthologous to G1950 1826DNA Oryza sativa Predicted polypeptide sequence is orthologous to G19501827 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG1950 1828 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G1950 1829 DNA Zea mays Predicted polypeptide sequence isorthologous to G1950 1830 DNA Zea mays Predicted polypeptide sequence isorthologous to G1950 1831 DNA Zea mays Predicted polypeptide sequence isorthologous to G1950 1832 DNA Zea mays Predicted polypeptide sequence isorthologous to G1950 1833 DNA Zea mays Predicted polypeptide sequence isorthologous to G1950 1834 DNA Zea mays Predicted polypeptide sequence isorthologous to G1950 1835 DNA Zea mays Predicted polypeptide sequence isorthologous to G1950 1836 DNA Zea mays Predicted polypeptide sequence isorthologous to G1950 1837 DNA Zea mays Predicted polypeptide sequence isorthologous to G1950 1838 PRT Oryza sativa Orthologous to G1950 1839 PRTOryza sativa Orthologous to G1950 1840 PRT Oryza sativa Orthologous toG1950 1841 PRT Oryza sativa Orthologous to G1950 1842 PRT Oryza sativaOrthologous to G1950 1843 PRT Oryza sativa Orthologous to G1950 1844 PRTOryza sativa Orthologous to G1950 1845 PRT Oryza sativa Orthologous toG1950 1846 PRT Oryza sativa Orthologous to G1950 1847 DNA Glycine maxPredicted polypeptide sequence is orthologous to G1958 1848 DNA Glycinemax Predicted polypeptide sequence is orthologous to G1958 1849 DNAGlycine max Predicted polypeptide sequence is orthologous to G1958 1850DNA Glycine max Predicted polypeptide sequence is orthologous to G19581851 DNA Glycine max Predicted polypeptide sequence is orthologous toG1958 1852 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G1958 1853 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G1958 1854 DNA Zea mays Predicted polypeptidesequence is orthologous to G1958 1855 DNA Zea mays Predicted polypeptidesequence is orthologous to G1958 1856 DNA Zea mays Predicted polypeptidesequence is orthologous to G1958 1857 PRT Nicotiana tabacum Orthologousto G1958 1858 DNA Glycine max Predicted polypeptide sequence isorthologous to G2007 1859 DNA Glycine max Predicted polypeptide sequenceis orthologous to G2007 1860 DNA Zea mays Predicted polypeptide sequenceis orthologous to G2007 1861 DNA Zea mays Predicted polypeptide sequenceis orthologous to G2007 1862 DNA Zea mays Predicted polypeptide sequenceis orthologous to G2007 1863 PRT Oryza sativa Orthologous to G2007 1864DNA Glycine max Predicted polypeptide sequence is orthologous to G2010,G2347 1865 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G2010, G2347 1866 DNA Zea mays Predicted polypeptidesequence is orthologous to G2010 1867 DNA Zea mays Predicted polypeptidesequence is orthologous to G2010, G2347 1868 DNA Glycine max Predictedpolypeptide sequence is orthologous to G2059 1869 DNA Glycine maxPredicted polypeptide sequence is orthologous to G2085 1870 DNA Glycinemax Predicted polypeptide sequence is orthologous to G2085 1871 DNAGlycine max Predicted polypeptide sequence is orthologous to G2085 1872DNA Glycine max Predicted polypeptide sequence is orthologous to G20851873 DNA Zea mays Predicted polypeptide sequence is orthologous to G20851874 PRT Oryza sativa Orthologous to G2085 1875 PRT Oryza sativaOrthologous to G2105 1876 DNA Glycine max Predicted polypeptide sequenceis orthologous to G2110 1877 DNA Oryza sativa Predicted polypeptidesequence is orthologous to G2114 1878 DNA Oryza sativa Predictedpolypeptide sequence is orthologous to G2114 1879 DNA Zea mays Predictedpolypeptide sequence is orthologous to G2114 1880 DNA Zea mays Predictedpolypeptide sequence is orthologous to G2114 1881 DNA Oryza sativaPredicted polypeptide sequence is orthologous to G2117 1882 DNA Medicagotruncatula Predicted polypeptide sequence is orthologous to G2130 1883PRT Oryza sativa Orthologous to G2130 1884 PRT Oryza sativa Orthologousto G2130 1885 DNA Glycine max Predicted polypeptide sequence isorthologous to G2140 1886 DNA Glycine max Predicted polypeptide sequenceis orthologous to G2140 1887 DNA Glycine max Predicted polypeptidesequence is orthologous to G2140 1888 DNA Glycine max Predictedpolypeptide sequence is orthologous to G2140 1889 DNA Glycine maxPredicted polypeptide sequence is orthologous to G2140 1890 DNA Glycinemax Predicted polypeptide sequence is orthologous to G2140 1891 DNAOryza sativa Predicted polypeptide sequence is orthologous to G2140 1892DNA Oryza sativa Predicted polypeptide sequence is orthologous to G21401893 DNA Oryza sativa Predicted polypeptide sequence is orthologous toG2140 1894 DNA Oryza sativa Predicted polypeptide sequence isorthologous to G2140 1895 DNA Zea mays Predicted polypeptide sequence isorthologous to G2140 1896 DNA Lycopersicon Predicted polypeptidesequence is esculentum orthologous to G2140 1897 PRT Oryza sativaOrthologous to G2140 1898 PRT Oryza sativa Orthologous to G2140 1899 PRTOryza sativa Orthologous to G2140 1900 PRT Oryza sativa Orthologous toG2140 1901 PRT Oryza sativa Orthologous to G2140 1902 DNA Glycine maxPredicted polypeptide sequence is orthologous to G2143 1903 DNA Glycinemax Predicted polypeptide sequence is orthologous to G2143 1904 DNAGlycine max Predicted polypeptide sequence is orthologous to G2144 1905DNA Glycine max Predicted polypeptide sequence is orthologous to G21441906 DNA Zea mays Predicted polypeptide sequence is orthologous to G21441907 DNA Zea mays Predicted polypeptide sequence is orthologous to G21441908 DNA Medicago truncatula Predicted polypeptide sequence isorthologous to G2155 1909 DNA Medicago truncatula Predicted polypeptidesequence is orthologous to G2155 1910 DNA Glycine max Predictedpolypeptide sequence is orthologous to G2155 1911 PRT Oryza sativaOrthologous to G2192 1912 PRT Oryza sativa Orthologous to G2295 1913 DNAGlycine max Predicted polypeptide sequence is orthologous to G2340 1914DNA Glycine max Predicted polypeptide sequence is orthologous to G23431915 DNA Glycine max Predicted polypeptide sequence is orthologous toG2343 1916 DNA Glycine max Predicted polypeptide sequence is orthologousto G2343 1917 PRT Lycopersicon Orthologous to G2343 esculentum 1918 PRTOryza sativa Orthologous to G2379 1919 PRT Oryza sativa Orthologous toG2379 1920 PRT Oryza sativa Orthologous to G2379 1921 DNA Glycine maxPredicted polypeptide sequence is orthologous to G2505 1922 DNA Zea maysPredicted polypeptide sequence is orthologous to G2505 1923 DNA Glycinemax Predicted polypeptide sequence is orthologous to G2520 1924 DNAGlycine max Predicted polypeptide sequence is orthologous to G2520 1925DNA Oryza sativa Predicted polypeptide sequence is orthologous to G25201926 DNA Zea mays Predicted polypeptide sequence is orthologous to G25201927 DNA Zea mays Predicted polypeptide sequence is orthologous to G25201928 DNA Zea mays Predicted polypeptide sequence is orthologous to G25201929 PRT Oryza sativa Orthologous to G2520 1930 PRT Oryza sativaOrthologous to G2520 1931 DNA Glycine max Predicted polypeptide sequenceis orthologous to G2557 1932 DNA Glycine max Predicted polypeptidesequence is orthologous to G2557 1933 DNA Glycine max Predictedpolypeptide sequence is orthologous to G2557 1934 DNA Zea mays Predictedpolypeptide sequence is orthologous to G2557 1935 DNA Zea mays Predictedpolypeptide sequence is orthologous to G2557 1936 DNA Glycine maxOrthologous to G2557 1937 PRT Oryza sativa Orthologous to G2557 1938 PRTOryza sativa Orthologous to G2557 1939 PRT Oryza sativa Orthologous toG2557 1940 DNA Glycine max Predicted polypeptide sequence is orthologousto G2719 1941 DNA Zea mays Predicted polypeptide sequence is orthologousto G2719 1942 PRT Oryza sativa Orthologous to G2719 1943 PRT Oryzasativa Orthologous to G2719 1944 DNA Glycine max Predicted polypeptidesequence is orthologous to G2789 1945 DNA Medicago truncatula Predictedpolypeptide sequence is orthologous to G2789 1946 DNA Glycine maxPredicted polypeptide sequence is orthologous to G2830 1947 G5 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG974 1948 G5 PRT Arabidopsis thaliana Paralogous to G974 1949 G9 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG867, G1930 1950 G9 PRT Arabidopsis thaliana Paralogous to G867, G19301951 G12 DNA Arabidopsis thaliana Predicted polypeptide sequence isparalogous to G24 1952 G12 PRT Arabidopsis thaliana Paralogous to G241953 G30 DNA Arabidopsis thaliana Predicted polypeptide sequence isparalogous to G1792 1954 G30 PRT Arabidopsis thaliana Paralogous toG1792 1955 G40 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G912, G913 1956 G40 PRT Arabidopsis thaliana Paralogousto G912, G913 1957 G41 DNA Arabidopsis thaliana Predicted polypeptidesequence is paralogous to G912, G913 1958 G41 PRT Arabidopsis thalianaParalogous to G912, G913 1959 G42 DNA Arabidopsis thaliana Predictedpolypeptide sequence is paralogous to G912, G913 1960 G42 PRTArabidopsis thaliana Paralogous to G912, G913 1961 G182 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G196 1962 G182PRT Arabidopsis thaliana Paralogous to G196 1963 G197 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G664 1964 G197PRT Arabidopsis thaliana Paralogous to G664 1965 G212 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G676 1966 G212PRT Arabidopsis thaliana Paralogous to G676 1967 G216 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G2719 1968 G216PRT Arabidopsis thaliana Paralogous to G2719 1969 G221 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G1322 1970 G221PRT Arabidopsis thaliana Paralogous to G1322 1971 G225 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G226, G682 1972G225 PRT Arabidopsis thaliana Paralogous to G226, G682 1973 G228 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG254 1974 G228 PRT Arabidopsis thaliana Paralogous to G254 1975 G231 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG2007 1976 G231 PRT Arabidopsis thaliana Paralogous to G2007 1977 G233DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG241 1978 G233 PRT Arabidopsis thaliana Paralogous to G241 1979 G247 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG676 1980 G247 PRT Arabidopsis thaliana Paralogous to G676 1981 G249 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG1322 1982 G249 PRT Arabidopsis thaliana Paralogous to G1322 1983 G255DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG664 1984 G255 PRT Arabidopsis thaliana Paralogous to G664 1985 G342 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG343 1986 G342 PRT Arabidopsis thaliana Paralogous to G343 1987 G350 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG545 1988 G350 PRT Arabidopsis thaliana Paralogous to G545 1989 G351 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG545 1990 G351 PRT Arabidopsis thaliana Paralogous to G545 1991 G370 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG361, G362 1992 G370 PRT Arabidopsis thaliana Paralogous to G361, G3621993 G392 DNA Arabidopsis thaliana Predicted polypeptide sequence isparalogous to G390, G391, G438 1994 G392 PRT Arabidopsis thalianaParalogous to G390, G391, G438 1995 G425 DNA Arabidopsis thalianaPredicted polypeptide sequence is paralogous to G427 1996 G425 PRTArabidopsis thaliana Paralogous to G427 1997 G426 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G427 1998 G426PRT Arabidopsis thaliana Paralogous to G427 1999 G440 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G1750 2000 G440PRT Arabidopsis thaliana Paralogous to G1750 2001 G448 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G450 2002 G448PRT Arabidopsis thaliana Paralogous to G450 2003 G455 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G450 2004 G455PRT Arabidopsis thaliana Paralogous to G450 2005 G456 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G450 2006 G456PRT Arabidopsis thaliana Paralogous to G450 2007 G463 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G464 2008 G463PRT Arabidopsis thaliana Paralogous to G464 2009 G485 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G481, G482 2010G485 PRT Arabidopsis thaliana Paralogous to G481, G482 2011 G501 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG519 2012 G501 PRT Arabidopsis thaliana Paralogous to G519 2013 G502 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG519 2014 G502 PRT Arabidopsis thaliana Paralogous to G519 2015 G512 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG1452 2016 G512 PRT Arabidopsis thaliana Paralogous to G1452 2017 G515DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG2053 2018 G515 PRT Arabidopsis thaliana Paralogous to G2053 2019 G516DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG2053 2020 G516 PRT Arabidopsis thaliana Paralogous to G2053 2021 G517DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG2053 2022 G517 PRT Arabidopsis thaliana Paralogous to G2053 2023 G554DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1198 2024 G554 PRT Arabidopsis thaliana Paralogous to G1198 2025 G555DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1198 2026 G555 PRT Arabidopsis thaliana Paralogous to G1198 2027 G556DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1198 2028 G556 PRT Arabidopsis thaliana Paralogous to G1198 2029 G558DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1198 2030 G558 PRT Arabidopsis thaliana Paralogous to G1198 2031 G578DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1198 2032 G578 PRT Arabidopsis thaliana Paralogous to G1198 2033 G580DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG568 2034 G580 PRT Arabidopsis thaliana Paralogous to G568 2035 G586 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG585 2036 G586 PRT Arabidopsis thaliana Paralogous to G585 2037 G596 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG2789 2038 G596 PRT Arabidopsis thaliana Paralogous to G2789 2039 G605DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1944 2040 G605 PRT Arabidopsis thaliana Paralogous to G1944 2041 G610DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG849 2042 G610 PRT Arabidopsis thaliana Paralogous to G849 2043 G629 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG1198 2044 G629 PRT Arabidopsis thaliana Paralogous to G1198 2045 G659DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1323 2046 G659 PRT Arabidopsis thaliana Paralogous to G1323 2047 G666DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG256 2048 G666 PRT Arabidopsis thaliana Paralogous to G256 2049 G668 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG256 2050 G668 PRT Arabidopsis thaliana Paralogous to G256 2051 G671 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG2340 2052 G671 PRT Arabidopsis thaliana Paralogous to G2340 2053 G714DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG489 2054 G714 PRT Arabidopsis thaliana Paralogous to G489 2055 G729 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG1040 2056 G729 PRT Arabidopsis thaliana Paralogous to G1040 2057 G730DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1040 2058 G730 PRT Arabidopsis thaliana Paralogous to G1040 2059 G767DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG519 2060 G767 PRT Arabidopsis thaliana Paralogous to G519 2061 G839 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG1196 2062 G839 PRT Arabidopsis thaliana Paralogous to G1196 2063 G861DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1140 2064 G861 PRT Arabidopsis thaliana Paralogous to G1140 2065 G932DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG256 2066 G932 PRT Arabidopsis thaliana Paralogous to G256 2067 G986 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG881 2068 G986 PRT Arabidopsis thaliana Paralogous to G881 2069 G990 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG1451 2070 G990 PRT Arabidopsis thaliana Paralogous to G1451 2071 G993DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG867, G1930 2072 G993 PRT Arabidopsis thaliana Paralogous to G867, G19302073 G1006 DNA Arabidopsis thaliana Predicted polypeptide sequence isparalogous to G28 2074 G1006 PRT Arabidopsis thaliana Paralogous to G282075 G1008 DNA Arabidopsis thaliana Predicted polypeptide sequence isparalogous to G2130 2076 G1008 PRT Arabidopsis thaliana Paralogous toG2130 2077 G1067 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1073 2078 G1067 PRT Arabidopsis thaliana Paralogous toG1073 2079 G1076 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1075 2080 G1076 PRT Arabidopsis thaliana Paralogous toG1075 2081 G1136 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G584 2082 G1136 PRT Arabidopsis thaliana Paralogous toG584 2083 G1149 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1146 2084 G1149 PRT Arabidopsis thaliana Paralogous toG1146 2085 G1152 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1146 2086 G1152 PRT Arabidopsis thaliana Paralogous toG1146 2087 G1211 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G291 2088 G1211 PRT Arabidopsis thaliana Paralogous toG291 2089 G1277 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G24 2090 G1277 PRT Arabidopsis thaliana Paralogous toG24 2091 G1290 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G278 2092 G1290 PRT Arabidopsis thaliana Paralogous toG278 2093 G1329 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G663 2094 G1329 PRT Arabidopsis thaliana Paralogous toG663 2095 G1335 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G652 2096 G1335 PRT Arabidopsis thaliana Paralogous toG652 2097 G1349 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G896 2098 G1349 PRT Arabidopsis thaliana Paralogous toG896 2099 G1357 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1452 2100 G1357 PRT Arabidopsis thaliana Paralogous toG1452 2101 G1364 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G481, G482 2102 G1364 PRT Arabidopsis thalianaParalogous to G481, G482 2103 G1379 DNA Arabidopsis thaliana Predictedpolypeptide sequence is paralogous to G24 2104 G1379 PRT Arabidopsisthaliana Paralogous to G24 2105 G1387 DNA Arabidopsis thaliana Predictedpolypeptide sequence is paralogous to G975, G2583 2106 G1387 PRTArabidopsis thaliana Paralogous to G975, G2583 2107 G1425 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG504 2108 G1425 PRT Arabidopsis thaliana Paralogous to G504 2109 G1454DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG504 2110 G1454 PRT Arabidopsis thaliana Paralogous to G504 2111 G1456DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1927 2112 G1456 PRT Arabidopsis thaliana Paralogous to G1927 2113 G1461DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1463 2114 G1461 PRT Arabidopsis thaliana Paralogous to G1463 2115 G1462DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1463 2116 G1462 PRT Arabidopsis thaliana Paralogous to G1463 2117 G1464DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1463 2118 G1464 PRT Arabidopsis thaliana Paralogous to G1463 2119 G1465DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1463 2120 G1465 PRT Arabidopsis thaliana Paralogous to G1463 2121 G1484DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG1255 2122 G1484 PRT Arabidopsis thaliana Paralogous to G1255 2123 G1548DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG390, G391, G438 2124 G1548 PRT Arabidopsis thaliana Paralogous to G390,G391, G438 2125 G1646 DNA Arabidopsis thaliana Predicted polypeptidesequence is paralogous to G715 2126 G1646 PRT Arabidopsis thalianaParalogous to G715 2127 G1664 DNA Arabidopsis thaliana Predictedpolypeptide sequence is paralogous to G1062 2128 G1664 PRT Arabidopsisthaliana Paralogous to G1062 2129 G1759 DNA Arabidopsis thalianaPredicted polypeptide sequence is paralogous to G157, G859, G1842, G18432130 G1759 PRT Arabidopsis thaliana Paralogous to G157, G859, G1842,G1843 2131 G1782 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1363 2132 G1782 PRT Arabidopsis thaliana Paralogous toG1363 2133 G1791 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1792 2134 G1791 PRT Arabidopsis thaliana Paralogous toG1792 2135 G1795 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1792 2136 G1795 PRT Arabidopsis thaliana Paralogous toG1792 2137 G1806 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1198 2138 G1806 PRT Arabidopsis thaliana Paralogous toG1198 2139 G1808 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1047 2140 G1808 PRT Arabidopsis thaliana Paralogous toG1047 2141 G1816 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G226, G682 2142 G1816 PRT Arabidopsis thalianaParalogous to G226, G682 2143 G1839 DNA Arabidopsis thaliana Predictedpolypeptide sequence is paralogous to G1749 2144 G1839 PRT Arabidopsisthaliana Paralogous to G1749 2145 G1844 DNA Arabidopsis thalianaPredicted polypeptide sequence is paralogous to G157, G859, G1842, G18432146 G1844 PRT Arabidopsis thaliana Paralogous to G157, G859, G1842,G1843 2147 G1888 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1482 2148 G1888 PRT Arabidopsis thaliana Paralogous toG1482 2149 G1889 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G353, G354 2150 G1889 PRT Arabidopsis thalianaParalogous to G353, G354 2151 G1929 DNA Arabidopsis thaliana Predictedpolypeptide sequence is paralogous to G1478 2152 G1929 PRT Arabidopsisthaliana Paralogous to G1478 2153 G1945 DNA Arabidopsis thalianaPredicted polypeptide sequence is paralogous to G2155 2154 G1945 PRTArabidopsis thaliana Paralogous to G2155 2155 G1974 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G353, G354 2156G1974 PRT Arabidopsis thaliana Paralogous to G353, G354 2157 G1995 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG361, G362 2158 G1995 PRT Arabidopsis thaliana Paralogous to G361, G3622159 G1998 DNA Arabidopsis thaliana Predicted polypeptide sequence isparalogous to G325 2160 G1998 PRT Arabidopsis thaliana Paralogous toG325 2161 G2107 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G912, G913 2162 G2107 PRT Arabidopsis thalianaParalogous to G912, G913 2163 G2131 DNA Arabidopsis thaliana Predictedpolypeptide sequence is paralogous to G979 2164 G2131 PRT Arabidopsisthaliana Paralogous to G979 2165 G2156 DNA Arabidopsis thalianaPredicted polypeptide sequence is paralogous to G1073 2166 G2156 PRTArabidopsis thaliana Paralogous to G1073 2167 G2184 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G1927 2168G2184 PRT Arabidopsis thaliana Paralogous to G1927 2169 G2334 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG1863 2170 G2334 PRT Arabidopsis thaliana Paralogous to G1863 2171 G2345DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG481, G482 2172 G2345 PRT Arabidopsis thaliana Paralogous to G481, G4822173 G2421 DNA Arabidopsis thaliana Predicted polypeptide sequence isparalogous to G663 2174 G2421 PRT Arabidopsis thaliana Paralogous toG663 2175 G2422 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G663 2176 G2422 PRT Arabidopsis thaliana Paralogous toG663 2177 G2423 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1330 2178 G2423 PRT Arabidopsis thaliana Paralogous toG1330 2179 G2424 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G1645 2180 G2424 PRT Arabidopsis thaliana Paralogous toG1645 2181 G2432 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G736 2182 G2432 PRT Arabidopsis thaliana Paralogous toG736 2183 G2513 DNA Arabidopsis thaliana Predicted polypeptide sequenceis paralogous to G912, G913 2184 G2513 PRT Arabidopsis thalianaParalogous to G912, G913 2185 G2535 DNA Arabidopsis thaliana Predictedpolypeptide sequence is paralogous to G961 2186 G2535 PRT Arabidopsisthaliana Paralogous to G961 2187 G2545 DNA Arabidopsis thalianaPredicted polypeptide sequence is paralogous to G427 2188 G2545 PRTArabidopsis thaliana Paralogous to G427 2189 G2631 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G484 2190 G2631PRT Arabidopsis thaliana Paralogous to G484 2191 G2718 DNA Arabidopsisthaliana Predicted polypeptide sequence is paralogous to G226, G682 2192G2718 PRT Arabidopsis thaliana Paralogous to G226, G682 2193 G2776 DNAArabidopsis thaliana Predicted polypeptide sequence is paralogous toG1652 2194 G2776 PRT Arabidopsis thaliana Paralogous to G1652 2195 G2826DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous toG361, G362 2196 G2826 PRT Arabidopsis thaliana Paralogous to G361, G3622197 G2838 DNA Arabidopsis thaliana Predicted polypeptide sequence isparalogous to G361, G362 2198 G2838 PRT Arabidopsis thaliana Paralogousto G361, G362 2199 G2839 DNA Arabidopsis thaliana Predicted polypeptidesequence is paralogous to G353, G354 2200 G2839 PRT Arabidopsis thalianaParalogous to G353, G354 2201 G3010 DNA Arabidopsis thaliana Predictedpolypeptide sequence is paralogous to G987 2202 G3010 PRT Arabidopsisthaliana Paralogous to G987Molecular Modeling

Another means that may be used to confirm the utility and function oftranscription factor sequences that are orthologous or paralogous topresently disclosed transcription factors is through the use ofmolecular modeling software. Molecular modeling is routinely used topredict polypeptide structure, and a variety of protein structuremodeling programs, such as “Insight II” (Accelrys, Inc.) arecommercially available for this purpose. Modeling can thus be used topredict which residues of a polypeptide can be changed without alteringfunction (Crameri et al. (2003) U.S. Pat. No. 6,521,453). Thus,polypeptides that are sequentially similar can be shown to have a highlikelihood of similar function by their structural similarity, whichmay, for example, be established by comparison of regions ofsuperstructure. The relative tendencies of amino acids to form regionsof superstructure (for example, helixes and β-sheets) are wellestablished. For example, O'Neil et al. (1990) Science 250: 646-651)have discussed in detail the helix forming tendencies of amino acids.Tables of relative structure forming activity for amino acids can beused as substitution tables to predict which residues can befunctionally substituted in a given region, for example, in DNA-bindingdomains of known transcription factors and equivalogs. Homologs that arelikely to be functionally similar can then be identified.

Of particular interest is the structure of a transcription factor in theregion of its conserved domain, such as those identified in Table 5.Structural analyses may be performed by comparing the structure of theknown transcription factor around its conserved domain with those oforthologs and paralogs. Analysis of a number of polypeptides within atranscription factor group or clade, including the functionally orsequentially similar polypeptides provided in the Sequence Listing, mayalso provide an understanding of structural elements required toregulate transcription within a given family.

EXAMPLES

The invention, now being generally described, will be more readilyunderstood by reference to the following examples, which are includedmerely for purposes of illustration of certain aspects and embodimentsof the present invention and are not intended to limit the invention. Itwill be recognized by one of skill in the art that a transcriptionfactor that is associated with a particular first trait may also beassociated with at least one other, unrelated and inherent second traitwhich was not predicted by the first trait.

The complete descriptions of the traits associated with eachpolynucleotide of the invention are fully disclosed in Table 4 and Table6. The complete description of the transcription factor gene family andidentified conserved domains of the polypeptide encoded by thepolynucleotide is fully disclosed in Table 5.

Example I Full Length Gene Identification and Cloning

Putative transcription factor sequences (genomic or ESTs) related toknown transcription factors were identified in the Arabidopsis thalianaGenBank database using the tblastn sequence analysis program usingdefault parameters and a P-value cutoff threshold of −4 or −5 or lower,depending on the length of the query sequence. Putative transcriptionfactor sequence hits were then screened to identify those containingparticular sequence strings. If the sequence hits contained suchsequence strings, the sequences were confirmed as transcription factors.

Alternatively, Arabidopsis thaliana cDNA libraries derived fromdifferent tissues or treatments, or genomic libraries were screened toidentify novel members of a transcription family using a low stringencyhybridization approach. Probes were synthesized using gene specificprimers in a standard PCR reaction (annealing temperature 60° C.) andlabeled with ³²P dCTP using the High Prime DNA Labeling Kit (BoehringerMannheim Corp. (now Roche Diagnostics Corp., Indianapolis, Ind.).Purified radiolabelled probes were added to filters immersed in Churchhybridization medium (0.5 M NaPO₄ pH 7.0, 7% SDS, 1% w/v bovine serumalbumin) and hybridized overnight at 60° C. with shaking. Filters werewashed two times for 45 to 60 minutes with 1×SCC, 1% SDS at 60° C.

To identify additional sequence 5′ or 3′ of a partial cDNA sequence in acDNA library, 5′ and 3′ rapid amplification of cDNA ends (RACE) wasperformed using the MARATHON cDNA amplification kit (Clontech, PaloAlto, Calif.). Generally, the method entailed first isolating poly(A)mRNA, performing first and second strand cDNA synthesis to generatedouble stranded cDNA, blunting cDNA ends, followed by ligation of theMARATHON Adaptor to the cDNA to form a library of adaptor-ligated dscDNA.

Gene-specific primers were designed to be used along with adaptorspecific primers for both 5′ and 3′ RACE reactions. Nested primers,rather than single primers, were used to increase PCR specificity. Using5′ and 3′ RACE reactions, 5′ and 3′ RACE fragments were obtained,sequenced and cloned. The process can be repeated until 5′ and 3′ endsof the full-length gene were identified. Then the full-length cDNA wasgenerated by PCR using primers specific to 5′ and 3′ ends of the gene byend-to-end PCR.

Example II Construction of Expression Vectors

The sequence was amplified from a genomic or cDNA library using primersspecific to sequences upstream and downstream of the coding region. Theexpression vector was pMEN20 or pMEN65, which are both derived frompMON316 (Sanders et al. (1987) Nucleic Acids Res. 15:1543-1558) andcontain the CaMV 35S promoter to express transgenes. To clone thesequence into the vector, both pMEN20 and the amplified DNA fragmentwere digested separately with SalI and Nod restriction enzymes at 37° C.for 2 hours. The digestion products were subject to electrophoresis in a0.8% agarose gel and visualized by ethidium bromide staining. The DNAfragments containing the sequence and the linearized plasmid wereexcised and purified by using a QIAQUICK gel extraction kit (Qiagen,Valencia Calif.). The fragments of interest were ligated at a ratio of3:1 (vector to insert). Ligation reactions using T4 DNA ligase (NewEngland Biolabs, Beverly Mass.) were carried out at 16° C. for 16 hours.The ligated DNAs were transformed into competent cells of the E. colistrain DH5alpha by using the heat shock method. The transformations wereplated on LB plates containing 50 mg/l kanamycin (Sigma Chemical Co. St.Louis Mo.). Individual colonies were grown overnight in five millilitersof LB broth containing 50 mg/l kanamycin at 37° C. Plasmid DNA waspurified by using Qiaquick Mini Prep kits (Qiagen).

Example III Transformation of Agrobacterium with the Expression Vector

After the plasmid vector containing the gene was constructed, the vectorwas used to transform Agrobacterium tumefaciens cells expressing thegene products. The stock of Agrobacterium tumefaciens cells fortransformation were made as described by Nagel et al. (1990) FEMSMicrobiol Letts. 67: 325-328. Agrobacterium strain ABI was grown in 250ml LB medium (Sigma) overnight at 28° C. with shaking until anabsorbance over 1 cm at 600 nm (A₆₀₀) of 0.5-1.0 was reached. Cells wereharvested by centrifugation at 4,000×g for 15 min at 4° C. Cells werethen resuspended in 250 μl chilled buffer (1 mM HEPES, pH adjusted to7.0 with KOH). Cells were centrifuged again as described above andresuspended in 125 μl chilled buffer. Cells were then centrifuged andresuspended two more times in the same HEPES buffer as described aboveat a volume of 100 μl and 750 μl, respectively. Resuspended cells werethen distributed into 40 μl aliquots, quickly frozen in liquid nitrogen,and stored at −80° C.

Agrobacterium cells were transformed with plasmids prepared as describedabove following the protocol described by Nagel et al. (supra). For eachDNA construct to be transformed, 50-100 ng DNA (generally resuspended in10 mM Tris-HCl, 1 mM EDTA, pH 8.0) was mixed with 40 μl of Agrobacteriumcells. The DNA/cell mixture was then transferred to a chilled cuvettewith a 2 mm electrode gap and subject to a 2.5 kV charge dissipated at25° F. and 200° F. using a Gene Pulser II apparatus (Bio-Rad, Hercules,Calif.). After electroporation, cells were immediately resuspended in1.0 ml LB and allowed to recover without antibiotic selection for 2-4hours at 28° C. in a shaking incubator. After recovery, cells wereplated onto selective medium of LB broth containing 100 μg/mlspectinomycin (Sigma) and incubated for 24-48 hours at 28° C. Singlecolonies were then picked and inoculated in fresh medium. The presenceof the plasmid construct was verified by PCR amplification and sequenceanalysis.

Example IV Transformation of Arabidopsis Plants with Agrobacteriumtumefaciens with Expression Vector

After transformation of Agrobacterium tumefaciens with plasmid vectorscontaining the gene, single Agrobacterium colonies were identified,propagated, and used to transform Arabidopsis plants. Briefly, 500 mlcultures of LB medium containing 50 mg/l kanamycin were inoculated withthe colonies and grown at 28° C. with shaking for 2 days until anoptical absorbance at 600 nm wavelength over 1 cm (A₆₀₀) of >2.0 isreached. Cells were then harvested by centrifugation at 4,000×g for 10min, and resuspended in infiltration medium (½× Murashige and Skoogsalts (Sigma), 1× Gamborg's B-5 vitamins (Sigma), 5.0% (w/v) sucrose(Sigma), 0.044 μM benzylamino purine (Sigma), 200 μl/l Silwet L-77(Lehle Seeds) until an A₆₀₀ of 0.8 was reached.

Prior to transformation, Arabidopsis thaliana seeds (ecotype Columbia)were sown at a density of ˜10 plants per 4″ pot onto Pro-Mix BX pottingmedium (Hummert International) covered with fiberglass mesh (18 mm×16mm) Plants were grown under continuous illumination (50-75 μE/m²/sec) at22-23° C. with 65-70% relative humidity. After about 4 weeks, primaryinflorescence stems (bolts) are cut off to encourage growth of multiplesecondary bolts. After flowering of the mature secondary bolts, plantswere prepared for transformation by removal of all siliques and openedflowers.

The pots were then immersed upside down in the mixture of Agrobacteriuminfiltration medium as described above for 30 sec, and placed on theirsides to allow draining into a 1′×2′ flat surface covered with plasticwrap. After 24 h, the plastic wrap was removed and pots are turnedupright. The immersion procedure was repeated one week later, for atotal of two immersions per pot. Seeds were then collected from eachtransformation pot and analyzed following the protocol described below.

Example V Identification of Arabidopsis Primary Transformants

Seeds collected from the transformation pots were sterilized essentiallyas follows. Seeds were dispersed into in a solution containing 0.1%(v/v) Triton X-100 (Sigma) and sterile water and washed by shaking thesuspension for 20 min. The wash solution was then drained and replacedwith fresh wash solution to wash the seeds for 20 min with shaking Afterremoval of the ethanol/detergent solution, a solution containing 0.1%(v/v) Triton X-100 and 30% (v/v) bleach (CLOROX; Clorox Corp. OaklandCalif.) was added to the seeds, and the suspension was shaken for 10min. After removal of the bleach/detergent solution, seeds were thenwashed five times in sterile distilled water. The seeds were stored inthe last wash water at 4° C. for 2 days in the dark before being platedonto antibiotic selection medium (1× Murashige and Skoog salts (pHadjusted to 5.7 with 1M KOH), 1× Gamborg's B-5 vitamins, 0.9% phytagar(Life Technologies), and 50 mg/l kanamycin). Seeds were germinated undercontinuous illumination (50-75 μE/m²/sec) at 22-23° C. After 7-10 daysof growth under these conditions, kanamycin resistant primarytransformants (T₁ generation) were visible and obtained. These seedlingswere transferred first to fresh selection plates where the seedlingscontinued to grow for 3-5 more days, and then to soil (Pro-Mix BXpotting medium).

Primary transformants were crossed and progeny seeds (T₂) collected;kanamycin resistant seedlings were selected and analyzed. The expressionlevels of the recombinant polynucleotides in the transformants variesfrom about a 5% expression level increase to a least a 100% expressionlevel increase. Similar observations are made with respect topolypeptide level expression.

Example VI Identification of Arabidopsis Plants with TranscriptionFactor Gene Knockouts

The screening of insertion mutagenized Arabidopsis collections for nullmutants in a known target gene was essentially as described in Krysan etal. (1999) Plant Cell 11: 2283-2290. Briefly, gene-specific primers,nested by 5-250 base pairs to each other, were designed from the 5′ and3′ regions of a known target gene. Similarly, nested sets of primerswere also created specific to each of the T-DNA or transposon ends (the“right” and “left” borders). All possible combinations of gene specificand T-DNA/transposon primers were used to detect by PCR an insertionevent within or close to the target gene. The amplified DNA fragmentswere then sequenced which allows the precise determination of theT-DNA/transposon insertion point relative to the target gene. Insertionevents within the coding or intervening sequence of the genes weredeconvoluted from a pool comprising a plurality of insertion events to asingle unique mutant plant for functional characterization. The methodis described in more detail in Yu and Adam, U.S. application Ser. No.09/177,733 filed Oct. 23, 1998.

Example VII Identification of Modified Phenotypes in Overexpression orGene Knockout Plants

Experiments were performed to identify those transformants or knockoutsthat exhibited modified biochemical characteristics. Among thebiochemicals that were assayed were insoluble sugars, such as arabinose,fucose, galactose, mannose, rhamnose or xylose or the like; prenyllipids, such as lutein, beta-carotene, xanthophyll-1, xanthophyll-2,chlorophylls A or B, or alpha-, delta- or gamma-tocopherol or the like;fatty acids, such as 16:0 (palmitic acid), 16:1 (palmitoleic acid), 18:0(stearic acid), 18:1 (oleic acid), 18:2 (linoleic acid), 20:0, 18:3(linolenic acid), 20:1 (eicosenoic acid), 20:2, 22:1 (erucic acid) orthe like; waxes, such as by altering the levels of C29, C31, or C33alkanes; sterols, such as brassicasterol, campesterol, stigmasterol,sitosterol or stigmastanol or the like, glucosinolates, protein or oillevels.

Fatty acids were measured using two methods depending on whether thetissue was from leaves or seeds. For leaves, lipids were extracted andesterified with hot methanolic H₂SO₄ and partitioned into hexane frommethanolic brine. For seed fatty acids, seeds were pulverized andextracted in methanol:heptane:toluene:2,2-dimethoxypropane:H₂SO₄(39:34:20:5:2) for 90 minutes at 80° C. After cooling to roomtemperature the upper phase, containing the seed fatty acid esters, wassubjected to GC analysis. Fatty acid esters from both seed and leaftissues were analyzed with a SUPELCO SP-2330 column (Supelco,Bellefonte, Pa.).

Glucosinolates were purified from seeds or leaves by first heating thetissue at 95° C. for 10 minutes. Preheated ethanol:water (50:50) isadded and after heating at 95° C. for a further 10 minutes, theextraction solvent is applied to a DEAE Sephadex column (Pharmacia)which had been previously equilibrated with 0.5 M pyridine acetate.Desulfoglucosinolates were eluted with 300 ul water and analyzed byreverse phase HPLC monitoring at 226 nm.

For wax alkanes, samples were extracted using an identical method asfatty acids and extracts were analyzed on a HP 5890 GC coupled with a5973 MSD. Samples were chromatographically isolated on a J&W DB35 massspectrometer (J&W Scientific Agilent Technologies, Folsom, Calif.).

To measure prenyl lipid levels, seeds or leaves were pulverized with 1to 2% pyrogallol as an antioxidant. For seeds, extracted samples werefiltered and a portion removed for tocopherol and carotenoid/chlorophyllanalysis by HPLC. The remaining material was saponified for steroldetermination. For leaves, an aliquot was removed and diluted withmethanol and chlorophyll A, chlorophyll B, and total carotenoidsmeasured by spectrophotometry by determining optical absorbance at 665.2nm, 652.5 nm, and 470 nm. An aliquot was removed for tocopherol andcarotenoid/chlorophyll composition by HPLC using a Waters μBondapak C18column (4.6 mm×150 mm). The remaining methanolic solution was saponifiedwith 10% KOH at 80° C. for one hour. The samples were cooled and dilutedwith a mixture of methanol and water. A solution of 2% methylenechloride in hexane was mixed in and the samples were centrifuged. Theaqueous methanol phase was again re-extracted 2% methylene chloride inhexane and, after centrifugation, the two upper phases were combined andevaporated. 2% methylene chloride in hexane was added to the tubes andthe samples were then extracted with one ml of water. The upper phasewas removed, dried, and resuspended in 400 ul of 2% methylene chloridein hexane and analyzed by gas chromatography using a 50 m DB-5 ms (0.25mm ID, 0.25 um phase, J&W Scientific).

Insoluble sugar levels were measured by the method essentially describedby Reiter et al. (1999), Plant J. 12: 335-345. This method analyzes theneutral sugar composition of cell wall polymers found in Arabidopsisleaves. Soluble sugars were separated from sugar polymers by extractingleaves with hot 70% ethanol. The remaining residue containing theinsoluble polysaccharides was then acid hydrolyzed with allose added asan internal standard. Sugar monomers generated by the hydrolysis werethen reduced to the corresponding alditols by treatment with NaBH4, thenwere acetylated to generate the volatile alditol acetates which werethen analyzed by GC-FID. Identity of the peaks was determined bycomparing the retention times of known sugars converted to thecorresponding alditol acetates with the retention times of peaks fromwild-type plant extracts. Alditol acetates were analyzed on a SupelcoSP-2330 capillary column (30 m×250 μm×0.2 μm) using a temperatureprogram beginning at 180° C. for 2 minutes followed by an increase to220° C. in 4 minutes. After holding at 220° C. for 10 minutes, the oventemperature is increased to 240° C. in 2 minutes and held at thistemperature for 10 minutes and brought back to room temperature.

To identify plants with alterations in total seed oil or proteincontent, 150 mg of seeds from T2 progeny plants were subjected toanalysis by Near Infrared Reflectance Spectroscopy (NIRS) using a FossNirSystems Model 6500 with a spinning cup transport system. NIRS is anon-destructive analytical method used to determine seed oil and proteincomposition. Infrared is the region of the electromagnetic spectrumlocated after the visible region in the direction of longer wavelengths.‘Near infrared’ owns its name for being the infrared region near to thevisible region of the electromagnetic spectrum. For practical purposes,near infrared comprises wavelengths between 800 and 2500 nm. NIRS isapplied to organic compounds rich in O—H bonds (such as moisture,carbohydrates, and fats), C—H bonds (such as organic compounds andpetroleum derivatives), and N—H bonds (such as proteins and aminoacids). The NIRS analytical instruments operate by statisticallycorrelating NIRS signals at several wavelengths with the characteristicor property intended to be measured. All biological substances containthousands of C—H, O—H, and N—H bonds. Therefore, the exposure to nearinfrared radiation of a biological sample, such as a seed, results in acomplex spectrum which contains qualitative and quantitative informationabout the physical and chemical composition of that sample.

The numerical value of a specific analyte in the sample, such as proteincontent or oil content, is mediated by a calibration approach known aschemometrics. Chemometrics applies statistical methods such as multiplelinear regression (MLR), partial least squares (PLS), and principlecomponent analysis (PCA) to the spectral data and correlates them with aphysical property or other factor, that property or factor is directlydetermined rather than the analyte concentration itself. The methodfirst provides “wet chemistry” data of the samples required to developthe calibration.

Calibration of NIRS response was performed using data obtained by wetchemical analysis of a population of Arabidopsis ecotypes that wereexpected to represent diversity of oil and protein levels.

The exact oil composition of each ecotype used in the calibrationexperiment was performed using gravimetric analysis of oils extractedfrom seed samples (0.5 g or 1.0 g) by the accelerated solvent extractionmethod (ASE; Dionex Corp, Sunnyvale, Calif.). The extraction method wasvalidated against certified canola samples (Community Bureau ofReference, Belgium). Seed samples from each ecotype (0.5 g or 1 g) weresubjected to accelerated solvent extraction and the resulting extractedoil weights compared to the weight of oil recovered from canola seedthat has been certified for oil content (Community Bureau of Reference).The oil calibration equation was based on 57 samples with a range of oilcontents from 27.0% to 50.8%. To check the validity of the calibrationcurve, an additional set of samples was extracted by ASE and predictedusing the oil calibration equation. This validation set counted 46samples, ranging from 27.9% to 47.5% oil, and had a predicted standarderror of performance of 0.63%. The wet chemical method for protein waselemental analysis (% N×6.0) using the average of 3 representativesamples of 5 mg each validated against certified ground corn (NIST). Theinstrumentation was an Elementar Vario-EL III elemental analyzeroperated in CNS operating mode (Elementar Analysensysteme GmbH, Hanau,Germany).

The protein calibration equation was based on a library of 63 sampleswith a range of protein contents from 17.4% to 31.2%. An additional setof samples was analyzed for protein by elemental analysis (n=57) andscanned by NIRS in order to validate the protein prediction equation.The protein range of the validation set was from 16.8% to 31.2% and thestandard error of prediction was 0.468%.

NIRS analysis of Arabidopsis seed was carried out on between 40-300 mgexperimental sample. The oil and protein contents were predicted usingthe respective calibration equations.

Data obtained from NIRS analysis was analyzed statistically using anearest-neighbor (N-N) analysis. The N-N analysis allows removal ofwithin-block spatial variability in a fairly flexible fashion, whichdoes not require prior knowledge of the pattern of variability in thechamber. Ideally, all hybrids are grown under identical experimentalconditions within a block (rep). In reality, even in many block designs,significant within-block variability exists. Nearest-neighbor proceduresare based on assumption that environmental effect of a plot is closelyrelated to that of its neighbors. Nearest-neighbor methods useinformation from adjacent plots to adjust for within-block heterogeneityand so provide more precise estimates of treatment means anddifferences. If there is within-plot heterogeneity on a spatial scalethat is larger than a single plot and smaller than the entire block,then yields from adjacent plots will be positively correlated.Information from neighboring plots can be used to reduce or remove theunwanted effect of the spatial heterogeneity, and hence improve theestimate of the treatment effect. Data from neighboring plots can alsobe used to reduce the influence of competition between adjacent plots.The Papadakis N-N analysis can be used with designs to removewithin-block variability that would not be removed with the standardsplit plot analysis (Papadakis (1973) Inst. d'Amelior. PlantesThessaloniki (Greece) Bull. Scientif. No. 23; Papadakis (1984) Proc.Acad. Athens 59: 326-342.

Experiments were performed to identify those transformants or knockoutsthat exhibited modified sugar-sensing. For such studies, seeds fromtransformants were germinated on media containing 5% glucose or 9.4%sucrose which normally partially restrict hypocotyl elongation. Plantswith altered sugar sensing may have either longer or shorter hypocotylsthan normal plants when grown on this media. Additionally, other planttraits may be varied such as root mass.

Experiments may be performed to identify those transformants orknockouts that exhibited an improved pathogen tolerance. For suchstudies, the transformants are exposed to biotropic fungal pathogens,such as Erysiphe orontii, and necrotropic fungal pathogens, such asFusarium oxysporum. Fusarium oxysporum isolates cause vascular wilts anddamping off of various annual vegetables, perennials and weeds(Mauch-Mani and Slusarenko (1994) Molec Plant-Microbe Interact. 7:378-383). For Fusarium oxysporum experiments, plants are grown on Petridishes and sprayed with a fresh spore suspension of F. oxysporum. Thespore suspension is prepared as follows: A plug of fungal hyphae from aplate culture is placed on a fresh potato dextrose agar plate andallowed to spread for one week. Five ml sterile water is then added tothe plate, swirled, and pipetted into 50 ml Armstrong Fusarium medium.Spores are grown overnight in Fusarium medium and then sprayed ontoplants using a Preval paint sprayer. Plant tissue is harvested andfrozen in liquid nitrogen 48 hours post-infection.

Erysiphe orontii is a causal agent of powdery mildew. For Erysipheorontii experiments, plants are grown approximately 4 weeks in agreenhouse under 12 hour light (20° C., ˜30% relative humidity (rh)).Individual leaves are infected with E. orontii spores from infectedplants using a camel's hair brush, and the plants are transferred to aPercival growth chamber (20° C., 80% rh.). Plant tissue is harvested andfrozen in liquid nitrogen 7 days post-infection.

Botrytis cinerea is a necrotrophic pathogen. Botrytis cinerea is grownon potato dextrose agar under 12 hour light (20° C., ˜30% relativehumidity (rh)). A spore culture is made by spreading 10 ml of sterilewater on the fungus plate, swirling and transferring spores to 10 ml ofsterile water. The spore inoculum (approx. 105 spores/ml) is then usedto spray 10 day-old seedlings grown under sterile conditions on MS(minus sucrose) media. Symptoms are evaluated every day up toapproximately 1 week.

Sclerotinia sclerotiorum hyphal cultures are grown in potato dextrosebroth. One gram of hyphae is ground, filtered, spun down and resuspendedin sterile water. A 1:10 dilution is used to spray 10 day-old seedlingsgrown aseptically under a 12 hour light/dark regime on MS (minussucrose) media. Symptoms are evaluated every day up to approximately 1week.

Pseudomonas syringae pv maculicola (Psm) strain 4326 and pv maculicolastrain 4326 was inoculated by hand at two doses. Two inoculation dosesallows the differentiation between plants with enhanced susceptibilityand plants with enhanced resistance to the pathogen. Plants are grownfor 3 weeks in the greenhouse, then transferred to the growth chamberfor the remainder of their growth. Psm ES4326 may be hand inoculatedwith 1 ml syringe on 3 fully-expanded leaves per plant (4½ wk old),using at least 9 plants per overexpressing line at two inoculationdoses, OD=0.005 and OD=0.0005. Disease scoring is performed at day 3post-inoculation with pictures of the plants and leaves taken inparallel.

In some instances, expression patterns of the pathogen-induced genes(such as defense genes) may be monitored by microarray experiments. Inthese experiments, cDNAs are generated by PCR and resuspended at a finalconcentration of ˜100 ng/μl in 3×SSC or 150 mM Na-phosphate (Eisen andBrown (1999) Methods Enzymol. 303: 179-205). The cDNAs are spotted onmicroscope glass slides coated with polylysine. The prepared cDNAs arealiquoted into 384 well plates and spotted on the slides using, forexample, an x-y-z gantry (OmniGrid) which may be purchased fromGeneMachines (Menlo Park, Calif.) outfitted with quill type pins whichmay be purchased from Telechem International (Sunnyvale, Calif.). Afterspotting, the arrays are cured for a minimum of one week at roomtemperature, rehydrated and blocked following the protocol recommendedby Eisen and Brown (1999; supra).

Sample total RNA (10 μg) samples are labeled using fluorescent Cy3 andCy5 dyes. Labeled samples are resuspended in 4×SSC/0.03% SDS/4 μg salmonsperm DNA/2 μg tRNA/50 mM Na-pyrophosphate, heated for 95° C. for 2.5minutes, spun down and placed on the array. The array is then coveredwith a glass coverslip and placed in a sealed chamber. The chamber isthen kept in a water bath at 62° C. overnight. The arrays are washed asdescribed in Eisen and Brown (1999, supra) and scanned on a GeneralScanning 3000 laser scanner. The resulting files are subsequentlyquantified using IMAGENE, software (BioDiscovery, Los Angeles Calif.).

RT-PCR experiments may be performed to identify those genes inducedafter exposure to biotropic fungal pathogens, such as Erysiphe orontii,necrotropic fungal pathogens, such as Fusarium oxysporum, bacteria,viruses and salicylic acid, the latter being involved in a nonspecificresistance response in Arabidopsis thaliana. Generally, the geneexpression patterns from ground plant leaf tissue is examined.

Reverse transcriptase PCR was conducted using gene specific primerswithin the coding region for each sequence identified. The primers weredesigned near the 3′ region of each DNA binding sequence initiallyidentified.

Total RNA from these ground leaf tissues was isolated using the CTABextraction protocol. Once extracted total RNA was normalized inconcentration across all the tissue types to ensure that the PCRreaction for each tissue received the same amount of cDNA template usingthe 28S band as reference. Poly(A+) RNA was purified using a modifiedprotocol from the Qiagen OLIGOTEX purification kit batch protocol. cDNAwas synthesized using standard protocols. After the first strand cDNAsynthesis, primers for Actin 2 were used to normalize the concentrationof cDNA across the tissue types. Actin 2 is found to be constitutivelyexpressed in fairly equal levels across the tissue types beinginvestigated.

For RT PCR, cDNA template was mixed with corresponding primers and TaqDNA polymerase. Each reaction consisted of 0.2 μl cDNA template, 2 μl10× Tricine buffer, 2 μl 10× Tricine buffer and 16.8 μl water, 0.05 μlPrimer 1, 0.05 μl, Primer 2, 0.3 μl Taq DNA polymerase and 8.6 μl water.

The 96 well plate is covered with microfilm and set in the thermocyclerto start the reaction cycle. By way of illustration, the reaction cyclemay comprise the following steps:

Step 1: 93° C. for 3 min;

Step 2: 93° C. for 30 sec;

Step 3: 65° C. for 1 min;

Step 4: 72° C. for 2 min;

Steps 2, 3 and 4 are repeated for 28 cycles;

Step 5: 72° C. for 5 min; and

Step 6: 4° C.

To amplify more products, for example, to identify genes that have verylow expression, additional steps may be performed: The following methodillustrates a method that may be used in this regard. The PCR plate isplaced back in the thermocycler for 8 more cycles of steps 2-4.

Step 2: 93° C. for 30 sec;

Step 3: 65° C. for 1 min;

Step 4: 72° C. for 2 min, repeated for 8 cycles; and

Step 5: 4° C.

Eight microliters of PCR product and 1.5 μl of loading dye are loaded ona 1.2% agarose gel for analysis after 28 cycles and 36 cycles.Expression levels of specific transcripts are considered low if theywere only detectable after 36 cycles of PCR. Expression levels areconsidered medium or high depending on the levels of transcript comparedwith observed transcript levels for an internal control such as actin2.Transcript levels are determined in repeat experiments and compared totranscript levels in control (e.g., non-transformed) plants.

Experiments were performed to identify those transformants or knockoutsthat exhibited an improved environmental stress tolerance. For suchstudies, the transformants were exposed to a variety of environmentalstresses. Plants were exposed to chilling stress (6 hour exposure to4-8° C.), heat stress (6 hour exposure to 32-37° C.), high salt stress(6 hour exposure to 200 mM NaCl), drought stress (168 hours afterremoving water from trays), osmotic stress (6 hour exposure to 3 Mmannitol), or nutrient limitation (nitrogen: all components of MS mediumremained constant except N was reduced to 20 mg/l of NH₄NO₃; phosphate:all components of MS medium except KH2PO₄, which was replaced by K₂SO₄;potassium: all components of MS medium except removal of KNO₃ andKH₂PO₄, which were replaced by NaH₄PO₄).

Experiments were performed to identify those transformants or knockoutsthat exhibited a modified structure and development characteristics. Forsuch studies, the transformants were observed by eye to identify novelstructural or developmental characteristics associated with the ectopicexpression of the polynucleotides or polypeptides of the invention.

Flowering time was measured by the number of rosette leaves present whena visible inflorescence of approximately 3 cm is apparent. Rosette andtotal leaf number on the progeny stem are tightly correlated with thetiming of flowering (Koornneef et al. (1991) Mol. Gen. Genet. 229:57-66). The vernalization response was also measured. For vernalizationtreatments, seeds were sown to MS agar plates, sealed with microporetape, and placed in a 4° C. cold room with low light levels for 6-8weeks. The plates were then transferred to the growth rooms alongsideplates containing freshly sown non-vernalized controls. Rosette leaveswere counted when a visible inflorescence of approximately 3 cm wasapparent.

Modified phenotypes observed for particular overexpressor or knockoutplants are provided in Table 4. For a particular overexpressor thatshows a less beneficial characteristic, it may be more useful to selecta plant with a decreased expression of the particular transcriptionfactor. For a particular knockout that shows a less beneficialcharacteristic, it may be more useful to select a plant with anincreased expression of the particular transcription factor.

The sequences of the Sequence Listing or those in Tables 4-8, or thosedisclosed here, can be used to prepare transgenic plants and plants withaltered traits. The specific transgenic plants listed below are producedfrom the sequences of the Sequence Listing, as noted. Table 4 providesexemplary polynucleotide and polypeptide sequences of the invention.

Example VIII Examples of Genes that Confer Significant Improvements toPlants

A number of genes and homologs that confer significant improvements toknockout or overexpressing plants were noted below. Experimentalobservations made with regard to specific genes whose expression wasmodified in overexpressing or knockout plants, and potentialapplications based on these observations, were also presented.

G8 (SEQ ID NO: 1)

Published Information

G8 corresponds to gene At2g28550 (AAD21489), and was described as RAP2.7(Okamuro et al. (1997) Proc. Natl. Acad. Sci. 94:7076-7081).

Experimental Observations

The function of G8 was studied using transgenic plants in which the genewas expressed under the control of the 35S promoter. Overexpression ofG8 caused alterations in plant development, the most consistent onebeing a delay in flowering time.

The individual plants showed a relatively strong phenotype and typicallymade 30-50 leaves (versus 10-12 the wild-type controls) prior tobolting, under 24-hour light. This phenotype was reproduced in some, butnot all, of the T2 progeny plants from each one of the lines.Additionally, a further T2 population was found to flower later thanwild type in 12-hour light conditions. Thus, late flowering was observedin both the T1 and T2 generations, and in different photoperiodicconditions.

It should also be noted that many 35S::G8 plants appeared smaller thancontrols, particularly at early stages. Accordingly, in the T2 linesused for physiological analyses it was observed that seedlings weresmaller and showed reduced vigor when germinated on MS plates. However,not all 35S::G8 lines showed these effects.

G8 was ubiquitously expressed, at higher levels in rosette leaves.

Potential Applications

G8 or its equivalogs can be used to alter flowering time.

In general, a wide variety of applications exist for systems that eitherlengthen or shorten the time to flowering.

Most modern crop varieties were the result of extensive breedingprograms. Many generations of backcrossing may be required to introducedesired traits. Systems that accelerate flowering can have valuableapplications in such programs since they allow much faster generationtimes. Additionally, in some instances, a faster generation time canallow additional harvests of a crop to be made within a given growingseason. With the advent of transformation systems for tree species suchas oil palm and Eucalyptus, forest biotechnology is a growing area ofinterest. Acceleration of flowering can reduce generation times and makebreeding programs feasible which would otherwise be impossible.

In species such as sugarbeet where the vegetative parts of the plantsconstitute the crop and the reproductive tissues were discarded, it isadvantageous to delay or prevent flowering. Extending vegetativedevelopment can bring about large increases in yields. By regulating theexpression of flowering-time controlling genes, using induciblepromoters, flowering can be triggered as desired (for example, byapplication of a chemical inducer). This can allow, for example,flowering to be synchronized across a crop and facilitate more efficientharvesting. Such inducible systems can be used to tune the flowering ofcrop varieties to different latitudes. At present, species such assoybean and cotton were available as a series of maturity groups thatwere suitable for different latitudes on the basis of their floweringtime (which is governed by day-length). A system in which flowering canbe chemically controlled could allow a single high-yielding northernmaturity group to be grown at any latitude. In southern regions suchplants can be grown for longer, thereby increasing yields, beforeflowering was induced. In more northern areas, the induction can be usedto ensure that the crop flowers prior to the first winter frosts.Currently, the existence of a series of maturity groups for differentlatitudes represents a major barrier to the introduction of new valuabletraits. Any trait (e.g. disease resistance) has to be bred into each ofthe different maturity groups separately; a laborious and costlyexercise. The availability of single strain, which can be grown at anylatitude, could therefore greatly increase the potential for introducingnew traits to crop species such as soybean and cotton.

For many crop species, high yielding winter-varieties can only be grownin temperate regions where the winter season is prolonged and coldenough to elicit a vernalization response. If the vernalizationtreatment can be compensated for by modulating the expression of certaintranscription factors in crop plants, winter varieties of wheat, forinstance, might then be grown in areas like Southern California whichwould otherwise be too warm to allow effective vernalization. Anotherapplication is in cherry (Prunus). Locally grown cherries areunavailable in the early Californian spring since the winters are toowarm for vernalization to occur.

A further application exists in strawberry (Fragaria). Strawberry has awell-defined perennial cycle of flower initiation, dormancy, chilling,crop growth and runner production. In temperate European countries, theplants flower in early spring, and fruit is produced in May or June.Following fruiting, runners are generated that carry plantlets whichtake root. The plants then remain dormant all through the late summerand autumn. Flowering cannot be repeated until the following springafter the plants have received a winter cold treatment. A system thatbypasses this vernalization requirement could permit a second autumncrop of strawberries to be harvested in addition to the spring crop.

G19 (SEQ ID NO: 3)

Published Information

G19 belongs to the EREBP subfamily of transcription factors and containsonly one AP2 domain. G19 corresponds to the previously described geneRAP2.3 (Okamuro et al. (1997) Proc. Natl. Acad. Sci. 94:7076-7081).Close inspection of the Arabidopsis cDNA sequences of RAP2.3 (AF003096;Okamuro et al. (1997) supra), AtEBP (Y09942; Buttner et al. (1997) Proc.Natl. Acad. Sci. 94:5961-5966), and ATCADINP (Z37504) suggests that theymay correspond to the same gene (Riechmann et al. (1998) Biol. Chem.379:633-646). G19/RAP2.3 is ubiquitously expressed (Okamuro et al.(1997) supra). AtEBP was isolated by virtue of the protein-proteininteraction between AtEBP and OBF4, a basic-region leucine zippertranscription factor (Buttner et al. (1997) supra). AtEBP expressionlevels in seedlings were increased after treatment with ethylene(ethephon) (Buttner et al. (1997) supra). AtEBP was found to bind toGCC-box containing sequences, like that of the PRB-1b promoter (Buttneret al. (1997) supra). It has been suggested that the interaction betweenAtEBP and OBF4 reflects cross-coupling between EREBP and bZIPtranscription factors that might be important in regulating geneexpression during the plant defense response (Buttner et al. (1997)supra).

Experimental Observations

Transgenic plants in which G19 is expressed under the control of the 35Spromoter were morphologically similar to control plants. G19 isconstitutively expressed in the different tissues examined; however G19expression was significantly repressed by methyl jasmonate (MeJ) andinduced by ACC (this latter result correlates with the previouslydescribed increase in G19 expression levels in seedlings after treatmentwith ethylene (ethephon); Buttner et al. (1997) supra). G19 wassignificantly induced upon infection by the fungal pathogen Erysipheorontii. In addition, G19 overexpressing plants were more tolerant toinfection with a moderate dose of Erysiphe orontii. G19 overexpressingplants were also tested for their tolerance to two other pathogens, thenecrotrophic fungal pathogen Fusarium oxysporum and the bacterialpathogen Pseudomonas syringae; the transgenic plants were not found tohave altered susceptibility to the pathogens.

Both the jasmonic acid and the ethylene signal transduction pathwayswere involved in the regulation of the defense response and the woundresponse, and the two pathways have been found to interactsynergistically. The regulation of G19 expression by both hormones, itsinduction upon Erysiphe orontii infection, as well as the preliminarydata indicating that increased tolerance to that pathogen was conferredby G19 overexpression, suggest that G19 plays a role in the control ofthe defense and/or wound response. It would be of interest to test G19overexpressing plants in insect-plant interaction experiments. Theincrease in tolerance to Erysiphe orontii that is conferred by G19overexpression can be tested using other races of the pathogen. It wouldalso be of interest to test other pathogens in addition to Erysipheorontii, Fusarium oxysporum, and Pseudomonas syringae.

Since G19 was expressed at significant levels in a constitutive fashion,similar experiments to those described here can be performed with G19knockout mutant plants to further elucidate the function of this gene.

Potential Applications

G19 or its equivalogs can be used to manipulate the plant defense-wound- or insect-response, as well as the jasmonic acid and ethylenesignal transduction pathways themselves.

G22 (SEQ ID NO: 5)

Published Information

G22 was identified in the sequence of BAC T13E15 (gene T13E15.5) by TheInstitute of Genomic Research (TIGR) as a “TINY transcription factorisolog”. G22 belongs to the EREBP subfamily and contains only one AP2domain, and phylogenetic analyses place G22 relatively close to otherEREBP subfamily genes, such as, TINY and ATDL4400C (Riechmann et al.(1998) Biol. Chem. 379:633-646).

Experimental Observations

G22 was constitutively expressed at medium levels. There appeared to beno phenotypic alteration on plant morphology upon G22 overexpression.Plants ectopically overexpressing G22 were more tolerant to high NaClcontaining media in a root growth assay compared with wild-typecontrols.

Potential Applications

G22 or its equivalogs can be used to increase plant tolerance to soilsalinity during germination, at the seedling stage, or throughout theplant life cycle. Salt tolerance is a particularly desirable phenotypeduring the germination stage of a crop plant, which would impactsurvivability and yield.

G24 (SEQ ID NO: 7)

Published Information

G24 corresponds to gene At2g23340 (AAB87098).

Experimental Observations

The function of G24 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G24 caused alterations in plant growth and development. Most notably,35S::G24 seedlings often developed black necrotic tissue patches oncotyledons and leaves, and many died at that stage. Some 35S::G24seedlings exhibited a weaker phenotype, and although necrotic patcheswere visible on the cotyledons, they did not die. These seedlingsdeveloped into plants that were usually small, slow growing, and poorlyfertile in comparison to wild-type controls. The leaves of older35S::G24 plants were also observed to become yellow and senesceprematurely compared with wild type. For those lines that could beassayed in biochemical and physiological assays, no differences wereobserved with respect to wild-type controls.

G24 was ubiquitously expressed, at apparently lower levels ingerminating seedlings.

The AP2 domain of G24 is nearly identical to that of other ArabidopsisEREBP proteins, such as G12, G1379, and G1277.

Potential Applications

G24 or its equivalogs can be used to trigger cell death and influence orcontrol processes in which cell death plays a role. G24 can be used toblock pathogen infection by triggering it in infected cells and blockingspread of the disease.

G28 (SEQ ID NO: 9)

Published Information

G28 corresponds to AtERF1 (GenBank accession number AB008103) (Fujimotoet al. (2000) Plant Cell 12:393-404). G28 appears as gene AT4g17500 inthe annotated sequence of Arabidopsis chromosome 4 (AL161546.2).

AtERF1 has been shown to have GCC-box binding activity [somedefense-related genes that were induced by ethylene were found tocontain a short cis-acting element known as the GCC-box: AGCCGCC (Ohmeet al. (1990) Plant Mol. Biol. 15:941-946)]. Using transient assays inArabidopsis leaves, AtERF1 was found to be able to act as a GCC-boxsequence specific transactivator (Fujimoto et al. (2000) supra).

AtERF1 expression has been described to be induced by ethylene (two- tothree-fold increase in AtERF1 transcript levels 12 h after ethylenetreatment) (Fujimoto et al. (2000) supra). In the ein2 mutant, theexpression of AtERF1 was not induced by ethylene, suggesting that theethylene induction of AtERF1 is regulated under the ethylene signalingpathway (Fujimoto et al. (2000) supra). AtERF1 expression was alsoinduced by wounding, but not by other abiotic stresses (such as cold,salinity, or drought) (Fujimoto et al. (2000) supra).

It has been suggested that AtERFs, in general, may act as transcriptionfactors for stress-responsive genes, and that the GCC-box may act as acis-regulatory element for biotic and abiotic stress signal transductionin addition to its role as an ethylene responsive element (ERE)(Fujimoto et al. (2000) supra), but there is no data available on thephysiological functions of AtERF1.

Experimental Observations

The function of G28 was analyzed using transgenic plants in which thisgene was expressed under the control of the 35S promoter. G28overexpressing lines were more tolerant to infection with a moderatedose of the fungal pathogen Erysiphe orontii. G28 overexpression did notseem to have detrimental effects on plant growth or vigor, since plantsfrom most of the lines were morphologically wild-type. In addition, nodifference was detected between those lines and the correspondingwild-type controls in all the biochemical assays that were performed.

G28 was ubiquitously expressed.

G28 overexpressing lines were also more tolerant to Sclerotiniasclerotiorum and Botrytis cinerea. In a repeat experiment usingindividual lines, all three lines analyzed showed tolerance to S.sclerotiorum, and two of the three lines tested were more tolerant to Bcinerea.

Potential Applications

G28 transgenic plants had an altered response to fungal pathogens, inthat those plants were more tolerant to the pathogens. Therefore, G28 orits equivalogs can be used to manipulate the defense response in orderto generate pathogen-resistant plants.

G47 (SEQ ID NO: 11)

Published Information

G47 corresponds to gene T22118.2 (AAC25505).

Experimental Observations

The function of G47 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G47 resulted in a variety of morphological and physiologicalphenotypic alterations.

35S::G47 plants showed enhanced tolerance to osmotic stress. In a rootgrowth assay on PEG containing media, G47 overexpressing transgenicseedlings were larger and had more root growth compared with thewild-type controls. G47 expression levels can be altered byenvironmental conditions, in particular reduced by salt and osmoticstresses.

Overexpression of G47 also produced a substantial delay in floweringtime and caused a marked change in shoot architecture. 35S::G47transformants were small at early stages and switched to flowering morethan a week later than wild-type controls (continuous light conditions).The inflorescences from these plants appeared thick and fleshy, hadreduced apical dominance, and exhibited reduced internode elongationleading to a short compact stature. The branching pattern of the stemsalso appeared abnormal, with the primary shoot becoming “kinked” at eachcoflorescence node. Additionally, the plants showed reduced fertilityand formed rather small siliques that were borne on short pedicels andheld vertically, close against the stem.

Additional alterations were detected in the inflorescence stems of35S::G47 plants. Stem sections from T2-21 and T2-24 plants were of widerdiameter, and had large irregular vascular bundles containing a muchgreater number of xylem vessels than wild type. Furthermore, some of thexylem vessels within the bundles appeared narrow and were possibly morelignified than were those of controls.

G47 was expressed at higher levels in rosette leaves, and transcriptswere detected in other tissues (flower, embryo, silique, and germinatingseedling), but not in roots.

Potential Applications

G47 or its equivalogs can be used to manipulate flowering time, tomodify plant architecture and stem structure (including development ofvascular tissues and lignin content) and to improve plant performanceunder osmotic stress.

Transcription factor equivalogs that modulate lignin content can bevaluable. This modulation can allow the quality of wood used forfurniture or construction to be improved. Lignin is energy rich;increasing lignin composition is valuable in raising the energy contentof wood used for fuel. Conversely, the pulp and paper industries seekwood with a reduced lignin content. Currently, lignin must be removed ina costly process that involves the use of many polluting chemicals.Consequently, lignin is a serious barrier to efficient pulp and paperproduction. In addition to forest biotechnology applications, changinglignin content can increase the palatability of various fruits andvegetables.

G156 (SEQ ID NO: 13)

Published Information

G156 corresponds to gene MKD15.12 (GenBank accession number BAB11181.1).G156 has also been described as AGL32 (Alvarez-Buylla et al. (2000)Proc. Natl. Acad. Sci. 97:5328-5333). Phylogenetic analyses of theArabidopsis MADS box gene family indicate that G156/AGL32 is a Type IIMADS-box gene, but it does not belong to any of the well-characterizedType II MADS gene clades (Alvarez-Buylla et al. 2000 supra).

Experimental Observations

The complete cDNA sequence of G156 was determined. The function of thisgene was analyzed using both transgenic plants in which G156 wasexpressed under the control of the 35S promoter and a line homozygousfor a T-DNA insertion in the gene. The T-DNA insertion lies in thesecond intron, and was expected to result in a strong loss-of-functionor null mutation.

G156 knockout mutant plants produced yellow seed that showed morevariation in shape than wild type, implying a function (direct orindirect) for G156 in seed development. G156 mutant plants wereotherwise normal at all other developmental stages. Expression of G156was determined to be specific to floral tissues. Although expression wasdetected by RT-PCR in flowers, siliques, and embryos, it could well bethat G156 was specifically expressed in embryo/seed during development,in light of the many MADS box genes that have been shown to be expressedin specific floral organs or cell types, and of the G156 knockout mutantphenotype. In situ RNA hybridization experiments will determine moreprecisely G156 expression pattern.

The coloration phenotype of the G156 knockout mutant seed resembles thatof ttgl and the transparent testa mutants. TTG1, which is localized inChromosome 5, but approximately 0.5 Mb away from the clone that containsG156 (MKD15), codes for a WD40 repeat protein (Walker et al. (1999)Plant Cell 11:1337-1350). The transparent testa (tt) loci wereidentified in screens for mutations that result in yellow or pale brownseeds (Koornneef (1990) Arabidopsis Inf. Ser. 27:1-4). Many of the “TT”genes have been mapped, and several of them have been cloned and shownto be involved in the anthocyanin pathway (Debeaujon et al. (2001) PlantCell 13:853-872)

None of the TT genes corresponds to G156. TT3, TT4, TT5, and TT7 codefor dihydroflavol 4-reductase, chalcone synthase, chalcone flavanoneisomerase, and flavonoid 3′-hydroxylase, respectively (Shirley et al.(1992) Plant Cell 4:333-347; Shirley et al. (1995) Plant J. 8:659-671).TT12 encodes a multidrug secondary transporter-like protein required forflavonoid sequestration in vacuoles of the seed coat endothelium(Debeaujon et al. (2001) supra). TT6 and TT9 map on Chromosome 3, andTT1 maps on Chromosome 1. TT2 and TT10 map on Chromosome 5, but far awayfrom the position of G156 (Shirley et al. (1995) supra). TT8 has alsobeen cloned and shown to encode a transcription factor of the basichelix-loop-helix class (Nesi et al. (2000) Plant Cell 12:1863-1878),providing further evidence for the regulation of the anthocyanin pathwayat the transcriptional level.

The similarity of the G156 knockout and tt seed coloration phenotypes,and the involvement of at least some of the TT genes in the anthocyaninpathway, suggested that G156 is involved in its regulation.

In addition to the seed coloration phenotype, the G156 knockout mutantshowed a significant increase in the percentage of seed 18:1 fattyacids.

G156 overexpressing plants showed a variety of morphologicalalterations, largely uninformative. The most severely affectedtransformants were extremely dwarfed, had aberrant branching, andsometimes possessed terminal flowers. These phenotypic alterations werefrequently observed when MADS box genes that were involved in flowerdevelopment were overexpressed in Arabidopsis (for instance, AG, AP1,and AP3+PI; Mizukami et al. (1992) Cell 71:119-131; Mandel et al. (1995)Nature 377:522-524; Krizek et al. (1996) Development 122:11-22).

Both G156 knockout mutant plants and G156 overexpressing lines behavedlike the wild-type controls in the physiological assays performed.

Potential Applications

G156 or its equivalogs can be used to manipulate the anthocyaninbiosynthetic pathway, such as for altering seed coloration. In addition,the promoter of G156 may be used to confer seed-specific expression togenes of interest.

G157 (SEQ ID NO: 15)

Published Information

G157 was first identified in the sequence of BAC F22K20 (GenBankaccession number AC002291; gene F22K20.15).

Experimental Observations

G157 was recognized as a gene highly related to Arabidopsis FLOWERINGLOCUS C (FLC; Michaels et al. (1999) Plant Cell 11:949-956; Sheldon etal. (1999) Plant Cell 11:445-458). FLC acts as a repressor of flowering.Late flowering vernalization responsive ecotypes and mutants have highsteady state levels of FLC transcript, which decrease during thepromotion of flowering by vernalization. FLC therefore has a centralrole in regulating the response to vernalization (Michaels (1999) supra;Sheldon et al. (1999) supra; Sheldon et al. (2000) Proc. Natl. Acad.Sci. 97:3753-3758).

The function of G157 was studied using transgenic plants in which thisgene was expressed under the control of the 35S promoter.Over-expression of G157 modifies flowering time, and it appears to do soin a quantitative manner: a modest level of over-expression triggersearly flowering, whereas a larger increase delays flowering. G157over-expression promoted flowering in the Arabidopsis late-floweringvernalization-dependent ecotypes Stockholm and Pitztal.

In contrast to FLC, G157 transcript levels showed no correlation withthe vernalization response, and over-expression of G157 did notinfluence FLC transcript levels. Thus, G157 likely acts downstream orindependently of FLC transcription. In addition, a cluster of fouradditional FLC-like and G157-like genes were identified, raising thepossibility that a whole sub-group of proteins within the MADS familyregulates flowering time.

G157 overexpressing plants did not show any other morphological,physiological, or biochemical alteration in the assays that wereperformed. Overexpression of G157 was not observed to have deleteriouseffects: 35S::G157 plants were healthy and attained a wild-type staturewhen mature.

For many crops, high yielding winter strains can only be grown inregions where the growing season is sufficiently cold and prolonged toelicit vernalization. A system that could trigger flowering at highertemperatures would greatly expand the acreage over which wintervarieties can be cultivated. The finding that G157 overexpression causedearly flowering in Arabidopsis Stockholm and Pitztal plants, indicatedthat the gene can overcome the high level of FRIGIDA and FLC activitypresent in those late-ecotypes. That the effects were similar to thosecaused by vernalization implied that G157 might be applicable to winterstrains of crop species. To date, a substantial number of genes havebeen found to promote flowering. Many, however, including those encodingthe transcription factors, APETALA1, LEAFY, and CONSTANS, produceextreme dwarfing and/or shoot termination when over-expressed.Overexpression of G157 was not observed to have deleterious effects.35S::G157 Arabidopsis plants were healthy and attained a wild-typestature when mature. Irrespective of the mode of G157 action, andwhether its true biological role is as an activator or a repressor offlowering, the results suggested that G157 may produce either early orlate flowering, according to the level of over-expression.

G162 (SEQ ID NO: 17)

Published Information

G162 corresponds to gene At2g34440 (AAC26702), and it has also beenreferred to as AGL29.

Experimental Observations

The function of G162 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G162plants were wild-type in morphology and development. Overexpression ofG162 resulted in a significant increase in oil content in seeds, asmeasured by NIR.

Potential Applications

G162 or its equivalogs may be used to increase seed oil content andmanipulate seed protein content in crop plants.

G175 (SEQ ID NO: 19)

Published Information

G175 was identified in the sequence of P1 clone M3E9 (GeneAT4g26440/M3E9.130; GenBank accession number CAB79499).

Experimental Observations

The complete cDNA sequence of G175 was determined. The function of thisgene was studied using transgenic plants in which G175 was expressedunder the control of the 35S promoter. 35S::G175 plants were moretolerant to osmotic stress conditions (better germination in NaCl- andsucrose-containing media). The plants were otherwise wild-type inmorphology and development. Whereas some phenotypic changes weredetected in the biochemical assays that were performed, these were notobserved in more than one line.

G175 appeared to be specifically expressed in floral tissues, and alsoappeared to be induced elsewhere by heat and salt stress.

Potential Applications

G175 or its equivalogs can be used to increase germination under adverseosmotic stress conditions, which could impact survivability and yield.The promoter of G175 may be used to drive flower specific expression.

G180 (SEQ ID NO: 21)

Published Information

G180 was identified in the sequence of BAC F16B22 (GenBank accessionnumber AC003672).

Experimental Observations

The complete sequence of G180 was determined G180 was not annotated inthe sequence of Arabidopsis thaliana chromosome II section 239 of 255 ofthe complete sequence (AC003672.2), where it resides between At2g44740and At2g44750.

The function of G180 was analyzed using transgenic plants in which thisgene was expressed under the control of the 35S promoter.

G180 overexpressing plants were early flowering, but did not exhibitother major developmental alterations. A number of Arabidopsis geneshave already been shown to accelerate flowering when constitutivelyexpressed. These include LEAFY, APETALA1 and CONSTANS. In these cases,however, the early flowering plants showed undesirable side effects suchas extreme dwarfing, infertility, or premature termination of shootmeristem growth (Mandel et al. (1995) Nature 377:522-524; Weigel et al.(1995) 377: 495-500; Simon et al. (1996) Nature 384:59-62). It appearedthat G180 induced flowering without these toxic pleiotropic effects.

G180 overexpressing lines also showed a decrease in seed oil content.That decrease was accompanied increased seed protein content in one ofthe three lines analyzed.

Potential Applications

G180 overexpression appeared to alter flowering time by accelerating thetransition from vegetative to reproductive state. Therefore, G180 or itsequivalogs may be used to manipulate flowering time in plants. Inaddition, G180 or its equivalogs can also have utility in modifying seedtraits, particularly in modifying seed oil and protein levels in cropplants.

G183 (SEQ ID NO: 23)

Published Information

G183 corresponds to gene F20N2.3 (AAF79511).

Experimental Observations

The function of G183 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G183 resulted in an a reduction of the time to flowering.

Under continuous light conditions, 35S::G183 plants formed flower budsapproximately 2-4 days earlier than wild-type control plants. Such aphenotype was seen in two separate plantings and in each of twoindependent T2 lines. Overexpression of G183 also resulted in seedlingswith an altered response to light. In a germination assay conducted indarkness, G183 seedlings failed to show an etiolation response. However,the phenotype was severe in seedlings from one line where overexpressionof the transgene resulted in reduced hypocotyl elongation and open,greenish cotyledons, but this line did not show alterations in floweringtime in the T2 generation.

In addition to the effects on flowering time, 35S::G183 transformantswere generally small, produced rather thin inflorescences, and had a lowseed yield compared with wild type. Such effects were particularlyapparent in some of the T1 plants. It should also be noted that thetransformation rate attained with this transgene was relatively low,suggesting that G183 might have lethal effects at high dosages.Overexpression of G183 did not result in any biochemical phenotypicalteration.

According to the results obtained in the RT-PCR experiments, G183 wasspecifically expressed in flower, embryo, and silique tissues. It shouldbe noted, however, that there have already been cases described ofArabidopsis transcription factor genes that were specifically expressedin flower-derived tissues but that can affect flowering time when theirexpression pattern is modified, including a homeobox gene longconsidered representing a true flowering time locus, FWA.

Potential Applications

G183 or its equivalogs may be used to modify flowering time and lightresponse.

G183 or its equivalogs may alter a plant's light response and thusmodify growth or development, for example, photomorphogenesis in poorlight, or accelerating flowering time in response to various lightintensities, quality or duration to which a non-transformed plant wouldnot similarly respond, and increased planting densities with subsequentyield enhancement.

G188 (SEQ ID NO: 25)

Published Information

G188 corresponds to gene MXC20.3, first identified in the sequence ofclone MXC20 (released by the Arabidopsis Genome Initiative; GenBankaccession number AB009055).

Experimental Observations

The annotation of G188 in BAC AB009055 was experimentally confirmed.G188 was expressed in all tissues and under all conditions examined.

A line homozygous for a T-DNA insertion in G188 was initially used tocharacterize the function of this gene. In such line, the T-DNAinsertion in G188 was localized in the second intron of the gene,located in the middle of the conserved WRKY box. Such insertion wouldresult in a null mutation (unless the large fragment of exogenoussequence is perfectly spliced out from the transcribed G188 pre-mRNA).G188 mutant plants displayed several phenotypic alterations inphysiological assays. G188 knockout mutant seed germinated better thanwild-type controls under several kinds of osmotic stress. G188 knockoutplants also showed higher susceptibility to the necrotroph fungalpathogen Fusarium oxysporum compared with control plants (more diseasespread after infection). No significant morphological changes wereobserved in G188 knockout plants.

The function of G188 was subsequently analyzed using transgenic plantsin which the gene was expressed under the control of the 35S promoter.G188 overexpressing plants were morphologically wild-type, andindistinguishable from the corresponding controls in all physiologicaland biochemical assays that were performed. Overexpression of G188 didnot increase resistance to Fusarium oxysporum.

Further experiments to characterize G188 function can include testingthe plant (knockout or overexpressor) to different doses of the pathogenFusarium oxysporum, as well as more sophisticated gene expressionprofiling experiments.

Potential Applications

G188 or its equivalogs can be used to enhance seed germination underadverse osmotic conditions. G188 appears to be involved in the plant'sresponse to Fusarium oxysporum and thus it may be used to manipulatesuch responses.

G189 (SEQ ID NO: 27)

Published Information

G189 was identified in the sequence of BAC clone T20D16 (geneAt2g23320/T20D16.5, GenBank accession number AAB87100).

Experimental Observations

The function of G189 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. T1 G189overexpressing plants showed leaves of larger area than wild type. Thisphenotype, which was observed in two different T1 plantings, became moreapparent at late vegetative development. T2 plants were morphologicallywild-type. In wild-type plants, G189 was constitutively expressed.

G189 overexpressing plants were wild-type in all the physiologicalanalyses performed.

Potential Applications

G189 or its equivalogs can be used to increase plant biomass. Large sizeis useful in crops where the vegetative portion of the plant is themarketable portion since vegetative growth often stops when plants makethe transition to flowering.

G192 (SEQ ID NO: 29)

Published Information

G192 corresponds to gene A_IG002N01.6, first identified in the sequenceof BAC clone A_IG002N01 (released by the Arabidopsis Genome Initiative;GenBank accession number AF007269).

Experimental Observations

The annotation of G192 in BAC AF007269 was experimentally confirmed.G192 was expressed in all plant tissues and under all conditionsexamined. Its expression was induced upon infection by Fusarium.

The function of G192 was analyzed using transgenic plants in which thisgene was expressed under the control of the 35S promoter. G192overexpressors were late flowering under 12 hour light and had moreleaves than control plants. This phenotype was manifested in the threeT2 lines analyzed. In addition, one line showed a decrease in seed oilcontent. No other differences between G192 overexpressing lines andcontrol plants were noted in the assays performed.

A decrease in seed oil observed previously in one transgenic line wasreplicated in an independent experiment.

Potential Applications

G192 overexpression delayed flowering. A wide variety of applicationsexist for genes or their equivalogs that either lengthen or shorten thetime to flowering, or for systems of inducible flowering time control.In particular, in species where the vegetative parts of the plantsconstitute the crop and the reproductive tissues were discarded, itwould be advantageous to delay or prevent flowering. Extendingvegetative development may bring about large increases in yields.

G192 or its equivalogs can be used to manipulate seed oil content, whichmight be of nutritional value.

G196 (SEQ ID NO: 31)

Published Information

G196 corresponds to gene At2g34830 (AAC12823).

Experimental Observations

The function of G196 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G196plants show more tolerance to salt stress in a germination assay.Overexpression of G196 also produced a range of effects on plantmorphology including a reduction in overall size, lowered fertility andchanges in leaf shape. T1 seedlings were typically small, often hadabnormal shaped cotyledons, and the rosette leaves produced by theseplants were often undersized, contorted and darker green compared withwild type. Later in development, during the reproductive stage, theplants formed thin inflorescences bearing poorly fertile flowers withunderdeveloped organs. 35S::G196 primary transformants were obtained ata relatively low frequency, suggesting that the gene might have lethaleffects if overexpressed at very high levels.

35S::G196 plants were wild-type in the biochemical analyses that wereperformed. G196 was ubiquitously expressed (and different levels amongthe various tissues).

Potential Applications

G196 or its equivalogs may be used to improve plant performance underconditions of salt stress. Evaporation from the soil surface causesupward water movement and salt accumulation in the upper soil layerwhere the seeds were placed. Thus, germination normally takes place at asalt concentration that is higher than the mean salt concentration inthe whole soil profile. Increased salt tolerance during the germinationstage of a crop plant may impact survivability and yield.

G211 (SEQ ID NO: 33)

Published Information

G211 corresponds to Atmyb5 (U26935; Li et al. (1996) FEBS Lett379:117-121). Arabidopsis plants transgenic for a chimeric Atmyb5promoter/GUS gene expressed the enzyme in developing leaf trichomes,stipules, epidermal cells on the margins of young rosette and caulineleaves, and in immature seeds. In immature seeds, Atmyb5 expressionoccurs between fertilization and the 16 cell stage of embryo developmentand persists beyond the heart stage.

Experimental Observations

The function of G211 was investigated using a homozygous mutant line inwhich a T-DNA was inserted into the coding region of the gene as well asusing transgenic lines in which G211 is expressed under the control ofthe 35S promoter. The phenotype of the G211 knockout mutant plants waswild-type in all respects. Overexpression of G211, however, had markedeffects on leaf and inflorescence development. 35S::G211 plants weregenerally small, slow developing, and produced rounded, slightlyserrated leaves, with very short petioles. Additionally these plantswere dark green in coloration, and in some cases, appeared to havereduced trichome density. Following the switch to reproductive growth,35S::G211 inflorescences had short internodes and showed a generalreduction in apical dominance, leading to a bushy appearance. In manycases, due to the small size, seed yield was reduced compared withwild-type controls. These effects were highly penetrant and wereapparent in the majority of T1 lines and, to some extent, in each of thethree T2 populations. An increase in leaf xylose in two lines was alsoobserved in the T2 35S::G211 transgenics.

As determined by RT-PCR, expression of G211 was found primarily inembryos and siliques. G211 expression in leaf tissue was unaffected byany environmental stress-related condition tested.

Potential Applications

G211 overexpression resulted in plants with altered leaf insoluble sugarcontent. Transcription factors such as G211 or their equivalogs thatalter plant cell wall composition have several potential applicationsincluding altering food digestibility, plant tensile strength, woodquality, pathogen resistance and in pulp production.

In particular, hemicellulose is not desirable in paper pulps because ofits lack of strength compared with cellulose. Thus, modulating theamounts of cellulose vs. hemicellulose in the plant cell wall isdesirable for the paper/lumber industry. Increasing the insolublecarbohydrate content in various fruits, vegetables, and other edibleconsumer products will result in enhanced fiber content. Increased fibercontent would not only provide health benefits in food products, butmight also increase digestibility of forage crops. In addition, thehemicellulose and pectin content of fruits and berries affects thequality of jam and catsup made from them. Changes in hemicellulose andpectin content could result in a superior consumer product.

G214 (SEQ ID NO: 35)

Published Information

G214 (CCA1) was published by Wang et al. (1997) Plant Cell 9: 491-507.CCA1 is involved in phytochrome induction of CAB genes. The transcriptis transiently induced by phytochrome and oscillates with a circadianrhythm. It feedback-regulates its own expression at the transcriptionallevel. Overexpressing CCA1 abolished circadian rhythm of several genesand results in plants that were late flowering, and have elongatedhypocotyls.

Experimental Observations

G214 overexpressing lines were late bolting, show larger biomass(increased leaf number and size), and were darker green in vegetativeand reproductive tissues due to a higher chlorophyll content in thelater stages of development. In these later stages, the overexpressorsalso have higher insoluble sugar, leaf fatty acid, and carotenoidcontent per unit area. Line #11 also showed a significant, repeatableincrease in lutein levels in seeds. Microarray data was consistent withthe morphological and biochemical data in that the genes that werehighly induced included chloroplast localized enzymes, and lightregulated genes such as Rubisco, carbonic anhydrase, and the photosystem1 reaction center subunit precursor. A chlorophyll biosynthetic enzymewas also highly induced, consistent with the dark green color of theadult leaves and perhaps a higher photosynthetic rate. A measurement ofleaf fatty acid in the older overexpressors suggested that the overalllevels were higher than wild-type levels (except for the percentcomposition of 16:3 in line #11). Percent composition of 16:1 and 16:3fatty acids (found primarily in plastids) is similar to wild typearguing against an increase in chloroplast number as an explanation forincrease chlorophyll content in the leaves. Three G214-overexpressinglines were sensitive to germination on high glucose showing lesscotyledon expansion and hypocotyl elongation suggesting the late boltingand dark green phenotype could be tied into carbon sensing which hasbeen shown to regulate phytochrome A signaling (Dijkwel et al. (1997)Plant Cell 9:583-595; Van Oosten et al. (1997) Plant J. 12:1011-1020).Sugars are key regulatory molecules that affect diverse processes inhigher plants including germination, growth, flowering, senescence,sugar metabolism and photosynthesis. Glucose-specific hexose-sensing hasalso been described in plants and implicated in cell division and therepression of famine genes (photosynthetic or glyoxylate cycles).

Potential Applications

Potential utilities of this gene or its equivalogs include increasingchlorophyll content allowing more growth and productivity in conditionsof low light. With a potentially higher photosynthetic rate, fruits canhave higher sugar content. Increased carotenoid content may be used as anutraceutical to produce foods with greater antioxidant capability. G214or its equivalogs can also be used to manipulate seed composition, whichis very important for the nutritional value and production of variousfood products.

G214 overexpression delayed flowering time in transgenic plants, andthus this gene or its equivalogs would be useful in modifying floweringtime. In a sizeable number of species, for example, root crops, wherethe vegetative parts of the plants constitute the crop and thereproductive tissues were discarded, it is advantageous to identify andincorporate transcription factor genes that delay or prevent floweringin order to prevent resources being diverted into reproductivedevelopment. Extending vegetative development can thus bring about largeincreases in yields.

G226 (SEQ ID NO: 37)

Published Information

G226 was identified from the Arabidopsis BAC sequence, AC002338, basedon its sequence similarity within the conserved domain to other Mybfamily members in Arabidopsis. To date, there is no publishedinformation regarding the function of this gene.

Experimental Observations

The function of G226 was analyzed through its ectopic overexpression inplants. G226 overexpressors were more tolerant to low nitrogen and highsalt stress. They showed more root growth and possibly more root hairsunder conditions of nitrogen limitation compared with wild-typecontrols. Many plants were glabrous and lacked anthocyanin productionwhen under stress such as growth conditions of low nitrogen and highsalt. Several G226 overexpressors were glabrous and produce lessanthocyanin under stress; these effects might be due to binding sitecompetition with other Myb family transcription factors involved inthese functions and not directly related to the primary function of thisgene.

Results from the biochemical analysis of G226 overexpressors suggestedthat one line had higher amounts of seed protein, which could have beena result of increased nitrogen uptake by these plants.

A microarray experiment was done on a separate G226 overexpressing line.The G226 sequence itself was overexpressed 16-fold above wild type,however, very few changes in other gene expression were observed in thisline. On the array, a chlorate/nitrate transporter DNA sequence wasinduced 2.7-fold over wild type, which could explain the low nitrogentolerant phenotype of the plants and the increased amounts of seedprotein in one of the lines. The same DNA sequence was present severaltimes on the array and in all cases the DNA sequence showed induction,adding more validity to the data. Five other genes/DNA sequences inducedbut had unknown function. A methyltransferase, a pollen-specificprotein, and a zinc binding peroxisomal membrane protein encodingsequences were also induced, however their role in regard to thephenotype of the plants is not known.

Potential Applications

The utilities of a gene or its equivalogs conferring tolerance toconditions of low nitrogen include: (1) Cost savings to the farmer byreducing the amounts of fertilizer needed; (2) Environmental benefits ofreduced fertilizer runoff; (3) Improved yield and stress tolerance. Inaddition, G226 can be used to increase seed protein amounts and/orcomposition, which may impact yield as well as the nutritional value andproduction of various food products.

G226 or its equivalogs can be used to alter trichome number anddistribution in plants. Trichome glands on the surface of many higherplants produce and secrete exudates, which give protection from theelements and pests such as insects, microbes and herbivores. Theseexudates may physically immobilize insects and spores, may beinsecticidal or antimicrobial or they may allergens or irritants toprotect against herbivores. It has also been suggested that trichomesmay decrease transpiration by decreasing leaf surface airflow, and byexuding chemicals that protect the leaf from the sun.

G241 (SEQ ID NO: 39)

Published Information

G241 is equivalent to Y19 (X90384), a putative light regulated Myb thatwas identified by Quaedvlieg et al. (1996) Plant Mol. Biol. 32:987-993.The Myb Consortium renamed this gene MYB15 and found that it wasconstitutively expressed at a low level with expression higher inetiolated seedlings (Kranz et al. (1998) Plant J. 16:263-276).

Experimental Observations

The function of G241 was analyzed through its ectopic overexpression inplants as well as through the analysis of a line homozygous for aknockout mutation in G241. The knockout mutant plants were wild-type inall assays performed. G241 overexpressors had a glucose germinationphenotype suggesting these plants could be involved in glucose-specificsugar sensing.

Results from the biochemical analysis of G241 knockouts showed that alower amount of seed oil and an increase in seed protein.

RT-PCR analysis of the endogenous levels of G241 showed the gene isexpressed in all tissue types tested.

Results from an array experiment using a G241 overexpressor line wereconsistent with expression in seeds. Several gene sequences were inducedthat could be involved in osmotic stress tolerance or desiccationtolerance, which are important for germinating seeds. In thisexperiment, the G241 DNA sequence itself was induced 38-fold. Many ofthe induced genes were transcription factors with unknown function. BothCBF1 and CBF2 (involved in freezing tolerance) were up-regulated. Asmentioned above, several genes indicative of osmotic stress tolerancewere also up-regulated. These same gene sequences were up-regulated onarrays of plants treated with mannitol as an osmotic stress, in a CBF2overexpressor, and in cold-acclimated plants. A glucose transportersequence was also up-regulated, however, this gene sequence is notup-regulated in any of the other arrays mentioned above. The phenotypeof the overexpressor was reduced seedling growth on high glucose. It ispossible that the plants were taking up more glucose. In such ascenario, the gene is not likely to be involved in sugar sensing butrather the high glucose condition is inhibiting their growth. The G241overexpressors were tested for osmotic stress tolerance using mannitol.It is possible the glucose transporter is increasing mannitol uptake andincreasing its toxicity to the plant as well. Polyethylene glycol (PEG)is an alternative osmoticum that can be tested at variousconcentrations.

Potential Applications

One potential utility of this gene or its equivalogs can be to engineerplants that are tolerant to stress. This can greatly impact yield.Alternatively, if this gene is involved in sugar sensing, the potentialutility of a gene involved in glucose-specific sugar sensing is to alterenergy balance, photosynthetic rate, biomass production, and senescence.Sugars are key regulatory molecules that affect diverse processes inhigher plants including germination, growth, stress responses,flowering, senescence, sugar metabolism and photosynthesis.Glucose-specific hexose-sensing has been described in plants andimplicated in cell division, and repression of famine genes(photosynthetic or glyoxylate cycles). This gene may also be used toalter oil and protein production in seeds, which may be very importantfor the nutritional quality and caloric content of foods.

G248 (SEQ ID NO: 41)

Published Information

G248 was identified at Mendel Biotechnology. Kranz et al. ((1998) PlantJ. 16:263-276) published a cDNA sequence corresponding to G248, namingit MYB22.

Experimental Observations

The function of G248 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. The phenotypeof these transgenic plants was wild-type with respect to theirmorphology. However, overexpression of G248 in Arabidopsis was found toconfer greater sensitivity to disease, particularly following infectionby Botrytis cinerea. All three lines show the susceptible phenotype.

As determined by RT-PCR, G248 appears to be expressed at low levels inembryo and silique tissue. No expression was detected in other tissues.G248 appears to be induced in response to salicylic acid (SA) treatment.It is well know that both synergistic and antagonistic crosstalk betweengrowth regulator controlled defense pathways occurs in response todisease.

Potential Applications

Since G248 transgenic plants had an altered response to the fungalpathogen Botrytis cinerea, G248 or its equivalogs can be used tomanipulate the defense response in order to generate pathogen-resistantplants.

G254 (SEQ ID NO: 43)

Published Information

G254 was identified from the Arabidopsis BAC sequence, AF007269, basedon its sequence similarity within the conserved Myb domain to other Mybfamily members in Arabidopsis.

Experimental Observations

The function of G254 was analyzed through the ectopic overexpression ofthe gene in plants. Overexpression of G254 resulted in a reduction ofgermination and reduced seedling growth on glucose containing media.G254 may be involved in sugar sensing.

RT-PCR analysis of the endogenous levels of G254 indicated that thisgene was expressed in all tissues tested. A cDNA microarray experimentsupported the tissue distribution data by RT-PCR. There was no inductionof G254 above its basal level in response to environmental stresstreatments. G254 was constitutively expressed.

Potential Applications

The potential utility of G254 or its equivalogs is to alter source-sinkrelationships in the plant. Sugars are key regulatory molecules thataffect diverse processes in higher plants including germination, growth,flowering, senescence, sugar metabolism, and photosynthesis. Sucrose isthe major transport form of photosynthate and its flux through cells hasbeen shown to affect gene expression and alter storage compoundaccumulation in seeds (source-sink relationships). The potentialutilities of a gene involved in glucose-specific sugar sensing are toalter energy balance, photosynthetic rate, carbohydrate accumulation,biomass production, source-sink relationships, and senescence.Glucose-specific hexose-sensing has been described in plants andimplicated in cell division and the repression of ‘famine’ genes(photosynthetic or glyoxylate cycles).

G256 (SEQ ID NO: 45)

Published Information

G256 is equivalent to Y13, a gene that was identified by Quaedvlieg etal. ((1996) Plant Mol. Biol. 32:987-093) as being induced in etiolatedseedlings one hour after being exposed to light. The Myb consortium hasrenamed this gene MYB31. Quaedvlieg et al. (1996, supra) found a lowlevel of expression in stem and silique tissue with no induction inetiolated seedlings after being exposed to light. However, there wasalso a slight induction of G256 following cold treatment.

Experimental Observations

The function of G256 was analyzed through its ectopic overexpression inplants. G256 overexpressors had enhanced seedling vigor during coldgermination. These overexpressing lines were more tolerant to chillingconditions compared to wild-type controls, as seen in 12-day-oldseedlings that were transferred to cold temperatures (8° C.).

There was no difference in germination rate under normal growthconditions. The chilling tolerant phenotype is most noticeable withrespect to enhanced root growth although the cotyledons show lessanthocyanin production than wild-type controls.

Plants overexpressing G256 were also small and early bolting. In the T2,one line lacked the waxy surface on the bolts. Three lines were tolerantto cold germination and therefore co-suppression was not a likely causeof the morphological change observed in one line. An array experimentwas performed on this G256 overexpressing line. The gene itself wasinduced 3.5-fold over wild-type levels. Very few additional genesequences were significantly induced in response to G256 overexpression.Induced genes included four gene sequences of unknown function, a sugarcarrier sequence, a cell wall degrading enzyme (BGL2) sequence,pectinesterase sequence, and a proteasome subunit protein sequence.Expression of gene sequences such as allene oxidase sequence (whichcould mean down-regulation of the associated jasmonate synthesispathway), and endochitinase were repressed. RT-PCR analysis of theendogenous levels of G256 indicated that this gene sequence wasexpressed primarily in shoots, flowers, and siliques. A cDNA microarrayexperiment confirmed this tissue distribution data by RT-PCR. There wasno induction of G256 in leaves or in seedlings in response toenvironmental stress treatments.

Potential Applications

The potential utility of this gene or its equivalogs is to confer bettergermination and growth in the cold. The germination of many crops isvery sensitive to cold temperatures. A gene that would allow germinationand seedling vigor in the cold would have tremendous utility in allowingseeds to be planted earlier in the season with a high rate ofsurvivability.

G278 (SEQ ID NO: 47)

Published Information

G278 was identified by amino acid sequence similarity to plant andmammalian ankyrin-repeat proteins. G278 is on chromosome 1 BAC F15H21(GenBank accession number AC066689.5; nid=12323462), released by theArabidopsis Genome Initiative. The transcription start/stop codon wascorrectly predicted. G278 is referred to in the public literature asNPR1, a gene that controls the onset of systemic acquired resistance inplant (Cao et al. (1997) Cell 88:57-63; Cao et al. (1998) Proc. Natl.Acad. Sci. 95:6531-6536).

It was shown that a 2-3-fold overexpression of 35S::NPR1 over basalwild-type expression level results in transgenic plants resistant to thebacterial pathogen Psm ES4326 and the oomycete Peronospora parasiticaNoco. An inducing signal (SA, INA, or a pathogen infection) is necessaryfor the expression of the phenotype and downstream induction ofpathogen-related proteins.

Experimental Observations

RT-PCR analysis of the endogenous level of G278 transcripts revealedthat G278 was present at moderate, constitutive level in all tissuesexamined G278 expression levels were similar to the wild-type control inall the biotic/abiotic treatments examined. The function of G278 wasanalyzed in transgenic plants overexpressing G278 under the control ofthe 35S promoter. Transformants were morphologically indistinguishablefrom wild-type plants. Plants overexpressing G278 were more susceptibleto infection with the necrotrophic fungal pathogen Sclerotiniasclerotiorum when compared with control plants. The experiment wasconfirmed on individual lines. Transgenic G278 overexpressing lines weresimilar to control wild-type plant when challenged with Botrytis cinereaand Fusarium oxysporum.

Data in the public literature indicated that G278/NPR1 plays animportant role in mediating the onset of systemic acquired resistance inplant. Dual resistance of a 35S::NPR1 transgenic plants to bacterial andfungal pathogen suggested that G278/NPR1 may be key to the generation ofbroad-spectrum resistance in plant. The 35S::G278 overexpressorexperimental data indicated that overexpressing G278 had little effectin improving resistance following infection with necrotrophic pathogens(Fusarium oxysporum, Botrytis cinerea, and Sclerotinia sclerotiorum). Infact, reduced tolerance of the transgenic 35S::NPR1 plant to infectionwith Sclerotinia sclerotiorum was observed. Although we cannot rule outthe possibility of co-suppression in transgenic T2 lines tested, it islikely that that resistance to necrotrophic pathogens is mediated bydifferent pathway than the SA/SAR pathway. Overexpression of G278 maydirectly or indirectly introduces competition for co-factor(s) orresults in biochemical interference, which may be detrimental for properdevelopment of resistance to Sclerotinia sclerotiorum.

Potential Applications

35S::G278 overexpression in Arabidopsis was shown to affect the onset ofdisease following inoculation with Sclerotinia sclerotiorum. Therefore,G278 or its equivalogs can be used to manipulate the defense response inplants.

G291 (SEQ ID NO: 49)

Published Information

G291 is referred to in the public literature as the Arabidopsis AJH1, aplant homolog of the c-Jun coactivator. AJH1 was isolated by peptidesequencing of a subunit of the COP9 complex, an important component inlight-mediated signal transduction in Arabidopsis. It is postulated thatthe COP9 complex may modulate the activities of transcription factors inresponse to environmental stimuli. Localization experiment reveals thatAJH1 was present in monomeric form, which suggested a possibleinvolvement in other developmentally regulated processes (Kwok et al.(1998) Plant Cell 10:1779-1790). G291 is found in the sequence of thechromosome 1 BAC F19G10 (GenBank accession AF000657.1 GI:2098816),released by the Arabidopsis Genome Initiative. The start and stop codonswere correctly predicted.

Experimental Observations

The expression profile of G291 revealed a low, but constitutive,expression of G291 transcripts in all tissues examined G291 transcriptlevels were similar to the wild-type controls in all the physiologicaltreatments examined as determined by RT-PCR analysis.

G291 overexpressors produced significantly more seed oil than wild-typeplants.

Potential Applications

G291 or its equivalogs can be used to increase seed oil content, whichmay be of nutritional value for food for human consumption as well asanimal feeds.

G303 (SEQ ID NO: 51)

Published Information

G303 corresponds to gene MNA5.5 (BAB11554.1).

Experimental Observations

The complete sequence of G303 was determined G303 was detected at verylow levels in roots and rosette leaves.

The function of this gene was analyzed using transgenic plants in whichG303 was expressed under the control of the 35S promoter. G303overexpressing plants had more tolerance to osmotic stress in agermination assay in three separate experiments. They had more seedlingvigor than wild-type control when germinated on plates containing highsalt and high sucrose. No altered morphological or biochemicalphenotypes were detected in G303 overexpressing plants.

Potential Applications

G303 or its equivalogs may be useful for enhancing seed germinationunder high salt conditions or other conditions of osmotic stress.Evaporation from the soil surface causes upward water movement and saltaccumulation in the upper soil layer where the seeds are placed. Thus,germination normally takes place at a salt concentration much higherthan the mean salt concentration in the whole soil profile. Increasedsalt tolerance during the germination stage of a crop plant would impactsurvivability and yield. G303 can also be used to engineer plants withenhanced tolerance to drought, salt stress, and freezing.

G312 (SEQ ID NO: 53)

Published Information

G312 corresponds to a predicted SCWERECROW gene regulator in annotatedP1 clone MUD21 (AB010700), from chromosome 5 of Arabidopsis (Kaneko etal. (1998) DNA Res. 5: 131-145).

Experimental Observations

The function of this gene was analyzed using transgenic plants in whichG312 was expressed under the control of the 35S promoter. Transgenicplants overexpressing G312 were more salt tolerant than wild-typeplants, as determined by a germination assay on MS media supplementedwith 150 mM NaCl. G312 was constitutively expressed at very low levelsin all tissues tested. Expression of G312 did not appear to be inducedby any of the environmental or stress conditions tested.

Potential Applications

Transgenic plants overexpressing G312 germinated better in a high saltenvironment than control plants. These data suggested that G312 or itsequivalogs can be used to create crop plants that are more tolerant ofhigh salt conditions. Better germination in high salt conditions isdesirable because, in the field, germination normally takes place at asalt concentration much higher than the mean salt concentration in thewhole soil profile. This is because evaporation from the soil surfacecauses upward water movement and salt accumulation in the upper soillayer where the seeds are placed. Increased salt tolerance during thegermination stage of a crop plant would impact survivability and yield.

G325 (SEQ ID NO: 55)

Published Information

G325 was identified as a gene in the sequence of chromosome 4, ESSA IFCA contig fragment No. 3 (GenBank Accession number Z97338), released bythe European Union Arabidopsis Sequencing Project.

Experimental Observations

The function of G325 was analyzed using transgenic plants in which G325was expressed under the control of the 35S promoter. G325 overexpressingplants had more tolerance to osmotic stress in a germination assay inthree separate experiments. They had more seedling vigor than wild-typecontrol when germinated on plates containing high salt and high sucrose.No altered morphological phenotypes or altered phenotypes in thebiochemical assays were observed.

G325 was expressed at high levels in flowers and cauline leaves, and atlower levels in shoots, rosette leaves, and seedlings. G325 was inducedby auxin, cold- and heat-stress. The expression of G325 also was reducedin response to Fusarium infection or salicylic acid treatment.

Potential Applications

G325 or its equivalogs may be useful for enhancing seed germinationunder high salt conditions or other conditions of osmotic stress.Evaporation from the soil surface causes upward water movement and saltaccumulation in the upper soil layer where the seeds are placed. Thus,germination normally takes place at a salt concentration much higherthan the mean salt concentration in the whole soil profile. Increasedsalt tolerance during the germination stage of a crop plant would impactsurvivability and yield.

G325 or its equivalogs can also be used to engineer plants with enhancedtolerance to drought, salt stress, and freezing, at later stages.

G343 (SEQ ID NO: 59)

Published Information

G343 was identified as GATA-2 (accession number Y13649) by homology toother GATA transcription factors.

Experimental Observations

RT-PCR and microarray data analysis of the endogenous levels of G343indicated that this gene was ubiquitously expressed in all tissuesalbeit predominantly in seedling. In addition, G343 was repressed inresponse to treatment with Erysiphe and Fusarium.

The function of G343 was analyzed through its ectopic overexpression inplants. G343 overexpressors grew very poorly on soil, and T2 plants werenot propagated for biochemical analysis. On the other hand, G343overexpressors grew as well as wild-type controls on MS media, wherethey exhibited an increase in tolerance to glyphosate and oxidativestress. G343 T2 plants were rescued from the control plates, propagatedto the next generation, and tested again on glyphosate plates where theyexhibited the same resistance phenotype. Additional T2 lines wereobtained and tested. A fourth line also showed a striking tolerance toglyphosate, though two other lines exhibited a slight increase insusceptibility to glyphosate. These opposite effects in the T2 linesmight be caused by silencing of the gene. It would, therefore, be veryinteresting to determine the phenotype of G343 knockouts inglyphosate-resistance assays.

Potential Applications

G343 or its equivalogs can be used for the generation of glyphosateresistant plants, and to increase plant resistance to oxidative stress.

G353 (SEQ ID NO: 59)

Published Information

G353 was identified in the sequence of P1 clone MMN10, GenBank accessionnumber AB0154751, released by the Arabidopsis Genome Initiative. G353corresponds to RHL41 (Kazuoka et al. (2000) Plant J. 24:191-203) andZat12 (Meissner et al. (1997) Plant Mol. Biol. 33:615-624). TransgenicArabidopsis plants over-expressing the RHL41 gene showed an increasedtolerance to high-intensity light, and also morphological changes ofthicker and dark green leaves. The palisade parenchyma was highlydeveloped in the leaves of the transgenic plants. Anthocyanin content,as well as the chlorophyll content, also increased. Antisense transgenicplants exhibited decreased tolerance to high irradiation. RHL41 proteinmay play a key role in the acclimatization response to changes in lightintensity.

Experimental Observations

G353 was uniformly expressed in all tissues and under all conditionstested in RT-PCR experiments. The highest level of expression wasobserved in rosette leaves, embryos, and siliques. The function of thisgene was analyzed using transgenic plants in which G353 was expressedunder the control of the 35S promoter. Overexpression of G353 inresulted in enhanced tolerance to osmotic stress in one transgenic line.The most dramatic effect of overexpression of G353 was observed inflower morphology. 35S::G353 plants had a reduction in flower pedicellength, and downward pointing siliques. This phenotype was very similarto that described for the brevipedicellus (bp) mutant (Koornneef et al.(1983) J. Hered. 74:265-272) and in overexpression of a related gene,G354. Other morphological changes in shoots were also observed in35S::G353 plants. Leaves had short petioles, were rather flat, rounded,and sometimes showed changes in coloration. These effects were observedin varying degrees in the majority of transformants. Severely affectedplants were tiny, had contorted leaves, poor fertility, and produced fewseeds. Overexpression of G353 in Arabidopsis resulted in an increase inseed glucosinolate M39494 in two T2 lines.

Potential Applications

G353 or its equivalogs can be used to alter inflorescence structure,which may have value in production of novel ornamental plants.

G353 or its equivalogs can be used to alter a plant's response to waterdeficit conditions and, therefore, be used to engineer plants withenhanced tolerance to drought, salt stress, and freezing.

Increases or decreases in specific glucosinolates or total glucosinolatecontent may be desirable depending upon the particular application. Forexample: (1) Glucosinolates are undesirable components of the oilseedsused in animal feed, since they produce toxic effects. Low-glucosinolatevarieties of canola have been developed to combat this problem; (2) Someglucosinolates have anti-cancer activity; thus, increasing the levels orcomposition of these compounds might be of interest from a nutraceuticalstandpoint; (3) Glucosinolates form part of a plants natural defenseagainst insects; modification of glucosinolate composition or quantitycould therefore afford increased protection from predators; furthermore,in edible crops, tissue specific promoters might be used to ensure thatthese compounds accumulate specifically in tissues, such as theepidermis, which are not taken for consumption.

G354 (SEQ ID NO: 61)

Published Information

G354 was identified in the sequence of BAC clone F12M12, GenBankaccession number AL355775, released by the Arabidopsis GenomeInitiative. G354 corresponds to ZAT7 (Meissner et al. Plant Mol. Biol.33:615-624).

Experimental Observations

Greatest levels of expression of G354 were observed in rosette leaves,embryos, and siliques. Some expression of G354 was also observed inflowers.

The function of this gene was analyzed using transgenic plants in whichG353 was overexpressed under the control of the 35S promoter. 35S::G354plants had a reduction in flower pedicel length, and downward pointingsiliques. This phenotype was very similar to that described for thebrevipedicellus (bp) mutant (Koornneef et al. (1983) J. Hered.74:265-272) and in overexpression of a related gene, G353. Othermorphological changes in shoots were also observed in 35S::G354 plants.Many 35S::G354 seedlings had abnormal cotyledons, elongated, thickenedhypocotyls, and short roots. The majority of T1 plants had a veryextreme phenotype, were tiny, and arrested development without forminginflorescences. T1 plants showing more moderate effects had poor seedyield.

Overexpression of G354 in Arabidopsis resulted in seedlings with analtered response to light. In darkness, G354 seedlings failed toetiolate. The phenotype was most severe in seedlings from one line whereoverexpression of the transgene resulted in reduced open and greenishcotyledons.

Potential Applications

G354 or its equivalogs can be used to alter inflorescence structure,which may have value in production of novel ornamental plants.

G354 modifies the light response and thus G354 or its equivalogs may beuseful for modifying plant growth or development, for example,photomorphogenesis in poor light, or accelerating flowering time inresponse to various light intensities, quality or duration to which anon-transformed plant would not similarly respond. Elimination ofshading responses may lead to increased planting densities withsubsequent yield enhancement.

G361 (SEQ ID NO: 63)

Published Information

G361 was first isolated by Tague et al. ((1995) Plant Mol. Biol.28:267-279) in an effort to study the sequence and the expressionpattern of C2H2 zinc finger protein encoding genes in Arabidopsis(Takatsuji (1998) Cell. Mol. Life. Sci. 54:582-596). The latter studyshowed that G361 (ZFP6) was mostly expressed in roots and shoots basedon Northern analysis.

Experimental Observations

A full-length cDNA was isolated and used to transform plants. G361overexpressors were small and very late bolting. The plants did not showany physiological phenotype. G361 overexpressing plants had increasedlevels of polyunsaturated fatty acids. The phenotype could be related tothe darker green color of the plants and their possible higherchlorophyll content (repeat of analysis also in progress). Higher 16:3fatty acid content, in particular, could be a reflection of a higherchloroplast number or more chloroplast membranes. RT-PCR data showedthat the gene was expressed mostly in shoots and in roots at low levels.

Potential Applications

The late-flowering phenotype of G361 or its equivalogs is useful in thatlate flowering is desirable in crops where the vegetative portion of theplant is harvested (often vegetative growth stops when plants make thetransition to flowering). In this case, it can be advantageous toprevent or delay flowering in order to increase yield. Also, preventionof flowering can be useful in these same crops in order to prevent thespread of transgenic pollen and/or to prevent seed set. In any case, theoverexpressors were clearly smaller, an undesirable phenotype which hasto be corrected before overexpression of the gene can lead to any usefulcrop product.

G362 (SEQ ID NO: 65)

Published Information

G362 was identified in the sequence of BAC clone T10024, GenBankaccession number AC007067, released by the Arabidopsis GenomeInitiative.

Experimental Observations

The function of this gene was analyzed using transgenic plants in whichG362 was expressed under the control of the 35S promoter. 35S::G362 hada number of developmental effects with the most prominent result beingan increase in trichome number as well as the ectopic formation oftrichomes. Overexpression of G362 also increased anthocyanin levels invarious tissues at different stages of growth. Seedlings sometimesshowed high levels of pigment in the first true leaves. Late floweringlines also became darkly pigmented. Seeds from a number of lines wereobserved to develop patches of dark purple pigmentation. Inflorescencesfrom 35S::G362 plants were thin, and flowers sometimes displayed poorlydeveloped organs. The seed yield from many lines was somewhat poor.35S::G362 transgenic plants showed no phenotypic alterations in responseto the physiological or biochemical analyses performed.

As determined by RT-PCR, G362 was expressed in roots, and was expressedat significantly lower levels in siliques, seedlings, and shoots. Noexpression of G362 was detected in the other tissues tested. G362expression was induced in rosette leaves by heat stress.

Potential Applications

G362 or its equivalogs can be used to alter anthocyanin production. Theutilities of this gene includes alterations in pigment production forhorticultural purposes, and possibly increasing stress resistance incombination with another transcription factor.

G362 or its equivalogs can be used to delay flowering in transgenicplants. This can have useful implications in crop plants. In speciessuch as sugarbeet where the vegetative parts of the plants constitutethe crop and the reproductive tissues were discarded, it would beadvantageous to delay or prevent flowering. In addition, extendingvegetative development could have a beneficial effect on yield, sincethe plants have a longer time to build up their photosynthetic capacity.This in turn can translate into larger accumulations of storageproducts.

G362 or its equivalogs can be used to alter trichome number anddistribution in plants. Trichome glands on the surface of many higherplants produce and secrete exudates, which give protection from theelements and pests such as insects, microbes and herbivores. Theseexudates may physically immobilize insects and spores, may beinsecticidal or anti-microbial or they may allergens or irritants toprotect against herbivores. Trichome have also been suggested todecrease transpiration by decreasing leaf surface air flow, and byexuding chemicals that protect the leaf from the sun.

Another utility for G362 or its equivalogs is to increase the density ofcotton fibers in cotton bolls. Cotton fibers are modified unicellulartrichomes that are produced from the ovule epidermis. Typically only 30%of the epidermal cells take on a trichome fate (Basra et al. (1984) Int.Rev. Cytol. 89:65-113). Thus cotton yields might be increased byinducing a greater proportion of the ovule epidermal cells to becomefibers.

Depending on the plant species, varying amounts of diverse secondarybiochemicals (often lipophilic terpenes) are produced and exuded orvolatilized by trichomes. These exotic secondary biochemicals, which arerelatively easy to extract because they are on the surface of the leaf,have been widely used in such products as flavors and aromas, drugs,pesticides, and cosmetics. One class of secondary metabolites, thediterpenes, can effect several biological systems such as tumorprogression, prostaglandin synthesis, and tissue inflammation. Inaddition, diterpenes can act as insect pheromones, termite allomones,and can exhibit neurotoxic, cytotoxic and antimitotic activities. As aresult of this functional diversity, diterpenes have been the target ofresearch several pharmaceutical ventures. In most cases where themetabolic pathways were impossible to engineer, increasing trichomedensity or size on leaves may be the only way to increase plantproductivity.

Thus, the use of G362 and its homologs to increase trichome density,size, or type may therefore have profound utilities in so calledmolecular farming practices (for example, the use of trichomes as amanufacturing system for complex secondary metabolites), and inproducing insect-resistant and herbivore-resistant plants.

G371 (SEQ ID NO: 67)

Published Information

G371 was identified as the published gene A-RZF accession number U81598,expressed preferentially in seed development (Zou et al. (1997) Gene196:291-295).

Experimental Observations

The sequence of G371 was experimentally determined and the function ofG371 was analyzed using transgenic plants in which G371 was expressedunder the control of the 35S promoter. Plants overexpressing G371appeared to be more sensitive to Botrytis infection. No alteredmorphological or biochemical phenotypes were observed for G371overexpressing plants.

The function of this gene was also studied by knockout analysis. Thephenotype of the G371 knockout was wild-type for all assays performed.

Array analysis of endogenous levels of G371 indicated that this gene wasexpressed predominantly in embryos, consistent with its publishedexpression pattern. There was no change in the expression of this genein response to environmental stress according to RT-PCR data. However,according to array data, this gene was induced 4-fold in response toErysiphe infection, its expression was not affected by infection withFusarium, and it was repressed 3-fold after a 12 hour treatment at 4° C.

Potential Applications

Because G371 confers sensitivity to Botrytis, this gene or itsequivalogs has utility in producing pathogen resistant plants.

G390 (SEQ ID NO: 69)

Published Information

G390 was isolated by Ruzza et al. (GenBank Accession: CAD29544,gi:20069421) using degenerate oligonucleotides corresponding to aconserved 6 amino acid sequence from the helix-3 region of athb-1 andathb-2. It was named athb-9. The published Northern blot showed slightlyhigher level of expression in stems, and lower levels in leaves,flowers, roots, and siliques. The G390 protein shares very extensiveamino acid identity with other HD-ZIP class III proteins that exist inArabidopsis (for example, G391 and G438). HD-ZIP class III proteins areknown to have complex roles in determining meristem development,vascular tissue formation, and stem lignification (Baima et al. (1995)Development 12:4171-4182; Baima et al. (2001) Plant Physiol.126:643-655; Talbert et al. (1995) Development 121:2723-2735; Thong etal. (1997) Plant Cell 9:2159-2170; Sessa et al. (1998) Plant Mol. Biol.38:609-622; Zhong et al. (1999) Plant Cell 11:2139-2152; Ratcliffe etal. (2000) Plant Cell 12:315-317; and Otsuga et al. (2001) Plant J.25:223-236).

Experimental Observations

Fourteen 35S::G390 T1 lines were obtained which displayed a consistentmorphological phenotype; the majority of these plants were slightlysmall, had abnormal phyllotaxy, and exhibited stem bifurcations in whichshoot meristems split to form two or three separate shoots.Additionally, a significant number of these extra T1 lines floweredearlier than controls. Comparable effects were obtained byoverexpression of G391.

Potential Applications

The overexpression data suggest that G390 or its equivalogs has utilityin the manipulation of shoot architecture. Additionally, since a numberof the 35S::G390 lines flowered early, this gene or its equivalogs canbe used to manipulate flowering time.

G391 (SEQ ID NO: 71)

Published Information

G391, also known as Athb-14, was isolated based on its homology with apreviously identified homeobox containing gene, Athb-8 (G392). Thefull-length cDNAs encode proteins of 852 amino acids. Athb-8, -9 and -14(G392, G390, and G391, respectively) are members of a small family ofHD-Zip proteins (HD-ZIP III) characterized by a HD-Zip motif confined tothe N-terminus of the polypeptide. The spatial organization of theHD-Zip domain of Athb-8, -9 and -14 is different from that of the Athb-1(G409, a member of the HD-ZIP I family) and Athb-2 (G400, a member ofthe HD-ZIP II family) HD-Zip domains. DNA binding analysis performedwith random-sequence DNA templates showed that the Athb-9 HD-Zip(HD-Zip-9) domain, but not the Athb-9 HD alone, binds to DNA. TheHD-Zip-9 domain recognizes a 11 bp pseudopalindromic sequence(GTAAT(G/C)ATTAC) as determined by selecting high-affinity binding sitesfrom random-sequence DNA. Moreover, gel retardation assays demonstratedthat the HD-Zip-9 domain binds to DNA as a dimer. These data supportedthe notion that the HD-ZIP III domain interacts with DNA recognitionelements in a fashion similar to the HD-ZIP I and II domains.

The G391 protein shares very extensive amino acid identity with otherHD-ZIP class III proteins that exist in Arabidopsis (for example, G390and G438). These genes are known to have complex roles in determiningmeristem development, vascular tissue formation, and stem lignification(Baima et al. (1995) Development 12:4171-4182; Baima et al. (2001) PlantPhysiol. 126:643-655; Talbert et al. (1995) Development 121:2723-2735;Thong et al. (1997) Plant Cell 9:2159-2170; Sessa et al. (1998) PlantMol. Biol. 38:609-622; Zhong et al. (1999) Plant Cell 11:2139-2152;Ratcliffe et al. (2000) Plant Cell 12:315-317; and Otsuga et al. (2001)Plant J. 25:223-236).

Experimental Observations

The function of this gene was analyzed using transgenic plants in whichG391 was expressed under the control of the 35S promoter. Althoughplants from the T2 generation were wild-type in morphology, the T1plants showed significant deleterious effects. The plants were small anddark green with short bolts. All other phenotypes were wild-type in allassays performed. As determined by RT-PCR, G391 was moderately expressedin shoots, and was expressed at lower levels in roots, flowers, androsettes.

An additional sixteen 35S::G391 T1 lines were obtained that displayed aconsistent morphological phenotype; the majority of these plants weresmall, had abnormal phyllotaxy, and exhibited stem bifurcations in whichshoot meristems split to form two or three separate shoots. Additionallya significant number of these extra T1 lines flowered earlier thancontrols. Comparable effects were obtained by overexpression of G390.

Potential Applications

The overexpression data suggested that G391 or its equivalogs haveutility in the manipulation of shoot architecture. Additionally, since anumber of the 35S::G391 lines flowered early, this gene or itsequivalogs can be used to manipulate flowering time.

G409 (SEQ ID NO: 73)

Published Information

G409, also named Athb-1, was one of the earliest plant homeodomainleucine (HD-ZIP) zipper genes cloned. It was isolated from a cDNAlibrary by highly degenerate oligonucleotides corresponding to aconserved eight amino acid sequence from the helix-3 region of thehomeodomain. The protein was found to transactivate a promoter linked toa specific DNA binding site (CAATTATTG) by transient expression assays.Overexpression of Athb-1 affected the development of palisade parenchymaunder normal growth conditions, resulting in light green sectors inleaves and cotyledons, whereas other organs in the transgenic plantsremained normal.

Experimental Observations

G409 was induced by drought and repressed by NaCl. Plants overexpressingG409 were more tolerant to infection by the fungal pathogen Erysipheorontii. In addition to the Erysiphe tolerant phenotype, theoverexpressors were slightly early flowering.

Potential Applications

The expression of transcription factors such as G409 or its equivalogsinvolved in plant/pathogen interaction can be modulated to manipulatethe plant defense- wound- or insect-response in order to generatepathogen resistant plants.

G427 (SEQ ID NO: 75)

Published Information

G427 corresponds to KNAT4, one of four KNOX class II homeobox genes inArabidopsis. This gene was originally identified by Serikawa et al.((1996) Plant Mol. Biol. 32:673-683) using low-stringency screening ofArabidopsis cDNA libraries using the kn1 homeobox from maize No geneticcharacterization of KNAT4 have been published, but it is expressed athigh levels in leaves and young siliques (Serikawa et al. (1996) supra).It should be noted that KNAT4 shares a very high level of sequencesimilarity with another KNOX class II gene, KNAT3 (G426). Expression ofeach of these genes is light dependent, suggesting that that they mighthave a role in light regulated developmental processes (Serikawa et al.(1996) supra; Serikawa et al. (1997) Plant J. 11:853-861).

Experimental Observations

The function of G427 was assessed by analysis of transgenic Arabidopsislines in which the cDNA was constitutively expressed under the controlof the 35S CaMV promoter.

35S::G427 transformants flowered markedly earlier than wild-typecontrols in conditions of either continuous light or a 12-hourphotoperiod. Such results indicated that G427 can promote flowering inArabidopsis under either inductive or non-inductive conditions. Thesedata correlated well with the published observation that G427 expressionis light regulated, and indicated that the gene likely has a function inthe regulation of flowering time in Arabidopsis. Additionally, 35S::G427seedlings were noted to have rather vertically positioned leaves, afeature that is often apparent in plants with abnormal light regulateddevelopment.

Overexpression of G427 in Arabidopsis also resulted in an increase inseed oil and a decrease in seed protein in two T2 lines. No otherphenotypic alterations were observed.

Potential Applications

G427 or its equivalogs can be used to manipulate seed oil and seedprotein content, which may be of nutritional value for humanconsumption, and for animal feeds.

G427 or its equivalogs can be used to regulate flowering time incommercial species. A wide range of potential applications exist;prevention of flowering might help maximize vegetative yields andprevent escape of GMO pollen, whereas accelerating flowering couldshorten crop and tree breeding programs.

Additionally, G427 or its equivalogs can be used in inducible systemsthat could be used to synchronize flowering in a crop.

G438 (SEQ ID NO: 77)

Published Information

G438 was identified as a homeobox gene (MUP 24.4) within P1 clone MUP 24(GenBank accession number AB005246). G438 was identified as theArabidopsis REVOLUTA (REV) gene (Ratcliffe et al. (2000) Plant Cell12:315-317). Based on its mutant phenotype, REV had previously beenidentified as having a key role in regulating the relative growth ofapical versus non-apical (cambial) meristems (Alvarez (1994) inArabidopsis: An Atlas of Morphology and Development (ed. J. Bowman), pp.188-189, New York, N.Y.: Springer-Verlag; Talbert et al. (1995)Development 121:2723-2735). The revoluta phenotype was highlypleiotropic but was characterized by a failure in development of alltypes of apical meristem: lateral shoot meristems in the axils ofcauline and rosette leaves were often completely absent, or replaced bya solitary leaf. These effects were most evident in higher order shoots,but in some cases, the primary shoot meristem also failed and terminatedgrowth in a cluster of filamentous structures. Rev floral meristemsoften failed to complete normal development and form incomplete orabortive filamentous structures. In contrast to apical meristems,structures formed by non-apical meristems, such as leaves, stems, andfloral organs often became abnormally large and contorted in the revmutant.

The features of rev mutants were similar to those of the interfascicularfiberless1 (ifl1) mutant. Ifl1 was isolated during screens for mutantslacking normal stem fiber differentiation (Thong et al. (1997) PlantCell 9:2159-2170). Wild-type Arabidopsis plants form interfascicularfibers which became lignified and added support to the inflorescencestem (Aloni (1987) Annu. Rev. Plant Physiol. 38:179-204); Zhong et al.(1997) supra; Zhong et al. (1999) Plant Cell 11:2139-2152). In the ifl1mutant, normal interfascicular fibers were absent and thedifferentiation of both xylary fibers and vessel elements was disrupted.In addition to these internal features, ifl1 mutants had secondarymorphological features very similar to those of rev. Recently the IFL1gene was cloned by Zhong et al. (1999 supra). It was found that the IFL1sequence and map position were identical to those of the REV genecloned, demonstrating that REV and IFL1 are the same gene. (Ratcliffe etal. (2000) supra).

It had been suggested that REV promotes the growth of apical meristems(including floral meristems) at the expense of non-apical meristems(Talbert et al. (1995) supra). It is not yet clear, however, whetherexpression data support such a role: strong expression of REV has beendetected in interfascicular regions and developing vascular tissue, butin-situ expression analysis of apical meristems has not yet beenreported. (Thong et al. (1999) supra). REV is a group III HD-ZIP proteinand shares high sequence similarity (and organization) with the proteinsencoded by three other Arabidopsis genes: Athb8, Athb9, and Athb14(Sessa et al. (1998) Plant Mol. Biol. 38:609-622). It is possible,therefore, that these genes act together in the same developmentalprocess. Supporting this suggestion, Athb8 had a similar expressionpattern to REV and was transcribed in the procambial regions of vascularbundles (Baima et al. (1995) Development 12:4171-4182).

Experimental Observations (Knockout)

G438 was initially identified as MUP24.4, a novel putative homeobox genewithin P1 clone MUP24 (GenBank Accession AB005246). Annotation wasconfirmed by isolation of the G438 cDNA: the cDNA had an in-frame stopcodon immediately 5′ to the predicted start codon and comprised 18 exonsthat had been predicted within the genomic sequence.

Plants homozygous for a T-DNA insertion in the G438 sequence wereobtained by PCR based screening of DNA pools from the Jack Collection ofinsertional mutants (Campisi et al. (1999) Plant Journal 17:699-707).The T-DNA insertion was located 466 bp downstream of the putative startcodon, and was predicted to create a null mutation. The mutation wasrecessive and produced a revoluta phenotype. Complementation crosses andsequencing of a known revoluta allele demonstrated that G438 wasREVOLUTA.

RT-PCR analyses detected G438 expression at medium to high levels in alltissues and conditions tested. Further expression analysis was possiblesince the T-DNA insertion contained an enhancer trap construct (Campisiet al. (1999) supra). GUS staining could therefore be used to reveal theexpression pattern of genes within which insertions occurred. GUSstaining of seedlings homozygous and heterozygous for the G438 T-DNAinsertion revealed very strong expression within axillary shoots. Thisexpression data correlates with the marked effects of the rev mutationon outgrowth of higher order shoots.

Experimental Observations (Overexpressor)

A full-length clone was amplified from cDNA derived from mixed tissuesamples, and 35S::G438 transformants were generated. These linesappeared wild-type in the physiological assays, but showed differencesin morphology compared with control plants. At early stages, a smallnumber of T1 plants displayed aberrant phyllotaxy and were ratherdwarfed, but these effects were inconsistent, and the majority of linesappeared wild-type. At later stages, however, around half of the primarytransformants, from two of the three T1 sowings, developed slightlylarger flatter leaves than wild type at late stages. The progeny of fourlines that had shown these phenotypes were examined in the T2generation. At late stages, plants from two of these T2 populationsagain displayed slightly broad flat leaves, but plants from the othertwo T2 populations appeared wild-type at all stages.

A single T1 plant line out of a total of 37 lines had highly aberrantshoot meristem development. At the early seedling stage, it appeared asthough the primary shoot apex of this individual had developed into aterminal leaf-like structure. Subsequent growth then continued from anaxillary shoot meristem that initiated from the base of a cotyledonpetiole. However, this effect became silenced between generations andwas not observed in the T2 progeny from one line. Given that this effectwas observed in only a single line, it could have been the result of anactivation tagged locus at the T-DNA insertion site, rather than due toG438 expression. However, the phenotype would fit with a role for REV inregulating apical meristem development.

Potential Applications

The mutant phenotypes indicated that REV/IFL1 or its equivalogs have animportant role in determining overall plant architecture and thedistribution of lignified fiber cells within the stem. A number ofutilities can be envisaged based upon these functions.

Modifying the activity of REVOLUTA orthologs from tree species can offerthe potential for modulating lignin content. This can allow the qualityof wood used for furniture or construction to be improved. Lignin isenergy rich; increasing lignin composition could therefore be valuablein raising the energy content of wood used for fuel. Conversely, thepulp and paper industries seek wood with a reduced lignin content.Currently, lignin must be removed in a costly process that involves theuse of many polluting chemicals. Consequently, lignin is a seriousbarrier to efficient pulp and paper production (Tzira et al. (1998)TIBTECH 16:439-446; Robinson (1999) Nature Biotechnology 17:27-30). Inaddition to forest biotechnology applications, changing lignin contentmight increase the palatability of various fruits and vegetables.

In Arabidopsis, reduced REV activity results in a reduction ofhigher-order shoot development. Reducing activity of REV orthologs maygenerate trees that lack side branches, and have fewer knots in thewood. Altering branching patterns can also have applications amongstornamental and agricultural crops. For example, applications might existin any species where secondary shoots currently have to be removedmanually, or where changes in branching pattern could increase yield orfacilitate more efficient harvesting.

G450 (SEQ ID NO: 79)

Published Information

G450 is IAA14, a member of the Aux/IAA class of small, short-livednuclear proteins that contain four conserved domains. IAA14 was found asone of a group of Arabidopsis IAA genes that was isolated based onhomology to early auxin-induced genes of pea (Abel et al. (1995) J. Mol.Biol. 251:533-549). Recently a gain-of-function mutant in IAA14, slr(solitary root), was found to abolish lateral root formation, reduceroot hair formation, and impair gravitropic responses (Fukaki (2001)Abstracts 12th Intl. Conf. Arabidopsis Res. #448, Madison, Wis.).

Experimental Observations

Overexpression of G450 influenced leaf development, overall plantstature, and seed size. 35S::G450 plants produced seeds that were largerthan wild-type seed.

Potential Applications

G450 or its equivalogs can used to produce larger seed in plants, whichmay positively influence seed storage characteristics, appearance andyield.

G464 (SEQ ID NO: 81)

Published Information

G464 is IAA12, a member of the Aux/IAA class of small, short-livednuclear proteins that contain four conserved domains. IAA12 was found asone of a group of Arabidopsis IAA genes that was isolated based onhomology to early auxin-induced genes of pea. IAA12 transcripts weremodestly (2 to 4-fold) induced by auxin, with optimal induction at 10 μMauxin (Abel et al. (1995) J. Mol. Biol. 251:533-549).

Experimental Observations

G464 overexpressing Arabidopsis lines showed enhanced germination inhigh heat conditions. In addition, one Arabidopsis line overexpressingG464 showed an increase in total seed protein and a decrease in totalseed oil by NIR in one assay.

Potential Applications

G464 or its equivalogs in native or altered form is useful to produceplants that germinate better in hot conditions.

G470 (SEQ ID NO: 83)

Published Information

A partial cDNA clone corresponding to G470 was isolated in a two-hybridscreen for proteins that interact with ARF1, a transcription factor thatbinds to auxin response elements, and this clone was named ARF1 BindingProtein (Ulmasov et al. (1997) Science 276:1865-1868). A full-lengthclone was later isolated, and the gene was renamed ARF2 (Ulmasov et al.(1999a) Proc. Natl. Acad. Sci. 96:5844-5849). ARF2 was shown to bind toan auxin response element (Ulmasov et al. (1999b) Plant J. 19:309-319).

Co-transfection of ARF2 and a reporter construct with an auxin responseelement into carrot protoplasts did not result in either activation orrepression of transcription of the reporter gene (Ulmasov et al. (1999a)supra). ARF2 binding to palindromic auxin response elements is thoughtto be facilitated by dimerization mediated by the carboxy-terminaldomain of ARF2 (Ulmasov et al. (1999b) supra). It is possible that ARF2regulates gene expression through heterodimerization with other ARFproteins or with IAA proteins. ARF2 was found to be expressed uniformlyin roots, rosette leaves, cauline leaves, flowers, and siliques (Ulmasovet al. (1999b) supra).

Experimental Observations

Expression of a truncated G470 clone in the antisense orientation underthe 35S promoter caused infertility in Arabidopsis. In primarytransformants expressing the G470 clone, the stamens failed to elongateproperly. Pollen was produced, but was not deposited on the stigma. Thetransformants appeared otherwise morphologically normal. Because of theinfertility of the primary transformants, no material was available forbiochemical and physiological analyses. The truncated clone correspondsto the carboxy-terminal portion of the ARF2 protein, and lacks the DNAbinding domain.

Potential Applications

G470 or its equivalogs are useful in engineering infertility inself-pollinating plants.

G477 (SEQ ID NO: 85)

Published Information

G477 corresponds to SPL6 (AJ011643, Cardon et al. (1999) Gene237:91-104), a member of the SBP family of transcription factors. G477is expressed constitutively throughout the development of Arabidopsis.Outside the SBP-domain, G477 has a putative myc-like helix-loop-helixdimerization domain (Cardon et al. (1999) supra).

Experimental Observations

The complete sequence of G477 was determined. The function of this genewas analyzed using transgenic plants in which G477 was expressed underthe control of the 35S promoter. The phenotype of these transgenicplants was wild-type in all morphological and biochemical assaysperformed.

Plants overexpressing G477 were slightly more sensitive to theherbicides glyphosate and acifluorfen and to oxidative stress caused byrose bengal compared with wild-type controls. Plants overexpressing G477also develop more disease symptoms following inoculation with a moderatedose of Sclerotinia sclerotiorum compared with control plants. It iswell known that oxidative stress is a component of a plant defenseresponse to pathogen and therefore the disease susceptibility phenotypecould be related to a general sensitivity to oxidative stress.

G477 was expressed in all tissues and under all conditions tested inRT-PCR and cDNA micro array experiments.

Potential Applications

G477 activity was shown to affect the response of transgenic plants tothe fungal pathogen Sclerotinia sclerotiorum and oxidative stresstolerance. Therefore, G477 or its equivalogs can be used to manipulatethe defense response in order to generate pathogen-resistant plants.

G481 (SEQ ID NO: 87)

Published Information

G481 is equivalent to AtHAP3a which was identified by Edwards et al.((1998) Plant Physiol. 117:1015-1022) as an EST with extensive sequencehomology to the yeast HAP3. Northern blot data from five differenttissue samples indicated that G481 was primarily expressed in flowerand/or silique, and root tissue.

Experimental Observations

G481 was analyzed through its ectopic overexpression in plants. G481overexpressors were more tolerant to high sucrose in a germinationassay. The phenotype of G481 was mild; however, there was a consistentdifference in the hypocotyl and root elongation in the overexpressorplants compared to wild-type controls. Sucrose-sensing has beenimplicated in the regulation of source-sink relationships in plants.Consistent with the sugar sensing phenotype of the G481 overexpressorswere the results from the biochemical analysis of G481 overexpressorplants indicating that one line had higher amounts of seed oils andlower amounts of seed protein. This suggested that G481 was involved inthe allocation of storage compounds to the seed. One G481 overexpressorline was darker green in the T2 generation, which could mean a higherphotosynthetic rate consistent with the possible role of G481 in sugarsensing.

G481 overexpressing plants were found to be more tolerant to drought ina soil-based assay.

Potential Applications

The utility of G481 or its equivalogs includes a role in sugar sensing,a plant mechanism that has been shown to be involved in thefollowing: 1) altering storage compound accumulation (oil and/orprotein) in seeds which could impact yield and seed quality, and 2)altering photosynthetic rate which could also impact yield in vegetativetissues as well as seed. G481 was shown to alter sugar sensing. Sugarsare key regulatory molecules that affect diverse processes in higherplants including germination, growth, flowering, senescence, sugarmetabolism and photosynthesis. Sucrose is the major transport form ofphotosynthate and its flux through cells has been shown to affect geneexpression and alter storage compound accumulation in seeds (source-sinkrelationships).

The enhanced germination phenotype of transgenic plants overexpressingG481 under a condition of drought or osmotic stress (such as highconcentrations of sucrose) suggested the gene or its equivalogs can alsobe used to improve plant tolerance to water deficit related conditionssuch as water deprivation, salt stress, and freezing stress. Thus, G481can be used to engineer plants with enhanced stress tolerance that canultimately impact survivability and yield.

G482 (SEQ ID NO: 89)

Published Information

G482 is equivalent to AtHAP3b which was identified by Edwards et al.((1998) Plant Physiol. 117:1015-1022) as an EST with homology to theyeast gene HAP3b. Edwards' northern blot data suggests that AtHAP3b isexpressed primarily in roots. No other functional information regardingG482 is publicly available.

Experimental Observations

G482 function was analyzed through its ectopic overexpression in plantsunder the control of a 35S promoter. G482 overexpressors were moretolerant to high NaCl in a germination assay.

RT-PCR analysis of endogenous levels of G482 transcripts indicated thatthis gene was expressed constitutively in all tissues tested. A cDNAarray experiment supported the RT-PCR derived tissue distribution data.G482 was not induced above basal levels in response to any environmentalstress treatments tested.

Potential Applications

The utilities of this gene or its equivalogs include the ability toconfer salt tolerance during the germination stage of a crop plant. Thiswould most likely impact survivability and yield. Evaporation of waterfrom the soil surface causes upward water movement and salt accumulationin the upper soil layer, where the seeds were placed. Thus, germinationnormally takes place at a salt concentration much higher than the meansalt concentration in the whole soil profile.

G484 (SEQ ID NO: 91)

Published Information

G484 is equivalent to ATHDR1B and was isolated by Kuromori et al.((1994) Nucleic Acids Res. 22:5296-5301). The Arabidopsis sequence ishighly homologous to the human DR1 gene that has been shown to interactwith TATA-binding protein (TBP) to repress transcription of class IIgenes (Yeung et al. (1994) Genes Dev. 8:2097-2109).

Experimental Observations

Homozygous knockout mutant plants as well as plants ectopicallyoverexpressing G484 were used to determine the function of this gene inArabidopsis. Insertion of T-DNA into G484 at nucleotide position +439with respect to the start ATG codon was within the first third of theG484 coding sequence of the gene and therefore was likely to result in anull mutation. The phenotype for G484 overexpressor and knockout mutantplants was similar to wild-type for all morphological, biochemical andphysiological assays performed. RT-PCR analysis of the endogenous levelsof G484 transcripts indicated that this gene was expressed primarily inshoots, roots and flowers, with a low level expression in the othertissues tested. G484 was not induced significantly above basal levels inresponse to any environment stress treatments tested.

Potential Applications

G484 knockout mutant seed had an altered glucosinolate profile andtherefore the gene or its equivalogs can be used to modify glucosinolatecomposition in plants.

G489 (SEQ ID NO: 93)

Published Information

G489 was identified from a BAC sequence that showed high sequencehomology to AtHAP5-like transcription factors in Arabidopsis. Nopublished information is available regarding the function of this gene.

Experimental Observations

The function of G489 was analyzed through its ectopic overexpression inplants. G489 overexpressors were more tolerant to high NaCl stress,showing more root growth and leaf expansion compared with the controlsin culture. Two well characterized ways in which NaCl toxicity ismanifested in the plant is through general osmotic stress and potassiumdeficiency due to the inhibition of its transport. These G489overexpressor lines were more tolerant to osmotic stress in general,showing more root growth on mannitol containing media.

RT-PCR analysis of endogenous levels of G489 transcripts indicated thatthis gene was expressed constitutively in all tissues tested. A cDNAarray experiment confirmed the RT-PCR derived tissue distribution data.G489 was not induced above basal levels in response to the stresstreatments tested.

Potential Applications

The utilities of this gene or its equivalogs include the ability toconfer salt tolerance during the growth and developmental stages of acrop plant. This would impact yield and or biomass.

G490 (SEQ ID NO: 95)

Published Information

G490 is member of the Hap5-like subfamily of the CAAT-box bindingtranscription factors. G490 was identified in the sequence of BAC MXA21,GenBank accession number AB005247, released by the Arabidopsis GenomeInitiative.

Experimental Observations

The complete sequence of G490 was determined. The function of this genewas analyzed using transgenic plants in which G490 was expressed underthe control of the 35S promoter. The phenotype of these transgenicplants was wild-type in all physiological assays performed.Overexpression of G490 resulted in a marked early flowering phenotypeunder continuous light conditions.

During initial studies on lines #1-20, plants were not carefullyexamined for flowering time, and at later developmental stages, appearedto have a wild-type phenotype. To assess flowering time more carefully,a further batch of 35S::G490 T1 plants were grown. The majority of theseplants showed a very clear acceleration of flowering and had visibleflower buds up to a week earlier than wild type. At later stages theplants appeared wild-type. To confirm these observations, T2 progenyfrom three early flowering T1 plants were grown; all three T2 linesshowed early flowering.

In addition to the flowering time phenotype, seed of 35S::G490transgenic plants showed altered tocopherol composition. In seeds of twolines, an increase in the percentage of delta-tocopherol was observed.

As determined by RT-PCR, G490 was expressed at low levels in flower,rosette leaf, embryo and silique. No expression of G490 was detected inthe other tissues tested. G490 expression is induced to low levels inrosette leaves by auxin treatment, drought, heat, osmotic and saltstress treatments.

Potential Applications

One utility of a gene such as G490 or its equivalogs is to accelerateflowering.

In addition, G490 or its equivalogs can be used to alter tocopherolcomposition. Tocopherols have anti-oxidant and vitamin E activity.

G504 (SEQ ID NO: 97)

Published Information

G504 was identified in the sequence of BAC F11P17, GenBank accessionnumber AC002294, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G504 was determined. The function of this genewas analyzed using transgenic plants in which G504 was expressed underthe control of the 35S promoter. The phenotype of these transgenicplants was wild-type in all physiological and biochemical assaysperformed. 35S::G504 transgenic plants had a subtle leaf phenotype inthe early developmental stages but were wild-type in appearance in laterstages of development. In one transgenic line, a decrease in seed oil asmeasured by NIR was observed. Also, seeds of this same line also showedan increase in the percentage of 18:2 fatty acid and a decrease in thepercentage of 20:1 fatty acid.

In an RT PCR experiment, endogenous G504 appeared to be expressedspecifically and at high levels in flower tissue. No induction ofendogenous G504 expression in leaf tissue was detected in response toany environmental conditions tested.

Potential Applications

G504 or its equivalogs may be used to modify seed oil content in seeds,which may be very important for the nutritional value and production ofvarious food products.

The promoter of G504 can be used to engineer flower specific geneexpression.

G509 (SEQ ID NO: 99)

Published Information

G509 was identified in the sequence of BAC F2009, GenBank accessionnumber AL021749, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G509 was analyzed using transgenic plants in which G509was expressed under the control of the 35S promoter, as well as using aline homozygous for a T-DNA insertion in G509. The T-DNA insertion ofG509 at nucleotide position +1583 with respect to the start ATG codonwas approximately half way into the coding sequence of the gene andtherefore was likely to result in a null mutation. G509 primarytransformants showed no significant morphological differences fromcontrol plants, though one T2 line was noted to be small and sickly atthe seedling and rosette stages, and pale and late flowering at theflowering stage. Knockout plants showed no consistent morphologicaldifferences from controls. G509 knockout plants may be more susceptibleto infection with a moderate dose of the fungal pathogen Erysipheorontii; 8 out of 8 plants tested showed more fungal growth comparedwith the wild-type controls. G509 lines had significantly higher levelsof chlorophyll a, and lower levels of chlorophyll b in seeds.

G509 knockout mutants produced more seed oil and more seed protein thanwild-type control plants.

Endogenous G509 was expressed constitutively in all tissues tested, withthe highest levels of expression in shoots, roots, flowers and siliques.

Potential Applications

G509 or its equivalogs can be used to produce plants with altered seedoil and seed protein content.

G509 or its equivalogs can be used to manipulate the defense response inorder to generate pathogen-resistant plants.

In addition, G509 or its equivalogs can be used to regulate the levelsof chlorophyll in seeds.

G519 (SEQ ID NO: 101)

Published Information

G519 was first identified in the sequence of the P1 clone MBK5, GenBankaccession number AB005234, released by the Arabidopsis GenomeInitiative.

Closely Related Genes from Other Species

A related gene to G519 is the rice gene OsNAC6 (GenBank accession numberBAA89800).

Experimental Observations

The function of G519 was analyzed with transgenic plants in which G519was expressed under the control of the 35S promoter.

RT-PCR analysis was used to determine the endogenous levels of G519 in avariety of tissues and under a variety of environmental stress-relatedconditions. G519 was constitutively expressed with the highest level ofexpression in shoots, roots and seedlings. RT-PCR data also indicated aninduction of G519 transcripts accumulation upon auxin, abscisic acid(ABA), cold, heat, Fusarium and salicylic acid (SA) treatments.

As measured by NIR, G519 overexpressors were found to have increasedseed oil content compared to wild-type plants.

Potential Applications

G519 or its equivalogs may be used to alter seed oil content in plants,which may be very important for the nutritional value and production ofvarious food products.

G545 (SEQ ID NO: 103)

Published Information

G545 was discovered independently by two groups. Lippuner et al. (1996)J. Biol. Chem. 271:12859-12866) identified G545 as an Arabidopsis cDNA(STZ), which increases the tolerance of yeast to Li+ and Na+. They foundthat STZ expression is most abundant in leaves and roots, and that itslevel of expression increases slightly upon exposure of the plant tosalt. The second group (Meissner et al (1997) Plant Mol. Biol.33:615-624), identified G545 (ZAT10) in a group of Arabidopsis C2H2 zincfinger protein-encoding cDNAs that they isolated by degenerate PCR.According to their data, ZAT10 is expressed in roots, shoots and stems.

Closely Related Genes from Other Species

A closely related non-Arabidopsis sequence is a cDNA from thenitrogen-fixing species Datisca glomerata (AF119050). The similarity ofthis sequence with G545 extends beyond the conserved domain.

Experimental Observations

Plants overexpressing G545 flowered early, and in extreme cases wereinfertile. G545 overexpression conferred tolerance of transgenic plantsto phosphate deficiency. This could be the result of insensitivity tophosphate, higher rates of phosphate assimilation or larger stores ofphosphate. G545 overexpressors also appeared to be more sensitive toNaCl than wild-type plants. This result was unexpected, since yeastcells overexpressing G545 are more tolerant to salt stress than controlcells. There may be a dominant negative effect in plants, triggered bythe over-accumulation of the G545 protein, which does not exist inyeast.

G545 overexpressing plants appeared to be significantly more susceptibleto pathogens than control plants. This implied a role for the G545 inthe control of defense mechanisms.

Potential Applications

G545 or equivalog overexpression may result in tolerance to phosphatedeficiency. Young plants have a rapid intake of phosphorous, so it isimportant that seed beds have high enough content in phosphate tosustain their growth. Also, root crops such as carrot, potato andparsnip will all decrease in yield if there is insufficient phosphateavailable. Phosphate costs represent a relatively small but significantportion of farmers' operating costs (3-4% of total costs to a cornfarmer in the US, higher to a vegetable grower). Plants that aretolerant to phosphate deficiency can represent a cost saving forfarmers, especially in areas where soils are very poor in phosphate.

Another desirable phenotype, salt tolerance, may arise from G545 orequivalog silencing rather than overexpression. Additionally, G545appeared to be induced by cold, drought, salt and osmotic stresses,which was in agreement with a potential role of the genes in protectingthe plant in such adverse environmental conditions.

G545 also appears to be involved in the control of defense processes.However, overexpression of G545 made Arabidopsis plants more susceptibleto disease. This negative effect will have to be corrected before G545can be used in a crop to induce tolerance to low phosphate, such as byrestricting overexpression of G545 or its equivalogs to roots.

G546 (SEQ ID NO: 105)

Published Information

G546 was identified in the sequence of P1 clone MJB20 and BAC cloneT19E12, GenBank accession number AC007584, released by the ArabidopsisGenome Initiative.

Closely Related Genes from Other Species

G546 homologs in other species are Y14573.1:33104 . . . 33991 frombarley, OSJNBb0064P21.7 from rice.

Experimental Observations

RT-PCR was used to analyze the endogenous levels of G546 transcripts.RT-PCR data indicated that G546 was expressed constitutively in alltissues examined. There was a moderate level of G546 transcript detectedin shoots and roots while in flowers, rosette and cauline leaves, andsiliques transcript level was low. G546 transcripts were not elevated inresponse to the environmental stress treatments.

The function of this gene was analyzed using transgenic plants in whichG546 was expressed under the control of the 35S promoter. Overexpressionof G546 in Arabidopsis resulted in one line in which seedlings were ABAinsensitive in a germination assay. Morphologically, the plants weresmall at early stages, grew slowly, became dark colored, and senescedlate. Somewhat similar effects were observed in approximately half ofthe primary transformants. 35S::G546 transformants also sporadicallydisplayed increased anthocyanin levels in cotyledons at the seedlingstage, young leaves, and in the stems of secondary shoots.

Potential Applications

G546 appears to affect ABA sensitivity, therefore, G546 or itsequivalogs may have a utility in modifying ABA responses such as seeddormancy and drought tolerance.

In addition, G546 or its equivalogs could be used to alter anthocyaninproduction. The potential utilities of this gene include alterations inpigment production for horticultural purposes, and increasing stressresistance, possibly in combination with another transcription factor.

G561 (SEQ ID NO: 107)

Published Information

G561 is the Arabidopsis gene GBF2 (Schindler et al (1992) EMBO J.11:1261-1273), which was cloned by hybridization to GBF1. GBF2 isconstitutive in both light and dark grown leaves, expressed in roots,and the nuclear import of GBF1 may be light regulated (Terzaghi et al(1997) Plant J. 11:967-982).

Closely Related Genes from Other Species

Close relatives of G561 include a G-box binding protein from Sinapisalba (Y16953; unpublished) and a G-Box binding protein from Raphanussativus (X92102, unpublished).

Experimental Observations

The function of G561 was analyzed using transgenic plants in which thisgene was expressed under the control of the 35S promoter. Plantsover-expressing G561 showed more root growth on potassium free media.Expression of G561 also appears to be constitutive, and may bepreferentially expressed in siliques and moderately inducible with heatstress.

An important aspect of the potassium root growth assay is that plantswere firstly germinated on media with potassium and then transferredonto potassium-free media. G561 overexpressors may have be able tosomehow cope with less potassium, and it is also possible that G561overexpressors accumulated more potassium before they were transferred,which allowed the roots to grow more vigorously after transfer.

As measured by NIR, G561 overexpressors were found to have increasedseed oil content compared to wild-type plants.

Potential Applications

G561 or its equivalogs could be used to increase seedling vigor or plantgrowth in soils that are low in potassium. Potassium is a macronutrientrequired for a variety of basic plant functions which is commonly addedto soil as a fertilizer. The ability to grow plants on low potassiumsoils may save the ecological and material cost of soil fertilization.

G561 or its equivalogs may also be used to manipulate sterolcomposition, and may be used to modify seed oil content in plants, whichmay be very important for the nutritional value and production ofvarious food products.

G562 (SEQ ID NO: 109)

Published Information

G562 is the published Arabidopsis transcription factor GBF3, which wascloned through its hybridization with GBF1 (Schindler et al. (1992) EMBOJ. 11:1261-1273). GBF3, like GBF1 and GBF2, can bind G-box elements as ahomodimer, or as a heterodimer with other bZIP family members. GBF3appears to be highly expressed in roots in comparison to leaves, andrepressed by light. GBF3 binds to G-box elements in the Arabidopsis ADHpromoter in vitro, is induced by ABA in suspension cultures, and isproposed to be the transcription factor responsible for the ABAregulated ADH gene expression (Lu et al. (1996) Plant Cell. 8:847-857).

Closely Related Genes from Other Species

Similar genes to G562 include the B. napus proteins BnGBF1 and BnGBF2(U27107 and U27108) which are strikingly similar to G562 for theirentire lengths. An unpublished Catharanthus roseus G-box binding protein1 protein (AF084971) also has significant homology to G562 outside ofthe conserved domain.

Experimental Observations

G562 appeared to be preferentially expressed in root and flower tissuesby RT-PCR analysis, and expressed at lower levels in other tissues ofthe plant. G562 was induced by heat, drought and osmotic stress inseedlings. The function of G562 was analyzed using transgenic plants inwhich G562 was expressed under the control of the 35S promoter. Plantsoverexpressing G562 were consistently and significantly later flowering,with more crinkled leaves than wild-type plants.

Potential Applications

G562 or its equivalogs could be used to manipulate flowering time inplants.

G567 (SEQ ID NO: 111)

Published Information

G567 was discovered as a bZIP gene in BAC T10P11, accession numberAC002330, released by the Arabidopsis genome initiative.

Closely Related Genes from Other Species

G567 is similar to two bZIP factors from Petroselinum crispum (1806261)and Glycine max (1905785) Similarity between these two proteins and theprotein encoded by G567 extends beyond the conserved domains and thusthey may have a function and utility to G567.

Experimental Observations

The annotation of G567 in BAC AC002330 was experimentally confirmed andthe function of G567 was analyzed using transgenic plants in which G567was expressed under the control of the 35S promoter.

Seedlings overexpressing G567 had slowly opening cotyledons and veryshort roots when grown on MS plates containing glucose. G567 is thuslikely to be involved in sugar sensing or metabolism during germination.

As measured by NIR analysis, plants overexpressing G567 had an increasein total combined seed oil and seed protein content.

G567 appears to be constitutively expressed, and induced in leaves in avariety of conditions.

Potential Applications

G567 or its equivalogs may be useful in manipulating seed oil andprotein content.

G567 or its equivalogs may be used to modify sugar sensing.

In addition to their important role as an energy source and structuralcomponent of the plant cell, sugars are central regulatory moleculesthat control several aspects of plant physiology, metabolism anddevelopment. It is thought that this control is achieved by regulatinggene expression and, in higher plants, sugars have been shown to repressor activate plant genes involved in many essential processes such asphotosynthesis, glyoxylate metabolism, respiration, starch and sucrosesynthesis and degradation, pathogen response, wounding response, cellcycle regulation, pigmentation, flowering and senescence.

Because sugars are important signaling molecules, the ability to controleither the concentration of a signaling sugar or how the plant perceivesor responds to a signaling sugar could be used to control plantdevelopment, physiology or metabolism. For example, the flux of sucrose(a disaccharide sugar used for systemically transporting carbon andenergy in most plants) has been shown to affect gene expression andalter storage compound accumulation in seeds. Manipulation of thesucrose signaling pathway in seeds may therefore cause seeds to havemore protein, oil or carbohydrate, depending on the type ofmanipulation. Similarly, in tubers, sucrose is converted to starch whichis used as an energy store. It is thought that sugar signaling pathwaysmay partially determine the levels of starch synthesized in the tubers.The manipulation of sugar signaling in tubers could lead to tubers witha higher starch content.

Thus, manipulating the sugar signal transduction pathway may lead toaltered gene expression to produce plants with desirable traits. Inparticular, manipulation of sugar signal transduction pathways could beused to alter source-sink relationships in seeds, tubers, roots andother storage organs leading to increase in yield.

G568 (SEQ ID NO: 113)

Published Information

G568 was identified in the sequence of BAC T19K4, GenBank accessionnumber AL022373, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

The PTBF1 gene from Populus×generosa appears to be a potential homologof G568 (GenBank accession no AF288616). PTBF1 expression is associatedwith terminal bud formation.

Experimental Observations

The annotation of G568 in BAC AL022373 was experimentally confirmed.G568 appeared to be preferentially expressed in shoots, roots andflowers and its expression was strongly repressed by the fungalpathogens Erysiphe orontii and Fusarium oxysporum, indicating that insome way it could be a repressor of a defense response.

The function of this gene was analyzed using transgenic plants in whichG568 was expressed under the control of the 35S promoter. Plantsoverexpressing G568 displayed a variety of morphological phenotypes.These morphological phenotypes include narrow leaves, a darker greencoloration, and bushy, spindly, poorly fertile shoots, dwarfing andflowering time alteration. No disease-related phenotype was observed.

Potential Applications

G568 or its equivalogs may be used to manipulate plant architecture andflowering time. The expression pattern of G568 also indicated a use forthis gene or its equivalogs in manipulating the defense response. Thepromoter of G568 may also have some utility as a promoter that can beused to engineer down-regulation of gene expression in response topathogen attack.

G584 (SEQ ID NO: 115)

Published Information

G584 was identified in chromosome IV BAC T6K21 sequence (gene T6K21.10)by the EU Arabidopsis sequencing project as “bHLH protein-like”.

Closely Related Genes from Other Species

A related gene to G584 is Phaseolus vulgaris phaseolin G-box bindingprotein PG1 (U18348). Similarity between G584 and PG1 extends beyond thesignature motif of the family No functional information is available forgene PG1 other than that the protein binds to a G-box motif CACGTG ofthe bean seed storage protein beta-phaseolin gene.

Experimental Observations

The function of G584 was analyzed using transgenic plants in which G584was expressed under the control of the 35S promoter. G584 transgenicplants seemed to produce seed of a larger size than control plants.Analysis of G584 overexpressors revealed no apparent physiological orbiochemical changes when compared to wild-type control plants. Analysisof the endogenous expression level of G584, as determined by RT-PCR,revealed a moderate and constitutive expression level in all Arabidopsistissues examined. G584 transcript level remained similar to wild-typecontrols in all the treatments examined.

Potential Applications

G584 or its equivalogs could be used to produce larger seed size and/oraltered seed morphology, which may positively influence seed storagecharacteristics, appearance and yield.

G585 (SEQ ID NO: 117)

Published Information

G585 has been identified as GL3. It has been shown that G585 regulatestrichome development in Arabidopsis through interaction with GL1 andTTG1 (Payne et al. (2000) Genetics. 156:1349-1362). An increase in thetrichome density was observed in GL3 overexpressed transgenic plants inWS background.

Closely Related Genes from Other Species

G585 protein shares a significant homology to GHDEL65 [Gossypiumhirsutum] protein (PID:g13346182) as well as DEL [Antirrhinum majus]protein (PID:g166428).

Experimental Observations

The sequence of G585 was experimentally determined and the function ofG585 was analyzed using transgenic plants in which G585 was expressedunder the control of the 35S promoter.

Overexpression of G585 reduced trichome density on leaves and stems.Since this phenotype was confined to a proportion of plants in a singleT2 line, it could have been due to co-suppression. To examine this, asecond selection of T1 plants was screened: one out of 18 of theseplants exhibited a clear reduction in trichome density. Since theglabrous effects were eventually seen in two independent lines, theymost likely represented a low penetrance G585 overexpression phenotypeor co-suppression. An increase in the trichome density was not observedin G585 overexpressed transgenic plants in an ecotype background. Theseresults are different from the published information, and may simply bedue to the difference in ecotype used.

As determined by RT-PCR, G585 was uniformly expressed at low level inall tissues tested. Expression level of G585 appears to be enhanced byauxin treatments and repressed by pathogen Fusarium infections.

Potential Applications

G585 or its equivalogs can be used to affect trichome number and/ordistribution. A transcription factor that alters trichome number couldbe used to increase the production of chemical compounds (like essentialoils) that are synthesized and/or stored in trichomes, as well as toprotect plants against damage from a variety of herbivores.

G590 (SEQ ID NO: 119)

Published Information

The sequence of G590 was obtained from the Arabidopsis genome sequencingproject, GenBank accession number Z99707, based on its sequencesimilarity within the conserved domain to other bHLH/Myc relatedproteins. A knockout mutant in G590, named as SPATULA, has also beenisolated and characterized (Heisler et al. (2000) Development128:1089-1098).

Experimental Observations

The function of this gene was studied by knockout analysis and by usingtransgenic plants in which G590 was expressed under the control of the35S promoter.

G590 knockout plants produced more seed oil than wild-type controls.

Overexpression of G590 resulted in a reduction in flowering time and ashorter generation time. Under continuous light conditions, G590overexpressing plants typically produced visible flower budsapproximately one week earlier than wild-type controls. At the time ofbolting, these plants had 4-8 rosette leaves compared with 8-11 in wildtype. Additionally, G590 overexpressor had rather pointed leaves atearly stages of development. The plants also appeared slightly small,yellow, and later, had elongated leaf petioles. No other physiologicaland biochemical alterations were observed in the overexpressiontransgenic plants when compared to wild-type controls.

Gene expression profiling using RT-PCR shows that G590 was relativelyexpressed at higher levels in flowers, siliques and roots. Itsexpression level was unaffected by any of the conditions tested.

Potential Applications

G590 or its equivalogs could be used to increase seed oil content, whichwould be of nutritional value for food for human consumption as well asanimal feeds.

Based on the current analysis of G590 overexpressing plants, G590 or itsequivalogs could be used to manipulate flowering time. A wide variety ofapplications exist for systems that shorten the time to flowering.

G594 (SEQ ID NO: 121)

Experimental Observations

The function of this gene was studied using transgenic plants in whichG594 was expressed under the control of the 35S promoter.

Plants overexpressing G594 showed more disease symptoms followinginfection with the necrotrophic fungal pathogen Sclerotinia sclerotiorumcompared to control plants. In a repeat experiment on individual lines,two lines showed the enhanced susceptibility phenotype. No otherconsistent morphological or biochemical differences were observedbetween G594 overexpressors and wild-type plants.

RT-PCR analysis of G594 transcripts indicate that G594 wasconstitutively expressed in all tissues with exception of roots. Theexpression level of G594 was induced by auxin treatments and repressedby cold, Erysiphe and Fusarium treatments.

Potential Applications

Since G594 transgenic plants have an altered response to thenecrotrophic fungal pathogen Sclerotinia sclerotiorum, G594 or itsequivalogs could be used to manipulate the defense response in order togenerate pathogen-resistant plants.

G597 (SEQ ID NO: 123)

Published Information

G597 was identified in the sequence of BAC F4P9, GenBank accessionnumber AC002332, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G597 has significant homology to a DNA-binding protein PD1 [Pisumsativum] and an Oryza sativa putative AT-Hook DNA-binding protein(PID:12643044).

Experimental Observations

The function of this gene was studied using transgenic plants in whichG597 was expressed under the control of the 35S promoter.

Approximately half of the G597 primary transformants were observed tohave narrow curled rosette leaves. Four T1 plants were also observed tobe later bolting than wild type. However, these phenotypes were notapparent in the initial plantings or re-plants of the T2 populations.

Overexpression of G597 in one line caused an alteration in the leaf cellwall polysaccharide composition. An increase in the percentage of xyloseand a decrease in the percentage of rhamnose was detected. Otherwise,G597 overexpressors behaved similarly to wild-type controls in allbiochemical assays performed.

Based on the RT-PCR analysis, G597 was constitutively expressed in alltissues. Lower expression levels were observed in siliques and caulineleaves. Its expression level was unaffected by any of the conditionstested.

As measured by NIR, G597 overexpressors were found to have increasedseed oil and decreased seed protein content as compared to wild-typeplants.

Potential Applications

G597 or its equivalogs may be used to alter seed protein content inplants, which may be very important for the nutritional value andproduction of various food products.

G598 (SEQ ID NO: 125)

Published Information

G598 was identified in chromosome II BAC T6D20 sequence (gene T6D20.23)by The Institute for Genomic Research as an “unknown protein”.

Experimental Observations

cDNAs representing two splice variants of G598 were identified. Thesesplice variants differ in the 3′ end region and would produce proteinswith different C-termini. The function of G598 was analyzed usingtransgenic plants in which splice variant number 1 of G598 was expressedunder the control of the 35S promoter. G598 overexpressors had higherseed oil content in all three lines tested when measured by NIR. Thesethree lines also showed increased galactose levels when insoluble sugarcomposition was determined. Otherwise, G598 overexpressors behavedsimilarly to wild-type controls in all biochemical assays performed. Thecharacterization of G598 overexpressors revealed no apparentmorphological or physiological changes when compared to wild-typecontrol plants. Analysis of the endogenous expression level of G598, asdetermined by RT-PCR, revealed a moderate and constitutive expressionlevel in all tissues and conditions examined.

One transgenic line showed a reproducible increase in galactose inleaves.

Potential Applications

On the basis of the biochemical analyses performed to date, G598 or itsequivalogs may play a role in the accumulation or regulation of leafinsoluble sugars. Insoluble sugars are among the building blocks ofplant cell walls. Transcription factors that alter plant cell wallcomposition such as galactose have several potential applicationsincluding altering food digestibility, plant tensile strength, woodquality, pathogen resistance and in pulp production. In particular,increasing the insoluble carbohydrate content in various fruits,vegetables, and other edible consumer products will result in enhancedfiber content. Increased fiber content would not only provide healthbenefits in food products, but might also increase digestibility offorage crops.

G598 or its equivalogs could be used to increase seed oil content, whichwould be of nutritional value for food for human consumption as well asanimal feeds.

G634 (SEQ ID NO: 127)

Published Information

G634 was initially identified as public partial cDNAs sequences for GTL1and GTL2 which are splice variants of the same gene (Smalle et al (1998)Proc. Natl. Acad. Sci. USA 95:3318-3322). The published expressionpattern of GTL1 shows that G634 is highly expressed in siliques and notexpressed in leaves, stems, flowers or roots.

Closely Related Genes from Other Species

A close non-Arabidopsis relative of G634 is O. sativa the gt-2 gene (2)which is proposed to bind and regulate the phyA promoter. In addition,the pea DNA-binding protein DF1 (13786451) shows strong homology toG634. The homology of these proteins to G634 extends to outside of theconserved domains and thus these genes are likely to be orthologs ofG634.

Experimental Observations

The boundaries of G634 were experimentally determined and the functionof G634 was investigated by constitutively expressing G634 using theCaMV 35S promoter.

Three constructs were made for G634: P324, P1374 and P1717. P324 wasfound to encode a truncated protein. P1374 and P1717 represent fulllength splice variants of G634; P1374, the shorter of the two splicevariants was used for the experiments described here and the codingsequence of the P1374 clone is provided as the cDNA sequence for G634 inthe Sequence Listing. The longest available cDNA (P1717), confirmed byRACE, had the same ATG and stop codons as the genomic sequence. Onlydata for P1374 are presented here.

Plants overexpressing G634 from construct P1374 had a dramatic increasethe density of trichomes, which were also larger in size. The increasein trichome density was most noticeable on later arising rosette leaves,cauline leaves, inflorescence stems and sepals with the stem trichomesbeing more highly branched than controls. Approximately half of theprimary transformants and two of three T2 lines showed the phenotype.Apart from slight smallness, there did not appear to be any other clearphenotype associated with the overexpression of G634. However, areduction in germination was observed in T2 seeds grown in culture.

RT PCR data showed that G634 was preferentially expressed in flowers andgerminating seedlings, and induced by auxin.

Potential Applications

G634 or its equivalogs may be used to alter trichome structure, functionor density. Trichome glands on the surface of many higher plants produceand secrete exudates that give protection from the elements and pestssuch as insects, microbes and herbivores. These exudates may physicallyimmobilize insects and spores, may be insecticidal or ant-microbial orthey may allergens or irritants to protect against herbivores. Trichomeshave also been suggested to decrease transpiration by decreasing leafsurface air flow, and by exuding chemicals that protect the leaf fromthe sun.

Depending on the plant species, varying amounts of diverse secondarybiochemicals (often lipophilic terpenes) are produced and exuded orvolatilized by trichomes. These exotic secondary biochemicals, which arerelatively easy to extract because they are on the surface of the leaf,have been widely used in such products as flavors and aromas, drugs,pesticides and cosmetics. One class of secondary metabolites, thediterpenes, can effect several biological systems such as tumorprogression, prostaglandin synthesis and tissue inflammation. Inaddition, diterpenes can act as insect pheromones, termite allomones,and can exhibit neurotoxic, cytotoxic and antimitotic activities. As aresult of this functional diversity, diterpenes have been the target ofresearch several pharmaceutical ventures. In most cases where themetabolic pathways are impossible to engineer, increasing trichomedensity or size on leaves may be the only way to increase plantproductivity.

Thus, the use of G634 and its homologs to increase trichome density,size or type may therefore have profound utilities in so calledmolecular farming practices (i.e. the use of trichomes as amanufacturing system for complex secondary metabolites), and inproducing resistant insect and herbivore resistant plants.

G635 (SEQ ID NO: 129)

Published Information

G635 was first identified in the sequence of BAC-end B67864, released bythe Arabidopsis Genome Initiative. Subsequently, the full sequence ofG635 was identified in BAC AB007649, also released by the ArabidopsisGenome Initiative.

Experimental Observations

The boundaries of G635 were experimentally determined and the functionof G635 was analyzed using transgenic plants in which this gene wasexpressed under the control of the 35S promoter. Several plantsover-expressing G635 were non-clonally sectored for chloroplastdevelopment and/or chlorosis. This phenotype seemed to correlateinversely with the expression level of the transgene, and plantsover-expressing the highest amounts of G635 were wild-type inappearance. G635 over-expressing plants were otherwise wild-typebiochemically and physiologically. G635 was constitutively expressed.

In the T2 generation, the bleaching phenotype did not show until plantsstarted to flower, and the bleaching seemed to spread throughout theplant into areas that were previously green. This observation, incombination with the fact that the phenotype seems to be correlated withlow expression of the gene, indicated that the phenotype was induced bysilencing of G635.

A number of plants transformed with G635 had a variegated appearance.

Potential Applications

Based on the phenotype produced when G635 or its equivalogs may have autility as a regulator of chloroplast development. In addition, G635 maybe a herbicide target—if its activity or expression could be reducedusing a small molecule, it could potentially kill the plant by causingchlorosis. G635 could also be developed into a marker for silencing inArabidopsis.

The variegated phenotype associated with G635 or equivalogoverexpression may find utility in ornamental applications.

G636 (SEQ ID NO: 131)

Published Information

G636 was identified through partial EST AA395524, released by MichiganState University. The entire sequence of G636 was later identified inBAC F7O12, accession number F7O12, released by the Arabidopsis genomeinitiative.

Closely Related Genes from Other Species

G636 is closely related to the Pisum sativum DNA-binding protein DF1,accession number AB052729, which may bind to light regulatory elements.

Experimental Observations

The 5′ boundary of G636 was determined and the function of G636 wasanalyzed by constitutively expressing the gene using the CaMV 35Spromoter. Overexpression of G636 resulted in premature senescence ofleaves and reduced plant size and fertility. No other phenotypicalterations were noted as a result of physiological or biochemicalanalyses.

G636 was constitutively expressed.

Potential Applications

G636 or its equivalogs may be used to alter senescence responses inplants. Although leaf senescence is thought to be an evolutionaryadaptation to recycle nutrients, the ability to control senescence in anagricultural setting has significant value. For example, a delay in leafsenescence in some maize hybrids is associated with a significantincrease in yields and a delay of a few days in the senescence ofsoybean plants can have a large impact on yield. Delayed flowersenescence may also generate plants that retain their blossoms longerand this may be of potential interest to the ornamental horticultureindustry.

G638 (SEQ ID NO: 133)

Published Information

G638 was identified in the sequence of BAC F17C15, GenBank accessionnumber AL162506, released by the Arabidopsis Genome Initiative. Duringthe course of its functional analysis, G638 was identified as the PETALLOSS gene (Griffith et al. (1999) Development: 126:5635-5644). The PETALLOSS knockout mutant displays a variety of flower phenotypes, moststrikingly characterized by a reduction in the number of petals. Inaddition to flower organ number, organ identity, shape and orientation,particularly of petals, is altered.

Closely Related Genes from Other Species

A relative of G638 is a Medicago truncatula gene represented by the ESTBF646615, which was isolated from an elicited cell culture cDNA library.

Experimental Observations

The boundaries of G638 were experimentally determined and the functionof G638 was analyzed using transgenic plants in which this gene wasexpressed under the control of the 35S promoter. Expression of G638causes severe alterations of plant development. The most strikingfeature of these overexpressor plants was that they have multipetallateflowers. In early flowers, some homeotic conversion had occurred betweensome organs of the flower. In all flowers made after these earlyflowers, petal number had been altered. Up to eight petals wereconsistently observed on plants that flowered, and as the plants grewolder, the number of petals on new flowers was reduced from eight toabout five. This phenotype was somewhat opposite to the phenotypeobserved with PETAL LOSS knockout plants and confirms a role for G638 incounting or maintaining petal number within the Arabidopsis flower. Inaddition to the flower phenotype, G638 caused alterations in phyllotaxy,leaf shape and caused plants to be sterile. G638 appears to beconstitutively expressed.

Potential Applications

G638 or its equivalogs could be used to manipulate plant architectureand leaf shape, in particular this gene could be used to increase ordecrease petal number in flowers. Overexpression of G638 also causessterility, indicating there may be some use for this gene in engineeringsterility into commercially relevant species.

G652 (SEQ ID NO: 135)

Published Information

G652 was identified in the sequence of BAC clones F26H11 and F7024,GenBank accession number AC006264, released by the Arabidopsis GenomeInitiative.

Experimental Observations (Knockout)

G652 appears to be constitutively expressed at medium levels in alltissues and environmental conditions tested as determined by RT-PCRanalysis. Expression of G652 was not detected in other tissues. A linehomozygous for a T-DNA insertion in G652 was used to determine thefunction of this gene. The T-DNA insertion of G652 was approximately 75%into the coding sequence of the gene and therefore was likely to resultin a null mutation. Plants homozygous for a T-DNA insertions within G652displayed a spectrum of developmental abnormalities, particularly at theearly seedling stage. These phenotypes were variable within thepopulation, suggesting that other factors might be influencing thepenetrance of the phenotype. For example, seedlings were small andfilled with anthocyanins. Almost all the seedlings had defects incotyledons ranging from unusual shape to fusions. Many seedlings did notsurvive, and those that did grew slowly. Fertility was reduced comparedto controls, senescence delayed, and siliques were often rather short.The reason for this poor fertility was unclear. Many flowers had areduced number of stamens (4-5 of these organs rather than 6).Interestingly, the absent stamen(s) were usually one or both of theshorter pair. Seeds produced by knockouts of G652 plants were somewhatwrinkled and misshapen.

The G652 knockout line had a reproducible increase in the leafglucosinolate M39480. It also showed a reproducible increase in seedalpha-tocopherol. A decrease in seed oil as measured by NIR was alsoobserved.

Experimental Observations (Overexpressor)

The function of G652 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G652 resulted in plants that were small and slow developing. Manyplants died at an early stage of growth. The two lines that weremorphologically examined in the T2 generation were small and showedpremature senescence of rosette leaves.

35S::G652 plants were wild-type in physiological analyses that wereperformed.

Potential Applications

G652 or its equivalogs could be used to manipulate seed tocopherolcomposition and seed structure and to alter glucosinolate composition inleaves.

G652 may also be useful for modifying glucosinolate content. Increasesor decreases in specific glucosinolates or total glucosinolate contentmight be desirable depending upon the particular application.

G663 (SEQ ID NO: 137)

Published Information

G663 was identified from the Arabidopsis EST sequence, H76020, based onits sequence similarity within the conserved domain to other Myb familymembers in Arabidopsis. This gene was named MYB90 (Kranz et al. (1998)Plant J. 16:263-276). Reverse Northern data suggested G663 is expressedhighly in leaves, siliques, and flowers and is induced by ethylenetreatment.

Experimental Observations

The function of G663 was analyzed by its ectopic overexpression inplants. G663 overexpressors had constitutive anthocyanin production inseeds and roots. One line had higher anthocyanin production in leaftissue as well. In other overexpressing lines, constitutive anthocyaninproduction was noted in trichomes and leaf margins. The overproductionof pigment in select tissues suggests there may be another transcriptionfactor with which G663 interacts to activate the pathway. Using the cornsystem as a model, the interacting protein may be a bZIP liketranscription factor.

RT-PCR analysis of the endogenous levels of G663 indicated that thisgene was expressed primarily in siliques and seedlings. Array dataconfirmed the high levels in silique and also detected high levels ofG663 in germinating seed tissue. G663 transcripts were also inducedabove basal levels by all stress treatments tested except by infectionwith Erysiphe orontii. These data were consistent with G663 beinginvolved in the anthocyanin biosynthetic pathway, which is part of acommon multi-stress response pathway.

Potential Applications

The potential utilities of this gene or its equivalogs includesalterations in pigment production for horticultural purposes, andpossibly increasing stress resistance in combination with anothertranscription factor. Flavonoids have antimicrobial activity and couldbe used to engineer pathogen resistance. Several flavonoid compoundshave health promoting effects such as the inhibition of tumor growth andcancer, prevention of bone loss and the prevention of the oxidation oflipids. Increasing levels of condensed tannins, whose biosyntheticpathway is shared with anthocyanin biosynthesis, in forage legumes is animportant agronomic trait because they prevent pasture bloat bycollapsing protein foams within the rumen. For a review on the utilitiesof flavonoids and their derivatives, refer to Dixon et al. ((1999)Trends Plant Sci. 10: 394-400).

G664 (SEQ ID NO: 139)

Published Information

G664 was identified from the Arabidopsis EST sequence, N38154, based onits sequence similarity within the conserved domain to other Myb familymembers in Arabidopsis. The Myb consortium named this gene MYB4 (Kranzet al. (1998) Plant J. 16: 263-276). Reverse Northern data suggestedG664 is expressed highly in silique tissue with a low level ofexpression detected in all other tissues.

Closely Related Genes from Other Species

G664 shows extensive homology to the tomato gene THM27 (X95296) and thebarley gene (X70877).

Experimental Observations

The function of G664 was analyzed through its ectopic overexpression inplants. G664 overexpressors germinated better and then developed morerapidly in cold conditions (8° C.) than wild-type controls. Nodifferences in germination rates were observed on control MS media or inresponse to any other stress. Array data indicated that G664 wasnormally expressed primarily in root, shoot and silique.

Potential Applications

The potential utility of this gene or its equivalogs is to conferimproved cold germination and/or growth. The germination of many cropslike cotton is very sensitive to cold temperatures, a gene that wouldallow germination and seedling vigor in the cold would have tremendousutility in allowing seeds to be planted earlier in the season with ahigh rate of survivability.

G674 (SEQ ID NO: 141)

Published Information

G674 is a member of the (R1)R2R3 subfamily of myb transcription factors.G674 was identified in the sequence of BAC clone T2J13 with accessionnumber AL132967 released by the Arabidopsis genome initiative. G674 hasalso been referred to as MYB45 (Kranz H D, et al. (1998) Plant J.16:263-276). No information is available about the function(s) of G674.

Experimental Observations

The function of G674 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G674transformants were generally rather smaller than wild-type controls, andpossessed rounded, dark green leaves that were sometimes pointed upward.Overexpression of G674 also resulted in an increase in seedglucosinolate M39501 in two T2 lines. No other phenotypes wereassociated with the overexpression of G674.

RT-PCR analysis of endogenous levels of G674 indicated that this genewas expressed in all tissues except shoot. Expression levels of G674seemed to vary in response to stress-related treatments.

Potential Applications

On the basis of the analyses performed to date, G674 or its equivalogscould be used to alter plant growth and development. In addition,overexpression of G674 caused changes in the seed glucosinolate profile.

G676 (SEQ ID NO: 143)

Published Information

G676 was identified from an Arabidopsis EST, N96391, based on itssequence similarity to other members of the Myb family within theconserved domain. The Myb consortium named this gene MYB66 (Kranz H D,et al. (1998) Plant J. 16:263-276) and in a report by Lee et al ((1999)Cell 1999 24; 99:473-483) a detailed functional analysis of G676, or“werewolf”, is described. Werewolf (WER) is involved inposition-dependent patterning of epidermal cell types. Transcripts werelocalized to root epidermal cells that will develop into non-hair cells.WER was shown to regulate the position-dependent expression of GLABRA2,to interact with the maize R gene, and to act as an antagonist to themyb protein CAPRICE (G225). These authors do not report altered trichomepositioning in their 35S:wer overexpressors.

Experimental Observations

The function of G676 was analyzed through its ectopic overexpression inplants. Morphologically, the plants are small, and partially glabrous onthe upper surface of the leaf. Ectopic trichomes developed on theunderside of the leaf in one line. Lee et al (1999) Cell 99: 473-483)fail to report altered trichome phenotypes in the leaves of the 35S:wereoverexpression lines. The present lines showed a higher degree ofoverexpression, which could explain the small stature of the plants aswell.

RT-PCR analysis of the endogenous levels of G676 indicated that thisgene was expressed primarily in roots with a low level of expression insiliques and seedlings. G676 transcripts were not induced significantlyabove basal levels by any stress-related treatments tested. Indisease-related treatments where whole seedlings were harvested,transcripts were detectable but not above basal levels. This may berelated to the gene's root expression. G676 transcripts were not foundin Fusarium oxysporum treated seedlings; it is possible this treatmentrepresses G676 expression in the roots.

Potential Applications

The potential utility of G676 or its equivalogs is the production ofectopic trichomes on the surface of the leaf. It would be of significantagronomic value to have plants that exhibit greater numbers of glandulartrichomes producing essential oils for the pharmaceutical and foodindustries, as well as oils that protect plants against insect andpathogen attack.

G680 (SEQ ID NO: 145)

Published Information

G680 or LHY (late elongated hypocotyl) is an unusual Myb transcriptionfactor in that it contains a single Myb repeat instead of the two repeatsequences found in the majority of plant Myb genes (R2R3Mybs). There areover 30 members of this single repeat Myb-related subfamily in theArabidopsis genome. Both signature repeats in R2R3Myb domain arerequired for sequence specific DNA binding. However, the Myb-relatedsubfamily with a single repeat domain are also able to bind to DNA in asequence-specific manner (Baranowskij et al. (1994) EMBO J. 13:5383-5392; Feldbrugge et al. (1997) Plant J. 11: 1079-1093) and aretherefore thought to function as transcription factors.

G680 or LHY overexpression affects many processes associated with thecircadian clock including, the rythmicity in both leaf movement, and theexpression of CAB and CCR2 genes, as well as photoperiodic control offlowering time (Schaffer et al. (1998) Cell 93: 1219-1229). Otherreported pleiotropic effects include elongated hypocotyls, elongatedpetioles, and pale leaves (Schaffer et al. (1998) Cell 93: 1219-1229).All of these phenotypes could potentially be explained by the impairmentof circadian clock function. LHY shows a high degree of homology toCCA1, another protein implicated in circadian clock function (Wang etal. (1997) Plant Cell 9: 491-507).

Experimental Observations

The function of G680 was analyzed through its ectopic overexpression inplants. G680 overexpressors were late flowering under both short andlong day conditions, however, the late flowering phenotype appeared moreconsistently under short day conditions. The overexpressors were darkergreen in color compared to the wild-type controls at later stages ofdevelopment. This was inconsistent with the published phenotype, whichindicates the plants have less chlorophyll, and are pale in color(Schaffer et al. (1998) Cell 93: 1219-1229). Preliminary data indicatedthat a vernalization treatment applied to germinating seedlingspartially overcame the delay in flowering in the G680 overexpressors.Vernalized plants showed an approximate 35% reduction in leaf number onaverage compared to non-vernalized controls. Overexpression of G680 inplants also resulted in sensitivity to media containing high glucose ina germination assay, indicating a potential role for G680 in sugarsensing.

As determined by RT-PCR, G680 was uniformly expressed in all tissuestested. RT-PCR data also indicated a moderate induction of G680transcripts accumulation upon drought treatment, and Erysiphe treatmentcould repress the expression of this gene.

Potential Applications

G680 or its equivalogs may be used to alter sugar sensing in plants.Sugars are key regulatory molecules that affect diverse processes inhigher plants including germination, growth, flowering, senescence,sugar metabolism and photosynthesis. Sucrose is the major transport formof photosynthate and its flux through cells has been shown to affectgene expression and alter storage compound accumulation in seeds(source-sink relationships). Glucose-specific hexose-sensing has beendescribed in plants and implicated in cell division and repression of‘famine’ genes (photosynthetic or glyoxylate cycles). The potentialutilities of a gene involved in glucose-specific sugar sensing are toalter energy balance, photosynthetic rate, carbohydrate accumulation,biomass production, source-sink relationships, and senescence.

Potential utilities of G680 or its equivalogs also include theregulation of flowering time. An area in which late flowering might beuseful include crops where the vegetative portion of the plant is themarketable portion. In this case, it would be advantageous to prevent ordelay flowering in order to increase yield. Prevention of floweringwould also be useful in these same crops in order to prevent the spreadof transgenic pollen and/or to prevent seed set.

A vernalization treatment applied to germinating G680 seedlings willpartially overcome the delay in flowering in the G680 overexpressors.Vernalized plants showed an approximate 35% reduction in leaf number onaverage compared to non-vernalized controls. Various late floweringmutants are partially rescued by GA applications (Chandler et al. (1994)J. Exp. Bot. 45: 1279 1288). Thus it is possible that G680 could be usedto increase the vegetative phases of development in order to increaseyield and then triggered to flower via a cold treatment or a gibberellicacid application.

G682 (SEQ ID NO: 147)

Published Information

G682 was identified from the Arabidopsis BAC, AF007269, based onsequence similarity to other members of the Myb family within theconserved domain.

Experimental Observations

The function of G682 was analyzed through its ectopic overexpression inplants. G682 overexpressors were glabrous, had tufts of more root hairsand germinated better under heat stress conditions. Older plants werenot more tolerant to heat stress compared to wild-type controls.

RT-PCR analysis of the endogenous levels of G682 transcripts indicatedthat this gene was expressed in all tissues tested, however, a very lowlevel of transcript was detected in roots and shoots. Array tissue printdata indicated that G682 was expressed primarily, but not exclusively,in flower tissue.

An array experiment was performed on one G682 overexpressing line. Thedata from this one experiment indicated that this gene could be anegative regulator of chloroplast development and/or light dependentdevelopment because the gene Albino3 and many chloroplast genes arerepressed. Albino3 functions to regulate chloroplast development(Sundberg et al (1997) Plant Cell 9:717-730). The gene G682 was itselfinduced 20-fold. Other than a few additional transcription factors, veryfew genes are induced as a result of the ectopic expression of G682.

A number of plants transformed with G682 lacked trichomes.

Plants overexpressing paralogs of G682, including G225, G226 and G1816,have similar traits as plants that overexpress G682. These traitsinclude reduction or lack of trichomes and increased root hairs, thelatter indicating improved resistance to osmotic stress Plantsoverexpressing G676 and G1332 also have reduced trichome density. G676and G1332 share 52% (21 of 40 residues) and 60% (24 of 40 residues)identity with G682, respectively, and 62% (20 of 32 residues) and 68%(22 of 32 residues) with the conserved domain of G682, respectively.

The polypeptide sequence of G682 shares 70% (50 of 71 residues), 66% (37of 56 residues), and 57% (43 of 75 residues) identity with the conserveddomains of G225, G226 and G1816, respectively. The conserved domain ofG682 shares 86% (32 of 37 residues), 63% (23 of 36 residues), and 69%(25 of 36 residues) identity with the conserved domains of G225, G226and G1816, respectively.

In addition to the paralogous sequences disclosed above, orthologoussequences from other plant species were also identified using BLASTanalysis. Such orthologous sequences, together with the paralogoussequences were determined to be members of the G682 TF family ofMyb-related proteins (equivalogs). The paralogous sequences and theorthologous sequences were aligned using MACVECTOR software (Accelrys,Inc.). The software program also generated an exemplary consensus aminoacid residue sequence of the aligned sequences.

As shown in FIGS. 3A and 3B, the orthologous sequences shared aconsensus sequence with the conserved domain of G682 (amino acidresidues 27-63 of SEQ ID NO:148) and also shared identity with regionsflanking the conserved domain (flanking regions). In particular, G682shared a region of the conserved domain with sequences from soy (Glycinemax; SEQ ID NOs: 1084, 1085, 1086, 1083, 1087, and 1088), rice (Oryzasativa; SEQ ID NOs: 559, 1082, and 1081), and maize (corn) (Zea mays;SEQ ID NOs: 1089 and 1090).

An exemplary consensus of the conserved domain of the G682 TF family ofMyb-related proteins isVal-Xaa-Met/Phe-Ser/Thr-Gln/Glu-Xaa-Glu-Glu-Asp-Leu-Val-Xaa-Arg-Met-His/Tyr-Lys/Arg-Leu-Val-Gly-Asp/Glu-Arg/Lys-Trp-Glu/Asp-Leu/Ile-Ile-Ala-Gly-Arg-Ile/Val-Pro-Gly-Arg,where Xaa is any amino acid residue. An alternative exemplary consensusof the conserved domain isVal-Xaa-Met/Phe-Ser/Thr-Gln/Glu-Xaa-Glu-Glu-Asp-Leu-Val-Ser-Arg-Met-His-Arg-Leu-Val-Gly-Asn-Arg-Trp-Glu-Leu-Ile-Ala-Gly-Arg-Ile-Xaa-Gly-Arg,where Xaa is any amino acid residue. A further alternative exemplaryconsensus of the conserved domain isVal-Xaa-Met/Phe-Ser/Thr-Gln/Glu-Xaa-Glu-Glu-Asp-Leu-Val-Ser-Arg-Met-Tyr-Xaa-Leu-Val-Gly-Asn/Glu-Arg-Trp-Ser-Leu-Ile-Ala-Gly-Arg-Ile-Pro-Gly-Arg,where Xaa is any amino acid residue.

Potential Applications

The potential utility of this gene or its equivalogs is to confer heattolerance to germinating seeds.

G682 or its equivalogs could be used to alter trichome number anddistribution in plants. Trichome glands on the surface of many higherplants produce and secrete exudates, which give protection from theelements and pests such as insects, microbes and herbivores. Theseexudates may physically immobilize insects and spores, may beinsecticidal or ant-microbial or they may allergens or irritants toprotect against herbivores. Trichomes have also been suggested todecrease transpiration by decreasing leaf surface air flow, and byexuding chemicals that protect the leaf from the sun.

G715 (SEQ ID NO: 149)

Published Information

G715 is a member of the Hap5 subfamily of CCAAT-box transcriptionfactors. G715 corresponds to Hap5a, and was found to be expressedubiquitously in Arabidopsis (Edwards, et al. (1998) Plant Physiol. 117:1015-1022).

Experimental Observations

The complete sequence of G715 was determined. The function of this genewas analyzed using transgenic plants in which G715 was expressed underthe control of the 35S promoter. The expression of G715 appeared to beubiquitous.

G715 overexpressors had higher seed oil content in the lines tested byNIR.

Potential Applications

G715 or its equivalogs could be used to increase seed oil content, whichwould be of nutritional value for food for human consumption as well asanimal feeds.

G720 (SEQ ID NO: 151)

Published Information

G720 was described as APRR2, for Arabidopsis pseudo-response regulator(Makin et al. 2000 Plant Cell Physiol. 41:791-803). This designationreflects the fact that the protein contains significant homology to areceiver domain at the N-terminus, but has a glutamate in place of theconserved aspartate residue that is phosphorylated by a histidine kinaseor phosphotransmitter protein.

Closely Related Genes from Other Species

G720 showed significant similarity to a drought-induced M. truncatulaEST, GenBank accession number BG450227, that encodes a pseudo-receiverdomain. The sequence similarity is high enough to suggest that the twoproteins are orthologs, and the fact that G720 was also drought-inducedis consistent with this hypothesis. Other ESTs from tomato and potato(BG642566, BG128919, BG129142, and BG887673) also showed high similarityto G720 and represent potential orthologs.

Experimental Observations

The complete sequence of G720 (SEQ ID NO: 151) was determined A linehomozygous for a T-DNA insertion in G720 and lines overexpressing G720under the 35S promoter were used to determine the function of this gene.The T-DNA insertion in G720 was approximately half-way into the codingsequence, just before the conserved domain, and therefore should resultin a null mutation. G720 knockout mutants were slightly more sensitiveto freezing than the wild-type controls when the seedlings werecold-acclimated prior to freezing. G720 overexpressing lines were moretolerant to freezing. When seedlings were frozen at −10° C. for 20hours, the G720 plants recovered better compared to the wild-typecontrol in two separate experiments. G720 was induced by ABA, salt,osmotic stress, drought, heat, and auxin. The combination of enhancedsensitivity to freezing in the knockout mutants, enhanced resistance inthe overexpressing lines, and the induction pattern of G720 comprisedstrong evidence that G720 functions in regulation of dehydrationtolerance, as freezing is a form of dehydration stress.

Plants overexpressing G720 also showed reduced time to flowering in theT1 generation. One third of the 35S::G720 T1 seedlings, from each of twoseparate batches, flowered markedly earlier (up to 1 week sooner,24-hour light conditions) than controls plants. All of the T1 linesshowed high levels of G720 overexpression (determined by RT-PCR). Threeearly flowering T1 plants were selected for further study. However, noneof these lines flowered early in the T2 generation, suggesting thatactivity of the transgene might have been reduced between thegenerations

Potential Applications

G720 or its equivalogs could be used to increase freezing tolerance inplants, and tolerance to other forms of moisture stress such as drought.

G736 (SEQ ID NO: 153)

Published Information

G736 was discovered as a full length EST clone. It was subsequentlylocalized to BAC AC002341.

Experimental Observations

RT-PCR analysis of the endogenous levels of G736 indicated that thisgene was expressed at low to medium levels in all tissues tested. Inaddition, there was no induction of G736 above its basal level inresponse to environmental stress treatments.

Two out of three G736 overexpressing lines exhibited a severe lateflowering phenotype in both the T1 and T2 generation, the third line waslate flowering in the T1 generation but the phenotype was lost in thesubsequent generation, most likely due to silencing of the transgene.All three lines exhibited elongated petioles in both generations, and intwo of the T1 lines, failure of the siliques to elongate was alsoobserved. This phenotype was lost in the subsequent generation.

Potential Applications

Overexpression of G736 and its equivalog may be used to substantiallydelay flowering. A wide variety of applications exist for genes thateither lengthen or shorten the time to flowering, or for systems ofinducible flowering time control. In particular, in species where thevegetative parts of the plants constitute the crop and the reproductivetissues are discarded, it would be advantageous to delay or preventflowering. Extending vegetative development could bring about largeincreases in yields. Additionally, a major concern is the escape oftransgenic pollen from GMOs to wild species or so-called organic crops.Systems that prevent vegetative transgenic crops from flowering wouldeliminate this worry.

G748 (SEQ ID NO: 155)

Published Information

A cDNA sequence for G748 was deposited in GenBank by Abbaraju and Oliveron Aug. 4, 1998. G748 encodes a protein containing a D of zinc-fingerdomain that was found to bind the H-protein promoter. The H protein is acomponent of the glycine decarboxylase multienzyme complex, thatcomprises over one-third of the soluble proteins in mitochondriaisolated from the leaves of C3 plants (Oliver et al. (1995) Bioenerg.Biomembr. 27: 407-414). A published function for G748 is a putativeregulatory role in H-protein gene expression, suggested by thepromoter-binding data.

Closely Related Genes from Other Species

Close relatives to G748 include a rice gene (GB accession # BAA88190)and a pumpkin gene (GB accession # D45066). In both cases, thesimilarity extends beyond the conserved DNA-binding domain, whichsuggests the genes could be orthologs of G748. The pumpkin gene encodesan ascorbate oxidase promoter-binding protein, suggesting that theproduct of G748 could also bind that promoter.

Experimental Observations

A cDNA sequence was isolated and used to produce transgenic plantsoverexpressing G748. Overexpression of G748 resulted in a late floweringphenotype. Transgenic plants were generally large and dark green withmore rosette leaves. Stems were thicker and more vascular bundles werenoticeable in transverse sections. G748 overexpressors also producedmore lutein in seeds (consistently observed in three lines). The highlutein phenotype was confirmed in a repeat experiment. The physiology ofthe plant was similar to that of the controls. In wild-type plants, G748was constitutively expressed, although at lower levels at the seedlingstage. Expression levels were lower upon infection with E. orontii andFusarium.

Potential Applications

Experimental data showed that G748 or its equivalogs can be used todelay flowering in transgenic plants.

Arabidopsis plants overexpressing G748 produced more lutein in seeds.

Plants transformed with G748 had modified stem morphology and vascularbundles and may be used to affect overall plant architecture.

G779 (SEQ ID NO: 157)

Published Information

G779 has been previously identified; fruits from a ind1 knockout mutantplants do not show cell differentiation in the dehiscence zone(Liljegren et al. (2000) Abstracts 11th Intl. Conf. Arabidopsis Res.,Madison, Wis., pp. 179). These results suggest that G779 may mediatecell differentiation during Arabidopsis fruit development.

Closely Related Genes from Other Species

G779 is closely related to a Brassica rapa subsp. Pekinensis cDNAisolated from flower bud (acc#AT002234).

Experimental Observations

The function of G779 was analyzed using transgenic plants in which G779was expressed under the control of the 35S promoter. Morphologicalanalysis of overexpressors indicated that primary transformants of G779had high levels of anthocyanin in seedlings, produced small plants withdisorganized rosettes and short internodes, and many had flowerabnormalities. The transformants with flower abnormalities showedconversion of sepals to carpels. The most severely affected had fullconversion of sepals to carpels with ovules, stigmatic tissue on petalsand stamens, and in some cases showed organ fusions. In the severe caseof one T1 line, some inflorescences showed no flowers at all. Plantswith a weak phenotype showed only small patches of stigmatic tissue onsepals. The floral phenotypes decreased acropetally. The plants showingthe strongest phenotypes were essentially sterile, and did not produceT2 progeny for further analysis.

The phenotype produced by overexpressing G779 and G1499 was similar inthe aspects of flower structures. Cluster analysis using basichelix-loop-helix motif revealed that both proteins of G779 and G1499 areclosely related. The fact that expression of G779 was induced by auxintreatment in the rosette leaves indicates that G779 may play some kindof role in the auxin signal transduction pathway.

Potential Applications

G779 or its equivalogs could be used to modify plant architecture anddevelopment, including flower structure. If expressed under aflower-specific promoter, it might also be useful for engineering malesterility. Because expression of G779 is flower, embryo and siliquespecific, its promoter could be useful for targeted gene expression inthese organs.

G789 (SEQ ID NO: 159)

Published Information

A partial sequence of G789 was identified from an EST clone (GenBankaccession number T41998).

Experimental Observations

G789 was initially identified as a public EST (GenBank accession numberT41998) and subsequently a full length library clone was identified. Thefunction of G789 was analyzed using transgenic plants in which G789 wasexpressed under the control of the 35S promoter.

Overexpression of G789 reduced the time to flowering under continuouslight conditions; this phenotype was most prevalent in the T2 generationand was noted in all three of the lines analyzed.

Transgenic plants overexpressing G789 were more sensitive to theherbicides glyphosate and acifluorfen and to oxidative stress caused byrose bengal compared to wild-type controls. Furthermore, G789overexpressing lines were more susceptible to infection with Sclerotiniasclerotiorum when tested as mixed lines in two repeat experiments. Thisdisease susceptibility phenotype did not repeat when individual lineswere tested. It is well known that oxidative stress is a component of aplant defense response to pathogen and therefore, the diseasesusceptibility phenotype could thus be related to a general sensitivityto oxidative stress.

Based on the RT-PCR analysis, G789 was constitutively expressed in alltissues; its expression level was unaffected by any of the conditionstested.

Potential Applications

Based on the current analysis of G789 overexpressing plants, G789 or itsequivalogs could be used to manipulate flowering time.

Since G789 activity has been shown to be required for the protection ofArabidopsis plants against oxidative stress, G789 or its equivalogscould be used to manipulate defenses against abiotic and biotic stressessuch as disease, UV-B radiation, ozone pollution and herbicideapplication.

G801 (SEQ ID NO: 161)

Published Information

A partial sequence for G801 was identified from EST clones (GenBankaccession numbers N97289, H36373 and Z32574).

Experimental Observations

G801 is a proprietary sequence initially identified as three partialpublic ESTs (GenBank accession numbers N97289, H36373 and Z32574).Subsequently, a full length library clone was identified. The functionof G801 was analyzed using transgenic plants in which G801 was expressedunder the control of the 35S promoter. Morphological analysis revealedthat a minority of primary transformants of G801 were dark green andlate flowering. However, T2 lines derived from three late-floweringlines showed no flowering time differences from control plants. Plantoverexpressing G801 showed more seedling vigor when germinated on mediacontaining high salt compared to wild-type control plants. All threeoverexpressing lines showed similar degrees of tolerance. In addition,overexpression of G801 in Arabidopsis resulted in an increase in seedoil content. This phenotype was observed in a single line.

Potential Applications

The potential utilities of this gene or its equivalogs include theability to confer salt tolerance during the germination stage of a cropplant. This would most likely impact survivability and yield.Evaporation of water from the soil surface causes upward water movementand salt accumulation in the upper soil layer, where the seeds areplaced. Thus, germination normally takes place at a salt concentrationmuch higher than the mean salt concentration in the whole soil profile.

In addition, G801 or its equivalogs may be used to increase seed oil incrop plants.

G849 (SEQ ID NO: 163)

Published Information

The transcription factor G849 is an Arabidopsis homolog of parsleyBPF-1, a pathogen inducible DNA-binding protein. BPF-1, Box-P BindingFactor 1, was reported by da Costa e Silva et al. ((1993) Plant Journal4:125-135) to bind specifically to the P-box sequence motif of thephenylalanine ammonia lyase promoter, a key enzyme of thephenylpropanoid metabolism. G849 is found in the sequence of chromosome3, BAC T2E22 (GenBank AC069474.4 GI:12321944), released by theArabidopsis Genome Initiative. The start and stop codons were correctlypredicted.

Experimental Observations

NIR analyses performed on G849 knockout plants revealed increased totalcombined seed oil and protein content.

RT-PCR analysis of the endogenous level of G849 transcripts revealedhigh constitutive expression in all tissues examined, with the exceptionof germinated seed. A detectable but low level of G849 transcripts wasobserved in germinated seeds. G849 transcript level increasedsignificantly upon auxin, ABA, cold, heat and salt treatment, as well asseven days post-inoculation with Erysiphe orontii.

Potential Applications

Based on the knockout analyses, G849 or its equivalogs may be used tomodify seed oil and protein content.

The null mutant of G849 had altered seed phytosterol composition, adecease in beta-sitosterol, as well as changes in leaf insoluble sugars.Phytosterols are an important source of precursors for the manufactureof human steroid hormones by semisynthesis. Sitosterols andstigmasterols, not campesterol, are the preferred sources from seedcrops. Phytosterols and their hydrogenated derivatives phytostanols alsohave proven cholesterol-lowering properties.

G859 (SEQ ID NO: 165)

Published Information

G859 corresponds to MXK3.30 (BAB 10332). The high level of sequencesimilarity between G859 and FLOWERING LOCUS C (FLC; Michaels et al.(1999) Plant Cell 11, 949-956; Sheldon et al., (1999) Plant Cell 11,445-458) has been described previously (Ratcliffe et al. (2001) PlantPhysiol. 126:122-132). G859 has also been referred to as AGL31(Alvarez-Buylla et al. (2000) Plant J. 24:457-466).

Experimental Observations

G859 was recognized as a gene highly related to Arabidopsis FLC, and toMADS AFFECTING FLOWERING 1. FLC acts as a repressor of flowering(Michaels (1999) Plant Cell 11, 949-956; Sheldon et al. (1999) PlantCell 11, 445-458). Similarly, G157/MAF1 can cause a delay in floweringtime when overexpressed (Ratcliffe et al. (2001) Plant Physiol.126:122-132).

The function of G859 was studied using transgenic plants in which thisgene was expressed under the control of the 35S promoter. Overexpressionof G859 modified the timing of flowering, with very high levels of G859activity delaying the floral transition in the Columbia ecotype. Noalterations were detected in 35S::G859 plants in the physiological andbiochemical analyses that were performed.

Under continuous light conditions, the majority of 35S::G859 primarytransformants (overexpressing a construct containing a full-length cDNA,P1688) were earlier flowering than wild-type controls. This result wasobserved in multiple independent batches of T1 plants and in eithercontinuous or 12 hour light conditions. However, in each selection ofprimary transformants, a small number of lines were late flowering.RT-PCR analyses demonstrated that all T1 plants overexpressed thetransgene, but that the highest levels of expression were found in thelate flowering transformants. Comparable results were also obtained whenplants were transformed with a construct (P376) containing a shortersplice-variant of G859. The effects on flowering time caused byoverexpression of G859, and the dependence of those effects on thetransgene expression levels, mirror results previously obtained forG157/MAF1 (Ratcliffe et al. (2001) Plant Physiol. 126:122-132).

Seed was taken for T2 analyses from two late flowering primarytransformants, and a T1 plant that had been early flowering. The progenyof the former two lines all appeared markedly late flowering, while theT2 plants from the third line were marginally late flowering. Noconvincing early flowering was observed in any the three T2 populations.Thus, in the second generation, the predominant effect of G859 activitywas delayed flowering. In a follow-up experiment it was found that lateflowering 35S::G859 T2 plants were photoperiod responsive, and were notsensitive to extensive vernalization treatments.

Potential Applications

G859 or its equivalogs could be used to alter flowering time.

G864 (SEQ ID NO: 167)

Published Information

G864 was identified in an Arabidopsis EST (H37693). G864 appears as geneAT4g23750 in the annotated sequence of Arabidopsis chromosome 4(AL161560).

Experimental Observations

G864 was discovered and initially identified as a public ArabidopsisEST.

The complete sequence of G864 was determined, and G864 was found to berelated to two additional Arabidopsis AP2/EREBP genes, G1421 and G1755.The function of G864 was analyzed using transgenic plants in which thisgene was expressed under the control of the 35S promoter. G864overexpressing plants exhibited a variety of phenotypic alterations.They were smaller than wild-type plants, and those with the strongestphenotypes were classified as dwarf. However, G864 overexpressing linesshowed more seedling vigor in a heat stress tolerance germination assaycompared to wild-type controls. Conversely, G864 overexpressing lineswere also somewhat more sensitive to chilling. One of the three T2 linesanalyzed showed significant increase in fucose and arabinose levels inleaves.

G864 was ubiquitously expressed, and was not significantly induced underany of the conditions tested.

Potential Applications

The germination of many crops is very sensitive to temperature. A genethat would enhance germination in hot conditions such as G864 or itsequivalogs would be useful for crops that are planted late in the seasonor in hot climates.

G867 (SEQ ID NO: 169)

Published Information

G867 corresponds to RAV1 (Kagaya et al. (1999) Nucleic Acids Res. 27:470-478). G867/RAV1 belongs to a small subgroup within the AP2/EREBPfamily of transcription factors, whose distinguishing characteristic isthat its members contain a second DNA-binding domain, in addition to theconserved AP2 domain, that is related to the B3 domain of VP1/ABI3(Kagaya et al. (1999) supra). It has been shown that the two DNA-bindingdomains of RAV1 can separately recognize each of two motifs thatconstitute a bipartite binding sequence and together cooperativelyenhance its DNA-binding affinity and specificity (Kagaya et al. (1999)supra).

Experimental Observations

G867 was discovered and initially identified as a public ArabidopsisEST. G867 appeared to be constitutively expressed at medium levels.

G867 was first characterized using a line that contained a T-DNAinsertion in the gene. The insertion in that line resided immediatelydownstream of the conserved AP2 domain, and would therefore be expectedto result in a severe or null mutation. G867 knockout mutant plants didnot show significant changes in overall plant morphology, significantdifferences between these plants and control plants have not beendetected in any of the assays that have been performed so far.

Subsequently, the function of G867 was analyzed using transgenic plantsin which this gene was expressed under the control of the 35S promoter.G867 overexpressing lines were morphologically wild-type and nophenotypic alterations in G867 overexpressing lines were detected in thebiochemical assays that were performed. However, G867 overexpressinglines showed increased seedling vigor (manifested by increased expansionof the cotyledons) in germination assays on both high salt and highsucrose containing media, compared to wild-type controls.

The Arabidopsis paralogs G1930 (SEQ ID NO: 369) and G9 (SEQ ID NO: 1949)also showed stress related phenotypes. G9 exhibited increased rootbiomass, and thus could be used to produce better plant growth underadverse osmotic conditions. Genetic and physiological evidence indicatesthat roots subjected to various stresses, including water deficit, alterthe export of specific compounds, such as ACC and ABA, to the shoot, viathe xylem Bradford et al. (1980) Plant Physiol. 65: 322-326; Schurr etal. (1992) Plant Cell Environ. 15, 561-567).

G1930 plants responded to high NaCl and high sucrose on plates with moreseedling vigor, and root biomass compared to wild-type control plants;this phenotype was identical to that seen in 35S::G867 lines. Theseresults indicate a general involvement of this clade in abiotic stressresponses:

The polypeptide sequences of G1930 and G9 share 72% (249/345 residues)and 64% (233/364 residues) with G867, respectively. The conserveddomains of G1930 and G9 are 86% (56/65 residues) and 86% (56/65residues) identical with the conserved domain of G867, respectively.

In addition to the paralogous sequences disclosed above, orthologoussequences from other plant species were also identified using BLASTanalysis. Such orthologous sequences, together with the paralogoussequences were determined to be members of the G867 TF family of AP2proteins (equivalogs). The paralogous sequences and the orthologoussequences were aligned using MACVECTOR software (Accelrys, Inc.). Thesoftware program also generated an exemplary consensus amino acidresidue sequence of the aligned sequences.

As shown in FIGS. 4A, 4B, 4C, and 4D, the orthologous sequences shared aconsensus sequence with the conserved domain of G867 (amino acidresidues 59-116 of SEQ ID NO:170) and also shared identity with regionsflanking the conserved domain (flanking regions). In particular, G867shared a region of the conserved domain with sequences from soy (Glycinemax; SEQ ID NOs: 1184, 1183, and 1182), rice (Oryza sativa; SEQ ID NOs:1176, 1177, and 1178), and maize (corn) (Zea mays; SEQ ID NOs: 1186 and1185).

An exemplary consensus of the conserved domain of the G867 TF family ofAP2 proteins isSer-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Glu/Asp-Glu-Glu/Asp-Glu/Asp-Ala-Ala/Val-Arg-Ala/Ser-Tyr-Asp-Val/Ile-Ala/Val-Val/Ala-Xaa-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn-Phe-Lys/Arg,where Xaa is any amino acid residue. An alternative exemplary consensusof the conserved domain isSer-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Glu/Asp-Glu-Glu/Asp-Ala-Ala-Ala-Arg-Ala-Tyr-Asp-Val/Ile-Ala/Val-Val/Ala-Xaa-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn,where Xaa is any amino acid residue. A further alternative exemplaryconsensus of the conserved domain isSer-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Gly-Glu-Ala/Asp-Glu/Asp-Ala-Ala/Val-Arg-Ala-Tyr-Asp-Val-Ala-Ala-Gln-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn-Phe-Arg,where Xaa is any amino acid residue.

Potential Applications

G867 or its equivalogs could be used to increase or facilitate seedgermination and seedling growth under adverse environmental conditions,in particular salt stress.

G867 or its equivalogs may also be used to modify sugar sensing.

G869 (SEQ ID NO: 171)

Published Information

A partial cDNA sequence of G869 is available as public ESTs N65486. Thesequence of G869 later appeared among the Arabidopsis sequences releasedby the Arabidopsis Genome Initiative, in BAC T26J14 (GenBank accessionnumber AC011915).

Experimental Observations

The complete cDNA sequence of G869 was determined. The function of thisgene was analyzed using transgenic plants in which G869 was expressedunder the control of the 35S promoter. Plants overexpressing G869 weresmall with spindly bolts. G869 transgenic plants showed alterations inleaf and seed fatty acid composition. In leaves, 16:0 levels decreasedand 16:3 levels increased. These changes likely reflected alterations inthe desaturation state of chloroplast membranes. In seeds, 18:1 levelsincreased significantly. The increase in the seed 18:1 fatty acid in twolines was observed in a repeat experiment. A decrease in 18:3 and 20:0was also noted in these lines.

Alterations in the levels of leaf insoluble sugars were also detected,with the increase in fucose determined to be significant. In addition,G869 overexpressors were more tolerant to infection with a moderate doseof the fungal pathogen Erysiphe orontii. The increase in resistancephenotype co-segregated with the dwarf phenotype. G869 plants showedadditional morphological alterations, including poor fertility due tounderdeveloped anthers.

Potential Applications

G869 or its equivalogs could be useful to manipulate the saturationlevels of lipids in seeds. Alteration in seed lipid saturation could beused to improve the heat stability of oils or to improve the nutritionalquality of seed oil.

As G869 transgenic plants have an altered response to the fungalpathogen Erysiphe orontii, G869 or its equivalogs could be used tomanipulate the defense response in order to generate pathogen-resistantplants.

G877 (SEQ ID NO: 173)

Published Information

G877 was identified in an Arabidopsis EST (N37131). G877 is contained inP1 clone MXK23 (GenBank accession number AB026656).

Closely Related Genes from Other Species

A non-Arabidopsis gene closely related to G877 is the tobacco geneNtWRKY4 (GenBank accession number AB026890). Similarity between thesetwo genes extends beyond the conserved WRKY domain.

Experimental Observations

G877 was first discovered and identified as a public Arabidopsis EST.The complete sequence of G877 was determined.

A line was identified that contains a T-DNA insertion in the codingsequence of G877. The insertion likely resulted in a null mutation,since it resided upstream of the conserved WRKY domain sequence. Plantsthat were hemizygous for that insertion segregate 3 viable: 1 inviableseeds in the silique, and homozygous G877 knockout mutant plants werenever obtained. Therefore, a (null) mutation in G877 results in embryolethality.

G877 was ubiquitously expressed. G877 is likely to be involved incontrolling some essential process(es) required for growth rather thanspecific aspects of embryo patterning and development. Alternatively,G877 might play different roles throughout the plant life cycle.

Potential Applications

The embryo lethal phenotype of a G877 mutation indicates that the geneis involved in the control of some essential aspect of growth anddevelopment. G877 or its equivalogs could therefore constitute anherbicide target, either by itself or by allowing the identification ofother genes or processes essential for plant growth.

G881 (SEQ ID NO: 175)

Published Information

G881 corresponds to gene F28M20.10, first identified in the sequence ofBAC clone F28M20 (released by the Arabidopsis Genome Initiative; GenBankaccession number AL031004).

Experimental Observations

The complete cDNA sequence for G881 was determined. The annotation inGenBank for this gene (BAC AL031004) was found to be inaccurate. G881was ubiquitously expressed, but appeared to be significantly induced inresponse to salicylic acid treatment. The function of this gene wasanalyzed using transgenic plants in which G881 was expressed under thecontrol of the 35S promoter. G881 overexpressors appeared to be moresusceptible to infection with a moderate dose of the fungal pathogenErysiphe orontii. Increased susceptibility to Erysiphe orontii wasconfirmed in repeat experiment. The induction of G881 expression by SAalso implicated G881 in the disease response.

Potential Applications

Since G881 transgenic plants appear to have an altered response to thefungal pathogen Erysiphe orontii, G881 or its equivalogs could be usedto manipulate the defense response in order to generatepathogen-resistant plants.

G892 (SEQ ID NO: 177)

Published Information

G892 was identified in the sequence of BAC clone T13D8, GenBankaccession number AC004473, released by the Arabidopsis GenomeInitiative.

Experimental Observations

The complete sequence of G892 was determined A line homozygous for aT-DNA insertion in G892 was used to determine the function of this gene.The T-DNA insertion of G892 was approximately 70% into the codingsequence of the gene and therefore was likely to result in a nullmutation. The phenotype of these transgenic plants was wild-type in allassays performed. G892 appeared to be constitutively expressed at low ormoderate levels in all tissues except in roots where expression was muchhigher. ABA or salt treatment caused a slight increase in the expressionof G892.

G892 knockout mutants were found to have increased seed oil anddecreased protein content compared to wild-type plants.

Potential Applications

G892 or its equivalogs may be used to alter seed oil and protein contentin plants, which may be very important for the nutritional value andproduction of various food products.

G896 (SEQ ID NO: 179)

Closely Related Genes from Other Species

G896 is very similar to a peppermint EST (AW255156). Since the homologyextends beyond the conserved domain, G896 and the mint gene are likelyorthologs.

Experimental Observations

A knock-out mutant was isolated, which contains a T-DNA insertion 40base pairs downstream of the start codon. G896 knock-out plants weremore susceptible to Fusarium oxysporum. In addition, G896 knockoutplants had lower levels of lutein in seeds as compared to wild-typecontrol plants. Otherwise, the knock-out plants had a wild-typemorphological phenotype.

In wild-type plants, G896 was mostly expressed in roots. Changes inenvironmental conditions did not affect its expression.

Potential Applications

Since G896 transgenic plants have an altered response to the fungalpathogen Fusarium oxysporum, the gene or its equivalogs could be used tomanipulate the defense response in order to generate pathogen-resistantplants.

G910 (SEQ ID NO: 181)

Published Information

G910 was identified as a gene in the sequence of BAC T22E19 (AccessionNumber AC016447), released by The Institute for Genomic Research.

Experimental Observations

The function of G910 was analyzed using transgenic plants in which G910was expressed under the control of the 35S promoter.

G910 overexpression produced pleiotropic effects on plant development,but the most marked result was a delay in the transition to flowering.At early stages, 35S::G910 T1 lines appeared normal, but by around 20days after sowing, most plants were clearly smaller than wild type andoften had contorted or serrated leaves with short petioles.Approximately half of the T1 lines bolted at a normal time and producedrather thin inflorescences that yielded relatively few seeds. Theremaining half of the T1 lines typically produced flowers between fiveand 30 days later than wild type. Although such late flowering plantsinitially appeared small, in many cases, by the time of bolting, theyhad attained a similar size and produced a much larger number of primaryrosette leaves than controls. Two T2 lines showed a similar, but moreextreme late flowering phenotype.

G910 was expressed at low levels in shoots and germinating seedlings,and at higher levels in flowers, rosette leaves, embryonic tissue andsiliques. The expression of G910 was repressed by cold treatment andinduced by NaCl treatment.

Potential Applications

Plants expressing G910 exhibited a delayed flowering time relative tocontrols. A wide variety of applications exist for genes or theirequivalogs that either lengthen or shorten the time to flowering.

G911 (SEQ ID NO: 183)

Closely Related Genes from Other Species

An EST (GenBank accession AI352907) induced in the defense response ofBrassica napus to Leptosphaeria maculans has extremely high homologyboth within and external to the conserved RING H2 domain.

Experimental Observations

The function of G911 was analyzed through its ectopic overexpression inArabidopsis. RT-PCR of endogenous levels of G911 indicated this gene wasexpressed in all tissues tested. A cDNA array experiment confirmed thistissue distribution data by RT-PCR. Microarray data confirmed that G911was overexpressed 23 fold. Other genes that were induced when G911 wasoverexpressed included RHA1b (another RING C2H3C2 transcription factor),pistilata, and a proline rich protein isolog. Plants overexpressing G911looked healthier and had longer roots when grown on media lackingpotassium compared to wild-type plants.

Potential Applications

Plants overexpressing G911 or its equivalogs may be able to be grownwith fertilizer lacking or containing low potassium.

G912 (SEQ ID NO: 185)

Published Information

G912 was identified in the sequence of P1 clone MSG15 (GenBank accessionnumber AB015478; gene MSG15.6).

Closely Related Genes from Other Species

G912 is closely related to CBF1, CBF2, and CBF3, and also closelyrelated to the members of the CBF-like subgroup of AP2/EREBP proteinsfrom other plants, like AF084185 Brassica napus dehydration responsiveelement binding protein.

Experimental Observations

G912 was recognized as the AP2/EREBP gene most closely related toArabidopsis CBF1, CBF2, and CBF3 (Stockinger et al (1997) Proc. Natl.Acad. Sci. USA 94:1035-1040; Gilmour et al. (1998) Plant J. 16:433-442).In fact, G912 is the only other AP2/EREBP transcription factor for whichsequence similarity with CBF1, CBF2, and CBF3 extends beyond theconserved AP2 domain.

The function of G912 was studied using transgenic plants in which thisgene was expressed under the control of the 35S promoter. Plantsoverexpressing G912 were more freezing and drought tolerant than thewild-type controls, but were also small, dark green, and late flowering.There was a positive correlation between the degree of growth impairmentand the freezing tolerance. In addition, G912 expression appeared to beinduced by cold, drought, and osmotic stress.

In addition, G912 overexpressing plants also exhibited a sugar sensingphenotype: reduced seedling vigor and cotyledon expansion upongermination on high glucose media.

These results mirror the extensive body of work that has shown thatCBF1, CBF2, and CBF3 are involved in the control of the low-temperatureresponse in Arabidopsis, and that those genes can be used to improvefreezing, drought, and salt tolerance in plants (Stockinger et al.,(1997) Proc. Natl. Acad. Sci. USA 94:1035-1040; Gilmour et al. (1998)Plant J. 16:433-442; Jaglo-Ottosen et al. (1998) Science. 280:104-106;Liu et al. (1998) Plant Cell. 10:1391-1406, Kasuga et al. (1999) Nat.Biotechnol. 17:287-291).

The polypeptide sequences of G40, G41, and G42 share 71% (140 of 195residues), 68% (144 of 211 residues), and 65% (147 of 224 residues)identity with G912, respectively. The conserved domains of G40, G41, andG42 share 94% (64 of 68 residues), 92% (63 of 68 residues), and 94% (64of 68 residues) identity with G912, respectively.

In addition to the paralogous sequences disclosed above, orthologoussequences from other plant species were also identified using BLASTanalysis. Such orthologous sequences, together with the paralogoussequences were determined to be members of the G912 TF family ofAP2/EREBP proteins (equivalogs). The paralogous sequences and theorthologous sequences were aligned using MACVECTOR software (Accelrys,Inc.). The software program also generated an exemplary consensus aminoacid residue sequence of the aligned sequences.

As shown in FIGS. 5A, 5B, 5C, 5D, 5E, and 5F, the orthologous sequencesshared a consensus sequence with the conserved domain of G912 (aminoacid residues 51-118 of SEQ ID NO:186) and also shared identity withregions flanking the conserved domain (flanking regions). In particular,G912 shared a region of the conserved domain with sequences from soy(Glycine max; SEQ ID NOs: 1238, 1242, 1240, 1241, and 1243), rice (Oryzasativa; SEQ ID NOs: 1222, 1223, 1232, 1221, 1231, 1227, 1235, 1230,1229, and 1228), and maize (corn) (Zea mays; SEQ ID NOs: 1246, 1247,1244, and 1245).

An exemplary consensus of the conserved domain of the G912 TF family ofAP2/EREBP proteins isHis-Pro-IleNal-Tyr/Phe-Arg/Lys-Gly-Val-Arg-Gln/Arg-Arg-Gly/Asn-Xaa₍₁₋₃₎-Lys/Arg-Trp-Val-Cys/Ser-Glu-Val/Leu-Arg-Glu/Val-Pro-Asn-Lys-Xaa₍₂₎-Arg-Ile/Leu-Trp-Leu-Gly-Thr-Phe/Tyr-Xaa₍₂₎-Ala/Pro-Glu-Met-Ala-Ala-Arg-Ala-His-Asp-Val-Ala-Ala/Met-Leu/Met-Ala-Leu-Arg-Gly-Xaa₍₁₋₈₎-Ala-Cys-Leu-Asn-Phe-Ala-Asp-Ser-Xaa₍₁₋₅₎-Val/Ile-Pro/Asp,where Xaa is any amino acid residue. An alternative exemplary consensusof the conserved domain isHis-Pro-Ile/Val-Tyr/Phe-Arg/Lys-Gly-Val-Arg-Xaa-Arg-Gly/Asn-Xaa₍₁₋₃₎-Lys/Arg-Trp-Val-Cys/Ser-Glu-Val/Leu-Arg-Glu/Val-Pro-Xaa₍₁₋₅₎-Arg-Ile/Leu/Phe-Trp-Leu-Gly-Thr-Phe/Tyr-Xaa₍₂₎-Ala/Pro-Glu-Xaa-Ala-Ala-Arg-Ala-His-Asp-Val-Ala-Ala/Met-Leu/Met-Ala-Leu-Arg-Gly-Xaa₍₁₋₈₎-Ala-Cys/Ser-Leu-Asn-Phe-Ala-Asp-Ser-Xaa₍₁₋₅₎-Val/Ile-Pro/Asp,where Xaa is any amino acid residue.

An exemplary flanking region consensus sequence of the G912 TF family ofAP2/EREBP proteins is Pro-Lys-Xaa-Xaa-Ala-Gly-Arg (amino acids 37-43 ofSEQ ID NO: 186), or Ala-Gly-Arg-Xaa-Lys-Phe (amino acids 41-46 of SEQ IDNO: 186) or Glu-Thr-Arg-His-Pro (amino acids 48-52 of SEQ ID NO: 186),where Xaa is any amino acid residue.

Potential Applications

G912 or its equivalogs could be used to improve plant tolerance to cold,freezing, drought, and salt stress. In addition, G912 or its equivalogscould be used to change a plant's flowering time and size.

G913 (SEQ ID NO: 187)

Published Information

G913 was identified in the sequence of clone MSG15; it corresponds togene MSG15.10 (GenBank PID BAB11050).

Closely Related Genes from Other Species

G913 is highly similar to a Brassica napus protein, encoded by a generepresented by EST AI352878 MB72-11D PZ204.BNlib Brassica napus cDNAclone pMB72-11D 5′.

Experimental Observations

The cDNA sequence of G913 was determined. To investigate the function(s)of G913, this gene was expressed under the control of the 35S promoterin transgenic plants. G913 overexpressing plants had dark green leavesthat occasionally curled downward. These plants showed a delay inflowering and were also late senescing. Overexpressing G913 lines weremore freezing tolerant and more drought tolerant than the wild-typecontrols.

In an ethylene sensitivity assay where the plants were tested for atriple response phenotype on plates containing ACC, G913 overexpressingplants showed stunting and curling in the hypocotyl region that was moreexaggerated than the wild type triple response.

Potential Applications

G913 or its equivalogs could be used to improve plant tolerance tofreezing and drought. G913 could also be used to manipulate the ethyleneresponse.

G913 or its equivalogs may be used to delay flowering or senescence inplants. Extending vegetative development could bring about largeincreases in yields.

Additionally, a major concern is the escape of transgenic pollen fromGMOs to wild species or so-called organic crops. Systems that preventvegetative transgenic crops from flowering would eliminate this worry.

G922 (SEQ ID NO: 189)

Published Information

G922 corresponds to Scarecrow-like 3 (SCL3) first described by Pysh etal. (GenBank accession number AF036301; (1999) Plant J. 18: 111-119).Northern blot analysis results show that G922 is expressed in siliques,roots, and to a lesser extent in shoot tissue from 14 day old seedlings.Pysh et al did not test any other tissues for G922 expression. In situhybridization results showed that G922 was expressed predominantly inthe endodermis in the root tissue. This pattern of expression was verysimilar to that of SCARECROW (SCR), G306. Experimental evidenceindicated that the co-localization of the expression is not due tocross-hybridization of the G922 probe with G306. Pysh et al proposedthat G922 may play a role in epidermal cell specification and that G922may either regulate or be regulated by G306.

The sequence for G922 can also be found in the annotated BAC cloneF11F12 from chromosome 1 (GenBank accession number AC012561). Thesequence for F11F12 was submitted to GenBank by the DNA Sequencing andTechnology Center at Stanford University.

Closely Related Genes from Other Species

The amino acid sequence for a region of the Oryza sativa chromosome Iclone P0466H10 (GenBank accession number AP003259) is significantlyidentical to G922 outside of the SCR conserved domains. Therefore, thegene represented by this region of the rice clone may be the ortholog ofG922.

Experimental Observations

The function of this gene was analyzed using transgenic plants in whichG922 was expressed under the control of the 35S promoter. Transgenicplants overexpressing G922 were more salt tolerant than wild-type plantsas determined by a root growth assay on MS media supplemented with 150mM NaCl. Plant overexpressing G922 also were more tolerant to osmoticstress as determined by germination assays in salt-containing (150 mMNaCl) and sucrose-containing (9.4%) media. Morphologically, plantsoverexpressing G922 had altered leaf morphology, coloration, fertility,and overall plant size. In wild-type plants, expression of G922 wasinduced by auxin, ABA, heat, and drought treatments. In non-inducedwild-type plants, G922 was expressed constitutively at low levels.

Potential Applications

Based upon results observed in plants overexpressing G922, G922 or itsequivalogs could be used to alter salt tolerance, tolerance to osmoticstress, and leaf morphology in other plant species. Evaporation from thesoil surface causes upward water movement and salt accumulation in theupper soil layer where the seeds are placed. Thus, germination normallytakes place at a salt concentration much higher than the mean saltconcentration of in the whole soil profile. Increased salt toleranceduring the germination stage of a crop plant would impact survivabilityand yield.

Altered leaf morphology could be desirable in ornamental horticulture.

G926 (SEQ ID NO: 191)

Published Information

G926 is equivalent to Hap2a (Y13720), a member of the CCAAT-box bindingtranscription factor family. The gene was identified by Edwards et al.((1998) Plant Physiol. 117: 1015-1022), who demonstrated that G926 orAtHap2a were able to functionally complement a Hap2 deficient mutant ofyeast suggesting that there is functional conservation between theseproteins from diverse organisms. In addition, the AtHap2a gene was shownto be ubiquitously expressed in Arabidopsis.

Closely Related Genes from Other Species

G926 is most closely related to a Brassica napus protein (AAC49265).Similarity between the two proteins extend beyond the signature motif ofthe family to a level that would indicate the genes are orthologous. Nofunctional information is available for the Brassica napus protein.

Experimental Observations

Consistent with the published expression pattern (Edwards et al. (1998)Plant Physiol. 117: 1015-1022), G926 was determined to be ubiquitouslyexpressed and transcript levels appeared to be unaltered by anyenvironmental stress-related condition tested. A line homozygous for aT-DNA insertion in G926 was used to determine the function of this gene.

The G926 knockout mutant line was morphologically wild-type.Physiological analysis revealed that in the presumed absence of G926function, the plants became more tolerant to high osmotic conditionsduring germination. This osmotic stress tolerance could be related tothe plant's apparent insensitivity to the growth hormone ABA. This wasthe second instance where a member of a CCAAT-box protein complexaltered the plants osmotic stress response and ABA sensitivity duringgermination.

ABA plays an important regulatory role in the initiation and maintenanceof seed dormancy. Lopez-Molina, L. et al. ((2001) Proc. Natl. Acad. Sci.USA 98: 4782-4787) describe a bZIP transcription factor, ABI5, that isinvolved in maintaining seeds in a quiescent state, preventinggermination under adverse conditions such as drought stress. It ispossible G926 also functions as part of this checkpoint for thegerminating seeds and loss of G926 function promotes germinationregardless of the osmotic status of the environment.

Potential Applications

G926 or its equivalogs could be used to improve plant tolerance todrought, and salt stress.

Evaporation from the soil surface causes upward water movement and saltaccumulation in the upper soil layer where the seeds are placed. Thus,germination normally takes place at a salt concentration much higherthan the mean salt concentration of in the whole soil profile. Increasedsalt tolerance during the germination stage of a crop plant would impactsurvivability and yield.

G961 (SEQ ID NO: 193)

Published Information

G961 was first identified in the sequence of the BAC clone F19D11,GenBank accession number AC005310, released by the Arabidopsis GenomeInitiative.

Closely Related Genes from Other Species

The most related gene to G961 is a rice gene in accession numberBAA84803.

Experimental Observations

The full length sequence of G961 was experimentally confirmed. Thefunction of this gene was analyzed by knockout analysis. Plantshomozygous for a T-DNA insertion in G961 were wild-type for all assaysperformed.

Gene expression profiling by RT-PCR showed that G961 was primarilyexpressed in shoots, embryos and siliques at medium levels, and at lowlevels in flowers. RT-PCR data also indicated an induction of G961transcripts accumulation upon heat treatment.

G961 knockout mutants were found to have altered seed oil content ascompared to wild-type plants.

Potential Applications

G961 or its equivalog knockout mutants may be used to alter seed oilcontent in plants, which may be very important for the nutritional valueand production of various food products.

G971 (SEQ ID NO: 195)

Published Information

G971 corresponds to gene F28P10.30 (CAB41085).

Experimental Observations

The function of G971 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter.

Overexpression of G971 produced a marked delay in the transition toflowering. The effect was noted, to varying extents, in approximatelyhalf of the 35S::G971 primary transformants. These plants floweredbetween one and three weeks later than controls under continuous lightconditions. At later stages, most of the plants also appeared darkergreen and developed larger leaves than controls. Two of the three T2populations selected for further study displayed a comparable, butrather more extreme late flowering phenotype to that seen in theparental plants. At early stages, seedlings from these two lines wererelatively small, but recovered as development progressed, andeventually became larger than wild type. No alterations were detected in35S::G971 plants in the physiological and biochemical analyses that wereperformed.

G971 was ubiquitously expressed and does not appear to be significantlyinduced by any of the conditions tested.

Potential Applications

G971 or its equivalogs could be used to modify flowering timecharacteristics. A wide variety of applications exist for systems thateither lengthen or shorten the time to flowering.

In species such as sugarbeet where the vegetative parts of the plantsconstitute the crop and the reproductive tissues are discarded, it wouldbe advantageous to delay or prevent flowering. Extending vegetativedevelopment could bring about large increases in yields.

G974 (SEQ ID NO: 197)

Published Information

G974 was first identified in a BAC-end sequence (B28553; partial G974sequence). G974 corresponds to gene F16L1.8 (BAC F16L1, AC024228).

Closely Related Genes from Other Species

Several AP2 proteins from a variety of species (Atriplex hortensis,Lycopersicon esculentum, Glycine max, Populus balsamifera, Medicagotruncatula) exhibited sequence similarity with G974 outside of thesignature AP2 domain sequence, and bear nearly identical AP2 domains.These proteins may be related.

Experimental Observations

The complete sequence of G974 (SEQ ID NO: 197) was obtained and G974 wasstudied using transgenic plants in which G974 was expressed under thecontrol of the 35S promoter. Constitutive expression of G974 produceddeleterious effects: the majority of 35S::G974 primary transformantsshowed a reduction in overall size and developed rather slowly comparedto wild-type controls. These phenotypic alterations were not observed inthe T2 generation, perhaps indicating silencing of the transgene. The T2plants were wild-type in the physiological and biochemical analysesperformed. G974 was ubiquitously expressed.

35S::G974 overexpressors had altered seed oil content.

Potential Applications

G974 or its equivalogs may be used to alter seed oil content in plants,which may be very important for the nutritional value and production ofvarious food products.

G975 (SEQ ID NO: 199)

Published Information

G975 has appeared in the sequences released by the Arabidopsis GenomeInitiative (BAC F9L1, GenBank accession number AC007591).

Closely Related Genes from Other Species

The non-Arabidopsis gene most highly related to G975 is represented byL46408 BNAF1258 Mustard flower buds Brassica rapa cDNA clone F1258. Thesimilarity between G975 and the Brassica rapa gene represented by ESTL46408 extends beyond the conserved AP2 domain that characterizes theAP2/EREBP family. This Brassica rapa gene appeared to be more closelyrelated to G975 than Arabidopsis G1387, indicating that EST L46408 mayrepresent a true G975 ortholog. The similarity between G975 andArabidopsis G1387 also extends beyond the conserved AP2 domain.

Experimental Observations

G975 (SEQ ID NO: 199) was identified as a new member of the AP2/EREBPfamily (EREBP subfamily) of transcription factors. G975 was expressed inflowers and, at lower levels, in shoots, leaves, and siliques. GC-FIDand GC-MS analyses of leaves from G975 overexpressing plants showed thatthe levels of C29, C31, and C33 alkanes were substantially increased (upto ten-fold) compared with control plants. A number of additionalcompounds of similar molecular weight, presumably also wax components,also accumulated to significantly higher levels in G975 overexpressingplants. C29 alkanes constituted close to 50% of the wax content inwild-type plants (Millar et al. (1998) Plant Cell 11:1889-1902),suggesting that a major increase in total wax content occurred in theG975 transgenic plants. However, the transgenic plants had an almostnormal phenotype (although small morphological differences were detectedin leaf appearance), indicating that overexpression of G975 was notdeleterious to the plant. Overexpression of G975 did not cause thedramatic alterations in plant morphology that had been reported forArabidopsis plants in which the FATTY ACID ELONGATION1 gene wasoverexpressed (Millar et al. 1998, Plant Cell 11:1889-1902). G975 mayregulate the expression of some of the genes involved in wax metabolism.One Arabidopsis AP2 sequence (G1387) that is significantly more closelyrelated to G975 than the rest of the members of the AP2/EREBP family ispredicted to have a function and a use related to that of G975.

Potential Applications

G975 or its equivalogs can be used to manipulate wax composition,amount, or distribution, which in turn can modify plant tolerance todrought and/or low humidity or resistance to insects, as well as plantappearance (shiny leaves).

G975 or its equivalogs can also be used to specifically alter waxcomposition, amount, or distribution in those plants and crops fromwhich wax is a valuable product.

G979 (SEQ ID NO: 201)

Published Information

G979 was first identified in a BAC-end sequence (B25031; partial G979sequence). G979 corresponds to gene T12E18_(—)20 (BAC T12E18, AL132971).No information is available about the function(s) of G979.

Experimental Observations

The complete sequence of G979 was obtained. The function of this genewas studied using both transgenic plants in which G979 was expressedunder the control of the 35S promoter (April 2001), and a line with aT-DNA insertion in the gene. G979 codes for an AP2 protein of the AP2subfamily, i.e., it contains two AP2 domains. The T-DNA insertion of theKO line lies in an intron, located in between the exons coding for thesecond AP2 domain of the protein, and is thus expected to result in astrong or null mutation. Whereas constitutive expression of G979produced deleterious effects, the analysis of G979 KO mutant plantsproved informative about the function of the gene. It was suggested thatproteins of the AP2 subfamily were more likely to be involved indevelopmental processes (Riechmann et al. (1998). Biol. Chem. 379:633-646). Fittingly, seeds homozygous for a T-DNA insertion within G979showed delayed ripening, slow germination, and developed into small,poorly fertile plants, indicating that G979 is involved in seeddevelopment processes.

The difficulty in initially isolating, from heterozygous plants, progenythat was homozygous for the T-DNA insertion raised the possibility thathomozygosity for that allele was lethal. Siliques of heterozygous plantswere examined for seed abnormalities. Approximately 25% of the seedscontained in young green siliques were pale in coloration. In older,brown siliques, approximately 25% of the seeds were green and appearedslow ripening, whereas the remaining seeds were brown. Thus, it seemedlikely that the seeds with altered development were homozygous for theT-DNA insertion, whereas the normal seeds were wild-type andheterozygous segregants.

Furthermore, it was observed that approximately 25% of the seed fromG979 knockout heterozygous plants showed impaired (delayed) germination.Upon germination, these seeds produced extremely tiny seedlings thatoften did not survive transplantation. A few small and sickly lookinghomozygous plants could be grown, which produced siliques that containedseeds that were small and wrinkled compared to wild type.

A second, different, T-DNA insertion allele for G979 was identified aspart of a TAIL PCR screen. Progeny of the heterozygous plant carryingthat T-DNA insertion was either wild-type or heterozygous for themutation, providing additional evidence for the disruption of G979 beingthe cause of the phenotypic alterations detected.

The initial analysis of the gene was performed using overexpressinglines. 35S::G979 transformants were generally smaller than wild type anddeveloped spindly inflorescences that carried abnormal flowers withcompromised fertility.

G979 expression was ubiquitous and not induced under any of theconditions tested.

Potential Applications

On the basis of the results obtained with G979 knockout mutant lines, itis possible that G979 or its equivalogs could be used to alter or modifyseed germination, ripening and development properties and performance.

G987 (SEQ ID NO: 203)

Published Information

The genomic sequence of G987 is located on the Arabidopsis BAC cloneT9I4 (gene T9I4.14) (GenBank accession number AC005315).

Experimental Observations

As determined by RT-PCR analysis, G987 was constitutively expressed inall tissues tested. A line homozygous for a T-DNA insertion in G987 wasused to determine the function of this gene. The T-DNA insertion in G987was approximately 4% into the coding sequence of the gene, and thereforeis likely to result in a null mutation. G987 mutant plants could only begrown on sucrose-containing medium. Biochemical analyses of leaves fromG987 mutants grown on sucrose-containing medium indicate that themutants had reduced amounts of 16:3 fatty acids, the presence of twoxanthophylls which were not present in wild-type leaves, the presence ofgamma-tocopherol (which normally accumulates in seed tissue), andreduced levels of chlorophyll a and chlorophyll b.

Potential Applications

The low amount of 16:3 and dramatic reduction in chlorophyll indicatedthat the gene controls some aspect of thylakoid membrane development.G987 or its equivalogs may control proplastid to chloroplastdevelopment. This could be tested by measuring the expression of some ofthe genes (e.g. LHCP) that are associated with the transition fromproplastid to chloroplast. If this were the case, the gene or itsequivalogs may be useful for controlling the transition from proplastidto chromoplast in fruits and vegetables. There may also be someapplications where it would be desirable to change the expression of thegene or its equivalogs (e.g., prevent cotyledon greening in Brassicanapus or campestris to avoid green oil due to early frost).

G988 (SEQ ID NO: 205)

Published Information

G988 corresponds to a protein annotated as hypothetical in BAC F20N2(GenBank accession number AC002328) from chromosome 1 of Arabidopsis.The sequence for G988 can also be found on the chromosome 1 BAC cloneT5A14 and is described in patent application WO 98/46759.

Closely Related Genes from Other Species

The amino acid sequence for the Capsella rubella hypothetical proteinrepresented by GenBank accession number CRU303349 was significantlyidentical to G988 outside of the SCR conserved domains. The Capsellarubella hypothetical protein is 90% identical to G988 over a stretch ofroughly 450 amino acids. Therefore, it is likely that the Capsellarubella gene is an ortholog of G988.

Experimental Observations

G988 (SEQ ID NO: 205) was analyzed using transgenic plants in which G988was expressed under the control of the 35S promoter. Plantsoverexpressing G988 had multiple morphological phenotypes. Thetransgenic plants were generally smaller than wild-type plants, hadaltered leaf, inflorescence and flower development, altered plantarchitecture, and altered vasculature.

Plants overexpressing G988 were found to have decreased seed oil andincreased seed protein. In one transgenic line overexpressing G988, anincrease in the seed glucosinolate M39489 was observed.

In wild-type plants, G988 was expressed primarily in flower and siliquetissue, but was also present at detectable levels in all other tissuestested. Expression of G988 was induced in response to heat treatment,and repressed in response to infection with Erysiphe.

Potential Applications

Based on the observed morphological phenotypes of the transgenic plants,G988 or its equivalogs can be used to create plants with larger flowers.This can have value in the ornamental horticulture industry. Thereduction in the formation of lateral branches suggests that G988 canhave utility on the forestry industry. The Arabidopsis plantsoverexpressing G988 also had reduced fertility. This could actually be adesirable trait in some instances, as it can be exploited to prevent orminimize the escape of GMO (genetically modified organism) pollen intothe environment.

G988 may also be used to modify seed oil and protein content.

G1040 (SEQ ID NO: 207)

Published Information

G1040 was identified in the sequence of BAC MF020, GenBank accessionnumber AB013391, released by the Arabidopsis Genome Initiative. G1040has been published as KAN4, one of a clade of four KANADI genes that arethought to promote abaxial cell fates in lateral organs (Eshed et al.(2001) Current Biology 11: 1251-1260).

Experimental Observations

A full-length cDNA corresponding to G1040 was isolated. The function ofthis gene was analyzed using transgenic plants in which G1040 wasexpressed under the control of the 35S promoter. Plants overexpressingG1040 were found to produce seeds that were generally smaller and morerounded than control seeds, with a high proportion of irregularly-shapedseeds. No other morphological, physiological, or biochemical alterationswere observed in these plants. G1040 may affect embryo development.G1040 was expressed throughout the plant, though at lower levels inshoots and rosette leaves than in other tissues.

Potential Applications

G1040 or its equivalogs could be used to manipulate seed size and shape.

G1047 (SEQ ID NO: 209)

Published Information

G1047 was identified in the sequence of BAC T20K9, GenBank accessionnumber AC004786, released by the Arabidopsis Genome Initiative.

Experimental Observations

The boundaries of G1047 were experimentally determined and the functionof G1047 was analyzed using transgenic plants in which this gene wasexpressed under the control of the 35S promoter. G1047 overexpressinglines were more tolerant to infection with a moderate dose of the fungalpathogen Fusarium oxysporum. G1047 overexpression did not seem to haveconsistent a detrimental effect on plant growth or vigor, and the linestested for resistance were reported as being wild-type morphologically.In addition, no difference was detected between those lines and thecorresponding wild-type controls in all the biochemical assays that wereperformed.

G1047 was ubiquitously expressed, and it was not significantly inducedunder any of the conditions tested

Potential Applications

G1047 transgenic plants have an altered response to the fungal pathogenFusarium oxysporum. Therefore, G1047 or its equivalogs could be used tomanipulate the defense response in order to generate pathogen-resistantplants.

G1051 (SEQ ID NO: 211)

Published Information

G1051 was initially identified in the sequence of BAC-end B77139 andsubsequently the entire sequence of G1051 was disclosed in the sequenceof BAC accession number AC005956, released by the Arabidopsis genomeinitiative.

Closely Related Genes from Other Species

G1051 is very similar to a rice bZIP transcription factor, accessionnumber BAA96162, identified as part of the rice genome sequencingproject. Homology between G1051 and this rice protein continues beyondthe conserved domain, suggesting that they are orthologous.

Experimental Observations

The boundaries of G1051 were experimentally determined and the functionof this gene was analyzed using transgenic plants in which G1051 wasexpressed under the control of the 35S promoter. Plants overexpressingG1051 exhibited a delay in flowering and typically produced flower budsabout one week later than controls in continuous light conditions. G1051was constitutively expressed throughout the plant and not induced by anycondition tested.

Potential Applications

G1051 or its equivalogs could be used to manipulate flowering time inplants

G1052 (SEQ ID NO: 213)

Published Information

G1052 was identified in the sequence of BAC F9D24, GenBank accessionnumber AL137081, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G1052 is similar to a rice gene BAA96162. Homology between G1052 and therice gene extends beyond the conserved domain, thus the two genes may beorthologous.

Experimental Observations

The boundaries of G1052 in BAC AL137081 were experimentally determinedand the function of G1052 was analyzed using transgenic plants in whichthis gene was expressed under the control of the 35S promoter. Plantsoverexpressing G1052 exhibited a delay in flowering and typicallyproduced flower buds about one week later than controls in continuouslight conditions. Additionally, these plants had larger leaves and weregenerally more sturdy than wild type.

A line homozygous for a T-DNA insertion in G1052 was also used todetermine the function of this gene. The T-DNA insertion of G1052 wasapproximately one third of the way into the coding sequence of the geneand therefore is likely to result in a null mutation. A decrease in thepercentage of lutein and increase in the xanthophyll 1 fraction wasdetected in one line in two experiments.

Potential Applications

The flowering time phenotype associated with G1052 over-expressionindicates a utility for G1052 or its equivalogs as genes that can beused to manipulate flowering time in commercial plants. In addition, ifthe G1052 can not be transmitted through pollen, G1052 or its equivalogsmay be used as a tool for preventing transgenes from escaping fromtransgenic plants through pollen dispersal.

G1052 or its equivalogs could be used to manipulate seed prenyl lipidcomposition. Lutein is an important nutraceutical, since lutein-richdiets have been shown to help prevent age-related macular degeneration(ARMD), which is the leading cause of blindness in people over the ageof 65. In particular, consumption of dark green leafy vegetables hasbeen shown in clinical studies to reduce the risk of ARMD. In addition,lutein, like other xanthophylls such as zeaxanthin and violaxanthin, isan essential component in the protection of the plant against thedamaging effects of excessive light. Specifically, lutein contributes,directly or indirectly, to the rapid rise of nonphotochemical quenchingin plants exposed to high light. Crop plants engineered to containhigher levels of lutein could therefore have improved photoprotection,possibly leading to less oxidative damage and better growth under highlight.

G1062 (SEQ ID NO: 215)

Published Information

G1062 corresponds to gene MLJ15.14 (BAB01738.1).

Closely Related Genes from Other Species

G1062 protein shares extensive homology in the basic helix loop helixregion with a cDNA from developing stem Medicago truncatula (AW691174)as well as a tomato shoot/meristem Lycopersicon esculentum cDNA(BG123327).

Experimental Observations

G1062 is a proprietary sequence initially identified from a libraryclone. The function of G1062 was analyzed by knockout analysis. TheT-DNA insertion of G1062 was approximately 75% into the coding sequenceof the gene and therefore is likely to result in a null mutation.

Homozygotes for a T-DNA insertion in G1062 showed slow growth andproduced abnormal seeds. Knockout.G1062 plants displayed a longer leafplastochron than wild type. Both generated flower buds at the same time,but wild-type plants had produced 9-11 rosette leaves at that point,compared to only 5-9 rosette leaves in the mutant (24 hour light).Following bolting, KO.G1062 inflorescences developed more slowly andwere shorter than wild type. Knockout G1062 seeds appeared twisted andwrinkled in comparison to wild-type seed.

Physiological assays revealed that seedlings from a G1062 knockoutmutant line have a light grown phenotype in the dark and were moreseverely stunted in an ethylene insensitivity assay when compared to thewild-type controls. This result indicated that G1062 may be involved inthe ethylene triple response pathway. It is well known that ethylene isinvolved in the seed ripening process and therefore, the abnormal seedphenotype could be related to a general sensitivity to ethylene signaltransduction pathway.

RT-PCR analysis indicated that the transcripts of G1062 werepredominantly accumulated in the reproductive tissues. Its expressionlevel appeared to be not affected by any treatments tested.

Potential Applications

G1062 or its equivalogs that alter seed shape are likely to provideornamental applications.

Since G1062 is involved in the ethylene triple response pathway, G1062could be used to manipulate seed or fruit ripening process, and toimprove seed or fruit quality.

G1063 (SEQ ID NO: 217)

Published Information

G1063 corresponds to gene K21H1.2 (BAB10940.1).

Closely Related Genes from Other Species

G1063 protein shared extensive homology in the basic helix loop helixregion with a protein sequence encoded by Glycine max cDNA clone(AW832545) as well as a tomato root, plants pre-anthesis Lycopersiconsculentum cDNA (BE451174).

Experimental Observations

G1063 (SEQ ID NO: 217) is a member of a clade of highly related HLH/MYCproteins that also includes G779, G1499, G2143, and G2557. All of thesegenes caused similar pleiotropic phenotypic effects when overexpressed,the most striking of which was the production of ectopic carpelloidtissue. These genes can be considered key regulators of carpeldevelopment. A spectrum of developmental alterations was observedamongst 35S::G1063 primary transformants and the majority were markedlysmall, dark green, and had narrow curled leaves. The most severelyaffected individuals were completely sterile and formed highly abnormalinflorescences; shoots often terminated in pin-like structures, andflowers were replaced by filamentous carpelloid structures. In othercases, flowers showed internode elongation between floral whorls, with acentral carpel protruding on a pedicel-like organ. Additionally, lateralbranches sometimes failed to develop and tiny patches of carpelloidtissue formed at axillary nodes of the inflorescence. In lines with anintermediate phenotype, flowers contained defined whorls of organs, butsepals were converted to carpelloid structures or displayed patches ofcarpelloid tissue. In contrast, lines with a weak phenotype developedrelatively normal flowers and produced a reasonable quantity of seed.Such plants were still distinctly smaller than wild-type controls. Sincethe strongest 35S::G1063 lines were sterile, three lines with arelatively weak phenotype, that had produced sufficient seed forbiochemical and physiological analysis, were selected for further study.Two of the T2 populations (T2-28,37) were clearly small, darker greenand possessed narrow leaves compared to wild type. Plants from one ofthese populations (T2-28) also produced occasional branches withabnormal flowers like those seen in the T1. The final T2 population(T2-30) displayed a very mild phenotype. Overexpression of G1063 inArabidopsis resulted in a decrease in seed oil content in two T2 lines.No altered phenotypes were detected in any of the physiological assays,except that the plants were noted to be somewhat small and produceanthocyanin when grown in Petri plates. G1063 was expressed at low tomoderate levels in roots, flowers, rosette leaves, embryos, andgerminating seeds, but was not detected in shoots or siliques. G1063 wasinduced by auxin

Potential Applications

G1063 or its equivalogs can be used to manipulate flower form andstructure or plant fertility. One application for manipulation of flowerstructure can be in the production of saffron, which is derived from thestigmas of Crocus sativus. G1063 has utility in manipulating seed oiland protein content.

G1064 (SEQ ID NO: 219)

Closely Related Genes from Other Species

G1064 protein shares a close homology to an auxin-induced basichelix-loop-helix transcription factor from Gossypium hirsutum(PID:5731257) in the bHLH motif region as well as outside of thisregion. G1064 also has high similarity to a tomato germinating seedlingscDNA clone (AW649873).

Experimental Observations

G1064 was initially identified from a library clone collection. Thefunction of G1064 was analyzed using transgenic plants in which G1064was expressed under the control of the 35S promoter.

Physiological assays revealed that G1064 overexpressing lines were moresusceptible to infection with a low dose of the fungal pathogen Botrytiscinerea compared to the wild-type controls.

No morphological and biochemical alterations were observed in theoverexpressing transgenic plants when compared to wild-type controls.Furthermore, RT-PCR analyses of the endogenous levels of G1064 indicatedthat this gene was uniformly expressed in all tissues and under allconditions tested.

Potential Applications

Since G1064 transgenic plants have an altered response to the pathogenBotrytis cinerea, G1064 or its equivalogs could be used to manipulatethe defense response in order to generate pathogen-resistant plants.

G1069 (SEQ ID NO: 221)

Published Information

The sequence of G1069 was obtained from EU Arabidopsis sequencingproject, GenBank accession number Z97336, based on its sequencesimilarity within the conserved domain to other AT-Hook related proteinsin Arabidopsis.

Closely Related Genes from Other Species

G1069 protein shares a significant homology to a cDNA isolated fromLotus japonicus nodule library. Similarity between G1069 and the LotuscDNA extends beyond the signature motif of the family to a level thatwould suggest the genes are orthologous. Therefore the gene representedby EST AW720668 may have a function and/or utility similar to that ofG1069.

Experimental Observations

The sequence of G1069 was experimentally determined and the function ofG1069 was analyzed using transgenic plants in which G1069 was expressedunder the control of the 35S promoter.

Plants overexpressing G1069 showed changes in leaf architecture, reducedoverall plant size, and retarded progression through the life cycle.This is a common phenomenon for most transgenic plants in which AT-HOOKproteins are overexpressed if the gene is predominantly expressed inroot in the wild-type background. G1069 was predominantly expressed inroots, based on analysis of RT-PCR results. To minimize thesedetrimental effects, G1069 may be overexpressed under a tissue specificpromoter such as root- or leaf-specific promoter or under induciblepromoter.

One of G1069 overexpressing lines showed more tolerance to osmoticstress when they were germinated in high sucrose plates. This line alsoshowed insensitivity to ABA in a germination assay.

Potential Applications

The osmotic stress results indicate that G1069 could be used to alter aplant's response to water deficit conditions and, therefore, the gene orits equivalogs could be used to engineer plants with enhanced toleranceto drought, salt stress, and freezing.

G1069 affects ABA sensitivity, and thus when transformed into a plantthe gene or its equivalogs may diminish cold, drought, oxidative andother stress sensitivities, and also be used to alter plantarchitecture, and yield.

G1073 (SEQ ID NO: 223)

Published Information

G1073 has been identified in the sequence of a BAC clone from chromosome4 (BAC clone F23E12, gene F23E12.50, GenBank accession number AL022604),released by EU Arabidopsis Sequencing Project.

Closely Related Genes from Other Species

G1073 has similarity to Medicago truncatula cDNA clones (GenBankaccession number AW574000 and AW560824) and Glycine max cDNA clones(AW349284 and A1736668) in the database.

Experimental Observations

The function of G1073 was analyzed using transgenic plants in whichG1073 was expressed under the control of the 35S promoter. Transgenicplants overexpressing G1073 were substantially larger than wild-typecontrols, with at least a 60% increase in biomass. The increased mass of35S::G1073 transgenic plants was attributed to enlargement of multipleorgan types including leaves, stems, roots and floral organs. Petal sizein the 35S::G1073 lines was increased by 40-50% compared to wild typecontrols. Petal epidermal cells in those same lines were approximately25-30% larger than those of the control plants. Furthermore, 15-20% moreepidermal cells per petal were produced compared to wild type. Thus, atleast in petals, the increase in size was associated with an increase incell size as well as in cell number. Additionally, images from the stemcross-sections of 35S::G1073 plants revealed that cortical cells arelarge and that vascular bundles contained more cells in the phloem andxylem relative to wild type

Seed yield was increased compared to control plants. 5S::G1073 linesshowed an increase of at least 70% in seed yield. This increased seedproduction was associated with an increased number of siliques perplant, rather than seeds per silique.

Flowering of G1073 overexpressing plants was delayed. Leaves of G1073overexpressing plants were generally more serrated than those ofwild-type plants. Improved drought tolerance was observed in 35S::G1073transgenic lines.

Potential Applications

Transgenic plants overexpressing G1073 are large and late flowering withserrated leaves. Large size and late flowering produced as a result ofG1073 or equivalog overexpression would be extremely useful in cropswhere the vegetative portion of the plant is the marketable portion(often vegetative growth stops when plants make the transition toflowering). In this case, it would be advantageous to prevent or delayflowering with the use of this gene or its equivalogs in order toincrease yield (biomass). Prevention of flowering by this gene or itsequivalogs would be useful in these same crops in order to prevent thespread of transgenic pollen and/or to prevent seed set. This gene or itsequivalogs could also be used to manipulate leaf shape and droughttolerance.

G1075 (SEQ ID NO: 225)

Published Information

The sequence of G1075 was obtained from the Arabidopsis genomesequencing project, GenBank accession number AC004667, based on itssequence similarity within the conserved domain to other AT-Hook relatedproteins in Arabidopsis.

Closely Related Genes from Other Species

G1075 is homologous to a Medicago truncatula cDNA clone (acc#AW574000

Experimental Observations

The function of G1075 was analyzed using transgenic plants in whichG1075 was expressed under the control of the 35S promoter.Overexpression of G1075 produced very small, sterile plants. Pointedleaves were noted in some seedlings, and twisted or curled leaves andabnormal leaf serrations were noted in rosette stage plants. Bolts wereshort and thin with short internodes. Flowers from severely affectedplants had reduced or absent petals and stamen filaments that partiallyor completely fail to elongate. Because of the severe phenotypes ofthese T1 plants, no T2 seed was produced for physiological andbiochemical analysis.

RT-PCR analysis indicated that G1075 transcripts are found primarily inroots. The expression of G1075 appeared to be induced by cold and heatstresses.

Potential Applications

G1075 or its equivalogs could be used to modify plant architecture anddevelopment, including flower structure. If expressed under aflower-specific promoter, the gene or its equivalogs might also beuseful for engineering male sterility. Because expression of G1075 isroot specific, its promoter could be useful for targeted gene expressionin this tissue.

G1084 (SEQ ID NO: 227)

Published Information

G1084 was discovered as a type 2 bZIP gene in BAC F19F24, accessionnumber AC002392, released by the Arabidopsis genome initiative.

Experimental Observations

The boundaries of G1084 were experimentally determined and the functionof G1084 was analyzed using transgenic plants in which G1084 wasexpressed under the control of the 35S promoter.

Plants overexpressing G1084 showed more disease symptoms followinginoculation with a low dose of the fungal pathogen Botrytis cinerea.G1084 expression appeared to be restricted to flowers and embryos andwas not significantly induced by any conditions tested. Interestingly,one of twenty T1 plants showed heritable alterations in flowerdevelopment. One explanation for this is that this phenotype was causedby silencing of endogenous G1084 in that particular line, causing aphenotype similar to that produced if G1084 was knocked out. No alteredphenotypes were detected in any biochemical assay performed.

Potential Applications

G1084 or its equivalogs could be used to manipulate the plant defenseresponse to produce pathogen-resistant plants.

G1089 (SEQ ID NO: 229)

Published Information

G1089 was initially identified as a gene represented by Arabidopsis ESTH37430. Subsequently, the entire sequence of G1089 was identified in BACF19K6, GenBank accession number AC037424, released by the Arabidopsisgenome initiative.

Closely Related Genes from Other Species

The most related gene to G1089 is a rice gene represented by NCBI entryg13124871. Similarity between G1089 and the rice gene extends beyond thesignature motif of the family to a level that would suggest the genesare orthologous. Therefore the gene represented by the rice gene mayhave a function and/or utility similar to that of G1089

Experimental Observations

The boundaries of G1089 were experimentally determined and the functionof G1089 was analyzed using transgenic plants in which this gene wasexpressed under the control of the 35S promoter. G1089 overexpressingplants had reduced seedling vigor and were characterized as being small,yellow and sickly looking. In addition, a T-DNA knockout of G1089 wasisolated. G1089 knockout mutant plants showed more tolerance to osmoticstress in a germination assay in two separate experiments. They showedmore seedling vigor than wild-type control when germinated on platescontaining high sucrose. G1089 appeared to be constitutively expressed.

Potential Applications

The osmotic stress results indicate that G1089 or its equivalogs couldbe used to alter a plant's response to water deficit conditions and,therefore, may be used to engineer plants with enhanced tolerance todrought, salt stress, and freezing.

G1134 (SEQ ID NO: 231)

Published Information

A partial sequence of G1134 was identified from an EST clone (GenBankaccession number AI099951).

Experimental Observations

A partial sequence of G1134 was identified from an EST clone (GenBankaccession number AI099951). The 5′ end of the G1134 coding sequence wasdetermined by RACE. The function of G1134 was analyzed using transgenicplants in which G1134 was expressed under the control of the 35Spromoter. Primary transformants of G1134 were small with strongly curledleaves. In the T2 generation, two lines had narrow, somewhat curledleaves and siliques with altered shape. A third line segregated forsmall size. Additionally, plants overexpressing G1134 showed an alteredresponse to the growth hormone ethylene. Seeds that were germinated onACC plates in the dark had longer hypocotyls than the correspondingcontrols and occasionally lacked the apical hook that is part of atypical ethylene triple response. In addition, seeds from all linesgerminated in the dark have a partial light grown phenotype in thattheir cotyledons are open and the hypocotyl is straight instead ofcurled.

The results from morphological and physiological analysis indicated thatG1134 protein may play important roles in the regulation of ethylenebiosynthesis, ethylene signal transduction pathways, orphotomorphogenesis. Analysis of G1134 overexpressors revealed noapparent biochemical changes when compared to wild-type control plants.Analysis of the endogenous expression level of G1134, as determined byRT-PCR, revealed that G1134 was predominantly expressed in flowertissues. Expression of G1134 was not induced by any of the environmentalconditions or pathogens tested.

Potential Applications

G1134 or its equivalogs could be used to alter how plants respond toethylene and/or light. For example, it could be used to manipulate fruitripening.

G1140 (SEQ ID NO: 233)

Published Information

G1140 corresponds to gene AT4g24540 (CAB79364), and has also beenreferred to as AGL24 (Alvarez-Buylla et al. (2000a) Proc. Natl. Acad.Sci. USA 97:5328-5333; Alvarez-Buylla et al. (2000b) Plant J. 200024:457-466).

Closely Related Genes from Other Species

G1140 shows sequence similarity outside of the conserved MADS domainwith a variety of MADS proteins from different plant species, such asgi13448660 (MADS box transcription factor from Ipomoea batatas).

Experimental Observations

The function of G1140 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter.

Overexpression of G1140 produced marked alterations in flowermorphology, which were initially observed in a relatively smallproportion ( 6/20 lines) of the T1 plants. Alterations includedvariations in organ size and number. In particular, increased numbers ofpetals and sepals were often present, and sometimes small carpel-likeoutgrowths were fused to the central pair of carpels. Additionally,petals sometimes displayed leaf-like characteristics. It should benoted, however, that these abnormalities were most prevalent in earlyflowers and that later-arising flowers were sometimes normal. Two of thelines selected for subsequent studies showed these floral phenotypes,which became much more extreme in the T2 populations. Some of theeffects resembled those produced by strong apetala1 or apetala2 alleles.Lowermost floral nodes were replaced by shoot-like structures, whichbecame increasingly flower-like towards the top of the inflorescence. Inthe lower structures, floral organs were not apparent and were replacedby bract-like organs that were not organized into whorls. Later‘flowers’ had sepals and petals replaced by bract like organs, butindividual ‘whorls’ could be discerned. Stamens and carpels often hadbract-like characteristics and carpels were frequently unfused.Furthermore, internode elongation was commonly observed between floralwhorls.

G1140 is a MADS box gene of the MIKC-type, and many members of thatsubfamily are involved in the control of flower development.Determination of the functions of MADS box genes has often required thecharacterization of loss-of-function mutants. However, G1140 knockoutmutant plants were wild-type in morphology and development, as well asin the physiological and biochemical analyses that were performed. Inthat mutant line, the T-DNA insertion lies shortly downstream of theconserved MADS domain, within an exon. Within the Arabidopsis MADS-boxgene family, G1140 is most closely related to G861/SHORT VEGETATIVEPHASE, which is involved in the floral transition (Hartmann et al.(2000) Plant J. 21:351-360).

G1140 was expressed in roots, leaves, shoots, and floral tissues. G1140expression was not detected in embryo, siliques, or germinatingseedlings. The expression of the gene did not appear to be significantlyinduced by any of the conditions tested.

Potential Applications

Based on the phenotypes observed in 35S::G1140 plants, the gene or itsequivalogs could be used to manipulate flower structure and development.

G1143 (SEQ ID NO: 235)

Published Information

The sequence of G1143 was obtained from the Arabidopsis genomesequencing project, GenBank accession number AL031187, based on itssequence similarity within the conserved domain to other bHLH/Mycrelated proteins.

Experimental Observations

The function of G1143 was analyzed using transgenic plants in whichG1143 was expressed under the control of the 35S promoter. 35S::G1143transgenic plants showed no consistent differences in morphology towild-type controls. In a first sowing of the T2 populations, it wasobserved that the plants were possibly early flowering. However, thisphenotype was not apparent in either a replant of the T2 lines or in anyT1 plants.

As measured by NIR, G1143 overexpressing plants were found to havedecreased seed oil content and increased seed protein content comparedto wild-type plants.

Potential Applications

G1143 or equivalog overexpression may be used to alter seed oil and seedprotein content in plants, which may be very important for thenutritional value and production of various food products.

G1146 (SEQ ID NO: 237)

Published Information

G1146 corresponds to the ZWILLE and PINHEAD/ZWILLE gene described byMoussain et al. ((1998) EMBO J. 17: 1799-1809) and Lynn et al. ((1999)Development 126: 469-481). Moussain et al. have shown that G1146 isrequired to establish the central-peripheral organization of the embryoapex and that this step is critical for shoot meristemself-perpetuation. They indicate that G1146 is required to maintain stemcells of the developing shoot meristem in an undifferentiated stateduring the transition from embryonic development to repetitivepost-embryonic organ formation. Based upon the results of Moussain et alfrom in situ hybridization analysis, G1146 is found in provascular cellsat all stages of development.

Lynn et al. describe the phenotype of a plant with a mutation on G1146.Early in development, G1146 mutant plants have abnormal embryos, withaberrant division of the upper cells of the suspensor. In youngseedling, there is a radially symmetric pin-like structure in theposition normally occupied by the shoot apical meristem. As developmentproceeds, new shoot meristems eventually arise in the axils of thecotyledons. Phenotypes observed in older plants include trumpet-shapedleaves and abnormalities in the primary inflorescence. Based upon theirresults from northern blot analysis, G1146 expression can be detected inroots, leaves, siliques and inflorescences of developing and matureplants. In the developing embryo, G1146 expression is found in theembryo proper and in the uppermost cell of the suspensor, as determinedby in situ hybridization analysis.

Closely Related Genes from Other Species

The amino acid sequence for a region of the Oryza sativa chromosome 6clone OJ1057_A09 (GenBank accession number AP003986) is significantlyidentical to G1146 outside of the PAZ conserved domains. Therefore, thegene represented by this region of the rice clone may be the ortholog ofG1146.

Experimental Observations

The function of this gene was analyzed using transgenic plants in whichG1146 was expressed under the control of the 35S promoter. Transgenicplants overexpressing G1146 had leaves that had a severe inward curl.The phenotype of these transgenic plants was wild-type in all otherassays performed. G1146 expression was detected in all tissues tested,with expression being highest in flowers, rosette tissue, developingseeds and siliques. Expression of G1146 was not induced by any of theenvironmental or stress conditions tested.

Potential Applications

On the basis of analyses performed to date, G1146 or its equivalogs canbe used in ornamental horticulture to create plants with altered leafmorphology.

G1196 (SEQ ID NO: 239)

Published Information

G1196 was identified by amino acid sequence similarity to ankyrin repeatproteins. G1196 is found in the sequence of the 4, BAC clone T16H5(GenBank AL024486.1 GI:3250673), released by the Arabidopsis GenomeInitiative. The start and stop codons were correctly predicted. Theclosest homologous Arabidopsis protein is NPR1, which is required fordevelopment of systemic acquired resistance in plants (Cao et al. (1997)Cell 88:57-63).

Experimental Observations

RT-PCR analysis of the endogenous level of G1196 transcripts revealedlow constitutive expression in all tissue examined. G1196 transcriptlevels increased upon auxin, ABA, cold, heat and salt treatment, as wellas 7 days post-inoculation with Erysiphe orontii. Plants treated with SAshowed moderate accumulation of G1196 transcripts. The physiologicalanalysis of a G1196 null mutant line revealed increased susceptibilityto a low dose inoculum of Botrytis cinerea. This finding indicated thatG1196 may play a similar role to NPR1 in disease pathways. Apart fromdisease susceptibility, the functional characterization of the G1196null mutant revealed no significant changes in the biochemical profile,the morphology and development, or the response to biotic/abiotic stresstreatments in comparison to the wild-type controls.

Potential Applications

Lack of G1196 activity in a null mutant has been shown to affect theonset of disease following inoculation with Botrytis cinerea. Therefore,G1196 or its equivalogs could be used to manipulate the defense responsein order to generate pathogen-resistant plants.

G1198 (SEQ ID NO: 241)

Published Information

The entire sequence of G1198 was reported in BAC T23G18, accessionnumber AC011438, released by the Arabidopsis genome initiative.

Closely Related Genes from Other Species

G1198 is very similar to the tobacco bZIP transcription factor TGA2.2(accession number AF031487). Similarity extends well beyond theconserved domain, suggesting that G1198 and TGA2.2 have similarfunctions.

Experimental Observations

The boundaries of G1198 were experimentally determined and the functionof G1198 was analyzed using transgenic plants in which this gene wasexpressed under the control of the 35S promoter. G1198 overexpressingplants were reduced in size with smaller, narrower leaves and hadsignificantly increased levels of a glucosinolate as compared to wildtype. G1198 did not appear to be expressed in rosette leaves, but wasexpressed in other tissues.

G1198 overexpressing plants were found to have increased seed oilcontent, as compared to wild-type plants.

Potential Applications

G1198 or equivalog overexpression maybe used to alter seed oil contentin plants, which may be very important for the nutritional value andproduction of various food products.

G1225 (SEQ ID NO: 243)

Published Information

The sequence of G1225 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AB016882, based on its sequencesimilarity within the conserved domain to other bHLH related proteins inArabidopsis.

Experimental Observations

The complete sequence of G1225 was determined. G1225 expression wasdetected in rosette leaves, flowers, embryos and siliques. No expressionwas detected in shoots, roots or germinating seeds. G1225 was notinduced by any condition tested. It may possibly be repressed by coldand Erysiphe infection.

The function of this gene was analyzed using transgenic plants in whichG1225 was expressed under the control of the 35S promoter. G1225overexpressors showed greener cotyledons and longer roots on highsucrose and glucose containing media compared to wild-type controls.This effect was seen in two of the three lines tested. G1225 may thus beinvolved in sugar sensing. Plants overexpressing G1225 were also foundto flower earlier than control plants. 35S::G1225 transformants from twoindependent T2 lines produced visible flower buds several days earlierthan controls, in each of two separate plantings. A similar decrease inflowering time was also seen in thirteen out of twenty T1 lines. Infact, 35S::G1225 seedlings appeared to develop rather more rapidly thanwild type and progressed through the lifecycle at a faster rate.Overexpression of G1225 in Arabidopsis did not result in any biochemicalphenotypic alteration.

The sugar sensing phenotype of G1225 overexpressing plants may berelated to the early flowering phenotype. Sugars are central regulatorymolecules that control several aspects of plant physiology, metabolism,and development, including flowering.

Potential Applications

G1225 or its equivalogs may be useful for accelerating flowering time.

The sugar sensing phenotype of G1225 indicates that this gene or itsequivalogs may be also useful for altering source-sink relationships orother sugar regulated processes.

G1226 (SEQ ID NO: 245) Experimental Observations

The function of this gene was studied using transgenic plants in whichG1226 was expressed under the control of the 35S promoter. Approximately50% of 35S::G1226 primary transformants flowered earlier than wild-typecontrols under continuous light conditions. However, no correlation wasnoted between transgene expression level (determined by RT-PCR, notshown) and this phenotype; some T1 plants that appeared wild-typeclearly expressed the transgene. Marginally early flowering was noted inone of three T2 lines, but the other two lines appeared wild-type.Kanamycin segregation data indicated that all three lines containedsingle locus transgene insertions. RT-PCR analysis indicates that G1226was constitutively expressed in all tissues, with the exception ofroots.

As measured by NIR, G1226 overexpressors had increased seed oil contentcompared to wild-type plants.

Potential Applications

G1226 or equivalog overexpression may be used to alter seed oil content,which may be very important for the nutritional value and production ofvarious food products

G1226 or its equivalogs could be used to manipulate the flowering time.

G1229 (SEQ ID NO: 247) Experimental Observations

RT-PCR analysis indicated that G1229 was expressed in all tissues exceptroots. Its expression level was increased by auxin treatment andrepressed by Erysiphe treatment.

The function of G1229 was studied using transgenic plants in which thisgene was expressed under the control of the 35S promoter. Overexpressionof G1229 strongly influenced plant development. G1229 T1 overexpressingplants were consistently small, paler in color, had rounder leaves, andwere slower growing than wild type. These effects were attenuated tosome extent in the T2 generation, but were still apparent in two of thethree lines analyzed. Physiological assays revealed that G1229overexpressing lines had reduced seed germination and seedling vigorcompared to wild-type plants when grown on MS plates. Plants from G1229overexpressing lines also showed an ethylene sensitive phenotype whengerminated in the dark on media containing ACC. However, becausegermination was generally poor, the interpretation of this phenotype isdifficult.

A single line showed a number of additional phenotypic differences; inthis line flower structure was altered and abnormal seed was producedthat appeared darker and more wrinkled than wild-type seed. Seeds fromthis line showed a significant decrease in oil content as measured byNIR. This observation has been repeated. It is possible that thesemultiple phenotypes could be due to disruption of an endogenous gene bythe transgene insertion, rather than to overexpression of G1229.

Potential Applications

Based on the current analysis of G1229 overexpressing plants, potentialutilities for G1229 or its equivalogs are decrease seed oil contents incrop plants.

G1255 (SEQ ID NO: 249)

Published Information

G1255 was identified as a gene in the sequence of BAC AC079281, releasedby the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G1255 showed strong homology to a putative rice zing finger proteinrepresented by sequence AC087181_(—)3. Sequence identity between thesetwo proteins extends beyond the conserved domain, and therefore, thesegenes can be orthologs.

Experimental Observations

The sequence of G1255 (SEQ ID NO: 249) was experimentally determined andG1255 was analyzed using transgenic plants in which G1255 was expressedunder the control of the 35S promoter. Plants overexpressing G1255 hadalterations in leaf architecture, a reduction in apical dominance, anincrease in seed size, and showed more disease symptoms followinginoculation with a low dose of the fungal pathogen Botrytis cinerea.G1255 was constitutively expressed and not significantly induced by anyconditions tested

Potential Applications

On the basis of the phenotypes produced by overexpression of G1255,G1255 or its equivalogs can be used to manipulate the plant's defenseresponse to produce pathogen resistance, alter plant architecture, oralter seed size.

G1266 (SEQ ID NO: 251)

Published Information

G1266 corresponds to ERF1, ‘ethylene response factor 1’ (GenBankaccession number AF076277) (Solano et al. (1998) Genes Dev. 12:3703-3714). ERF1 was isolated in a search for Arabidopsis EREBP-likegenes using a PCR-based approach. ERF1 expression was shown to berapidly induced by ethylene, and to be dependent on the presence offunctional EIN3 (ETHYLENE-INSENSITIVE3), as no expression was detectedin ein3-1 mutants (Solano et al. (1998) supra). Furthermore, ERF1 mRNAshowed constitutive high-level expression in 35S::EIN3-expressingtransgenic plants, and EIN3 was shown to bind to sequences in the ERF1promoter in a sequence-specific manner (Solano et al. (1998) supra). Allthese results indicated that ERF1 is downstream of EIN3 in the ethylenesignaling pathway, and that both proteins act sequentially in a cascadeof transcriptional regulation initiated by ethylene gas (Solano et al.(1998) supra). ERF1 binds specifically to the GCC element, which is aparticular type of ethylene response element that is found in thepromoters of genes induced upon pathogen attack (Solano et al., (1998)supra). 35S::ERF1-expressing transgenic plants displayed phenotypessimilar to those observed in the constitutive ethylene response mutantctrl or in wild-type plants exposed to ethylene; however, expression ofonly a partial seedling triple response in these lines indicated thatERF1 mediates only a subset of the ethylene responses (Solano et al.(1998) supra). At the adult stage, 35S::ERF1-expressing transgenicplants showed a dwarf phenotype, and some ethylene-inducible genes, likebasic-chitinase and PDF1.2 were constitutively activated in those lines(Solano et al. (1998) supra). All these results showed that ERF1 is adownstream ethylene signaling pathway gene.

Closely Related Genes from Other Species

The sequences of Nicotiana tabacum S25-XP1 (GenBank accession numberAAB38748) and G1266 are very similar, with similarity between the twoproteins extending beyond the conserved AP2 domain.

Experimental Observations

The function of G1266 was further analyzed using transgenic plants inwhich this gene was expressed under the control of the 35S promoter. Asexpected from the previously published work, G1266 overexpressing plantsshowed a dwarf phenotype. In physiological assays, it was shown thatG1266 overexpressing plants were more tolerant to infection with amoderate dose of the fungal pathogen Erysiphe orontii. The resistancephenotype to the fungal pathogen Erysiphe orontii has been repeated.This phenotype might be a consequence of ERF1 being a downstreamethylene signaling pathway gene. Constitutive expression of G1266 mightaccelerate leaf senescence, which in turn might impair infection byErysiphe orontii.

In addition, when analyzed for leaf insoluble sugar composition, threelines showed alterations in rhamnose, arabinose, xylose, and mannose,and galactose when compared with wild-type plants.

Potential Applications

G1266 has been shown to be a downstream ethylene signaling pathway gene,and experiments implicate this gene in the plant response to the fungalpathogen Erysiphe orontii. G1266 or its equivalogs could therefore beused to engineer plants with a modulated response to that and otherpathogens, for example, plants showing increased resistance.

G1275 (SEQ ID NO: 253)

Published Information

G1275 was first identified in the sequence of BAC T19G15 (GenBankaccession number AC005965).

Experimental Observations

The cDNA sequence of G1275 was determined. G1275 was ubiquitouslyexpressed, although expression levels differed among tissues. It ispossible that G1275 expression is induced by several stimuli, includinginfection by Erysiphe, Fusarium, and SA treatment.

The function(s) of G1275 were investigated using both knock-out mutantsand overexpressing plants in which this gene was expressed under thecontrol of the 35S promoter.

Primary transformants of G1275 were small with reduced apical dominance.The inflorescence stems produced by these plants did not elongatenormally. The plants were fertile, but seed yield was reduced becausethe plants were severely dwarfed.

In the knock-out mutant, the T-DNA insertion in G1275 was localized inthe second intron of the gene, which is located within the conservedWRKY-box. Such insertion would result in a null mutation (unless thelarge fragment of exogenous sequence is perfectly spliced out from thetranscribed G1275 pre-mRNA). G1275 knock-out mutant plants wereindistinguishable from wild-type controls in all assays performed.

Potential Applications

G1275 or its equivalogs might be used to alter plant development orarchitecture.

G1305 (SEQ ID NO: 255)

Published Information

G1305 is a member of the (R1)R2R3 subfamily of myb transcriptionfactors. G1305 corresponds to the gene MYB10 (Kranz et al. (1998) PlantJ. 16: 263-276).

Experimental Observations

The function of G1305 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1305 in Arabidopsis resulted in seedlings that were more tolerant toheat in a germination assay. Seedlings from G1305 overexpressingtransgenics were greener than the control seedlings under hightemperature conditions. In a repeat experiment, two lines showed theheat tolerant phenotype. In addition, plants from two of the 35S::G1305T2 lines flowered several days earlier than wild type in each of twoindependent sowings (24 hour light conditions). The plants had ratherflat leaves compared to controls and formed slightly thin inflorescencesin some cases.

According to RT-PCR, G1305 was expressed ubiquitously and expression ofthe gene was unaltered in response to the environmental stress-relatedconditions tested.

Potential Applications

On the basis of the analyses performed to date, the potential utility ofG1305 or its equivalogs is to regulate a plant's time to flower.

G1305 or its equivalogs may also be used to improve heat tolerance atgermination. The germination of many crops is very sensitive totemperature. A gene that would enhance germination in hot conditions maybe useful for crops that are planted late in the season or in hotclimates.

G1322 (SEQ ID NO: 257)

Published Information

G1322 is a member of the (R1)R2R3 subfamily of myb transcriptionfactors. G1322 corresponds to Myb57, a gene identified by Kranz et al.((1998) Plant J. 16: 263-276). The authors used a reverse-Northern blottechnique to study the expression of this gene in a variety of tissuesand under a variety of environmental conditions. They were unable todetect the expression of G1322 in any tissue or treatments tested (Kranzet al. (1998) Plant J. 16: 263-276).

Closely Related Genes from Other Species

G1322 shows sequence similarity with known genes from other plantspecies within the conserved Myb domain.

Experimental Observations

G1322 (SEQ ID NO: 257) was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G1322transgenic plants were wild-type in phenotype with respect to thebiochemical analyses performed. Overexpression of G1322 produced changesin overall plant size and leaf development. At all stages, 35S::G1322plants were distinctly smaller than controls and developed curleddark-green leaves. Following the switch to flowering, the plants formedrelatively thin inflorescence stems and had a rather poor seed yield. Inaddition, overexpression of G1322 resulted in plants with an alteredetiolation response as well as enhanced tolerance to germination underchilling conditions. When germinated in the dark, G1322 overexpressingtransgenic plant lines had open, slightly green cotyledons. Underchilling conditions, all three transgenic lines displayed a similargermination response, seedlings were slightly larger and had longerroots. In addition, an increase in the leaf glucosinolate M39480 wasobserved in all three T2 lines. According to RT-PCR analysis, G1322 wasexpressed primarily in flower tissue.

Potential Applications

The utilities of G1322 or its equivalogs include altering a plant'schilling sensitivity and altering a plant's light response. Thegermination of many crops is very sensitive to cold temperatures. A genethat will enhance germination and seedling vigor in the cold hastremendous utility in allowing seeds to be planted earlier in the seasonwith a higher survival rate.

G1322 or its equivalogs can also be useful for altering leafglucosinolate composition. Increases or decreases in specificglucosinolates or total glucosinolate content are desirable dependingupon the particular application. Modification of glucosinolatecomposition or quantity can therefore afford increased protection frompredators. Furthermore, in edible crops, tissue specific promoters canbe used to ensure that these compounds accumulate specifically intissues, such as the epidermis, which are not taken for consumption.

G1323 (SEQ ID NO: 259)

Published Information

Kranz et al. ((1998) Plant J. 16: 263-276) published a partial cDNAsequence corresponding to G1323, naming it MYB58. Reverse-Northern dataindicates that this gene is expressed primarily in leaf tissue.

Experimental Observations

The complete sequence of G1323 was determined. As determined by RT-PCR,G1323 was highly expressed in embryos, and was expressed atsignificantly lower levels in the other tissues tested. G1323 expressionwas not induced by any stress-related treatments. The function of thisgene was analyzed using transgenic plants in which G1323 was expressedunder the control of the 35S promoter. Primary transformants of G1323were uniformly small and dark green, and a few were late flowering.According to the biochemical analysis of G1323 overexpressors, two hadhigher seed protein. The higher seed protein and lower seed oil contentwas observed in a repeated experiment.

Potential Applications

G1323 or its equivalogs could be used to alter seed protein and oilamounts and/or composition, which is very important for the nutritionalvalue and production of various food products.

G1330 (SEQ ID NO: 261)

Published Information

G1330 is a member of the R2-R3 subfamily of Myb transcription factors.Kranz et al. ((1998) Plant J. 16: 263-276) published a partial cDNAsequence corresponding to G1330, naming it MYB78. Expression of thisgene was not detected by Reverse-Northern analysis in any tissue orunder any environmental treatment tested.

Closely Related Genes from Other Species

G1330 is closely related to a family of novel myb-related genes (Cpm5, 7and 10) from the resurrection plant Craterostigma plantagineum arespecifically expressed in callus and roots in response to ABA ordesiccation (Iturriaga et al. (1996) Plant Mol. Biol. 32: 707-716) aswell as to myb genes from several other crop species. The most relatedgene to G1330 is a tomato gene represented by EST EST276215. Similaritybetween G1330 and the tomato gene extends beyond the signature motif ofthe family to a level that would suggest the genes are orthologous.Therefore the gene represented by EST 276215 and the cpm genes may havea function and/or utility similar to that of G1330.

Experimental Observations

The complete sequence of G1330 was determined. The function of this genewas analyzed using transgenic plants in which G1330 was expressed underthe control of the 35S promoter. Overexpression of G1330 producedchanges in plant growth and development. 35S::G1330 primarytransformants were consistently small with abnormal phyllotaxy, andoften developed spindly inflorescences that yielded few seeds. Theseeffects were also observed in the T2 generation; all three linesappeared markedly small at the seedling stage and often did not survivethe transfer from agar plates to soil. High anthocyanin levels were alsonoted in the T2 (and T3) seedlings of one line. At later stages, T2plants appeared spindly and had very poor seed yield. Kanamycinsegregation data were consistent with 35S::G1330 having deleteriouseffects; all three lines had a deficit of resistant plants, indicatingthat the transgene might be lethal above a certain threshold dosage.

Plants from G1330 overexpressing lines showed an ethylene insensitivephenotype when germinated in the dark on media containing ACC. Seedlingsfrom the three lines tested lacked components of the triple responseincluding the apical hook and to some degree, stunting of the hypocotyl.In addition, plants from the three overexpressing lines had opencotyledons in the dark, which indicated this gene is involved in a lightdependent response,

As determined by RT-PCR, G1330 was highly expressed in roots, and wasexpressed at significantly lower levels in flowers, embryos andseedlings. No expression of G1330 was detected shoots, rosette leaves orsiliques. G1330 expression was repressed in rosette leaves by cold, andosmotic stress treatments and by infection with the phytopathogenErysiphe orontii.

Potential Applications

Because anti-oxidants such as tocopherols and carotenoids are reportedto have anti-cancer and other nutritional properties, G1330 or itsequivalogs could be used to manipulate the nutritional qualities ofplants.

G1330 or its equivalogs could be used to alter how plants respond toethylene. For example, it could be used to manipulate fruit ripening.

G1331 (SEQ ID NO: 263)

Published Information

G1331 is a member of the (R1)R2R3 subfamily of myb transcriptionfactors. G1331 corresponds to Myb79, a gene identified by Kranz et al.((1998) Plant J. 16: 263-276). The authors used a reverse-Northern blottechnique to study the expression of this gene in a variety of tissuesand under a variety of environmental conditions. Kranz et al. wereunable to detect the expression of G1331 in any tissue or treatmentstested (supra).

Closely Related Genes from Other Species

G1331 shows sequence similarity with a protein from alfalfa (BF644787).

Experimental Observations

The function of G1331 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1331 in Arabidopsis did not result in any biochemical phenotypicalteration. However, G1331 overexpression produced highly pleiotropicdevelopmental effects including; changes in leaf morphology, anthocyaninaccumulation, inflorescence abnormalities, and a reduction in overallplant size. In addition, overexpression of G1331 also resulted inseedlings with an altered response to light. In a germination assayconducted in darkness, G1331 seedlings showed opened cotyledons in allthree lines.

G1331 was expressed at low levels in shoots, roots, rosette leaves, andsiliques. G1331 was induced by heat and SA.

Potential Applications

G1331 modifies light response and thus this gene or its equivalogs maybe useful for modifying plant growth or development, for example,photomorphogenesis in poor light, or accelerating flowering time inresponse to various light intensities, quality or duration to which anon-transformed plant would not similarly respond. Elimination ofshading responses may allow increased planting densities with subsequentyield enhancement.

G1332 (SEQ ID NO: 265)

Published Information

G1332 is a member of the (R1)R2R3 subfamily of myb transcriptionfactors. G1332 corresponds to the gene MYB82 (Kranz et al. (1998) PlantJ. 16: 263-276).

Experimental Observations

The function of G1332 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1332 produced a reduction in trichome density on leaf surfaces andinflorescence stems in Arabidopsis. No other phenotypic alterations wereobserved in the G1332 overexpressors.

G1332 was expressed ubiquitously and may have been repressed by Erysipheinfection.

Potential Applications

The potential utility of this gene or its equivalogs is to altertrichome initiation and number in a plant. It would be of greatagronomic value to have plants that produce greater numbers of glandulartrichomes that produce valuable essential oils for the pharmaceuticaland food industries, as well as oils that protect plants against insectand pathogen attack.

G1363 (SEQ ID NO: 267)

Published Information

G1363 was identified based on its similarity to other members of theHap2-like CCAAT-box binding factors. The gene was found in the sequenceof BAC MDC16, GenBank accession number AB019229, released by theArabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G1363 was determined. The function of this genewas then analyzed using transgenic plants in which G1363 was expressedunder the control of the 35S promoter. Transformants weremorphologically indistinguishable from wild-type plants. Biochemicalanalysis of one line indicated the seeds had higher 16:0 in fatty acidcontent. In addition, plants overexpressing G1363 showed fewer diseasesymptoms following infection with the necrotrophic fungal pathogenFusarium oxysporum compared to control plants. The experiment wasrepeated on individual lines, and all three lines showed the phenotype.Wild-type control plants were smaller in the repeat experiment, whichcould affect the disease severity of these plants and accentuate thedegree of tolerance in the overexpressors.

RT-PCR analyses of the endogenous levels of G1363 indicated that thisgene was expressed in all tissues and under all conditions tested.

Potential Applications

Since G1363 activity has been shown to affect the response of transgenicplants to the fungal pathogen Fusarium oxysporum, G1363 or itsequivalogs could be used to manipulate the defense response in order togenerate pathogen-resistant plants.

G1411 (SEQ ID NO: 269)

Published Information

G1411 was identified in the sequence of TAC clone K22G18 (GenBankaccession number AB022212).

Experimental Observations

The complete sequence of G1411 was determined. The function of G1411 wasanalyzed using transgenic plants in which this gene was expressed underthe control of the 35S promoter. G1411 overexpressing plants weresmaller than wild-type controls and showed reduced apical dominance:axillary shoots develop prematurely amongst primary rosette leaves,resulting in a bushy plant. G1411 overexpressing plants behaved like thecorresponding wild-type controls in all physiological and biochemicalassays that were performed.

Potential Applications

G1411 or its equivalogs could be used to manipulate plant architecture.

G1417 (SEQ ID NO: 271)

Published Information

G1417 corresponds to gene AT4g01720 (CAB77742).

Closely Related Genes from Other Species

G1417 shows sequence similarity, outside of the conserved WRKY domain,with a rice protein (gi8467950).

Experimental Observations

The function of G1417 was studied using a line homozygous for a T-DNAinsertion in the gene. The T-DNA insertion lies immediately upstream ofthe conserved WRKY domain coding sequence, and was expected to result ina null mutation. G1417 knockout mutant plants showed reduced seedlingvigor during germination. The G1417 knockout showed alterations in seedfatty acid composition. An increase in 18:2 fatty acid and a decrease in18:3 fatty acid were observed in two seed batches.

G1417 was ubiquitously expressed and did not appear to be significantlyinduced by any of the conditions tested.

Potential Applications

G1417 or its equivalogs could be useful to manipulate the saturationlevels of lipids in seeds. Alteration in seed lipid saturation could beused to improve the heat stability of oils or to improve the nutritionalquality of seed oil.

G1419 (SEQ ID NO: 273)

Published Information

G1419 was identified in the sequence of P1 clone MWD22; it correspondsto gene MWD22.13 (GenBank PID BAA97381).

Closely Related Genes from Other Species

G1419 is most closely related to some non-Arabidopsis AP2/EREBP proteinsthat have been suggested to be involved in the ethylene response, liketobacco EREBP-4.

Experimental Observations

To investigate the function(s) of G1419, this gene was expressed underthe control of the 35S promoter in transgenic plants. G1419overexpressing plants were essentially indistinguishable from wild-typecontrols in all assays performed. Two T2 lines showed alteredbiochemical phenotypes that were different in each one of them: One linehad higher 16:0 when assayed for seed frames, and another line hadhigher seed protein.

G1419 appeared to be ubiquitously expressed.

Potential Applications

G1419 or its equivalogs could be used to increase seed protein, which isvery important for the nutritional value and production of various foodproducts.

G1449 (SEQ ID NO: 275)

Published Information

G1449 is annotated in the sequence of genomic clone MKP6, GenBankaccession number AB022219, released by the Arabidopsis GenomeInitiative.

Experimental Observations

A cDNA clone corresponding to G1449 was isolated from an embryo cDNAlibrary. It was later identified in the sequence of genomic clone MKP6,GenBank accession number AB022219, released by the Arabidopsis GenomeInitiative.

G1449 was expressed at high levels in embryos and siliques, and atsignificantly lower levels in roots and seedlings. It was induced byauxin in leaf tissue. Plants overexpressing G1449 showed floralabnormalities. Primary transformants showed changes in floral organnumber and identity. Large petals were noted in one plant. Affectedlines were also somewhat smaller than controls. These plants producedlittle seed and it was necessary to bulk seed for analysis. One T3 lineproduced flowers that were somewhat larger than control flowers withpetals that were more open. These flowers often had extra petals. G1449mutant plants did not show any other phenotypic alterations in any ofthe physiological or biochemical assays performed.

Potential Applications

Because larger and more open petals are produced in some G1449overexpressing plants, G1449 or its equivalogs may be useful formodifying flower form and size in ornamental plants. The promoter ofG1449 may also be useful to drive gene expression in seeds and seed podsor fruits.

G1451 (SEQ ID NO: 277)

Published Information

G1451 is ARF8, a member of the ARF class of proteins with a VP1-likeN-terminal domain and a C-terminal domain with homology to Aux/IAAproteins. ARF8, like several other ARFs, contains a glutamine-richcentral domain that can function as a transcriptional activation domain(1). ARF8 was shown to bind to an auxin response element (2). It wasalso shown that a truncated version of ARF8 lacking the DNA bindingdomain but containing the activation domain and the C-terminal domaincould activate transcription on an auxin responsive promoter, presumablythrough interactions with another factor bound to the auxin responseelement (1). ARF8 is closely related in sequence to ARF6 (2).

Experimental Observations

G1451 was expressed throughout the plant, with the highest expression inflowers. Transcripts of G1451 were induced in leaves by a variety ofstress conditions. A line homozygous for a T-DNA insertion in G1451 wasused to determine the function of this gene. The T-DNA insertion ofG1451 is approximately one-fifth of the way into the coding sequence ofthe gene and therefore is likely to result in a null mutation.

As measured by NIR, G1451 knockout mutants had increased total combinedseed oil and seed protein content compared to wild-type plants.

Potential Applications

G1451 or its equivalogs may be used to alter seed oil and proteincontent, which may be very important for the nutritional value andproduction of various food products

G1451 or its equivalogs could also be used to increase plant biomass.Large size is useful in crops where the vegetative portion of the plantis the marketable portion since vegetative growth often stops whenplants make the transition to flowering.

G1452 (SEQ ID NO: 279)

Published Information

G1452 was identified in the sequence of clones T22O13, F12K2 withaccession number AC006233 released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G1452 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1452 produced changes in leaf development and markedly delayed theonset of flowering. 35S::G1452 plants produced dark green, flat, roundedleaves, and typically formed flower buds between 2 and 14 days laterthan controls. Additionally, some of the transformants were noted tohave rather low trichome density on leaves and stems. At later stages oflife cycle, 35S::G1452 appeared to develop slowly and senescedconsiderably later than wild-type controls. In addition, G1452overexpressors were more tolerant to osmotic stress, and wereinsensitive to ABA in separate germination assays.

G1452 expression was not detected in any tissue tested by RT-PCR and wasnot induced by any environmental stress-related condition tested.

Potential Applications

On the basis of the analyses performed to date, G1452 or its equivalogscould be use to alter plant growth and development.

In addition, G1452 or its equivalogs could be used to alter a plant'sresponse to water deficit conditions and therefore, could be used toengineer plants with enhanced tolerance to drought and salt stress.

G1463 (SEQ ID NO: 281)

Published Information

G1463 was identified in the sequence of BAC T13D8 with accession numberAC004473 released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G1463 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. In later stageplants, overexpression of G1463 resulted in premature senescence ofrosette leaves. Under continuous light conditions, the most severelyaffected plants started to senesce approximately 10 days earlier thanwild-type controls, at around 30 days after sowing. Additionally,35S::G1463 plants formed slightly thin inflorescence stems and showed arelatively low seed yield. However, it is possible that such featuresdirectly resulted from the loss of photosynthetic capacity caused bypremature senescence. 35S::G1463 transgenic plants were wild-type inphenotype with respect to the physiological and biochemical analysesperformed.

G1463 expression could not be detected in any tissue or in response toenvironmental stress-related conditions tested using RT-PCR.

Potential Applications

On the basis of the analyses performed to date, the potential utilitiesof G1463 or its equivalogs could be used to manipulate senescence inplant tissues. Although leaf senescence is thought to be an evolutionaryadaptation to recycle nutrients, the ability to control senescence in anagricultural setting has significant value. For example, a delay in leafsenescence in some maize hybrids is associated with a significantincrease in yields and a delay of a few days in the senescence ofsoybean plants can have a large impact on yield. Delayed flowersenescence may also generate plants that retain their blossoms longerand this may be of potential interest to the ornamental horticultureindustry.

G1471 (SEQ ID NO: 283)

Published Information

G1471 was identified in the sequence of P1 clone MDK4, GenBank accessionnumber AB010695, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of this gene was analyzed using transgenic plants in whichG1471 was expressed under the control of the 35S promoter. All35S::G1471 primary transformants were markedly small, had narrow curledleaves and formed thin inflorescence stems. Flowers from many T1 plantswere extremely poorly developed, and often had organs missing, reducedin size, or highly contorted. Due to such defects, the fertility wasvery low, and approximately one third of the lines were tiny andcompletely sterile. Plants from one T2 generation line displayedwild-type morphology, indicating that the transgene might have becomesilenced. Two lines, however, were small, had narrow curled leaves andflowered marginally earlier than controls. The phenotype of thesetransgenic plants was wild-type in all other assays performed. G1471appeared to be expressed at medium levels in siliques and embryos.

G1471 overexpressing plants were found to have increased seed oilcontent compared to wild-type plants.

Potential Applications

G1471 or equivalog overexpression may be used to increase seed oilcontent in plants.

Because expression of G1471 is embryo and silique specific, its promotercould be useful for targeted gene expression in these tissues.

G1478 (SEQ ID NO: 285)

Published Information

G1478 was identified as a gene in the sequence of BAC Z97338, releasedby the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G1478 shows some homology to non-Arabidopsis proteins within theconserved domain.

Experimental Observations

The sequence of G1478 (SEQ ID NO: 285) was determined and G1478 wasanalyzed using transgenic plants in which G1478 was expressed under thecontrol of the 35S promoter. Plants overexpressing G1478 had a generaldelay in progression through the life cycle, in particular a delay inflowering time. Plants overexpressing G1478 also showed a increase inseed oil and an decrease in seed protein.

G1478 was expressed at higher levels in flowers, rosettes and embryosbut otherwise expression was constitutive.

Potential Applications

G1478 or its equivalogs can be used to manipulate the rate at whichplants grow, and flowering time.

G1478 can also be used to manipulate seed oil and protein, which can bevery important from a nutritional standpoint.

G1482 (SEQ ID NO: 287)

Published Information

G1482 was identified as a gene in the sequence of BAC AC006434, releasedby the Arabidopsis Genome Initiative.

Experimental Observations

The sequence of G1482 was experimentally determined. The data presentedfor this gene are from plants homozygous for a T-DNA insertion in G1482.The T-DNA insertion of G1482 is in coding sequence and therefore thisknockout mutant is likely to contain a null allele. Homozygous plantsharboring a T-DNA insertion in G1482 displayed significantly more rootgrowth on MS control plates as well as on different stresses in threeseparate experiments. G1482 was constitutively expressed andsignificantly induced by auxin, ABA and osmotic stress.

The function of G1482 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Plantsoverexpressing G1482 contained high levels of anthocyanins.

Potential Applications

Based on the phenotypes produced when this gene is knocked out, G1482 orits equivalogs could be used to manipulate root growth, particularly inresponse to environmental stresses such as drought and low nutrients.

G1482 or its equivalogs could also be used to alter anthocyaninproduction. The potential utilities of this gene includes alterations inpigment production for horticultural purposes, and possibly increasingstress resistance in combination with another transcription factor.Flavonoids have antimicrobial activity and could be used to engineerpathogen resistance. Several flavonoid compounds have health promotingeffects such as the inhibition of tumor growth and cancer, prevention ofbone loss and the prevention of the oxidation of lipids. Increasinglevels of condensed tannins, whose biosynthetic pathway is shared withanthocyanin biosynthesis, in forage legumes is an important agronomictrait because they prevent pasture bloat by collapsing protein foamswithin the rumen. For a review on the utilities of flavonoids and theirderivatives, see Dixon et al. (1999) Trends Plant Sci. 10: 394-400.

G1488 (SEQ ID NO: 289)

Published Information

G1488 was identified as a gene in the sequence of BAC F18A17 (AccessionNumber AC005405), released by the Cold Spring Harbor Laboratory.

Experimental Observations

The function of G1488 was analyzed using transgenic plants in whichG1488 was expressed under the control of the 35S promoter. Plants fromtwo of the three 35S::G1488 T2 populations were rather small at earlystages, formed slightly rounded leaves, and produced thin bushyinflorescence stems that were shorter than those of controls. Thisphenotype was verified when the populations were re-grown. However, inthe second sowing, plants from both lines also flowered early.Overexpression of G1488 in Arabidopsis also resulted in seedlings withan altered response to light. In a germination assay conducted indarkness, G1488 seedlings showed opened cotyledons in all three lines.

G1488 was expressed in all tissues, although it was expressed at higherlevels in embryonic tissue and siliques. G1488 was slightly induced inresponse to ABA treatment or heat stress.

G1488 overexpressors were found to have increased seed protein contentcompared to wild-type plants.

Potential Applications

G1488 modified light response and thus it or its equivalogs may beuseful for modifying plant growth or development, for example,photomorphogenesis in poor light, or accelerating flowering time inresponse to various light intensities, quality or duration to which anon-transformed plant would not similarly respond. Elimination ofshading responses may allow increased planting densities with subsequentyield enhancement.

G1488 or its equivalogs could also be used to manipulate plantarchitecture.

G1488 or its equivalogs might be used to engineer crops with earlierflowering times. Most modern crop varieties are the result of extensivebreeding programs. Many generations of backcrossing may be required tointroduce desired traits. Systems that accelerate flowering could havevaluable applications in such programs since they allow much fastergeneration times. Additionally, in some instances, a faster generationtime might allow additional harvests of a crop to be made within a givengrowing season.

G1488 or equivalog overexpression may be used to alter seed proteincontent in plants

G1494 (SEQ ID NO: 291)

Published Information

The sequence of G1494 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AC006224, based on its sequencesimilarity within the conserved domain to other bHLH related proteins inArabidopsis.

Experimental Observations

The complete sequence of G1494 was determined. G1494 was expressed inall tissues tested except in roots.

The function of this gene was analyzed using transgenic plants in whichG1494 was expressed under the control of the 35S promoter.Overexpression of G1494 produced pleiotropic effects similar to thosecaused by shade avoidance responses or deficiencies in light regulateddevelopment. In particular, the 35S::G1494 phenotype was very similar tothat described for plants mutant in multiple different phytochrome genes(Devlin et al. (1999) Plant Physiol. 119: 909-916), indicating thatG1494 might have a role regulating or responding to light perception.Following germination, 35S::G1494 seedlings formed very long hypocotylsand displayed elongated cotyledon petioles. Rosette leaves weregenerally very pale, narrow, upward pointing, and had long petioles.Such effects were observed in either 12-hour or 24-hour photoperiodicconditions, and in both cases, the plants switched to flowering muchearlier than wild-type controls. In 24-hour light, 35S::G1494 plantsformed flower buds after making 2-4 leaves (wild type typically made12-14 leaves), whereas in 12-hour conditions 4-7 leaves were formed(wild type typically made 25-30 leaves). In addition to this, in35S::G1494 plants, internodes between rosette leaves extended, making adefined rosette difficult to discern. It should be noted that theinflorescences produced by these plants were uniformly extremely thinand spindly and generated very few siliques. Additionally, the seedsfrom one of these T2 populations were consistently large and palecompared to controls.

The morphological alterations in the 35S::G1494 plants were somewhatsimilar to those in the 35S::G2144 plants.

Alterations in leaf prenyl composition were consistently detected in thethree 35S::G1494 lines analyzed, which could be predicted because of themorphological phenotype of 35S::G1494 overexpressors.

Potential Applications

G1494 or its equivalogs could be used to alter how plants respond tolight. For example, it could be used to manipulate plant appearance,growth and development, and flowering time.

G1496 (SEQ ID NO: 293)

Published Information

The genomic sequence of G1496 has been determined as part of theArabidopsis Genome Initiative (BAC clone T30D6, GenBank accession numberAC006439).

Experimental Observations

As determined by RT-PCR, G1496 was highly expressed in rosette leavesand germinating seeds. Expression of G1496 was not induced by anystress-related treatment tested. The function of G1496 was analyzedusing transgenic plants in which G1496 was expressed under the controlof the 35S promoter.

Arabidopsis plants overexpressing G1496 produce more seed oil thanwild-type plants.

Potential Applications

Based on the current analysis of G1496 or equivalog overexpressingplants, potential utilities for G1496 are to increase oil contents incrop plants.

G1499 (SEQ ID NO: 295)

Published Information

The sequence of G1499 was obtained from the Arabidopsis genomesequencing project, GenBank accession number AB020752, based on itssequence similarity within the conserved domain to other bHLH relatedproteins in Arabidopsis.

Closely Related Genes from Other Species

The similarity between G1499 and Brassica rapa subsp. pekinensis flowerbud cDNA (acc#AT002234) is significant not only in the conserved bHLHdomains but also outside of the domains.

Experimental Observations

The function of G1499 was analyzed using transgenic plants in whichG1499 was expressed under the control of the 35S promoter. A range ofphenotypes was observed in primary transformants of G1499. The mostseverely affected plants were smaller than controls, dark green, withstrongly curled leaves, and produced bolts that terminated without aninflorescence. In some cases, flowers were replaced with filamentousstructures or carpelloid structures. Less severely affected linesproduced flowers where sepals were converted to carpelloid tissue.Petals and stamens were absent or reduced in size and number. Mildlyaffected T1 plants that were small in size but produced normal flowerswere taken to the T2 generation. Three T2 lines produced plants thatwere smaller than controls, darker green, and had narrower leaves.

G1499 overexpressors were similar to their wild-type counterparts in allphysiological and biochemical assays.

G1499 was predominantly expressed in the reproductive tissues such asflower, embryo and silique. Lower levels of expression were alsodetected in roots and germinating seeds. It's expression level wasunaffected by any of the environmental conditions tested.

Phenotypes produced by overexpressing G1499 and G779 were similar in theaspects of flower structures. Cluster analysis using basichelix-loop-helix motif revealed that both proteins of G1499 and G779 areclosely related.

Potential Applications

G1499 or its equivalogs could be used to modify plant architecture anddevelopment, including flower structure. If expressed under aflower-specific promoter, it might also be useful for engineering malesterility. Because expression of G1499 is flower and embryo specific,its promoter could be useful for targeted gene expression in thesetissues.

Potential utilities of this gene or its equivalogs also includeincreasing chlorophyll content, allowing more growth and productivity inconditions of low light. With a potentially higher photosynthetic rate,fruits could have higher sugar content. Increased carotenoid contentcould be used as a nutraceutical to produce foods with greaterantioxidant capability.

G1519 (SEQ ID NO: 297)

Published Information

G1519 corresponds to PEX10, which encodes a peroxisome assembly protein(Schumann et al. (1999) Plant Physiol. 119: 1147.

Closely Related Genes from Other Species

G1519 has a homolog in tomatoes (Accession # BE436498).

Experimental Observations

The function of G1519 was analyzed by knockout analysis. Plantsheterozygous for a knockout mutation in G1519 segregate 3 viable:1inviable seeds in the silique. Homozygous G1519 knockout plants couldnot be obtained, due to the embryo lethality of the mutation, so nophysiology or biochemistry assays could be done. G1519 is an essentialgene that is necessary for embryo development.

Potential Applications

Because a knockout mutation in G1519 results in embryo lethality, thegene or its equivalogs are potentially useful as herbicide targets.

G1526 (SEQ ID NO: 299)

Published Information

The transcription regulator G1526 was identified by amino acid sequencesimilarity to proteins of the SWI/SNF family of chromatin remodelingfactors. G1526 is found in the sequence of the chromosome 5 P1 cloneMDJ22 (GenBank AB006699.1 GI:2351064), released by the ArabidopsisGenome Initiative. The translational stop codon was incorrectlypredicted.

Experimental Observations

RT-PCR analysis of the endogenous level of G1526 transcripts revealsthat G1526 was expressed constitutively in all Arabidopsis tissues,except in germinating seeds where no G1526 is detectable. The G1526 nullmutant had higher seed oil content.

Potential Applications

G1526 or its equivalogs may be used to increase seed oil in plant seed,which might be used to increase seed oil yield, and increase the caloriccontent of food for humans and animal feeds.

G1540 (SEQ ID NO: 301)

Published Information

G1540 is the Arabidopsis WUSCHEL (WUS) gene and encodes a novel subclassof homeodomain protein (Mayer et al. (1998) Cell 95:805-815).

WUS is a key developmental protein that has a core role in regulatingthe fate of stem cells within Arabidopsis apical meristems. The centralzone of an apical meristem contains a pool of undifferentiatedpluripotent stems cells. These stem cells are able to both maintainthemselves and supply cells for incorporation into new organs on theperiphery of the meristem (shoot meristems initiate leaves whereasflower meristems initiate whorls of floral organs).

Defects are visible in the shoots and flowers of wus mutants (Laux etal. (1996) Development 122: 87-96; Endrizzi et al. (1996) Plant J.10:967-979). Wus mutants fail to properly organize a shoot meristem inthe developing embryo. Postembryonically, wus shoot meristems becomeflattened and terminate growth prematurely. Leaf primordia and secondaryshoots often initiate ectopically across the surface of these terminatedstructures. The leaf primordia usually develop into a disorganized bunchand a secondary shoot meristem takes over growth. This secondarymeristem then terminates and the developmental pattern is repeated,leading to a plant with no clear main axis of growth and clusters ofleaves at the tips of shoots. Wus floral meristems exhibit a comparablephenotype to the shoot meristem; development often ceases prematurelysuch that flowers either lack the innermost whorls of organs, or possessa single stamen in place of the inner whorls.

The mutant phenotype indicates that wus is required to maintain theidentity of the central zone within apical meristems and prevent thosecells from becoming differentiated. In situ expression patterns of WUSRNA support such a conclusion; WUS is first observed in the embryonicshoot meristem at the 16-cell stage. Later, expression becomes confinedto small groups of cells (in shoot and floral meristems) at the base ofthe central zone where it specifies the fate of overlying cells as stemcells. WUS is thought to be expressed, and act, independently of anotherhomeobox gene, SHOOT MERISTEMLESS (STM), G431, which has a relatedfunction (Long et al. (1996) Development 125:3027-3035). STM isinitially required for the establishment of the shoot meristem duringembryogenesis. Later S™ is expressed throughout the whole meristem domewhere, together with an antagonist, CLAVATA1, it regulates transition ofcells from the central zone towards differentiation and organ formationat the meristem periphery (Clarke et al. (1996) Development 122:1565-1575; Endrizzi et al. (1996) Plant J. 10:967-979). A currenthypothesis is that WUS specifies the identity of central stem cellswhereas STM allows the progeny of those cells to proliferate beforebeing partitioned into organ primordia (Mayer et al. (1998) Cell95:805-815).

The effects of WUS over-expression have not yet been published. However,based on the present model for WUS function, its ectopic expressionmight be expected to induce formation of ectopic meristematic stemcells.

Experimental Observations

Over-expressers for G1540 (WUSCHEL) formed callus-like structures onleaves, stems and floral organs. These observations correlate with theproposed role of WUS in specifying stem cell fate in meristems. In T1over-expressers, cells took on characteristics of stem cells atinappropriate locations, indicating that WUS was sufficient to specifystem cell identity.

Potential Applications

The over-expression phenotype indicates that G1540 is sufficient toconfer stem cell identity on plant cells, and thereby prevent them fromdifferentiating. The gene or its equivalogs might be of utility in themaintenance of plant cell lines grown in vitro, where thedifferentiation of those lines creates difficulties. The gene or itsequivalogs might also be applied to transformation systems forrecalcitrant species, where generation of callus is currentlyproblematic but is required as part of the transformation procedure.

G1543 (SEQ ID NO: 303)

Published Information

G1543 was identified as a novel homeobox gene within section 3 of 255from the complete sequence of Chromosome II (GenBank accession numberAC005560, released by the Arabidopsis Genome Initiative).

Closely Related Genes from Other Species

The G1543 protein is related to a number of HD-ZIP proteins from otherspecies, including OSHOX3 (AAD37696) from rice, with which sequenceidentity extends beyond the conserved homeodomain.

Experimental Observations

The ends of G1543 were determined by RACE and a full-length cDNA wasisolated by PCR from mixed cDNA. The encoded 275 amino acid product wasfound to be a member the HD-ZIP class II group of HD proteins. Thepublic annotation for this gene was incorrect; the protein predicted inthe BAC report was only 162 amino acids in length.

RT-PCR analysis revealed that G1543 was expressed ubiquitously but wasup-regulated in response to auxin applications.

The function of G1543 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G1543Arabidopsis plants exhibited a range of phenotypes; most consistently,however, the plants possessed dark green leaves and an altered branchingpattern that led to a shorter more compact stature. These morphologicalphenotypes, along with the expression data, implicate G1543 as acomponent of a growth or developmental response to auxin.

Biochemical assays reflected the changes in leaf color noted duringmorphological analysis. All three T2 lines examined displayed increasedlevels of leaf chlorophylls and carotenoids. Additionally, one of threelines had a decrease in seed oil combined with an increase in seedprotein. A repeat experiment verified the altered seed oil and proteincomposition in two lines.

Physiological assays identified no clear differences between 35S::G1543and wild-type plants.

Potential Applications

The altered levels of chlorophylls, carotenoids, seed oils, and proteinsthat resulted from overexpression of the gene in Arabidopsis indicatethat G1543 or its equivalogs or its equivalogs might used to manipulatethe composition of these substances in seed, with applications towardthe improvement in the nutritional value of foodstuffs (for example, byincreasing lutein).

Enhanced chlorophyll and carotenoid levels could also improve yield incrop plants. For instance lutein, like other xanthophylls such aszeaxanthin and violaxanthin, is an essential component in the protectionof the plant against the damaging effects of excessive light.Specifically, lutein contributes, directly or indirectly, to the rapidrise of non-photochemical quenching in plants exposed to high light.Crop plants engineered to contain higher levels of lutein couldtherefore have improved photo-protection, possibly leading to lessoxidative damage and better growth under high light. Additionally,elevated chlorophyll levels might increase photosynthetic capacity.

G1543 or its equivalogs might be applied to modify plant stature. Thiscould be used to produce crops that are more resistant to damage by windand rain, or more amenable to harvest. Plants with altered stature mightalso be of interest to the ornamental plant market.

This gene or its equivalogs may also be used to alter oil production inseeds, which may be very important for the nutritional quality andcaloric content of foods

G1634 (SEQ ID NO: 305)

Published Information

G1634 was identified in the sequence of BAC MJJ3, GenBank accessionnumber AB005237, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G1634 was determined. cDNA microarray analysesof the endogenous levels of G1634 indicated that this gene was primarilyexpressed in root and silique tissues. In addition, G1634 expression wasnot altered significantly in response to any of the stress-relatedtreatments tested. The function of this gene was analyzed usingtransgenic plants in which G1634 was expressed under the control of the35S promoter. The phenotype of these transgenic plants was wild-type inall assays performed.

G1634 overexpressors were found to have altered seed protein contentcompared to wild-type plants.

Potential Applications

G1634 or its equivalogs could be used to alter seed protein amountswhich is very important for the nutritional value and production ofvarious food products.

G1637 (SEQ ID NO: 307)

Published Information

G1637 is a member of the myb-related subfamily of Myb transcriptionfactors. G1637 was identified in BAC clone K11J9, accession numberAB012239, release by the Arabidopsis sequencing project.

Closely Related Genes from Other Species

The most related gene to G1637 is a soybean gene represented by ESTAW760127.

Experimental Observations

The complete sequence of G1637 was determined. The function of this genewas analyzed using transgenic plants in which G1637 was expressed underthe control of the 35S promoter. The phenotype of these transgenicplants was wild-type in all assays performed.

RT-PCR analysis of the endogenous levels of G1637 indicated that thisgene was expressed in all tissues and was induced by ABA, drought, anddisease-related treatments.

G1637 overexpressors had increased seed oil and decreased seed proteincontent compared to wild-type plants.

Potential Applications

G1637 or equivalog overexpression may be used to alter seed proteincontent, which may be very important for the nutritional value andproduction of various food products

G1640 (SEQ ID NO: 309)

Published Information

G1640 was identified in the sequence of BAC K21P3, GenBank accessionnumber AB016872, released by the Arabidopsis Genome Initiative.

Experimental Observations

The annotation of G1640 in BAC AB016872 was experimentally confirmed.The function of this gene was then analyzed using transgenic plants inwhich G1640 was expressed under the control of the 35S promoter. Thetransgenic plants were morphologically indistinguishable from wild-typeplants. They were wild-type in all physiological assays performed.Biochemical analysis indicated that overexpression of G1640 inArabidopsis results in an increase in seed oil content and a decrease inseed protein content.

As determined by RT-PCR, G1640 was expressed in leaves, flowers, embryosand siliques. No expression of G1640 was detected in the other tissuestested, nor was the gene induced in rosette leaves by any stress-relatedtreatment.

Potential Applications

G1640 or its equivalogs could be used to decrease seed protein andincrease seed oil amounts and/or composition which is very important forthe nutritional value, caloric content and production of various foodproducts.

G1645 (SEQ ID NO: 311)

Published Information

G1645 is a member of the (R1)R2R3 subfamily of MYB transcriptionfactors. G1645 was identified in the sequence of BAC T24P13, GenBankaccession number AC006535, released by the Arabidopsis GenomeInitiative.

Closely Related Genes from Other Species

G1645 shows extensive sequence similarity to MYB proteins from otherplant species including tomato (AW624217), and alfalfa (AQ917084).

Experimental Observations

The function of G1645 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1645 produced marked changes in Arabidopsis leaf, flower and shootdevelopment. These effects were observed, to varying extents, in themajority of 35S::G1645 primary transformants.

At early stages, many 35S::G1645 T1 lines appeared slightly small andmost had rather rounded leaves. However, later, as the leaves expanded,in many cases they became misshapen and highly contorted. Furthermore,some of the lines grew slowly and bolted markedly later than controlplants. Following the switch to flowering, 35S::G1645 inflorescencesoften showed aberrant growth patterns, and had a reduction in apicaldominance. Additionally, the flowers were frequently abnormal and hadorgans missing, reduced in size, or contorted. Pollen production alsoappeared poor in some instances. Due to these deficiencies, thefertility of many of the 35S::G1645 lines was low and only small numbersof seeds were produced.

Overexpression of G1645 resulted in a low germination efficiency whengerminated on the 32 C heat stress.

As determined by RT-PCR, G1645 was expressed in flowers, embryos,germinating seeds and siliques. No expression of G1645 was detected inthe other tissues tested. G1645 expression appeared to be repressed inrosette leaves infected with the phytopathogen Erysiphe orontii.

Potential Applications

G1645 or its equivalogs could be used to alter inflorescence structure,which may have value in production of novel ornamental plants.

G1645 or equivalog activity could be used to alter a plant's response toheat stress.

G1646 (SEQ ID NO: 313)

Published Information

G1646 was identified in the BAC sequence with GenBank accession numberAB007649, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G1646 was determined. The function of this genewas analyzed using transgenic plants in which G1646 was expressed underthe control of the 35S promoter. G1646 was constitutively expressed atmedium levels in all tissues and environmental conditions tested.

As measured by NIR, G1646 overexpressors had altered seed oil contentcompared to wild-type plants.

Potential Applications

G1646 overexpression may be used to alter seed oil content, which may bevery important for the nutritional value and production of various foodproducts

G1652 (SEQ ID NO: 315)

Published Information

The sequence of G1652 was obtained from the Arabidopsis genomicsequencing project, GenBank accession number AC005617, based on itssequence similarity within the conserved domain to other bHLH relatedproteins in Arabidopsis.

Experimental Observations

The complete sequence of G1652 was determined. No expression of G1652was detected in any of the untreated tissues tested. G1652 may beinduced by cold treatment and Fusarium infection.

The function of this gene was analyzed using transgenic plants in whichG1652 was expressed under the control of the 35S promoter. 35S::G1652transformants were distinctly smaller and slower developing thanwild-type controls, and formed rounded dark-green leaves, and short,thin, inflorescence stems. This phenotype was apparent in the majorityof primary transformants and two of the three T2 lines. Small size wasalso noted in the physiological assays.

G1652 overexpressors had increased seed protein content compared towild-type plants.

Potential Applications

G1652 or equivalog overexpression may be used to alter seed proteincontent, which may be very important for the nutritional value andproduction of various food products

G1652 or its equivalogs may also be useful to regulate some aspect ofplant growth and development.

G1672 (SEQ ID NO: 317)

Published Information

G1672 was first identified in the sequence of the P1 clone MIK19,GenBank accession number AB013392, released by the Arabidopsis GenomeInitiative.

Closely Related Genes from Other Species

The most related gene to G1672 is a rice gene P0710E05.22 in accessionBAA99435.

Experimental Observations

The full length sequence of G1672 was experimentally confirmed. Thefunction of G1672 was analyzed using transgenic plants in which G1672was expressed under the control of the 35S promoter.

RT-PCR analysis was used to determine the endogenous levels of G1672 ina variety of tissues and under a variety of environmental stress-relatedconditions. G1672 was primarily expressed at low levels in shoots,roots, flowers, embryos and siliques. No expression was detected inrosette leaves and germinated seedlings. G1672 did not show anyinduction under any of the different environmental conditions tested.

As measured by NIR, G1672 overexpressors had altered seed oil contentcompared to wild-type plants.

Potential Applications

G1672 or equivalog overexpression may be used to alter seed oil content,which may be very important for the nutritional value and production ofvarious food products

G1677 (SEQ ID NO: 319)

Published Information

G1677 was identified in the sequence of P1 clone:MKM21, GenBankaccession number AB016876, released by the Arabidopsis GenomeInitiative.

Closely Related Genes from Other Species

G1677 shows extensive sequence similarity to a protein from rice(AP004114).

Experimental Observations

The function of G1677 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. The phenotypeof the 35S::G1677 transgenics was wild-type in morphology.

RT-PCR analysis of the endogenous levels of G1677 indicated that thisgene was expressed in most tissues tested, although at very low levels.This gene was not induced in leaf tissue in response to anystress-related condition tested.

G1677 overexpressing plants were found to have decreased seed oil andincreased seed protein content compared to wild-type plants.

Potential Applications

G1677 or equivalog overexpression may be used to alter oil and seedprotein content in plants.

G1749 (SEQ ID NO: 321)

Published Information

G1749 corresponds to gene At2g20350 (AAD21753).

Experimental Observations

The function of G1749 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter.

Overexpression of G1749 induced chlorosis and death of large patches oftissue in the aerial part of the plant, indicating that it might beinfluencing programmed cell death, perhaps in pathways that are usuallypart of senescence or of the disease response. At early stages ofdevelopment, 35S::G1749 seedlings appeared normal. However, towards theend of the rosette phase, these plants showed disorganized phyllotaxyand displayed rather broad flat leaves with short petioles. Randomlydistributed yellow specks and patches of chlorotic tissue became visibleat around this time; later these patches frequently developed intosizeable senesced regions covering large portions of the leaves.Additionally, similar effects were noted in the inflorescence, affectingcauline leaves, flower buds, and siliques. In severely affected plants,the entire inflorescence tips became brown and withered away withoutproducing seeds. These effects were displayed by almost all of the T1plants, and were visible in two independent batches of transformants,grown several months apart in separate locations.

Lines with the strongest phenotypes were completely infertile andsenesced without setting seed. Three lines with a milder phenotype,which had produced some seed, were therefore selected for furtheranalysis. All three T2 populations displayed the phenotypes to someextent, but these were weaker than were those shown by the majority ofT1 plants.

G1749 was specifically expressed in flower and silique tissues, and wasnot ectopically induced by any of the conditions tested.

Potential Applications

G1749 or its equivalogs could be used to trigger cell death, andtherefore to influence or control processes in which cell death plays arole. For example, if G1749 is an effective and rapid switch for celldeath programs, it could be used to block pathogen infection bytriggering it in infected cells and block spread of the disease.

G1749 or its equivalogs could also be used to either accelerate or slowsenescence of different plant organs. Although leaf senescence isthought to be an evolutionary adaptation to recycle nutrients, theability to control senescence in an agricultural setting has significantvalue. For example, a delay in leaf senescence in some maize hybrids isassociated with a significant increase in yields and a delay of a fewdays in the senescence of soybean plants can have a large impact onyield. Delayed flower senescence may also generate plants that retaintheir blossoms longer and this may be of potential interest to theornamental horticulture industry.

G1750 (SEQ ID NO: 323)

Published Information

G1750 was identified in the sequence of BAC clone T13J8; it correspondsto gene At4g27950.

Experimental Observations

The function of G1750 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1750 resulted in a significant increase in oil content in seeds, asmeasured by NIR. The increase in seed oil content was observed in twoindependent T2 lines, and was not accompanied by a substantial decreasein seed protein content. Otherwise, G1750 overexpressors behavedsimilarly to wild-type controls in all biochemical assays performed. Noalterations were detected in the T2 35S::G1750 plants in thephysiological analyses that were performed.

However, overexpression of G1750 caused alterations in plant growth anddevelopment. 35S::G1750 T1 plants showed a reduction in size, andapproximately 50% were extremely tiny, infertile, and sometimes hadpremature leaf senescence. Seed was obtained from only the T1 plantswith a weaker phenotype. Given the detrimental effects of G1750overexpression, transgenics in which the gene is regulated by a tissuespecific promoter, in particular a seed specific one, could beparticularly useful to study the gene's functions and utilities.

G1750 was ubiquitously expressed. G1750 expression levels may have beenaltered by a variety of environmental or physiological conditionsincluding SA.

Potential Applications

G1750 or its equivalogs could be used to increase seed oil content incrop plants.

G1756 (SEQ ID NO: 325)

Published Information

G1756 corresponds to gene AT4g23550 (CAB79310).

Closely Related Genes from Other Species

G1756 shows sequence similarity with known genes from other plantspecies within the conserved WRKY domain.

Experimental Observations

G1756 (SEQ ID NO:325) was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1756 caused alterations in plant growth and development, reducingoverall plant size and fertility. In addition, 35S::G1756 overexpressinglines showed more disease symptoms following inoculation with a low doseof the fungal pathogen Botrytis cinerea compared to the wild-typecontrols. G1756 was ubiquitously expressed and transcript levels werealtered by a variety of environmental or physiological conditions; G1756expression can be induced by auxin, cold, and Fusarium.

Potential Applications

As G1756 is likely to be involved in the disease response, it or itsequivalogs could be used to manipulate this response.

G1765 (SEQ ID NO: 327)

Published Information

G1765 was first identified in the sequence of the BAC clone F23E6,GenBank accession numberAC006580, released by the Arabidopsis GenomeInitiative.

Closely Related Genes from Other Species

A cDNA clone NF085A08EC from elicited cell culture of Medicagotruncatula is closely related to G1765.

Experimental Observations

The full length sequence of G1765 was experimentally confirmed. Thefunction of G1765 was analyzed using transgenic plants in which G1765was expressed under the control of the 35S promoter. The phenotype ofthese transgenic plants was wild-type in all assays performed with theexception of biochemical assays. Alterations in the leaf cell wallpolysaccharide composition were observed in plants that overexpressG1765. In one line, an increase in the percentage of rhamnose wasdetected. In another line, an increase in the percentage of mannose wasdetected. Otherwise, G1765 overexpressors behave similarly to wild-typecontrols in all biochemical assays performed.

RT-PCR analysis was used to determine the endogenous levels of G1765 ina variety of tissues and under a variety of environmental stress-relatedconditions. G1765 was primarily expressed at low levels in roots,flowers and rosette leaves. No expression was detected in shoots,embryos, siliques and germinated seedlings. RT-PCR data also indicated amoderate induction of G1765 transcripts accumulation upon auxin andFusarium treatments.

As measured by NIR, G1765 overexpressors had altered seed oil contentcompared to wild-type plants.

Potential Applications

G1765 or its equivalogs overexpression may be used to alter seed oilcontent, which may be very important for the nutritional value andproduction of various food products

G1777 (SEQ ID NO: 329)

Published Information

G1777 was identified as a gene in the sequence of Arabidopsis chromosomeII, section 93 using clone F7H1 (Accession Number AC007134), released byThe Institute for Genomic Research.

Closely Related Genes from Other Species

G1777 shows some homology to non-Arabidopsis proteins within theconserved RING finger domain.

Experimental Observations

G1777 (SEQ ID NO: 329) was analyzed using transgenic plants in whichG1777 was expressed under the control of the 35S promoter.Overexpression of G1777 in Arabidopsis resulted in an increase in seedoil content and a decrease in seed protein content in two T2 lines.G1777 was expressed in all examined tissue of Arabidopsis. G1777 wasinduced by auxin and ABA treatment, and by heat stress.

Potential Applications

G1777 or its equivalogs have utility in manipulating seed oil andprotein content.

G1792 (SEQ ID NO: 331)

Published Information

G1792 was identified in the sequence of BAC clone K14B15 (AB025608, geneK14B15.14).

Closely Related Genes from Other Species

G1792 shows sequence similarity, outside the conserved AP2 domain, witha portion of a predicted protein from tomato, represented by ESTsequence AI776626 (AI776626 EST257726 tomato resistant, CornellLycopersicon esculentum cDNA clone cLER19A14, mRNA sequence).

Experimental Observations

G1792 (SEQ ID NO: 331) was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G1792plants were more tolerant to the fungal pathogens Fusarium oxysporum andBotrytis cinerea and showed fewer symptoms after inoculation with a lowdose of each pathogen. This result was confirmed using individual T2lines. The effect of G1792 overexpression in increasing tolerance topathogens received further, incidental confirmation. T2 plants of two35S::G1792 lines had been growing in a room that suffered a seriouspowdery mildew infection. For each line, a pot of six plants was presentin a flat containing nine other pots of lines from unrelated genes. Ineither of the two different flats, the only plants that were free frominfection were those from the 35S::G1792 line. This observationsuggested that G1792 overexpression might be used to increase resistanceto powdery mildew. Additional experiments confirmed that 35S::G1792plants showed increased tolerance to Erysiphe. G1792 was ubiquitouslyexpressed, but appeared to be induced by salicylic acid.

35S::G1792 overexpressing plants also showed more tolerance to growthunder nitrogen-limiting conditions. In a root growth assay underconditions of limiting N, 35S::G1792 lines were slightly less stunted.In a germination assay that monitored the effect of C on N signalingthrough anthocyanin production on high sucrose plus and minus glutaminethe 35S::G1792 lines made less anthocyanin on high sucrose plusglutamine, suggesting that the gene can be involved in the plantsability to monitor their carbon and nitrogen status.

G1792 overexpressing plants showed several mild morphologicalalterations: leaves were dark green and shiny, and plants bolted,subsequently senesced, slightly later than wild-type controls. Among theT1 plants, additional morphological variation (not reproduced later inthe T2 plants) was observed: many showed reductions in size as well asaberrations in leaf shape, phyllotaxy, and flower development.

Potential Applications

G1792 or its equivalogs can be used to engineer pathogen-resistantplants. In addition, it can also be used to improve seedling germinationand performance under conditions of limited nitrogen.

Potential utilities of this gene or its equivalogs also includeincreasing chlorophyll content allowing more growth and productivity inconditions of low light. With a potentially higher photosynthetic rate,fruits could have higher sugar content. Increased carotenoid contentcould be used as a nutraceutical to produce foods with greaterantioxidant capability.

G1792 or its equivalogs could be used to manipulate wax composition,amount, or distribution, which in turn could modify plant tolerance todrought and/or low humidity or resistance to insects, as well as plantappearance (shiny leaves). In particular, it would be interesting to seewhat the effect of increased wax deposition on leaves of a plant likecotton would do to drought resistance or water use efficiency. Apossible application for this gene might be in reducing the wax coatingon sunflower seeds (the wax fouls the oil extraction system duringsunflower seed processing for oil). For this purpose, antisense orco-suppression of the gene in a tissue specific manner might be useful

G1793 (SEQ ID NO: 333)

Published Information

G1793 corresponds to gene MOE17.15 (BAB02492).

Experimental Observations

The function of G1793 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1793 produced alterations in cotyledon morphology and a mildreduction in overall plant size. Eight 35S::G1793 primary transformantswere obtained. Initially, these plants displayed abnormal long,elongated cotyledons. At later stages, the plants were all rather small,and in some cases slow growing, compared to controls. Inflorescenceswere often thin and, in 2/8 lines, carried flowers with manynon-specific abnormalities, including changes in organ size and number,and poor pollen production. All T1 plants showed moderate levels oftransgene expression (determined by RT-PCR).

G1793 overexpressors produced more seed oil than control plants.

G1793 expression was detected in a variety of tissues (root, flower,embryo, silique, and germinating seedling), and, except for heat stress,did not appear to be significantly induced by any of the conditionstested.

Potential Applications

G1793 or its equivalogs may be used to increase seed oil in plant seeds,which might be used to increase seed oil yield, and increase the caloriccontent of food for humans and animal feeds.

G1794 (SEQ ID NO: 335)

Published Information

G1794 corresponds to gene MVP7.8 (BAB10308.1).

Experimental Observations

The function of G1794 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter.

Overexpression of G1794 caused multiple alterations in plant growth anddevelopment, as well as in the plant's behavior in some of thephysiological analyses that were performed.

35S::G1794 plants showed modified branching patterns, and a reduction inapical dominance, which resulted in them having a shorter, more bushystature than wild type. Additionally, G1794 overexpression producedchanges in hypocotyl development. The loss of apical dominance wasnoticeable at the switch to flowering, when large numbers of secondaryshoots developed prematurely from axils of primary rosette leaves. Inthe most extreme cases, the shoots had very short internodes, giving theinflorescence a very bushy appearance. These shoots were often very thinand flowers were relatively small and poorly fertile. At later stages,many plants appeared very small and had a low seed yield compared towild type. Similar effects on inflorescence development were noted ineach of three T2 populations examined. Additionally, the T2 seedlingswere noted to have long thick hypocotyls and a decrease in root lengthcompared to controls.

Overexpression of G1794 in Arabidopsis resulted in an increase in leafglucosinolate M39480 in three T2 lines.

In the physiological analyses, it was noted that 35S::G1794 T2 seedlingsexhibited an altered hypocotyl structure, an altered light responsephenotype, and an enhanced sensitivity to osmotic stress and nitrogendepletion. All G1794 overexpressing transgenic lines showed thick,bulbous hypocotyls in the seedling stage as well as partiallyde-etiolated phenotype, the seedling displaying open and slightlyexpanded cotyledons when grown in the dark. The enhanced sensitivity toosmotic stress was observed in all three G1794 transgenic linesfollowing a root growth assay performed on high PEG containing media.Similarly, all three G1794 transgenic lines appear more sensitive togrowth on nitrogen depleted media. However, in all cases the G1794transgenic seedlings grew less vigorously than the wild-type controlsand that could contribute to their enhanced sensitivity to stress in theroot growth assays.

The branching and plant architecture phenotypes observed in 35S::G1794lines resembled phenotypes observed for three other AP2/EREBP genes:G865, G1411, and G2509. These three genes form a small clade within thelarge AP2/EREBP family, and G1794, although not belonging to the clade,is one of the AP2/EREBP genes closest to it in the phylogenetic tree. Itis thus possible that all these genes are related in function.

G1794 was ubiquitously expressed, and was induced by several stressconditions, in particular by osmotic stress.

Potential Applications

G1794 or its equivalogs could be used to manipulate plant architectureand development.

G1794 or its equivalogs could be used to alter a plant's response towater deficit conditions and therefore, could be used to engineer plantswith enhanced tolerance to drought, salt stress, and freezing.

Overexpression of G1794 or its equivalogs may also induce changes inglucosinolate content.

G1794 modified light response and thus it or its equivalogs may beuseful for modifying plant growth or development, for example,photomorphogenesis in poor light, or accelerating flowering time inresponse to various light intensities, quality or duration to which anon-transformed plant would not similarly respond. Elimination ofshading responses may allow increased planting densities with subsequentyield enhancement.

G1804 (SEQ ID NO: 337)

Published Information

G1804 was identified in the sequence of BAC F9C22, GenBank accessionnumber AC006921, released by the Arabidopsis Genome Initiative. Duringthe course of its functional analysis, the G1804 sequence was publishedas the Arabidopsis ABI5 gene, which, when knocked out, causespleiotropic effects on responses to the hormone abscisic acid(Finkelstein et al. (1990) Plant Cell 12: 599-609). In addition, G1804was deposited in the NCBI database as DPBF1. DPBF1 is an Arabidopsisembryo bZIP transcription factor that interacts with the lateembryogenesis Dc3 gene promoter of sunflower (Kim et al: (1991)Unpublished deposit in the NCBI database).

ABI5 can be induced by ABA, drought and high salt stress in embryos(Lopez-Molina, et al (2001) Proc. Natl. Acad. Sci. USA 98: 4782-4787).Its overexpression causes ABA hypersensitivity and delayed germination(Lopez-Molina, et al (2001) Proc. Natl. Acad. Sci. USA 98: 4782-4787),and it is postulated but not shown that ABI5 could be used to engineerdrought hardiness into seeds or plants.

Closely Related Genes from Other Species

G1804 is likely to be a homolog of the sunflower Dc3 promoter-bindingfactor-1 (DPBF-1; accession number AF001453) which also interacts withthe Dc3 gene promoter of sunflower (Kim et al, 1991).

Experimental Observations

The boundaries of G1804 were experimentally determined and the functionof G1804 was analyzed using transgenic plants in which this gene wasexpressed under the control of the 35S promoter. Plants overexpressingG1804 were later flowering and more sensitive to glucose in agermination assay. G1804 appeared to be preferentially expressed inembryos and flowers, and induced by auxin treatment. The expressionpattern and annotation of G1804 correlated well with the informationregarding DPB1 in the NCBI database and published information on ABI5.

Potential Applications

G1804 or its equivalogs may be used to modify sugar sensing andsource-sink relationships in plants.

Manipulating the sugar signal transduction pathway may lead to alteredgene expression to produce plants with desirable traits. In particular,manipulation of sugar signal transduction pathways could be used toalter source-sink relationships in seeds, tubers, roots and otherstorage organs leading to increase in yield.

G1804 or its equivalogs may also have a utility in modifying floweringtime, and the promoter of G1804 may have some utility as an embryospecific promoter.

G1818 (SEQ ID NO: 339)

Published Information

G1818 is a member of the Hap5-like subfamily of CCAAT-box bindingtranscription factors. G1818 was identified in the sequence of P1clone:MBA10, GenBank accession number AB025619, released by theArabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G1818 was determined. The function of this genewas analyzed using transgenic plants in which G1818 was expressed underthe control of the 35S promoter. The phenotype of these transgenicplants was wild-type in all physiological assays performed. However,overexpression of G1818 delayed the timing of flowering and producedalterations in leaf shape. The leaves appeared to be flatter thanwild-type leaves at all stages of development. In addition, G1818overexpression resulted in higher seed protein content in two out of thethree lines.

G1818 expression was detected in embryo, flower and silique tissue byRT-PCR. Expression of G1818 was also detected in leaf tissue followingcold and auxin treatments. However, no cold related phenotypes wereobserved.

Potential Applications

G1818 or its equivalogs may be used to manipulate flowering time.

Additionally, a major concern is the escape of transgenic pollen fromGMOs to wild species or so-called organic crops. Genes such as G1818 orits equivalogs that prevent vegetative transgenic crops from floweringwould eliminate this worry.

G1818 or its equivalogs could also be used to increase seed proteinamounts and/or alter seed protein composition, which could impact yieldas well as the nutritional value and production of various foodproducts. An increase in storage proteins is desirable for example incorn seeds to increase the nutritional value of the meal. Seed proteinsplay a central role in human and animal diets and represent amultibillion dollar market worldwide.

G1820 (SEQ ID NO: 341)

Published Information

G1820 is a member of the Hap5 subfamily of CCAAT-box-bindingtranscription factors. G1820 was identified as part of the BAC cloneMBA10, accession number AB025619 released by the Arabidopsis Genomesequencing project.

Closely Related Genes from Other Species

G1820 is closely related to a soybean gene represented by EST335784isolated from leaves infected with Colletotrichum trifolii. Similaritybetween G1820 and the soybean gene extends beyond the signature motif ofthe family to a level that would suggest the genes are orthologous.Therefore the gene represented by EST335784 may have a function and/orutility similar to that of G1820.

Experimental Observations

The complete sequence of G1820 was determined. The function of this genewas analyzed using transgenic plants in which G1820 was expressed underthe control of the 35S promoter. G1820 overexpressing lines showed moretolerance to salt stress in a germination assay. They also showedinsensitivity to ABA, with the three lines analyzed showing thephenotype. The salt and ABA phenotypes could be related to the plantsincreased tolerance to osmotic stress because in a severe waterdeprivation assay, G1820 overexpressors are, again, more tolerant.

Interestingly, overexpression of G1820 also consistently reduced thetime to flowering. Under continuous light conditions at 20-25 C, the35S::G1820 transformants displayed visible flower buds several daysearlier than control plants. The primary shoots of these plantstypically started flower initiation 1-4 leaf plastochrons sooner thanthose of wild type. Such effects were observed in all three T2populations and in a substantial number of primary transformants.

When biochemical assays were performed, some changes in leaf fames weredetected. In one line, an increase in the percentage of 18:3 and adecrease in 16:1 were observed. Otherwise, G1820 overexpressors behavedsimilarly to wild-type controls in all biochemical assays performed. Asdetermined by RT-PCR, G1820 was highly expressed in embryos andsiliques. No expression of G1820 was detected in the other tissuestested. G1820 expression appeared to be induced in rosette leaves bycold and drought stress treatments, and overexpressing lines showedtolerance to water deficit and high salt conditions.

One possible explanation for the complexity of the G1820 overexpressionphenotype is that the gene is somehow involved in the cross talk betweenABA and GA signal transduction pathways. It is well known that seeddormancy and germination are regulated by the plant hormones abscisicacid (ABA) and gibberellin (GA). These two hormones act antagonisticallywith each other. ABA induces seed dormancy in maturing embryos andinhibits germination of seeds. GA breaks seed dormancy and promotesgermination. It is conceivable that the flowering time and ABAinsensitive phenotypes observed in the G1820 overexpressors are relatedto an enhanced sensitivity to GA, or an increase in the level of GA, andthat the phenotype of the overexpressors is unrelated to ABA. InArabidopsis, GA is thought to be required to promote flowering innon-inductive photoperiods. However, the drought and salt tolerantphenotypes would indicate that ABA signal transduction is also perturbedin these plants. It seems counterintuitive for a plant with salt anddrought tolerance to be ABA insensitive since ABA seems to activatesignal transduction pathways involved in tolerance to salt anddehydration stresses. One explanation is that ABA levels in the G1820overexpressors are also high but that the plant is unable to perceive ortransduce the signal.

G1820 overexpressors also had decreased seed oil content and increasedseed protein content compared to wild-type plants

Potential Applications

G1820 affects ABA sensitivity, and thus when transformed into a plantthis transcription factor or its equivalogs may diminish cold, drought,oxidative and other stress sensitivities, and also be used to alterplant architecture, and yield.

The osmotic stress results indicate that G1820 or its equivalogs couldbe used to alter a plant's response to water deficit conditions and canbe used to engineer plants with enhanced tolerance to drought, saltstress, and freezing. Evaporation from the soil surface causes upwardwater movement and salt accumulation in the upper soil layer where theseeds are placed. Thus, germination normally takes place at a saltconcentration much higher than the mean salt concentration of in thewhole soil profile. Increased salt tolerance during the germinationstage of a crop plant would impact survivability and yield.

G1820 or its equivalogs could also be used to accelerate flowering time.

G1820 or its equivalogs may be used to modify levels of saturation inoils.

G1820 or its equivalogs may be used to seed protein content.

The promoter of G1820 could be used to drive seed-specific geneexpression.

Potential Applications

G1820 or equivalog overexpression may be used to alter seed proteincontent, which may be very important for the nutritional value andproduction of various food products

G1836 (SEQ ID NO: 343)

Published Information

G1836 was identified in the sequence of BAC F14123, GenBank accessionnumber AC007399, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G1836 was determined. The function of this genewas analyzed using transgenic plants in which G1836 was expressed underthe control of the 35S promoter. Morphologically, the plants weresomewhat more pale than the wild-type controls. This observation did nottranslate into a detectable difference in the chlorophyll a orchlorophyll b content in these transgenics (see biochemistry data).Overexpression of G1836 affected the plants' ability to tolerate highconcentrations of salt in a germination assay. All of the lines showedgreater expansion of the cotyledons when seeds are germinated on MSmedia containing high concentrations of NaCl, indicating they had moretolerance to salt stress compared to the wild-type controls. There wasno enhanced tolerance to high salt in older seedlings in a root growthassay. This was not unexpected because salt tolerance in the twodevelopmental stages in often uncoupled in nature indicating mechanisticdifferences.

G1836 overexpression also resulted in plants that were more droughttolerant than wild-type control plants.

Expression of G1836 was also repressed by Erysiphe orontii infection.

Potential Applications

G1836 or its equivalogs could be used to increase plant tolerance todrought tolerance and soil salinity during germination, or at theseedling stage.

G1838 (SEQ ID NO: 345)

Published Information

G1838 corresponds to gene K21L13.1 (BAA98170).

Experimental Observations

The function of G1838 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1838 caused alterations in plant growth and development: 35S::G1838plants were smaller than wild type, often possessed curled, darker greenleaves, and showed reduced fertility. 35S::G1838 primary transformantsfrequently displayed seedling abnormalities such as elongatedcotyledons. Later, many of the lines were small, grew slowly and formedhighly abnormal leaves. These structures were often narrow, darkergreen, contorted, or had strange horn like growths on their surfaces.Inflorescences were typically short, poorly developed, and carriedinfertile flowers that had small, contorted, or missing organs. Due tothese deficiencies, the many of T1 plants formed very few seeds. Threelines that showed a relatively weak phenotype were selected for furtherstudy. Plants overexpressing G1838 were found to produce more seed oilthan wild-type plants.

G1838 was ubiquitously expressed, and did not appear to be significantlyinduced by any of the conditions tested.

G1838 belongs to the AP2 subfamily of the AP2/EREBP family. It washypothesized that genes of this subfamily would be involved in plantdevelopmental processes (Riechmann et al. (1998) Biol Chem. 379:633-646)which could thus explain the pleiotropic nature of the phenotypesobserved in 35S::G1838 plants.

Potential Applications

G1838 or its equivalogs may be used for increasing seed oil productionin plants, which would be of nutritional value for food for humanconsumption as well as animal feeds.

G1841 (SEQ ID NO: 347)

Published Information

G1841 corresponds to gene MPF21.9 (BAB10294). No information isavailable about the function(s) of G1841.

Experimental Observations

The function of G1841 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter.

Most significantly, overexpression of G1841 markedly reduced the time toflowering. This early flowering phenotype was consistently observed overmultiple plantings for each of the three T2 lines, and in a majority ofprimary transformants. 35S::G1841 plants appeared slightly pale and hadrather flat leaves compared to wild-type controls, but no other obviousmorphological alterations. In continuous light conditions, 35S::G1841plants produced flower buds up to five days earlier than wild-typecontrols. Interestingly, in repeat sowings, the plants actually appearedto grow slightly faster than controls: although they switched to makingflower buds several days early, they had a similar number of primaryrosette leaves to wild type.

In addition to showing accelerated flowering under 24 hours light,plants from all three T2 populations produced flowers up to 2 weeksearlier than controls under a 12 hour photoperiod. 35S::G1841 seed alsoshowed a slight tolerance to heat in a germination assay compared towild-type controls.

G1841 appeared to be specifically expressed in floral tissues (includingembryo and silique), and to be ectopically induced by heat stress. ThatG1841 was specifically expressed in floral tissues but yet canconsistently influence the floral transition when overexpressed, mightappear paradoxical, but this is a phenomenon that has already beenobserved for other transcription factor genes, for example theArabidopsis homeobox gene FWA (Soppe et al. (2000) Mol. Cell. 6:791-802). That G1841 expression was induced by heat lends support to theresult that G1841 overexpression can improve germination under heatstress.

Potential Applications

G1841 or its equivalogs could be used to modify flowering time(accelerating the switch to flowering by overexpression), as well as toimprove seed germination under heat stress. The promoter of G1841 couldbe used to direct heat inducible gene expression in transgenic plants.In general, a wide variety of applications exist for systems that eitherlengthen or shorten the time to flowering.

G1842 (SEQ ID NO: 349)

Published Information

G1842 corresponds to F15O5.2 (BAA97510). The high level of sequencesimilarity between G1842 and FLOWERING LOCUS C (Michaels and Amasino,1999; Sheldon et al., 1999) has been previously described (Ratcliffe etal. (2001) Plant Physiol. 126:122-132).

Experimental Observations

G1842 was recognized as a gene highly related to Arabidopsis FLOWERINGLOCUS C (FLC; Michaels et al. (1999) Plant Cell 11, 949-956; Sheldon etal. (1999) Plant Cell 11, 445-458), and to MADS AFFECTING FLOWERING 1(Ratcliffe et al. (2001) Plant Physiol. 126:122-132). FLC acts as arepressor of flowering (Michaels et al. (1999) Plant Cell 11, 949-956;Sheldon et al. (1999) Plant Cell 11, 445-458). Similarly, G157/MAF1 cancause a delay in flowering time when overexpressed (Ratcliffe et al.(2001) Plant Physiol. 126:122-132.

The function of G1842 was studied using transgenic plants in which thisgene was expressed under the control of the 35S promoter. Overexpressionof G1842 reduced the time to flowering in the Columbia background. Noconsistent alterations were detected in 35S::G1842 plants in thephysiological and biochemical analyses that were performed.

Early flowering was observed in 13/21 35S::G1842 primary transformants:under continuous light conditions, these plants produced flower budsapproximately 1 week earlier than controls. A comparable phenotype wasalso noted in the T2 populations from each of the three lines examined.In a separate experiment, the 35S::G1842 transgene was transformed intoStockholm (a late flowering, vernalization-sensitive ecotype). Acomparable result was observed to that seen for Columbia: approximately50% of 35S::G1842 Stockholm plants flowered earlier than wild-typecontrols.

Although G1842 is highly related in sequence to G157, G859, and FLC, itsoverexpression reduced the time to flowering, whereas overexpression ofG157, G859, and FLC often caused a delay in flowering. In other words,whereas the function of G157, G859, and FLC appeared to repressflowering, G1842 was an activator of that process.

Potential Applications

G1842 or its equivalogs could be used to alter flowering time.

G1843 (SEQ ID NO: 351)

Published Information

G1843 corresponds to F1505.3 (BAA97511). There is no literaturepublished on G1843, except our own (Ratcliffe et al. (2001) PlantPhysiol. 126:122-132). G1843 belongs to a group of five ArabidopsisMADS-box genes that are highly related to FLC (G1759), a repressor ofthe floral transition, and that we have called MADS AFFECTING FLOWERING1-5 (Ratcliffe et al. (2001) Plant Physiol. 126:122-132). The publishedreport describes functional data for only MAF1 (G157), but the sequencesimilarity among all the members of the group is noted.

Experimental Observations

The function of G1843 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1843 caused alterations in plant growth and development, inparticular a severe reduction in overall plant size, prematuresenescence, and early flowering. That G1843 caused an effect inflowering time was expected because of its sequence similarity to G1759(FLC), G157 (MAF1), and G859, G1842, and G1844. However, in contrast toall these other genes, which when overexpressed can alter flowering time(either delay or accelerate, depending on the gene) without severe sideeffects on the plant, overexpression of G1843 was severely detrimental.

Primary transformants for 35S::G1843 were consistently small, showedstunted growth, and formed poorly developed inflorescences that yieldedrelatively few seeds. The most severely affected of these plants werevery small, and died at early stages of development. Approximately 50%of the 35S::G1843 transformants were also markedly early flowering anddisplayed visible flower buds 1-7 days earlier than any of the wild-typecontrols. Most notably, the leaves of 35S::G1843 transformantsfrequently senesced prematurely. A total of six T2 lines weremorphologically examined; all exhibited (to varying extents) comparablephenotypes to those observed in the T1 generation, showing prematuresenescence and stunted growth. Due to these deleterious effects,however, an accurate determination of flowering time was difficult tomake in the T2 generation.

The deleterious effects caused by G1843 overexpression were also notedin the physiological analyses that were performed: in general, the G1843overexpressing lines showed reduced seedling vigor and were palecompared to wild-type controls. 35S::G1843 plants behaved otherwise likewild-type controls in the physiological assays.

No alterations were detected in 35S::G1843 plants in the biochemicalanalyses that were performed.

G1843 was ubiquitously expressed and did not appear to be significantlyinduced by any of the conditions tested.

Potential Applications

G1843 or its equivalogs could be used to manipulate flowering time.

G1852 (SEQ ID NO: 353)

Published Information

G1852 was identified by amino acid sequence similarity to plant andmammalian ankyrin-repeat proteins. It is found in the sequence of thechromosome 4 BAC F15P23 (GenBank accession number AF128392.1GI:4325336), released by the Arabidopsis Genome Initiative. Thetranslational start and stop codons were correctly predicted. G1852 hasno distinctive features other than the presence of a 33-aa repeatedankyrin element known for protein-protein interaction, in the C-terminusof the predicted protein.

Closely Related Genes from Other Species

A comparison of the amino acid sequence of G1852 with entries availablefrom GenBank shows strong similarity with plant ankyrins of severalspecies (Malus domestica, Solanum tuberosum, Oryza sativa, Gossypiumarboreum, Medicago truncatula, Glycine max, Lycopersicon esculentum,Pinus taeda, Lotus japonicus and Gossypium hirsutum).

Experimental Observations

G1852 (SEQ ID NO:353) was analyzed through its ectopic overexpression inplants. Analysis of the endogenous level of G1852 transcripts by RT-PCRrevealed expression in all tissues tested. G1852 expression was inducedin response to ABA, heat and drought treatment. 35S::G1852 overexpressorplants were more tolerant to osmotic stress in a root growth assay onPEG (polyethylene glycol)-containing media compared with wild-typecontrols. Seedlings were larger and had more root growth.

Potential Applications

G1852 or its equivalogs can be used to alter a plant's response to waterdeficit conditions and therefore, be used to engineer plants withenhanced tolerance to drought, salt stress, and freezing.

G1863 (SEQ ID NO: 355)

Published Information

G1863 was identified by amino acid sequence similarity to the riceGrowth-regulating-factor1 (GRF1), with a potential role in theregulation of stem growth in rice (Knapp et al (2000) Plant Physiol.122: 695-704) It is found in the sequence of chromosome II section 199of 255 (GenBank accession AC006919.5 GI:6598632), released by theArabidopsis Genome Initiative. The transcription start/stop codon wascorrectly predicted.

Experimental Observations

Tissue distribution of G1863 transcripts reveals that this gene wasexpressed constitutively, but with a reduced expression level in shoots.No changes in G1863 expression was observed in the biotic/abiotictreatments examined Physiological analysis of G1863 null mutant showedan increase in sensitivity to germination in high salt condition. Thereduction in germination and seedling vigor was specific to NaCl-treatedplants. G1863 null mutants responded like wild-type control in droughtand osmotic essays. This phenotype was confirmed in a follow-upexperiment. G1863 mutation had no apparent effect on plant developmentand morphology or biochemical profile.

Potential Applications

G1863 or its equivalogs could be used to modify plant tolerance to soilsalinity during the germination stage.

G1880 (SEQ ID NO: 357)

Published Information

G1880 was identified in the sequence of Chromosome 2, GenBank accessionnumber AC006532, released by the Arabidopsis Genome Initiative

Closely Related Genes from Other Species

Closely related sequences to G1880 include a putative zinc fingerprotein in rice (GenBank accession number 10934090), a predicted proteinin tomato (9858780), and a cDNA sequence from M. truncatula (AW685627)Similarity between G1880 and these genes extends beyond the signaturemotif of the family to a level that would suggest the genes areorthologous.

Experimental Observations

G1880 was expressed throughout the plant, with significantly lowerlevels of expression in siliques. It was induced by auxin, ABA, heat,and salt, and possibly repressed by Erysiphe infection. A linehomozygous for a T-DNA insertion in G1880 was used to determine thefunction of this gene. These plants showed fewer disease symptomsfollowing inoculation with a low dose of the fungal pathogen Botrytiscinerea in two separate experiments. No altered phenotypes were observedin any morphological or biochemical assay.

Potential Applications

Since G1880 activity has been shown to affect the response of transgenicplants to the fungal pathogen Botrytis cinerea, G1880 could be used tomanipulate the defense response in order to generate pathogen-resistantplants.

G1895 (SEQ ID NO: 359)

Published Information

G1895 was identified as a gene in the sequence of the BAC T24P13(Accession Number AC006535), released by the Arabidopsis thaliana GenomeCenter.

Experimental Observations

The function of G1895 was analyzed using transgenic plants in whichG1895 was expressed under the control of the 35S promoter.Overexpression of G1895 delayed the onset of flowering in Arabidopsis byaround two to three weeks under continuous light conditions, althoughthis phenotype was observed only at low frequency. In all otherphysiological and biochemical assays, 35S::G1895 plants appearedidentical to controls. G1895 was expressed in all tissues and thehighest levels of expression were found in flowers, rosette leaves, andembryos. In rosette leaves, G1895 was be induced by auxin, ABA, and bycold stress.

Potential Applications

G1895 or its equivalogs might be used to engineer plants with a delayedflowering time.

G1902 (SEQ ID NO: 361)

Published Information

G1902 corresponds to the Arabidopsis adof2 gene (Accession numberAB017565).

Experimental Observations

The function of G1902 was analyzed using transgenic plants in whichG1902 was expressed under the control of the 35S promoter.Overexpression of G1902 produced deleterious effects on plant growth anddevelopment. All 35S::G1902 primary transformants appeared markedlysmall throughout the life cycle, produced thin inflorescence stems, andshowed poor fertility compared to wild type. Comparable effects wereobserved, to varying extents, in each of the three T2 populationsexamined Additionally, plants from one of the T2 populations showedaberrant flowers with narrow perianth organs and short stamens. It isnoteworthy that all three T2 populations showed an apparent deficit ofkanamycin resistant seedlings, indicating that G1902 expression may havebeen lethal at high dosages, or that the NPT marker gene was beingsilenced.

35S::G1902 plants produced more seed oil than wild-type plants. G1902was expressed in all tissues, and was induced by auxin, ABA, heat anddrought stress.

Potential Applications

G1902 or its equivalogs may be used for increasing seed oil productionin plants, which would be of nutritional value for food for humanconsumption as well as animal feeds.

G1903 (SEQ ID NO: 363)

Published Information

G1903 was identified from the Arabidopsis genomic sequence, GenBankaccession number AC021046, based on its sequence similarity within theconserved domain to other DOF related proteins in Arabidopsis.

Experimental Observations

The function of this gene was analyzed using transgenic plants in whichG1903 was expressed under the control of the 35S promoter. Two linesshowed a significant decrease in seed protein content and an increase inseed oil content as assayed by NIR, otherwise the phenotype of thesetransgenic plants was wild-type in all other assays performed.

Gene expression profiling using RT/PCR showed that G1903 was expressedpredominantly in flowers, however it was almost undetected in roots andseedlings. Furthermore, there was no significant effect on expressionlevels of G1903 after exposure to environmental stress conditions.

Potential Applications

Seed proteins play a central role in human and animal diets andrepresent a multibillion dollar market worldwide. G1903 or itsequivalogs could be used to alter seed protein amounts and/orcomposition which could impact yield as well as the caloric content andthe nutritional value and production of various food products. Anincrease in storage proteins is desirable for example in corn seeds toincrease the nutritional value of the meal.

G1919 (SEQ ID NO: 365)

Published Information

G1919 was identified as a gene in the sequence of the P1 clone MBK5(Accession Number AB005234), released by the Kazusa DNA ResearchInstitute (Chiba, Japan).

Experimental Observations

The function of G1919 was analyzed using transgenic plants in whichG1919 was expressed under the control of the 35S promoter. 35S::G1919transformants displayed wild-type morphology at all stages ofdevelopment. However, plants overexpressing G1919 showed a greatertolerance to the fungal pathogen Botrytis cinerea than control plants.This phenotype has been confirmed by repeated experiment. No otheraltered phenotypes were observed in any of the physiological orbiochemical assays. G1919 was expressed at low levels in flowers, and athigher levels in embryos and siliques. G1919 was not significantlyinduced by any condition tested.

Potential Applications

Since G1919 activity has been shown to affect the response of transgenicplants to the fungal pathogen Botrytis cinerea, G1919 or its equivalogscould be used to manipulate the defense response in order to generatepathogen-resistant plants.

G1927 (SEQ ID NO: 367)

Published Information

G1927 was identified in the sequence of BAC F23M19, GenBank accessionnumber AC007454, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G1927 showed extensive sequence similarity to a NAC protein from tomato(BG350410).

Experimental Observations

G1927 (SEQ ID NO: 367) was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1927 in Arabidopsis resulted in plants that had an altered responseto pathogen. Plants overexpressing G1927 showed fewer disease symptomsfollowing infection with the fungal pathogen Sclerotinia sclerotiorumcompared with control plants. The experiment was repeated on individuallines, and all three lines showed the enhanced pathogen tolerancephenotype. G1927 expression appeared to be ubiquitous according toRT-PCR analysis.

Potential Applications

G1927 or its equivalogs can be used to manipulate the defense responsein order to generate pathogen-resistant plants.

G1930 (SEQ ID NO: 369)

Published Information

G1930 was identified in the sequence of P1 clone K13N2 (gene K13N2.7,GenBank protein accession number BAA95760).

Closely Related Genes from Other Species

G1930 shows sequence similarity, outside of the conserved AP2 and ABI3domains, to a predicted rice protein (GenBank accession numberBAB21218).

Experimental Observations

The function of G1930 was studied using transgenic plants in which thisgene was expressed under the control of the 35S promoter. G1930overexpressors were more tolerant to osmotic stress conditions. Theplants responded to high NaCl and high sucrose on plates with moreseedling vigor compared to wild-type control plants. In addition, anincrease in the amount of chlorophylls a and b in seeds of two T2 lineswas detected. However, constitutive expression of G1930 also produced avariety of morphological, physiological, and biochemical alterations.35S::G1930 T1 plants were generally small and developed spindlyinflorescences. The fertility of these plants was low and flowers oftenfailed to open or pollinate.

G1930 was ubiquitously expressed and did not appear to be induced by anyof the conditions tested.

Potential Applications

G1930 or its equivalogs could be used to increase germination underadverse osmotic stress conditions, which could impact survivability andyield. This gene could also be used to regulate the levels ofchlorophyll in seeds.

G1936 (SEQ ID NO: 371)

Published Information

The sequence of G1936 was obtained from the Arabidopsis genomesequencing project, GenBank accession number AB010072, based on itssequence similarity within the conserved domain to other PCF relatedproteins in Arabidopsis.

Experimental Observations

The function of G1936 was studied using a line homozygous for a T-DNAinsertion in the gene. The DNA insertion lies shortly before the ATGstart site of the coding sequence in the 5′ UTR region and is expectedto result in a null mutation.

G1936 knockout mutant plants showed more disease symptoms followinginoculation with the fungal pathogen Sclerotinia sclerotiorum. They alsoshowed more disease symptoms after inoculation with a low dose ofBotrytis cinerea compared to control plants.

As determined by RT-PCR, G1936 was uniformly expressed in all tissueswith exception of germinating seeds. Expression level of G1936 wasunchanged by any of the environmental conditions or pathogens infectionstested.

Potential Applications

Since G1936 transgenic plants have an altered response to the pathogensSclerotinia sclerotiorum and Botrytis cinerea, G1936 or its equivalogscould be used to manipulate the defense response in order to generatepathogen-resistant plants

G1944 (SEQ ID NO: 373)

Published Information

The sequence of G1944 was obtained from EU Arabidopsis sequencingproject, GenBank accession number AL049638, based on its sequencesimilarity within the conserved domain to other AT-Hook related proteinsin Arabidopsis.

Closely Related Genes from Other Species

G1944 protein shares a significant homology to Glycine max cDNA clones.Similarity between G1944 and the Glycine max cDNA clones extends beyondthe signature motif of the family to a level that would suggest thegenes are orthologous. Therefore the gene represented by cDNA clonesBE822274 and BE555817 may have a function and/or utility similar to thatof G1944. No further information is available about the cDNA clonesBE822274 and BE555817.

Experimental Observations

The sequence of G1944 was experimentally determined and the function ofG1944 was analyzed using transgenic plants in which G1944 was expressedunder the control of the 35S promoter.

Overexpression of G1944 reduced overall plant size and resulted inpremature senescence of rosette leaves.

Physiological assays revealed that seedlings from G1944 overexpressorlines were more severely stunted in an ethylene insensitivity assay whencompared to the wild-type controls. This result indicated that G1944 isinvolved in the ethylene signal transduction pathway. It is well knownthat ethylene is involved in the senescence process and therefore, thephenotype of premature senescence of rosette leaves could be related toa general sensitivity to ethylene signal transduction pathway.

As determined by RT-PCR, G1944 was expressed in most of tissues tested.Expression level of G1944 appeared to be induced by auxin and salicylicacid treatments.

Potential Applications

G1944 or its equivalogs, because of its effect on plant size and leafsenescence may be used to modify plant growth and development.

G1944 or its equivalogs could be used to alter senescence of differentplant organs. Although leaf senescence is thought to be an evolutionaryadaptation to recycle nutrients, the ability to control senescence in anagricultural setting has significant value. For example, a delay in leafsenescence in some maize hybrids is associated with a significantincrease in yields and a delay of a few days in the senescence ofsoybean plants can have a large impact on yield. Delayed flowersenescence may also generate plants that retain their blossoms longerand this may be of potential interest to the ornamental horticultureindustry.

G1946 (SEQ ID NO: 375)

Published Information

The heat shock transcription factor G1946 is a member of the class-AHSFs (Nover et al. (1996) Cell Stress Chaperones 1: 215-223)characterized by an extended HR-A/B oligomerization domain. G1946 isfound in the sequence of the chromosome 1, BAC F5D14 (GenBank accessionAC007767.3 GI:7549621), released by the Arabidopsis Genome Initiative.The translational start codon was incorrectly predicted.

Closely Related Genes from Other Species

A comparison of the amino acid sequence of G1946 with sequencesavailable from GenBank showed strong similarity with plant HSFs ofseveral species (Lycopersicon peruvianum, Medicago truncatula,Lycopersicon esculentum, Glycine max, Solanum tuberosum, Oryza sativaand Hordeum vulgare subsp. Vulgare).

Experimental Observations

G1946 (SEQ ID NO:375) was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G1946 resulted in accelerated flowering, with 35S::G1946transformants producing flower buds up to a week earlier than wild-typecontrols (24-hour light conditions). These effects were seen in 12/20primary transformants and in two independent plantings of each of thethree T2 lines Unlike many early flowering Arabidopsis transgenic lines,which are dwarfed, 35S::G1946 transformants often reached full-size atmaturity, and produced large quantities of seeds, although the plantswere slightly pale in coloration and had slightly flat leaves comparedto wild type. In addition, 35S::G1946 plants showed an altered responseto phosphate deprivation. Seedlings of G1946 overexpressors showed moresecondary root growth on phosphate-free media, when compared towild-type control. In a repeat experiment, all three lines showed thephenotype. Overexpression of G1946 in Arabidopsis also resulted in anincrease in seed glucosinolate M39501 in two T2 lines. An increase inseed oil and a decrease in seed protein were also observed in these twolines. G1946 was ubiquitously expressed, and did not appear to besignificantly induced or repressed by any of the biotic and abioticstress conditions tested, with the exception of cold, which repressedG1946 expression.

Potential Applications

G1946 or its equivalogs can be used to modify flowering time, as well asto improve the plant's performance in conditions of limited phosphate,and to alter seed oil, protein, and glucosinolate composition.

G1947 (SEQ ID NO: 377)

Published Information

The heat shock transcription factor G1947 is a member of the class-AHSFs (Nover et al. (1996) Cell Stress Chaperones 1: 215-223)characterized by an extended HR-A/B oligomerization domain. G1947 isfound in the sequence of the chromosome 5 P1 clone MQD19 (GenBankaccession AB026651.1 GI:4757407), released by the Arabidopsis GenomeInitiative. The start codon was incorrectly predicted in the publicannotation.

Experimental Observations

Analysis of the endogenous level of G1947 transcripts by RT-PCR revealeda constitutive expression, with the highest expression levels in rosetteleaves and the lowest in shoots and roots. G1947 expression appeared tobe induced by a variety of physiological or environmental conditions(auxin, ABA, heat, drought and osmotic stress).

A line homozygous for a T-DNA insertion in G1947 was used to analyze thefunction of this gene. The insertion point is 163 nucleotides downstreamfrom the initiation codon of G1947, and therefore should result in anull mutation.

G1947 mutant plants formed inflorescences that grew for an extendedperiod of time, and continued to generate flowers for substantiallylonger than wild-type controls. In G1947 mutant plants, siliquedevelopment was generally poor: they were very short and contained onlya few irregularly shaped seeds. Thus, the extended phase of flowerproduction observed in G1947 knockout mutant plants might have been theresult of poor fertility, because extended production of flowers anddelayed floral organ abscission is often seen in sterile Arabidopsismutants. The basis for the reduced fertility of G1947 knockout plantswas not apparent from the morphology of their flowers. In addition, someinconsistent effects on seedling size were noted for G1947 knockoutmutants. No size differences were noted between rosette stage G1947knockout plants and controls, although at late stages the G1947 knockoutplants appeared bushier than controls, probably due to continued growthof the inflorescence stems.

No altered phenotypes were observed for G1947 knockout plants in any ofthe physiological or biochemical assays performed.

Potential Applications

G1947 or its equivalogs could be used to engineer infertility intransgenic plants. G1947 may also have utility in engineering plantswith longer-lasting flowers for the horticulture industry.

G1948 (SEQ ID NO: 379)

Published Information

G1948 was identified by amino acid sequence similarity to plant andmammalian ankyrin-repeat proteins. It is found in the sequence of thechromosome 2, clones T8113, T30B22 (GenBank accession number AC002535.2GI:6598379), released by the Arabidopsis Genome Initiative. G1948 hasalso been referred to as CHAOS (CAO), acronym for CHLOROPHYLL A/BBINDING PROTEIN HARVESTING-ORGANELLE SPECIFIC (Klimyuk et al. (1999)Plant Cell. 11(1):87-99). The CAO protein contains ankyrin repeats inits central region, and a chromodomain in its carboxy terminal part.Chromodomains are usually found in chromatin-related proteins. However,the CAO protein was shown to be a plant-specific component of thechloroplast signal recognition particle pathway that is involved in LHCPtargeting (Klimyuk et al. (1999) Plant Cell 11:87-99).

Experimental Observations

The function of G1948 was analyzed through its ectopic overexpression inplants. Expression analysis by RT-PCR revealed a low/moderate expressionlevel of G1948 in all above-ground tissues, in agreement with previouslypublished observations (Klimyuk et al. (1999) Plant Cell 11:87-99).G1948 expression was not altered by any of the and biotic/abiotictreatments examined.

The characterization of G1948 overexpressor lines revealed increasedseed oil content relative to wild-type plants.

Potential Applications

G1948 or its equivalogs could used to increase seed oil content, whichwould be of value for modifying the nutritional value and caloriccontent of food for human consumption as well as animal feeds, and maybe of value in improving seed storage characteristics.

G1950 (SEQ ID NO: 381)

Published Information

G1950 was identified by amino acid sequence similarity to plant andmammalian ankyrin-repeat proteins. G1950 is found in the sequence of thechromosome 2 BAC T4M8 (GenBank accession number AC006284.3;nid=6598551), released by the Arabidopsis Genome Initiative. G1950 hasno distinctive features other than the presence of a 33-aa repeatedankyrin element known for protein-protein interaction, in the C-terminusof the predicted protein Amino acid sequence comparison shows similarityto Arabidopsis NPR1.

Experimental Observations

The 5′ and 3′ ends of G1950 were experimentally determined by RACE, andfound to correspond to the prediction in GenBank. The analysis of theendogenous expression level of G1950, as determined by RT-PCR, revealedthat expression was specific to embryos, siliques and germinating seeds(young seedlings). G1950 expression was induced upon auxin treatment,which indicated that G1950 plays an important role in seed/embryodevelopment or other processes specific to seeds (stress-related ordesiccation-related).

The function of G1950 was analyzed in transgenic plants overexpressingG1950 under the control of the 35S promoter. When compared to wild-typecontrols, plants overexpressing G1950 were more tolerant to infectionwith the necrotrophic fungal pathogen Botrytis cinerea. The experimentwas confirmed using mixed and individual lines. This result indicatedthat G1950, an Arabidopsis ankyrin protein with similarity to NPR1, mayplay a similar role to NPR1 in disease pathways. Transformants weremorphologically indistinguishable from wild-type plants, and showed nobiochemical changes in comparison to controls.

Potential Applications

35S::G1950 overexpression in Arabidopsis or ectopic expression in leaveshas been shown to affect the onset of disease following inoculation withBotrytis cinerea. Therefore, G1950 or its equivalogs could be used tomanipulate the defense response in order to generate pathogen-resistantplants. Furthermore, seed or embryo-specific expression of G1950 mayindicate a potential function for this gene or its equivalogs in seeddevelopment. The G1950 promoter could be useful for targeted geneexpression in seeds.

G1958 (SEQ ID NO: 383)

Published Information

G1958 was identified in the sequence of BAC T5F17, GenBank accessionnumber AL049917, released by the Arabidopsis Genome Initiative.

G1958 has also been published as PHR1. Mutants in PHR1 show reducedgrowth under conditions of phosphate starvation and fail to induce genesnormally regulated by low phosphate concentration (Rubio et al. (2001)Genes Devel. 15: 2122-2133).

Closely Related Genes from Other Species

G1958 is a member of a subclass of GARP family members that contains asecond conserved domain with a somewhat regularly spaced pattern ofglutamine residues. Members of this subclass are apparent in many otherplant species. A potential ortholog of G1958 is a putative transcriptionfactor from tobacco, WREBP-1 (accession number BAA75684). G1958 is theclosest Arabidopsis relative of this tobacco gene, and similaritybetween G1958 and WREBP-1 extends beyond the signature motifs of thefamily to a level that would suggest the genes are orthologous.Therefore, WREBP-1 may have a function and/or utility similar to that ofG1958.

Experimental Observations

The full-length coding sequence of G1958 was determined G1958 was foundto be expressed throughout the plant with highest expression in rosetteleaves, flowers, and embryos, and was induced by auxin and heat. A linehomozygous for a T-DNA insertion in G1958 was used to determine thefunction of this gene. The T-DNA insertion of G1958 was approximately90% into the coding sequence of the gene, within a region of amino acidsequence conservation that seems to define a subfamily of GARP proteins,and therefore is likely to result in a null mutation. The phenotype ofthese knockout plants was wild-type in all assays performed, except thatthey were smaller and showed less root growth than control plants whengrown on plates. This phenotype may have been environmentally influencedas it was accentuated when seedlings were transferred to stressconditions, while in contrast, small size was not noted in thesoil-grown plants. G1958 was apparently necessary for optimum growth anddevelopment. However, the subtle phenotype indicated that G1958 could bepartially redundant. G1958 is a member of a small GARP subfamily withhigh sequence similarity, so it is possible that other homologs mighthave overlapping function.

G1958 knockout mutants had increased seed oil and decreased seed proteincontent compared to wild-type plants.

Potential Applications

G1958 or its equivalogs may be used to alter seed protein content, whichmay be very important for the nutritional value and production ofvarious food products

G1958 or its equivalogs could also be used to manipulate plant growth,in particular root growth.

G2007 (SEQ ID NO: 385)

Published Information

G2007 belongs to the MYB-(R1)R2R3 family of transcription factors. G2007corresponds to the previously described gene Myb42 (Stracke andWeisshaar, 1999; direct submission of the sequence to GenBank).

Closely Related Genes from Other Species

A myb gene from Pimpinella brachycarpa (AAF22256) is related to G2007.Similarity between G2007 and this Pimpinella myb extends beyond thesignature motif of the family to a level that would suggest the genesare orthologous.

Experimental Observations

The complete sequence of G2007 was determined. The function of this genewas analyzed using transgenic plants in which G2007 was expressed underthe control of the 35S promoter. The phenotype of these transgenicplants was wild-type in all biochemical and physiological assaysperformed. However, overexpression of G2007 resulted in a delayed switchto flowering. Under continuous light conditions, 35S::G2007 plantsproduced approximately twice as many primary rosette leaves as controls,and formed flower buds up to two weeks late. As a consequence of thisdelay in flowering, the plants also senesced later than wild type. Allthe plants from two independent T2 lines exhibited this phenotype, inboth an initial and a repeat planting. Late flowering was also notedamongst some of the primary transformants, but the T1 generation showedconsiderable morphological variation, making this trait more difficultto discern.

G2007 appeared to be constitutively expressed at moderate levels in alltissues tested except germinating seeds where no expression wasdetected. There was no induction of G2007 in response to anyenvironmental condition tested.

Potential Applications

G2007 or its equivalogs may be used to delay flowering in plants. Inspecies such as sugarbeet where the vegetative parts of the plantsconstitute the crop and the reproductive tissues are discarded, it wouldbe advantageous to delay or prevent flowering. Extending vegetativedevelopment could bring about large increases in yields.

Additionally, a major concern is the escape of transgenic pollen fromGMOs to wild species or so-called organic crops. Genes such as G2007 orits equivalogs that prevent vegetative transgenic crops from floweringwould eliminate this worry.

G2010 (SEQ ID NO: 387)

Published Information

G2010 is a member of the SBP family of transcription factors andcorresponds to spl4 (Cardon et al., 1999). Expression of spl4 isup-regulated during development under both long day and short dayconditions and is highly expressed in the inflorescence tissue.Expression of G2010 is localized to the rib meristem andinter-primordial regions of the inflorescence apex (Cardon et al (1999)Gene 237:91-104).

Closely Related Genes from Other Species

A gene related to G2010 is squamosa-promoter binding protein 1 fromAntirrhinum majus.

Experimental Observations

The complete sequence of G2010 was determined. The function of this genewas analyzed using transgenic plants in which G2010 was expressed underthe control of the 35S promoter. Overexpression of G2010 resulted in aclear reduction in time to flowering. Under continuous light conditions,at 20-25° C., three independent T2 lines of 35S::G2010 plants floweredapproximately one week earlier than wild-type controls. The primaryshoot of 35S::G2010 plants switched to reproductive growth afterproducing 5-6 rosette leaves, compared with 8-10 rosette leaves incontrols. Flower buds were first visible 12-14 days after sowing in35S::G2010 plants compared with approximately 20 days for wild type.35S::G2010 transformants were also observed to begin senescence soonerthan controls. Otherwise, plants overexpressing G2010 are wild-type inphenotype.

Expression of G2010 was not detected by RT-PCR in any of the tissuestested. G2010 was slightly induced in rosette leaves in response to heatand cold stress treatments as well as salicylic acid treatment. Theexpression profile for G2010 indicated that this gene is involved in aplant's transition to flowering normally and in response to stressfulenvironmental conditions.

Potential Applications

The potential utility of a gene such as G2010 or its equivalogs is toaccelerate flowering time.

G2053 (SEQ ID NO: 389)

Published Information

G2053 was identified in the sequence of BAC T27C4, GenBank accessionnumber AC022287, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G2053 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G2053 in Arabidopsis resulted in plants with altered osmotic stresstolerance. In a root growth assay on media containing highconcentrations of PEG, G2053 overexpressors showed more root growthcompared to wild-type controls.

Potential Applications

Based on the altered stress tolerance induced by G2053 overexpression,this transcription factor or its equivalogs could be used to alter aplant's response water deficit conditions and, therefore, could be usedto engineer plants with enhanced tolerance to drought, salt stress, andfreezing.

G2059 (SEQ ID NO: 391)

Published Information

G2059 corresponds to gene AT4g13620 (CAB78404).

Experimental Observations

The function of G2059 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G2059plants did not show major alterations in morphology and development, andwere wild-type in the physiological and biochemical analyses that wereperformed. However, subtle changes in rosette leaf morphology weredetected in 35S::G2059 transformants: rosette leaves were slightlydarker green and rather narrow compared to controls.

G2059 expression was detected by RT-PCR in root tissue, and it appearedto be ectopically induced by heat stress.

As measured by NIR, G2059 overexpressors had decreased seed oil contentand increased seed protein content compared to wild-type plants.

Potential Applications

G2059 or equivalog overexpression may be used to alter seed oil content,which may be very important for the nutritional value and production ofvarious food products

G2085 (SEQ ID NO: 393)

Published Information

G2085 was identified as a gene in the sequence of BAC AL078637, releasedby the Arabidopsis Genome Initiative.

Experimental Observations

The sequence of G2085 was experimentally determined and the datapresented for this gene were from plants homozygous for a T-DNAinsertion in G2085. The T-DNA insertion of this gene was found to be incoding sequence and therefore this knockout mutant was likely to containa null allele of G2085.

Although G2085 was constitutively expressed throughout the plant, itsexpression was markedly repressed by a variety of stress conditions suchas ABA, cold, osmotic stress and Erysiphe, indicating that it may be anegative regulator of stress responses in Arabidopsis.

Seed of these G2085 knockout mutants had increased size and alteredcolor.

Potential Applications

G2085 or its equivalogs could be used to modify seed size and/ormorphology, which could have an impact on yield and appearance

G2105 (SEQ ID NO: 395)

Published Information

G2105 was discovered as a gene in BAC T22K18, accession number AC010927,released by the Arabidopsis genome initiative.

Closely Related Genes from Other Species

G2105 has similarity within the conserved domain of non-Arabidopsisproteins.

Experimental Observations

The ORF boundary of G2105 (SEQ ID NO: 395) was determined and G2105 wasanalyzed using transgenic plants in which G2105 was expressed under thecontrol of the 35S promoter. Two of four T2 lines examined appeared darkgreen and were smaller than wild type at all stages of development.Additionally, the adaxial leaf surfaces from these plants had a somewhat‘lumpy’ appearance caused by trichomes being raised-up on small moundsof epidermal cells. Two lines of G2105 overexpressing plants had largerseed. G2105 expression was root specific and induced in leaves by auxin,abscisic acid, high temperature, salt and osmotic stress treatments.

Potential Applications

On the basis of the analyses, G2105 or its equivalogs can be used tomanipulate some aspect of plant growth or development, particularly intrichome development.

In addition, G2105 or its equivalogs can be used to modify seed sizeand/or morphology, which can have an impact on yield.

The promoter of G2105 can have some utility as a root specific promoter.

G2110 (SEQ ID NO: 397)

Published Information

G2110 corresponds to gene F6A14.5 (AAF27095).

Closely Related Genes from Other Species

G2110 shows sequence similarity, outside of the conserved WRKY domain,with other proteins of the family from several plant species, such asAC007789_(—)9 putative WRKY DNA binding protein (gi5042446), from rice.

Experimental Observations

The function of G2110 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G2110 resulted in plants with an altered salt stress response. In aroot growth assay on media containing high concentrations of NaCl, G2110overexpressing lines showed more seedling vigor, less bleaching and moreroot growth compared to wild-type control plants. In repeat experiments,all three lines showed the salt tolerance phenotype.

No consistent alterations in plant morphology resulted from G2110overexpression, and 35S::G2110 plants were wild-type in the biochemicalanalyses that were performed.

G2110 was expressed in a variety of tissues, including flower, shoot,embryo, silique, and germinating seedling samples; its expression wasnot detected in leaf and root tissues. G2110 expression may have beenaltered by several physiological conditions, and, was ectopicallyinduced by auxin and by heat.

Potential Applications

G2110 or its equivalogs could be used to improve plant performance underconditions of salt stress. Evaporation from the soil surface causesupward water movement and salt accumulation in the upper soil layerwhere the seeds are placed. Thus, germination normally takes place at asalt concentration that is higher than the mean salt concentration inthe whole soil profile. Increased salt tolerance during the germinationstage of a crop plant would impact survivability and yield.

G2114 (SEQ ID NO: 399)

Published Information

G2114 corresponds to gene F28P22.24 (AAF21171).

Closely Related Genes from Other Species

G2114 shows sequence similarity, outside of the conserved AP2 domain,with other proteins of the family from several plant species, such asthe one from Glycine max represented by EST AW780688 s175e07.y1 Gm-c1027Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1027-7165.

Experimental Observations

The function of G2114 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Seeds from oneof the T2 populations were larger than controls. This effect wasapparent in seed from this population's primary transformant, but wasnot noted in the other T2 populations.

35S::G2114 plants were wild-type in the physiological and biochemicalanalyses that were performed.

G2114 expression was primarily detected in embryo, silique, andgerminating seedling tissue. G2114 was not ectopically induced by any ofthe biotic and abiotic stress conditions tested.

Potential Applications

G2114 or its equivalogs could be used to modify seed size and/ormorphology, which could have an impact on yield and appearance

G2117 (SEQ ID NO: 401)

Published Information

G2117 was identified in the sequence of BAC T6L1, GenBank accessionnumber AC011665, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G2117 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter.

Plants overexpressing G2117 had altered leaf morphology, coloration, andsmaller overall plant size and were generally small with short, rounded,dark green leaves that became curled later in development. These plantsgenerated thin inflorescence stems developed a rather bushy appearance,and had reduced fertility. Overexpression of G2117 in Arabidopsis alsoresulted in an increase in seed glucosinolate M39497 in two T2 lines. Noother phenotypic alterations were observed.

G2117 appeared to be highly expressed in roots compared to all othertissues tested.

G2117 overexpressors had increased seed protein content compared towild-type plants.

Potential Applications

G2117 or its equivalogs may be useful for altering seed glucosinolatecomposition.

G2117 or equivalog overexpression may also be used to alter seed proteincontent, which may be very important for the nutritional value andproduction of various food products.

G2123 (SEQ ID NO: 403)

Published Information

G2123 corresponds to a predicted putative 14-3-3 protein in annotatedBAC clone T11I11 (AC012680), from chromosome 1 of Arabidopsis.

Closely Related Genes from Other Species

Because there is a high degree of similarity among all GF14 proteins,there are several GF14 proteins from other plant species, which areclosely related to G2123.

Experimental Observations

G2123 corresponds to a predicted putative 14-3-3 protein in annotatedBAC clone T11I11 (AC012680), from chromosome 1 of Arabidopsis.

G2123 (SEQ ID NO: 403) was analyzed using transgenic plants in whichG2123 was expressed under the control of the 35S promoter. The phenotypeof these transgenic plants was wild-type in all assays performed. G2123was expressed primarily in developing seeds and silique tissue inwild-type plants.

G2123 overexpressors produced more seed oil than wild-type plants.

Potential Applications

G2123 or its equivalogs could used to increase seed oil content, whichwould be of value for modifying the nutritional value and caloriccontent of food for human consumption as well as animal feeds, and maybe of value in improving seed storage characteristics.

G2130 (SEQ ID NO: 405)

Published Information

G2130 was identified in the sequence of BAC clone F15G16 (AL132959, geneF15G16.20).

Closely Related Genes from Other Species

G2130 shows sequence similarity, outside of the conserved AP2 domain,with a protein from Medicago truncatula, represented by EST sequenceAW685524.

Experimental Observations

The function of G2130 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G2130plants showed a variety of morphological and physiological alterations.Overexpression of G2130 reduced overall plant size, resulted inpremature senescence, and compromised fertility. 35S::G2130 plants weresmaller than wild-type controls throughout development. At around thetime of bolting, leaves developed yellow patches of senesced tissue. Theinflorescences from these plants were generally very thin and carriedflowers with poorly developed stamens. Many flowers senesced withoutpollination and failed to develop a silique.

G2130 overexpressing lines showed more seedling vigor in a heat stresstolerance germination assay compared to wild-type controls. However, nodifference was detected in the heat stress response assay, which isperformed on older seedlings, indicating that the phenotype could bespecific for germination. G2130 overexpressing lines are also somewhatmore sensitive to chilling: the plants are more chlorotic and stuntedwhen grown at 8° C. compared to the wild-type controls. They also showedmore disease symptoms following inoculation with a low dose of thefungal pathogen Botrytis cinerea.

G2130 was ubiquitously expressed and did not appear to be significantlyinduced by any of the conditions tested.

Potential Applications

G2130 or its equivalogs could be used to improve seed germination underheat stress.

G2133 (SEQ ID NO: 407)

Published Information

G2133 corresponds to gene F26A9.11 (AAF23336).

Closely Related Genes from Other Species

G2133 shows sequence similarity with known genes from other plantspecies within the conserved AP2/EREBP domain.

Experimental Observations

G2133 (SEQ ID NO: 407) was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G2133 caused a variety of alterations in plant growth anddevelopment: delayed flowering, altered inflorescence architecture, anda decrease in overall size and fertility. At early stages, 35S::G2133transformants were markedly smaller than controls and displayed curled,dark-green leaves. Most of these plants remained in a vegetative phaseof development substantially longer than controls, and produced anincreased number of leaves before bolting. In the most severely affectedplants, bolting occurred more than a month later than in wild type(24-hour light). In addition, the plants displayed a reduction in apicaldominance and formed large numbers of shoots simultaneously, from theaxils of rosette leaves. These inflorescence stems had short internodes,and carried increased numbers of cauline leaf nodes, giving them a veryleafy appearance. The fertility of 35S::G2133 plants was generally verylow. In addition, G2133 overexpressing lines were more resistant to theherbicide glyphosate. In a repeat experiment, two lines were moretolerant while one line was wild type. G2133 expression was detected ina variety of tissues: flower, leaf, embryo, and silique samples. Itsexpression was altered by several conditions, including auxin treatment,osmotic stress, and Fusarium infection.

Potential Applications

G2133 or its equivalogs can be used for the generation of glyphosateresistant plants, and to increase plant resistance to oxidative stress.

G2133 or its equivalogs may also be used to delay flowering in plants.

G2138 (SEQ ID NO: 409)

Published Information

G2138 corresponds to gene F23N20.12 (AAF26022).

Experimental Observations

The function of G2138 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G2138plants were wild-type in morphology and development. G2138overexpressors produced more seed oil than wild-type plants.

Potential Applications

G2138 or its equivalogs could used to increase seed oil content, whichwould be of value for modifying the nutritional value and caloriccontent of food for human consumption as well as animal feeds, and maybe of value in improving seed storage characteristics.

G2140 (SEQ ID NO: 411)

Published Information

The sequence of G2140 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AC011665, based on its sequencesimilarity within the conserved domain to other bHLH related proteins inArabidopsis. G2140 corresponds to gene F14K14.8 (AAG52041).

Closely Related Genes from Other Species

G2140 proteins show extensive sequence similarity with a tomato ovarycDNA, TAMU Lycopersicon esculentum (AI488313) and a Glycine max cDNAclone (BE020519).

Experimental Observations

The complete sequence of G2140 (SEQ ID NO: 411) was determined G2140 wasexpressed throughout the plant. It showed repression by salicylic acidand Erysiphe infection. Overexpressing G2140 in Arabidopsis resulted inseedlings that were more tolerant to osmotic stress conditions. Ingermination assays where seedlings were exposed to high concentrationsof sucrose or NaCl, all three lines tested showed better cotyledonexpansion and seedling vigor. Additionally, G2140 overexpressing plantsshowed insensitivity to ABA in a germination assay. In general, G2140overexpressing plants were small and sickly with short roots when grownin Petri plates. The combination of ABA insensitivity and resistance toosmotic stress at germination had also been observed for other genes,for example, G1820 and G926. Significantly, the ABA resistance wasdetected in a germination assay. ABA is involved in maintaining seeddormancy, and it is possible that ABA insensitivity at the germinationstage promotes germination despite unfavorable conditions.

When grown in soil, G2140 overexpressing plants displayed marked changesin Arabidopsis leaf and root morphology. All twenty of the 35S::G2140primary transformants displayed, to various extents, leaves withupcurled margins. In the most severe cases, the leaves became highlycontorted and the plants were slightly small and grew more slowly thancontrols. Three T1 lines that showed substantial levels of G2140overexpression (determined by RT-PCR) were chosen for further study. TheT2 seedlings from each of these lines exhibited stunted roots comparedwith controls. Seedlings from two of these lines also showed upcurledcotyledons. At later stages, however, some T2-plants appeared wild type.Plants from some T2-populations were rather varied in size and showedhints of leaf curling later in development. However, this effect wasless severe than that seen in the T1 lines. To verify the leaf-curlingphenotype, two further T2 populations were morphologically examined;seedlings from one line were found to be extremely tiny with thickenedhypocotyls and short stunted roots. Such plants were too small fortransfer to soil. However, another line of T2-18 showed slightlycontorted cotyledons and formed severely upcurled leaves, confirming theeffects seen in the T1 generation.

Potential Applications

G2140 affects ABA sensitivity, and thus when transformed into a plantthis transcription factor or its equivalogs may diminish cold, drought,oxidative and other stress sensitivities, and also be used to alterplant architecture, and yield.

G2140 or its equivalogs are useful for creating plants that germinatebetter under conditions of high salt. Evaporation from the soil surfacecauses upward water movement and salt accumulation in the upper soillayer where the seeds are placed. Thus, germination normally takes placeat a salt concentration much higher than the mean salt concentration inthe whole soil profile. Increased salt tolerance during the germinationstage of a crop plant will impact survivability and yield. In addition,G2140 or its equivalogs can be used to alter a plant's response to waterdeficit conditions and, therefore, can be used to engineer plants withenhanced tolerance to drought, and freezing.

G2143 (SEQ ID NO: 413)

Published Information

The sequence of G2143 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AL132976, based on its sequencesimilarity within the conserved domain to other bHLH related proteins inArabidopsis. G2143 corresponds to gene F11C1_(—)170 (CAB62312).

Closely Related Genes from Other Species

G2143 protein shared extensive homology in the basic helix loop helixregion with a protein encoded by Glycine max cDNA clones (AW832545,BG726819 and BG154493) and a Lycopersicon esculentum cDNA clone(BE451174). There was lower homology outside of the region.

Experimental Observations

G2143 (SEQ ID NO: 413) is a member of a clade of highly related HLH/MYCproteins that also includes G779, G1063, G1499, and G2557. All of thesegenes caused similar pleiotropic phenotypic effects when overexpressed,the most striking of which was the production of ectopic carpelloidtissue. These genes can be considered key regulators of carpeldevelopment. Twelve out of twenty 35S::G2143 T1 lines showed a verysevere phenotype; these plants were markedly small and had narrow,curled, dark-green leaves. Such individuals were completely sterile andformed highly abnormal inflorescences; shoots often terminated inpin-like structures, and flowers were replaced by filamentous carpelloidstructures, or a fused mass of carpelloid tissue. Furthermore, lateralbranches usually failed to develop, and tiny patches of stigmatic tissueoften formed at axillary nodes of the inflorescence. Strongly affectedplants displayed the highest levels of transgene expression (determinedby RT-PCR). The remaining T1 lines showed lower levels of G2143overexpression; these plants were still distinctly smaller than wildtype, but had relatively normal inflorescences and produced seed. Sincethe strongest 35S::G2143 lines were sterile, three lines with arelatively weak phenotype, that had produced sufficient seed forbiochemical analysis, were selected for further study. T2-11 plantsdisplayed a very mild phenotype and had somewhat small, narrow, darkgreen leaves. The other two T2 populations, however, appeared wild type,suggesting that transgene activity might have been reduced between thegenerations. Reduced seedling vigor was noted in the physiologicalassays. G2143 expression was detected at low levels in flowers andsiliques, and at higher levels in germinating seed.

Potential Applications

G2143 or its equivalogs can be used to manipulate flower form andstructure or plant fertility. One application for manipulation of flowerstructure can be in the production of saffron, which is derived from thestigmas of Crocus sativus.

G2144 (SEQ ID NO: 415)

Published Information

The sequence of G2144 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AL132977, based on its sequencesimilarity within the conserved domain to other bHLH related proteins inArabidopsis. G2144 corresponds to gene T10K17.10 (CAB67608).

Experimental Observations

The complete sequence of G2144 was determined. G2144 was expressed atlow to moderate levels throughout the plant. It was not significantlyinduced or repressed by any of the conditions tested.

The function of this gene was analyzed using transgenic plants in whichG2144 was expressed under the control of the 35S promoter.Overexpression of G2144 in Arabidopsis produced pleiotropicmorphological changes that indicate the gene might affect lightregulated development, or shade avoidance responses. At the seedlingstage, 35S::G2144 transformants often exhibited elongated cotyledons andhypocotyls. Later, the plants developed rather pale, narrow, flat leavesthat had long petioles, and were sometimes positioned in a verticalorientation. Flowering occurred several days earlier than in wild typeand inflorescence stems were typically rather thin and spindly.Interestingly, in many of the plants, inflorescence stems sporadicallysplit open at later stages. Additionally, in some plants, large numbersof secondary leaves developed in the axils of primary rosette leaves,and occasionally, internode elongation occurred between rosette leaves.It should also be noted that fertility was often poor; flowers sometimesfailed to properly open or showed contorted organs, and seed yield waslow.

Morphological alterations in the 35S::G2144 plants were somewhat similarto those in the 35S::G1494 plants.

In addition, overexpression of G2144 in Arabidopsis resulted in anincrease in leaf glucosinolate M39480 in two T2 lines.

Potential Applications

G2144 or its equivalogs can be used to alter how plants respond tolight. For example, it may be used to manipulate plant growth anddevelopment, and flowering time.

G2144 or its equivalogs could be used to alter glucosinolate compositionin plants.

G2144 or its equivalogs could also be used to alter flowering time.

G2153 (SEQ ID NO: 417)

Published Information

The sequence of G2153 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AC011437, based on its sequencesimilarity within the conserved domain to other AT-hook related proteinsin Arabidopsis. G2153 corresponds to gene F7O18.4 (AAF04888).

Closely Related Genes from Other Species

G2153 protein shows extensive sequence similarity with Oryza sativachromosome 2 and 8 clones (AP004020 and AP003891), a Lotus japonicuscDNA (AW720668) and a Medicago truncatula cDNA clone (AW574000).

Experimental Observations

The complete sequence of G2153 was determined. G2153 was stronglyexpressed in roots, embryos, siliques, and germinating seed, but at lowor undetectable levels in shoots, flowers, and rosette leaves. It wasnot significantly induced or repressed by any condition tested.

The function of this gene was analyzed using transgenic plants in whichG2153 was expressed under the control of the 35S promoter.Overexpression of G2153 in Arabidopsis resulted in seedlings with analtered response to osmotic stress. In a germination assay on mediacontaining high sucrose, G2153 overexpressors had more expandedcotyledons and longer roots than the wild-type controls. This phenotypewas confirmed in repeat experiments on individual lines, and all threelines showed osmotic tolerance. Increased tolerance to high sucrosecould also be indicative of effects on sugar sensing. Overexpression ofG2153 produced no consistent effects on Arabidopsis morphology, and noaltered phenotypes were noted in any of the biochemical assays.

Potential Applications

G2153 or its equivalogs can be used to alter a plant's response to waterdeficit conditions and, therefore, could be used to engineer plants withenhanced tolerance to drought, salt stress, and freezing.

G2153 or its equivalogs may also be useful for altering a plant'sresponse to sugars.

G2155 (SEQ ID NO: 419)

Published Information

The sequence of G2155 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AC012188, based on its sequencesimilarity within the conserved domain to other AT-hook related proteinsin Arabidopsis.

Closely Related Genes from Other Species

G2155 protein shows extensive sequence similarity with Medicagotruncatula cDNA clones (BG646893 and BG647027) and a Glycine max cDNAclone (BI426899).

Experimental Observations

The complete sequence of G2155 was determined. G2155 expression wasdetected at low levels only in flowers and embryos. It was not inducedin rosette leaves by any condition tested.

The function of this gene was analyzed using transgenic plants in whichG2155 was expressed under the control of the 35S promoter.Overexpression of G2155 produced a marked delay in the time toflowering. Under continuous light conditions, 35S::G2155 transformantsdisplayed a considerable extension of vegetative development, andtypically formed flower buds about two weeks later than wild-typecontrols. At early stages, the plants were slightly small and had ratherrounded leaves compared to wild type. However, later in development,when the leaves were fully expanded, 35S::G2155 plants became verylarge, dark-green, and senesced much later than controls.

In addition, overexpression of G2155 resulted in an increase in seedglucosinolate M39497 in two T2 lines. No other phenotypic alterationswere observed in any of the biochemical or physiological assays.

Potential Applications

G2155 or equivalog overexpression may be used to delay flowering.

G2155 or its equivalogs could also be used to alter seed glucosinolatecomposition.

G2192 (SEQ ID NO: 421)

Published Information

G2192 was identified in the sequence of BAC T19F6, GenBank accessionnumber AC002343, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G2192 is very similar to a rice gene on clone P0708G2, accession numberAP001539, released as part of the rice genome sequencing project.Homology between G2192 and this rice gene extends well beyond theconserved domain and thus the two genes may be orthologs.

Experimental Observations

The annotation of G2192 in BAC AC002343 was experimentally determined.The function of this gene was analyzed using transgenic plants in whichG2192 was expressed under the control of the 35S promoter.Overexpression of G2192 in Arabidopsis resulted in an decrease in 18:3fatty acid in seeds in two T2 lines. These lines also showed changes in16:0, 18:0 and 18:2 fatty acids.

G2192 appeared to be constitutively expressed in all tissues andenvironmental conditions tested.

Potential Applications

G2192 or its equivalogs may have utility to alter seed fatty acidcomposition, which would be of significant nutritional value.

G2295 (SEQ ID NO: 423)

Published Information

G2295 corresponds to gene K19M22.9 (BAB09634).

Experimental Observations

The function of G2295 was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G2295 accelerated flowering by up to one week under 24-hour lightconditions. Early flowering was apparent in all plants from twoindependent 35S::G2295 T2 lines in each of two separate sowings.Additionally, these plants had rather flat leaves compared to wild type.In the T1 generation, five of twenty lines also flowered markedlyearlier than controls.

According to the results obtained in the RT-PCR experiments, G2295 wasspecifically expressed in embryo and silique tissues. It was not clearwhether the alterations in flowering time observed in the 35S::G2295overexpressors reflected the true function of the gene. There havealready been cases described of Arabidopsis transcription factor genesthat are specifically expressed in flower-derived tissues but that canaffect flowering time when their expression pattern is modified,including a homeobox gene long considered representing a true floweringtime locus, FWA Similar examples have been found (e.g., G183).

35S::G2295 plants were wild-type in the physiological and biochemicalanalyses that were performed.

Potential Applications

G2295 or its equivalogs could be used to modify flowering timecharacteristics. In addition, the promoter of the gene could be used todrive embryo/silique-specific gene expression.

G2340 (SEQ ID NO: 425)

Published Information

G2340 is a member of the (R1)R2R3 subfamily of MYB transcriptionfactors. G2340 corresponds to gene At1g74080 (AAK54746), and is alsoreferred to as MYB122.

Closely Related Genes from Other Species

G2340 shows sequence similarity with known genes from other plantspecies within the conserved Myb domain.

Experimental Observations

G2340 (SEQ ID NO: 425) was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G2340 produced a spectrum of deleterious effects on Arabidopsisgrowth and development. 35S::G2340 primary transformants were generallysmaller than controls, and at early stages some displayed leaves thatwere held in a vertical orientation. The most severely affected linesdied at early stages. Others survived, but displayed necrosis of theblades in later rosette leaves and cauline leaves. Inflorescencedevelopment was also highly abnormal; stems were typically shorter thanwild type, often ‘kinked’ at nodes, and the tissue had a rather fleshysucculent appearance. Flower buds were frequently poorly formed, failedto open and withered away without siliques developing. Additionally,secondary shoot growth frequently failed the tips of such structuressometimes senesced. Due to these abnormalities, many of the primarytransformants were completely infertile. Three T1 lines with arelatively weak phenotype, which did set some seed, were selected forfurther study. Plants from one T2-population displayed a strongphenotype, and died early in development. The other two T2 populationswere slightly small, but the effects were much weaker than those seen inthe parental plants, suggesting that activity of the transgene mighthave become reduced between the generations. It should be noted thatG2340 and G671 are part of the same clade and that they had very similarmorphological phenotypes and a similar expression pattern. These twogenes may have overlapping or redundant phenotypes in the plant. Small,pale seedlings with strap-like leaves that held a vertical orientationwere found in the mixed line populations of 35S::G2340 transgenicseedlings when grown under sterile conditions, similar to those observedin soil grown plants in the T1 generation. The necrotic lesions observedon the T1 plants grown in soil were not observed on the plants grown inculture. The necrotic lesion phenotype is a classic lesion mimicphenotype and would suggest that G2340 is involved in cell deathresponses, or alternatively G2340 overexpressor plants arehypersensitive to stresses. One class of lesion mimic forms progressivelesions following an inductive stress. Lesion formation may be inducedin G2340 overexpressing plants grown in culture. In addition to themorphological changes, overexpression of G2340 resulted in an extremealteration in seed glucosinolate profile. This phenotype was observed inone line in seed from two independent plantings. According to RT-PCRanalysis, G2340 was expressed primarily in roots and was slightlyinduced in leaf tissue in response to auxin and heat treatments.

Potential Applications

G2340 or its equivalogs can be used to engineer plants with an induciblecell death response. A gene that regulates cell death in plants can beused to induce a pathogen protective hyper-response (HR) in plantswithout the potentially detrimental consequences of a constitutivesystemic acquired resistance (SAR). Other potential utilities includethe creation of novel abscission zones or inducing death in reproductiveorgans to prevent the spread of pollen, transgenic or otherwise. In thecase of necrotrophic pathogens that rely on dead plant tissue as asource of nutrients, prevention of cell death could confer tolerance tothese diseases. Overexpression of G2340 in Arabidopsis also resulted inan extreme alteration in seed glucosinolate profile. Therefore, the geneor its equivalogs can be used to alter glucosinolate composition inplants.

G2343 (SEQ ID NO: 427)

Published Information

G2343 is a member of the R2-R3 subfamily of Myb transcription factors.The gene was identified as part of BAC T12P18, accession numberAC010852, released by the Arabidopsis Genome Initiative. A cDNA sequencecorresponding to G2343 was submitted to GenBank, accession numberAF214116, with the gene name MYB103.

Closely Related Genes from Other Species

The most related gene to G2343 is tomato gene LETHM1 (CAA64615)Similarity between G2343 and LETHM1 extends beyond the signature motifof the family to a level that would suggest the genes are orthologs.

Experimental Observations

The complete sequence of G2343 (SEQ ID NO: 427) was determined and G2343was analyzed using transgenic plants in which G2343 was expressed underthe control of the 35S promoter. The phenotype of these transgenicplants was wild-type in all assays performed. As determined by RT-PCR,G2343 was expressed in shoots, embryos and siliques. G2343 expressionwas induced in rosette leaves by auxin, heat stress, and infection byFusarium oxysporum.

As measured by NIR, G2343 overexpressors had altered seed oil contentcompared to wild-type plants.

Potential Applications

G2343 or equivalog overexpression may be used to alter seed oil content,which may be very important for the nutritional value and production ofvarious food products

G2346 (SEQ ID NO: 429)

Published Information

G2346 was identified in the sequence of BAC clone T10K17, GenBankaccession number AL132977, released by the Arabidopsis Genome Initiative

Closely Related Genes from Other Species

G2346 shows sequence similarity with known genes from other plantspecies within the conserved SBP domain.

Experimental Observations

G2346 (SEQ ID NO: 429) was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G2346seedlings from all three T2 populations had larger cotyledons and weremore advanced than controls. This indicated that the seedlings developedmore rapidly that the control plants. At later stages, however, G2346overexpressing plants showed no consistent differences from controlplants. The phenotype of these transgenic plants was wild-type in allother assays performed. According to RT-PCR analysis, G2346 wasexpressed ubiquitously.

Potential Applications

G2346 or its equivalogs can be used to produce plants that develop morequickly, particularly at early stages. For almost all commercial crops,it is desirable to use plants that establish more quickly, sinceseedlings and young plants are particularly susceptible to stressconditions such as salinity or disease. Since many weeds may outgrowyoung crops or out-compete them for nutrients, it would also bedesirable to determine means for allowing young crop plants to outcompete weed species. Increasing seedling growth rate (emergence)contributes to seedling vigor and allows for crops to be planted earlierin the season with less concern for losses due to environmental factors.Early planting helps add days to the critical grain-filling period andincreases yield.

G2347 (SEQ ID NO: 431)

Published Information

G2347 is a member of the SBP family of transcription factors andcorresponds to spl5 (Cardon et al., 1999). Expression of spl5 isup-regulated in seedlings during development under both long day andshort day conditions and is highly expressed in the inflorescencetissue. Expression of G2347 is specifically localized in theinflorescence apical meristem and young flowers (Cardon et al. (1999)Gene 237: 91-104).

Closely Related Genes from Other Species

The closest relative to G2347 is the Antirrhinum protein, SBP2(CAA63061). The similarity between these two proteins is extensiveenough to suggest they might have similar functions in a plant.

Experimental Observations

G2347 (SEQ ID NO: 431) was analyzed using transgenic plants in whichG2347 was expressed under the control of the 35S promoter.Overexpression of G2347 markedly reduced the time to flowering inArabidopsis. This phenotype was apparent in the majority of primarytransformants and in all plants from two out of the three T2 linesexamined. Under continuous light conditions, 35S::G2347 plants formedflower buds up a week earlier than wild type. Many of the plants wererather small and spindly compared to controls. To demonstrate thatoverexpression of G2347 could induce flowering under less inductivephotoperiods, two T2 lines were re-grown in 12 hour conditions; again,all plants from both lines bolted early, with some initiating flowerbuds up to two weeks sooner than wild type. As determined by RT-PCR,G2347 was highly expressed in rosette leaves and flowers, and to muchlower levels in embryos and siliques. No expression of G2347 wasdetected in the other tissues tested. G2347 expression was repressed bycold, and by auxin treatments and by infection by Erysiphe. G2347 isalso highly similar to the Arabidopsis protein G2010. The level ofhomology between these two proteins suggested they could have similar,overlapping, or redundant functions in Arabidopsis. In support of thishypothesis, overexpression of both G2010 and G2347 resulted in earlyflowering phenotypes in transgenic plants.

Potential Applications

G2347 or its equivalogs may be used to modify the time to flowering inplants.

G2379 (SEQ ID NO: 433)

Published Information

G2379 was identified in the sequence of BAC MOP10, GenBank accessionnumber AB005241, released by the Arabidopsis Genome Initiative.

Experimental Observations

The annotation of G2379 in BAC AB005241 was experimentally confirmed.The function of this gene was analyzed using transgenic plants in whichG2379 was expressed under the control of the 35S promoter. G2379overexpressing plants showed increased seedling vigor when grown onmedia containing elevated sucrose levels. This phenotype might beindicative of either altered sugar sensing or increased tolerance ofosmotic stress. No altered morphological or biochemical phenotypes wereobserved. G2379 appeared to be constitutively expressed in all tissuesand environmental conditions tested.

Potential Applications

G2379 or its equivalogs could be used to alter a plant's response towater deficit conditions and, therefore, could be used to engineerplants with enhanced tolerance to drought, salt stress, and freezing.G2379 or its equivalogs may also be useful for altering a plant'sresponse to sugars.

G2430 (SEQ ID NO: 435)

Published Information

G2430 was identified in the sequence of BAC F27J15, GenBank accessionnumber AC016041, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G2430 has similarity within of the conserved GARP and response-regulatordomains to non-Arabidopsis proteins.

Experimental Observations

The complete sequence of G2430 (SEQ ID NO: 435) was determined G2430 isa member of the response regulator class of GARP proteins (ARR genes),although one of the two conserved aspartate residues characteristic ofresponse regulators is not present. The second aspartate, the putativephosphorylated site, is retained so G2430 can have response regulatorfunction. G2430 was specifically expressed in embryo and silique tissue.G2430 can regulate plant growth; in morphological analyses, plantsoverexpressing G2430 showed more rapid growth than control plants atearly stages, and in two of three lines examined produced large, flatleaves. Early flowering was observed for some lines, but this effect wasinconsistent between plantings.

Overexpression of G2430 in Arabidopsis resulted in seedlings that aremore tolerant to heat in a germination assay. Seedlings from G2430overexpressing transgenic plants were greener than the control seedlingsunder high temperature conditions. These observations were repeated insubsequent experiments.

Potential Applications

G2430 or its equivalogs may be used to create crops with bettergermination under hot conditions. The germination of many crops is verysensitive to temperature. A gene that would enhance germination in hotconditions may be useful for crops that are planted late in the seasonor in hot climates.

G2430 or its equivalogs can be used to promote faster development andreproduction in plants.

G2505 (SEQ ID NO: 437)

Published Information

G2505 was identified in the sequence of contig fragment No. 29, GenBankaccession number AL161517, released by the Arabidopsis GenomeInitiative.

Experimental Observations

Analysis of the function of G2505 was attempted through the generationtransgenic plants in which the gene was expressed under the control ofthe 35S promoter. Numerous attempts were required to obtain 35S::G2505transformants; thus, overexpression of this gene likely caused lethalityduring embryo or early seedling development. The transformants that wereobtained exhibited improved drought stress tolerance compared to controlplants.

G2505 was expressed in all tissues except shoots and rosette leavesaccording to RT-PCR. No induction of G2505 expression in leaf tissue wasdetected in response to environmental stress related conditions.

Potential Applications

G2505 or its equivalogs could be used to engineer drought hardiness intoseeds or plants, thus providing for improved survival, vigor,appearance, and/or yield in drought stress conditions.

G2509 (SEQ ID NO: 439)

Published Information

G2509 corresponds to gene T2I1_(—)20 (CAB87920).

Closely Related Genes from Other Species

G865 and other non-Arabidopsis AP2/EREBP proteins were similar withinthe conserved AP2 domain.

Experimental Observations

G2509 (SEQ ID NO: 439) was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G2509 caused multiple alterations in plant growth and development,most notably, altered branching patterns, and a reduction in apicaldominance, giving the plants a shorter, more bushy stature than wildtype. Twenty 35S::G2509 primary transformants were examined; at earlystages of rosette development, these plants displayed a wild-typephenotype. However, at the switch to flowering, almost all T1 linesshowed a marked loss of apical dominance and large numbers of secondaryshoots developed from axils of primary rosette leaves. In the mostextreme cases, the shoots had very short internodes, giving theinflorescence a very bushy appearance. Such shoots were often very thinand flowers were relatively small and poorly fertile. At later stages,many plants appeared very small and had a low seed yield compared towild type. In addition to the effects on branching, a substantial numberof 35S::G2509 primary transformants also flowered early and had budsvisible several days prior to wild type. Similar effects oninflorescence development were noted in each of three T2 populationsexamined. The branching and plant architecture phenotypes observed in35S::G2509 lines resemble phenotypes observed for three other AP2/EREBPgenes: G865, G1411, and G1794, G2509, G865, and G1411 form a small cladewithin the large AP2/EREBP family, and G1794, although not belonging tothe clade, is one of the AP2/EREBP genes closest to it in thephylogenetic tree. It is thus likely that all these genes share arelated function, such as affecting hormone balance.

G2509 overexpressing plants had increased seed protein compared towild-type control plants.

Overexpression of G2509 in Arabidopsis resulted in an increase inalpha-tocopherol in seeds in two T2 lines. G2509 was ubiquitouslyexpressed in Arabidopsis plant tissue. G2509 expression levels werealtered by a variety of environmental or physiological conditions.

Potential Applications

G2509 or its equivalogs can be used to manipulate plant architecture anddevelopment.

G2509 or its equivalogs can be used to alter tocopherol composition.

G2509 or its equivalogs can be useful in altering flowering time.

G2517 (SEQ ID NO: 441)

Published Information

G2517 corresponds to gene T12C14_(—)40 (CAB82948).

Closely Related Genes from Other Species

G2517 shows sequence similarity with known genes from other plantspecies within the conserved WRKY domain.

Experimental Observations

G2517 (SEQ ID NO: 441) was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G2517 caused alterations in plant growth and development: sizevariation was apparent in the 35S::G2517 T1 generation, with at leasthalf the lines being very small. Additionally, four of twelve T1 plantsformed flower buds marginally earlier than wild type. Three T1 lineswere examined in the T2 generation, and all three T2 populations wereslightly smaller than controls. In the physiological analysis of the T2populations, G2517 overexpressing lines were more resistant to theherbicide glyphosate.

Potential Applications

G2517 or its equivalogs can be used for the generation of glyphosateresistant plants, and to increase plant resistance to oxidative stress.

G2520 (SEQ ID NO: 443)

Published Information

The sequence of G2520 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AC009317, based on its sequencesimilarity within the conserved domain to other bHLH related proteins inArabidopsis.

Closely Related Genes from Other Species

G2520 shows sequence similarity with known genes from other plantspecies within the conserved basic HLH domain.

Experimental Observations

G2520 (SEQ ID NO: 443) was analyzed using transgenic plants in whichG2520 was expressed under the control of the 35S promoter. At earlystages, 35S::G2520 transformants displayed abnormal curled cotyledons,long hypocotyls, and rather short roots. During the vegetative phase,these plants formed somewhat small flat leaves. Following the switch toreproductive growth, 35S::G2520 inflorescences were typically veryspindly, slightly pale colored, and stems often split open at latestages. Flowers were frequently small with narrow organs and showed poorpollen production. As a result, the seed yield from 35S::G2520 plantswas low compared to wild-type controls. These effects were observed inthe majority of primary transformants, and to varying extents, in allthree of the T2 populations. Overexpression of G2520 also resulted in anincrease in the leaf glucosinolate M39478 in two lines. In addition,these lines showed an increase in seed delta-tocopherol and a decreasein seed gamma-tocopherol. No altered phenotypes were detected in any ofthe physiological assays. G2520 was expressed throughout the plant andwas induced by ABA, heat, salt, drought and osmotic stress.

Potential Applications

G2520 or its equivalogs may be useful for manipulating plant developmentand altering leaf glucosinolate composition.

G2520 or its equivalogs can also be used to modify seed tocopherolcomposition.

G2555 (SEQ ID NO: 445)

Published Information

The sequence of G2555 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AC023064, based on its sequencesimilarity within the conserved domain to other bHLH related proteins inArabidopsis. G2555 corresponds to gene At1g35460/F12A4_(—)2 (AAG52112).

Experimental Observations

The complete sequence of G2555 was determined. G2555 was expressedthroughout the plant, with the highest levels being detected in shoots,flowers, rosette leaves, siliques, and germinating seed. It was notsignificantly induced or repressed by any condition tested.

The function of this gene was analyzed using transgenic plants in whichG2555 was expressed under the control of the 35S promoter.Overexpression of G2555 in Arabidopsis resulted in a small decrease inthe time to flowering. Under continuous light conditions, 35S::G2555transformants produced flower buds and bolted approximately two to fivedays earlier than wild-type controls. Such effects were readily visiblein seven of twenty primary transformants and all plants from two of thethree T2 populations. The third T2 population had only two of six plantsthat flowered early.

G2555 overexpressing seedlings showed open cotyledons when grown in thedark, indicating that G2555 may affect photomorphogenesis. Thisphenotype could be related to the early flowering noted in morphology,if G2555 is involved in light regulation of development. G2555 plantsalso showed increased sensitivity to infection by the necrotrophicfungal pathogen Botrytis cinerea. In repeat experiments on individuallines, all G2555 overexpressing lines showed similar phenotypes. Noaltered phenotypes were detected in any of the biochemical assays.

Potential Applications

G2555 or its equivalogs may be useful for accelerating flowering time incrop plants.

Since G2555 transgenic plants have an altered response to the fungalpathogen Botrytis cinerea, G2555 or its equivalogs might be used tomanipulate the defense response in order to generate pathogen-resistantplants. G2555 or its equivalogs may also be useful for altering someaspect of light-regulated development.

G2557 (SEQ ID NO: 447)

Published Information

The sequence of G2557 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AP001305, based on its sequencesimilarity within the conserved domain to other bHLH related proteins inArabidopsis.

Closely Related Genes from Other Species

G2557 protein shows extensive sequence similarity in the region of basichelix loop helix with a protein encoded by Glycine max cDNA clone(BE347811).

Experimental Observations

G2557 (SEQ ID NO: 447) is a member of a clade of highly related HLH/MYCproteins that also includes G779, G1063, G1499, and G2143. All of thesegenes caused similar pleiotropic phenotypic effects when overexpressed,the most striking of which was the production of ectopic carpelloidtissue. These genes can be considered key regulators of carpeldevelopment. The flowers of 35S::G2557 primary transformants displayedpatches of stigmatic papillae on the sepals, and often had rather narrowpetals and poorly developed stamens. Additionally, carpels were alsooccasionally held outside of the flower at the end of an elongatedpedicel like structure. As a result of such defects, 35S::G2557 plantsoften showed very poor fertility and formed small wrinkled siliques. Inaddition to such floral abnormalities, the majority of primarytransformants were also small and darker green in coloration than wildtype. Approximately one third of the T1 plants were extremely tiny andcompletely sterile. Three T1 lines that had produced some seeds andshowed a relatively weak phenotype were chosen for further study. Allthree of the T2 populations from these lines contained plants that weredistinctly small, had abnormal flowers, and were poorly fertile comparedto controls. Stigmatic tissue was not noted on the sepals of plants fromthese three T2 lines. Another line that had shown a moderately strongphenotype in the T1 was sown for only morphological analysis in the T2generation. These T2 plants were small, dark green, and producedabnormal flowers with ectopic stigmatic tissue on the sepals, as hadbeen seen in the parental plant. G2557 expression was detected at low tomoderate levels in all tissues tested except shoots. G2557 was inducedby cold, heat, and salt, and repressed by pathogen infection

Potential Applications

G2557 or its equivalogs can be used to manipulate flower form andstructure or plant fertility. One application for manipulation of flowerstructure can be in the production of saffron, which is derived from thestigmas of Crocus sativus.

G2583 (SEQ ID NO: 449)

Published Information

G2583 corresponds to gene F2I11_(—)80 (CAB96654).

Closely Related Genes from Other Species

G2583 showed sequence similarity with known genes from other plantspecies within the conserved AP2/EREBP domain.

Experimental Observations

G2583 (SEQ ID NO: 449) was studied using transgenic plants in which thegene was expressed under the control of the 35S promoter. 35S::G2583plants exhibited extremely glossy leaves. At early stages, 35S::G2583seedlings appeared normal, but by about two weeks after sowing, theplants exhibited very striking shiny leaves, which were apparent untilvery late in development. Many lines displayed a variety of othereffects such as a reduction in overall size, narrow curled leaves, orvarious non-specific floral abnormalities, which reduced fertility.These effects on leaf appearance were observed in eighteen of twentyprimary transformants, and in all the plants from four of six of the T2lines examined. The glossy nature of the leaves from 35S::G2583 plantsmay be a consequence of changes in epicuticular wax content orcomposition. G2583 belongs to a small clade within the large AP2/EREBPArabidopsis family that also contains G975, G1387, and G977.Overexpression of G975 caused a substantial increase in leaf waxcomponents, as well as morphological phenotypes resembling thoseobserved in 35S::G2583 plants. G2583 was ubiquitously expressed, athigher levels in root, flower, embryo, and silique tissues.

Potential Applications

G2583 or its equivalogs can be used to modify plant appearance byproducing shiny leaves. In addition, it or its equivalogs can be used tomanipulate wax composition, amount, or distribution, which in turn canmodify plant tolerance to drought and/or low humidity or resistance toinsects.

G2701 (SEQ ID NO: 451)

Published Information

G2701 was identified in the sequence of BAC F11B9, GenBank accessionnumber AC073395, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G2701 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G2701 is Arabidopsis resulted in plants that were wild-type inmorphology and in the biochemical analyses performed. However,35S::G2701 transgenic plants were more tolerant to osmotic stress in agermination assay, the seedlings were greener with expanded cotyledonsand longer roots than wild-type controls when germinated on platescontaining either high salt or high sucrose. The phenotype was repeatedin all three lines.

G2701 was expressed ubiquitously in Arabidopsis according to RT-PCR, andthe level of G2701 expression in leaf tissue was essentially unchangedin response to environmental stress related conditions.

Potential Applications

G2701 or its equivalogs could be used to alter a plant's response towater deficit conditions and therefore, could be used to engineer plantswith enhanced tolerance to drought, salt stress, and freezing.

G2719 (SEQ ID NO: 453)

Published Information

G2719 is a member of the (R1)R2R3 subfamily of MYB transcriptionfactors. G2719 corresponds to gene At3g55730 (AAF72669), and is alsoreferred to as MYB109.

Experimental Observations

The function of G2719 was analyzed using transgenic plants in which thegene was expressed under the control of the 35S promoter. Overexpressionof G2719 in Arabidopsis resulted in plants with increased seedling vigorin a germination assay on media containing high sucrose. This phenotypecould implicate G2719 in sugar sensing and/or osmotic stress tolerance.These observations were seen in repeat experiments. 35S::G2719transgenic plants were wild-type in morphology and in the biochemicalanalyses performed.

G2719 was expressed ubiquitously in Arabidopsis according to RT-PCR, andthe level of G2719 expression in leaf tissue was essentially unchangedin response to environmental stress related conditions.

Potential Applications

G2719 or its equivalogs could be used to alter a plant's response waterdeficit conditions and therefore, could be used to engineer plants withenhanced tolerance to drought, salt stress, and freezing.

In addition, G2719 or its equivalogs could be involved in sugar sensingpathways.

G2789 (SEQ ID NO: 455)

Published Information

The sequence of G2789 was obtained from Arabidopsis genomic sequencingproject, GenBank accession number AL162295, based on its sequencesimilarity within the conserved domain to other AT-hook related proteinsin Arabidopsis. G2789 corresponds to gene T4C21_(—)280 (CAB82691).

Closely Related Genes from Other Species

G2789 protein shows extensive sequence similarity with Medicagotruncatula cDNA clones (AL366947 and BG647144), an Oryza sativachromosome 6 clone (AP003526) and a tomato crown gall Lycopersiconesculentum cDNA clone (BG134451).

Experimental Observations

The complete sequence of G2789 was determined. G2789 was expressed atmoderate levels in roots, flowers, embryos, siliques, and germinatingseeds. It was not detectable in rosette leaves or shoots. No significantinduction of G2789 was observed in rosette leaves by any conditiontested.

The function of this gene was analyzed using transgenic plants in whichG2789 was expressed under the control of the 35S promoter.Overexpression of G2789 in Arabidopsis resulted in seedlings that areABA insensitive and osmotic stress tolerant. In a germination assay onABA containing media, G2789 transgenic seedlings showed enhancedseedling vigor. In a similar germination assay on media containing highconcentrations of sucrose, the G2789 overexpressors also showed enhancedseedling vigor. In a repeat experiment on individual lines, all threelines showed the phenotype. The combination of ABA insensitivity andbetter germination under osmotic stress was also observed for G1820,G926, and G2140. It is possible that ABA insensitivity at thegermination stage promoted germination despite unfavorable conditions.

Overexpression of G2789 produced alterations in leaf and flowerdevelopment, and caused severe reductions in fertility. 35S::G2789primary transformants displayed a variety of leaf abnormalitiesincluding; leaf curling, serrations, and changes in leaf shape and area.The most severely affected individuals grew slowly and were often verytiny compared with wild type. During the reproductive phase, most of thelines showed non-specific defects in flower formation; organs werefrequently absent or poorly developed. As a result of such deficiencies,most of the T1 plants yielded very few seed. A comparable phenotype tothat seen in the T1 was observed in two of the three T2 lines. Someplants from each of these two populations showed a somewhat attenuatedphenotype, suggesting that the transgene might be becoming silenced.Plants from the third line appeared wild type in both the T1 and T2generations.

Overexpression of G2789 in Arabidopsis did not result in any biochemicalphenotypic alteration.

Potential Applications

G2789 or its equivalogs could be used to alter a plant's response towater deficit conditions and therefore, could be used to engineer plantswith enhanced tolerance to drought, salt stress, and freezing.

G2830 (SEQ ID NO: 457)

Published Information

G2830 was identified in the sequence of P1 clone MF020, GenBankaccession number AB013391, released by the Arabidopsis GenomeInitiative.

Experimental Observations

G2830 was primarily expressed at a low level in embryos and siliques asdetermined by RT-PCR analysis. Expression of G2830 was not detected inother tissues. A line homozygous for a T-DNA insertion in G2830 was usedto determine the function of this gene. The T-DNA insertion of G2830 wasapproximately one quarter into the coding sequence of the gene andtherefore is likely to result in a null mutation.

The G2830 knockouts were found to produce more seed oil than wild-typeplants.

Potential Applications

G2830 or its equivalogs can used to increase seed oil content, whichwould be of value for modifying the nutritional value and caloriccontent of food for human consumption as well as animal feeds, and maybe of value in improving seed storage characteristics.

Because expression of G2830 is embryo and silique specific, its promotercould be useful for targeted gene expression in these tissues.

Example IX Identification of Homologous Sequences

This example describes identification of genes that are orthologous toArabidopsis thaliana transcription factors from a computer homologysearch.

Homologous sequences, including those of paralogs and orthologs fromArabidopsis and other plant species, were identified using databasesequence search tools, such as the Basic Local Alignment Search Tool(BLAST) (Altschul et al. (1990) J. Mol. Biol. 215: 403-410; and Altschulet al. (1997) Nucleic Acid Res. 25: 3389-3402). The tblastx sequenceanalysis programs were employed using the BLOSUM-62 scoring matrix(Henikoff and Henikoff (1992) Proc. Natl. Acad. Sci. 89: 10915-10919).The entire NCBI GenBank database was filtered for sequences from allplants except Arabidopsis thaliana by selecting all entries in the NCBIGenBank database associated with NCBI taxonomic ID 33090 (Viridiplantae;all plants) and excluding entries associated with taxonomic ID 3701(Arabidopsis thaliana).

These sequences are compared to sequences representing genes of SEQ IDNO: 2N−1, wherein N=1-229, using the Washington University TBLASTXalgorithm (version 2.0a19MP) at the default settings using gappedalignments with the filter “off”. For each gene of SEQ ID NO: 2N−1,wherein N=1-229, individual comparisons were ordered by probabilityscore (P-value), where the score reflects the probability that aparticular alignment occurred by chance. For example, a score of 3.6e-40is 3.6×10-40. In addition to P-values, comparisons were also scored bypercentage identity. Percentage identity reflects the degree to whichtwo segments of DNA or protein are identical over a particular length.Examples of sequences so identified are presented in Table 7 and Table9. Paralogous or orthologous sequences were readily identified andavailable in GenBank by Accession number (Table 7; Test sequence ID).The percent sequence identity among these sequences can be as low as47%, or even lower sequence identity.

Candidate paralogous sequences were identified among Arabidopsistranscription factors through alignment, identity, and phylogenicrelationships. A list of paralogs is shown in Table 9. Candidateorthologous sequences were identified from proprietary unigene sets ofplant gene sequences in Zea mays, Glycine max and Oryza sativa based onsignificant homology to Arabidopsis transcription factors. Thesecandidates were reciprocally compared to the set of Arabidopsistranscription factors. If the candidate showed maximal similarity in theprotein domain to the eliciting transcription factor or to a paralog ofthe eliciting transcription factor, then it was considered to be anortholog. Identified non-Arabidopsis sequences that were shown in thismanner to be orthologous to the Arabidopsis sequences are provided inTable 7.

Example X Screen of Plant cDNA Library for Sequence Encoding aTranscription Factor DNA Binding Domain that Binds to a TranscriptionFactor Binding Promoter Element and Demonstration of ProteinTranscription Regulation Activity

The “one-hybrid” strategy (Li and Herskowitz (1993) Science 262:1870-1874) is used to screen for plant cDNA clones encoding apolypeptide comprising a transcription factor DNA binding domain, aconserved domain. In brief, yeast strains are constructed that contain alacZ reporter gene with either wild-type or mutant transcription factorbinding promoter element sequences in place of the normal UAS (upstreamactivator sequence) of the GALL promoter. Yeast reporter strains areconstructed that carry transcription factor binding promoter elementsequences as UAS elements are operably linked upstream (5′) of a lacZreporter gene with a minimal GAL1 promoter. The strains are transformedwith a plant expression library that contains random cDNA inserts fusedto the GAL4 activation domain (GAL4-ACT) and screened for blue colonyformation on X-gal-treated filters (X-gal:5-bromo-4-chloro-3-indolyl-β-D-galactoside; Invitrogen Corporation,Carlsbad Calif.). Alternatively, the strains are transformed with a cDNApolynucleotide encoding a known transcription factor DNA binding domainpolypeptide sequence.

Yeast strains carrying these reporter constructs produce low levels ofbeta-galactosidase and form white colonies on filters containing X-gal.The reporter strains carrying wild-type transcription factor bindingpromoter element sequences are transformed with a polynucleotide thatencodes a polypeptide comprising a plant transcription factor DNAbinding domain operably linked to the acidic activator domain of theyeast GAL4 transcription factor, “GAL4-ACT”. The clones that contain apolynucleotide encoding a transcription factor DNA binding domainoperably linked to GLA4-ACT can bind upstream of the lacZ reporter genescarrying the wild-type transcription factor binding promoter elementsequence, activate transcription of the lacZ gene and result in yeastforming blue colonies on X-gal-treated filters.

Upon screening about 2×10⁶ yeast transformants, positive cDNA clones areisolated; i.e., clones that cause yeast strains carrying lacZ reportersoperably linked to wild-type transcription factor binding promoterelements to form blue colonies on X-gal-treated filters. The cDNA clonesdo not cause a yeast strain carrying a mutant type transcription factorbinding promoter elements fused to LacZ to turn blue. Thus, apolynucleotide encoding transcription factor DNA binding domain, aconserved domain, is shown to activate transcription of a gene.

Example XI Gel Shift Assays

The presence of a transcription factor comprising a DNA binding domainwhich binds to a DNA transcription factor binding element is evaluatedusing the following gel shift assay. The transcription factor isrecombinantly expressed and isolated from E. coli or isolated from plantmaterial. Total soluble protein, including transcription factor, (40 ng)is incubated at room temperature in 10 μl of 1× binding buffer (15 mMHEPES (pH 7.9), 1 mM EDTA, 30 mM KCl, 5% glycerol, 5% bovine serumalbumin, 1 mM DTT) plus 50 ng poly(dI-dC):poly(dI-dC) (Pharmacia,Piscataway N.J.) with or without 100 ng competitor DNA. After 10 minutesincubation, probe DNA comprising a DNA transcription factor bindingelement (1 ng) that has been ³²P-labeled by end-filling (Sambrook et al.(1989) supra) is added and the mixture incubated for an additional 10minutes. Samples are loaded onto polyacrylamide gels (4% w/v) andfractionated by electrophoresis at 150V for 2 h (Sambrook et al. supra).The degree of transcription factor-probe DNA binding is visualized usingautoradiography. Probes and competitor DNAs are prepared fromoligonucleotide inserts ligated into the BamHI site of pUC118 (Vieira etal. (1987) Methods Enzymol. 153: 3-11). Orientation and concatenationnumber of the inserts are determined by dideoxy DNA sequence analysis(Sambrook et al. supra). Inserts are recovered after restrictiondigestion with EcoRI and HindIII and fractionation on polyacrylamidegels (12% w/v) (Sambrook et al. supra).

Example XII Introduction of Polynucleotides into Dicotyledonous Plants

Transcription factor sequences listed in the Sequence Listing recombinedinto pMEN20 or pMEN65 expression vectors are transformed into a plantfor the purpose of modifying plant traits. The cloning vector may beintroduced into a variety of cereal plants by means well known in theart such as, for example, direct DNA transfer or Agrobacteriumtumefaciens-mediated transformation. It is now routine to producetransgenic plants using most dicot plants (see Weissbach and Weissbach,(1989) supra; Gelvin et al. (1990) supra; Herrera-Estrella et al. (1983)supra; Bevan (1984) supra; and Klee (1985) supra). Methods for analysisof traits are routine in the art and examples are disclosed above.

Example XIII Transformation of Cereal Plants with an Expression Vector

Cereal plants such as, but not limited to, corn, wheat, rice, sorghum,or barley, may also be transformed with the present polynucleotidesequences in pMEN20 or pMEN65 expression vectors for the purpose ofmodifying plant traits. For example, pMEN020 may be modified to replacethe NptII coding region with the BAR gene of Streptomyces hygroscopicusthat confers resistance to phosphinothricin. The KpnI and BglII sites ofthe Bar gene are removed by site-directed mutagenesis with silent codonchanges.

The cloning vector may be introduced into a variety of cereal plants bymeans well known in the art such as, for example, direct DNA transfer orAgrobacterium tumefaciens-mediated transformation. It is now routine toproduce transgenic plants of most cereal crops (Vasil (1994) Plant Mol.Biol. 25: 925-937) such as corn, wheat, rice, sorghum (Cassas et al.(1993) Proc. Natl. Acad. Sci. 90: 11212-11216, and barley (Wan andLemeaux (1994) Plant Physiol. 104:37-48. DNA transfer methods such asthe microprojectile can be used for corn (Fromm et al. (1990)Bio/Technol. 8: 833-839); Gordon-Kamm et al. (1990) Plant Cell 2:603-618; Ishida (1990) Nature Biotechnol. 14:745-750), wheat (Vasil etal. (1992) Bio/Technol. 10:667-674; Vasil et al. (1993) Bio/Technol.11:1553-1558; Weeks et al. (1993) Plant Physiol. 102:1077-1084), rice(Christou (1991) Bio/Technol. 9:957-962; Hiei et al. (1994) Plant J.6:271-282; Aldemita and Hodges (1996) Planta 199:612-617; and Hiei etal. (1997) Plant Mol. Biol. 35:205-218). For most cereal plants,embryogenic cells derived from immature scutellum tissues are thepreferred cellular targets for transformation (Hiei et al. (1997) PlantMol. Biol. 35:205-218; Vasil (1994) Plant Mol. Biol. 25: 925-937).

Vectors according to the present invention may be transformed into cornembryogenic cells derived from immature scutellar tissue by usingmicroprojectile bombardment, with the A188XB73 genotype as the preferredgenotype (Fromm et al. (1990) Bio/Technol. 8: 833-839; Gordon-Kamm etal. (1990) Plant Cell 2: 603-618). After microprojectile bombardment thetissues are selected on phosphinothricin to identify the transgenicembryogenic cells (Gordon-Kamm et al. (1990) Plant Cell 2: 603-618).Transgenic plants are regenerated by standard corn regenerationtechniques (Fromm et al. (1990) Bio/Technol. 8: 833-839; Gordon-Kamm etal. (1990) Plant Cell 2: 603-618).

The plasmids prepared as described above can also be used to producetransgenic wheat and rice plants (Christou (1991) Bio/Technol.9:957-962; Hiei et al. (1994) Plant J. 6:271-282; Aldemita and Hodges(1996) Planta 199:612-617; and Hiei et al. (1997) Plant Mol. Biol.35:205-218) that coordinately express genes of interest by followingstandard transformation protocols known to those skilled in the art forrice and wheat (Vasil et al. (1992) Bio/Technol. 10:667-674; Vasil etal. (1993) Bio/Technol. 11:1553-1558; and Weeks et al. (1993) PlantPhysiol. 102:1077-1084), where the bar gene is used as the selectablemarker.

Example XIV Identification of Orthologous and Paralogous Sequences

Orthologs to Arabidopsis genes may identified by several methods,including hybridization, amplification, or bioinformatically. Thisexample describes how one may identify equivalogs to the Arabidopsis AP2family transcription factor CBF1 (polynucleotide SEQ ID NO: 1955,encoded polypeptide SEQ ID NO: 1956), which confers tolerance to abioticstresses (Thomashow et al. (2002) U.S. Pat. No. 6,417,428), and anexample to confirm the function of homologous sequences. In thisexample, orthologs to CBF1 were found in canola (Brassica napus) usingpolymerase chain reaction (PCR).

Degenerate primers were designed for regions of AP2 binding domain andoutside of the AP2 (carboxyl terminal domain):

Mol 368 (reverse) (SEQ ID NO: 2205) 5′- CAY CCN ATH TAY MGN GGN GT -3′Mol 378 (forward) (SEQ ID NO: 2206) 5′- GGN ARN ARC ATN CCY TCN GCC -3′(Y: C/T, N: A/C/G/T, H: A/C/T, M: A/C, R: A/G )

Primer Mol 368 is in the AP2 binding domain of CBF1 (amino acidsequence: His-Pro-Ile-Tyr-Arg-Gly-Val, SEQ ID NO: 2909) while primer Mol378 is outside the AP2 domain (carboxyl terminal domain) (amino acidsequence: Met-Ala-Glu-Gly-Met-Leu-Leu-Pro, SEQ ID NO: 2910).

The genomic DNA isolated from B. napus was PCR-amplified by using theseprimers following these conditions: an initial denaturation step of 2min at 93° C.; 35 cycles of 93° C. for 1 min, 55° C. for 1 min, and 72°C. for 1 min; and a final incubation of 7 min at 72° C. at the end ofcycling.

The PCR products were separated by electrophoresis on a 1.2% agarose geland transferred to nylon membrane and hybridized with the AT CBF1 probeprepared from Arabidopsis genomic DNA by PCR amplification. Thehybridized products were visualized by colorimetric detection system(Boehringer Mannheim) and the corresponding bands from a similar agarosegel were isolated using the Qiagen Extraction Kit (Qiagen). The DNAfragments were ligated into the TA clone vector from TOPO TA Cloning Kit(Invitrogen) and transformed into E. coli strain TOP10 (Invitrogen).

Seven colonies were picked and the inserts were sequenced on an ABI 377machine from both strands of sense and antisense after plasmid DNAisolation. The DNA sequence was edited by sequencer and aligned with theAtCBF1 by GCG software and NCBI blast searching.

The nucleic acid sequence and amino acid sequence of one canola orthologfound in this manner (bnCBF1; polynucleotide SEQ ID NO: 2203 andpolypeptide SEQ ID NO: 2204) identified by this process is shown in theSequence Listing.

The aligned amino acid sequences show that the bnCBF1 gene has 88%identity with the Arabidopsis sequence in the AP2 domain region and 85%identity with the Arabidopsis sequence outside the AP2 domain whenaligned for two insertion sequences that are outside the AP2 domain.

Similarly, paralogous sequences to Arabidopsis genes, such as CBF1, mayalso be identified.

Two paralogs of CBF1 from Arabidopsis thaliana: CBF2 and CBF3. CBF2 andCBF3 have been cloned and sequenced as described below. The sequences ofthe DNA SEQ ID NO: 1957 and 1959 and encoded proteins SEQ ID NO: 1958and 1960 are set forth in the Sequence Listing.

A lambda cDNA library prepared from RNA isolated from Arabidopsisthaliana ecotype Columbia (Lin and Thomashow (1992) Plant Physiol. 99:519-525) was screened for recombinant clones that carried insertsrelated to the CBF1 gene (Stockinger et al. (1997) Proc. Natl. Acad.Sci. 94:1035-1040). CBF1 was ³²P-radiolabeled by random priming(Sambrook et al. supra) and used to screen the library by theplaque-lift technique using standard stringent hybridization and washconditions (Hajela et al. (1990) Plant Physiol. 93:1246-1252; Sambrooket al. supra) 6×SSPE buffer, 60° C. for hybridization and 0.1×SSPEbuffer and 60° C. for washes). Twelve positively hybridizing clones wereobtained and the DNA sequences of the cDNA inserts were determined. Theresults indicated that the clones fell into three classes. One classcarried inserts corresponding to CBF1. The two other classes carriedsequences corresponding to two different homologs of CBF1, designatedCBF2 and CBF3. The nucleic acid sequences and predicted protein codingsequences for Arabidopsis CBF1, CBF2 and CBF3 are listed in the SequenceListing (SEQ ID NOs: 1955, 1957, 1959 and SEQ ID NOs: 1956, 1958, 1960,respectively). The nucleic acid sequences and predicted protein codingsequence for Brassica napus CBF ortholog is listed in the SequenceListing (SEQ ID NOs: 2203 and 2204, respectively).

A comparison of the nucleic acid sequences of Arabidopsis CBF1, CBF2 andCBF3 indicate that they are 83 to 85% identical as shown in Table 11.

TABLE 11 Percent identity^(a) DNA^(b) Polypeptide cbf1/cbf2 85 86cbf1/cbf3 83 84 cbf2/cbf3 84 85 ^(a)Percent identity was determinedusing the Clustal algorithm from the Megalign program (DNASTAR, Inc.).^(b)Comparisons of the nucleic acid sequences of the open reading framesare shown.

Similarly, the amino acid sequences of the three CBF polypeptides rangefrom 84 to 86% identity. An alignment of the three amino acidicsequences reveals that most of the differences in amino acid sequenceoccur in the acidic C-terminal half of the polypeptide. This region ofCBF1 serves as an activation domain in both yeast and Arabidopsis (notshown).

Residues 47 to 106 of CBF1 correspond to the AP2 domain of the protein,a DNA binding motif that to date, has only been found in plant proteins.A comparison of the AP2 domains of CBF1, CBF2 and CBF3 indicates thatthere are a few differences in amino acid sequence. These differences inamino acid sequence might have an effect on DNA binding specificity.

Example XV Transformation of Canola with a Plasmid Containing CBF1,CBF2, or CBF3

After identifying homologous genes to CBF1, canola was transformed witha plasmid containing the Arabidopsis CBF1, CBF2, or CBF3 genes clonedinto the vector pGA643 (An (1987) Methods Enzymol. 253: 292). In theseconstructs the CBF genes were expressed constitutively under the CaMV35S promoter. In addition, the CBF1 gene was cloned under the control ofthe Arabidopsis COR15 promoter in the same vector pGA643. Each constructwas transformed into Agrobacterium strain GV3101. TransformedAgrobacteria were grown for 2 days in minimal AB medium containingappropriate antibiotics.

Spring canola (B. napus cv. Westar) was transformed using the protocolof Moloney et al. (1989) Plant Cell Reports 8: 238, with somemodifications as described. Briefly, seeds were sterilized and plated onhalf strength MS medium, containing 1% sucrose. Plates were incubated at24° C. under 60-80 μE/m²s light using a 16 hour light/8 hour darkphotoperiod. Cotyledons from 4-5 day old seedlings were collected, thepetioles cut and dipped into the Agrobacterium solution. The dippedcotyledons were placed on co-cultivation medium at a density of 20cotyledons/plate and incubated as described above for 3 days. Explantswere transferred to the same media, but containing 300 mg/l timentin(SmithKline Beecham, Pa.) and thinned to 10 cotyledons/plate. After 7days explants were transferred to Selection/Regeneration medium.Transfers were continued every 2-3 weeks (2 or 3 times) until shoots haddeveloped. Shoots were transferred to Shoot-Elongation medium every 2-3weeks. Healthy looking shoots were transferred to rooting medium. Oncegood roots had developed, the plants were placed into moist pottingsoil.

The transformed plants were then analyzed for the presence of the NPTIIgene/kanamycin resistance by ELISA, using the ELISA NPTII kit from5Prime-3Prime Inc. (Boulder, Colo.). Approximately 70% of the screenedplants were NPTII positive. Only those plants were further analyzed.

From Northern blot analysis of the plants that were transformed with theconstitutively expressing constructs, showed expression of the CBF genesand all CBF genes were capable of inducing the Brassica napuscold-regulated gene BN115 (homolog of the Arabidopsis COR15 gene). Mostof the transgenic plants appear to exhibit a normal growth phenotype. Asexpected, the transgenic plants are more freezing tolerant than thewild-type plants. Using the electrolyte leakage of leaves test, thecontrol showed a 50% leakage at −2 to −3° C. Spring canola transformedwith either CBF1 or CBF2 showed a 50% leakage at −6 to −7° C. Springcanola transformed with CBF3 shows a 50% leakage at about −10 to −15° C.Winter canola transformed with CBF3 may show a 50% leakage at about −16to −20° C. Furthermore, if the spring or winter canola are coldacclimated the transformed plants may exhibit a further increase infreezing tolerance of at least −2° C.

To test salinity tolerance of the transformed plants, plants werewatered with 150 mM NaCl. Plants overexpressing CBF1, CBF2 or CBF3 grewbetter compared with plants that had not been transformed with CBF1,CBF2 or CBF3.

These results demonstrate that equivalogs of Arabidopsis transcriptionfactors can be identified and shown to confer similar functions innon-Arabidopsis plant species.

Example XVI Cloning of Transcription Factor Promoters

Promoters are isolated from transcription factor genes that have geneexpression patterns useful for a range of applications, as determined bymethods well known in the art (including transcript profile analysiswith cDNA or oligonucleotide microarrays, Northern blot analysis,semi-quantitative or quantitative RT-PCR). Interesting gene expressionprofiles are revealed by determining transcript abundance for a selectedtranscription factor gene after exposure of plants to a range ofdifferent experimental conditions, and in a range of different tissue ororgan types, or developmental stages. Experimental conditions to whichplants are exposed for this purpose includes cold, heat, drought,osmotic challenge, varied hormone concentrations (ABA, GA, auxin,cytokinin, salicylic acid, brassinosteroid), pathogen and pestchallenge. The tissue types and developmental stages include stem, root,flower, rosette leaves, cauline leaves, siliques, germinating seed, andmeristematic tissue. The set of expression levels provides a patternthat is determined by the regulatory elements of the gene promoter.

Transcription factor promoters for the genes disclosed herein areobtained by cloning 1.5 kb to 2.0 kb of genomic sequence immediatelyupstream of the translation start codon for the coding sequence of theencoded transcription factor protein. This region includes the 5′-UTR ofthe transcription factor gene, which can comprise regulatory elements.The 1.5 kb to 2.0 kb region is cloned through PCR methods, using primersthat include one in the 3′ direction located at the translation startcodon (including appropriate adaptor sequence), and one in the 5′direction located from 1.5 kb to 2.0 kb upstream of the translationstart codon (including appropriate adaptor sequence). The desiredfragments are PCR-amplified from Arabidopsis Col-0 genomic DNA usinghigh-fidelity Taq DNA polymerase to minimize the incorporation of pointmutation(s). The cloning primers incorporate two rare restriction sites,such as Not1 and Sfi1, found at low frequency throughout the Arabidopsisgenome. Additional restriction sites are used in the instances where aNot1 or Sfi1 restriction site is present within the promoter.

The 1.5-2.0 kb fragment upstream from the translation start codon,including the 5′-untranslated region of the transcription factor, iscloned in a binary transformation vector immediately upstream of asuitable reporter gene, or a transactivator gene that is capable ofprogramming expression of a reporter gene in a second gene construct.Reporter genes used include green fluorescent protein (and relatedfluorescent protein color variants), beta-glucuronidase, and luciferase.Suitable transactivator genes include LexA-GAL4, along with atransactivatable reporter in a second binary plasmid (as disclosed inU.S. patent application Ser. No. 09/958,131, incorporated herein byreference). The binary plasmid(s) is transferred into Agrobacterium andthe structure of the plasmid confirmed by PCR. These strains areintroduced into Arabidopsis plants as described in other examples, andgene expression patterns determined according to standard methods knowto one skilled in the art for monitoring GFP fluorescence,beta-glucuronidase activity, or luminescence.

All references, publications, patent documents, web pages, and otherdocuments cited or mentioned herein are hereby incorporated by referencein their entirety for all purposes. Although the invention has beendescribed with reference to specific embodiments and examples, it shouldbe understood that one of ordinary skill can make various modificationswithout departing from the spirit of the invention. The scope of theinvention is not limited to the specific embodiments and examplesprovided.

What is claimed is:
 1. A method for producing a plant having an alteredtrait with respect to a control plant, the method comprising introducinginto a plant a recombinant polynucleotide encoding a polypeptide; (a)wherein the polypeptide is at least 70% identical to SEQ ID NO: 214; andcomprises a conserved domain sharing at least 98% amino acid identity toamino acid residues 201-261 of SEQ ID NO: 214, wherein when saidpolypeptide is overexpressed in a plant, said overexpression of thepolypeptide confers increased yield, later flowering, increased biomass,larger leaves, or altered leaf prenyl lipid content to the transgenicplant as compared to the control plant.
 2. The method of claim 1,wherein expression of the polypeptide is regulated by a tissue-specific,inducible, or constitutive promoter.
 3. The method of claim 1, whereinthe method optionally includes the steps of: (a) crossing the plant withitself or another plant; (b) selecting seed that develops as a result ofsaid crossing; and (c) growing a progeny plant from the seed, whereinthe seed comprises the DNA construct.
 4. A transgenic plant having analtered trait with respect to a control plant, wherein the transgenicplant comprises a recombinant polynucleotide encoding a polypeptide;wherein the polypeptide comprises a conserved domain that shares atleast 98% amino acid identity to amino acid residues 201-261 of SEQ IDNO:
 214. 5. The transgenic plant of claim 4, wherein the polypeptide isat least 70% identical to the full length sequence of SEQ ID NO:
 214. 6.The transgenic plant of claim 4, wherein the polypeptide is at least 72%identical to the full length sequence of SEQ ID NO:
 214. 7. Thetransgenic plant of claim 6, wherein the polypeptide is SEQ ID NO: 214.8. The transgenic plant of claim 4, wherein when said polypeptide isoverexpressed in a plant, said overexpression of the polypeptide confersincreased yield, later flowering, increased biomass, larger leaves, oraltered leaf prenyl lipid content to the transgenic plant as compared toa control plant.
 9. The transgenic plant of claim 4, wherein recombinantpolynucleotide is operably-linked to a tissue-specific, inducible, orconstitutive promoter.
 10. A transgenic seed produced from the plant ofclaim 4, wherein the seed comprises the recombinant polynucleotide.